CN101919494A - Complex microorganism additive as well as preparation method and application thereof - Google Patents
Complex microorganism additive as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN101919494A CN101919494A CN2010102968199A CN201010296819A CN101919494A CN 101919494 A CN101919494 A CN 101919494A CN 2010102968199 A CN2010102968199 A CN 2010102968199A CN 201010296819 A CN201010296819 A CN 201010296819A CN 101919494 A CN101919494 A CN 101919494A
- Authority
- CN
- China
- Prior art keywords
- complex microorganism
- bacterial classification
- microorganism additive
- yeast
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a complex microorganism additive as well as a preparation method and application thereof, belonging to the biological material technical field of application of a microecological modulator in cultivating domestic animals. The preparation method thereof comprises the following steps: taking 30-60% of Saccharomyces cervisiae, 5-30% of Candida utilis, 5-30% of Rhodopseudom onas Palustris, 5-30% of Lactobacillus plantarum, 5-40% of Leatobacillus acidophilus and 5-25% of Bacillus subtilis; and sequentially carrying out primary bacteria strain culture, secondary fermentation and tertiary fermentation on the bacteria strains so that the bacterial number in fermentation liquor reaches 8-10*108/mL, thus obtaining the complex microorganism additive 808. Long-term on-site experiments prove that the product has reasonable formula, stable efficacy, disease prevention and resistance, improved quality and capability of promoting growth of animals and improving yield of animal products, survival rate and feed conversion rate.
Description
Technical field
The present invention relates to a kind of complex microorganism additive 808 and preparation method thereof and application, belong to the technical field of biological materials that plant biotechnology regulator is used in proportion of crop planting.
Background technology
The initial definition of feed addictive is a feed supplement, fills up the deficiency of nutritional labeling in the feed to add compositions such as vitamin and inorganic salts, the nutrition of balanced feed, and obtain good feeding effect.
Along with intensify the increase with its feeding density of further developing of dead sample of livestock and poultry, the generation of various livestock and poultries is more and more frequent, and most livestock and poultry of culturing all are in sub-health state.Once widely used medicine not only makes pathogenic microorganism produce the serious resistance to the action of a drug, also causes the serious problems of livestock products medicament residue, and development goes down to influence human beings'health.
Probiotics arises at the historic moment under this historical background, for the sustainable development of animal husbandry provide one effectively, approach reliably.Adopt the biotechnology composite fermentation, utilize multiple microbial interaction, form the system of an ecological balance.
Summary of the invention
Problem to be solved by this invention is to adopt multiple strain preparation complex micro organism fungicide, utilizes this complex micro organism fungicide to make the feed grade complex microorganism additive and is applied in livestock and poultry breeding industry.Enter in the livestock and poultry animal body, form dominant population, participate in the balance of enteric microorganism, improve the efficiency of feed utilization and the speed of growth of animal, improve the animal body non-specific immune function, disease resistance.
Technical solution of the present invention is as follows: a kind of complex microorganism additive 808 employing brewer's yeasts (
Saccharomyces cerevisiae) ATCC No.9763, candida utili (
Candida utilis) ATCC No.22023, Rhodopseudomonas palustris (
Rhodopseudom onas Palustris) ATCCNo.33872, Lactobacillus plantarum (
Lactobacillus plantarum) ATCC No.8014, lactobacillus acidophilus (
Leatobacillus acidophilus) ATCC No.11073, bacillus subtilis (
Bacillus subtilis) ATCC No.6633 prepares bacterial classification; The mass percent of described bacterial classification is as follows: brewer's yeast 30 ~ 60%, candida utili 5 ~ 30%, Rhodopseudomonas palustris 5 ~ 30%, Lactobacillus plantarum 5 ~ 30%, lactobacillus acidophilus 5 ~ 40%, bacillus subtilis 5 ~ 25%.
