CN104171363A - Feed additive for reducing diarrhea rate of piglets - Google Patents
Feed additive for reducing diarrhea rate of piglets Download PDFInfo
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- CN104171363A CN104171363A CN201410312338.0A CN201410312338A CN104171363A CN 104171363 A CN104171363 A CN 104171363A CN 201410312338 A CN201410312338 A CN 201410312338A CN 104171363 A CN104171363 A CN 104171363A
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- feed additive
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- chitosan oligosaccharide
- lactobacillus
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- 206010012735 Diarrhoea Diseases 0.000 title claims abstract description 32
- 239000003674 animal food additive Substances 0.000 title claims abstract description 13
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 claims abstract description 22
- 230000000529 probiotic effect Effects 0.000 claims abstract description 18
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 17
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 17
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 17
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 17
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 17
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims abstract description 17
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 17
- 239000006041 probiotic Substances 0.000 claims abstract description 17
- 235000018291 probiotics Nutrition 0.000 claims abstract description 17
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 10
- 241001465754 Metazoa Species 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 6
- 231100000331 toxic Toxicity 0.000 abstract description 3
- 230000002588 toxic effect Effects 0.000 abstract description 3
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 244000144972 livestock Species 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 19
- 241000282898 Sus scrofa Species 0.000 description 4
- 235000014590 basal diet Nutrition 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 241000282887 Suidae Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000007726 management method Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 231100000957 no side effect Toxicity 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000002302 glucosamines Chemical class 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000005453 pelletization Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Landscapes
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a feed additive for reducing diarrhea rate of piglets, belongs to the technical field of animal feeds and is applied to the livestock breeding industry. The green feed additive for reducing the diarrhea rate of piglets is composed of chitosan oligosaccharide and a complex probiotic, wherein the mass ratio of the chitosan oligosaccharide to the complex probiotic is in a range of 1:5 to 1:20. The complex probiotic is a composition which is prepared from bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis. According to the invention, the feed additive contains no heavy metal and is low in required adding amount. The feed additive is environment-friendly since no toxic and side effect is caused to the piglets and no residue is left after use. The feed additive can reduce the diarrhea rate of the piglets and can significantly promote piglet growth at the same time.
Description
Technical field
The present invention relates to a kind of feed addictive that improves grice diarrhoea rate, belong to technical field of animal, be applied to livestock-raising industry.
Background technology
In current Swine Production, piglet diarrhoea is comparatively general.Piglet is caused particularity and some defects of its digestive physiology system because of its intestines and stomach structure and physiogeny imperfection.Piglet growth speed is fast, metabolism is vigorous, strong to the demand of nutriment, but regulative mechanism imperfection, to various stress resistance ability, so piglet is easily suffered from diarrhoea.In actual production, grice diarrhoea rate is generally 20% one 30%, the death rate 2% one 4%.Aspect nutrition, conventionally adopt at present a large amount of zinc oxide that adds in feed to control grice diarrhoea, but the high zinc daily ration of Long-term Feeding, can suppress the growth of piglet on the one hand, reduce daily gain, the zinc excreting through ight soil in a large number has on the other hand caused serious pollution to environment.
Chitosan oligosaccharide is the product that shitosan further decomposes, and by 2~10 Glucosamines, by β-Isosorbide-5-Nitrae glycosidic bond, is formed by connecting, unique alkalescence, the positively charged animal fiber oligosaccharides of being that is that current occurring in nature is found.It is not decomposed in intestines and stomach, can be absorbed directly into blood by animal and act on target cell, continual and steady raising animal body immunity.Chitosan oligosaccharide can regulate peculiar information the function of target cell with chemical transfer mode, play a part courier, after information is transmitted, is excluded external, safe, nontoxic, noresidue.
Bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, lactobacillus lactis all belong to probio.Probio is the active microorganism culture that participates in microbial balance in animal intestine, can directly strengthen the inhibitory action of animal to intestinal toxic microbiologic population, also can carry out prevent disease by strengthening non-specific immune function, and indirectly play and promote growth of animal, improve food conversion ratio, improve the effects such as environment.Wherein, the main feature of bacillus preparation is resistance to extruding in high temperature resistant (100 ℃), acidproof, salt tolerant and pelletization, has good stability; Produce digestive ferment, there is protease, amylase and lipase active; In intestinal environment, do not breed, after enteron aisle field planting, can consume a large amount of oxygen, maintain enteron aisle anaerobic environment.And the metabolite of Bacillus acidi lactici can suppress the growth of Escherichia coli and salmonella.
