CN102091346A - Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application thereof - Google Patents
Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application thereof Download PDFInfo
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- CN102091346A CN102091346A CN2011100096373A CN201110009637A CN102091346A CN 102091346 A CN102091346 A CN 102091346A CN 2011100096373 A CN2011100096373 A CN 2011100096373A CN 201110009637 A CN201110009637 A CN 201110009637A CN 102091346 A CN102091346 A CN 102091346A
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Abstract
The invention relates to a bacterial cellulose-based antibacterial dry film for acute trauma, which comprises a bacterial cellulose-based dry film and an inorganic antibacterial agent. The drug loading quantity of the bacterial cellulose-based antibacterial dry film is 1.00*10<-5>-4.0mg/cm<2>. The preparation method of the bacterial cellulose-based antibacterial dry film comprises the following steps: cutting a bacterial cellulose-based wet film into a diaphragm; drying; then, soaking the diaphragm into an inorganic antibacterial agent solution which is prepared in advance; and drying for the second time to obtain the bacterial cellulose-based antibacterial dry film. The bacterial cellulose-based antibacterial dry film provided by the invention has the advantages of light and soft texture, good biocompatibility, high hygroscopicity, good air permeability, lasting antibacterial property and the like; and the preparation method is simple, convenient and easy to implement and is low in cost. In the practical application process, the bacterial cellulose-based antibacterial dry film can be cut into pieces in arbitrary shapes, can not adhere to the skin, can not irritate the skin, can be quickly degraded in the environment after being discarded, and can be used as an environment-friendly functional antibacterial dressing.
Description
Technical field
The invention belongs to Bacterial cellulose base antibacterial film and its production and application field, particularly a kind of bacterial cellulose (BC) based antibacterial dry film and preparation and application that is used for acute injury.
Background technology
Bacterial cellulose (Bacterial Cellulose, BC) be a kind of by the synthetic native cellulose of microorganism, from chemical molecular formula, BC and common plant cellulose do not have any difference, but give its high mechanical strength and biocompatibility because of physics, the chemical constitution of uniqueness.The BC that is shaped is made of the fiber of highly crystalline body and highly single guiding banded superfine fibre, intricately is intertwined and forms unique tridimensional network, the unique tridimensional network of BC inside has a lot " duct ", high ventilation, water permeability are arranged, can absorb 60～700 times of moisture content to its dry weight, these moisture are that the form with Free water exists.Use BC pastes material as wound can absorb wound blood and tissue fluid rapidly, prevents that wound infection from suppurating, and can provide moistening environment to promote wound healing and ease the pain again near the tissue regeneration the chronic wounds.Simultaneously cellulose not can with the wound adhesion, can not cause the secondary injury, do not have residual when peeling off yet.
At present report mainly is based on wet film with Bacterial cellulose film preparation wound dressing, and mostly is applied to chronic trauma, such as decubital ulcer, scald, cold injury etc.A kind of trade name is gone on the market the BC wound dressing of Biofill.It is actually with the regulation and control of the fiber content in the wet BC film extremely to a certain degree, makes the BC film can absorb wound tissue's liquid and prevents suppurative infection, can promote the different bacteria cellulose material of a kind of moisture content of wound healing for chronic wounds provides moistening environment again.Studies show that this BC dressing can more effectively promote wound healing in the application facet of chronic trauma than the dressing of other materials.Another BC wound dressing that is called Xcell also is used to promote the healing of chronic trauma, equally also shows good therapeutic effect.But above these BC dressing itself do not have anti-microbial property prevents wound infection.Xylos company releases the improvement BC dressing of a kind of antibiotic Xcell by name on the basis of original product Xcell, and goes on the market in the U.S. in 2003.Simultaneously also there are some researchs to give BC anti-microbial property by antibacterial such as composite nano silver or chitosan on BC.
