CN102177803A - Cuttage propagation method for clumping bamboos - Google Patents
Cuttage propagation method for clumping bamboos Download PDFInfo
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- CN102177803A CN102177803A CN 201110062629 CN201110062629A CN102177803A CN 102177803 A CN102177803 A CN 102177803A CN 201110062629 CN201110062629 CN 201110062629 CN 201110062629 A CN201110062629 A CN 201110062629A CN 102177803 A CN102177803 A CN 102177803A
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Abstract
The invention discloses a cuttage propagation method for clumping bamboos, comprising the following steps: selecting a seedling culture nursery land, and flattening the nursery land; dipping a bigslip into 100-200ppm of an IBA (iso butyl alcohol) solution or an ABT solution, or dipping in 1000-2000ppm of the IBA solution or the ABT solution; inserting the bigslip processed by a hormone into a seedling culture ditch, covering with soil and tramping; and spraying water and covering with grass. With the method, the cottage survival rate of dendrocalamus affinis, big allusion bamboo, Dendrocalamus latiflorus or Dendrocalamus brandisii is improved by above 35%, and the method has an important meaning for the industrial mass production of the bamboo seedlings and promotion afforestation.
Description
Technical field
The invention belongs to the botanical seedling culturing cultivation field, the cuttage breeding method of the bamboo that particularly grows thickly.
Background technology
Grow thickly commonly used the having of seedling growing process measure of bamboo the band root and stem of certain plants buries bar, the joint of footing the bill, bury binodal, bury three and save, bury that the root and stem of certain plants is grown seedlings and cuttage and seedling culture etc.
Cuttage and seedling culture propagating materials cost is low, and reproduction speed is fast, becomes the seedling time short, and seedling growing process is simple; Conveniently introduce a fine variety and transport; Suitable large tracts of land afforestation is grown seedlings; Female bamboo and branch are easy to get, the availability height, thereby be a kind of perfect method in the bamboo method of forestation of growing thickly.The preadaptation environment capacity is not poor but the cuttage branch is taken root, and requires management meticulous, and is difficult for taking root, and survival rate is lower, fails to form scale, industrialization production model.
Sichuan Province is after successfully introducing sinocalamus latiflorus, major part allusion quotation bamboo, the numb blue or green bamboo of support, 4 bamboo kinds of sweet imperial bamboo; the large-scale production of seedling becomes the technical bottleneck of promoting afforestation; particularly to supportting these class Hybrid Bamboo kinds such as numb blue or green bamboo; the propagating materials rareness; how to produce a large amount of seedlings rapidly, also be the technical barrier of being badly in need of breakthrough.
Summary of the invention
For addressing the above problem, the present invention aims to provide the cuttage breeding method of a kind of bamboo that grows thickly.
The cuttage breeding method of the bamboo that grows thickly provided by the invention comprises the steps:
1) Seedlings nursery ground is selected and whole ground: select that illumination condition is good, irrigation and drainage convenience, traffic convenience, loosen fertile mountain region yellow earth, purple soil or fluvial sand loam be as Seedlings nursery ground, deep-cut again to the garden smooth earlier, at least to turn over two rakes by two, choose most weeds tree root, per hectare applies the farmyard manure 37500kg～45000kg that becomes thoroughly decomposed, simultaneously soil is carried out pesticide and disinfectant and handle, reason limit, earthing is in order to draining;
2) HORMONE TREATMENT: cutting is soaked in the IBA solution or ABT solution of 100～200ppm, or is soaked in the IBA solution or ABT solution of 1000～2000ppm;
3) cuttage: hormone-treated cutting oblique cutting is gone into to grow seedlings in the ditch, and embedded depth 6cm～10cm stays and goes up joint branches and leaves most and basset, after blinding tracks tramping, and trickle lid grass.
The cuttage breeding method of the bamboo that grows thickly provided by the invention, also comprise: after described step 3), carry out conventional field management, comprise in time and water, weed a garden in good time, late June to early July then in the side of bamboo seedling by going up a slope, fertilising cave apart from bamboo seedling 20～25cm cutting depth 10～15cm, N is executed in every strain behind the rain: P: K is composite fertilizer 0.1～0.2kg of 2: 1: 1, and cover with soil, carrying out weeding in 4～May and 7～August in 1 year topdresses, topdressing amount is that every clump of 500～800g N: P: K is 2: 1: 1 a composite fertilizer, divide 2～3 spread manuer in holes into.
Wherein, the cutting described step 2) is at the IBA of 100～200ppm solution or ABT solution soaking 10h; Or in the IBA of 1000～2000ppm solution or ABT solution, soak 30s.
