CN102177863B - Method for inducing hormone soaked loaches - Google Patents
Method for inducing hormone soaked loaches Download PDFInfo
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- CN102177863B CN102177863B CN201110138802A CN201110138802A CN102177863B CN 102177863 B CN102177863 B CN 102177863B CN 201110138802 A CN201110138802 A CN 201110138802A CN 201110138802 A CN201110138802 A CN 201110138802A CN 102177863 B CN102177863 B CN 102177863B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a method for inducing hormone soaked loaches. The method comprises the following steps: (1) selecting a mature loach parent in water at the temperature of between 21 and 29 DEG C; and (2) taking a mixture of domperidone maleate, luteotropin releasing hormone d-ala analog and chorionic gonadotropin as oxytocic hormones, and soaking the loach parent with a soaking solution which is prepared by mixing the oxytocic hormone, pentoxifylline and edible oil in normal saline, wherein the dosage of the domperidone maleate is between 100 and 300mg/kg, the dosage of the luteotropin releasing hormone d-ala analog is between 200 and 600mu g/kg, the dosage of the chorionic gonadotropin is between 15,000 and 30,000IU/kg, and the dosage of the normal saline is between 0.2 and 0.3kg/kg; and when the loach parent is soaked, the temperature of the soaking solution is controlled to be between 21 and 29 DEG C, and the soaking time is controlled to be between 0.5 and 8 hours. The aim of inducing the loach parent can be realized through soaking instead of injection.
Description
Technical field
The present invention relates to the method for hastening parturition of a kind of loach, relate in particular to the method for hastening parturition that a kind of hormone soaks loach.
Background technology
Loach (Misgurnus arguillicaudatus) is under the jurisdiction of Cypriniformes, Cobitidae, and loach belongs to, and is commonly called as " powder loach ".It distributes wide, and adaptability is strong, is a kind of omnivorous small-sized economic freshwater fish.The loach delicious meat has higher nutritive value and medical value, receives liking of domestic and international consumer deeply.It is big that loach is cultured development potentiality, but loach natural seeding, wild fry quantity can not effective supply breeds needs, and this has restricted the development of loach aquaculture industry.
The method of conventional injection oxytocic hormone can run into the problem that loach is difficult to arrest.In the artificial propagation process, because the loach individuality is little, arresting injection one by one needs a lot of times, influence damage parent.
Chinese patent CN200710114040.9 discloses sustained-release incretion particle of a kind of inducing maturation of Oplegnathus fasciatus gonad and preparation method thereof, the troublesome poeration that the direct injection hormone method of solving exists, to problems such as the stimulation of fish body are big.It will be released control thing (cholesterol, cellulose), luteinizing hormone releasing hormone and maleic acid DOM and be mixed and made into sustained-release incretion particle by certain content, and every sustained-release incretion particle contains the hybrid particles of cholesterol 22-23mg, cellulose 1.5-2.5mg, Luteinizing hormone releasing-hormone A3 1.0-1.2mg, maleic acid DOM 35-45mg.This invention is used for inducing maturation of Oplegnathus fasciatus gonad, prolongs the ovulation period of parent population, reduces the damage to parent population, has guaranteed carrying out smoothly of production.This scheme is made into feed granules with hormone, directly implants back or the nursing of fish, and there are shortcomings such as administering mode efficient is low, effect is uncertain in this kind scheme.
Chinese patent CN200310105462.1 discloses a kind of cold water fish and has used slow-release hormone; It is characterized in that containing the analog or the human chorionic gonadotropin of water, gelatin, maleic acid DOM and salmon gonadotropin releasing hormone analogues or gonadotropin-releasing hormone (GRH) or gonadotropin releasing hormone analogues or salmon gonadotropin-releasing hormone (GRH) or mammality luteinizing hormone-releasing hormone or mammality luteinizing hormone-releasing hormone, their part by weight or unit scope are 100 respectively, 4-6,0.5-1.2 and 0.01-0.03 or 50000-150000.Its advantage is to reach the purpose that hormone slowly is provided for a long time, and easy to use, time saving and energy saving, saves money.But, also be to arrive in parent's body, thereby slowly discharge the permanent release that reaches drug effect after making liquid medicine in parent's body, become solid that this damage to the parent is bigger through injecting liquid medicine.
Summary of the invention
The objective of the invention is for solving the problems of the technologies described above, provide a kind of hormone to soak the method for hastening parturition of loach.
Above-mentioned technical purpose of the present invention is achieved through following technical scheme:
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent of selective maturation places 21～29 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 100～300mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 200～600 μ g/kg, and the human chorionic gonadtropin consumption is 15000～30000IU/kg, physiological saline 0.2～0.3kg/kg; During immersion, the soak temperature is controlled at 21～29 ℃, and soak time was controlled at 0.5～8 hour.
