CN103766217B - A kind of butterfly orchid private organizations culture medium - Google Patents
A kind of butterfly orchid private organizations culture medium Download PDFInfo
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- CN103766217B CN103766217B CN201310668855.7A CN201310668855A CN103766217B CN 103766217 B CN103766217 B CN 103766217B CN 201310668855 A CN201310668855 A CN 201310668855A CN 103766217 B CN103766217 B CN 103766217B
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- substratum
- butterfly orchid
- culture medium
- private organizations
- butterfly
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- Cultivation Of Plants (AREA)
Abstract
The invention provides a kind of butterfly orchid private organizations culture medium, comprise MS substratum, described substratum also comprises following component: 2,4-dichlorphenoxyacetic acid 0.1g/L, naphthylacetic acid 0.1g/L, 6-benzyl purine 0.1g/L, pear juice 1.0-1.2g/L, gac 0.3-0.4g/L.Apply substratum of the present invention to carry out cultivating " V31 " kind butterfly orchid, surviving rate is 98-99%, and microbiological contamination rate is 2.0-2.2%, and medium browning degree is the low 10-20% of MS substratum comparatively.
Description
Technical field
The invention belongs to technical field of plant culture, be specifically related to one " V31 " kind butterfly orchid private organizations culture medium.
Background technology
Butterfly orchid has another name called phalaenopsis (Phalaenopsis amabilis), the orchid family (Orchidaceae) phalaenopsis belongs to (Phalaenopsis) perennial herbaceous plant that grows nonparasitically upon another plant, there is the butterfly orchid of the title of " cattleya queen ", because its flower shape such as butterfly with different colors dances in the air, beautiful in colour, with a slim and graceful figure, elegant, extremely welcome on flowers market at home and abroad, always be the favorite of human consumer.And butterfly orchid is except potted plant, being also suitable for especially being used as cut-flower, is the top grade flower material of artistic flower arrangement.
It is use tissue culture vegetative propagation that current orchid in factory produces conventional method, i.e. so-called clonal propagation, have that breeding is fast, quantity greatly and the advantage of seedling variety pure.Utilize the explants such as butterfly orchid bennet lateral bud, blade, stem apex, be inoculated into after sterile-processed on substratum, nutrition tooth or protocorm can be induced.Breed in a large number in substratum further again, differentiation culture, so just can turn out a large amount of plant.Grow by the butterfly orchid seedling of clonal propagation, bloom and compare neat and consistent, the pattern proterties of maternal plant can be preserved completely.
In the Induction Process of protocorm, the butterfly orchid of different varieties and the selection of explant to optimum medium different." V31 " is a kind of butterfly orchid safflower series, with the Peduncle Length of overlength, and good inflorescence arrangement, the colored shape of the golden mean of the Confucian school and pattern, special flower motif, wins the welcome of the numerous producers and human consumer, for many years pursued, be the outstanding person in butterfly orchid always.
Summary of the invention
The invention provides one " V31 " kind butterfly orchid private organizations culture medium, be applicable to the cultivation of the butterfly orchid of " V31 " kind.
The invention provides one " V31 " kind butterfly orchid private organizations culture medium, comprise MS substratum, described substratum also comprises following component: 2,4-dichlorphenoxyacetic acid 0.1g/L, naphthylacetic acid 0.1g/L, 6-benzyl purine 0.1g/L, pear juice 1.0-1.2g/L, gac 0.3-0.4g/L.
Preferably, following component is also comprised in described substratum: Sucus Cocois 30-40mg/L.
Further, in described substratum, Sucus Cocois is 35mg/L.
Preferably, in described substratum, the concentration of sucrose is 20-25g/L.
Apply substratum of the present invention to carry out cultivating " V31 " kind butterfly orchid, surviving rate is 98-99%, and microbiological contamination rate is 2.0-2.2%, and medium browning degree is the low 10-20% of MS substratum comparatively.
Embodiment
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.
Embodiment 1
One provided by the invention " V31 " kind butterfly orchid special culture media formula is as follows:
" V31 " of the present invention kind butterfly orchid special culture media improves to form on the basis of MS substratum, and concrete formula is as follows: KNO
3: 1900mg/L;
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na
2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Vitamin: 0.1mg/L;
Pyridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 30g/L;
Agar: 7g/L;
2,4 dichlorophenoxyacetic acid 0.1g/L;
Naphthylacetic acid 0.1g/L;
6-benzyl purine 0.1g/L;
Pear juice 1.1g/L;
Gac 0.35g/L.
Embodiment 2
One provided by the invention " V31 " kind butterfly orchid special culture media formula is as follows:
" V31 " of the present invention kind butterfly orchid special culture media improves to form on the basis of MS substratum, and concrete formula is as follows: KNO
3: 1900mg/L;
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na
2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Vitamin: 0.1mg/L;
Pyridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 30g/L;
Agar: 7g/L;
2,4 dichlorophenoxyacetic acid 0.1g/L;
Naphthylacetic acid 0.1g/L;
6-benzyl purine 0.1g/L;
Pear juice 1.0g/L;
Sucus Cocois 35mg/L;
Gac 0.4g/L.Embodiment 3
One provided by the invention " V31 " kind butterfly orchid special culture media formula is as follows:
" V31 " of the present invention kind butterfly orchid special culture media improves to form on the basis of MS substratum, and concrete formula is as follows: KNO
3: 1900mg/L;
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Vitamin: 0.1mg/L;
Pyridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 20g/L;
Agar: 7g/L;
2,4 dichlorophenoxyacetic acid 0.1g/L;
Naphthylacetic acid 0.1g/L;
6-benzyl purine 0.1g/L;
Pear juice 1.2g/L;
Sucus Cocois 30mg/L;
Gac 0.3g/L.
