CN112352680A - Seedling-raising and rapid rooting method for anthurium tissue culture propagation - Google Patents
Seedling-raising and rapid rooting method for anthurium tissue culture propagation Download PDFInfo
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- CN112352680A CN112352680A CN202011319573.2A CN202011319573A CN112352680A CN 112352680 A CN112352680 A CN 112352680A CN 202011319573 A CN202011319573 A CN 202011319573A CN 112352680 A CN112352680 A CN 112352680A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a rapid seedling growing and rooting method for anthurium tissue culture propagation, which comprises the following steps: a. sterilizing the explant; b. callus induction culture; c. callus proliferation culture; d. rooting culture; e. hardening and transplanting the seedlings. Through the mode, the rapid rooting method for the seedling culture of the anthurium andraeanum tissue culture propagation can ensure rapid rooting and improve the survival rate of the anthurium andraeanum, and is simple, safe and reliable.
Description
Technical Field
The invention relates to the field of flower breeding, in particular to a rapid seedling rooting method for anthurium tissue culture propagation.
Background
Anthurium andraeanum (a. andraeanum) also called anthurium andraeanum, etc., is a perennial, evergreen, four season flowering, flower-viewing and leaf-viewing herbaceous plant belonging to the genus anthurium of the family of the Araceae, the native Columbia, and can be divided into two major categories, namely cut flowers and potted plants. Because of the characteristics of unique flower type, bright and colorful color, life and shade tolerance, long ornamental period and the like of anthurium andraeanum, the market consumption at home and abroad is very large, and the anthurium andraeanum is second to the orchid famous column in the global tropical flower trade.
Disclosure of Invention
The invention mainly solves the technical problem of providing a rapid rooting method for seedling culture of anthurium andraeanum tissue culture propagation, which can ensure rapid rooting and improve the survival rate of anthurium andraeanum, and is simple, safe and reliable.
In order to solve the technical problems, the invention adopts a technical scheme that: provides a rapid seedling growing and rooting method for anthurium tissue culture propagation, which comprises the following steps: a. and (3) explant sterilization: sterilizing with potassium permanganate solution, washing with sterile water for several times, soaking in 75% ethanol for 30-40 s, and washing with sterile water again until explant is clean; b. callus induction culture: transecting inflorescences pretreated at low temperature and disinfected by using a blade, stripping anthers by using a dissecting needle, inoculating the inflorescences into an induction culture medium, and carrying out induction culture for 4 months, wherein the sterile anthers are induced to generate callus and are removed in time for polluting the anthers; c. callus proliferation culture: transferring the callus generated by induction to a proliferation culture medium, and subculturing until the callus enters a proliferation period; d. rooting culture: inoculating the plantlet with roots to a rooting culture medium, controlling the temperature at 20 ℃, controlling the humidity in a greenhouse to be more than 70%, and controlling the illumination intensity: growing for one month in a 5000-8000Lux glass greenhouse, and supplementing the growth medium with 0mg/L IBA solution every 5 days; e. hardening and transplanting seedlings: and (3) putting the bottle seedlings inoculated in the rooting culture medium into a glass greenhouse for hardening seedlings, wherein the temperature is 20-28 ℃, and the illumination intensity is as follows: 5000 plus 6000Lux under the condition of natural light illumination duration, hardening the seedlings for 10 days, cleaning the seedlings with clear water at 20 ℃, planting the seedlings in a 105-hole plug tray, and culturing at the temperature of: 20-28 ℃, humidity: 70% -85%, illumination intensity: the growth in the glass greenhouse of 5000 plus 8000Lux can be recovered after 1 week, and the growth can be normal after 3 weeks.
In a preferred embodiment of the invention, the induction medium: 1/2MS minimal medium is added with 0.5-3mg/L2,4-D, 0.5-2mg/L6-BA, 30-70g/L sucrose, 20g/L glucose, 6g/L agar powder, 0.05mg/L kinetin and 1.0mg/L benzylamino adenine, and the pH is 5.8 before sterilization.