The preparation process of described a kind of complex microorganism additive 808 is as follows:
1. inoculation: described each bacterial classification is connected on the slant medium.The brewer's yeast cultivation temperature is 30 ℃, and incubation time is 6～48h; 25～30 ℃ of candida utili cultivation temperature, incubation time are 6～48h; The Rhodopseudomonas palustris cultivation temperature is 25～30 ℃, and incubation time is 6～48h; 25～30 ℃ of Lactobacillus plantarum cultivation temperature, incubation time are 6～48h; The lactobacillus acidophilus cultivation temperature is 27～37 ℃, and incubation time is 6～48h; The bacillus subtilis cultivation temperature is 27～37 ℃, and incubation time is 6～48h;
2. first class inoculum is cultivated: described bacterial classification is inoculated in respectively in the 50mL conical flask that fills fluid nutrient medium, and medium pH is 6.0～8.0, and conical flask is put into the shaking table vibration, and temperature is 30 ℃, vibration 24～48h; Employing blood counting chamber counting, bacterial classification quantity reaches 10 respectively in the bacterium liquid
8Individual/mL;
3. second order fermentation: described bacterial classification is inoculated in respectively in the 1000mL conical flask that fills fluid nutrient medium, and medium pH 3 ~ 6 is put into the shaking table vibration, and temperature is 30 ℃, vibration 48～72h; Make bacterial classification quantity reach 10 respectively
8Individual/as more than the mL, each bacterial classification to be mixed by the mass percent of described bacterial classification;
4. three grade fermemtation: mixed bacterium liquid is inoculated in the fermentation tank that fills the composite bacteria agent capable culture medium, composite bacteria agent capable medium pH 3 ~ 6, inoculum concentration is 1～5%, 30 ℃ cultivated 4～7 days down, made that the bacterium number reaches 8～10 * 10 in the zymotic fluid
8Individual/mL, promptly obtain described complex microbial inoculum.
Described Liquid Culture based formulas is:
1. the Liquid Culture based formulas of brewer's yeast employing is: casein peptone 15g/L, yeast 25g/L, glucose 30 g/L, KH
2PO
42.4g/L, K
2HPO
43H
2O 16.34 g/L;
2. the Liquid Culture based formulas of producing the employing of Ruan's Candida is: brewer's wort to the concentration that hops is removed in dilution is 12Brix, agar 15 g, autoclaving 30 minutes;
3. the Liquid Culture based formulas of Rhodopseudomonas palustris employing is: ammonium chloride 1.5g, potassium dihydrogen phosphate 1.5g, epsom salt 0.8g, sodium chloride 4.0g, sodium acid carbonate 1.8g, calcium chloride 0.01g, VB0.1g, dusty yeast 1.0 g, sodium acetate 4.0g, micro-1.0mL/L comprise (Iron trichloride hexahydrate 5.0g, white vitriol 1.0g, cupric sulfate pentahydrate 0.05g, six water chlorinations
Cobalt 0.5g, boric acid 1.0g, tetrahydrate manganese chloride 0.5g), adding acetate and regulate pH, water is an amount of;
4. the Liquid Culture based formulas of Lactobacillus plantarum, lactobacillus acidophilus employing is: MC peptone 10g, peptone 10g,
Yeast extract 5g, glucose 5g, Tween 80 1g, K
2HPO
42g, sodium acetate 5g, citric acid diamines 2g, MgSO
47H
2O 0.2g, MnSO
4H
2O 0.05g, distilled water 1000ml;
5. the Liquid Culture based formulas of bacillus subtilis employing is: beef extract 5g, peptone 10g, Nacl 3g, pH 7.0.
Described composite bacteria agent capable culture medium prescription is: honey 1～5g/100mL, sucrose 2～10g/100mL, peptone 0.1～0.5g/100mL, KH
2PO
40.1～0.5g/100mL, K
2HPO
40.5～2g/100mL, MgSO
47H
2All the other are distilled water for O 0.5～2g/100mL, Nacl 0.5～2g/100mL.
Described complex microorganism additive 808 in livestock and poultry animal is cultured as feed addictive.
The invention has the beneficial effects as follows: complex micro organism fungicide adopts brewer's yeast, candida utili, Rhodopseudomonas palustris, Lactobacillus plantarum, lactobacillus acidophilus, bacillus subtilis to prepare bacterial classification.Above bacterial classification is cultivated through first class inoculum respectively, and two, three grade fermemtation, make that the bacterium number reaches 8～10 * 10 in the zymotic fluid
8Individual/mL, promptly get complex microorganism additive 808.This product is through checking on the spot for a long time, and prescription is reasonable, and stable effect can promote growth of animal, improves animal product output, improves survival rate, and disease resistance is improved food conversion ratio, and improves product quality.
