CN105211599A - A kind of ruminant chitosan oligosaccharide composite probiotics preparations - Google Patents
A kind of ruminant chitosan oligosaccharide composite probiotics preparations Download PDFInfo
- Publication number
- CN105211599A CN105211599A CN201510797342.5A CN201510797342A CN105211599A CN 105211599 A CN105211599 A CN 105211599A CN 201510797342 A CN201510797342 A CN 201510797342A CN 105211599 A CN105211599 A CN 105211599A
- Authority
- CN
- China
- Prior art keywords
- chitosan oligosaccharide
- ruminant
- composite probiotics
- probiotics preparations
- cfu
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a kind of ruminant chitosan oligosaccharide composite probiotics preparations, described ruminant chitosan oligosaccharide compound probiotic comprises chitosan oligosaccharide, lactobacillus acidophilus, candida utili, Rhodopseudomonas palustris.The superior effect of ruminant chitosan oligosaccharide composite probiotics preparations of the present invention is: ruminant chitosan oligosaccharide composite probiotics preparations is a kind of pollution-free, noresidue, have no side effect, anti-disease, without resistance, somatotrophic additive, chitosan oligosaccharide compound probiotic biologically active agents can be widely used in its feeding industry, to the resistance against diseases improving ruminant, improve the price of deed, reduce feed cost, few with or without antibiotic, promote the sound development of livestock and poultry breeding industry, improve the quality of animal product, preserve the ecological environment and human health will bring obvious Social and economic benef@.
Description
Technical field
The invention belongs to the technical field of fermented feed containing probiotics, be specifically related to a kind of ruminant chitosan oligosaccharide composite probiotics preparations.
Background technology
From the fifties in last century, in animal diets, add antibiotic significantly promote animal productiong, and major contribution has been made to the development of intensive animal husbandry.But As time goes on, the harm adding anti-element in feed manifests day by day, and be subject to the extensive concern of society.
European Union completely forbids and add antibiotic in animal and fowl fodder from January, 2006.Must not add antibiotic in livestock is raised has been internationally recognized food security standard.
Fermented feed containing probiotics technology grows up recently to have the novel fodder technology of many advantages, feed contains more active probiotic thalline, various enzyme, metabolite at different levels, multivitamin, protein breakdown products, active small peptide, amino acid, antibacterial substance, the Immune-enhancing effect factor, somatomedin etc. after probiotics fermention, play growth promoting effects, maintain the colony balance effect of animal intestinal; Owing to not adding antibiotic etc, can not cause antibiotics residues, fermented feed containing probiotics technology is a kind of environmental health type Feed Production Technology.
Probio can adhere on intestines parietal cell and play barrier action, and Reverse transcriptase repels pathogen.Multiple probio can suppress the Growth and Reproduction of harmful bacteria in the gastrointestinal tract, improves resistance against diseases.
In recent years research shows, no matter be in basic life process, as the maintenance aspect of fertilization, generation, growth, differentiation, nervous system, immune system steady state, or in the generation and development of disease, as all related to the participation of oligonucleotide chain in the process such as inflammation and pathogenic infection, oligonucleotide chain plays specific identification and mediation in these life and lysis.
Current research finds that oligosaccharides isoreactivity Small molecular is except regulating microecological balance as cultivated animals alimentary canal Bifidobacterium MF (bifidusfactor), also there is the secretion of induction IGF-I and GH, increase the gene expression of IGF-I, improve the synthesis capability of protein, accelerate the metabolism of lipid; Promote the IL-1 beta gene expression in lymphonodi mesenterici; Animal alimentary canal morphosis and function are obviously improved.
Research finds that current nature finds that uniquely positively charged oligosaccharides-chitosan oligosaccharide is different from other common oligosaccharides (as: FOS, wood oligose etc.) structure and action target spot, and its major function of chitosan oligosaccharide is to the adjustment that cellulous function is perhaps carried out after entering blood by duodenal absorption.And as: directly do not absorb as blood at duodenum FOS, wood oligose etc., mainly played a role by regulating intestinal canal profitable strain at large intestine.And chitosan oligosaccharide is except the common feature with oligosaccharides, also there are some special function and efficacies.
