CN102178974A - Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application - Google Patents
Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application Download PDFInfo
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Abstract
The invention relates to a bacterial cellulose-based antibacterial dry film for acute trauma, which comprises the following components: a bacterial cellulose dry film and an antibacterial agent, and the drug loading the bacterial cellulose-based antibacterial dry film is 0.3-6.0 mg/cm2; the preparation method comprises the following steps: cutting a bacterial cellulose wet film into film sheets, drying, dipping the sheets in a pre-prepared inorganic antibacterial agent solution, and drying for the second time to obtain a product. The bacterial cellulose-based antibacterial dry film of the invention has the advantages of light and soft texture, good biocompatibility, high hygroscopicity, good permeability, lasting antibacterial property and the like; the preparation method is simple and practical, and is low in cost; during practical application, the bacterial cellulose-based antibacterial dry film can be tailored into any shape, does not adhere to or irritate skin, can be rapidly degraded in the environment after being discarded, and can be used as a green and environment-friendly functional antibacterial dressing.
Description
Technical field
The invention belongs to Bacterial cellulose base antibacterial film and its production and application field, particularly a kind of bacterial cellulose (BC) based antibacterial dry film and preparation method and application that is used for acute injury.
Background technology
Bacterial cellulose (Bacterial Cellulose, BC) be a kind of by the synthetic native cellulose of microorganism, from chemical molecular formula, BC and common plant cellulose do not have any difference, but give its high mechanical strength and biocompatibility because of physics, the chemical constitution of uniqueness.The BC that is shaped is made of the fiber of highly crystalline body and highly single guiding banded superfine fibre, intricately is intertwined and forms unique tridimensional network, the unique tridimensional network of BC inside has a lot " duct ", high ventilation, water permeability are arranged, can absorb 60～700 times of moisture content to its dry weight, these moisture are that the form with Free water exists.Use BC pastes material as wound can absorb wound blood and tissue fluid rapidly, prevents that wound infection from suppurating, and can provide moistening environment to promote wound healing and ease the pain again near the tissue regeneration the chronic wounds.Simultaneously cellulose not can with the wound adhesion, can not cause the secondary injury, do not have residual when peeling off yet.
At present report mainly is based on wet film with Bacterial cellulose film preparation wound dressing, and mostly is applied to chronic trauma, such as decubital ulcer, scald, cold injury etc.A kind of trade name is gone on the market the BC wound dressing of Biofill.It is actually with the regulation and control of the fiber content in the wet BC film extremely to a certain degree, makes the BC film can absorb wound tissue's liquid and prevents suppurative infection, can promote the different bacteria cellulose material of a kind of moisture content of wound healing for chronic wounds provides moistening environment again.Studies show that this BC dressing can more effectively promote wound healing in the application facet of chronic trauma than the dressing of other materials.Another BC wound dressing that is called Xcell also is used to promote the healing of chronic trauma, equally also shows good therapeutic effect.But above these BC dressing itself do not have anti-microbial property prevents wound infection.Xylos company releases the improvement BC dressing of a kind of antibiotic Xcell by name on the basis of original product Xcell, and goes on the market in the U.S. in 2003.Simultaneously also there are some researchs to give BC anti-microbial property by antibacterial such as composite nano silver or chitosan on BC.
But more frequent acute injury aspect takes place in daily life, and the application of BC dressing and research are then seldom.And be used for aspect the acute injury, the application of BC wet film is subjected to bigger restriction, than dry film in various degree decline is arranged all as fluid absorbents such as permeability, blood and tissue fluids.Be compared to wet film simultaneously, carrying of dry film is more convenient, and the comfort level of acute wounds also is better than wet film.At present, do not see the research and development report of the bacterial cellulose (BC) based antibacterial dry film that is used for acute injury both at home and abroad as yet.
Summary of the invention
Technical problem to be solved by this invention provides a kind of bacterial cellulose (BC) based antibacterial dry film and preparation method and application that is used for acute injury, advantages such as the antibiotic dry film of this BC has the light softness of quality, good biocompatibility, hygroscopicity height, good permeability, and antibiotic property is lasting; And preparation method is simple and easy to do, and is with low cost, can be used as a kind of functional antibiosis dressing of environmental protection, has a good application prospect.
A kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury of the present invention, its component comprises: Bacterial cellulose dry film and antibacterial, the drug loading of bacterial cellulose (BC) based antibacterial dry film are 0.3～6.0mg/cm
2Mg/cm
2Wherein, described antibacterial is natural organic antibacterial agent, animal sources natural antibacterial agent or vegetable source natural antibacterial.
The drug loading of described bacterial cellulose (BC) based antibacterial dry film is 0.5～5.7mg/cm
2
Described natural organic antibacterial agent is selected from one or more the mixture in lactic acid, epsilon-polylysine, nisin, chitosan and the derivant thereof; The animal sources natural antibacterial agent is selected from one or more the mixture in amino acids antibacterial, the native peptides class antibacterial; The vegetable source natural antibacterial is selected from one or more the mixture in Herba Andrographis, Bulbus Allii, Rhizoma Fagopyri Dibotryis, Ramulus Et Folium Picrasmae, Rhizoma Coptidis, berberine, Herba Houttuyniae, the houttuynine sodium bisulfite.
The amino acids antibacterial is a cysteine in the described animal sources natural antibacterial agent; Native peptides class antibacterial is selected from one or more the mixture in lysozyme, the lactoperoxidase.
Preferred antibacterial is chitosan, cysteine or berberine.
Described bacterial cellulose (BC) based antibacterial dry film can absorb the moisture content of 25～30 times of self dry weights.
Described bacterial cellulose (BC) based antibacterial dry film can continue delivery of antimicrobials 24～96 hours in the time of 37 ℃; Can reach bacteriostasis rate more than 98.7～99.8% to gram positive bacteria and negative bacterium.
A kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury of the present invention comprises:
(1) processing of BC film
Bacterial cellulose BC wet film is cut into diaphragm, through pretreatment or standby after need not to handle;
(2) preparation of antimicrobial
Antibacterial is made into the antimicrobial that concentration is 0.5wt%～5wt%; Wherein, described antibacterial is natural organic antibacterial agent, animal sources natural antibacterial agent or vegetable source natural antibacterial;
(3) preparation of the antibiotic dry film of BC
The BC diaphragm of step (1) preparation be impregnated in above-mentioned antimicrobial 24～30h, and drying makes the antibiotic dry film of BC.
Preferably, the natural organic antibacterial agent in the described step (2) is dissolved in 5% the acetic acid solution, is made into the antimicrobial that concentration is 1.0wt%～3.0wt%; The animal sources natural antibacterial agent is dissolved in the deionized water, is made into the antimicrobial that concentration is 1.0wt%～3.0wt%; Or the vegetable source natural antibacterial is dissolved in the deionized water, be made into the antimicrobial that concentration is 1.0wt%～3.0wt%.
Natural organic antibacterial agent in the described step (2) is selected from one or more the mixture in lactic acid, epsilon-polylysine, nisin, chitosan and the derivant thereof; The animal sources natural antibacterial agent is selected from one or more the mixture in amino acids antibacterial, the native peptides class antibacterial; The vegetable source natural antibacterial is selected from one or more the mixture in Herba Andrographis, Bulbus Allii, Rhizoma Fagopyri Dibotryis, Ramulus Et Folium Picrasmae, Rhizoma Coptidis, berberine, Herba Houttuyniae, the houttuynine sodium bisulfite.
The alternative method of described step (3) is for directly coating antimicrobial the BC membrane surface of step (1) preparation, and drying makes the antibiotic dry film of BC again.
Pretreatment in the described step (1) comprises adopts rotary evaporation, squeezing or exsiccant method to remove the moisture of BC diaphragm 50%-100%.
Drying in described step and (3) is normal temperature drying, vacuum drying or lyophilization.
A kind of application that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury at preparation acute injury medical material of the present invention.
Beneficial effect
Advantages such as (1) the antibiotic dry film of BC of the present invention has the light softness of quality, good biocompatibility, hygroscopicity height, good permeability, and antibiotic property is lasting; And preparation method is simple and easy to do, and is with low cost, but suitability for industrialized production;
(2) in actual application, can be cut into different shape arbitrarily according to the situation and the characteristics of wound, adhesion is chafe not also, safety and environmental protection, abandon the back and in environment, can degrade rapidly, can be used as a kind of functional antibiosis dressing of well behaved and environmental protection.
