CN101320015A - Method for fast detecting vitamin E content - Google Patents
Method for fast detecting vitamin E content Download PDFInfo
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- CN101320015A CN101320015A CNA2008101169416A CN200810116941A CN101320015A CN 101320015 A CN101320015 A CN 101320015A CN A2008101169416 A CNA2008101169416 A CN A2008101169416A CN 200810116941 A CN200810116941 A CN 200810116941A CN 101320015 A CN101320015 A CN 101320015A
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Abstract
The invention relates to a poly-pyrrole modified electrode doped with p-toluene sulphonic acid and provides a method using the modified electrode for rapidly detecting the content of vitamin E. the method comprises: 1) using experimental conditions which are the same with the detecting conditions of a sampler for drawing a standard curve of the content of vitamin E and oxidation peak current; 2) dissolving the sample in the mixed solvent of ethanol-1 and 2 dichloroethane and regulating the pH value to be 2-5 to obtain the sample solution; 3) arranging the modified electrode in the sample solution, detecting the oxidation peak current through a differential pulse voltammetry and calculating the concentration of vitamin E in the sample. The method can be used for rapidly detecting the content of vitamin E in vegetable oil and has the advantages of simple sample preparation, convenience, fast speed, strong anti-interference ability and accurate detecting result which is identical with the detecting result of a liquid chromatography.
Description
Technical field
The present invention relates to the Food Science detection range.The method that particularly relates to a kind of fast detecting content of vitamin E.
Background technology
Vitamin E extensively is present in animals and plants circle, particularly crop seeds, the vegetable oil as a kind of natural, nontoxic, strong effectively phenol antioxidant, and the absorption of vegetable oil is the important source of needed by human body vitamin E in the daily meals.Scientific experiment shows that vitamin E plays an important role, and especially fertility is had significant effects in the body vital movement, therefore be referred to as tocopherol again, can be divided into α, beta, gamma, δ, ε, ζ, η, eight kinds of hypotypes of θ are wherein the strongest with the effect of alpha-tocopherol; As the fat-soluble antioxidant that is positioned film; it can effectively suppress the generation of free radical; the damage that stops the radical pair body to cause, tissues such as protection cell membrane, skin, blood vessel, heart, eyes, liver and breast reduce the probability of suffering from diseases such as artery sclerosis.The content of vitamin E not only embodies its nutritive value in edible oil and the oil-containing food, and its oxidation stability and shelf life are had significant effects.When the content of vitamin E reaches 30mg/100g in the edible oil, just can effectively delay lipid oxidation, prolong the shelf life of edible oil.Therefore fast, accurately analysis determines that the content of vitamin E in edible oil and the oil-containing food is estimated its nutritional quality and stability assessment is quite important.
The vitamin E complex structure, the isomeride kind is many, accurately assay determination is relatively more difficult, domestic and international in recent years many laboratories and researcher are devoted to the research of its assay determination, obtained impressive progress, developed multiple useful analytical approach, mainly contain: cerimetry, spectrophotometric method, fluorescence method, near infrared spectroscopy, vapor-phase chromatography, high performance liquid chromatography and electrochemical methods etc., these methods respectively have relative merits, and its sensitivity, accuracy, detection required time and applicable situation scope have nothing in common with each other.Traditional chemical method detection sensitivity is low, and needed chemical reagent is many; Disturb advantages such as little, highly sensitive, quick though chromatography is compared with other method to have, pre-treatment requires high, and processing procedure is comparatively complicated, consuming time longer, detects the cost height, is confined to the laboratory more and detects.In view of above reason, domestic and international many laboratories seeking always vitamin E more easy, fast, detection method cheaply, mainly concentrate on the application of electrochemical analysis method in vitamin E detects.Liu Mengs etc. utilize single-sweep oscillographic polarography to measure vitamin content, with Ce (SO
4)
2Be oxygenant, earlier vitamin be oxidized to its quinoid compound, then at NH
3H
2Carry out cathodic reduction on the electrode at the bottom of the O ethanol in the liquid, this quinoid compound has a sharp reduction peak in-0.29V place, and peak current size and vitamine concentration are 1.2 * 10
-5～4.5 * 10
-1Present the good linear relation in the mol/L scope, and under selected condition, can not disturb vitamin to measure when 4.5 times of phenol, 3 times of coexistences such as p-dihydroxy-benzene.McBride and Evans etc. are end liquid with ethanol and benzene, and vegetable oil directly is dissolved in end liquid, utilize linear scan technique to analyze the content of various tocopherols (vitamin E) in the vegetable oil.