Add chitosan oligosaccharide in feed to be conducive to improving cultivated animals mucosal structure, improving digestive organs function, promote absorption and the cultivated animals growth of nutriment, improve rate of body weight gain and the feed conversion rate of cultivated animals.Chitosan oligosaccharide can with some endotoxins (as LPS), virus and eukaryotic surface conjunction, in conjunction with after chitosan oligosaccharide as the auxiliary agent of exogenous antigen or vaccine adjuvant, increase tiring of antigen or vaccine; Improve body antibody horizontal, promote the phagocytic activity of macrophage, improve body anti-infection ability; Improve vegetalitas lymphocyte (PHA lymphocyte) conversion ratio and the T-TLC (CD3) of animal; Promote the differentiation of B cell and the generation of cell factor, cell factor can promote lymphocytic propagation, differentiation, strengthens the resistance to infecting, and participates in the immune response and the inflammatory reaction that regulate body.But existing domestic green feed additive has pollution, residual and toxic and side effect.
Oligosaccharide feed additive is a kind of novel green feed additive, and development time is shorter, at the early-stage; High-speed development period is in for the research on its basis and research and development of products; Current oligosaccharide feed additive is progressively familiar with by people, and its feeding effect is also also just accepted by accreditation; China can produce some oligosaccharide feed additive products, as manna oligosacchride, wood oligose, FOS, soy oligosaccharide etc.Chitosan oligosaccharide is listed in by Ministry of Agriculture's latest edition (2012) feed addictive catalogue.
Summary of the invention
The invention provides a kind of ruminant chitosan oligosaccharide composite probiotics preparations, to solve the technical problem that existing domestic green feed additive has pollution, residual and toxic and side effect.
In order to solve above technical problem, the technical scheme that the present invention takes is:
A kind of ruminant chitosan oligosaccharide composite probiotics preparations, it is characterized in that, described ruminant chitosan oligosaccharide composite probiotics preparations comprises chitosan oligosaccharide, lactobacillus acidophilus, candida utili, moisture and Rhodopseudomonas palustris.
Be preferably, described chitosan oligosaccharide is 0.1%-9.0%.
Be preferably, described lactobacillus acidophilus is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g.
Be preferably, described candida utili is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g.
Be preferably, described Rhodopseudomonas palustris is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g.
Be preferably, described moisture is 25%-50%.
A production technology for ruminant chitosan oligosaccharide compound probiotic, described production technology comprises the steps:
raw bacteria preparation→
bottling (tank) packaging→
quality inspection→
finished product is put in storage→
keep sample standby picking up
After employing technique scheme; ruminant chitosan oligosaccharide composite probiotics preparations of the present invention is a kind of pollution-free, noresidue, have no side effect, anti-disease, without resistance, somatotrophic additive; chitosan oligosaccharide compound probiotic biologically active agents can be widely used in its feeding industry; to the raising price of deed; few with or without antibiotic; reduce feed cost; promote the development of livestock and poultry breeding industry; the quality of raising animal product, preserves the ecological environment and human health will bring obvious Social and economic benef@.
Detailed description of the invention
Ruminant chitosan oligosaccharide composite probiotics preparations comprises chitosan oligosaccharide, lactobacillus acidophilus, candida utili, Rhodopseudomonas palustris and moisture.Chitosan oligosaccharide is 0.1%-9.0%, and lactobacillus acidophilus is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g, candida utili is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g, Rhodopseudomonas palustris is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g, moisture is 25%-50%.
A production technology for ruminant chitosan oligosaccharide compound probiotic, described production technology comprises the steps:
raw bacteria preparation→
bottling (tank) packaging→
quality inspection→
finished product is put in storage→
keep sample standby picking up
Ruminant chitosan oligosaccharide composite probiotics preparations needs to meet the requirement of health Testing index, and described sanitary index is:
Aspergillus flavus mould toxin B1 :≤10.0 μ g/kg;
Arsenic (in total arsenic) :≤2.0mg/kg;
Plumbous (in pb) :≤5.0mg/kg;
Fluorine (in F) :≤1000mg/kg;
Mercury (in Hg) :≤0.1mg/kg;
Cadmium (in cd) :≤0.5mg/kg;
Miscellaneous bacteria rate :≤0.5%;
Escherichia coli :≤1.0 × 10
5individual/kg:
Total number of molds :≤2.0 × 10
7individual/kg;
Salmonella: must not detect;
Pathogenic bacteria (pathogenic entero becteria and pathogenic bacteria property coccus): must not detect.