The enforcement of technique scheme of the present invention, save time and the labour in, remove wound to the loach parent, realized hastening parturition to loach.Spawning rate can be controlled in 20%～100%.
Among the present invention, the consumption of maleic acid DOM, luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and physiological saline all is to calculate with every kilogram of loach parent's body weight; Like the luteotropin releasing hormone d-ala analog consumption is 200～600 μ g/kg, is meant that the luteotropin releasing hormone d-ala analog that every kilogram of loach parent will use is 200～600 μ g.
The consumption of ethyl ketone theobromine and edible oil is with the volume calculation of said soak; Like ethyl ketone theobromine 200 mg/L, be meant that the amount that contains the ethyl ketone theobromine is 200 mg in every liter the soak.
The ethyl ketone theobromine uses as the improving blood flow agent, when effective object is human body usually; It can reduce blood viscosity through recovering to receive the erythrocytic deformability of pathological lesion, suppresses platelet aggregation; Blood fluidity is improved, thereby increase the trophism microcirculation of impaired part.And in the present invention, the ethyl ketone theobromine uses as short skin absorbent, and it can make oxytocic hormone of the present invention that the efficient of hastening parturition of loach is increased by 5～20% effectively.
Edible oil uses as antifoaming agent among the present invention.When many loach flock together, be easy to generate foam, when soaking loach with soak according to the invention, the significant foam of generation can make spawning rate reduce.
Preferred as technique scheme, the consumption of said ethyl ketone theobromine is 180～250 mg/L.
Preferred as technique scheme, the consumption of said edible oil is 3～8 mg/L.
Preferred as technique scheme, the soak temperature is controlled at 22～25 ℃, and soak time was controlled at 2～6 hours.
Preferred as technique scheme, said loach parent's monomer weight is controlled at 20～30g.
In sum, the present invention has following beneficial effect:
Enforcement of the present invention can replace the purpose that the injection realization is hastened parturition to the loach parent through soaking.It not only saves the time of arresting and injecting to the loach parent, reduces the wound that the loach parent is caused.Enforcement of the present invention can address the above problem at one stroke; Simultaneously, spawning rate of the present invention can reach 100%.
Embodiment
This specific embodiment only is to explanation of the present invention; It is not a limitation of the present invention; Those skilled in the art can make the modification that does not have creative contribution to present embodiment as required after reading this specification, but as long as in claim scope of the present invention, all receive the protection of Patent Law.
Embodiment one
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 23kg of selective maturation places 21 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 100mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 200 μ g/kg; The human chorionic gonadtropin consumption is 15000IU/kg; Physiological saline 0.2kg/kg, the consumption of ethyl ketone theobromine are 180 mg/L, and the consumption of edible oil is 3mg/L; During immersion, the soak temperature is controlled at 21 ℃, and soak time was controlled at 8 hours.Spawning rate is 37%.
Embodiment two
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 25kg of selective maturation places 22 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 120mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 225 μ g/kg; The human chorionic gonadtropin consumption is 15500IU/kg; Physiological saline 0.2kg/kg, the consumption of ethyl ketone theobromine are 180 mg/L, and the consumption of edible oil is 3mg/L; During immersion, the soak temperature is controlled at 22 ℃, and soak time was controlled at 7 hours.Spawning rate is 54%.
Embodiment three
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 25kg of selective maturation places 23 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 145mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 250 μ g/kg; The human chorionic gonadtropin consumption is 15750IU/kg; Physiological saline 0.2kg/kg, the consumption of ethyl ketone theobromine are 200 mg/L, and the consumption of edible oil is 4mg/L; During immersion, the soak temperature is controlled at 23 ℃, and soak time was controlled at 6 hours.Spawning rate is 69%.
Embodiment four
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 30kg of selective maturation places 24 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 150mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 250 μ g/kg; The human chorionic gonadtropin consumption is 16000IU/kg; Physiological saline 0.2kg/kg, the consumption of ethyl ketone theobromine are 200 mg/L, and the consumption of edible oil is 4mg/L; During immersion, the soak temperature is controlled at 24 ℃, and soak time was controlled at 4 hours.Spawning rate is 100%.
Embodiment five
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 25kg of selective maturation places 25 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 200mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 300 μ g/kg; The human chorionic gonadtropin consumption is 17000IU/kg; Physiological saline 0.3kg/kg, the consumption of ethyl ketone theobromine are 225 mg/L, and the consumption of edible oil is 6mg/L; During immersion, the soak temperature is controlled at 25 ℃, and soak time was controlled at 5 hours.Spawning rate is 63%.
Embodiment six
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 25kg of selective maturation places 26 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 250mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 500 μ g/kg; The human chorionic gonadtropin consumption is 20000IU/kg; Physiological saline 0.3kg/kg, the consumption of ethyl ketone theobromine are 225 mg/L, and the consumption of edible oil is 6mg/L; During immersion, the soak temperature is controlled at 26 ℃, and soak time was controlled at 3 hours.Spawning rate is 46%.
