CN101945999B - 使用稳定辅酶的脱氢酶稳定作用 - Google Patents

使用稳定辅酶的脱氢酶稳定作用 Download PDF

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Publication number
CN101945999B
CN101945999B CN200980105640.6A CN200980105640A CN101945999B CN 101945999 B CN101945999 B CN 101945999B CN 200980105640 A CN200980105640 A CN 200980105640A CN 101945999 B CN101945999 B CN 101945999B
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enzyme
coenzyme
nad
stabilized
stabilizing
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Chinese (zh)
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CN101945999A (zh
Inventor
D·海因德尔
C·霍恩
C·加斯勒迪切
J·霍内斯
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01005—L-Amino-acid dehydrogenase (1.4.1.5)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200980105640.6A 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用 Expired - Fee Related CN101945999B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08003054A EP2093284A1 (de) 2008-02-19 2008-02-19 Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
EP08003054.7 2008-02-19
PCT/EP2009/001206 WO2009103540A1 (de) 2008-02-19 2009-02-19 Stabilisierung von dehydrogenasen mit stabilen coenzymen

Related Child Applications (1)

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CN201410680334.8A Division CN104450659A (zh) 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用

Publications (2)

Publication Number Publication Date
CN101945999A CN101945999A (zh) 2011-01-12
CN101945999B true CN101945999B (zh) 2016-06-08

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Family Applications (3)

Application Number Title Priority Date Filing Date
CN200980105640.6A Expired - Fee Related CN101945999B (zh) 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用
CN201410680334.8A Pending CN104450659A (zh) 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用
CN2010800085457A Pending CN102325896A (zh) 2008-02-19 2010-02-12 干化学层中具有低活性的酶的快速反应动力学

Family Applications After (2)

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CN201410680334.8A Pending CN104450659A (zh) 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用
CN2010800085457A Pending CN102325896A (zh) 2008-02-19 2010-02-12 干化学层中具有低活性的酶的快速反应动力学

Country Status (15)

Country Link
US (2) US9896666B2 (https=)
EP (2) EP2093284A1 (https=)
JP (2) JP5758128B2 (https=)
KR (3) KR101381004B1 (https=)
CN (3) CN101945999B (https=)
AR (1) AR070436A1 (https=)
AU (1) AU2009216908B9 (https=)
BR (1) BRPI0907839A2 (https=)
CA (1) CA2721718C (https=)
HK (1) HK1208703A1 (https=)
MX (1) MX2010008920A (https=)
RU (1) RU2499834C2 (https=)
TW (1) TWI482860B (https=)
WO (1) WO2009103540A1 (https=)
ZA (1) ZA201006638B (https=)