Description of drawings
Fig. 1 is the inhibitory action figure of the BC antibacterial film of 1.5% chitosan solution preparation to three kinds of common bacterias for concentration;
A is the bacteriostatic experiment to staphylococcus aureus; B is the bacteriostatic experiment to bacillus subtilis; C is to colibacillary bacteriostatic experiment;
Fig. 2 is the inhibitory action figure of the BC antibacterial film of 1.5% cysteine formulations prepared from solutions to three kinds of common bacterias for concentration;
A is to colibacillary bacteriostatic experiment; B is the bacteriostatic experiment to staphylococcus aureus; C is the bacteriostatic experiment to bacillus subtilis;
Fig. 3 is the inhibitory action figure of the BC antibacterial film of 1.5% berberine formulations prepared from solutions to three kinds of common bacterias for concentration;
A is to colibacillary bacteriostatic experiment; B is the bacteriostatic experiment to staphylococcus aureus; C is the bacteriostatic experiment to bacillus subtilis.
Fig. 4 is that the BC antibacterial film of 1.0% lactic acid solution preparation is to colibacillary inhibitory action figure for concentration.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
Preparation concentration is the chitosan solution of 1.5wt%;
(3) preparation of the antibiotic dry film of BC
Is in 1.5% the chitosan solution behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization obtains the antibiotic dry film of BC once more.
Dull and stereotyped diffusion method is measured the bacteriostasis of anti-biotic material
Test strain: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1L, pH 7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH 7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH 7; If solid medium then adds the agar of 20g.
Experimental technique
Preparation fluid medium and solid plate culture medium are inoculated into escherichia coli, staphylococcus aureus, bacillus subtilis the liquid seed culture medium from the inclined-plane seed then, and 37 ℃ of constant temperature culture got seed liquor in 12 hours; Draw the 0.1mL seed liquor to the solid plate culture medium, coating evenly; The BC antibacterial film for preparing is tiled in dull and stereotyped central authorities, and 37 ℃ of constant temperature are inverted and were cultivated 24 hours, and the footpath of measuring inhibition zone is poor, the results are shown in Figure 1.
Experiment shows: the antibiotic dry film of BC all has fungistatic effect preferably to three kinds of common bacterias.
Embodiment 2
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby behind the moisture content of squeezing removal 70-80%;
(2) preparation of antimicrobial
Preparation concentration is the lactic acid solution of 1wt%;
(3) preparation of the antibiotic dry film of BC
Is in the lactic acid solution of 1wt% behind the 24h with the BC film immersion after the squeezing in 25mL concentration, and lyophilization obtains antibiotic dry film once more.
The water absorbing properties test of the anti-film of BC
After the freeze dried BC anti-biotic material of secondary weighed, be immersed in 24h in the deionized water, blot the surface then and swim, weigh.The expansion rate of BC antibacterial film is calculated according to following formula: expansion rate=(W
s-W
d)/W
d, W wherein
sBe the weight in wet base after the BC film expands, W
dBe the original dry weight of BC film, experimental result sees Table 1.
The expansion rate of BC film after the lyophilizing of table 1 secondary
Experimental result shows: diameter is the BC film of 15mm is about own dry weight through its absorbability after the secondary lyophilizing 26.20 times.
Embodiment 3
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby;
(2) preparation of antimicrobial
Preparation concentration is the cysteine solution of 1.5wt%;
(3) preparation of the antibiotic dry film of BC
Is in 1.5% the cysteine solution behind the 24h with wet BC film direct impregnation in 25mL concentration, obtains the antibiotic dry film of BC through lyophilization.
Dull and stereotyped diffusion method is measured the bacteriostasis of anti-biotic material
Test strain: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1L, pH 7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH 7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH 7; If solid medium then adds the agar of 20g.