Embodiment seven
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 25kg of selective maturation places 27 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 300mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 600 μ g/kg; The human chorionic gonadtropin consumption is 25000IU/kg; Physiological saline 0.3kg/kg, the consumption of ethyl ketone theobromine are 250 mg/L, and the consumption of edible oil is 8mg/L; During immersion, the soak temperature is controlled at 27 ℃, and soak time was controlled at 2 hours.Spawning rate is 29%.
Embodiment eight
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 25kg of selective maturation places 28 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 300mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 600 μ g/kg; The human chorionic gonadtropin consumption is 30000IU/kg; Physiological saline 0.3kg/kg, the consumption of ethyl ketone theobromine are 250 mg/L, and the consumption of edible oil is 8mg/L; During immersion, the soak temperature is controlled at 28 ℃, and soak time was controlled at 1 hour.Spawning rate is 21%.
Embodiment nine
Hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent 25kg of selective maturation places 29 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 300mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 600 μ g/kg; The human chorionic gonadtropin consumption is 30000IU/kg; Physiological saline 0.3kg/kg, the consumption of ethyl ketone theobromine are 250 mg/L, and the consumption of edible oil is 8mg/L; During immersion, the soak temperature is controlled at 28 ℃, and soak time was controlled at 0.5 hour.Spawning rate is 20%.
Can find that through above embodiment after the amount of oxytocic hormone acquired a certain degree, the amount of oxytocic hormone did not have very big influence to spawning rate; Influencing big factor is soak time and soaking temperature.When temperature was higher, as 29 ℃, this moment, the osmotically active of soak was maximum, and therefore required soak time can be very short, and as 0.5 hour, because this temperature is unsuitable concerning the loach parent, although therefore hormone concentration is high, its efficient of hastening parturition was not high yet.When temperature is lower, as 22 ℃, this moment, the activity of each composition in the soak was lower; Therefore not only required soak time is long, as 7 hours, but because this temperature is comparatively suitable concerning the loach parent; Although therefore hormone concentration is low, active low, its efficient of hastening parturition still is higher.But further reduce when temperature, as 20 ℃ the time, the efficient of hastening parturition under this temperature is reduced to below 20%.
Claims (2)
1. hormone soaks the method for hastening parturition of loach, may further comprise the steps:
1. the loach parent of selective maturation places 21～29 ℃ of water;
2. with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, mix with this oxytocic hormone, ethyl ketone theobromine and edible oil and in physiological saline, to process soak the loach parent is soaked; Wherein, maleic acid DOM consumption is 100～300mg/kg, and the luteotropin releasing hormone d-ala analog consumption is 200～600 μ g/kg, and the human chorionic gonadtropin consumption is 15000～30000IU/kg, physiological saline 0.2～0.3kg/Kg; During immersion, the soak temperature is controlled at 21～29 ℃, and soak time was controlled at 0.5～8 hour;
Consumption 180～the 250mg/L of said ethyl ketone theobromine;
The consumption of said edible oil is 3～8 mg/L;
Said loach parent's monomer weight is controlled at 20～30g;
The consumption of said maleic acid DOM, luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and physiological saline all is to calculate with every kilogram of loach parent's body weight;
The consumption of said ethyl ketone theobromine and edible oil is with the volume calculation of said soak.
2. hormone according to claim 1 soaks the method for hastening parturition of loach, and it is characterized in that: the soak temperature is controlled at 22～25 ℃, and soak time was controlled at 2～6 hours.
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| CN201110138802A CN102177863B (en) | 2011-05-26 | 2011-05-26 | Method for inducing hormone soaked loaches |
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| CN201110138802A CN102177863B (en) | 2011-05-26 | 2011-05-26 | Method for inducing hormone soaked loaches |
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| CN102177863B true CN102177863B (en) | 2012-10-24 |
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| CN102835336A (en) * | 2012-09-17 | 2012-12-26 | 盐城市盐都区水产技术推广站 | Method for batch breeding of loach fry |
| CN103190537B (en) * | 2013-04-09 | 2014-10-29 | 林衍峰 | Fish oytocin oral granules and preparation and using methods thereof |
| CN104160991B (en) * | 2014-06-19 | 2016-08-24 | 蚌埠市禹会天河联众水产养殖农民专业合作社 | Family's loach breeding induced spawning method |
| CN108012962A (en) * | 2017-12-26 | 2018-05-11 | 重庆医学检验试剂研究所 | A kind of pharmaceutical composition and its reagent and method for promoting aquatic livestock ovulation |
| CN109699530B (en) * | 2019-01-25 | 2021-09-07 | 佛山市嘉沃农业科技合伙企业（有限合伙） | Artificial spawning induction method for loach culture |
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| CN101647418A (en) * | 2009-09-18 | 2010-02-17 | 湖北省水产科学研究所 | Method for improving fertility rate of loach roe |
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