CN103766217A - Special tissue culture medium for butterfly orchid - Google Patents
Special tissue culture medium for butterfly orchid Download PDFInfo
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- CN103766217A CN103766217A CN201310668855.7A CN201310668855A CN103766217A CN 103766217 A CN103766217 A CN 103766217A CN 201310668855 A CN201310668855 A CN 201310668855A CN 103766217 A CN103766217 A CN 103766217A
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- medium
- culture medium
- moth orchid
- tissue culture
- butterfly orchid
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Abstract
The invention provides a special tissue culture medium for butterfly orchid. The special tissue culture medium comprises an MS (Murashig and Skoog) culture medium and also comprises the following components: 0.1g/L 2,4-dichlorphenoxyacetic acid, 0.1g/L naphthylacetic acid, 0.1g/L6-benzylpurine, 1.0-1.2 g/L pear juice and 0.3-0.4 g/L active carbon. By adopting the culture medium disclosed by the invention to culture 'V31' breed of butterfly orchid, the survival rate is 98-99 percent, the contamination rate is 2.0-2.2 percent, and the browning degree of the culture medium is 10-20 percent lower than that of the MS culture medium.
Description
Technical field
The invention belongs to technical field of plant culture, be specifically related to one " V31 " kind Moth orchid private organizations culture medium.
Background technology
Moth orchid has another name called phalaenopsis (Phalaenopsis amabilis), the orchid family (Orchidaceae) phalaenopsis belongs to (Phalaenopsis) perennial herbaceous plant that grows nonparasitically upon another plant, there is the Moth orchid of the title of " cattleya queen ", because its flower shape is danced in the air as butterfly with different colors, beautiful in colour, with a slim and graceful figure, elegant, extremely welcome on flowers market at home and abroad, always be consumer's favorite.And Moth orchid is except potted plant, also suitable to especially cut-flower, be the top grade flower material of artistic flower arrangement.
At present to produce conventional method be to cultivate vegetative propagation with tissue to orchid in factory, i.e. so-called clonal propagation, has advantages of that breeding is fast, quantity is large and seedling variety pure.Utilize the explants such as Moth orchid bennet lateral bud, blade, stem apex, be inoculated on medium after sterile-processed, can induce nutrition tooth or protocorm.Further in medium, breed in a large number again, break up cultivation, so just can turn out a large amount of plant.By the Moth orchid seedling growth of clonal propagation, the comparison neat and consistent of blooming, can preserve the pattern proterties of maternal plant completely.
In the Induction Process of protocorm, the Moth orchid of different cultivars and explant are different to the selection of optimum medium." V31 " is a kind of Moth orchid safflower series, and with the bennet length of overlength, good inflorescence is arranged, colored shape and the pattern of the golden mean of the Confucian school, special flower motif, wins the numerous producers and consumer's welcome, for many years pursued, be the outstanding person in Moth orchid always.
Summary of the invention
The invention provides one " V31 " kind Moth orchid private organizations culture medium, be applicable to the cultivation of the Moth orchid of " V31 " kind.
The invention provides one " V31 " kind Moth orchid private organizations culture medium, comprise MS medium, described medium also comprises following component: 2,4-dichlorphenoxyacetic acid 0.1g/L, methyl α-naphthyl acetate 0.1g/L, 6-benzyl purine 0.1g/L, pear juice 1.0-1.2g/L, active carbon 0.3-0.4g/L.
Preferably, in described medium, also comprise following component: coconut milk 30-40mg/L.
Further, in described medium, coconut milk is 35mg/L.
Preferably, in described medium, the concentration of sucrose is 20-25g/L.
Apply medium of the present invention and cultivate " V31 " kind Moth orchid, survival rate is 98-99%, and microbiological contamination rate is 2.0-2.2%, and medium browning degree is compared with the low 10-20% of MS medium.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
One provided by the invention " V31 " kind Moth orchid special culture media formula is as follows:
" V31 " of the present invention kind Moth orchid special culture media is to improve and form on the basis of MS medium, and concrete formula is as follows: KNO
3: 1900mg/L;
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na
2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Thiamine hydrochloride: 0.1mg/L;
Puridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 30g/L;
Agar: 7g/L;
2,4-dichlorphenoxyacetic acid 0.1g/L;
Methyl α-naphthyl acetate 0.1g/L;
6-benzyl purine 0.1g/L;
Pear juice 1.1g/L;
Active carbon 0.35g/L.
Embodiment 2
One provided by the invention " V31 " kind Moth orchid special culture media formula is as follows:
" V31 " of the present invention kind Moth orchid special culture media is to improve and form on the basis of MS medium, and concrete formula is as follows: KNO
3: 1900mg/L;
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na
2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Thiamine hydrochloride: 0.1mg/L;
Puridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 30g/L;
Agar: 7g/L;
2,4-dichlorphenoxyacetic acid 0.1g/L;
Methyl α-naphthyl acetate 0.1g/L;
6-benzyl purine 0.1g/L;
Pear juice 1.0g/L;
Coconut milk 35mg/L;
Active carbon 0.4g/L.Embodiment 3
One provided by the invention " V31 " kind Moth orchid special culture media formula is as follows:
" V31 " of the present invention kind Moth orchid special culture media is to improve and form on the basis of MS medium, and concrete formula is as follows: KNO
3: 1900mg/L;
NH
4NO
3：1650mg/L；
KH
2PO
4：170mg/L；
MgSO
4·7H
2O：370mg/L；
CaCl
2·2H
2O：440mg/L；
KI：0.83mg/L；
H
3BO
3：6.2mg/L；
MnSO
4·4H
2O：22.3mg/L；
ZnSO
4·7H
2O：8.6mg/L；
Na2MoO
4·2H
2O：0.25mg/L；
CuSO
4·5H
2O：0.025mg/L；
CoCl
2·6H
2O：0.025mg/L；
Na
2·EDTA：37.25mg/L；
FeSO
4·7H
2O：27.85mg/L；
Inositol: 100mg/L;
Glycine: 2mg/L;
Thiamine hydrochloride: 0.1mg/L;
Puridoxine hydrochloride: 0.5mg/L;
Nicotinic acid: 0.5mg/L;
Sucrose: 20g/L;
Agar: 7g/L;
2,4-dichlorphenoxyacetic acid 0.1g/L;
Methyl α-naphthyl acetate 0.1g/L;
6-benzyl purine 0.1g/L;
Pear juice 1.2g/L;
Coconut milk 30mg/L;
Active carbon 0.3g/L.