But more frequent acute injury aspect takes place in daily life, and the application of BC dressing and research are then seldom.And be used for aspect the acute injury, the application of BC wet film is subjected to bigger restriction, than dry film in various degree decline is arranged all as fluid absorbents such as permeability, blood and tissue fluids.Be compared to wet film simultaneously, carrying of dry film is more convenient, and the comfort level of acute wounds also is better than wet film.At present, do not see the research and development report of the bacterial cellulose (BC) based antibacterial dry film that is used for acute injury both at home and abroad as yet.
Summary of the invention
Technical problem to be solved by this invention provides a kind of bacterial cellulose (BC) based antibacterial dry film and preparation and application that is used for acute injury, advantages such as the antibiotic dry film of this BC has the light softness of quality, good biocompatibility, hygroscopicity height, good permeability, and antibiotic property is lasting; And preparation method is simple and easy to do, and is with low cost, can be used as a kind of functional antibiosis dressing of environmental protection, has a good application prospect.
A kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury of the present invention, its component comprises: Bacterial cellulose dry film and inorganic antiseptic, the drug loading of bacterial cellulose (BC) based antibacterial dry film are 1.00 * 10
-5～4.0mg/cm
2
Described inorganic antiseptic is chloride antibacterial, peroxide antibacterial, contain iodine antibacterial or nano antibacterial agent.
Wherein, when selecting chloride antibacterial as inorganic antiseptic for use, the drug loading of bacterial cellulose (BC) based antibacterial dry film is 1.14 * 10
-5～3.41 * 10
-4Mg/cm
2
When selecting the peroxide antibacterial as inorganic antiseptic for use, the drug loading of bacterial cellulose (BC) based antibacterial dry film is 1.14 * 10
-5～5.68 * 10
-2Mg/cm
2
When selecting for use when containing the iodine antibacterial as inorganic antiseptic, the drug loading of bacterial cellulose (BC) based antibacterial dry film is 0.0852～3.4071mg/cm
2
Perhaps when selecting nano antibacterial agent as inorganic antiseptic for use, the drug loading of bacterial cellulose (BC) based antibacterial dry film is 0.0341～0.0568mg/cm
2
Described chloride antibacterial is selected from one or more the mixture in sodium hypochlorite, calcium hypochlorite or the Efficacious Disinfeitant; The peroxide antibacterial is selected from one or more the mixture in hydrogen peroxide, peracetic acid, the chlorine dioxide; Contain the iodine antibacterial and be selected from one or more mixture in iodine tincture, the povidone iodine; Nano antibacterial agent is selected from nano-TiO
2Granule, nano zine oxide, be loaded with Ag
+Nano material, be loaded with TiO
2Nano material in one or more mixture.
Preferred antibacterial is sodium hypochlorite, chlorine dioxide or iodine tincture.
Described bacterial cellulose (BC) based antibacterial dry film can absorb the moisture content of 25～30 times of self dry weights.
Described bacterial cellulose (BC) based antibacterial dry film can continue delivery of antimicrobials 24～96 hours in the time of 37 ℃; Can reach the bacteriostasis rate of 99.9 above % to Gram-positive and negative bacterium.
A kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury of the present invention comprises:
(1) processing of BC film
Bacterial cellulose BC wet film is cut into diaphragm, through pretreatment or standby after need not to handle;
(2) preparation of antimicrobial
Above-mentioned inorganic antiseptic is made into the antimicrobial that concentration is 0.01～5wt%;
(3) preparation of the antibiotic dry film of BC
The BC diaphragm of step (1) preparation be impregnated in above-mentioned antimicrobial 24～30h, and drying makes the antibiotic dry film of BC.