At present, domestic and international research is mainly used bare electrode and is detected vitamin E in the vegetable oil, modified electrode is not applied to the report of the detection of vegetable oil vitamin E.The inventor is through research, prepare a kind of polypyrrole modifying platinum electrode with good stability, and propose based on the method for this electrode with the content of vitamin E in the voltammetry detection vegetable oil, the method specimen preparation is simple, fast convenient, anti-interference strong, testing result is accurate, and is consistent with the testing result of liquid phase chromatography.
Summary of the invention
The method for quick that the purpose of this invention is to provide a kind of detection of the vitamin E that modified electrode is used for vegetable oil.
Another object of the present invention provides a kind of modified electrode, and described modified electrode is a kind of good stability, and is anti-interference strong, but the polypyrrole modifying platinum electrode of the content of vitamin E in the fast detecting vegetable oil provides the method for making of this electrode simultaneously.
The polypyrrole modifying electrode can with common electrochemical method with pyrroles's polymer deposits on base electrode, preferred manufacturing procedure is as follows:
1) with platinum electrode as base electrode, through cleaning surfaces, dry back is stand-by;
2) acetonitrile solution that platinum electrode is placed pyrrole monomer and p-toluenesulfonic acid form, between the current potential of 0.8～1.0V, utilize electrochemical workstation to adopt constant potential method polymerization 80～120s, make platinum electrode surface form pyrroles's condensate of doping p-toluenesulfonic acid.Wherein, pyrroles's concentration is 0.02mol/L in the acetonitrile solution, and the concentration of p-toluenesulfonic acid is 0.02mol/L; The operating characteristic of electrochemical workstation is: three electrode work systems, contrast electrode are Ag/AgCl, and auxiliary electrode is a platinum electrode, and working electrode is a platinum electrode.
This electrode is used for the detection of edible vegetable oil content of vitamin E:
1) utilize calibration curve method to obtain the typical curve (concrete experiment condition is identical with the sample detection condition) of vitamin E detection;
2) take by weighing 0.5-2g edible vegetable oil and be dissolved in the mixed solvent of ethanol-1,2 ethylene dichloride, regulating the pH value is 2-5, makes sample liquid;
3) above-mentioned modified electrode is placed sample liquid, detect oxidation peak current (Ip), calculate vitamin E concentration in the sample according to typical curve with the differentiated pulse voltammetry.
By experiment, the preferred detection condition is:
1) utilize calibration curve method to obtain the typical curve (concrete experiment condition is identical with the sample detection condition) of vitamin E detection, the purity of vitamin E standard items is 95%;
2) take by weighing the 0.5-2g vegetable oil be dissolved in ethanol-1,2 ethylene dichloride mixed solvent (3: 1, V/V) in, wherein add supporting electrolyte: LiClO
4, its concentration is 0.02～0.1mol/L, uses H
2SO
4It is 2-5 that ethanolic solution is regulated the pH value, makes sample liquid;
3) above-mentioned electrode is placed sample liquid, sample liquid N
2Purifying 5 minutes is at N
2Atmosphere detects oxidation peak current (Ip) with the differentiated pulse voltammetry, and initial potential is 0.2V, and whole current potential is 0.8V, and sweep velocity is 10～50mV/s, and pulse height is 50mV, calculates vitamin E concentration (C in the sample according to typical curve
α-T).
Under same experiment condition, adopting this modified electrode and unmodified platinum electrode respectively is working electrode, in electrochemical cell, carry out the electrochemical reaction of vitamin E, relatively find: this modified electrode responds the response that is far longer than the unmodified platinum electrode to the oxidation current of vitamin E, and experimental results show that the doped polypyrrole P-TOLUENE SULFO ACID 99 who prepares through potentiostatic method modifies platinum electrode and has good stable and repeatability, its result shows as: use the constant potential polymerization and make 11 modified electrodes (with platinum electrode as base electrode, place the acetonitrile solution of pyrrole monomer and p-toluenesulfonic acid, under the constant potential of 0.9V, utilize electrochemical workstation to adopt constant potential method polymerization 80s), detectable concentration is 2.3 * 10
-4The vitamin E of mol/L, the mean value of the oxidation peak current that obtains be 12.31 ± 0.35 μ A (n=11, RSD=2.8%); The electrode newly modified and the same modified electrode deposited one month under dry, lucifuge condition are detected the vitamin E of same concentration, the oxidation peak current that relatively obtains, the latter's oxidation peak current only descends 3.8%.
