CN101745141A - Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof - Google Patents
Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a bacterial cellulose (BC) based antibacterial dry film applied to acute injury, comprising the following components: a BC dry film and an antibacterial agent, wherein the drug loading rate of the BC based antibacterial dry film is 0.0114-0.2027mg/cm2. A preparation method of the BC based antibacterial dry film comprises the steps of: after implanting production strains of the BC into a seed culture medium for propagation culture, transplanting the strain to a fermentation medium for static culture, obtaining a BC wet film for soaking and water-bath, cutting the wet film into sheets for drying, dipping the sheets in a prepared antibacterial agent solution for the secondary drying to obtain the BC based antibacterial dry film. In the invention, the BC based antibacterial dry film has the advantages of light and soft texture, good biocompatibility, high hygroscopicity, good air permeability, durable antibiotic property and the like; in addition, the preparation method is simple, convenient and easy in operation and has low cost; in the practical application process, the BC based antibacterial dry film can be sheared into any shapes, is not adhered, does not irritate to skin, can be rapidly degraded in the environment after being abandoned, and can be taken as a green and environment-friendly functional antibacterial dressing.
Description
Technical field
The invention belongs to Bacterial cellulose base antibacterial film and its production and application field, particularly a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury and its production and application.
Background technology
Bacterial cellulose (Bacterial Cellulose, BC) be a kind of by the synthetic native cellulose of microorganism, from chemical molecular formula, BC and common plant cellulose do not have any difference, but give its high mechanical strength and biocompatibility because of physics, the chemical constitution of uniqueness.The BC that is shaped is made of the fiber of highly crystalline body and highly single guiding banded superfine fibre, intricately is intertwined and forms unique tridimensional network, the unique tridimensional network of BC inside has a lot " duct ", high ventilation, water permeability are arranged, can absorb 60～700 times of moisture content to its dry weight, these moisture are that the form with Free water exists.Use BC pastes material as wound can absorb wound blood and tissue fluid rapidly, prevents that wound infection from suppurating, and can provide moistening environment to promote wound healing and ease the pain again near the tissue regeneration the chronic wounds.Simultaneously cellulose not can with the wound adhesion, can not cause the secondary injury, do not have residual when peeling off yet.
At present report mainly is based on wet film with Bacterial cellulose film preparation wound dressing, and mostly is applied to chronic trauma, such as decubital ulcer, scald, cold injury etc.A kind of trade name is gone on the market the BC wound dressing of Biofill.It is actually with the regulation and control of the fiber content in the wet BC film extremely to a certain degree, makes the BC film can absorb wound tissue's liquid and prevents suppurative infection, can promote the different bacteria cellulose material of a kind of moisture content of wound healing for chronic wounds provides moistening environment again.Studies show that this BC dressing can more effectively promote wound healing in the application facet of chronic trauma than the dressing of other materials.Another BC wound dressing that is called Xcell also is used to promote the healing of chronic trauma, equally also shows good therapeutic effect.But above these BC dressing itself do not have anti-microbial property prevents wound infection.Xylos company releases the improvement BC dressing of a kind of antibiotic Xcell by name on the basis of original product Xcell, and goes on the market in the U.S. in 2003.Simultaneously also there are some researchs to give BC anti-microbial property by antibacterial such as composite nano silver or chitosan on BC.
But more frequent acute injury aspect takes place in daily life, and the application of BC dressing and research are then seldom.And be used for aspect the acute injury, the application of BC wet film is subjected to bigger restriction, than dry film in various degree decline is arranged all as fluid absorbents such as permeability, blood and tissue fluids.Be compared to wet film simultaneously, carrying of dry film is more convenient, and the comfort level of acute wounds also is better than wet film.At present, do not see the research and development report of the bacterial cellulose (BC) based antibacterial dry film that is used for acute injury both at home and abroad as yet.
Summary of the invention
Technical problem to be solved by this invention provides a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury and its production and application, advantages such as the antibiotic dry film of this BC has the light softness of quality, good biocompatibility, hygroscopicity height, good permeability, and antibiotic property is lasting; And preparation method is simple and easy to do, and is with low cost, can be used as a kind of functional antibiosis dressing of environmental protection, has a good application prospect.
A kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury of the present invention, its component comprises: Bacterial cellulose dry film and cationic surfactant class antibacterial, the drug loading of bacterial cellulose (BC) based antibacterial dry film is 0.0114～0.2027mg/cm
2
Described cationic surfactant class antibacterial is selected from one or more the mixture in benzalkonium chloride (dodecyl dimethyl benzyl ammonium chloride), benzalkonium bromide (dodecyl dimethyl benzyl ammonium bromide), domiphen bromide (have another name called domiphen, chemical name is a dodecyl dimethyl benzene oxygen ethyl ammonium bromide), the myristylpicolinum bromide (myristyl lutidines ammonium bromide) etc.;
Described bacterial cellulose (BC) based antibacterial dry film can absorb the moisture content of 25～30 times of self dry weights;
Described bacterial cellulose (BC) based antibacterial dry film can continue to discharge 24～96 hours in the time of 37 ℃;
Described bacterial cellulose (BC) based antibacterial dry film can reach the bacteriostasis rate of 97.7-99.8% to gram positive bacteria (as bacillus subtilis, staphylococcus aureus etc.).
A kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury of the present invention comprises:
(1) preparation of BC film and processing
BC is produced the inoculum concentration access liquid seed culture medium of bacterial strain by 2～3 inoculating loops, seed culture is after 24 hours under 30 ℃ of conditions, be transferred in the fermentation medium by 3～15% inoculum concentration again, 20-30 ℃ leaves standstill cultivation 1-10 days, results Bacterial cellulose BC wet film, be soaked in then in the NaOH solution of 0.5～2wt%, 70-100 ℃ of water bath processing 30-120min, remove culture medium and thalline remaining in the BC wet film, it is translucent to make the BC wet film become white, the BC wet film is cut into the disk or the difform diaphragm of different area of different-diameter, dry back is standby again;
(2) preparation of antimicrobial
Cationic surfactant class antibacterial is dissolved in the deionized water, is made into the antimicrobial that concentration is 0.01wt%～0.1785wt%;
(3) preparation of the antibiotic dry film of BC
The BC dry film of step (1) preparation be impregnated in above-mentioned antimicrobial 24～30h, and drying makes the antibiotic dry film of BC again.
It is acetic acid Pseudomonas (Acetobacter sp.) that BC in the described step (1) produces bacterial strain, Bacterium gluconicum belongs to (Gluconobacter sp.), gluconic acid Acetobacter sp. (Gluconacetobacter sp.), rhizobium (Rhizobium sp.), Sarcina (Sarcina sp.), Rhodopseudomonas (Pseudomounas sp.), achromobacter (Achromobactersp.), Alcaligenes (Alcaligenes sp.), Aerobacter (Aerobacter sp.), azotobacter (Azotobacter sp.), Agrobacterium (Agrobacterium sp.), Pseudomonas cepacia (Seudomonas cepacia) or campylobacter jejuni (Campylobacter jejuni);
The seed culture medium in the described step (1) and the component of fermentation medium are: mannitol, glucose, sucrose or fructose 25g, peptone or tryptone 3g, yeast extract 5g, water 1L, pH3.5-7.5,121 ℃ of sterilization 20min; Perhaps be: mannitol, glucose, sucrose or fructose 20g, yeast extract 5g, peptone or tryptone 5g, citric acid 1.15g, Na
2HPO
42.7g, water 1L, pH3.5-7.5,121 ℃ of sterilization 20min;
Antimicrobial in the described step (2) is that mass percent concentration is 0.01%～0.1785%;
Preferred concentration is the aqueous solution of the benzalkonium chloride of 0.102%～0.1785wt%;
The alternative method of described step (3) is for directly to coat BC dry film surface with antimicrobial, and drying makes the antibiotic dry film of BC again;
Drying in described step (1) and (3) is normal temperature drying, vacuum drying or lyophilization.
A kind of application that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury at preparation acute injury medical material of the present invention.
Beneficial effect
Advantages such as (1) the antibiotic dry film of BC of the present invention has the light softness of quality, good biocompatibility, hygroscopicity height, good permeability, and antibiotic property is lasting; And preparation method is simple and easy to do, and is with low cost, but suitability for industrialized production;
(2) in actual application, can be cut into different shape arbitrarily according to the situation and the characteristics of wound, adhesion is chafe not also, safety and environmental protection, abandon the back and in environment, can degrade rapidly, can be used as a kind of functional antibiosis dressing of well behaved and environmental protection.