Probiotic action works in enteron aisle, improve the diarrhoea of piglet, and chitosan oligosaccharide can be absorbed into blood by the balance of microorganism species in regulating intestinal canal, with the effect of signaling molecule, directly acts on immunocyte, improves the premunition of piglet.So the two compatibility has good synergy for control grice diarrhoea.
Summary of the invention
The object of this invention is to provide a kind of noresidue, have no side effect and improve safely and effectively the feed addictive of grice diarrhoea.
The present invention is achieved through the following technical solutions:
Improve a green feed additive for grice diarrhoea, chitosan oligosaccharide and compound probiotic, consist of, its mass ratio is 1:5-1:20;
Wherein, described compound probiotic is the composition of bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis;
The mass ratio of described bacillus subtilis, described bacillus licheniformis, described lactobacillus acidophilus and described lactobacillus lactis is 2:2:1:1;
The viable bacteria content of described each component is respectively: the viable bacteria content of bacillus subtilis >=6,000,000,000/g; Bacillus licheniformis >=6,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g; Lactobacillus acidophilus >=4,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g.
Described chitosan oligosaccharide and described compound probiotic mass ratio are preferably 1:5-1:15.
Described chitosan oligosaccharide and described compound probiotic mass ratio be 1:10 more preferably.
Beneficial effect of the present invention is:
1, the present invention adopts the chitosan oligosaccharide that can be absorbed by animal body and the probio compatibility only working at enteron aisle, by two kinds of approach, improve the immunity of piglet, make the effect of chitosan oligosaccharide and probio collaborative mutually, strengthen action effect separately, can more effectively improve the common diarrhoeal diseases of piglet.
2, feed addictive used in the present invention does not contain heavy metal, and additive capacity is low, and piglet is had no side effect, noresidue after using, be the feed addictive of environmental type, when improving grice diarrhoea rate, can significantly promote the growth of piglet, and environmentally safe.
The specific embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is described in further detail.But example is not as a limitation of the invention.
Embodiment 1
Improve a green feed additive for grice diarrhoea, chitosan oligosaccharide and compound probiotic, consist of, its mass ratio is 1:10.Wherein, described compound probiotic is the composition of bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis.The mass ratio of bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis is 2:2:1:1.
The viable bacteria content of described each component is respectively: the viable bacteria content of bacillus subtilis >=6,000,000,000/g; Bacillus licheniformis >=6,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g; Lactobacillus acidophilus >=4,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g.
This feed addictive is added in daily ration according to 0.03% of daily ration weight, carry out weanling pig test.
Select the 21 healthy DLY piglets anosis, that initial weight is basically identical in age in days left and right to carry out feeding experiment, test is divided into 2 groups, i.e. control group and test group.Control group fed basal diet, test group is added the feed addictive that mass ratio is 0.03% the present embodiment in basal diet.Every group of 3 repetitions, 8 pigs of every repetition, free choice feeding and freely drinking water.35 days experimental periods.Result of the test is in Table 1.
Each group diarrhoea number of table 1
? | A diarrhoea number | Diarrhoea ratio |
Control group | 8 | 33.33% |
Test group | 2 | 8.33% |
Result of the test shows, under same raising condition, the diarrhea rate of test group is starkly lower than control group.
Embodiment 2
Improve a green feed additive for grice diarrhoea, chitosan oligosaccharide and compound probiotic, consist of, its mass ratio is 1:20.Wherein, described compound probiotic is the composition of bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis.The mass ratio of bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis is 2:2:1:1.
The viable bacteria content of described each component is respectively: the viable bacteria content of bacillus subtilis >=6,000,000,000/g; Bacillus licheniformis >=6,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g; Lactobacillus acidophilus >=4,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g.
This feed addictive is added in feed according to 0.03% of daily ration weight, carry out weanling pig test.