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EP2093284A1 (de) 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
EP2226007A1 (de) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Testelementmagazin mit abgedeckten Testfeldern
EP2226008A1 (de) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Verfahren zur Herstellung eines analytischen Magazins
EP2398388B1 (de) 2009-02-19 2020-04-08 Roche Diabetes Care GmbH Platzsparende magazinierung analytischer hilfsmittel
KR20130127555A (ko) * 2009-02-19 2013-11-22 에프. 호프만-라 로슈 아게 건식 화학 층에서 낮은 활성을 갖는 효소의 고속 반응 속도
EP2398909B1 (de) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
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EP2636750A1 (en) * 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
CN107024474B (zh) 2012-06-22 2020-11-06 霍夫曼-拉罗奇有限公司 用于检测体液中的分析物的方法和器件
US8921061B2 (en) * 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Reagent materials and associated test elements
US8920628B2 (en) 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Systems and methods for multiple analyte analysis
CN104870982B (zh) 2012-12-20 2019-02-15 霍夫曼-拉罗奇有限公司 用于评估医学测量曲线的方法
KR101750638B1 (ko) 2012-12-20 2017-06-23 에프. 호프만-라 로슈 아게 체액의 샘플을 분석하는 방법
EP2781919A1 (en) 2013-03-19 2014-09-24 Roche Diagniostics GmbH Method / device for generating a corrected value of an analyte concentration in a sample of a body fluid
WO2014180939A1 (en) 2013-05-08 2014-11-13 Roche Diagnostics Gmbh Stabilization of enzymes by nicotinic acid
HK1213296A1 (zh) * 2013-06-04 2016-06-30 豪夫迈‧罗氏有限公司 用於fret的新化合物及与其相关的方法
EP3063169B1 (en) 2013-10-29 2018-10-10 F. Hoffmann-La Roche AG Nano-enzyme containers for test elements
WO2015078899A1 (en) 2013-11-27 2015-06-04 Roche Diagnostics Gmbh Composition comprising up-converting phosphors for detecting an analyte
EP2927319A1 (en) 2014-03-31 2015-10-07 Roche Diagnostics GmbH High load enzyme immobilization by crosslinking
CA2945612C (en) 2014-04-14 2019-09-10 F. Hoffmann-La Roche Ag Phenazinium mediators
EP3183246B1 (en) 2014-08-22 2020-09-23 Roche Diagnostics GmbH Redoxindicators
CN107779459B (zh) * 2016-08-31 2021-06-08 安琪酵母股份有限公司 葡萄糖脱氢酶dna分子、载体和菌株及应用
CA3035874C (en) 2016-10-05 2025-09-09 F. Hoffmann-La Roche Ag DETECTION REAGENTS AND ELECTRODE ARRANGEMENTS FOR MULTI-ANALYTICAL DIAGNOSTIC TEST ELEMENTS, AS WELL AS THEIR METHODS OF USE
EP3339431A1 (en) 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
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WO1998033936A1 (en) * 1997-02-04 1998-08-06 Specialty Assays, Inc. The use of nadph and nadh analogs in the measurement of enzyme activities and metabolites
WO2001049247A2 (en) * 1999-12-30 2001-07-12 Specialty Assays, Inc. Assays for analytes and enzymes practiced in the presence of nad and nadp analogues, and related assay kits
US20070026476A1 (en) * 2005-07-28 2007-02-01 Dieter Heindl Compounds, methods, complexes, apparatuses and uses relating to stabile forms of nad/nadh
WO2007012494A1 (de) * 2005-07-28 2007-02-01 F.Hoffmann-La Roche Ag Stabile nad/nadh-derivate

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Publication number Publication date
JP6113405B2 (ja) 2017-04-12
TWI482860B (zh) 2015-05-01
EP2245152B1 (de) 2019-05-01
US20110143416A1 (en) 2011-06-16
BRPI0907839A2 (pt) 2015-08-11
RU2499834C2 (ru) 2013-11-27
HK1208703A1 (en) 2016-03-11
KR20120134142A (ko) 2012-12-11
US20180265849A1 (en) 2018-09-20
JP2011514153A (ja) 2011-05-06
CA2721718C (en) 2017-11-28
AU2009216908B2 (en) 2012-04-19
EP2093284A1 (de) 2009-08-26
KR101381004B1 (ko) 2014-04-08
ZA201006638B (en) 2011-06-29
AU2009216908B9 (en) 2012-07-05
AR070436A1 (es) 2010-04-07
AU2009216908A1 (en) 2009-08-27
MX2010008920A (es) 2010-09-07
JP2012517816A (ja) 2012-08-09
RU2010138610A (ru) 2012-03-27
CN102325896A (zh) 2012-01-18
JP5758128B2 (ja) 2015-08-05
WO2009103540A1 (de) 2009-08-27
TW200951222A (en) 2009-12-16
CA2721718A1 (en) 2009-08-27
CN104450659A (zh) 2015-03-25
US11220674B2 (en) 2022-01-11
KR20130081300A (ko) 2013-07-16
KR20100118995A (ko) 2010-11-08
US9896666B2 (en) 2018-02-20
CN101945999A (zh) 2011-01-12
EP2245152A1 (de) 2010-11-03

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