Inorganic antiseptic in the described step (2) is chloride antibacterial, peroxide antibacterial, contain iodine antibacterial or nano antibacterial agent;
Wherein, specifically comprise:
Chloride antibacterial is dissolved in the deionized water, is made into the antimicrobial that concentration is 0.1～3mg/L; Preferred concentration is the aqueous sodium hypochlorite solution of 2mg/L
The peroxide antibacterial is dissolved in the deionized water, is made into the antimicrobial that concentration is 0.1～500mg/L; Preferred concentration is the ClO 2 solution of 50mg/L;
To contain the iodine antibacterial and be dissolved in 75% ethanol, be made into concentration and be 0.1%～4% antimicrobial; Preferred concentration is 2% iodine tincture solution;
Or above-mentioned nano antibacterial agent is dissolved in the deionized water, be made into the antimicrobial that concentration is 0.3wt ‰～0.5wt ‰; Preferred concentration is the nanometer Ag solution of 0.3wt ‰.
The alternative method of described step (3) is for directly coating antimicrobial the BC membrane surface of step (1) preparation, and drying makes the antibiotic dry film of BC again.
Pretreatment in the described step (1) comprises adopts rotary evaporation, squeezing or exsiccant method to remove the moisture of BC diaphragm 50%-100%.
Drying in the described step (3) is normal temperature drying, vacuum drying or lyophilization.
A kind of application that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury at preparation acute injury medical material of the present invention.
Beneficial effect
Advantages such as (1) the antibiotic dry film of BC of the present invention has the light softness of quality, good biocompatibility, hygroscopicity height, good permeability, and antibiotic property is lasting; And preparation method is simple and easy to do, and is with low cost, but suitability for industrialized production;
(2) in actual application, can be cut into different shape arbitrarily according to the situation and the characteristics of wound, adhesion is chafe not also, safety and environmental protection, abandon the back and in environment, can degrade rapidly, can be used as a kind of functional antibiosis dressing of well behaved and environmental protection.
Description of drawings
Fig. 1 is that the BC antibacterial film of liquor natrii hypochloritis preparation of 2mg/L is to the inhibitory action figure of three kinds of common bacterias for concentration;
A is to colibacillary bacteriostatic experiment; B is the bacteriostatic experiment to staphylococcus aureus; C is the bacteriostatic experiment to bacillus subtilis;
Fig. 2 is that the BC antibacterial film of ClO 2 solution preparation of 50mg/L is to the inhibitory action figure of three kinds of common bacterias for concentration;
A is to colibacillary bacteriostatic experiment; B is the bacteriostatic experiment to staphylococcus aureus; C is the bacteriostatic experiment to bacillus subtilis;
Fig. 3 is the inhibitory action figure of the BC antibacterial film of 2% iodine tincture formulations prepared from solutions to three kinds of common bacterias for concentration;
A is to colibacillary bacteriostatic experiment; B is the bacteriostatic experiment to staphylococcus aureus; C is the bacteriostatic experiment to bacillus subtilis;
Fig. 4 is the inhibitory action figure of the BC antibacterial film of 0.3 ‰ nanometer Ag formulations prepared from solutions to three kinds of common bacterias for concentration;
A is to colibacillary bacteriostatic experiment; B is the bacteriostatic experiment to staphylococcus aureus; C is the bacteriostatic experiment to bacillus subtilis.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
Preparation concentration is the liquor natrii hypochloritis of 2mg/L;
(3) preparation of the antibiotic dry film of BC
Is among the liquor natrii hypochloritis of 2mg/L behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization obtains the antibiotic dry film of BC once more.
Dull and stereotyped diffusion method is measured the bacteriostasis of anti-biotic material
Test strain: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1 L, pH 7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH 7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH 7;
If solid medium then adds the agar of 20g.
Experimental technique
Preparation fluid medium and solid plate culture medium are inoculated into escherichia coli, staphylococcus aureus, bacillus subtilis the liquid seed culture medium from the inclined-plane seed then, and 37 ℃ of constant temperature culture got seed liquor in 12 hours; Draw the 0.1mL seed liquor to the solid plate culture medium, coating evenly; The BC antibacterial film for preparing is tiled in dull and stereotyped central authorities, and 37 ℃ of constant temperature are inverted and were cultivated 24 hours, and the footpath of measuring inhibition zone is poor, the results are shown in Figure 1.