Apply above-mentioned substratum and tissue culture carried out to butterfly orchid:
(1) cut butterfly orchid stem with bud, be about 2-3cm, clean up with tap water, in saturated chlorinated lime supernatant liquor, soak 15min;
(2) constantly stir when soaking, the stem section after immersion is clean with running water, is placed on Bechtop, first uses the alcohol disinfecting 30s of 75%, sterile water wash 1 time, then soaks 10min with the mercuric chloride of 0.1%;
(3) through aseptic water washing for several times, be inoculated into by stem with bud on substratum, adjust pH to be 4.5, illumination every day 12 hours, light intensity 1600Lux, cultivated at temperature 25 DEG C;
(4) constantly transfer according to 35 days one-period, each switching all can have the propagation of average 3 times, illumination every day 12 hours, light intensity 1600Lux, temperature 25 DEG C;
(5) seedling is divided into individual plant, is seeded in strengthening seedling and rooting substratum and cultivates, make it grow tall and grow up, grow flourishing root system simultaneously, final cultivation becomes a whole plant having root to have leaf, illumination every day 12 hours, light intensity 1600Lux, temperature 25 DEG C.
Experimental result: apply substratum of the present invention and carry out cultivating " V31 " kind butterfly orchid, surviving rate is 98-99%, and microbiological contamination rate is 2.0-2.2%, and medium browning degree is the low 10-20% of MS substratum comparatively.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. a butterfly orchid private organizations culture medium, comprises MS substratum, it is characterized in that: described substratum also comprises following component: 2,4-dichlorphenoxyacetic acid 0.1g/L, naphthylacetic acid 0.1g/L, 6-benzyl purine 0.1g/L, pear juice 1.0-1.2g/L, gac 0.3-0.4g/L.
2. substratum according to claim 1, is characterized in that: also comprise following component in described substratum: Sucus Cocois 30-40mg/L.
3. substratum according to claim 2, is characterized in that: in described substratum, Sucus Cocois is 35mg/L.
4. substratum according to claim 1, is characterized in that: in described substratum, the concentration of sucrose is 20-25g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310668855.7A CN103766217B (en) | 2013-12-11 | 2013-12-11 | A kind of butterfly orchid private organizations culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310668855.7A CN103766217B (en) | 2013-12-11 | 2013-12-11 | A kind of butterfly orchid private organizations culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103766217A CN103766217A (en) | 2014-05-07 |
| CN103766217B true CN103766217B (en) | 2015-10-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310668855.7A Expired - Fee Related CN103766217B (en) | 2013-12-11 | 2013-12-11 | A kind of butterfly orchid private organizations culture medium |
Country Status (1)
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419676A (en) * | 2018-03-30 | 2018-08-21 | 内蒙古自治区生物技术研究院 | Iris tissue culture medium (TCM) and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998046727A1 (en) * | 1997-04-15 | 1998-10-22 | University Of Massachusetts | Plant propagation compositions and methods |
| CN102577972A (en) * | 2012-03-12 | 2012-07-18 | 云南山里红生物科技有限公司 | Method for tissue culture of hoya kerrii |
| WO2012108518A1 (en) * | 2011-02-10 | 2012-08-16 | 国立大学法人広島大学 | Bacteriocin derived from lactobacillus rhamnosus |
| CN102845309A (en) * | 2012-09-29 | 2013-01-02 | 广东第二师范学院 | Method for efficiently regenerating plant through Hedychium coccineum Buch.-Ham somatic embryogenesis |
| CN103340155A (en) * | 2013-08-01 | 2013-10-09 | 武爱龙 | In-vitro tissue culture and rapid propagation method of Terminalia mantaly Tricolor |
-
2013
- 2013-12-11 CN CN201310668855.7A patent/CN103766217B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998046727A1 (en) * | 1997-04-15 | 1998-10-22 | University Of Massachusetts | Plant propagation compositions and methods |
| WO2012108518A1 (en) * | 2011-02-10 | 2012-08-16 | 国立大学法人広島大学 | Bacteriocin derived from lactobacillus rhamnosus |
| CN102577972A (en) * | 2012-03-12 | 2012-07-18 | 云南山里红生物科技有限公司 | Method for tissue culture of hoya kerrii |
| CN102845309A (en) * | 2012-09-29 | 2013-01-02 | 广东第二师范学院 | Method for efficiently regenerating plant through Hedychium coccineum Buch.-Ham somatic embryogenesis |
| CN103340155A (en) * | 2013-08-01 | 2013-10-09 | 武爱龙 | In-vitro tissue culture and rapid propagation method of Terminalia mantaly Tricolor |
Non-Patent Citations (1)
| Title |
|---|
| 吕晋慧等.天然复合物.《园艺植物组织培养》.2008,第18-19页，尤其是第19页第5段，第1段。. * |
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| CN103766217A (en) | 2014-05-07 |
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