CN101857496B - Moth orchid culture medium - Google Patents
Moth orchid culture medium Download PDFInfo
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- CN101857496B CN101857496B CN201010190129.5A CN201010190129A CN101857496B CN 101857496 B CN101857496 B CN 101857496B CN 201010190129 A CN201010190129 A CN 201010190129A CN 101857496 B CN101857496 B CN 101857496B
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Abstract
The invention relates to a moth orchid culture medium, which mainly comprises macro compounds, trace compounds, organic elements, hormone and additives; the macro compounds mainly include anhydrous calcium chloride, ammonium nitrate and the like, wherein the concentration of each substance is 20mg/L to 1700mg/L; the trace compounds mainly include manganese sulfate, zinc sulfate and the like, wherein the concentration of each substance is 0.01mg/L to 20mg/L; the organic elements mainly include vitamin B1, nicotinic acid and the like, wherein the concentration of each substance is 0.05mg/L to 200mg/L; the hormone is 0.05mg/L to 1mg/L of naphthylacetic acid; the additives mainly include potato, banana and the like, wherein the concentration of each substance is 1g/L to 60g/L; and the rest is water. By regulating the varieties and added amount of the additives, the nutrition of the culture medium can become richer, the growth and rooting of the moth orchid can be greatly promoted, root bending and browning can be reduced, and thereby the quality of germchits is increased.
Description
Technical field the present invention relates to plant culture, particularly relates to a kind of moth orchid culture medium.
Background technology butterfly orchid is to cultivate in the world one of general cattleya kind widest in area, has the laudatory title of " cattleya queen ", and butterfly orchid type is large, and the florescence is long, beautiful in colour, with countless changes, is the ornamental cattleya kind of popularizing the most.The substratum of training about butterfly orchid group in the market has a variety of, and wherein composition and content are also not quite similar.But these substratum are unsatisfactory in the effect in butterfly orchid strengthening seedling and rooting stage, easily cause and stick up root, or root excessive growth and blade loses growth, the easy Necrosis of butterfly orchid seedling, butterfly orchid seedling quality is reduced greatly, be unfavorable for the market sale of butterfly orchid.
Summary of the invention is in order to overcome the deficiency of above-mentioned technical background, and the present invention increases some additives in formula, makes moth orchid culture medium comprehensive nutrition, can be good at promoting butterfly orchid strengthening seedling and rooting, improves the quality of bottle outlet seedling.
In order to reach the above object, the technical solution used in the present invention is:
A moth orchid culture medium, described substratum mainly comprises a large amount of compounds, minor compound class, organic element class, hormones, additive, wherein:
Described a large amount of compounds mainly comprises Calcium Chloride Powder Anhydrous, nitric acid ammonia, saltpetre, anhydrous magnesium sulfate, potassium primary phosphate, disodium ethylene diamine tetraacetate, ferrous sulfate, and wherein the concentration of each material in culture medium solution is 20～1700mg/L;
Described minor compound class mainly comprises manganous sulfate, zinc sulfate, copper sulfate, cobalt chloride, Sodium orthomolybdate, boric acid, potassiumiodide, and wherein the concentration of each material in culture medium solution is 0.01～20mg/L;
Described organic element class mainly comprises VITMAIN B1, nicotinic acid, vitamin B6, inositol, glycine, and wherein the concentration of each material in culture medium solution is 0.05～200mg/L;
Described hormones is naphthylacetic acid, and its concentration in culture medium solution is 0.05～1mg/L;
Affiliated additive mainly comprises potato, banana, white sugar, agar powder, gac, and wherein the concentration of each material in culture medium solution is 1～60g/L;
All the other are water.