Description of drawings
Fig. 1 is that the BC antibacterial film of Benza preparation of variable concentrations is to the inhibitory action figure of three kinds of common bacterias;
Fig. 2 is the medicament slow release figure of BC antibacterial film of the Benza preparation of concentration 0.102%;
Fig. 3 is that the BC antibacterial film of Benza preparation of concentration 0.102% is to aureus with inhibition figure;
Fig. 4 is that the BC antibacterial film of Benza preparation of concentration 0.102% is to the inhibitory action figure of bacillus subtilis;
Fig. 5 is that the BC antibacterial film of Benza preparation of concentration 0.102% is to colibacillary inhibitory action figure.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) preparation of BC film and processing
Acetobacter xylinum (Acetobacter xylinum) by the inoculum concentration of 2～3 inoculating loops in liquid seed culture medium 30 ℃ spread cultivation 24 hours after, be transferred in the fermentation medium 30 ℃ by 6% inoculum concentration again and leave standstill and cultivated results Bacterial cellulose (BC) wet film 7 days; The BC film for preparing is immersed in 1% the NaOH solution 80 ℃ of water-baths translucent until the BC film color that bleaches, and then the BC film that makes is cut into the disk that diameter is 15mm, after the freeze drier lyophilizing, and the weighing dry weight, standby;
Wherein, the component of seed culture medium and fermentation medium is: mannitol, glucose, sucrose or fructose 25g, peptone or tryptone 3g, yeast extract 5g, water 1L, pH5.0,121 ℃ of sterilization 20min;
(2) preparation of antimicrobial
The preparation of Benza: draw 85% Benza 5.9～105 μ L respectively, standardize solution obtains the Benza that concentration is 0.01wt%～0.1785wt% to 50mL;
(3) the drug loading analysis of BC antibacterial film
Is that balance blotted the surface then and swims, the weighing weight in wet base after 24 hours in 0.102% the Benza with freeze dried BC film immersion in 25mL concentration.
The BC film calculates according to following formula the drug loading of benzalkonium chloride: drug loading=(W
w-W
d) * C, wherein, W
wBe the weight in wet base after the BC film absorbs benzalkonium chloride, W
dBe the dry weight of BC film, C is the concentration of antibacterial, and experimental result sees Table 1.
The drug loading of BC film after the lyophilizing of table 1
Experimental result shows that diameter is that the BC film of 15mm can carry about 0.2046mg benzalkonium chloride through after the lyophilizing.And the area of BC membrane material is 1.7663cm
2, therefore after the secondary lyophilizing, the content of benzalkonium chloride is 0.1158mg/cm in the BC film
2
(1) preparation of BC film and processing
Gluconate pyracetobacillus (Gluconacetobacter xylinum) by the inoculum concentration of 2～3 inoculating loops in liquid seed culture medium 30 ℃ spread cultivation 24 hours after, being transferred in the fermentation medium 25 ℃ by 10% inoculum concentration again leaves standstill and cultivated results Bacterial cellulose (BC) wet film 10 days; The BC film for preparing is immersed in 1.5% the NaOH solution 90 ℃ of water-baths translucent until the BC film color that bleaches, and then the BC film that makes is cut into the disk that diameter is 15mm, and is standby after the freeze drier lyophilizing;
Wherein, the component of seed culture medium and fermentation medium is: mannitol, glucose, sucrose or fructose 20g, yeast extract 5g, peptone or tryptone 5g, citric acid 1.15g, Na
2HPO
42.7g, water 1L, pH7.0,121 ℃ of sterilization 20min;
(2) preparation of antimicrobial
The preparation of Benza: draw 85% Benza 5.9～105 μ L respectively, standardize solution obtains the Benza that concentration is 0.01wt%～0.1785wt% to 50mL;
(3) preparation of the antibiotic dry film of BC
Is in 0.01%～0.1785% the Benza behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization obtains the antibiotic dry film of BC once more.
Dull and stereotyped diffusion method is measured the bacteriostasis of anti-biotic material
Test strain: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1L, pH7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH7;
If solid medium then adds the agar of 20g.
