CN101906389B - Micro-ecological preparation for promoting cyclic utilization of substrate sludge nitrogen and phosphorus and preparation method and application thereof - Google Patents
Micro-ecological preparation for promoting cyclic utilization of substrate sludge nitrogen and phosphorus and preparation method and application thereof Download PDFInfo
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- CN101906389B CN101906389B CN2009101507718A CN200910150771A CN101906389B CN 101906389 B CN101906389 B CN 101906389B CN 2009101507718 A CN2009101507718 A CN 2009101507718A CN 200910150771 A CN200910150771 A CN 200910150771A CN 101906389 B CN101906389 B CN 101906389B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a micro-ecological preparation for promoting circulation and release of substrate sludge nitrogen and phosphorus, which adopts a solid in situ mixed fermentation mode to culture a microbe capable of promoting the circulation (release) of the substrate sludge nitrogen and phosphorus, wherein the used fermentation substrate comprises the following components in part by weight: 30 to 40 parts of bran, 60 to 70 parts of fungus chaff, and 0.5 to 2 parts of inorganic salts. Then 5 to 15 parts of the micro-ecological preparation, 40 to 80 parts of kaolin, 5 to 10 parts of potassium permanganate, 5 to 10 parts of iron trichloride and 10 to 20 parts of calcium peroxide are mixed together for granulation to obtain a granular preparation which is used for promoting the circulation of the substrate sludge nitrogen and phosphorus and the degradation of substrate sludge organic matters. By using the micro-ecological preparation, on the one hand, a good ecological environment for the growth of aquatic livestock is created to fulfill the aim of aquatic product healthy breeding; and on the other hand, the input of fertilizer is reduced to achieve the full utilization of culture resources and reduce the production use cost.
Description
Technical field
The present invention relates to a kind of microbial preparation, relate in particular to a kind of probiotics that can promote the recycle of substrate sludge nitrogen phosphorus and preparation method thereof, also relate to the application of this microbial preparation in aquaculture.
Background technology
Current aquaculture high-density is put in a suitable place to breed, and high feeding rate causes the aquaculture water sh; Culture the organic excessively accumulation of bed mud, nitrogenous, phosphorus substance deposits in a large number, and objectionable impurities accumulates in a large number; Pathogenic micro-organism and parasite grow in a large number; Bring great hidden danger and threat for the health of culturing object, cause breeding environment badly to increasingly sharpen, disease frequently takes place.Wherein nitrogenous substances is mainly derived from feed and ight soil; Phosphorus containg substances is mainly derived from feed and fertilizer (like superphosphate of lime etc.), reaches as high as more than 90%.Correlative study is the result show, the bait of throwing in the feeding process, fertilizer account for 49.7%～54.5% and 47.5%～50.1% of the total input of nitrogen respectively, accounts for 30.0%～34.7% and 65.1%～69.9% of the total input of phosphorus.And the bed mud deposition is the principal mode of nitrogen in the pond culture system, phosphorus output; The ratio of its work output in general output accounted for more than 50%; It is thus clear that; Bed mud is that great majority add the nitrogen in the aquaculture pond, the final admittance person of phosphorus to, and the nitrogen of high loading, phosphorus also become the available valuable source of aquaculture in the bed mud.Bed mud also is a pollution sink, and high-pollution load must grow various pathogenic bacterias and parasite, becomes the sick pest bed of explosive fishes and shrimps.The reparation of the bed mud ecosystem and how to accelerate the circulation of bed mud available nitrogen, phosphorus and discharge become that healthy ecology is the most popular in culturing, one of the research topic of most critical.
The focus of correlative study at present all is that the mode that concentrates on chemistry discharges the deposition nitrogen phosphorus in the bed mud, yet the main drawback of chemical mode is the cost height, and the time length is short, and release efficiency is low, does not have actual application value basically.Through replenishing indigenous high-activity microorganism crowd, repair bed mud top layer micro-ecological environment, liberating nitrogen phosphorus to reach, improve the bed mud microenvironment, thereby the research that improves aquaculture water primary productivity, increase planktonic organism amount aspect does not appear in the newspapers.