Wherein the chitosan oligosaccharide raw material of ruminant chitosan oligosaccharide compound probiotic adopts the chitosan oligosaccharide of Zhong Ke James Gleick biotech firm, adopt international advanced enzyme reaction-UF membrane coupled biological technology to carry out biology extraction to form, to be formed by connecting with glycosidic bond by 2-10 monose and the minuent with straight or branched is polymerized carbohydrate, to guarantee that ruminant can reach the effect in feed applications after nursing;
(1) chitosan oligosaccharide can improve cultivated animals mucosal structure, improve digestive organs function, promotes absorption and the cultivated animals growth of nutriment, improves rate of body weight gain and the feed conversion rate of cultivated animals.
(2) chitosan oligosaccharide can with some endotoxins (as LPS), virus and eukaryotic surface conjunction, in conjunction with after chitosan oligosaccharide as the auxiliary agent of exogenous antigen or vaccine adjuvant, increase tiring of antigen or vaccine.
(3) improve body antibody horizontal, promote the phagocytic activity of macrophage, improve body anti-infection ability.
(4) vegetalitas lymphocyte (PHA lymphocyte) conversion ratio and the T-TLC (CD3) of animal is improved.
(5) promote the differentiation of B cell and the generation of cell factor, cell factor can promote lymphocytic propagation, differentiation, strengthens the resistance to infecting, and participates in the immune response and the inflammatory reaction that regulate body.
Ruminant chitosan oligosaccharide composite probiotics preparations of the present invention is a kind of pollution-free, noresidue, have no side effect, anti-disease, without resistance, somatotrophic additive; chitosan oligosaccharide compound probiotic biologically active agents can be widely used in its feeding industry; to the raising price of deed; few with or without antibiotic; reduce feed cost; promote the development of livestock and poultry breeding industry, the quality of raising animal product, preserves the ecological environment and human health will bring obvious Social and economic benef@.
Last it is noted that above embodiment only illustrates technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (6)
1. a ruminant chitosan oligosaccharide composite probiotics preparations, is characterized in that, described ruminant chitosan oligosaccharide composite probiotics preparations comprises chitosan oligosaccharide, lactobacillus acidophilus, candida utili, moisture and Rhodopseudomonas palustris.
2. ruminant chitosan oligosaccharide composite probiotics preparations according to claim 1, is characterized in that, described chitosan oligosaccharide is 0.1%-9.0%.
3. ruminant chitosan oligosaccharide composite probiotics preparations according to claim 1, is characterized in that, described lactobacillus acidophilus is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g.
4. ruminant chitosan oligosaccharide composite probiotics preparations according to claim 1, is characterized in that, described candida utili is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g.
5. ruminant chitosan oligosaccharide composite probiotics preparations according to claim 1, is characterized in that, described Rhodopseudomonas palustris is 1.00 × 10
2cfu/g-1.00 × 10
12cfu/g.