Experimental technique
Preparation fluid medium and solid plate culture medium are inoculated into escherichia coli, staphylococcus aureus, bacillus subtilis the liquid seed culture medium from the inclined-plane seed then, and 37 ℃ of constant temperature culture got seed liquor in 12 hours; Draw the 0.1mL seed liquor to the solid plate culture medium, coating evenly; The BC antibacterial film for preparing is tiled in dull and stereotyped central authorities, and 37 ℃ of constant temperature are inverted and were cultivated 24 hours, and the footpath of measuring inhibition zone is poor, the results are shown in Figure 1.
Experiment shows: the antibiotic dry film of BC is fungistatic effect preferably all to be arranged at 0.102%～0.1785% o'clock in benzalkonium chloride concentration, and is 0.102% o'clock in the concentration of benzalkonium chloride, to the stimulation minimum of skin.
Embodiment 3
(1) preparation of BC film and processing
Oxidizing glucose acidfast bacilli (Gluconobacter oxydans) by the inoculum concentration of 2～3 inoculating loops after 30 ℃ of liquid seed culture mediums spread cultivation 24 hours, being transferred in the fermentation medium 20 ℃ by 15% inoculum concentration again leaves standstill and cultivated results Bacterial cellulose (BC) wet film 10 days; The BC film for preparing is immersed in 1% the NaOH solution 80 ℃ of water-baths translucent until the BC film color that bleaches, and then the BC film that makes is cut into the disk that diameter is 15mm, and is standby after the freeze drier lyophilizing;
Wherein, the component of seed culture medium and fermentation medium is: mannitol, glucose, sucrose or fructose 25g, peptone or tryptone 3g, yeast extract 5g, water 1L, pH4.0,121 ℃ of sterilization 20min;
(2) preparation of antimicrobial
The preparation of domiphen bromide solution: draw 85% domiphen bromide solution 5.9～105 μ L respectively, standardize solution is to 50mL, and obtaining concentration is the domiphen bromide solution of 0.01wt%～0.1785wt%;
(3) preparation of the antibiotic dry film of BC
Is in 0.01%～0.1785% the domiphen bromide solution behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization obtains antibiotic dry film once more.
The water absorbing properties test of the anti-film of BC
After the freeze dried BC anti-biotic material of secondary weighed, be immersed in 24h in the deionized water, blot the surface then and swim, weigh.The expansion rate of BC antibacterial film is calculated according to following formula: expansion rate=(W
s-W
d)/W
d, W wherein
sBe the weight in wet base after the BC film expands, W
dBe the original dry weight of BC film, experimental result sees Table 2.
The expansion rate of BC film after the lyophilizing of table 2 secondary
Experimental result shows: diameter is the BC film of 15mm is about own dry weight through its absorbability after the secondary lyophilizing 26.20 times.
(1) preparation of BC film and processing
Acetobacter xylinum (Acetobacter xylinum) by the inoculum concentration of 2～3 inoculating loops in liquid seed culture medium 30 ℃ spread cultivation 24 hours after, be transferred in the fermentation medium 30 ℃ by 6% inoculum concentration again and leave standstill and cultivated results Bacterial cellulose (BC) wet film 7 days; The BC film for preparing is immersed in 1% the NaOH solution 80 ℃ of water-baths translucent until the BC film color that bleaches, and then the BC film that makes is cut into the disk that diameter is 15mm, and is standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
The preparation of Benza: draw 85% Benza 5.9～105 μ L respectively, standardize solution obtains the Benza that concentration is 0.01wt%～0.1785wt% to 50mL;
(3) preparation of the antibiotic dry film of BC
Is in 0.01%～0.1785% the Benza after 24 hours with freeze dried BC film immersion in 25mL concentration, and lyophilization obtains antibiotic dry film once more.
The medicament slow release of BC antibacterial film is measured
By benzalkonium chloride is carried out uv scan, choose 263nm as measuring wavelength;
Medicament slow release: the BC antibacterial film for preparing is immersed in the beaker that the 25mL deionized water is housed, and 37 ℃ of waters bath with thermostatic control are surveyed the 263nm light absorption value every sampling in 2 hours, the results are shown in Figure 2.