Owing to also have other polyphenoils usually in the vegetable oil; as butylhydroxy anisole (BHA), 2; 6-BHT (BHT) and ditert-butylhydro quinone (THBQ); when using electrochemical process to measure content of vitamin E in the past; owing to the also oxidized generation oxidation peak of these antioxidants, often disturb the testing result of content of vitamin E.Doped polypyrrole P-TOLUENE SULFO ACID 99's modified electrode that the present invention adopts has good anti-interference, its electrochemical reaction responds, experimental results show that: to vitamin E, butylhydroxy anisole (BHA), 2, the response of 6-BHT (BHT) and ditert-butylhydro quinone (THBQ) oxidation is obvious, disturbs little.
Use the content of vitamin E in above-mentioned detection method and the electrode detection vegetable oil, its result is consistent with the testing result of liquid phase chromatography, illustrates that detection method result of the present invention is accurate, is applicable to the detection of the content of vitamin E in the vegetable oil.
The doped polypyrrole P-TOLUENE SULFO ACID 99's modified electrode good stability that the present invention relates to, anti-interference strong, method for making is simple, and the electrochemical oxidation of vitamin E is had significant catalytic action, and the oxidation response obviously is applicable to the content of vitamin E in the fast detecting vegetable oil.
The application modified electrode that the present invention relates to detects in the method for content of vitamin E in the vegetable oil, and edible oil does not need directly to be dissolved in the simple solvent and can to detect through complicated pre-treatment; Each sample detection only needs 80s, and is rapidly convenient; Testing result is consistent with high performance liquid chromatography, and testing result is accurate, and this method can fully satisfy easy, the requirement fast and accurately that the vegetable oil content of vitamin E detects.
Description of drawings
Fig. 1 represents that modified electrode of the present invention measures the typical curve of oxidation current response of the vitamin E standard solution of variable concentrations.
Fig. 2 represents to detect with modified electrode of the present invention the differentiated pulse voltammogram of rape seed oil content of vitamin E.
The comparative result of the cyclic voltammogram when Fig. 3 represents with modified electrode of the present invention and bare electrode detection same concentrations vitamin E, wherein a is a unmodified platinum electrode measurement result, b is for modifying the platinum electrode measurement result.
Fig. 4 represents the anti-interference effect of modified electrode of the present invention.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.The electrochemistry experiment that detects content of vitamin E removes and specifies, all carries out under 25 ± 0.5 ℃ of following nitrogen atmospheres.
Place acetone to soak 1 hour platinum electrode, ultrasonic cleaning 5 minutes is used deionized water rinsing then, and dry back is stand-by;
Configuration pyrrole monomer concentration is 0.02mol/L, and p-toluenesulfonic acid concentration is the modification solution of the acetonitrile solution of 0.02mol/L as platinum electrode;
Platinum electrode is placed above-mentioned modification solution, set current potential 0.9V, adopt potentiostatic method, utilize the CHI 650C electrochemical workstation polymerization 80s of Shanghai occasion China instrument company, electrochemical workstation adopts three electrode work systems, and contrast electrode is Ag/AgCl (U.S. CHI instrument company), and auxiliary electrode is a platinum electrode, working electrode is a platinum electrode, the polypyrrole modifying platinum electrode of preparation doping p-toluenesulfonic acid.
The content of vitamin E that the polypyrrole modifying platinum electrode of the doping p-toluenesulfonic acid that makes is used for detecting vegetable oil.
1) typical curve of content of vitamin E and oxidation peak current: get vitamin E standard items (purity is 95%, Merck company (Germany)), configuration concentration is respectively 6,20,50,75,125,180,230,275,300 μ mol/L vitamin E standard solution.Wherein ethanol-1,2 ethylene dichloride mixing (3: 1, V/V) as solvent, supporting electrolyte LiClO
4Concentration be 0.05mol/L, with the H of 0.1mol/L
2SO
4Regulating the pH value is 4.0, places electrochemical cell, charges into high purity nitrogen deoxygenation gas; With the polypyrrole modifying platinum electrode of the doping p-toluenesulfonic acid that makes as working electrode, platinum filament is as auxiliary electrode, Ag/AgCl is as contrast electrode, detect oxidation peak current (Ip) with the differentiated pulse voltammetry, initial potential is 0.2V, whole current potential is 0.8V, and sweep velocity is 10mV/s, and pulse height is 50mV; Utilize Microsoft Excel to handle the data that obtain, obtain the typical curve (Ip=0.246+0.0538C of content of vitamin E
α-T) (see figure 1).