The specific embodiment
The present invention adopt brewer's yeast (
Saccharomyces cerevisiae) ATCC No.9763, candida utili (
Candida utilis) ATCC No.22023, Rhodopseudomonas palustris (
Rhodopseudom onas Palustris) ATCCNo.33872, Lactobacillus plantarum (
Lactobacillus plantarum) ATCC No.8014, lactobacillus acidophilus (
Leatobacillus acidophilus) ATCC No.11073, bacillus subtilis (
Bacillus subtilis) ATCC No.6633 prepares bacterial classification, produces complex microorganism additive 808.
The mass percent of bacterial classification is: brewer's yeast 30 ~ 60%, candida utili 5 ~ 30%, Rhodopseudomonas palustris 5 ~ 30%, Lactobacillus plantarum 5 ~ 30%, lactobacillus acidophilus 5 ~ 40%, bacillus subtilis 5 ~ 25%.
The common prescription table
According to the complex microorganism additive 808 that above-mentioned formula table and preparation method obtain, bacterium is counted content and reaches 9 * 10
8Individual/mL.
Embodiment 1:
The complex microorganism additive 808(that adopts the present invention's preparation is hereinafter to be referred as 808) effect test in piglet is cultured:
1. for the examination material: complex microorganism additive 808
2. subjects: Baoshan aggie selects on the pig farm wean of 45 ages in days to be about 16 of 2 yuan of assorted piglets.
3. test grouping: evenly divide into groups according to strong and weak size matching, 8 every group, the equal body weight 12.79kg of test group head, the equal body weight 13.31kg of control group head.
Test requirements document: the basal feed preparation of test group and control group is identical.The test group diet adds complex microorganism additive 808 fermentation 4-6 talentes and feeds.
4. test method:
Test group: per 0.1 ton of feed uses complex microorganism additive 808 100mL, adds the 30kg clear water.
Control group: basal feed adds " pig doctor " premix, feeds.
Carry out 5 days feed earlier, enter 32 days formal test phases then.
Table 1 basal feed is formed and nutritional labeling
| Ration ingredient | Form (%) | Nutritional labeling | Form (%) |
| Maize | 60 | Digestible energy | 3.36MJ/kg |
| Dregs of beans | 20 | Crude protein | 17.38 |
| Wheat bran | 5 | Calcium | 0.55 |
| Rice bran | 5 | Phosphorus | 0.52 |
| Rapeseed cake | 6 | ? | ? |
| Fish meal | 2 | ? | ? |
| Premix | 2 | ? | ? |
The test group fermented feed of feeding, control group fed wet-mixing material is fed 4 every day, and two groups are all adopted the restriction mode (most probably full) of feeding, freely drink water.
5. test results and analysis
Test group daily gain 348g, control group daily gain 333g.The piglet feed intake of test group is few, drowsiness, and the ight soil amount is few and stink is little; The motion of control group piglet hobby, respiratory disease appears in minority.This shows, use the complex microorganism additive 808 fermented feeds piglet of feeding, improve the utilization rate of feed, reduce production costs relatively, effectively prevented stomach and intestine and the respiratory disease of piglet.
Embodiment 2:
The complex microorganism additive 808(that adopts the present invention's preparation is hereinafter to be referred as 808) effect test in milk cattle cultivating:
1. test material:
For trying milk cow: the lactation holstein cow of healthy no disease
2. test method:
Two testing sites are established in test, and each testing site 10 cow head is divided into two groups, the test group complex microorganism additive 808 of feeding, and another organizes contrast.
First testing site, feed adds once a day.
Every day is divided morning, noon, three feeds interpolations in evening in second testing site.
3. test period is 30 days.5 day preliminary trial period,, the formal test phase is 25 days.
4. result of the test:
Test preceding 10 days test group and improve 8% than the control group output of milk.Back average comparison in 15 days improves 13.4% according to group.It is remarkable that milk cow increases the milk effect.The daily ration utilization rate improves, and production cost reduces.