Summary of the invention
The object of the present invention is to provide a kind of substrate sludge nitrogen, phosphorus circulation and probiotics that discharges of promoting; Utilizing nitrogen with difficulty in the conversion bed mud is available nitrogen; Insoluble phosphorus is a titanium pigment; With the effective recycle (promptly transforming and release) that realizes the nitrogen phosphoric, create the good ecotope of aquatic animal to grow on the one hand, reach the purpose of aquatic products healthy aquaculture; Reduce the input of fertilizer on the other hand, reach full utilization of culture resources, reduce the production use cost.
For achieving the above object, the present invention adopts following technical scheme:
A kind of probiotics that promotes the recycle of substrate sludge nitrogen phosphorus is prepared by following method:
Adopt solid in situ mixed fermentation mode can promote the mikrobe of substrate sludge nitrogen phosphorus recycle, the ratio of used fermented substrate is: wheat bran 30-40 part, bacterium chaff 60-70 part, inorganic salt 0.5-2 part.Adopt wheat bran and these two kinds of fermented products of bacterium chaff to be because its cost is low; And obtain easily, and by the fermentation of carbon nitrogen source nutrition-allocated proportion after this mixed and the suitable target microorganism of matrix sedimentation, especially preferred proportioning is 30 parts in a wheat bran; 70 parts of bacterium chaffs, 1.5 parts in inorganic salt.
Wherein, said inorganic salt are sodium-chlor, calcium chloride etc.
Obtain promoting efficiently the microbial strains of substrate sludge nitrogen phosphorus efficiency recycle through screening, what promote that nitrogen discharges be Bacillus licheniformis, and promotion phosphorus round-robin is a bacillus megaterium.
Through Study on Fermentation, obtain promoting the high density mixed combined fermentation technology of mikrobe of substrate sludge nitrogen phosphorus recycle, its fermentation condition is that postvaccinal matrix is fermented controlled temperature 28-35 ℃ flexible sealing in the pore mesh bag.
Preferably, probiotics of the present invention obtains utilizing storing through granulating process, uses the granule that is easier to bring into play effect conveniently and after using.Said granule comprises the composition of following components by weight percent:
Said probiotics 5-15 part, kaolin 40-80 part, potassium permanganate 5-10 part, iron trichloride 5-10 part, calcium superoxide 10-20 part.10 parts of preferred probioticses, 50 parts of kaolin, 10 parts in potassium permanganate, 10 parts of iron trichlorides, 20 parts of calcium superoxides.
The preparation method of said granule is: take by weighing behind each raw material and the said probiotics mixing granulation that ferments according to said ratio, be used to promote circulation of substrate sludge nitrogen phosphorus and bed mud organic matter degradation.Wherein said mixing granulation is to adopt prior art, with the extrusion bonded back cutting of moisture 18～20% matrix granulating.
The present invention also provides the application method of said microbial preparation in aquaculture, is that every mu of each throwing is executed microbial preparation 50 grams according to the invention, and use 30-40 the whole year, uses the 1.5-2.0 kilogram.