Experimental technique
Preparation fluid medium and solid plate culture medium are inoculated into escherichia coli, staphylococcus aureus, bacillus subtilis the liquid seed culture medium from the inclined-plane seed then, and 37 ℃ of constant temperature culture got seed liquor in 12 hours; Draw the 0.1mL seed liquor to the solid plate culture medium, coating evenly; The BC antibacterial film for preparing is tiled in dull and stereotyped central authorities, and 37 ℃ of constant temperature are inverted and were cultivated 24 hours, and the footpath of measuring inhibition zone is poor, the results are shown in Figure 2.
Experiment shows: the antibiotic dry film of BC all has fungistatic effect preferably to three kinds of common bacterias.
Embodiment 4
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
Preparation concentration is the lysozyme soln of 2.5wt%;
(3) preparation of the antibiotic dry film of BC
Is in the lysozyme soln of 2.5wt% behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization obtains antibiotic dry film once more.
The water absorbing properties test of the anti-film of BC
After the freeze dried BC anti-biotic material of secondary weighed, be immersed in 24h in the deionized water, blot the surface then and swim, weigh.The expansion rate of BC antibacterial film is calculated according to following formula: expansion rate=(W
s-W
d)/W
d, W wherein
sBe the weight in wet base after the BC film expands, W
dBe the original dry weight of BC film, experimental result sees Table 2.
The expansion rate of BC film after the lyophilizing of table 2 secondary
Experimental result shows: diameter is the BC film of 15mm is about own dry weight through its absorbability after the secondary lyophilizing 26.20 times.
Embodiment 5
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
Preparation concentration is the berberine solution of 1.5wt%;
(3) preparation of the antibiotic dry film of BC
Is in 1.5% the berberine solution behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization or vacuum drying obtain the antibiotic dry film of BC once more.
Dull and stereotyped diffusion method is measured the bacteriostasis of anti-biotic material
Test strain: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1L, pH 7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH 7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH 7; If solid medium then adds the agar of 20g.
Experimental technique
Preparation fluid medium and solid plate culture medium are inoculated into escherichia coli, staphylococcus aureus, bacillus subtilis the liquid seed culture medium from the inclined-plane seed then, and 37 ℃ of constant temperature culture got seed liquor in 12 hours; Draw the 0.1mL seed liquor to the solid plate culture medium, coating evenly; The BC antibacterial film for preparing is tiled in dull and stereotyped central authorities, and 37 ℃ of constant temperature are inverted and were cultivated 24 hours, and the footpath of measuring inhibition zone is poor, the results are shown in Figure 3.
Experiment shows: the antibiotic dry film of BC all has fungistatic effect preferably to three kinds of common bacterias.
Embodiment 6
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
Preparation concentration is the houttuynine sodium bisulfite solution of 2.5wt%;
(3) preparation of the antibiotic dry film of BC
Is in the houttuynine sodium bisulfite solution of 2.5wt% behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization or normal temperature drying obtain antibiotic dry film once more.
The water absorbing properties test of the anti-film of BC
After the freeze dried BC anti-biotic material of secondary weighed, be immersed in 24h in the deionized water, blot the surface then and swim, weigh.The expansion rate of BC antibacterial film is calculated according to following formula: expansion rate=(W
s-W
d)/W
d, W wherein
sBe the weight in wet base after the BC film expands, W
dBe the original dry weight of BC film, experimental result sees Table 3.
The expansion rate of BC film after the lyophilizing of table 3 secondary
Experimental result shows: diameter is the BC film of 15mm is about own dry weight through its absorbability after the secondary lyophilizing 26.20 times.
Claims (12)
1. bacterial cellulose (BC) based antibacterial dry film that is used for acute injury, its component comprises: Bacterial cellulose dry film and antibacterial, the drug loading of bacterial cellulose (BC) based antibacterial dry film are 0.3～6.0mg/cm
2Wherein, described antibacterial is natural organic antibacterial agent, animal sources natural antibacterial agent or vegetable source natural antibacterial.