Apply above-mentioned medium Moth orchid organized to cultivation:
(1) cut Moth orchid stem with bud, be about 2-3cm, clean up with running water, in saturated bleaching powder supernatant, soak 15min;
(2) while immersion, constantly stir, the stem section after immersion is rinsed well with flowing water, is placed on superclean bench, first uses 75% alcohol disinfecting 30s, sterile water wash 1 time, then with 0.1% mercuric chloride immersion 10min;
(3) through aseptic water washing for several times, stem with bud is inoculated on medium, adjusting pH is 4.5, and illumination every day 12 hours, light intensity 1600Lux, cultivated at 25 ℃ of temperature;
(4) constantly transfer according to 35 days one-periods, each switching all can have the propagation of average 3 times, illumination every day 12 hours, light intensity 1600Lux, 25 ℃ of temperature;
(5) seedling is divided into individual plant, is seeded in strengthening seedling and rooting medium and cultivates, it is grown tall and grow up, grow flourishing root system simultaneously, final cultivation becomes a whole plant that has root to have leaf, illumination every day 12 hours, light intensity 1600Lux, 25 ℃ of temperature.
Experimental result: apply medium of the present invention and cultivate " V31 " kind Moth orchid, survival rate is 98-99%, and microbiological contamination rate is 2.0-2.2%, and medium browning degree is compared with the low 10-20% of MS medium.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (4)
1. a Moth orchid private organizations culture medium, comprises MS medium, it is characterized in that: described medium also comprises following component: 2,4-dichlorphenoxyacetic acid 0.1g/L, methyl α-naphthyl acetate 0.1g/L, 6-benzyl purine 0.1g/L, pear juice 1.0-1.2g/L, active carbon 0.3-0.4g/L.
2. medium according to claim 1, is characterized in that: in described medium, also comprise following component: coconut milk 30-40mg/L.
3. medium according to claim 2, is characterized in that: in described medium, coconut milk is 35mg/L.
4. medium according to claim 1, is characterized in that: in described medium, the concentration of sucrose is 20-25g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310668855.7A CN103766217B (en) | 2013-12-11 | 2013-12-11 | A kind of butterfly orchid private organizations culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310668855.7A CN103766217B (en) | 2013-12-11 | 2013-12-11 | A kind of butterfly orchid private organizations culture medium |
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| Publication Number | Publication Date |
|---|---|
| CN103766217A true CN103766217A (en) | 2014-05-07 |
| CN103766217B CN103766217B (en) | 2015-10-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310668855.7A Expired - Fee Related CN103766217B (en) | 2013-12-11 | 2013-12-11 | A kind of butterfly orchid private organizations culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419676A (en) * | 2018-03-30 | 2018-08-21 | 内蒙古自治区生物技术研究院 | Iris tissue culture medium (TCM) and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998046727A1 (en) * | 1997-04-15 | 1998-10-22 | University Of Massachusetts | Plant propagation compositions and methods |
| CN102577972A (en) * | 2012-03-12 | 2012-07-18 | 云南山里红生物科技有限公司 | Method for tissue culture of hoya kerrii |
| WO2012108518A1 (en) * | 2011-02-10 | 2012-08-16 | 国立大学法人広島大学 | Bacteriocin derived from lactobacillus rhamnosus |
| CN102845309A (en) * | 2012-09-29 | 2013-01-02 | 广东第二师范学院 | Method for efficiently regenerating plant through Hedychium coccineum Buch.-Ham somatic embryogenesis |
| CN103340155A (en) * | 2013-08-01 | 2013-10-09 | 武爱龙 | In-vitro tissue culture and rapid propagation method of Terminalia mantaly Tricolor |
-
2013
- 2013-12-11 CN CN201310668855.7A patent/CN103766217B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998046727A1 (en) * | 1997-04-15 | 1998-10-22 | University Of Massachusetts | Plant propagation compositions and methods |
| WO2012108518A1 (en) * | 2011-02-10 | 2012-08-16 | 国立大学法人広島大学 | Bacteriocin derived from lactobacillus rhamnosus |
| CN102577972A (en) * | 2012-03-12 | 2012-07-18 | 云南山里红生物科技有限公司 | Method for tissue culture of hoya kerrii |
| CN102845309A (en) * | 2012-09-29 | 2013-01-02 | 广东第二师范学院 | Method for efficiently regenerating plant through Hedychium coccineum Buch.-Ham somatic embryogenesis |
| CN103340155A (en) * | 2013-08-01 | 2013-10-09 | 武爱龙 | In-vitro tissue culture and rapid propagation method of Terminalia mantaly Tricolor |
Non-Patent Citations (1)
| Title |
|---|
| 吕晋慧等: "《园艺植物组织培养》", 30 May 2008, article "天然复合物" * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419676A (en) * | 2018-03-30 | 2018-08-21 | 内蒙古自治区生物技术研究院 | Iris tissue culture medium (TCM) and preparation method thereof |
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| CN103766217B (en) | 2015-10-28 |
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