Claims (5)
1. complex microorganism additive is characterized in that: adopt brewer's yeast (
Saccharomyces cerevisiae) ATCC No.9763, candida utili (
Candida utilis) ATCC No.22023, Rhodopseudomonas palustris (
Rhodopseudom onas Palustris) ATCCNo.33872, Lactobacillus plantarum (
Lactobacillus plantarum) ATCC No.8014, lactobacillus acidophilus (
Leatobacillus acidophilus) ATCC No.11073, bacillus subtilis (
Bacillus subtilis) ATCC No.6633 prepares bacterial classification; The mass percent of described bacterial classification is as follows: brewer's yeast 30 ~ 60%, candida utili 5 ~ 30%, Rhodopseudomonas palustris 5 ~ 30%, Lactobacillus plantarum 5 ~ 30%, lactobacillus acidophilus 5 ~ 40%, bacillus subtilis 5 ~ 25%.
2. the preparation method of a kind of complex microorganism additive according to claim 1, it is characterized in that: the preparation process of described complex microorganism additive is as follows:
1. inoculation: described each bacterial classification is connected on the slant medium.The brewer's yeast cultivation temperature is 30 ℃, and incubation time is 6～48h; 25～30 ℃ of candida utili cultivation temperature, incubation time are 6～48h; The Rhodopseudomonas palustris cultivation temperature is 25～30 ℃, and incubation time is 6～48h; 25～30 ℃ of Lactobacillus plantarum cultivation temperature, incubation time are 6～48h; The lactobacillus acidophilus cultivation temperature is 27～37 ℃, and incubation time is 6～48h; The bacillus subtilis cultivation temperature is 27～37 ℃, and incubation time is 6～48h;
2. first class inoculum is cultivated: described bacterial classification is inoculated in respectively in the 50mL conical flask that fills fluid nutrient medium, and medium pH is 6.0～8.0, and conical flask is put into the shaking table vibration, and temperature is 30 ℃, vibration 24～48h; Employing blood counting chamber counting, bacterial classification quantity reaches 10 respectively in the bacterium liquid
8Individual/mL;
3. second order fermentation: described bacterial classification is inoculated in respectively in the 1000mL conical flask that fills fluid nutrient medium, and medium pH 3 ~ 6 is put into the shaking table vibration, and temperature is 30 ℃, vibration 48～72h; Make bacterial classification quantity reach 10 respectively
8Individual/as more than the mL, each bacterial classification to be mixed by the mass percent of described bacterial classification;
4. three grade fermemtation: mixed bacterium liquid is inoculated in the fermentation tank that fills the composite bacteria agent capable culture medium, composite bacteria agent capable medium pH 3 ~ 6, inoculum concentration is 1～5%, 30 ℃ cultivated 4～7 days down, made that the bacterium number reaches 8～10 * 10 in the zymotic fluid
8Individual/mL, promptly obtain described complex microbial inoculum.
3. the preparation method of a kind of complex microorganism additive according to claim 2, it is characterized in that: described Liquid Culture based formulas is:
1. the Liquid Culture based formulas of brewer's yeast employing is: casein peptone 15g/L, yeast 25g/L, glucose 30 g/L, KH
2PO
42.4g/L, K
2HPO
43H
2O 16.34 g/L;
2. the Liquid Culture based formulas of producing the employing of Ruan's Candida is: brewer's wort to the concentration that hops is removed in dilution is 12Brix, agar 15 g, autoclaving 30 minutes;
3. the Liquid Culture based formulas of Rhodopseudomonas palustris employing is: ammonium chloride 1.5g, potassium dihydrogen phosphate 1.5g, epsom salt 0.8g, sodium chloride 4.0g, sodium acid carbonate 1.8g, calcium chloride 0.01g, VB0.1g, dusty yeast 1.0 g, sodium acetate 4.0g, trace element 1.0mL/L comprises (Iron trichloride hexahydrate 5.0g, white vitriol 1.0g, cupric sulfate pentahydrate 0.05g, CoCL2 0.5g, boric acid 1.0g, tetrahydrate manganese chloride 0.5g), add acetate and regulate pH, water is an amount of;
4. the Liquid Culture based formulas of Lactobacillus plantarum, lactobacillus acidophilus employing is: MC peptone 10g, peptone 10g, yeast extract 5g, glucose 5g, Tween 80 1g, K
2HPO
42g, sodium acetate 5g, citric acid diamines 2g, MgSO
47H
2O 0.2g, MnSO
4H
2O 0.05g, distilled water 1000ml;
5. the Liquid Culture based formulas of bacillus subtilis employing is: beef extract 5g, peptone 10g, Nacl 3g, pH 7.0.