The present invention is directed to rich water and quality problem in the present aquaculture process; Introduce first and discharge substrate sludge nitrogen phosphorus with the viewpoint of instead of part chemical fertilizer with minimizing fertilizer application frequency and quantity; Screening obtains promoting the probiotics of substrate sludge nitrogen phosphorus circulation (release), realizes innoxious, the resource utilization of bed mud; Modern original position solid mixed fermentation technology is adopted in probiotics production of the present invention, and effectively viable bacteria content embodies very high creativeness and pioneering up to 1,000 hundred million/gram, and production cost is reduced greatly.Below be that microbial preparation of the present invention is prepared into behind the granule product comparison with prior art products:
| Project | Similar technology and product | Technology of the present invention and product |
| Formulation | Pulvis | Granule |
| Main raw material(s) | Industrial chemicals | Farm crop tangerine bar and small amounts of inorganic salt |
| The mode of production | Chemosynthesis | The solid culture crushed after being dried is added auxiliary material and is processed granule. |
| Production cost | High | Low |
| Product function | Through dissolving bed mud deposition nitrogen, phosphorus, replenish water nutrition or control bed mud fermentation using bacteria, alleviate water quality deterioration. | Through promoting substrate sludge nitrogen phosphorus round-robin microbiological degradation to discharge substrate sludge nitrogen phosphorus nutrient.The prevention water quality deterioration from the source. |
| Quality | The Chemicals standard | Effective viable bacteria content 10,000,000,000/gram; Stable, the gemma rate is more than 90% |
| Use cost | 10-15 unit/mu | 4-6 unit/mu |
| Effect | Generally | Good |
Use this complex microorganism preparations, can make the available nitrogen burst size account for the 20-30% of bed mud total nitrogen, the available phosphorus burst size accounts for bed mud total phosphorus 30-50%.
Description of drawings
Fig. 1 is the preparation flow figure of probiotics of the present invention.
Embodiment
Below in conjunction with embodiment the present invention is described further, it should be understood that these embodiment only are used for the purpose of illustration, never limit protection scope of the present invention.Those skilled in the art is understood, and in spirit that claim of the present invention limited and scope, can carry out many changes to it, revise, in addition equivalent, but all will fall in protection scope of the present invention.
The preparation of embodiment 1 probiotics
Wheat bran is skin remaining behind the wheat processing flour of directly buying; The bacterium chaff is pulverized and is got for cultivating matrix behind the edible mushrooms; Bacillus licheniformis and bacillus megaterium are purchased in Chinese industrial microbial strains preservation administrative center; The Bacillus licheniformis numbering: CICC 20227, and the bacillus megaterium numbering: CICC 20611.
Adopt the mode of solid in situ mixed fermentation:
The ratio of used fermented substrate is: 30 parts in wheat bran, 70 parts of bacterium chaffs, 1.5 parts in sodium-chlor; Earlier with fermented substrate and inorganic salt thorough mixing; 115 ℃ of following moist heat sterilizations 30 minutes; Bacillus licheniformis and bacillus megaterium are inoculated by every kilogram of matrix 10-20ml bacterium liquid in the cooling back, the girth 35cm that packs into behind the thorough mixing, and flexible the sealing in the pore mesh bag of long 40cm fermented; Controlled temperature 28-35 ℃, fermented 48 hours.
Embodiment 2
The material therefor source is with embodiment 1.The ratio of used fermented substrate is: 40 parts in wheat bran, 70 parts of bacterium chaffs, 2 parts in calcium chloride; Earlier with fermented substrate and inorganic salt thorough mixing; 115 ℃ of following moist heat sterilizations 30 minutes; Bacillus licheniformis and bacillus megaterium are inoculated by every kilogram of matrix 10-20ml bacterium liquid in the cooling back, the girth 35cm that packs into behind the thorough mixing, and flexible the sealing in the pore mesh bag of long 40cm fermented; Controlled temperature 28-35 ℃, fermented 48 hours.
Embodiment 3
The material therefor source is with embodiment 1.The ratio of used fermented substrate is: 40 parts in wheat bran, 60 parts of bacterium chaffs, 0.5 part in sodium-chlor; Earlier with fermented substrate and inorganic salt thorough mixing; 115 ℃ of following moist heat sterilizations 30 minutes; Bacillus licheniformis and bacillus megaterium are inoculated by every kilogram of matrix 10-20ml bacterium liquid in the cooling back, the girth 35cm that packs into behind the thorough mixing, and flexible the sealing in the pore mesh bag of long 40cm fermented; Controlled temperature 28-35 ℃, fermented 48 hours.