Substratum main component described in the present invention is composed as follows in mg/L:
1 Calcium Chloride Powder Anhydrous 300-400
2 nitric acid ammonia 1500-1700
3 saltpetre 700-1000
4 anhydrous magnesium sulfate 80-100
5 potassium primary phosphate 70-100
6 disodium ethylene diamine tetraacetate 30-50
7 ferrous sulfate 20-40
8 manganous sulfate 10-20
9 zinc sulfate 3-6
10 copper sulfate 0.01-0.1
11 cobalt chloride 0.01-0.1
12 Sodium orthomolybdate 0.1-1.0
13 boric acid 2-5
14 potassiumiodide 0.1-1.0
15 VITMAIN B1 0.05-0.5
16 nicotinic acid 0.1-1.0
17 vitamin B6 0.1-1.0
18 inositol 50-200
19 glycine 1-5
20 naphthylacetic acid 0.05-1
21 potato 30000-60000
22 banana 30000-60000
23 white sugar 10000-30000
24 agar powders 48006000
25 gac 1000-3000
Preferably, substratum main component of the present invention is composed as follows in mg/L:
1 Calcium Chloride Powder Anhydrous 320-350
2 nitric acid ammonia 1600-1680
3 saltpetre 800-950
4 anhydrous magnesium sulfate 90-95
5 potassium primary phosphate 75-95
6 disodium ethylene diamine tetraacetate 30-40
7 ferrous sulfate 20-30
8 manganous sulfate 10-15
9 zinc sulfate 4-6
10 copper sulfate 0.01-0.05
11 cobalt chloride 0.01-0.05
12 Sodium orthomolybdate 0.1-0.5
13 boric acid 2-4
14 potassiumiodide 0.1-0.5
15 VITMAIN B1 0.05-0.3
16 nicotinic acid 0.1-0.8
17 vitamin B6 0.1-0.8
18 inositol 50-150
19 glycine 1-3
20 naphthylacetic acid 0.05-0.5
21 potato 40000-55000
22 banana 40000-55000
23 white sugar 15000-25000
24 agar powder 5000-5500
25 gac 1500-2500
Most preferred, medium component of the present invention is composed as follows in mg/L:
1 Calcium Chloride Powder Anhydrous 332
2 nitric acid ammonia 1650
3 saltpetre 950
4 anhydrous magnesium sulfates 90.5
5 potassium primary phosphates 85
6 disodium ethylene diamine tetraacetate 37.3
7 ferrous sulfate 27.8
8 manganous sulfates 11.15
9 zinc sulfate 4.3
10 copper sulfate 0.0125
11 cobalt chloride 0.0125
12 Sodium orthomolybdates 0.125
13 boric acid 3.1
14 potassiumiodides 0.42
15 VITMAIN B1 0.1
16 nicotinic acid 0.5
17 vitamin B6s 0.5
18 inositols 100
19 glycine 2
20 naphthylacetic acids 0.3
21 potatos 50000
22 bananas 50000
23 white sugar 20000
24 agar powders 5400
25 gacs 2000
Substratum of the present invention is to take water as solvent, and preferably deionized water, is mainly used in the strengthening seedling and rooting stage in the training of butterfly orchid group.When a large amount of compounds of preparation, weigh respectively and be dissolved in the water respectively; Minor compound class, organic element class and hormones must strictly be weighed with electronic balance, then are dissolved in water respectively, and then mix; In additive, potato will first be boiled and blend, and banana directly blends, and white sugar and agar powder need to add poach boiling until dissolve; Finally mix and be substratum of the present invention.
The present invention has increased additive in the formula of substratum, banana major function in additive is hestening rooting, but adds very fewly, and root system is flourishing not, adding too much and just easily cause and stick up root and excessive growth, is 3000～6000mg/L so will strictly control the add-on of banana; Potato can strong sprout, and can well improve substratum soft durometer, to sticking up root, also has certain weakening effect; The interpolation of gac can greatly reduce the generation of brown stain; Certain density naphthylacetic acid can promote the integral body growth of seedling.
The present invention, by regulating kind and the add-on of additive, makes substratum nutrition more comprehensively, can be good at promoting butterfly orchid strengthening seedling and rooting, reduces and sticks up root and brown stain, improves the quality of bottle outlet seedling.