2. a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury according to claim 1 is characterized in that: the drug loading of described bacterial cellulose (BC) based antibacterial dry film is 0.5～5.7mg/cm
2
3. a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury according to claim 1 is characterized in that: described natural organic antibacterial agent is selected from one or more the mixture in lactic acid, epsilon-polylysine, nisin, chitosan and the derivant thereof; The animal sources natural antibacterial agent is selected from one or more the mixture in amino acids antibacterial, the native peptides class antibacterial; The vegetable source natural antibacterial is selected from one or more the mixture in Herba Andrographis, Bulbus Allii, Rhizoma Fagopyri Dibotryis, Ramulus Et Folium Picrasmae, Rhizoma Coptidis, berberine, Herba Houttuyniae, the houttuynine sodium bisulfite.
4. a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury according to claim 3 is characterized in that: the amino acids antibacterial is a cysteine in the described animal sources natural antibacterial agent; Native peptides class antibacterial is selected from one or more the mixture in lysozyme, the lactoperoxidase.
5. a kind of bacterial cellulose (BC) based antibacterial dry film according to claim 1 is characterized in that: described bacterial cellulose (BC) based antibacterial dry film can absorb the moisture content of 25～30 times of self dry weights.
6. preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury comprises:
(1) processing of BC film
Bacterial cellulose BC wet film is cut into diaphragm, through pretreatment or standby after need not to handle;
(2) preparation of antimicrobial
Antibacterial is made into the antimicrobial that concentration is 0.5wt%～5wt%; Wherein, described antibacterial is natural organic antibacterial agent, animal sources natural antibacterial agent or vegetable source natural antibacterial;
(3) preparation of the antibiotic dry film of BC
The BC diaphragm of step (1) preparation be impregnated in above-mentioned antimicrobial 24～30h, and drying makes the antibiotic dry film of BC.
7. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 6, it is characterized in that: the natural organic antibacterial agent in the described step (2) is dissolved in 5% the acetic acid solution, is made into the antimicrobial that concentration is 1.0wt%～3.0wt%; The animal sources natural antibacterial agent is dissolved in the deionized water, is made into the antimicrobial that concentration is 1.0wt%～3.0wt%; Or the vegetable source natural antibacterial is dissolved in the deionized water, be made into the antimicrobial that concentration is 1.0wt%～3.0wt%.
8. according to claim 6 or 7 described a kind of preparation methoies that are used for the bacterial cellulose (BC) based antibacterial dry film of acute injury, it is characterized in that: the natural organic antibacterial agent in the described step (2) is selected from one or more the mixture in lactic acid, epsilon-polylysine, nisin, chitosan and the derivant thereof; The animal sources natural antibacterial agent is selected from one or more the mixture in amino acids antibacterial, the native peptides class antibacterial; The vegetable source natural antibacterial is selected from one or more the mixture in Herba Andrographis, Bulbus Allii, Rhizoma Fagopyri Dibotryis, Ramulus Et Folium Picrasmae, Rhizoma Coptidis, berberine, Herba Houttuyniae, the houttuynine sodium bisulfite.
9. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 6, it is characterized in that: the alternative method of described step (3) is for directly coating antimicrobial the BC membrane surface of step (1) preparation, and drying makes the antibiotic dry film of BC again.
10. according to claim 6 or 9 described a kind of preparation methoies that are used for the bacterial cellulose (BC) based antibacterial dry film of acute injury, it is characterized in that: the pretreatment in the described step (1) comprises adopts rotary evaporation, squeezing or exsiccant method to remove the moisture of BC diaphragm 50%-100%.
11. according to claim 6 or 9 described a kind of preparation methoies that are used for the bacterial cellulose (BC) based antibacterial dry film of acute injury, it is characterized in that: the drying in the described step (3) is normal temperature drying, vacuum drying or lyophilization.
12. application that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury at preparation acute injury medical material.
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| CN201010022842.9 | 2010-01-15 | ||
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| CN2011100096373A Pending CN102091346A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application thereof |
| CN2011100096142A Pending CN102178973A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose based antibacterial dry film for treating acute injury and preparation method and application thereof |
| CN201110009673XA Pending CN102178974A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN102178973A (en) | 2011-09-14 |
| CN102058897A (en) | 2011-05-18 |
| CN102091346A (en) | 2011-06-15 |
| CN101745141A (en) | 2010-06-23 |
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