According to the present invention, described cutting comes from biennial major branch or annual major branch, and there are some tangible projections in preferred container made of bamboo, wicker, ratten, etc. portion of transplanting a cutting.
According to the present invention, the described bamboo that grows thickly is sinocalamus latiflorus, major part allusion quotation bamboo, the numb blue or green bamboo of support or sweet imperial bamboo.
Compared with prior art, the cuttage breeding method of this bamboo that grows thickly that provides of the present invention has crucial meaning:
The invention solves the difficulty of taking root in sinocalamus latiflorus, major part allusion quotation bamboo, the numb blue or green bamboo of support or 4 bamboo kinds of the sweet imperial bamboo cuttage and seedling culture process, the problem that survival rate is low.Using method of the present invention, the cuttage survival rate of above-mentioned 4 kinds can be brought up to more than 35%, is that the cuttage survival rate of these difficulties bamboo kind of growing thickly of taking root improves greatly, the industrialization of bamboo seedling is produced and promotes in batches afforestation have crucial meaning.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.The hormone that the present invention relates to and other medicaments unless otherwise noted, all are commercially available.
The cottage propagation of embodiment 1 sinocalamus latiflorus, major part allusion quotation bamboo
At the beginning of 4 months, support the cottage propagation of green bamboo in Yucheng District nursery, Ya'an, Sichuan Agricultural University test site.Select at first that illumination condition is good, irrigation and drainage convenience, traffic convenience, loose fertile mountain region yellow earth be as Seedlings nursery ground, deep-cut again smooth earlier, turn over two rakes by two, reach flat, thin, soft requirement, choose most weeds tree root, per hectare applies the farmyard manure 37500kg～45000kg that becomes thoroughly decomposed, and uses KMn0 simultaneously
4Carry out soil insect-killing with carbendazim solution etc. and disinfect, reason limit, earthing is in order to draining.Open the ditch of growing seedlings on the smooth good furrow face, ditch depth 15cm, furrow width 20cm.
Choose sinocalamus latiflorus or major part allusion quotation bamboo biennial (2a) major branch, biennial secondary to or annual major branch, it is qualified branch that there are some tangible projections in its branch container made of bamboo, wicker, ratten, etc. portion.With scraper the flat bamboo stalk of branch is cut down, do not hindering eye and root eye on the branch container made of bamboo, wicker, ratten, etc., and branch can not be torn, cut off branch, leave and take a bit of of branch base portion 2-3 joint, be about 30cm-40cm, sprouted the branch of branch for twig on the branch, gone up most on the joint side shoot the nodal bud, cut off other all side shoots except that leaving and taking, peel off the branch sheaths of bamboo shoots of depositing the place, expose eye.
Sinocalamus latiflorus, major part allusion quotation bamboo bamboo branch cuttage Processing Test.Experimental field be located at Pengshan County Qinglong Zhen, Yucheng District nursery, Ya'an, Sichuan agricultural university woods examination hall, and the nursery, Muchuan County is carried out.
L is all adopted in the HORMONE TREATMENT test of sinocalamus latiflorus and two bamboo kinds of major part allusion quotation bamboo
9(3
4) orthogonal design, every bamboo kind is done 2 groups of tests (long-time and high concentration short time of low concentration), and 9 combinations of every group of test of each bamboo kind repeat each sub-district 60 stem cutting bar 3 times.Orthogonal array and treatment combination see Table 1.
Cutting time is at the beginning of 4 months, carry out conventional field management after inserting, carry out conventional field management, comprise in time and water, weed a garden in good time, late June to early July then in the side of bamboo seedling by going up a slope, fertilising cave apart from bamboo seedling 25cm cutting depth 15cm, N is executed in every strain behind the rain: P: K is 2: 1: 1 composite fertilizer 0.2kg, and cover with soil, carrying out weeding in 4～May and 7～August in 1 year topdresses, topdressing amount is that every clump of 500～800g N: P: K is 2: 1: 1 a composite fertilizer, divide 2～3 spread manuer in holes into.
Year-end complete investigation survive strain number, leading thread, on the ground with totally 8 growth indexes such as under ground portion fresh weight biomass, the hat width of cloth, every Cong Zhu seedling number, height of seedling, one-level radical.
Table 1 sinocalamus latiflorus, major part allusion quotation bamboo branch cuttage low concentration experimental group L
9(3
4) the Orthogonal Experiment and Design table
(1) sinocalamus latiflorus
Each treatment combination cuttage survival rate of sinocalamus latiflorus low concentration experimental group orthogonal experiment, bamboo seedling mean height, average leading thread, every Cong Zhu seedling number, one-level lateral root, the hat width of cloth, ground and 8 characteristic index result of the tests of under ground portion fresh weight see Table 2.