Experimental result shows: the antibiotic dry film of BC by secondary lyophilizing immersion process for preparing can effectively discharge benzalkonium chloride more than 24 hours, and when 24h, the slow release rate of benzalkonium chloride is about 66%.
Embodiment 5
(1) preparation of BC film and processing
Acetobacter xylinum (Acetobacter xylinum) by the inoculum concentration of 2～3 inoculating loops in liquid seed culture medium 30 ℃ spread cultivation 24 hours after, being transferred in the fermentation medium 30 ℃ by 10% inoculum concentration again leaves standstill and cultivated results Bacterial cellulose (BC) wet film 7 days; The BC film for preparing is immersed in 1% the NaOH solution 100 ℃ of water-baths translucent until the BC film color that bleaches, and then the BC film that makes is cut into the disk that diameter is 15mm, and is standby after the freeze drier lyophilizing;
(2) preparation of antimicrobial
The preparation of Benza: draw 85% Benza 5.9～105 μ L respectively, standardize solution obtains the Benza that concentration is 0.01wt%～0.1785wt% to 50mL;
(3) preparation of the antibiotic dry film of BC
Is in 0.01%～0.1785% the Benza behind the 24h with freeze dried BC film immersion in 25mL concentration, and lyophilization obtains antibiotic dry film once more.
The BC antibacterial film detects test strain to the Growth Inhibition of escherichia coli, staphylococcus aureus, bacillus subtilis: escherichia coli, staphylococcus aureus, bacillus subtilis;
Culture medium:
(1) escherichia coli: tryptone 10g, yeast extract 5g, NaCl 10g, water 1L, pH7;
(2) staphylococcus aureus: Carnis Bovis seu Bubali cream 3g, peptone 5g, NaCl 50g, water 1L, pH7.4;
(3) bacillus subtilis: glucose 20g, peptone 15g, Carnis Bovis seu Bubali cream 0.5g, NaCl 5g, water 1L, pH7.
Experimental technique
The preparation fluid medium: escherichia coli, staphylococcus aureus, bacillus subtilis inclined-plane seed are inoculated in the liquid seed culture medium, and 37 ℃ of constant temperature culture 12h get seed liquor.In 100mL liquid culture collection base, add blank BC film, 0.102% benzalkonium chloride BC antibacterial film respectively.Draw the 5mL seed liquor then respectively and insert wherein, behind the mixing, 37 ℃ of constant temperature culture 24h, timing sampling is measured the optical density value OD of culture fluid
600, to make growth curve then and calculate bacteriostasis rate, experimental result is seen Fig. 3-5.
Experimental result shows: the BC antibacterial film by secondary lyophilizing immersion process for preparing can the strong inhibition staphylococcus aureus and bacillus subtilis more than 24 hours, has reached 99.8% and 97.7% bacteriostasis rate respectively; And it is not remarkable to colibacillary inhibition effect.
Claims (10)
1. bacterial cellulose (BC) based antibacterial dry film that is used for acute injury, its component comprises: Bacterial cellulose dry film and cation surface activating antibacterial, the drug loading of bacterial cellulose (BC) based antibacterial dry film are 0.0114～0.2027mg/cm
2
2. a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury according to claim 1 is characterized in that: described cation surface activating antibacterial is selected from one or more the mixture in benzalkonium chloride, benzalkonium bromide, domiphen bromide, the myristylpicolinum bromide.
3. a kind of bacterial cellulose (BC) based antibacterial dry film that is used for acute injury according to claim 1 is characterized in that: described bacterial cellulose (BC) based antibacterial dry film can absorb the moisture content of 25～30 times of self dry weights, can continue to discharge 24～96 hours in the time of 37 ℃; Can reach 97.7～99.8% bacteriostasis rate to gram positive bacteria.
4. preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury comprises:
(1) preparation of BC film and processing
BC is produced the inoculum concentration access liquid seed culture medium of bacterial strain by 2～3 inoculating loops, seed culture is after 24 hours under 30 ℃ of conditions, be transferred in the fermentation medium by 3～15% inoculum concentration again, 20-30 ℃ leaves standstill cultivation 1-10 days, and results Bacterial cellulose BC wet film is soaked in the NaOH solution of 0.5～2wt% then, 70-100 ℃ of water bath processing 30-120min, it is translucent to make the BC wet film become white, is cut into diaphragm again, and dry back is standby;
(2) preparation of antimicrobial
Above-mentioned cation surface activating antibacterial is dissolved in the deionized water, is made into the antimicrobial that concentration is 0.01wt%～0.1785wt%;
(3) preparation of the antibiotic dry film of BC
The BC dry film of step (1) preparation be impregnated in above-mentioned antimicrobial 24～30h, and drying makes the antibiotic dry film of BC again.
5. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 4 is characterized in that: it is acetic acid Pseudomonas, Bacterium gluconicum genus, gluconic acid Acetobacter sp., rhizobium, Sarcina, Rhodopseudomonas, achromobacter, Alcaligenes, Aerobacter, azotobacter, Agrobacterium, Pseudomonas cepacia or campylobacter jejuni that the BC in the described step (1) produces bacterial strain.
6. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 4, it is characterized in that: the seed culture medium in the described step (1) and the component of fermentation medium are: mannitol, glucose, sucrose or fructose 25g, peptone or tryptone 3g, yeast extract 5g, water 1L, pH3.5-7.5,121 ℃ of sterilization 20min; Or mannitol, glucose, sucrose or fructose 20g, yeast extract 5g, peptone or tryptone 5g, citric acid 1.15g, Na
2HPO
42.7g, water 1L, pH3.5-7.5,121 ℃ of sterilization 20min.
7. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 4 is characterized in that: the antimicrobial in the described step (2) is that mass percent concentration is 0.01%～0.1785%.
8. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 4, it is characterized in that: the alternative method of described step (3) is for directly to coat BC dry film surface with antimicrobial, and drying makes the antibiotic dry film of BC again.
9. a kind of preparation method that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury according to claim 4 is characterized in that: the drying in described step (1) and (3) is normal temperature drying, vacuum drying or lyophilization.
10. application that is used for the bacterial cellulose (BC) based antibacterial dry film of acute injury at preparation acute injury medical material.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010022842A CN101745141A (en) | 2010-01-15 | 2010-01-15 | Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof |
| CN2011100096763A CN102058897A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose based antibacterial dry film applied to acute injury as well as preparation method and application thereof |
| CN2011100096142A CN102178973A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose based antibacterial dry film for treating acute injury and preparation method and application thereof |
| CN2011100096373A CN102091346A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application thereof |
| CN201110009673XA CN102178974A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application |
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| CN2011100096142A Pending CN102178973A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose based antibacterial dry film for treating acute injury and preparation method and application thereof |
| CN2011100096763A Pending CN102058897A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose based antibacterial dry film applied to acute injury as well as preparation method and application thereof |
| CN201110009673XA Pending CN102178974A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application |
| CN2011100096373A Pending CN102091346A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application thereof |
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| CN2011100096763A Pending CN102058897A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose based antibacterial dry film applied to acute injury as well as preparation method and application thereof |
| CN201110009673XA Pending CN102178974A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application |
| CN2011100096373A Pending CN102091346A (en) | 2010-01-15 | 2011-01-17 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091346A (en) * | 2010-01-15 | 2011-06-15 | 东华大学 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application thereof |
| CN102552965A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Method for preparing nano-cellulose antibacterial composite material through on-line culture |
| CN102727926A (en) * | 2011-12-12 | 2012-10-17 | 北京科技大学 | Preparation method of polysaccharide-nanometer bacterial cellulose composite wound dressing |
| CN102764447A (en) * | 2012-07-27 | 2012-11-07 | 武汉人福医疗用品有限公司 | Hydrogel dressing and preparation process thereof |
| CN103239731A (en) * | 2013-04-26 | 2013-08-14 | 海南光宇生物科技有限公司 | Edible buccal patch for treating dental ulcer |
| CN105497967A (en) * | 2016-01-20 | 2016-04-20 | 李绍旭 | Dry bacterial cellulose membrane dressing |
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- 2011-01-17 CN CN2011100096763A patent/CN102058897A/en active Pending
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Also Published As
| Publication number | Publication date |
|---|---|
| CN102058897A (en) | 2011-05-18 |
| CN102091346A (en) | 2011-06-15 |
| CN102178974A (en) | 2011-09-14 |
| CN102178973A (en) | 2011-09-14 |
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