The present invention is described further below in conjunction with specific embodiment for embodiment, the present invention discloses a kind of moth orchid culture medium, described substratum mainly comprises a large amount of compounds, minor compound class, organic element class, hormones and additive, wherein:
Described a large amount of compounds mainly comprises Calcium Chloride Powder Anhydrous, nitric acid ammonia, saltpetre, anhydrous magnesium sulfate, potassium primary phosphate, disodium ethylene diamine tetraacetate, ferrous sulfate, and wherein the concentration of each material is 20～1700mg/L;
Described minor compound class mainly comprises manganous sulfate, zinc sulfate, copper sulfate, cobalt chloride, Sodium orthomolybdate, boric acid, potassiumiodide, and wherein the concentration of each material in culture medium solution is 0.01～20mg/L;
Described organic element class mainly comprises VITMAIN B1, nicotinic acid, vitamin B6, inositol and glycine, and wherein the concentration of each material in culture medium solution is 0.05～200mg/L;
Described hormones is naphthylacetic acid, and its concentration in culture medium solution is 0.05～1mg/L;
Affiliated additive mainly comprises potato, banana, white sugar, agar powder or gac, and wherein the concentration of each material in culture medium solution is 1～60g/L;
All the other are water.
Embodiment 1
Preparation 10L butterfly orchid strengthening seedling and rooting substratum, take the following material in milligram: Calcium Chloride Powder Anhydrous 3320, nitric acid ammonia 16500, saltpetre 9500, anhydrous magnesium sulfate 905, potassium primary phosphate 850, manganous sulfate 111.5, zinc sulfate 43, copper sulfate 0.125, cobalt chloride 0.125, Sodium orthomolybdate 1.25, boric acid 31, potassiumiodide 4.2, disodium ethylene diamine tetraacetate 373, ferrous sulfate 278, VITMAIN B1 1, nicotinic acid 5, vitamin B6 5, inositol 1000, glycine 20, naphthylacetic acid 3, potato 500000, banana 500000, white sugar 200000, agar powder 54000, gac 20000, wherein except potato, banana, white sugar, agar powder, other materials beyond gac are all at room temperature dissolved in 2L water, after potato is ripe with 2L poach, blend, add in solution above, banana adds after directly blending, and gac directly adds and mixes, white sugar and agar powder are boiled to the rear dissolving of boiling in 3L water, add in mixing solutions above, after stirring, add water constant volume to 10L and regulate pH value to 5.4～5.5.
This substratum is sub-packed in tissue culture bottle, and 121 ℃ of temperature, high pressure 25 minutes, to be cooledly solidifies rear access butterfly orchid seedling and cultivates.After 15 days, blade is loose, dark green, and root system is healthy and strong without sticking up root phenomenon, demonstrates good strengthening seedling and rooting effect.
Embodiment 2:
Preparation 10L butterfly orchid strengthening seedling and rooting substratum, take the following material in milligram: Calcium Chloride Powder Anhydrous 3000, nitric acid ammonia 15000, saltpetre 7000, anhydrous magnesium sulfate 800, potassium primary phosphate 700, manganous sulfate 100, zinc sulfate 30, copper sulfate 0.1, cobalt chloride 0.1, Sodium orthomolybdate 1, boric acid 20, potassiumiodide 1, disodium ethylene diamine tetraacetate 300, ferrous sulfate 200, VITMAIN B1 0.5, nicotinic acid 1, vitamin B6 1, inositol 500, glycine 10, naphthylacetic acid 0.5, potato 300000, banana 300000, white sugar 100000, agar powder 48000, gac 10000, wherein except potato, banana, white sugar, agar powder, other materials beyond gac are all dissolved in 2L water, after potato is ripe with 2L poach, blend, add in solution above, banana adds after directly blending, and gac directly adds and mixes, white sugar and agar powder are boiled to the rear dissolving of boiling in 3L water, add in mixing solutions above, after stirring, add water constant volume to 10L and regulate pH value to 5.4～5.5.
This substratum is sub-packed in tissue culture bottle, and at 121 ℃ of temperature, high pressure 25 minutes, to be cooledly solidifies rear access butterfly orchid seedling and cultivates.After 15 days, blade is loose, dark green, and root system is healthy and strong without sticking up root phenomenon, demonstrates good strengthening seedling and rooting effect.