Experiment shows: the antibiotic dry film of BC all has fungistatic effect preferably to three kinds of common bacterias.
Embodiment 2
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby behind the moisture content of squeezing removal 70-80%;
(2) preparation of antimicrobial
Preparation concentration is the ClO 2 solution of 50mg/L;
(3) preparation of the antibiotic dry film of BC
Is in the ClO 2 solution of 50mg/L behind the 24h with the BC film immersion after the squeezing in 25mL concentration, and lyophilization obtains the antibiotic dry film of BC once more.
Dull and stereotyped diffusion method is measured the bacteriostasis of anti-biotic material
Test strain: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1 L, pH 7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH 7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH 7;
If solid medium then adds the agar of 20g.
Experimental technique
Preparation fluid medium and solid plate culture medium are inoculated into escherichia coli, staphylococcus aureus, bacillus subtilis the liquid seed culture medium from the inclined-plane seed then, and 37 ℃ of constant temperature culture got seed liquor in 12 hours; Draw the 0.1mL seed liquor to the solid plate culture medium, coating evenly; The BC antibacterial film for preparing is tiled in dull and stereotyped central authorities, and 37 ℃ of constant temperature are inverted and were cultivated 24 hours, and the footpath of measuring inhibition zone is poor, the results are shown in Figure 2.
Experiment shows: the antibiotic dry film of BC all has fungistatic effect preferably to three kinds of common bacterias.
Embodiment 3
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby;
(2) preparation of antimicrobial
Preparation concentration is 2% iodine tincture solution;
(3) preparation of the antibiotic dry film of BC
Is in 2% the iodine tincture solution behind the 24h with wet BC film direct impregnation in 25mL concentration, and lyophilization obtains the antibiotic dry film of BC once more.
Dull and stereotyped diffusion method is measured the bacteriostasis of anti-biotic material
Test strain: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1 L, pH 7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH 7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH 7; If solid medium then adds the agar of 20g.
Experimental technique
Preparation fluid medium and solid plate culture medium are inoculated into escherichia coli, staphylococcus aureus, bacillus subtilis the liquid seed culture medium from the inclined-plane seed then, and 37 ℃ of constant temperature culture got seed liquor in 12 hours; Draw the 0.1mL seed liquor to the solid plate culture medium, coating evenly; The BC antibacterial film for preparing is tiled in dull and stereotyped central authorities, and 37 ℃ of constant temperature are inverted and were cultivated 24 hours, and the footpath of measuring inhibition zone is poor, the results are shown in Figure 3.
Experiment shows: the antibiotic dry film of BC all has fungistatic effect preferably to three kinds of common bacterias.
Embodiment 4
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
Preparation concentration is 1% iodophor solution;
(3) preparation of the antibiotic dry film of BC
Is in 1% the iodophor solution behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization obtains antibiotic dry film once more.
The water absorbing properties test of the anti-film of BC
After the freeze dried BC anti-biotic material of secondary weighed, be immersed in 24h in the deionized water, blot the surface then and swim, weigh.The expansion rate of BC antibacterial film is calculated according to following formula: expansion rate=(W
s-W
d)/W
d, W wherein
sBe the weight in wet base after the BC film expands, W
dBe the original dry weight of BC film, experimental result sees Table 1.
The expansion rate of BC film after the lyophilizing of table 1 secondary
Experimental result shows: diameter is the BC film of 15mm is about own dry weight through its absorbability after the secondary lyophilizing 26.20 times.
Embodiment 5
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
Preparation concentration is the nanometer Ag solution of 0.3wt ‰;
(3) preparation of the antibiotic dry film of BC
Is in 0.3 ‰ the nanometer Ag solution behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization or vacuum drying obtain the antibiotic dry film of BC once more.
Dull and stereotyped diffusion method is measured the bacteriostasis of anti-biotic material
Test strain: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1 L, pH 7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH 7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH 7;
If solid medium then adds the agar of 20g.
Experimental technique
Preparation fluid medium and solid plate culture medium are inoculated into escherichia coli, staphylococcus aureus, bacillus subtilis the liquid seed culture medium from the inclined-plane seed then, and 37 ℃ of constant temperature culture got seed liquor in 12 hours; Draw the 0.1mL seed liquor to the solid plate culture medium, coating evenly; The BC antibacterial film for preparing is tiled in dull and stereotyped central authorities, and 37 ℃ of constant temperature are inverted and were cultivated 24 hours, and the footpath of measuring inhibition zone is poor, the results are shown in Figure 4.
Experiment shows: the antibiotic dry film of BC all has fungistatic effect preferably to three kinds of common bacterias.
Embodiment 6
(1) processing of BC film
The BC film that makes is cut into the disk that diameter is 15mm, standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
Preparation concentration is the nano-TiO of 0.5wt ‰
2Solution;
(3) preparation of the antibiotic dry film of BC
With freeze dried BC film immersion is the nano-TiO of 0.5wt ‰ in 25mL concentration
2Behind the 24h, lyophilization or normal temperature drying obtain antibiotic dry film once more in the solution.
The water absorbing properties test of the anti-film of BC
After the freeze dried BC anti-biotic material of secondary weighed, be immersed in 24h in the deionized water, blot the surface then and swim, weigh.The expansion rate of BC antibacterial film is calculated according to following formula: expansion rate=(W
s-W
d)/W
d, W wherein
sBe the weight in wet base after the BC film expands, W
dBe the original dry weight of BC film, experimental result sees Table 2.
The expansion rate of BC film after the lyophilizing of table 2 secondary
Experimental result shows: diameter is the BC film of 15mm is about own dry weight through its absorbability after the secondary lyophilizing 26.20 times.
Claims (12)
1. bacterial cellulose (BC) based antibacterial dry film that is used for acute injury, its component comprises: Bacterial cellulose dry film and inorganic antiseptic, the drug loading of bacterial cellulose (BC) based antibacterial dry film are 1.00 * 10
-5～4.0mg/cm
2
2. a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury according to claim 1 is characterized in that: described inorganic antiseptic is chloride antibacterial, peroxide antibacterial, contain iodine antibacterial or nano antibacterial agent.
3. a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury according to claim 2, it is characterized in that: described bacterial cellulose (BC) based antibacterial dry film, its component comprises: Bacterial cellulose dry film and chloride antibacterial, the drug loading of bacterial cellulose (BC) based antibacterial dry film are 1.14 * 10
-5～3.41 * 10
-4Mg/cm
2
Described bacterial cellulose (BC) based antibacterial dry film, its component comprises: Bacterial cellulose dry film and peroxide antibacterial, the drug loading of bacterial cellulose (BC) based antibacterial dry film are 1.14 * 10
-5～5.68 * 10
-2Mg/cm
2
Described bacterial cellulose (BC) based antibacterial dry film, its component comprises: Bacterial cellulose dry film and contain the iodine antibacterial, the drug loading of bacterial cellulose (BC) based antibacterial dry film is 0.0852～3.4071mg/cm
2
Or described bacterial cellulose (BC) based antibacterial dry film, its component comprises: Bacterial cellulose dry film and nano antibacterial agent, the drug loading of bacterial cellulose (BC) based antibacterial dry film are 0.0341～0.0568mg/cm
2
4. according to claim 2 or 3 described a kind of bacterial cellulose (BC) based antibacterial dry films that are used for acute injury, it is characterized in that: described chloride antibacterial is selected from one or more the mixture in sodium hypochlorite, calcium hypochlorite or the Efficacious Disinfeitant; The peroxide antibacterial is selected from one or more the mixture in hydrogen peroxide, peracetic acid, the chlorine dioxide; Contain the iodine antibacterial and be selected from one or more mixture in iodine tincture, the povidone iodine; Nano antibacterial agent is selected from nano-TiO
2Granule, nano zine oxide, be loaded with Ag
+Nano material, be loaded with TiO
2Nano material in one or more mixture.
