CN108132284B - A kind of test method of electrochemical sensor - Google Patents
A kind of test method of electrochemical sensor Download PDFInfo
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- CN108132284B CN108132284B CN201711431382.3A CN201711431382A CN108132284B CN 108132284 B CN108132284 B CN 108132284B CN 201711431382 A CN201711431382 A CN 201711431382A CN 108132284 B CN108132284 B CN 108132284B
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Abstract
The present invention relates to technical field of electrochemical detection, in particular to a kind of test method of electrochemical sensor.When test method provided by the invention can be accurately judged to detect sample to be tested using electrochemical sensor, whether sample introduction is correct, and, the deviation of detected value and true value can be significantly reduced, the result measured is more accurate, reliable, efficiently solves the problems, such as existing detection method detection inaccuracy.
Description
Technical field
The present invention relates to technical field of electrochemical detection more particularly to a kind of test methods of electrochemical sensor.
Background technique
Electrochemical sensor (strip) be used to detect or measure in fluid sample all the time with the presence or absence of certain substance,
Or the concentration level of the substance, testing result are used as the foundation of health reference or medical diagnosis on disease.Electrochemical sensor includes
Reagent mixture and one or more electrodes, wherein the reagent mixture includes at least one electron transfer agent (also referred to as " electricity
Sub- medium ") and the biocatalysis protein different because of analyte (such as specific enzyme).Sensors with auxiliary electrode relies on electronic media and electricity
Electronics transfer between pole surface, and played a role by measuring electrochemical redox reaction.In electrochemica biological sensor
In system or device, electron transfer reaction is monitored using with the concentration dependent electric signal for being tested analyte in fluid sample.Benefit
Started with such electrochemical sensor detection body fluid such as the analyte in blood or blood source product, tear, urine and saliva
It is taken seriously, and in some cases, it is most important to the health for maintaining certain individuals.In health care technology field, need pair
Specific components in human body fluid are monitored, especially diabetic.
In existing electrochemical sensor test method, due to the difference of input mode, it is possible that due to sample introduction side
To difference cause individual electrode that can not inhale sample completely, or scattered due to sample underdosage, sample surface and make electrode not
Can be completely filled, or due to multiple batch sampling, eventually lead to electrode detection less than or the signal inaccuracy that detects, such as
Three electrodic electron sensors, due to input mode, dosage difference, sample itself, certain differences of instrument electronics
Feature or detection environment temperature etc. factor, cause two working electrodes that can successively be chemically reacted, and chemically react and generate
Current value be first big after small.The current signal detected in this way, I1 is relatively small since first kinetic current tends to be steady, I2 by
In rear kinetic current still in relatively high level, so two current signals detected is of different sizes, wherein I1 pairs
The calculating concentration answered is relatively close together actual concentration, and the corresponding calculating concentration of I2 and actual concentration deviation are larger, finally lead
Causing detectable concentration, there are relatively large deviations with actual concentration, influence the accuracy of testing result.
Summary of the invention
The present invention provides a kind of test method of electrochemical sensor, this method can significantly reduce in sample to be tested to
The measured value of substance and the deviation of true value are surveyed, so that testing result is closer with true value, testing result is more acurrate.
The present invention provides a kind of test methods of electrochemical sensor, comprising:
Sample to be tested is added to electrochemical sensor, the reagent on sample to be tested and two working electrodes of electrochemical sensor
Occur to chemically react and generate two electric signals, respectively corresponds and obtain two current value I1And I2, according to I1/I2Ratio in judgement
Sample introduction situation；
The judgment method includes the following steps:
It is arranged preset parameter range (A, B), as A≤I1/I2When≤B, show that sample introduction is correct.
Preferably, the value that the value of the A is 0.5~0.8, B is 1.1~1.2.
The present invention provides a kind of test method of electrochemical sensor, comprising:
Sample to be tested is added to electrochemical sensor, the reagent on sample to be tested and two working electrodes of electrochemical sensor
Occur to chemically react and generate two electric signals, respectively corresponds and obtain two current value I1And I2, according to I1、I2Calculating current value
I obtains the concentration of test substance in sample to be tested；
Wherein, the calculation method of current value I are as follows:
I=aI1+bI2, 0.5≤a≤1,0≤b≤0.5.
Wherein, a, b are preferably a+b=1；Further preferably a=0.5, b=0.5.
Preferably, in the test method of electrochemical sensor provided by the invention, the calculation method of the current value I
Are as follows:
It is arranged preset parameter range (M, N), works as I1/I2≤ M or I1/I2When >=N, I=aI1+bI2；
As M < I1/I2When < N, I=aI1+kbI2, wherein k is penalty coefficient, k≤1；
Wherein, 0.5≤a≤1,0≤b≤0.5, a+b=1.
Preferably, penalty coefficient k are as follows: 0.80≤k≤0.98, more preferably 0.96.