6. ruminant chitosan oligosaccharide composite probiotics preparations according to claim 1, is characterized in that, described moisture is 25%-50%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510797342.5A CN105211599A (en) | 2015-11-18 | 2015-11-18 | A kind of ruminant chitosan oligosaccharide composite probiotics preparations |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510797342.5A CN105211599A (en) | 2015-11-18 | 2015-11-18 | A kind of ruminant chitosan oligosaccharide composite probiotics preparations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105211599A true CN105211599A (en) | 2016-01-06 |
Family
ID=54981215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510797342.5A Pending CN105211599A (en) | 2015-11-18 | 2015-11-18 | A kind of ruminant chitosan oligosaccharide composite probiotics preparations |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105211599A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919494A (en) * | 2010-09-30 | 2010-12-22 | 大连三科生物工程有限公司 | Complex microorganism additive as well as preparation method and application thereof |
| CN102423015A (en) * | 2011-11-29 | 2012-04-25 | 天津中裕维特生物科技有限公司 | Special synbioticmbiotic for broiler |
| CN104171363A (en) * | 2014-07-02 | 2014-12-03 | 中泰和（北京）科技发展有限公司 | Feed additive for reducing diarrhea rate of piglets |
| CN104397353A (en) * | 2014-12-03 | 2015-03-11 | 青岛根源生物技术集团有限公司 | Probiotics for preventing diarrhea of livestock and poultry and application thereof |
| CN104824350A (en) * | 2015-04-15 | 2015-08-12 | 浙江绿发饲料科技有限公司 | Drug-free growing-fattening pig feed |
-
2015
- 2015-11-18 CN CN201510797342.5A patent/CN105211599A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919494A (en) * | 2010-09-30 | 2010-12-22 | 大连三科生物工程有限公司 | Complex microorganism additive as well as preparation method and application thereof |
| CN102423015A (en) * | 2011-11-29 | 2012-04-25 | 天津中裕维特生物科技有限公司 | Special synbioticmbiotic for broiler |
| CN104171363A (en) * | 2014-07-02 | 2014-12-03 | 中泰和（北京）科技发展有限公司 | Feed additive for reducing diarrhea rate of piglets |
| CN104397353A (en) * | 2014-12-03 | 2015-03-11 | 青岛根源生物技术集团有限公司 | Probiotics for preventing diarrhea of livestock and poultry and application thereof |
| CN104824350A (en) * | 2015-04-15 | 2015-08-12 | 浙江绿发饲料科技有限公司 | Drug-free growing-fattening pig feed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ASML A et al. | The beneficial role of probiotics in monogastric animal nutrition and health | |
| CN104195075B (en) | Enterococcus faecium EF08 as well as feed additive and feed containing enterococcus faecium EF08 | |
| US8496923B2 (en) | Probiotic treatment for infectious gastrointestinal diseases | |
| US20060008511A1 (en) | Probiotic products for pet applications | |
| AU2009293213B2 (en) | Method for using a Bacillus subtilis strain to enhance animal health | |
| CN102356828B (en) | Piglet early-stage microbial fermented antibiotic-free feed | |
| CN101366734A (en) | Synbiotics medicament composition | |
| CN102524539B (en) | Animal health preserver composition and preparation process for animal health preserver composition | |
| Radzikowski | Effect of probiotics, prebiotics and synbiotics on the productivity and health of dairy cows and calves | |
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| JP2008013534A (en) | Probiotics product for pet animal | |
| JP3648233B2 (en) | Feed composition | |
| CN103289910A (en) | Solid fermentation production method of bacillus coagulans | |
| CN104171429A (en) | Use of lactobacillus plantarum LP28 | |
| CN110326708A (en) | A kind of functionality additive package and its application | |
| CN110692847A (en) | Antibiotic-free mixed feed additive for rabbits | |
| JP5499231B2 (en) | Animal feed composition containing Lactobacillus plantarum, combined animal feed containing the composition, and method for maintaining or growing the Lactobacillus plantarum in the animal intestine | |
| KR100857771B1 (en) | Compositions for addition to feed for fish comprising Bacillus polyfermenticus, Bacillus licheniformis and Saccharomyces serevisiae | |
| WO2005087241A1 (en) | Infection-controlling agent for livestock, poultry or fish | |
| Hamasalim | The impact of some widely probiotic (Iraqi probiotic) on health and performance | |
| CN107647090A (en) | Efficient preventing piglet diarrhea probiotics | |
| CN107189966A (en) | A kind of probiotics and preparation method thereof | |
| CN103815143A (en) | Livestock feed additive capable of improving nonspecific immunity of livestock | |
| JP7092785B2 (en) | Feed additive preparation, its manufacturing method and usage method | |
| CN102754733A (en) | Novel micro-ecologic preparation for pig and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160106 |