CN103814817B - Culture medium special for tissue culture of phalaenopsis amabilis - Google Patents
Culture medium special for tissue culture of phalaenopsis amabilis Download PDFInfo
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- CN103814817B CN103814817B CN201310669062.7A CN201310669062A CN103814817B CN 103814817 B CN103814817 B CN 103814817B CN 201310669062 A CN201310669062 A CN 201310669062A CN 103814817 B CN103814817 B CN 103814817B
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- culture medium
- substratum
- butterfly orchid
- juice
- culture
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a culture medium special for tissue culture of phalaenopsis amabilis. The culture medium comprises an MS culture medium and further comprises the following components: 1.0-1.2 g/L of grapefruitjuice, 0.3-0.33 g/L of tomato juice and 1-1.3 g/l of polyvinylpyrrolidone. The culture medium disclosed by the invention is used for culturing 'Mount Lip' phalaenopsis amabilis, and the survival rate is 98-99 percent and the microbiological contamination rate is 2.2-2.4 percent; the browning degree of the culture medium disclosed by the invention is 25-28 percent lower than that of the MS culture medium.
Description
Technical field
The invention belongs to technical field of plant culture, be specifically related to one " White Galaxy " kind butterfly orchid private organizations culture medium.
Background technology
Butterfly orchid has another name called phalaenopsis (Phalaenopsis amabilis), the orchid family (Orchidaceae) phalaenopsis belongs to (Phalaenopsis) perennial herbaceous plant that grows nonparasitically upon another plant, there is the butterfly orchid of the title of " cattleya queen ", because its flower shape such as butterfly with different colors dances in the air, beautiful in colour, with a slim and graceful figure, elegant, extremely welcome on flowers market at home and abroad, always be the favorite of human consumer.And butterfly orchid is except potted plant, being also suitable for especially being used as cut-flower, is the top grade flower material of artistic flower arrangement.
It is use tissue culture vegetative propagation that current orchid in factory produces conventional method, i.e. so-called clonal propagation, have that breeding is fast, quantity greatly and the advantage of seedling variety pure.Utilize the explants such as butterfly orchid bennet lateral bud, blade, stem apex, be inoculated into after sterile-processed on substratum, nutrition tooth or protocorm can be induced.Breed in a large number in substratum further again, differentiation culture, so just can turn out a large amount of plant.Grow by the butterfly orchid seedling of clonal propagation, bloom and compare neat and consistent, the pattern proterties of maternal plant can be preserved completely.
In the Induction Process of protocorm, the butterfly orchid of different varieties and the selection of explant to optimum medium different." White Galaxy " belongs to the one in butterfly orchid white-flowered varieties, formal name used at school Dtps.Mount Lip, and obstruct high 40 centimetres, Hua Jing 10.5 centimetres, spends band blush in vain, has businessman's called after " not mature maiden ".
Summary of the invention
The invention provides one " White Galaxy " kind butterfly orchid private organizations culture medium, be applicable to the cultivation of the butterfly orchid of " White Galaxy " kind.
The invention provides one " White Galaxy " kind butterfly orchid private organizations culture medium, comprise MS substratum, described substratum also comprises following component: Fructus Citri grandis juice 1.0-1.2g/L, tomato juice 0.3-0.33g/L, polyvinylpyrrolidone 1-1.3g/L.
Preferably, following component is also comprised in described substratum: pear juice 300-320mg/L.
Further, in described substratum, pear juice is 310mg/L.
Apply substratum of the present invention to carry out cultivating " White Galaxy " kind butterfly orchid, surviving rate is 98-99%, and microbiological contamination rate is 2.2-2.4%, and medium browning degree is the low 25-28% of MS substratum comparatively.
Embodiment
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.
Embodiment 1
One provided by the invention " White Galaxy " kind butterfly orchid private organizations culture medium formula is as follows:
" White Galaxy " of the present invention kind butterfly orchid private organizations culture medium improves to form on the basis of MS substratum, and concrete formula is as follows:
KNO
3：1900mg/L；
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na
2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Vitamin: 0.1mg/L;
Pyridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 30g/L;
Fructus Citri grandis juice: 1.1g/L;
Tomato juice: 0.31g/L;
Polyvinylpyrrolidone: 1.1g/L;
Agar: 7g/L.
Embodiment 2
One provided by the invention " White Galaxy " kind butterfly orchid private organizations culture medium formula is as follows:
" White Galaxy " of the present invention kind butterfly orchid private organizations culture medium improves to form on the basis of MS substratum, and concrete formula is as follows:
KNO
3：1900mg/L；
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na
2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Vitamin: 0.1mg/L;
Pyridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 30g/L;
Fructus Citri grandis juice: 1.2g/L;
Tomato juice: 0.33g/L;
Pear juice: 310mg/L;
Polyvinylpyrrolidone: 1.0g/L;
Agar: 7g/L.
Embodiment 3
One provided by the invention " White Galaxy " kind butterfly orchid private organizations culture medium formula is as follows:
" White Galaxy " of the present invention kind butterfly orchid private organizations culture medium improves to form on the basis of MS substratum, and concrete formula is as follows:
KNO
3：1900mg/L；
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na
2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Vitamin: 0.1mg/L;
Pyridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 30g/L;
Fructus Citri grandis juice: 1.0g/L;
Tomato juice: 0.30g/L;
Pear juice: 300mg/L;
Polyvinylpyrrolidone: 1.3g/L;
Agar: 7g/L.
Embodiment 4
One provided by the invention " White Galaxy " kind butterfly orchid private organizations culture medium formula is as follows:
" White Galaxy " of the present invention kind butterfly orchid private organizations culture medium improves to form on the basis of MS substratum, and concrete formula is as follows:
KNO
3：1900mg/L；
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na
2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Vitamin: 0.1mg/L;
Pyridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 30g/L;
Fructus Citri grandis juice: 1.2g/L;
Tomato juice: 0.32g/L;
Pear juice: 320mg/L;
Polyvinylpyrrolidone: 1.2g/L;
Agar: 7g/L.
Apply above-mentioned substratum and tissue culture carried out to butterfly orchid:
(1) cut butterfly orchid stem with bud, be about 2-3cm, clean up with tap water, in saturated chlorinated lime supernatant liquor, soak 15min;
(2) constantly stir when soaking, the stem section after immersion is clean with running water, is placed on Bechtop, first uses the alcohol disinfecting 30s of 75%, sterile water wash 1 time, then soaks 10min with the mercuric chloride of 0.1%;
(3) through aseptic water washing for several times, be inoculated into by stem with bud on substratum, adjust pH to be 4.5, illumination every day 12 hours, light intensity 1800Lux, cultivated at temperature 26 DEG C;
(4) constantly transfer according to 35 days one-period, each switching all can have the propagation of average 3 times, illumination every day 12 hours, light intensity 1800Lux, temperature 26 DEG C;
(5) seedling is divided into individual plant, is seeded in strengthening seedling and rooting substratum and cultivates, make it grow tall and grow up, grow flourishing root system simultaneously, final cultivation becomes a whole plant having root to have leaf, illumination every day 12 hours, light intensity 1800Lux, temperature 26 DEG C.
Experimental result: apply substratum of the present invention and carry out cultivating " White Galaxy " kind butterfly orchid, surviving rate is 98-99%, and microbiological contamination rate is 2.2-2.4%, and medium browning degree is the low 25-28% of MS substratum comparatively.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. " White Galaxy " kind butterfly orchid private organizations culture medium, comprise MS substratum, it is characterized in that: described substratum also comprises following component: Fructus Citri grandis juice 1.0-1.2g/L, tomato juice 0.3-0.33g/L, polyvinylpyrrolidone 1-1.3g/L, pear juice 300-320mg/L.
2. substratum according to claim 1, is characterized in that: in described substratum, pear juice is 310mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310669062.7A CN103814817B (en) | 2013-12-11 | 2013-12-11 | Culture medium special for tissue culture of phalaenopsis amabilis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310669062.7A CN103814817B (en) | 2013-12-11 | 2013-12-11 | Culture medium special for tissue culture of phalaenopsis amabilis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103814817A CN103814817A (en) | 2014-05-28 |
| CN103814817B true CN103814817B (en) | 2015-07-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310669062.7A Expired - Fee Related CN103814817B (en) | 2013-12-11 | 2013-12-11 | Culture medium special for tissue culture of phalaenopsis amabilis |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993954A (en) * | 2016-05-31 | 2016-10-12 | 徐振飞 | Phalaenopsis spp. rooting and seedling strengthening method |
| CN108419676A (en) * | 2018-03-30 | 2018-08-21 | 内蒙古自治区生物技术研究院 | Iris tissue culture medium (TCM) and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101857496B (en) * | 2010-06-02 | 2014-04-16 | 福建新世景园艺有限公司 | Moth orchid culture medium |
| CN103125380A (en) * | 2011-11-30 | 2013-06-05 | 江苏美尚生态景观股份有限公司 | Method for restraining butterfly orchid tissue from browning |
| CN103202233B (en) * | 2013-04-28 | 2014-06-11 | 安徽农业大学 | Butterfly orchid anti-browning tissue culture method and anti-browning culture medium |
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