5. a kind of bacterial cellulose (BC) based antibacterial dry film according to claim 1 is characterized in that: described bacterial cellulose (BC) based antibacterial dry film can absorb the moisture content of 25～30 times of self dry weights.
6. preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury comprises:
(1) processing of BC film
Bacterial cellulose BC wet film is cut into diaphragm, through pretreatment or standby after need not to handle;
(2) preparation of antimicrobial
Inorganic antiseptic is made into the antimicrobial that concentration is 0.01～5wt%;
(3) preparation of the antibiotic dry film of BC
The BC diaphragm of step (1) preparation be impregnated in above-mentioned antimicrobial 24～30h, and drying makes the antibiotic dry film of BC.
7. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 6 is characterized in that: the inorganic antiseptic in the described step (2) is chloride antibacterial, peroxide antibacterial, contain iodine antibacterial or nano antibacterial agent; Wherein, chloride antibacterial is dissolved in the deionized water, is made into the antimicrobial that concentration is 0.1～3mg/L; The peroxide antibacterial is dissolved in the deionized water, is made into the antimicrobial that concentration is 0.1～500mg/L; To contain the iodine antibacterial and be dissolved in 75% ethanol, be made into concentration and be 0.1%～4% antimicrobial; Or above-mentioned nano antibacterial agent is dissolved in the deionized water, be made into the antimicrobial that concentration is 0.3wt ‰～0.5wt ‰.
8. according to claim 6 or 7 kind of any described a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury, it is characterized in that: described chloride antibacterial is selected from one or more the mixture in sodium hypochlorite, calcium hypochlorite or the Efficacious Disinfeitant; The peroxide antibacterial is selected from one or more the mixture in hydrogen peroxide, peracetic acid, the chlorine dioxide; Contain the iodine antibacterial and be selected from one or more mixture in iodine tincture, the povidone iodine; Nano antibacterial agent is selected from nano-TiO
2Granule, nano zine oxide, be loaded with Ag
+Nano material, be loaded with TiO
2Nano material in one or more mixture.
9. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 6, it is characterized in that: the alternative method of described step (3) is for directly coating antimicrobial the BC membrane surface of step (1) preparation, and drying makes the antibiotic dry film of BC again.
10. according to claim 6 or 9 described a kind of preparation methoies that are used for the bacterial cellulose (BC) based antibacterial dry film of acute injury, it is characterized in that: the pretreatment in the described step (1) comprises adopts rotary evaporation, squeezing or exsiccant method to remove the moisture of BC diaphragm 50%-100%.
11. according to claim 6 or 9 described a kind of preparation methoies that are used for the bacterial cellulose (BC) based antibacterial dry film of acute injury, it is characterized in that: the drying in the described step (3) is normal temperature drying, vacuum drying or lyophilization.
12. application that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury at preparation acute injury medical material.