Table 2 sinocalamus latiflorus low concentration group cuttage result of the test table
As shown in Table 2, each treatment combination cuttage survival rate of sinocalamus latiflorus low concentration group is between 0～63.3%, average bamboo height of seedling 0.55m～1.05m, average leading thread 0.96cm～1.56cm, several 2.0 strains of every Cong Zhu seedling～3.3 strains, 11～46 of one-level radicals, hat width of cloth 0.26m～0.78m, acrial part fresh weight 30g～357.78g, under ground portion fresh weight 50g～213.33g.
Table 3 sinocalamus latiflorus low concentration group empirical risk probability (F check P value) analytical table
His-and-hers watches 2 results carry out variance analysis and get each proterties risk probability table 3, the result shows: sinocalamus latiflorus low concentration group cuttage survival rate all has utmost point significant difference in branch type, medicine and concentration, there were significant differences to the hat width of cloth for drug concentration, and one-level radical and branch type and drug concentration reach significant difference.All the other there are no significant differences.
As shown in Table 4, each treatment combination cuttage survival rate of sinocalamus latiflorus high concentration group is between 0～70.0%, average bamboo height of seedling 0.61m～0.99m, average bamboo seedling leading thread is 1.09cm～1.48cm, several 1～4 strains of every Cong Zhu seedling, 23～48 of one-level radicals, hat width of cloth 0.47m～0.82m, acrial part fresh weight 105.00g～380.42g, under ground portion fresh weight 85.00g～241.75g.
Table 4 sinocalamus latiflorus high concentration group cuttage result of the test
His-and-hers watches 4 data are carried out variance analysis and are drawn table 5, and the result shows: high concentration experimental group branch type and medicine reach utmost point significant difference level aspect survival rate.
Table 5 sinocalamus latiflorus high concentration group empirical risk probability (F check P value) analytical table
Every Cong Zhu seedling number has utmost point significant difference on the branch type, aspect medicine and concentration, reach significant difference, all the other equal indifferences.
The survival rate of low concentration group and high concentration group is carried out LSD check (table 6) show between three each levels of factor of sinocalamus latiflorus low concentration group survival rate all there were significant differences all do not have significant difference between each level of high concentration group three factors.Therefore, the best of breed of two groups of test cuttage survival rates is A1B1C3, and promptly 2a branch IBA200ppm concentration is soaked 10h.
Table 6 sinocalamus latiflorus cuttage survival rate LSD check table
For the sinocalamus latiflorus cuttage and seedling culture, adopt best of breed A1B1C3, promptly 2a branch IBA200ppm concentration is soaked 10h, and survival rate can reach 63.30%.
(2) major part allusion quotation bamboo
Each treatment combination cuttage survival rate of major part allusion quotation bamboo low concentration group orthogonal experiment, bamboo seedling mean height, average, leading thread, every Cong Zhu seedling number, one-level radical, the hat width of cloth, ground and 8 characteristic index result of the tests of under ground portion fresh weight see Table 7.
Table 7 major part allusion quotation bamboo low concentration group cuttage result of the test table
As shown in Table 7, each treatment combination cuttage average survival of major part allusion quotation bamboo low concentration group is between 0～66.7%, average bamboo height of seedling 0.80m～1.29m, average leading thread 0.99cm～1.70cm, several 0.7～2.3 strains of every Cong Zhu seedling, 10～40 of one-level radicals, hat width of cloth 0.35m～0.96m, acrial part fresh weight 30g～484.0g, under ground portion fresh weight 50g～320g.
His-and-hers watches 7 carry out variance analysis, the results are shown in Table 8.
Table 8 major part allusion quotation bamboo low concentration group cuttage empirical risk probability (F check P value) analytical table
Table 8 result shows, major part allusion quotation bamboo low concentration group cuttage test, the different branch type survival rates and the hat width of cloth reach utmost point significant difference, between different pharmaceutical, the variable concentrations, survival rate reaches significant difference, and all the other proterties all do not have significant difference between the varying level of branch type, medicine and concentration three factors.
Major part allusion quotation bamboo high concentration group cuttage result of the test sees Table 9.As shown in Table 9, each treatment combination cuttage survival rate of major part allusion quotation bamboo high concentration group is between 0～50%, average bamboo height of seedling 1.01m～1.78m, average bamboo seedling leading thread is 0.99cm～1.7cm, several 2.0～2.7 strains of every Cong Zhu seedling, 17～56 of one-level radicals, hat width of cloth 1.03m～1.49m, acrial part fresh weight 195.33g～541.11g, under ground portion fresh weight 243.6g～560g.