Embodiment 3:
Preparation 10L butterfly orchid strengthening seedling and rooting substratum, take the following material in milligram: Calcium Chloride Powder Anhydrous 4000, nitric acid ammonia 17000, saltpetre 10000, anhydrous magnesium sulfate 1000, potassium primary phosphate 1000, manganous sulfate 200, zinc sulfate 60, copper sulfate 1, cobalt chloride 1, Sodium orthomolybdate 10, boric acid 50, potassiumiodide 10, disodium ethylene diamine tetraacetate 500, ferrous sulfate 400, vitamin B15, nicotinic acid 10, vitamin B6 10, inositol 2000, glycine 50, naphthylacetic acid 10, potato 600000, banana 600000, white sugar 300000, agar powder 60000, gac 30000, wherein except potato, banana, white sugar, agar powder, other materials beyond gac are all dissolved in 2L water, after potato is ripe with 2L poach, blend, add in solution above, banana adds after directly blending, and gac directly adds and mixes, white sugar and agar powder are boiled to the rear dissolving of boiling in 3L water, add in mixing solutions above, after stirring, add water constant volume to 10L and regulate pH value to 5.4～5.5.
This substratum is sub-packed in tissue culture bottle, and at 121 ℃ of temperature, high pressure 25 minutes, to be cooledly solidifies rear access butterfly orchid seedling and cultivates.After 15 days, blade is loose, dark green, and root system is healthy and strong without sticking up root phenomenon, demonstrates good strengthening seedling and rooting effect.
Embodiment 4
Preparation 10L butterfly orchid strengthening seedling and rooting substratum, take the following material in milligram: Calcium Chloride Powder Anhydrous 3200, nitric acid ammonia 16000, saltpetre 8000, anhydrous magnesium sulfate 900, potassium primary phosphate 750, manganous sulfate 100, zinc sulfate 40, copper sulfate 0.1, cobalt chloride 0.1, Sodium orthomolybdate 1, boric acid 20, potassiumiodide 1, disodium ethylene diamine tetraacetate 300, ferrous sulfate 200, VITMAIN B1 0.5, nicotinic acid 1, vitamin B6 1, inositol 500, glycine 10, naphthylacetic acid 0.5, potato 400000, banana 400000, white sugar 150000, agar powder 50000, gac 15000, wherein except potato, banana, white sugar, agar powder, other materials beyond gac are all dissolved in 2L water, after potato is ripe with 2L poach, blend, add in solution above, banana adds after directly blending, and gac directly adds and mixes, white sugar and agar powder are boiled to the rear dissolving of boiling in 3L water, add in mixing solutions above, after stirring, add water constant volume to 10L and regulate pH value to 5.4～5.5.
This substratum is sub-packed in tissue culture bottle, and at 125 ℃ of temperature, high pressure 30 minutes, to be cooledly solidifies rear access butterfly orchid seedling and cultivates.After 15 days, blade is loose, dark green, and root system is healthy and strong without sticking up root phenomenon, demonstrates good strengthening seedling and rooting effect.
Embodiment 5
Preparation 10L butterfly orchid strengthening seedling and rooting substratum, take the following material in milligram: Calcium Chloride Powder Anhydrous 3500, nitric acid ammonia 16800, saltpetre 9500, anhydrous magnesium sulfate 950, potassium primary phosphate 950, manganous sulfate 150, zinc sulfate 60, copper sulfate 0.5, cobalt chloride 0.5, Sodium orthomolybdate 5, boric acid 40, potassiumiodide 5, disodium ethylene diamine tetraacetate 400, ferrous sulfate 300, VITMAIN B1 3, nicotinic acid 8, vitamin B6 8, inositol 1500, glycine 30, naphthylacetic acid 5, potato 550000, banana 550000, white sugar 250000, agar powder 55000, gac 25000, wherein except potato, banana, white sugar, agar powder, other materials beyond gac are all dissolved in 2L water, after potato is ripe with 2L poach, blend, add in solution above, banana adds after directly blending, and gac directly adds and mixes, white sugar and agar powder are boiled to the rear dissolving of boiling in 3L water, add in mixing solutions above, after stirring, add water constant volume to 10L and regulate pH value to 5.4～5.5.
This substratum is sub-packed in tissue culture bottle, and at 123 ℃ of temperature, high pressure 25 minutes, to be cooledly solidifies rear access butterfly orchid seedling and cultivates.After 15 days, blade is loose, dark green, and root system is healthy and strong without sticking up root phenomenon, demonstrates good strengthening seedling and rooting effect.