The preparation of embodiment 4 granules
With 50 parts of kaolin, 10 parts in potassium permanganate, 10 parts of the probioticses that ferments in 10 parts of the iron trichlorides, 20 parts of calcium superoxides and embodiment 1 are with the extrusion bonded back cutting of moisture 19% matrix granulating.
Embodiment 5
With 40 parts of kaolin, 5 parts in potassium permanganate, 5 parts of the probioticses that ferments in 5 parts of the iron trichlorides, 10 parts of calcium superoxides and embodiment 2 are with the extrusion bonded back cutting of moisture 18% matrix granulating.
Embodiment 6
With 80 parts of kaolin, 10 parts in potassium permanganate, 15 parts of the probioticses that ferments in 10 parts of the iron trichlorides, 20 parts of calcium superoxides and embodiment 3 are with the extrusion bonded back cutting of moisture 20% matrix granulating.
Experimental example 1
This is tested, and Jiangxia District Bao Fu township plant of many Foucaults skill farm carried out in the Wuhan City in 2006 and the 5-10 month in 2007.Choose the breed pool of 2 parallel rankings, average area is 10 mu, and bed mud thickness is 8.5cm, and the depth of water keeps 1.5m, and aquaculture model is that raise together with in the pond, and stocking model is as shown in table 1.The 5Kg feed of throwing something and feeding every mu of every day, every mu of every month sky drops into 3 kilograms of monoammonium phosphates, 6 kilograms in urea.
Table 1 test pond mu stocking model
Experiment is provided with control group and experimental group.Bring into use the granule of embodiment 4 behind the experimental group mensuration initial value, added 1 time in per 10 days, last till that experiment finishes not change water in the experimentation, only additional evaporation and percolating water keep depth of water 1.5m.Equal bait throwing in every day of experimental session control group and test group 2 times, daily ration, feeding quantity is undertaken by the feed specified volume, and fertilising is by 7 days 1 time, and usage quantity is with reference to the fertilizer specification sheets.Total nitrogen, total phosphorus (seeing shown in table 2, the table 3) in the main mensuration of the experiment aquaculture water bed mud, method is with reference to the WQM standard.
Table 2 bed mud total nitrogen index unit: g/kg
Table 3 bed mud total phosphorus index unit: g/kg
When experiment finished, control group bed mud total nitrogen, total phosphorous had reached 1.080g/kg and 0.484g/kg, and experimental group is starkly lower than control group, significant difference.In experimental group; The nitrogen rate of release can reach 0.0016g/kg/d, and the phosphorus rate of release can reach 0.0011g/kg/d, by annual time of application 4-6 month; Can make the available nitrogen burst size account for the 20-30% of bed mud total nitrogen, the available phosphorus burst size accounts for bed mud total phosphorus 30-50%.
Claims (7)
1. probiotics that promotes the recycle of substrate sludge nitrogen phosphorus is prepared by following method:
Adopt solid in situ mixed fermentation mode can promote the mikrobe of substrate sludge nitrogen phosphorus recycle; The ratio of used fermented substrate is: wheat bran 30-40 part; Bacterium chaff 60-70 part, inorganic salt 0.5-2 part, that promote nitrogen release in the said mikrobe is Bacillus licheniformis CICC 20227; Promote that the phosphorus round-robin is bacillus megaterium CICC 20611, said inorganic salt are sodium-chlor or calcium chloride.
2. the probiotics of promotion substrate sludge nitrogen phosphorus according to claim 1 recycle, wherein, the ratio of used fermented substrate is: 30 parts in wheat bran, 70 parts of bacterium chaffs, 1.5 parts in inorganic salt.
3. the probiotics of promotion substrate sludge nitrogen phosphorus according to claim 1 recycle, wherein, the fermentation condition of said fermentation is that postvaccinal matrix is fermented controlled temperature 28-35 ℃ flexible sealing in the pore mesh bag.