2) take by weighing 1,2 ethylene dichloride that the 2g edible rapeseed oil is dissolved in 12.5mL, wherein add supporting electrolyte: LiClO
4Ethanolic solution, make LiClO
4Ultimate density is 0.05mol/L, adds the 0.1mol/L H of 25 μ L
2SO
4Ethanolic solution, making solution final pH value is 4, at last with the ethanol constant volume to 50ml;
3) the polypyrrole modifying platinum electrode with the doping p-toluenesulfonic acid places sample liquid, detect oxidation peak current (Ip) with the differentiated pulse voltammetry, initial potential is 0.2V, whole current potential is 0.8V, sweep velocity is 10mV/s, and pulse height is 50mV, and the differentiated pulse voltammogram that obtains is seen Fig. 2, oxidation peak current is 3.44 μ A, calculates vitamin E concentration (C in the sample according to typical curve
α-T) be 59.43 μ mol/L, content of vitamin E is 64mg/100g in the rapeseed oil.
Method with embodiment 1 is made 11 modified electrodes, detects 2.3 * 10
-4The oxidation peak current value of the vitamin E of mol/L, the mean value that obtains be 12.31 ± 0.35 μ A (n=11, RSD=2.8%).The result is as shown in table 1.
11 modified electrodes that table 1 adopts identical preparation method to obtain detect the vitamin E current value
Measure freshly prepd modified electrode respectively and under dry, lucifuge condition, deposit one month same modified electrode oxidation peak current and contrast, the result shows that the latter's oxidation peak current has descended 3.8%.
The contrast of embodiment 4 modified electrodes and unmodified bare electrode
The vitamin E sample of application standard (purity is 95%, Merck company (Germany)), configuration vitamin E solution is 2.3 * 10 in the vitamin E solution concentration
-4During mol/L, carry out cyclic voltammetry scan with the polypyrrole modifying platinum electrode of doping p-toluenesulfonic acid and the platinum electrode of unmodified respectively, observe their oxidation current response, the results are shown in Figure 3, wherein a is the platinum electrode measurement result of unmodified, and b is for modifying the platinum electrode measurement result.Experiment is found: on modified electrode, the vitamin E oxidation peak current has enlarged markedly about 8～9 times, and the oxidation peak current potential moves about 160mV to the negative potential direction, shows that this modified electrode has significant catalytic action to the electrochemical oxidation of vitamin E.
This embodiment be for illustrate this method in edible vegetable oil vitamin E testing process to being present in the anti-interference effect of other antioxidants in the edible vegetable oil.
Specific implementation method is with common several antioxidants such as butylhydroxy anisole (BHA), 2,6-BHT (BHT) and ditert-butylhydro quinone (THBQ) add in the solution system of vitamin E, for ease of research, adopt vitamin E standard items configuration vitamin E solution, 5.0 * 10
-5Mol/L TBHQ, BHA and 1.5 * 10
-4Survey the recovery of the vitamin E of variable concentrations when mol/L BHT exists simultaneously with the inventive method, the result is as shown in table 2, and the result shows that this method has good anti-interference effect.
Table 2 is according to calibration curve method, the recovery of the measurement result of the vitamin E when TBHQ, BHA, BHT coexistence
Annotate: the concentration of TBHQ is: 5.0 * 10
-5M; The concentration of BHA is: 5.0 * 10
-5M; The concentration of BHT is: 1.5 * 10
-4M
Fig. 4 is 5.0 * 10
-5Mol/L TBHQ, 5.0 * 10
-5Mol/L BHA, 1.5 * 10
-4Mol/L BHT and 6 * 10
-5Mol/L vitamin E (standard items) is when existing jointly, the oxidation peak current of each material that employing differentiated pulse voltammetry method records, wherein used sweep velocity is 10mV/S, pulse height is 50mV, as seen the peak current of gained vitamin E is 3.58 μ A, and the concentration that calculates vitamin E according to typical curve is 6.2 * 10
-5Mol/L, very approaching with the actual concentrations of vitamin E in the sample, show good in anti-interference performance when the present invention detects vitamin E.
1) content of vitamin E in the employing high-efficient liquid phase chromatogram technique measuring edible vegetable oil
1. chromatographic condition: stationary phase: A Nova pak C18 post (4 μ m particles, the Dalian China chromatogram center of 15cm * 4.6mm); Moving phase is methyl alcohol and water (98: 2), and flow velocity is 0.8mL/min, and sample size is: 10 μ L; Fluorescence detector: excitation wavelength 295nm, emission wavelength 325nm; Column temperature: 25 ℃; Chromatogram is by Tianjin, island C-R7A type registering instrument record.