His-and-hers watches 9 data are carried out variance analysis (table 10), and the result shows: survival rate, the average leading thread of bamboo seedling, every Cong Zhu seedling number, four proterties of one-level radical of the different branch of major part allusion quotation bamboo high concentration group test type all reach utmost point significant difference, and all the other all do not have significant difference.
Table 9 major part allusion quotation bamboo high concentration group cuttage result of the test data summary table
Table 10 major part allusion quotation bamboo high concentration group cuttage empirical risk probability (F check P value) analytical table
To height, low concentration experimental group survival rate index is further made LSD check (table 11, table 12), as can be known between the branch type, survival rate all reaches significant difference, IBA medicine and ABT significant difference, indifference between the concentration, two groups of result of the test explanations, major part allusion quotation bamboo spike is inserted into the motility rate primary factor and is a branch type, and the growth indexes of branch type after to cutting survival has appreciable impact, and medicament categories also has remarkable facilitation to survival rate, therefore, comprehensive comparative analysis, it is A1B1C3 and A3B1C2 that major part allusion quotation bamboo spike is inserted best of breed, promptly the branch type is 2a major branch or 1a branch, medicine is IBA, concentration 100ppm or 200ppm soak 10h, and major part allusion quotation bamboo spike is inserted into motility rate and arrives 66.7% and more than every Cong Zhu seedling mean height 1m, more than the average leading thread 1.3cm of bamboo seedling, every Cong Pingjun produces more than seedling 2 strains.
Table 11 high concentration group major part allusion quotation bamboo spike is inserted into motility rate (warp
Conversion) LSD check table
Table 12 low concentration group major part allusion quotation bamboo cuttage seeding even crown diameter (m) LSD check table
Embodiment 2 numb blue or green bamboo of support and sweet imperial bamboo branch cottage propagations
The cuttage basic process that supports numb blue or green bamboo and sweet imperial bamboo is with embodiment 1.
Choose two kinds of root-inducing promoters of 2a band pocket major branch employing IBA, ABT on the 2a maternal plant, design 100ppm, 200ppm, 1000ppm and four kinds of concentration of 2000ppm respectively, do contrast with clear water, 9 processing altogether.100ppm, 200ppm soak 10h, and 1000ppm and 2000ppm soak 10s, and completely random district group is arranged, triplicate, each sub-district cuttage 80 strain bamboo branch.
Cutting time is at the beginning of 4 months, carries out conventional field management also with embodiment 1 after inserting.
(1) the numb blue or green bamboo of support
The cuttage survival rate of the numb blue or green bamboo of different hormone-treated supports is as shown in table 13.
Each handles the cuttage survival rate of the numb blue or green bamboo of support table 13
Cottage propagation is tested minimum survival rate 3.8%, i.e. clear water contrast; The cuttage survival rate of handling with ABT1000 and ABT2000 is respectively 8.4% and 3.8%; The survival rate of handling with ABT100 and ABT200 is respectively 55.8% and 44.7%; The cuttage survival rate of handling with IBA1000 and IBA2000 is respectively 40.2% and 50.5%.Survival rate reached significant difference, Sig.=0.026 between each was handled.The LSD assay, ABT100ppm is an optimization process.
Support the slotting breeding of numb blue or green bamboo spike and handle immersion 10h with ABT100ppm, survival rate can reach 55.8%.
(2) sweet imperial bamboo
Sweet imperial bamboo spike is inserted test, and it is 4.2% that 2a major branch cuttage survival rate lowest part is put the water logging bubble in order, is treated to ABT1000ppm and ABT2000ppm and soaks 10s, and cuttage survival rate is higher, is respectively 38.3% and 39.7%.Between the different disposal, sweet each proterties of imperial bamboo does not all have significant difference, and hormone-treated minimum survival rate reaches 31.4%.
2a secondary branch cuttage survival rate, minimum 8.3%, also be the clear water immersion treatment.Be up to 35.28%, for ABT200ppm soaks 10h, each characteristic index does not also have significant difference between the different disposal, and hormone-treated minimum survival rate reaches 30.6%.