Claims (1)
1. a moth orchid culture medium, is characterized in that: substratum is to take water as solvent, composed as follows in mg/L:
1), Calcium Chloride Powder Anhydrous 332
2), nitric acid ammonia 1650
3), saltpetre 950
4), anhydrous magnesium sulfate 90.5
5), potassium primary phosphate 85
6), disodium ethylene diamine tetraacetate 37.3
7), ferrous sulfate 27.8
8), manganous sulfate 11.15
9), zinc sulfate 4.3
10), copper sulfate 0.0125
11), cobalt chloride 0.0125
12), Sodium orthomolybdate 0.125
13), boric acid 3.1
14), potassiumiodide 0.42
15), VITMAIN B1 0.1
16), nicotinic acid 0.5
17), vitamin B6 0.5
18), inositol 100
19), glycine 2
20), naphthylacetic acid 0.3
21), potato 50000
22), banana 50000
23), white sugar 20000
24), agar powder 5400
25), gac 2000.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010190129.5A CN101857496B (en) | 2010-06-02 | 2010-06-02 | Moth orchid culture medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010190129.5A CN101857496B (en) | 2010-06-02 | 2010-06-02 | Moth orchid culture medium |
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| CN101857496A CN101857496A (en) | 2010-10-13 |
| CN101857496B true CN101857496B (en) | 2014-04-16 |
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| CN102067778B (en) * | 2010-10-15 | 2012-06-27 | 天津滨海国际花卉科技园区股份有限公司 | Method for preventing and controlling phalaenopsis amabilis brown spot |
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| CN102613076A (en) * | 2012-03-26 | 2012-08-01 | 吴海红 | Vegetative propagation method for butterfly orchid |
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| CN103814819B (en) * | 2013-12-11 | 2015-07-08 | 柳州赛特生物科技研发中心 | Culture medium special for tissue culture of phalaenopsis amabilis |
| CN103814818B (en) * | 2013-12-11 | 2015-07-08 | 柳州赛特生物科技研发中心 | Culture medium special for tissue culture of phalaenopsis amabilis |
| CN103814817B (en) * | 2013-12-11 | 2015-07-08 | 柳州赛特生物科技研发中心 | Culture medium special for tissue culture of phalaenopsis amabilis |
| CN105085080A (en) * | 2015-09-16 | 2015-11-25 | 段宝荣 | Nutrient solution used for indoor flower arrangement and preparation method thereof |
| CN107382543B (en) * | 2017-09-11 | 2020-12-22 | 张芬 | Plant culture medium integrating disease, insect and fertilizer increasing effects and preparation process thereof |
| CN107602216B (en) * | 2017-09-11 | 2020-12-22 | 张芬 | Plant growth regulating base |
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| CN109662017A (en) * | 2019-02-14 | 2019-04-23 | 芜湖东源新农村开发股份有限公司 | The method for improving iris sprigging survival rate |
| CN109769646B (en) * | 2019-03-31 | 2021-09-17 | 芜湖东源新农村开发股份有限公司 | Cultivation method for prolonging flowering phase of butterfly orchid |
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| CN1180105A (en) * | 1996-10-09 | 1998-04-29 | 中国石化扬子石油化工公司 | Iris tissue culture media composition |
| CN1631103A (en) * | 2004-12-24 | 2005-06-29 | 中国科学院武汉植物园 | Rapid breeding method of Changnienia amoena Chien |
| CN101578960A (en) * | 2009-06-24 | 2009-11-18 | 华中农业大学 | Method for reducing aerial root of tissue culture seedling of hydrangea |
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2010
- 2010-06-02 CN CN201010190129.5A patent/CN101857496B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1180105A (en) * | 1996-10-09 | 1998-04-29 | 中国石化扬子石油化工公司 | Iris tissue culture media composition |
| CN1631103A (en) * | 2004-12-24 | 2005-06-29 | 中国科学院武汉植物园 | Rapid breeding method of Changnienia amoena Chien |
| CN101578960A (en) * | 2009-06-24 | 2009-11-18 | 华中农业大学 | Method for reducing aerial root of tissue culture seedling of hydrangea |
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