CN102076846A - 具有改善的底物特异性的pqq-依赖性葡萄糖脱氢酶的新变体 - Google Patents

具有改善的底物特异性的pqq-依赖性葡萄糖脱氢酶的新变体 Download PDF

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Publication number
CN102076846A
CN102076846A CN200980124063.5A CN200980124063A CN102076846A CN 102076846 A CN102076846 A CN 102076846A CN 200980124063 A CN200980124063 A CN 200980124063A CN 102076846 A CN102076846 A CN 102076846A
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CN
China
Prior art keywords
spqqgdh
leu
glucose
gly
asn
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Pending
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CN200980124063.5A
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English (en)
Chinese (zh)
Inventor
W.韦歇尔
V.莫尔勒
M.欣德勒
R.科克
R.梅斯基斯
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Bayer Pharma AG
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Bayer Technology Services GmbH
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Publication of CN102076846A publication Critical patent/CN102076846A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01069—Gluconate 5-dehydrogenase (1.1.1.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/05—Oxidoreductases acting on the CH-OH group of donors (1.1) with a quinone or similar compound as acceptor (1.1.5)
    • C12Y101/05002—Quinoprotein glucose dehydrogenase (1.1.5.2)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN200980124063.5A 2008-06-26 2009-06-17 具有改善的底物特异性的pqq-依赖性葡萄糖脱氢酶的新变体 Pending CN102076846A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008030435A DE102008030435A1 (de) 2008-06-26 2008-06-26 Neuartige Varianten PQQ-abhängiger Glukosehydrogenase mit verbesserter Substratspezifität
DE102008030435.2 2008-06-26
PCT/EP2009/004350 WO2009156083A1 (de) 2008-06-26 2009-06-17 Neuartige varianten pqq-abhängiger glukosedehydrogenase mit verbesserter substratspezifität

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201310472347.1A Division CN103540572A (zh) 2008-06-26 2009-06-17 具有改善的底物特异性的pqq-依赖性葡萄糖脱氢酶的新变体

Publications (1)

Publication Number Publication Date
CN102076846A true CN102076846A (zh) 2011-05-25

Family

ID=40972803

Family Applications (2)

Application Number Title Priority Date Filing Date
CN200980124063.5A Pending CN102076846A (zh) 2008-06-26 2009-06-17 具有改善的底物特异性的pqq-依赖性葡萄糖脱氢酶的新变体
CN201310472347.1A Pending CN103540572A (zh) 2008-06-26 2009-06-17 具有改善的底物特异性的pqq-依赖性葡萄糖脱氢酶的新变体

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201310472347.1A Pending CN103540572A (zh) 2008-06-26 2009-06-17 具有改善的底物特异性的pqq-依赖性葡萄糖脱氢酶的新变体

Country Status (6)

Country Link
US (1) US8580547B2 (enExample)
EP (1) EP2294188A1 (enExample)
JP (1) JP2011525361A (enExample)
CN (2) CN102076846A (enExample)
DE (1) DE102008030435A1 (enExample)
WO (1) WO2009156083A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2655650B1 (en) 2010-12-20 2017-12-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
CN111073866B (zh) 2015-10-29 2024-03-19 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及其在葡萄糖检测中的应用
CN109085269B (zh) * 2018-08-15 2021-03-09 江苏省原子医学研究所 PQQ与Lys及Arg反应行为研究的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1184472C (zh) 1999-10-05 2005-01-12 松下电器产业株式会社 葡萄糖传感器
WO2002034919A1 (en) 2000-10-27 2002-05-02 Roche Diagnostics Gmbh Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
EP1367120A3 (en) 2002-05-27 2004-06-02 Toyo Boseki Kabushiki Kaisha Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability
DE10320259A1 (de) 2003-05-07 2004-11-25 Bayer Technology Services Gmbh Neuartige Glukose Dehydrogenase und ihre Herstellung
DE602004023736D1 (de) 2003-09-08 2009-12-03 Toyo Boseki Pyrrolochinolin-chinon (pqq)-abhängige glucosedehydrogenase-modifikation mit ausgezeichneter substratspezifität
JP2007043983A (ja) * 2005-08-11 2007-02-22 Toyobo Co Ltd 基質特異性に優れた改変型ピロロキノリンキノン依存性グルコースデヒドロゲナーゼ
JP2007000020A (ja) * 2005-06-21 2007-01-11 Toyobo Co Ltd 基質特異性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
WO2006085509A1 (ja) * 2005-02-08 2006-08-17 Toyo Boseki Kabushiki Kaisha 基質特異性に優れた改変型ピロロキノリンキノン依存性グルコースデヒドロゲナーゼ
JP2006217810A (ja) * 2005-02-08 2006-08-24 Toyobo Co Ltd 基質特異性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体

Also Published As

Publication number Publication date
WO2009156083A1 (de) 2009-12-30
CN103540572A (zh) 2014-01-29
US20110236920A1 (en) 2011-09-29
JP2011525361A (ja) 2011-09-22
EP2294188A1 (de) 2011-03-16
US8580547B2 (en) 2013-11-12
DE102008030435A1 (de) 2010-01-07

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