2. sample determination: accurately take by weighing soybean oil, sunflower oil, rape seed oil, corn oil, each 5g of wheat-germ oil and sesame oil, place the separating funnel of 50mL respectively, with extracted by ether three times, each 10mL, merge ether extracted liquid, place evaporate to dryness ether in the water-bath, the residue n-hexane dissolution moves in the volumetric flask of 10mL after filtering with the filter membrane of 0.45 μ m, is diluted to scale with normal hexane again, shake up, draw 10 μ L sample introductions, measure the extraction treating fluid of above oil sample respectively with above-mentioned chromatographic condition, each sample carries out three replicate determinations, calculate the content of vitamin E in each oil sample with the external standard peak area method, testing result sees Table 3.
2) employing this method is measured the content of vitamin E in the edible vegetable oil
The food plant oil samples need not any pre-service.Take by weighing each 1g of soybean oil, sunflower oil, rape seed oil, corn oil, wheat-germ oil and sesame oil, place the volumetric flask of 25mL, with the dissolving of 1,2 ethylene dichloride, adding ethanol sulfuric acid solution adjustment pH value subsequently is 4, supporting electrolyte LiClO
4Concentration is 0.05mol/L, is diluted with ethanol to final scale then, and the volume ratio of last ethanol and 1,2 ethylene dichloride is 3: 1, makes sample solution.Sample solution is joined in the electrochemical cell, stir and use N
2Purifying 5min detects with the differentiated pulse voltammetry then, and wherein sweep velocity is 10mV/S, and pulse height is 50mV, writes down its voltammogram, and each oil sample is done three parallel laboratory tests, and the mean value of getting three experiments is as measurement result.Remove and specify that all tests are all carried out under 25 ± 0.5 ℃, all carry out under nitrogen atmosphere simultaneously, the result is as shown in table 3.
Content of vitamin E and HPLC that table 3 this method is surveyed record content of vitamin E
According to the result of table 3, relatively high performance liquid chromatography and the present invention detect the testing result of content of vitamin E method, and obvious two kinds the resulting result of method is close, difference is less, and this method is in measuring process, and sample does not need through any and processing.Adopt this detection method not only easily and fast, and the result is accurate.
Claims (10)
1, a kind of method of fast detecting content of vitamin E can detect the content of vitamin E in the vegetable oil, comprising:
1) ethanol-1 of the vitamin E standard items of preparation variable concentrations, 2 dichloroethane solutions are regulated its pH value and are 2-5, adopt the polypyrrole modifying electrode of doping p-toluenesulfonic acid, detect with the differentiated pulse voltammetry, obtain the typical curve of vitamin E oxidation peak current and content;
2) sample thief is dissolved in the mixed solvent of ethanol-1,2 ethylene dichloride, and regulating the pH value is 2-5, makes sample liquid;
3) the polypyrrole modifying electrode with the doping p-toluenesulfonic acid places sample liquid, detects oxidation peak current with the differentiated pulse voltammetry, calculates vitamin E concentration in the sample according to typical curve.
2, the method for claim 1 is characterized in that, containing concentration in the mixed solvent of described ethanol-1,2 ethylene dichloride is the supporting electrolyte LiClO of 0.02～0.1mol/L
4
3, the method for claim 1 is characterized in that, the volume ratio of ethanol and 1,2 ethylene dichloride is 3: 1 in described ethanol-1,2 dichloroethane solution.
4, the method for claim 1 is characterized in that, the sweep velocity of described differentiated pulse voltammetry is 10～50mV/s, and pulse height is 50mV.
5, the method for claim 1 is characterized in that, described differentiated pulse volt-ampere detects and carries out in nitrogen atmosphere, and described sample liquid is used N before carrying out the experiment of differentiated pulse volt-ampere
2Purifying.
6, the method for claim 1, it is characterized in that, the polypyrrole modifying electrode of described doping p-toluenesulfonic acid is made by following method: with platinum electrode as base electrode, place the acetonitrile solution of pyrrole monomer and p-toluenesulfonic acid, between the current potential of 0.8～1.0V, utilize electrochemical workstation to adopt constant potential method polymerization 80～120s.
7, method as claimed in claim 6, the concentration that it is characterized in that pyrrole monomer in the described acetonitrile solution is 0.02mol/L, the concentration of p-toluenesulfonic acid is 0.02mol/L.