Preferably, it is 1.0~1.2 that M value, which is 0.7~0.9, N value,.
In the test method of electrochemical sensor provided by the invention, I1/I2≤ M or I1/I2When >=N, a=1, b=0, I
=I1。
Test method of the present invention can be used for testing in blood, blood source product, tear, urine or saliva equal samples
The substances such as cholesterol, protein, glucose.
The test method of three-electrode electrochemical sensor provided by the invention, wherein three-electrode electrochemical sensor, including
Three electrodes, i.e. first electrode, second electrode and third electrode.
Preferably, three electrodes are triangularly arranged, first electrode and second electrode are located above and symmetrically arrange
Column, third electrode are located below and are located on the symmetry axis of first electrode and second electrode；Under the premise of manufacturing process allows,
Third electrode is as close as possible to first electrode and second electrode；Meanwhile on vertical direction (sample introduction direction), the top of third electrode
Edge must not be more than the lower edge of first electrode and second electrode upwards.
There are following several situations for the arrangement mode of three electrodes:
First sensor is the first working electrode W1, and second sensor is the second working electrode W2, and third electrode is as ginseng
Than electrode R, as shown in Fig. 1 (A)；
In first sensor and second sensor, one is used as working electrode W1, another is as reference electrode R, third
Electrode is as working electrode (or auxiliary electrode) W2, as shown in Fig. 1 (B)~(C).
The present invention provides a kind of test method of electrochemical sensor, this method can significantly reduce in sample to be tested to
The measured value of substance and the deviation of true value are surveyed, so that testing result is closer with true value, testing result is more acurrate.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described.
Fig. 1 is the electrode arranging structure schematic diagram of electrochemical sensor, and wherein W1, W2 show that working electrode, R show reference electricity
Pole.
Specific embodiment
The invention discloses a kind of test method of electrochemical sensor, those skilled in the art can be used for reference in this paper
Hold, is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to those skilled in the art
For be it will be apparent that they are considered as being included in the present invention.Method and application of the invention, which has passed through, preferably to be implemented
Example is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to method described herein and
Using being modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
To the explanation of the disclosed embodiments, enable those skilled in the art to implement or use the present invention.To this
A variety of modifications of a little embodiments will be readily apparent to those skilled in the art, as defined herein general
Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention will not
It can be intended to be limited to the embodiments shown herein, and be to fit to consistent with the principles and novel features disclosed in this article
Widest scope.
Below with reference to embodiment, the present invention is further explained:
The test method of the present invention of embodiment 1
Sample to be tested is added, sample to be tested enters sensor from the top sample entrance port of sensor, first sample filling the
One electrode and second electrode (W1 and R), make to constitute current loop between first electrode and second electrode, connect at this time with sensor
Instrument inside it under the driving of boot-strap circuit can Auto Power On, on sample to be tested and two working electrodes of electrochemical sensor
Reagent chemically react, instrument start in timing 15 to 30 seconds read obtain two current signals, respectively correspond I1With
I2, according to I1/I2Ratio in judgement sample introduction situation.Judgment method includes the following steps: setting preset parameter range (A, B), works as A
≤I1/I2When≤B, show that sample introduction is correct.
The test method of the present invention of embodiment 2
Sample to be tested is added, sample to be tested enters sensor from the top sample entrance port of sensor, first sample filling the
One electrode and second electrode (W1 and R), make to constitute current loop between first electrode and second electrode, connect at this time with sensor
Instrument inside it under the driving of boot-strap circuit can Auto Power On, on sample to be tested and two working electrodes of electrochemical sensor
Reagent chemically react, instrument start in timing 15 to 30 seconds read obtain two current signals, respectively correspond acquisition
Two current value I1And I2, according to I1、I2Calculating current value I obtains the concentration of test substance in sample to be tested；
Wherein, the calculation method of current value I are as follows: I=a1I1+b1I2, 0.5≤a≤1,0≤b≤0.5.
The test method of the present invention of embodiment 3
Sample to be tested is added, sample to be tested enters sensor from the top sample entrance port of sensor, first sample filling the
One electrode and second electrode (W1 and R), make to constitute current loop between first electrode and second electrode, connect at this time with sensor
Instrument inside it under the driving of boot-strap circuit can Auto Power On, on sample to be tested and two working electrodes of electrochemical sensor
Reagent chemically react, instrument start in timing 15 to 30 seconds read obtain two current signals, respectively correspond acquisition
Two current value I1And I2, according to I1、I2Calculating current value I obtains the concentration of test substance in sample to be tested；
Wherein, the calculation method of current value I are as follows:
It is arranged preset parameter range (M, N), M value is that 0.7~0.9, N value is 1.0~1.2；
As M < I1/I2When < N, I=aI1+kbI2, wherein A=0.5, b=0.5, k=0.97
Work as I1/I2≤ M or I1/I2When >=N, I=aI1+bI2, wherein a=1, b=0.