4. probiotics granule that promotes the recycle of substrate sludge nitrogen phosphorus comprises the composition of following weight part:
The described probiotics 5-15 of claim 1 part, kaolin 40-80 part, potassium permanganate 5-10 part, iron trichloride 5-10 part, calcium superoxide 10-20 part.
5. the probiotics granule of promotion substrate sludge nitrogen phosphorus according to claim 4 recycle comprises the composition of following weight part:
10 parts of the described probioticses of claim 1,50 parts of kaolin, 10 parts in potassium permanganate, 10 parts of iron trichlorides, 20 parts of calcium superoxides.
6. the method for the probiotics granule of preparation claim 4 or 5 described promotion substrate sludge nitrogen phosphorus recycles is to adopt the prior art mixing granulation after taking by weighing each component by said proportioning.
7. the described probiotics of claim 1 is used for the application of aquaculture preparation in preparation.
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| CN2009101507718A CN101906389B (en) | 2009-06-30 | 2009-06-30 | Micro-ecological preparation for promoting cyclic utilization of substrate sludge nitrogen and phosphorus and preparation method and application thereof |
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| CN2009101507718A CN101906389B (en) | 2009-06-30 | 2009-06-30 | Micro-ecological preparation for promoting cyclic utilization of substrate sludge nitrogen and phosphorus and preparation method and application thereof |
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| CN101906389A CN101906389A (en) | 2010-12-08 |
| CN101906389B true CN101906389B (en) | 2012-07-04 |
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| CN104304142B (en) * | 2014-09-26 | 2017-03-29 | 浙江海洋学院 | A kind of environmental improvement method to Penaeus penicillatus (Alcock). nursery pond |
| CN104673728B (en) * | 2015-03-20 | 2019-01-11 | 济南航晨生物科技有限公司 | A kind of solid fermentation method in situ of agricultural gemma bacillus agent |
Citations (2)
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| CN101041807A (en) * | 2007-03-06 | 2007-09-26 | 北京万泰新源生物科技有限公司 | Fertilizer leaven and preparation method and application thereof |
| WO2009047406A3 (en) * | 2007-07-30 | 2009-06-18 | Degremont | Method and installation for biologically treating waste water |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041807A (en) * | 2007-03-06 | 2007-09-26 | 北京万泰新源生物科技有限公司 | Fertilizer leaven and preparation method and application thereof |
| WO2009047406A3 (en) * | 2007-07-30 | 2009-06-18 | Degremont | Method and installation for biologically treating waste water |
Non-Patent Citations (3)
| Title |
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| 卢显芝等.解磷芽孢杆菌及其对养殖池塘水体磷组分的转化研究.《黑龙江八一农垦大学学报》.2008,第20卷(第6期), * |
| 曹煜成等.地衣芽孢杆菌De在优质草鱼养殖中的应用研究.《南方水产》.2008,第4卷(第3期), * |
| 李谷等.养殖水体氮的生物转化及其相关微生物研究进展.《中国生态农业学报》.2006,第14卷(第1期), * |
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Owner name: WUHAN RICH GREEN AGRICULTRURAL BIOLOGY ENGINEERING Free format text: FORMER OWNER: WUHAN THRIVE BIOLOGICAL TECHNOLOGY CO., LTD. Effective date: 20150514 |
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Effective date of registration: 20150514 Address after: 430223 Hubei city of Wuhan province and Economic Development Zone Jiangxia road Hubei green green engineering development Limited by Share Ltd 1-10 comprehensive building Patentee after: WUHAN RICH GREEN AGRICULTURAL BIOLOGICAL ENGINEERING TECHNOLOGY CO., LTD. Address before: 430223, East Lake Wuhan hi tech Development Zone, Hubei Province Temple Mountain District green biological engineering Park Patentee before: Wuhan Thrive Biological Technology Co., Ltd. |
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