4. the preparation method of a kind of complex microorganism additive according to claim 2, it is characterized in that: described composite bacteria agent capable culture medium prescription is: honey 1～5g/100mL, sucrose 2～10g/100mL, peptone 0.1～0.5g/100mL, KH
2PO
40.1～0.5g/100mL, K
2HPO
40.5～2g/100mL, MgSO
47H
2All the other are distilled water for O 0.5～2g/100mL, Nacl 0.5～2g/100mL.
5. the application of complex microorganism additive according to claim 1 is characterized in that: described complex microorganism additive in livestock and poultry animal is cultured as feed addictive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102968199A CN101919494B (en) | 2010-09-30 | 2010-09-30 | Complex microorganism additive as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102968199A CN101919494B (en) | 2010-09-30 | 2010-09-30 | Complex microorganism additive as well as preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101919494A true CN101919494A (en) | 2010-12-22 |
| CN101919494B CN101919494B (en) | 2012-06-06 |
Family
ID=43334932
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102968199A Expired - Fee Related CN101919494B (en) | 2010-09-30 | 2010-09-30 | Complex microorganism additive as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101919494B (en) |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103082156A (en) * | 2013-02-22 | 2013-05-08 | 内蒙古农业大学 | Ruminant compound microecological preparation and application thereof |
| CN103333824A (en) * | 2013-06-21 | 2013-10-02 | 大连三科生物工程有限公司 | Microbial organic fertilizer and preparation method thereof |
| CN103392917A (en) * | 2013-07-29 | 2013-11-20 | 湖南圣雅凯生物科技有限公司 | Composite feed additive preparation technology based on gynostemma pentaphylla |
| CN103497906A (en) * | 2013-08-02 | 2014-01-08 | 国家海洋局第三海洋研究所 | Microecological preparation, preparation method, and applications thereof |
| CN103636967A (en) * | 2013-11-23 | 2014-03-19 | 张晨 | Composite microecological preparation for broiler chickens |
| CN103652329A (en) * | 2013-12-18 | 2014-03-26 | 大连金砣水产食品有限公司 | Compound probiotic biological agent |
| CN103861447A (en) * | 2012-12-14 | 2014-06-18 | 哈尔滨科艺生物环保科技有限责任公司 | Breeding site cleaning agent and preparation method thereof |
| CN104012787A (en) * | 2014-05-16 | 2014-09-03 | 张海员 | Method for preparing corn straw coarse feed by microbial beneficial living bacteria |
| CN104605154A (en) * | 2015-02-13 | 2015-05-13 | 山东省农业科学院畜牧兽医研究所 | Fermented feed |
| CN105211599A (en) * | 2015-11-18 | 2016-01-06 | 乌鲁木齐格莱克工程技术有限公司 | A kind of ruminant chitosan oligosaccharide composite probiotics preparations |
| CN105454731A (en) * | 2015-12-28 | 2016-04-06 | 上海创博生态工程有限公司 | Microorganism feed dedicated for Trachidermus fasciatus Heckel and preparation method thereof |
| CN107056524A (en) * | 2017-01-25 | 2017-08-18 | 北京绿穑生物科技有限责任公司 | A kind of novel microbial nursery nutrient solution and its preparation method and application |
| CN108208325A (en) * | 2018-01-19 | 2018-06-29 | 深圳市帝之道科技贸易有限公司 | A kind of probiotics composite mineral matter additive |
| CN108522564A (en) * | 2018-05-15 | 2018-09-14 | 何治伟 | A kind of preparation method of rice herbicide |
| CN109593679A (en) * | 2018-12-26 | 2019-04-09 | 大连雪龙都市农业有限公司 | A kind of nanometer selenium microbial bacterial agent and the preparation method and application thereof |
| CN111187739A (en) * | 2020-02-14 | 2020-05-22 | 广东中科无抗养殖科技有限公司 | Compound microbial preparation and culture method and application thereof |