In a preferred embodiment of the invention, the multiplication medium: 1.0mg/L6-BA, 30g/L sucrose, 50mL/L coconut juice and 0.09g/L inositol are added into the MS minimal medium, and the pH is 5.8 before sterilization.
In a preferred embodiment of the invention, the rooting medium is 1/2MS minimal medium added with 1g/L of activated carbon, 13mg/L of plant polysaccharide and 1.1g/L of vermiculite powder, and the pH is 5.8 before sterilization.
The invention has the beneficial effects that: the rapid rooting method for the seedling culture of the anthurium andraeanum tissue culture propagation can ensure rapid rooting and improve the survival rate of the anthurium andraeanum, and is simple, safe and reliable.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A rapid rooting method for seedling culture of anthurium andraeanum tissue culture propagation comprises the following steps:
a. and (3) explant sterilization: sterilizing with potassium permanganate solution, washing with sterile water for several times, soaking in 75% ethanol for 30-40 s, and washing with sterile water again until explant is clean;
b. callus induction culture: transversely cutting the inflorescence pretreated at low temperature and disinfected by a blade, peeling off anthers by a dissecting needle, inoculating the inflorescence into an induction culture medium, and performing induction culture for 4 months, wherein the induction culture medium comprises the following components: 1/2MS basic culture medium is added with 0.5-3mg/L2,4-D, 0.5-2mg/L6-BA, 30-70g/L sucrose, 20g/L glucose, 6g/L agar powder, 0.05mg/L kinetin and 1.0mg/L benzylamino adenine, wherein the aseptic anther can induce to generate callus and can remove the polluted anther in time;
c. callus proliferation culture: transferring the callus generated by induction into a proliferation culture medium: adding 1.0mg/L6-BA, 30g/L sucrose, 50mL/L coconut juice, 0.09g/L inositol into MS minimal medium, performing subculture until callus enters proliferation stage, wherein the pH is 5.8L before sterilization, and the pH is 5.8 before sterilization;
d. rooting culture: inoculating the seedlings with roots to a rooting culture medium, wherein the rooting culture medium is 1/2MS basic culture medium added with 1g/L of activated carbon, 13mg/L of plant polysaccharide and 1.1g/L of vermiculite powder, the pH value is 5.8 before sterilization, the temperature is controlled at 20 ℃, the humidity in a greenhouse is more than 70 percent, and the illumination intensity is as follows: growing for one month in a 5000-8000Lux glass greenhouse, and supplementing the growth medium with 0mg/L IBA solution every 5 days;
e. hardening and transplanting seedlings: and (3) putting the bottle seedlings inoculated in the rooting culture medium into a glass greenhouse for hardening seedlings, wherein the temperature is 20-28 ℃, and the illumination intensity is as follows: 5000 plus 6000Lux under the condition of natural light illumination duration, hardening the seedlings for 10 days, cleaning the seedlings with clear water at 20 ℃, planting the seedlings in a 105-hole plug tray, and culturing at the temperature of: 20-28 ℃, humidity: 70% -85%, illumination intensity: the growth in the glass greenhouse of 5000 plus 8000Lux can be recovered after 1 week, and the growth can be normal after 3 weeks.
Compared with the prior art, the rapid rooting method for the seedlings in the anthurium tissue culture propagation can ensure rapid rooting and improve the survival rate of the anthurium, and is simple, safe and reliable.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (4)
1. A rapid rooting method for seedling culture of anthurium andraeanum tissue culture propagation is characterized by comprising the following steps:
a. and (3) explant sterilization: sterilizing with potassium permanganate solution, washing with sterile water for several times, soaking in 75% ethanol for 30-40 s, and washing with sterile water again until explant is clean;
b. callus induction culture: transecting inflorescences pretreated at low temperature and disinfected by using a blade, stripping anthers by using a dissecting needle, inoculating the inflorescences into an induction culture medium, and carrying out induction culture for 4 months, wherein the sterile anthers are induced to generate callus and are removed in time for polluting the anthers;
c. callus proliferation culture: transferring the callus generated by induction to a proliferation culture medium, and subculturing until the callus enters a proliferation period;
d. rooting culture: inoculating the plantlet with roots to a rooting culture medium, controlling the temperature at 20 ℃, controlling the humidity in a greenhouse to be more than 70%, and controlling the illumination intensity: growing for one month in a 5000-8000Lux glass greenhouse, and supplementing the growth medium with 0mg/L IBA solution every 5 days;
e. hardening and transplanting seedlings: and (3) putting the bottle seedlings inoculated in the rooting culture medium into a glass greenhouse for hardening seedlings, wherein the temperature is 20-28 ℃, and the illumination intensity is as follows: 5000 plus 6000Lux under the condition of natural light illumination duration, hardening the seedlings for 10 days, cleaning the seedlings with clear water at 20 ℃, planting the seedlings in a 105-hole plug tray, and culturing at the temperature of: 20-28 ℃, humidity: 70% -85%, illumination intensity: the growth in the glass greenhouse of 5000 plus 8000Lux can be recovered after 1 week, and the growth can be normal after 3 weeks.
2. The method for rapid rooting of seedlings for tissue culture and propagation of anthurium andraeanum according to claim 1, wherein the induction culture medium comprises: 1/2MS minimal medium is added with 0.5-3mg/L2,4-D, 0.5-2mg/L6-BA, 30-70g/L sucrose, 20g/L glucose, 6g/L agar powder, 0.05mg/L kinetin and 1.0mg/L benzylamino adenine, and the pH is 5.8 before sterilization.
3. The rapid rooting method for seedlings of anthurium andraeanum tissue culture propagation according to claim 1, characterized in that the propagation medium: 1.0mg/L6-BA, 30g/L sucrose, 50mL/L coconut juice and 0.09g/L inositol are added into the MS minimal medium, and the pH is 5.8 before sterilization.
4. The tissue culture propagation seedling rapid rooting method for anthurium andraeanum according to claim 1, characterized in that the rooting medium is 1/2MS minimal medium supplemented with 1g/L of activated carbon, 13mg/L of plant polysaccharide and 1.1g/L of vermiculite powder, and the pH is 5.8 before sterilization.
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| CN202011319573.2A CN112352680A (en) | 2020-11-23 | 2020-11-23 | Seedling-raising and rapid rooting method for anthurium tissue culture propagation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114287343A (en) * | 2022-01-05 | 2022-04-08 | 新疆维吾尔自治区阿克苏地区林业技术推广服务中心 | Tissue culture rapid propagation culture medium for anthurium andraeanum and tissue culture rapid propagation seed production method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104137777A (en) * | 2014-07-29 | 2014-11-12 | 浙江省萧山棉麻研究所 | Method for obtaining anthurium haplobionts |
| CN104904594A (en) * | 2015-05-19 | 2015-09-16 | 锡山区先锋家庭农场 | Anthurium adndraeanum pollen culture method |
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- 2020-11-23 CN CN202011319573.2A patent/CN112352680A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104137777A (en) * | 2014-07-29 | 2014-11-12 | 浙江省萧山棉麻研究所 | Method for obtaining anthurium haplobionts |
| CN104904594A (en) * | 2015-05-19 | 2015-09-16 | 锡山区先锋家庭农场 | Anthurium adndraeanum pollen culture method |
Non-Patent Citations (1)
| Title |
|---|
| 杜宝贵等: "红掌花药培养 ", 《生物技术通报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114287343A (en) * | 2022-01-05 | 2022-04-08 | 新疆维吾尔自治区阿克苏地区林业技术推广服务中心 | Tissue culture rapid propagation culture medium for anthurium andraeanum and tissue culture rapid propagation seed production method |
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