8, method as claimed in claim 6 is characterized in that, the operating characteristic of described electrochemical workstation is: three electrode work systems, contrast electrode are Ag/AgCl, and auxiliary electrode is a platinum electrode, and working electrode is a platinum electrode.
9, a kind of electrode of measuring content of vitamin E, it is the polypyrrole modifying electrode of doping p-toluenesulfonic acid.
10, as the preparation method of electrode as described in the claim 9, it comprises: with platinum electrode as base electrode, place the acetonitrile solution of pyrrole monomer and p-toluenesulfonic acid, between the current potential of 0.8～1.0V, utilize electrochemical workstation to adopt constant potential method polymerization 80～120s.
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|---|---|---|---|---|
| CN101893595A (en) * | 2010-06-25 | 2010-11-24 | 中国农业大学 | Chemically modified electrode, preparation thereof and method for rapid determination of acid value of plant oil |
| CN102192944A (en) * | 2011-03-23 | 2011-09-21 | 天津市兰标电子科技发展有限公司 | Linear scanning stripping voltammetry method for detecting content of vitamins in blood sample |
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| CN102507809A (en) * | 2011-11-30 | 2012-06-20 | 河北科星药业有限公司 | Method for determining content of vitamin E in sodium selenite vitamin E injection |
| CN104297242A (en) * | 2014-11-03 | 2015-01-21 | 北京市药品检验所 | Method for rapidly detecting vitamin E or vitamin E-containing preparation |
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| CN101893595A (en) * | 2010-06-25 | 2010-11-24 | 中国农业大学 | Chemically modified electrode, preparation thereof and method for rapid determination of acid value of plant oil |
| CN102192932B (en) * | 2011-03-23 | 2013-10-02 | 天津市兰标电子科技发展有限公司 | Normal pulse stripping method for detecting content of vitamin in blood samples |
| CN102207484A (en) * | 2011-03-23 | 2011-10-05 | 天津市兰标电子科技发展有限公司 | Cyclic square wave voltammetry for detecting vitamin content in blood sample |
| CN102192946B (en) * | 2011-03-23 | 2013-07-03 | 天津市兰标电子科技发展有限公司 | Square wave volt-ampere method for detecting vitamin content in blood samples |
| CN102192945A (en) * | 2011-03-23 | 2011-09-21 | 天津市兰标电子科技发展有限公司 | Osteryoung square wave stripping voltammetry for detecting content of vitamins in blood sample |
| CN102221574B (en) * | 2011-03-23 | 2013-07-03 | 天津市兰标电子科技发展有限公司 | Linear sweep voltammetry for detecting vitamin content of blood sample |
| CN102221575A (en) * | 2011-03-23 | 2011-10-19 | 天津市兰标电子科技发展有限公司 | Differential pulse stripping voltammetry for detecting vitamin content in blood sample |
| CN102221574A (en) * | 2011-03-23 | 2011-10-19 | 天津市兰标电子科技发展有限公司 | Linear sweep voltammetry for detecting vitamin content of blood sample |
| CN102192944B (en) * | 2011-03-23 | 2013-10-02 | 天津市兰标电子科技发展有限公司 | Linear scanning stripping voltammetry method for detecting content of vitamins in blood sample |
| CN102192946A (en) * | 2011-03-23 | 2011-09-21 | 天津市兰标电子科技发展有限公司 | Square wave volt-ampere method for detecting vitamin content in blood samples |
| CN102192932A (en) * | 2011-03-23 | 2011-09-21 | 天津市兰标电子科技发展有限公司 | Normal pulse stripping method for detecting content of vitamin in blood samples |
| CN102207484B (en) * | 2011-03-23 | 2014-06-04 | 天津市兰标电子科技发展有限公司 | Cyclic square wave voltammetry for detecting vitamin content in blood sample |
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| CN104764838A (en) * | 2014-01-07 | 2015-07-08 | 天津优标技术检测服务有限公司 | Method for concurrently determining vitamin A content and vitamin E content in health product |
| CN104297242A (en) * | 2014-11-03 | 2015-01-21 | 北京市药品检验所 | Method for rapidly detecting vitamin E or vitamin E-containing preparation |
| CN104297242B (en) * | 2014-11-03 | 2016-09-07 | 北京市药品检验所 | Quickly detection vitamin E or the method containing Vitamin E preparation |
| CN114894878A (en) * | 2022-05-24 | 2022-08-12 | 福州大学 | Method for measuring concentration of inhibitor in acidic copper plating solution |
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