| CN112715750A (en) * | 2020-12-31 | 2021-04-30 | 江西瑞博特生物科技有限公司 | Compound microbial agent and feed additive for livestock and poultry breeding and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103445020A (en) * | 2013-08-23 | 2013-12-18 | 福建大用生态农业综合发展有限公司 | Fermented feed for feeding pigs |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006020581A (en) * | 2004-07-08 | 2006-01-26 | Cdom:Kk | Pet food and method for producing the same |
| CN101278702A (en) * | 2007-04-05 | 2008-10-08 | 北京大北农科技集团有限责任公司 | Novel microbial feed additive and method of producing the same |
| CN101530161A (en) * | 2008-03-10 | 2009-09-16 | 北京大北农科技集团股份有限公司 | Novel feed additive and preparation method thereof |
| CN101530165A (en) * | 2009-04-23 | 2009-09-16 | 上海谱莱生物技术有限公司 | Animal feed composite microecologic agent |
-
2010
- 2010-09-30 CN CN2010102968199A patent/CN101919494B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006020581A (en) * | 2004-07-08 | 2006-01-26 | Cdom:Kk | Pet food and method for producing the same |
| CN101278702A (en) * | 2007-04-05 | 2008-10-08 | 北京大北农科技集团有限责任公司 | Novel microbial feed additive and method of producing the same |
| CN101530161A (en) * | 2008-03-10 | 2009-09-16 | 北京大北农科技集团股份有限公司 | Novel feed additive and preparation method thereof |
| CN101530165A (en) * | 2009-04-23 | 2009-09-16 | 上海谱莱生物技术有限公司 | Animal feed composite microecologic agent |
Non-Patent Citations (3)
| Title |
|---|
| 《家禽用药500问》 20060131 刘高升 饲料药物添加剂 第249-250页 1-5 , 1 * |
| 《畜牧与饲料科学》 20091231 王金合 微生态饲料添加剂及其在畜禽生产中的应用研究现状 第36-38页 1-5 第30卷, 第2期 2 * |
| 《陕西农业科学》 20021231 赵献军 微生物饲料添加剂 第18-21页 1-5 , 第2期 2 * |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103861447A (en) * | 2012-12-14 | 2014-06-18 | 哈尔滨科艺生物环保科技有限责任公司 | Breeding site cleaning agent and preparation method thereof |
| CN103861447B (en) * | 2012-12-14 | 2015-09-30 | 哈尔滨科艺生物环保科技有限责任公司 | A kind of cultivation place cleanser and preparation method thereof |
| CN103082156B (en) * | 2013-02-22 | 2015-10-14 | 内蒙古农业大学 | A kind of ruminant compound microecological preparation and application thereof |
| CN103082156A (en) * | 2013-02-22 | 2013-05-08 | 内蒙古农业大学 | Ruminant compound microecological preparation and application thereof |
| CN103333824A (en) * | 2013-06-21 | 2013-10-02 | 大连三科生物工程有限公司 | Microbial organic fertilizer and preparation method thereof |
| CN103333824B (en) * | 2013-06-21 | 2015-01-21 | 大连三科生物工程有限公司 | Microbial organic fertilizer and preparation method thereof |
| CN103392917A (en) * | 2013-07-29 | 2013-11-20 | 湖南圣雅凯生物科技有限公司 | Composite feed additive preparation technology based on gynostemma pentaphylla |
| CN103497906A (en) * | 2013-08-02 | 2014-01-08 | 国家海洋局第三海洋研究所 | Microecological preparation, preparation method, and applications thereof |
| CN103636967A (en) * | 2013-11-23 | 2014-03-19 | 张晨 | Composite microecological preparation for broiler chickens |
| CN103636967B (en) * | 2013-11-23 | 2015-04-01 | 德州市畜牧协会 | Composite microecological preparation for broiler chickens |
| CN103652329A (en) * | 2013-12-18 | 2014-03-26 | 大连金砣水产食品有限公司 | Compound probiotic biological agent |
| CN104012787A (en) * | 2014-05-16 | 2014-09-03 | 张海员 | Method for preparing corn straw coarse feed by microbial beneficial living bacteria |
| CN104605154A (en) * | 2015-02-13 | 2015-05-13 | 山东省农业科学院畜牧兽医研究所 | Fermented feed |
| CN104605154B (en) * | 2015-02-13 | 2017-07-25 | 山东省农业科学院畜牧兽医研究所 | A kind of fermented feed |
| CN105211599A (en) * | 2015-11-18 | 2016-01-06 | 乌鲁木齐格莱克工程技术有限公司 | A kind of ruminant chitosan oligosaccharide composite probiotics preparations |
| CN105454731A (en) * | 2015-12-28 | 2016-04-06 | 上海创博生态工程有限公司 | Microorganism feed dedicated for Trachidermus fasciatus Heckel and preparation method thereof |