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. the cuttage breeding method of the bamboo that grows thickly comprises the steps:
1) Seedlings nursery ground is selected and whole ground: select that illumination condition is good, irrigation and drainage convenient, loose fertile mountain region yellow earth, purple soil or fluvial sand loam be as Seedlings nursery ground, deep-cut again to the garden smooth earlier, at least to turn over two rakes by two, choose most weeds tree root, per hectare applies the farmyard manure 37500kg～45000kg that becomes thoroughly decomposed, simultaneously soil is carried out pesticide and disinfectant and handle, reason limit, earthing is in order to draining;
2) HORMONE TREATMENT: cutting is soaked in the IBA solution or ABT solution of 100～200ppm, or is soaked in the IBA solution or ABT solution of 1000～2000ppm;
3) cuttage: hormone-treated cutting oblique cutting is gone into to grow seedlings in the ditch, and embedded depth 6cm～10cm stays and goes up joint branches and leaves most and basset, after blinding tracks tramping, and trickle lid grass.
2. cuttage breeding method according to claim 1, it is characterized in that, also comprise: after described step 3), carry out conventional field management, comprise in time and water, weed a garden in good time, late June to early July then in the side of bamboo seedling by going up a slope, fertilising cave apart from bamboo seedling 20～25cm cutting depth 10～15cm, N is executed in every strain behind the rain: P: K is composite fertilizer 0.1～0.2kg of 2: 1: 1, and cover with soil, carrying out weeding in 4～May and 7～August in 1 year topdresses, topdressing amount is every clump of 500～800g composite fertilizer, divide 2～3 spread manuer in holes into.
3. cuttage breeding method according to claim 1 and 2 is characterized in that step 2) in cutting at the IBA of 100～200ppm solution or ABT solution soaking 10h; Or in the IBA of 1000～2000ppm solution or ABT solution, soak 30s.
4. cuttage breeding method according to claim 1 and 2 is characterized in that, described cutting comes from biennial major branch, biennial secondary branch or annual major branch.
5. cuttage breeding method according to claim 1 and 2 is characterized in that, there are some tangible projections in the container made of bamboo, wicker, ratten, etc. portion of described cutting.
6. cuttage breeding method according to claim 1 and 2 is characterized in that, the described bamboo that grows thickly is sinocalamus latiflorus, major part allusion quotation bamboo, the numb blue or green bamboo of support or sweet imperial bamboo.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858650A (en) * | 2014-03-27 | 2014-06-18 | 乐山师范学院 | Sinocalamus affinis mcclure cutting propagation method |
| CN104641912A (en) * | 2015-02-28 | 2015-05-27 | 西南林业大学 | Out-of-reason section-burying seedling cultivation method for dendrocalamus brandisii |
| CN104782642A (en) * | 2015-03-16 | 2015-07-22 | 武汉大学 | Bamboo willow twig cutting rooting agent |
| CN107637355A (en) * | 2017-08-18 | 2018-01-30 | 李德新 | A kind of sinocalamus latiflorus method for culturing seedlings |
| CN114145142A (en) * | 2021-11-19 | 2022-03-08 | 重庆市林业科学研究院 | Cluster bamboo industrial cuttage method |
-
2011
- 2011-03-15 CN CN 201110062629 patent/CN102177803B/en not_active Expired - Fee Related
Non-Patent Citations (4)
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| 《四川林业科技》 20031231 杨桦等 撑绿杂交竹快繁育苗体系研究 第46-49页 1-6 第24卷, 第3期 2 * |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858650A (en) * | 2014-03-27 | 2014-06-18 | 乐山师范学院 | Sinocalamus affinis mcclure cutting propagation method |
| CN103858650B (en) * | 2014-03-27 | 2016-01-27 | 乐山师范学院 | The cuttage breeding method of a kind of cizu |
| CN104641912A (en) * | 2015-02-28 | 2015-05-27 | 西南林业大学 | Out-of-reason section-burying seedling cultivation method for dendrocalamus brandisii |
| CN104641912B (en) * | 2015-02-28 | 2017-01-25 | 西南林业大学 | Out-of-reason section-burying seedling cultivation method for dendrocalamus brandisii |
| CN104782642A (en) * | 2015-03-16 | 2015-07-22 | 武汉大学 | Bamboo willow twig cutting rooting agent |
| CN104782642B (en) * | 2015-03-16 | 2017-01-18 | 武汉大学 | Bamboo willow twig cutting rooting agent |
| CN107637355A (en) * | 2017-08-18 | 2018-01-30 | 李德新 | A kind of sinocalamus latiflorus method for culturing seedlings |
| CN114145142A (en) * | 2021-11-19 | 2022-03-08 | 重庆市林业科学研究院 | Cluster bamboo industrial cuttage method |
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| CN102177803B (en) | 2013-03-06 |
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