Select 200 of the assorted 24 age in days weanling pigs of DLY ternary, be divided at random two groups, be i.e. control group and test group, every group of each 100 piglets.Control group fed basal diet, test group is added the feed addictive of 0.03% the present embodiment in basal diet.28 days experimental periods.Feeding and management is carried out according to the normal feeding and management method of plant.Result of the test is in Table 2.
The impact of each group of table 2 on grice diarrhoea and the incidence of disease
? | Diarrhoea (head) | Other morbidities (head) |
Control group | 14 | 16 |
Test group | 9 | 3 |
Result of the test shows, adds the incidence that compound formulation of the present invention can significantly reduce grice diarrhoea in feed, also can reduce the incidence of other diseases simultaneously.
Embodiment 3
Improve a green feed additive for grice diarrhoea, chitosan oligosaccharide and compound probiotic, consist of, its mass ratio is 1:5.Wherein, described compound probiotic is the composition of bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis.The mass ratio of bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis is 2:2:1:1.
The viable bacteria content of described each component is respectively: the viable bacteria content of bacillus subtilis >=6,000,000,000/g; Bacillus licheniformis >=6,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g; Lactobacillus acidophilus >=4,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g.
This feed addictive is added in feed according to 0.02% of daily ration weight, carry out weanling pig test.
220 of piglets selecting 21 ages in days wean, are divided into two groups, every group each 110 at random.
Control group is blank group, and test group is added the feed addictive of 0.03% the present embodiment on control group basis.Experimental period from wean start to wean the 65th day.Result of the test sees the following form.
Table 3 Production Performance of Weaning Pigs data
As shown in Table 3, compare with control group, the average daily gain of test group obviously improves, and diarrhea rate obviously declines.Wherein the diarrhea rate of test group declines 21.82% than control group.The incidence that compound formulation of the present invention can significantly reduce grice diarrhoea is added in explanation in feed.
Because constituent of the present invention is chitosan oligosaccharide and probio, in control grice diarrhoea, piglet itself is not had to toxic and side effect, in addition to environment also without any pollution, so, if this achievement is applied on a large scale, significant to the green cultivation of China, Green Meat industry development.The social benefit of this achievement and ecological benefits are significant.
Above-mentioned explanation to the disclosed embodiments, makes professional and technical personnel in the field can realize or use the present invention.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (3)
1. a green feed additive that improves grice diarrhoea, is characterized in that, chitosan oligosaccharide and compound probiotic, consists of, and its mass ratio is 1:5-1:20;
Wherein, described compound probiotic is the composition of bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus lactis;
The mass ratio of described bacillus subtilis, described bacillus licheniformis, described lactobacillus acidophilus and described lactobacillus lactis is 2:2:1:1;
The viable bacteria content of described each component is respectively: the viable bacteria content of bacillus subtilis >=6,000,000,000/g; Bacillus licheniformis >=6,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g; Lactobacillus acidophilus >=4,000,000,000/g; Lactobacillus lactis >=5,000,000,000/g.
2. a kind of green feed additive that improves grice diarrhoea according to claim 1, is characterized in that, described chitosan oligosaccharide and described compound probiotic mass ratio are 1:5-1:15.
3. a kind of green feed additive that improves grice diarrhoea according to claim 2, is characterized in that, described chitosan oligosaccharide and described compound probiotic mass ratio are 1:10.