| CN107056524A (en) * | 2017-01-25 | 2017-08-18 | 北京绿穑生物科技有限责任公司 | A kind of novel microbial nursery nutrient solution and its preparation method and application |
| CN108208325A (en) * | 2018-01-19 | 2018-06-29 | 深圳市帝之道科技贸易有限公司 | A kind of probiotics composite mineral matter additive |
| CN108522564A (en) * | 2018-05-15 | 2018-09-14 | 何治伟 | A kind of preparation method of rice herbicide |
| CN109593679A (en) * | 2018-12-26 | 2019-04-09 | 大连雪龙都市农业有限公司 | A kind of nanometer selenium microbial bacterial agent and the preparation method and application thereof |
| CN111187739A (en) * | 2020-02-14 | 2020-05-22 | 广东中科无抗养殖科技有限公司 | Compound microbial preparation and culture method and application thereof |
| CN112715750A (en) * | 2020-12-31 | 2021-04-30 | 江西瑞博特生物科技有限公司 | Compound microbial agent and feed additive for livestock and poultry breeding and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101919494B (en) | 2012-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101919494B (en) | Complex microorganism additive as well as preparation method and application thereof | |
| CN101999525B (en) | Composite probiotics feed additive | |
| CN102488084B (en) | Method for preparing protein feed by fermentation on straw and cake using aerobic mixed bacteria | |
| CN102228138B (en) | Microbiological additive for ruminants and preparation technology thereof | |
| CN102669483B (en) | Mixed plant protein fish feed as well as preparation method and application thereof | |
| CN101019609A (en) | Solid fermentation process for producing natto bacillus feed additive with bean dregs | |
| CN104012803B (en) | A kind of preparation method of the fermented feed that can prevent mastitis for milk cows | |
| CN103652367B (en) | A kind of ruminant composite microorganism type forage additives and preparation method thereof | |
| CN100563671C (en) | Fox immunity enhancer | |
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| CN101658243B (en) | Microbiological feed additive for adjusting energy metabolism impairment of flocks and herds during perinatal period | |
| CN101209088A (en) | Microorganism polyzyme additive agent for improving pigling growth and development | |
| CN104872384B (en) | Needle mushroom foot feed addictive | |
| CN104664169A (en) | Biological enhancer for ruminant feeds, and preparation method for biological enhancer | |
| CN106071143B (en) | A kind of granular pattern probiotics and preparation method thereof that ruminant production performance can be improved | |
| CN102165995B (en) | Composite premix for pig | |
| CN105981927A (en) | Bioactive companion pellet feed suitable for weaning piglets and preparation method thereof | |
| CN103783290B (en) | A kind of reduction cholesterol of poultry egg heavy duty detergent feed addictive and preparation method | |
| CN106234755A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with bagasse | |
| CN102210454B (en) | Microbial compound preparation and preparation method thereof | |
| CN110192595A (en) | A kind of composite microorganism additive agent for milk cattle feed stuff and its preparation method and application | |
| CN101690541B (en) | Method for preparing feed protein from microbial fermented silkworms | |
| CN109486724A (en) | A kind of synchronization preparation process and its feed applications mixing probiotics leaven | |
| CN106035988B (en) | Production method of arginine active peptide powder for livestock and poultry | |
| CN102940145A (en) | Preparation method of fermentation aquatic health care feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120606 Termination date: 20210930 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |