CN102099464B - 突变型dna聚合酶及相关方法 - Google Patents

突变型dna聚合酶及相关方法 Download PDF

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Publication number
CN102099464B
CN102099464B CN2008800244615A CN200880024461A CN102099464B CN 102099464 B CN102099464 B CN 102099464B CN 2008800244615 A CN2008800244615 A CN 2008800244615A CN 200880024461 A CN200880024461 A CN 200880024461A CN 102099464 B CN102099464 B CN 102099464B
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leu
glu
polymerase
lys
val
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Chinese (zh)
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CN102099464A (zh
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K·A·鲍尔
D·H·格尔范德
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
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  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2008800244615A 2007-07-13 2008-07-12 突变型dna聚合酶及相关方法 Expired - Fee Related CN102099464B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94973207P 2007-07-13 2007-07-13
US60/949,732 2007-07-13
PCT/EP2008/005714 WO2009010251A2 (en) 2007-07-13 2008-07-12 Mutant dna polymerases and related methods

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CN102099464A CN102099464A (zh) 2011-06-15
CN102099464B true CN102099464B (zh) 2013-10-30

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CN2008800244615A Expired - Fee Related CN102099464B (zh) 2007-07-13 2008-07-12 突变型dna聚合酶及相关方法

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US (1) US8026091B2 (enExample)
EP (1) EP2164955B1 (enExample)
JP (1) JP5612469B2 (enExample)
CN (1) CN102099464B (enExample)
CA (1) CA2693657C (enExample)
ES (1) ES2403591T3 (enExample)
WO (1) WO2009010251A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2706999C (en) 2007-11-28 2013-01-22 F. Hoffmann-La Roche Ag Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
EP2582802B1 (en) 2010-06-18 2016-01-13 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
JP5926246B2 (ja) * 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
JP5926247B2 (ja) 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
US10597643B2 (en) 2016-04-29 2020-03-24 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent DNA binding
US10584379B2 (en) * 2016-04-29 2020-03-10 Omniome, Inc. Method of nucleic acid sequence determination
EP3850086A1 (en) * 2018-09-13 2021-07-21 F. Hoffmann-La Roche AG Mutant dna polymerase(s) with improved strand displacement ability
CN109486788B (zh) * 2018-10-26 2021-10-22 南京市胸科医院 一种突变体dna聚合酶及其制备方法和应用
WO2021217597A1 (zh) * 2020-04-30 2021-11-04 广州达安基因股份有限公司 一种具有高扩增活性的耐热dna聚合酶突变体

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0745675A2 (en) * 1995-05-31 1996-12-04 Toyo Boseki Kabushiki Kaisha A method of amplifying nucleic acid and a reagent therefor
EP1152062A2 (en) * 2000-04-18 2001-11-07 F.Hoffmann-La Roche Ag High temperature reverse transcription using mutant DNA polymerases
CN1552869A (zh) * 1995-12-27 2004-12-08 宝酒造株式会社 新型dna聚合酶
WO2005045015A2 (en) * 2003-11-03 2005-05-19 Medical Research Council Polymerase

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
JP2006197947A (ja) * 2006-04-26 2006-08-03 Toyobo Co Ltd エキソヌクレアーゼ活性が低減された耐熱性ｄｎａポリメラーゼおよびその用途

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0745675A2 (en) * 1995-05-31 1996-12-04 Toyo Boseki Kabushiki Kaisha A method of amplifying nucleic acid and a reagent therefor
CN1552869A (zh) * 1995-12-27 2004-12-08 宝酒造株式会社 新型dna聚合酶
EP1152062A2 (en) * 2000-04-18 2001-11-07 F.Hoffmann-La Roche Ag High temperature reverse transcription using mutant DNA polymerases
WO2005045015A2 (en) * 2003-11-03 2005-05-19 Medical Research Council Polymerase

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杨广微等.噬菌体RB69 DNA聚合酶的表达、分离纯化和其聚合反应的稳态动力学研究.《中国生物化学与分子生物学报》.1999,第15卷(第6期),957-962. *

Also Published As

Publication number Publication date
WO2009010251A2 (en) 2009-01-22
US8026091B2 (en) 2011-09-27
WO2009010251A3 (en) 2009-03-05
EP2164955B1 (en) 2013-03-06
JP5612469B2 (ja) 2014-10-22
CN102099464A (zh) 2011-06-15
US20090137000A1 (en) 2009-05-28
JP2010532979A (ja) 2010-10-21
EP2164955A2 (en) 2010-03-24
WO2009010251A8 (en) 2010-04-08
ES2403591T3 (es) 2013-05-20
HK1157399A1 (en) 2012-06-29
CA2693657A1 (en) 2009-01-22
CA2693657C (en) 2013-09-10

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