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| CN2011100096763A Pending CN102058897A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose based antibacterial dry film applied to acute injury as well as preparation method and application thereof |
| CN2011100096373A Pending CN102091346A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application thereof |
| CN201110009673XA Pending CN102178974A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6241812B1 (en) * | 1998-02-06 | 2001-06-05 | Pharmacia Corporation | Acid-stable and cationic-compatible cellulose compositions and methods of preparation |
| WO2005003366A1 (en) * | 2003-07-03 | 2005-01-13 | Politechnika Lodzka | A method for the production of bacterial cellulose |
| CN101264335A (en) * | 2008-05-07 | 2008-09-17 | 东华大学 | Bacteria cellulose membrane containing silver chloride nano particle and preparation and application thereof |
| CN101745141A (en) * | 2010-01-15 | 2010-06-23 | 东华大学 | Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182561A (en) * | 2007-12-05 | 2008-05-21 | 天津大学 | Nanometer bacteria cellulose aldehyde grouping modified method |
| CN101509026A (en) * | 2009-03-27 | 2009-08-19 | 上海应用技术学院 | Bacteria cellulose compound film, preparation and uses thereof |
| CN101586309B (en) * | 2009-06-18 | 2012-05-09 | 北京科技大学 | Preparation for in-situ compounding simple-substance nano silvery bacteria cellulose membrane |
-
2010
- 2010-01-15 CN CN201010022842A patent/CN101745141A/en active Pending
-
2011
- 2011-01-17 CN CN2011100096763A patent/CN102058897A/en active Pending
- 2011-01-17 CN CN2011100096373A patent/CN102091346A/en active Pending
- 2011-01-17 CN CN201110009673XA patent/CN102178974A/en active Pending
- 2011-01-17 CN CN2011100096142A patent/CN102178973A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6241812B1 (en) * | 1998-02-06 | 2001-06-05 | Pharmacia Corporation | Acid-stable and cationic-compatible cellulose compositions and methods of preparation |
| WO2005003366A1 (en) * | 2003-07-03 | 2005-01-13 | Politechnika Lodzka | A method for the production of bacterial cellulose |
| CN101264335A (en) * | 2008-05-07 | 2008-09-17 | 东华大学 | Bacteria cellulose membrane containing silver chloride nano particle and preparation and application thereof |
| CN101745141A (en) * | 2010-01-15 | 2010-06-23 | 东华大学 | Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof |
| CN102058897A (en) * | 2010-01-15 | 2011-05-18 | 东华大学 | Bacterial cellulose based antibacterial dry film applied to acute injury as well as preparation method and application thereof |
| CN102178973A (en) * | 2010-01-15 | 2011-09-14 | 东华大学 | Bacterial cellulose based antibacterial dry film for treating acute injury and preparation method and application thereof |
| CN102178974A (en) * | 2010-01-15 | 2011-09-14 | 东华大学 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application |
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|---|---|---|---|---|
| CN104874015A (en) * | 2015-06-05 | 2015-09-02 | 四川大学 | Bacterial-cellulose dressing with antibacterial and bacteriostatic functions and preparation method as well as application thereof |
| CN104874015B (en) * | 2015-06-05 | 2017-06-16 | 四川大学 | A kind of bacteria cellulose dressing with antibacterial bacteriostatic function and preparation method and application |
| CN109200326A (en) * | 2018-07-19 | 2019-01-15 | 丁力行 | Dressing and bandage for wound healing |
| CN112210126A (en) * | 2019-07-12 | 2021-01-12 | 郭敏 | Preparation method of antibacterial bacterial cellulose |
| CN112972750A (en) * | 2021-03-23 | 2021-06-18 | 维尼健康（深圳）股份有限公司 | Antibacterial and disinfectant nanofiber medical dressing and preparation method thereof |
| CN112972750B (en) * | 2021-03-23 | 2021-11-19 | 维尼健康（深圳）股份有限公司 | Antibacterial and disinfectant nanofiber medical dressing and preparation method thereof |
| CN113384736A (en) * | 2021-06-25 | 2021-09-14 | 东华大学 | Pullulan-zinc oxide hybrid nanofiber/bacterial cellulose composite functional dressing and preparation method thereof |
| TWI789072B (en) * | 2021-10-25 | 2023-01-01 | 百芮國際股份有限公司 | Biocellulose dry dressing and manufacturing method thereof |
| CN115487340A (en) * | 2022-10-31 | 2022-12-20 | 振德医疗用品股份有限公司 | Bacterial cellulose dressing loaded with active oxygen and preparation method thereof |
| CN115487340B (en) * | 2022-10-31 | 2023-11-17 | 振德医疗用品股份有限公司 | Bacterial cellulose dressing loaded with active oxygen and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN102178973A (en) | 2011-09-14 |
| CN101745141A (en) | 2010-06-23 |
| CN102178974A (en) | 2011-09-14 |
| CN102058897A (en) | 2011-05-18 |
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