Embodiment 4
The sample introduction situation of 16 blood samples is tested using the test method of embodiment 1, wherein sample 1~8 is pressed
According to the test request sample introduction of biosensor test strip, sample 9~16 by manual operation make sample introduction be unsatisfactory for normally detect want
It asks.It the results are shown in Table 1.
Table 1
Note: I1 is the current value being calculated on electrode W1, and I2 is the current value being calculated on electrode W2.
The results show that can be accurately judged to using test method of the present invention whether correct in sample introduction.
Embodiment 5
The blood-sugar content in three samples to be tested S1, S2, S3 is tested using the test method of embodiment 3, each
Sample is repeated 8 times, and testing result is shown in Table 2.
The testing result of blood-sugar content in 2 blood sample of table
Note: C0For the actual concentration value of sample, detect to obtain by Biochemical Analyzer；I1For the current value on electrode W1, I2
For the current value on electrode W2, I is to pass through I1And I2The current value being calculated；C1It is the concentration value being calculated according to I1, C2
The concentration value being calculated according to I2, C be calculated by I cross value；" deviation 1 " is C1With C0The deviation compared, " partially
Poor 2 " be C2With C0Deviation, " deviation " is C and C0The deviation compared.
The results show that the concentration value C and true value C of blood glucose in blood sample to be measured are calculated using test method of the present invention0
Deviation between 0.1~2.9%, hence it is evident that reduce the deviation (deviation between the detected value and true value of existing detection method
1, deviation 2), testing result is more true, reliable.
Using the test method of the embodiment of the present invention 1 to other substances such as cholesterol, protein etc. in blood, in urine
The substances such as glucose detected, it is also evident that reduce the deviation between detected value and true value, testing result is true,
Reliably.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (1)
1. a kind of test method of electrochemical sensor characterized by comprising
Sample to be tested is added to electrochemical sensor, the reagent on sample to be tested and two working electrodes of electrochemical sensor occurs
Two electric signals are chemically reacted and generated, respectively corresponds and obtains two current value I1And I2, according to I1、I2Calculating current value I, is obtained
Obtain the concentration of test substance in sample to be tested；
Wherein, the calculation method of current value I are as follows:
It is arranged preset parameter range (M, N), M value is that 0.7~0.9, N value is 1.0~1.2；
Work as I1/I2≤ M or I1/I2When >=N, I=aI1+bI2, wherein a=1, b=0；
As M < I1/I2When < N, I=aI1+kbI2, wherein k is penalty coefficient, A=0.5, b=0.5, k=0.97.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1426535A (en) * | 2000-03-08 | 2003-06-25 | 因弗内斯医疗有限公司 | Measurement of substances in liquids |
| CN1558224A (en) * | 2003-02-11 | 2004-12-29 | �����ι�˾ | Methods of determining the concentration of an analyte in a fluid test sample |
| CN1589400A (en) * | 2001-11-20 | 2005-03-02 | 爱科来株式会社 | Fail judging method and analyzer |
| CN1668916A (en) * | 2002-09-30 | 2005-09-14 | 因福皮亚有限公司 | Apparatus and method for measuring reaction result of samples on biosensor |
| CN1902477A (en) * | 2003-10-31 | 2007-01-24 | 生命扫描苏格兰有限公司 | Method of reducing interferences in an electrochemical sensor using two different applied potentials |
| CN107121478A (en) * | 2017-05-01 | 2017-09-01 | 台州亿联健医疗科技有限公司 | A kind of biology sensor that can detect that sample is not enough, method |
| CN107209140A (en) * | 2014-11-06 | 2017-09-26 | 因赛生物计量有限公司 | The application method of test device and test device |
-
2017
- 2017-12-26 CN CN201711431382.3A patent/CN108132284B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1426535A (en) * | 2000-03-08 | 2003-06-25 | 因弗内斯医疗有限公司 | Measurement of substances in liquids |
| CN1589400A (en) * | 2001-11-20 | 2005-03-02 | 爱科来株式会社 | Fail judging method and analyzer |
| CN1668916A (en) * | 2002-09-30 | 2005-09-14 | 因福皮亚有限公司 | Apparatus and method for measuring reaction result of samples on biosensor |
| CN1558224A (en) * | 2003-02-11 | 2004-12-29 | �����ι�˾ | Methods of determining the concentration of an analyte in a fluid test sample |
| CN1902477A (en) * | 2003-10-31 | 2007-01-24 | 生命扫描苏格兰有限公司 | Method of reducing interferences in an electrochemical sensor using two different applied potentials |
| CN107209140A (en) * | 2014-11-06 | 2017-09-26 | 因赛生物计量有限公司 | The application method of test device and test device |
| CN107121478A (en) * | 2017-05-01 | 2017-09-01 | 台州亿联健医疗科技有限公司 | A kind of biology sensor that can detect that sample is not enough, method |
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