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Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104605165A (en) * | 2015-03-02 | 2015-05-13 | 武汉新华扬生物股份有限公司 | Compound feed additive replacing feed antibiotics in piglet daily diet, and applications of compound feed additive |
CN104758318A (en) * | 2015-03-06 | 2015-07-08 | 山东宝来利来生物工程股份有限公司 | A composite preparation for reducing a diarrhea rate of weaned piglets and a preparing method thereof |
CN105053514A (en) * | 2015-07-24 | 2015-11-18 | 河南仰韶生化工程有限公司 | Compound microbial feed additive and production method thereof |
CN105211599A (en) * | 2015-11-18 | 2016-01-06 | 乌鲁木齐格莱克工程技术有限公司 | A kind of ruminant chitosan oligosaccharide composite probiotics preparations |
CN105285399A (en) * | 2015-11-16 | 2016-02-03 | 通威股份有限公司 | Liquid state composite microecological preparation for pigling and preparation method thereof |
CN106071233A (en) * | 2016-06-21 | 2016-11-09 | 湖北省农业科学院畜牧兽医研究所 | A kind of feed additive and method being applicable to produce drug residue free high quality pork |
CN106721068A (en) * | 2016-12-26 | 2017-05-31 | 成都合生元生物科技有限公司 | Composition and feed for adjusting animal intestinal tract microbial environment |
CN107115365A (en) * | 2017-05-09 | 2017-09-01 | 黑龙江省兽医科学研究所 | One kind preventing and treating diarrhea of pigs biological agent and preparation method thereof |
CN109329566A (en) * | 2018-12-05 | 2019-02-15 | 湖南赛福资源饲料科技有限公司 | For improving the premixed feed and preparation method of piglet gastrointestinal health |
CN111165676A (en) * | 2020-01-13 | 2020-05-19 | 青岛博隆实验动物有限公司 | Additive for colostrum substitute of puppy, preparation method and application |
CN111436529A (en) * | 2020-04-26 | 2020-07-24 | 重庆市畜牧科学院 | Compound feed for improving intestinal health of weaned piglets |
CN111513204A (en) * | 2016-11-10 | 2020-08-11 | 南京工业大学 | Application of feed additive in promoting intestinal development of weaned piglets |
CN112266880A (en) * | 2020-10-20 | 2021-01-26 | 青岛普罗百世生物科技有限公司 | Lactobacillus acidophilus and application thereof in weaned pig feed |
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Cited By (14)
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CN104605165A (en) * | 2015-03-02 | 2015-05-13 | 武汉新华扬生物股份有限公司 | Compound feed additive replacing feed antibiotics in piglet daily diet, and applications of compound feed additive |
CN104758318A (en) * | 2015-03-06 | 2015-07-08 | 山东宝来利来生物工程股份有限公司 | A composite preparation for reducing a diarrhea rate of weaned piglets and a preparing method thereof |
CN105053514A (en) * | 2015-07-24 | 2015-11-18 | 河南仰韶生化工程有限公司 | Compound microbial feed additive and production method thereof |
CN105285399A (en) * | 2015-11-16 | 2016-02-03 | 通威股份有限公司 | Liquid state composite microecological preparation for pigling and preparation method thereof |
CN105211599A (en) * | 2015-11-18 | 2016-01-06 | 乌鲁木齐格莱克工程技术有限公司 | A kind of ruminant chitosan oligosaccharide composite probiotics preparations |
CN106071233A (en) * | 2016-06-21 | 2016-11-09 | 湖北省农业科学院畜牧兽医研究所 | A kind of feed additive and method being applicable to produce drug residue free high quality pork |
CN111513204A (en) * | 2016-11-10 | 2020-08-11 | 南京工业大学 | Application of feed additive in promoting intestinal development of weaned piglets |
CN111513204B (en) * | 2016-11-10 | 2023-07-18 | 南京工业大学 | Application of feed additive in promoting intestinal development of weaned piglets |
CN106721068A (en) * | 2016-12-26 | 2017-05-31 | 成都合生元生物科技有限公司 | Composition and feed for adjusting animal intestinal tract microbial environment |
CN107115365A (en) * | 2017-05-09 | 2017-09-01 | 黑龙江省兽医科学研究所 | One kind preventing and treating diarrhea of pigs biological agent and preparation method thereof |
CN109329566A (en) * | 2018-12-05 | 2019-02-15 | 湖南赛福资源饲料科技有限公司 | For improving the premixed feed and preparation method of piglet gastrointestinal health |
CN111165676A (en) * | 2020-01-13 | 2020-05-19 | 青岛博隆实验动物有限公司 | Additive for colostrum substitute of puppy, preparation method and application |
CN111436529A (en) * | 2020-04-26 | 2020-07-24 | 重庆市畜牧科学院 | Compound feed for improving intestinal health of weaned piglets |
CN112266880A (en) * | 2020-10-20 | 2021-01-26 | 青岛普罗百世生物科技有限公司 | Lactobacillus acidophilus and application thereof in weaned pig feed |
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