CN101855342B - 用于还原苯乙酮的酮还原酶多肽 - Google Patents

用于还原苯乙酮的酮还原酶多肽 Download PDF

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CN101855342B
CN101855342B CN2008801157703A CN200880115770A CN101855342B CN 101855342 B CN101855342 B CN 101855342B CN 2008801157703 A CN2008801157703 A CN 2008801157703A CN 200880115770 A CN200880115770 A CN 200880115770A CN 101855342 B CN101855342 B CN 101855342B
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CN101855342A (zh
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杰克·梁
史提芬尼·J·珍妮
艾米丽·穆德弗
沙琳·奇恩
约翰·M·格鲁伯
安克·克莱伯
古伽特·W·哈思曼
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01184—Carbonyl reductase (NADPH) (1.1.1.184)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Enzymes And Modification Thereof (AREA)
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CN2008801157703A 2007-09-13 2008-09-13 用于还原苯乙酮的酮还原酶多肽 Active CN101855342B (zh)

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US97205807P 2007-09-13 2007-09-13
US60/972,058 2007-09-13
PCT/US2008/076333 WO2009036404A2 (en) 2007-09-13 2008-09-13 Ketoreductase polypeptides for the reduction of acetophenones

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CN101855342B true CN101855342B (zh) 2013-07-10

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EP (1) EP2198018B1 (enExample)
JP (4) JP5973131B2 (enExample)
KR (1) KR101586503B1 (enExample)
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Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010536385A (ja) * 2007-08-24 2010-12-02 コデクシス， インコーポレイテッド （ｒ）−３−ヒドロキシチオランの立体選択的生成のための改善されたケトレダクターゼポリペプチド
KR101586503B1 (ko) * 2007-09-13 2016-01-18 코덱시스, 인코포레이티드 아세토페논의 환원을 위한 케토리덕타제 폴리펩티드
CN101889081B (zh) * 2007-09-28 2014-06-18 科德克希思公司 酮还原酶多肽及其用途
HUE027683T2 (en) 2007-10-01 2016-10-28 Codexis Inc Ketoreductase polypeptides for the production of azetidinone
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
WO2010025238A2 (en) 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010025287A2 (en) 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
SI2329014T1 (sl) 2008-08-29 2015-01-30 Codexis, Inc. Polipeptidi ketoreduktaze za stereoselektivno produkcijo (4s)-3(5s)-5(4-fluorofenil)-5-hidroksipentanoil)-4-fenil-1,3-oksazolidin -2-ona
WO2011022548A2 (en) 2009-08-19 2011-02-24 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
WO2011071982A2 (en) 2009-12-08 2011-06-16 Codexis, Inc. Synthesis of prazole compounds
HUE026367T2 (en) 2010-05-04 2016-06-28 Codexis Inc Biocatalysts for ezetimibe synthesis
DK2649187T3 (da) 2010-12-08 2018-01-29 Codexis Inc Biokatalysatorer og fremgangsmåder til syntesen af armodafinil
HUE038800T2 (hu) 2011-04-13 2018-11-28 Codexis Inc Biokatalitikus eljárás eszlikarbazepin és ennek analógjai elõállítására
ES2584427T3 (es) 2011-10-27 2016-09-27 Merck Sharp & Dohme Corp. Proceso para la preparación de agonistas beta 3 y productos intermedios
WO2013062881A1 (en) 2011-10-27 2013-05-02 Merck Sharp & Dohme Corp. Process for making beta 3 agonists and intermediates
CN106479990B (zh) 2011-11-18 2020-09-18 科德克希思公司 用于制备羟基取代的氨基甲酸酯的生物催化剂
WO2013170050A1 (en) 2012-05-11 2013-11-14 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
EP2850200A1 (en) * 2012-05-17 2015-03-25 Genentech, Inc. Process of making hydroxylated cyclopentapyrimidine compounds and salts thereof
JP6383403B2 (ja) 2013-03-15 2018-08-29 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． β−３作動薬および中間体の製造方法
US10150959B2 (en) 2013-03-15 2018-12-11 Merck Sharp & Dohme Corp. Immobilized ketoreductases and process for making and using immobilized ketoreductase
JP6521967B2 (ja) 2013-08-06 2019-05-29 イマーゴ バイオサイエンシーズ インコーポレイテッド 疾患の治療のためのｋｄｍ１ａ阻害剤
DK3049973T3 (en) 2013-09-27 2018-10-22 Codexis Inc AUTOMATED SCREENING OF ENZYM VARIENTS
CN106232619B (zh) 2013-11-13 2022-09-09 科德克希思公司 工程化的亚胺还原酶和用于酮和胺化合物的还原胺化的方法
CN105814199B (zh) * 2013-12-10 2019-12-13 天野酶株式会社 修饰型脂肪酶及其用途
CN104975048B (zh) * 2014-04-02 2018-07-24 中国科学院过程工程研究所 梅岭霉素合成基因簇中酮还原酶MeiF在手性芳香醇生产中的应用
CN121203988A (zh) 2014-04-22 2025-12-26 C-乐克塔股份有限公司 酮还原酶
CN107002050A (zh) 2014-11-25 2017-08-01 科德克希思公司 工程化亚胺还原酶和用于酮和胺化合物的还原胺化的方法
DK3256577T3 (da) 2015-02-10 2020-09-14 Codexis Inc Ketoreduktase polypeptider til syntesen af chirale forbindelser
JP6727216B2 (ja) 2015-02-12 2020-07-22 イマーゴ バイオサイエンシーズ インコーポレイテッド 疾患の治療のためのｋｄｍ１ａ阻害剤
CN104846025B (zh) * 2015-03-31 2018-06-01 浙江大学 一种制备(2s，3r)-2-苯甲酰氨甲基-3-羟基丁酸甲酯的方法
CN105906656B (zh) * 2016-05-17 2018-01-23 凯莱英医药集团（天津）股份有限公司 一种克唑替尼中间体的合成方法
CN106047950A (zh) * 2016-06-30 2016-10-26 尚科生物医药（上海）有限公司 一种(s)‑1‑(2,6‑二氯‑3‑氟苯基)乙醇的生物制备方法
CN111194306B (zh) 2016-08-16 2023-05-16 伊美格生物科学公司 用于制备kdm1a抑制剂的方法和过程
CN110268067A (zh) * 2016-08-16 2019-09-20 伊美格生物科学公司 用于产生立体异构纯的氨基环丙烷的组合物和方法
CN106636248A (zh) * 2016-12-21 2017-05-10 浙江海洋大学 一种利用羰基还原酶制备克唑替尼中间体的方法
JP7045725B2 (ja) 2017-04-27 2022-04-01 コデクシス， インコーポレイテッド ケトレダクターゼポリペプチドおよびポリヌクレオチド
KR20250099259A (ko) 2017-06-06 2025-07-01 유로반트 사이언시즈 게엠베하 과민성 방광의 치료를 위한 비베그론의 용도
CN109112166B (zh) * 2017-06-26 2023-08-15 弈柯莱生物科技(上海)股份有限公司 酶法制备替卡格雷中间体
EP3652328A1 (en) 2017-07-14 2020-05-20 c-LEcta GmbH Ketoreductases
GB201802383D0 (en) * 2018-02-14 2018-03-28 Givaudan Sa Process
CN108624603A (zh) * 2018-03-30 2018-10-09 河北省科学院生物研究所 一种(s)-羰基还原酶及其编码基因与应用
JP7450559B2 (ja) 2018-05-11 2024-03-15 イマーゴ バイオサイエンシーズ インコーポレイテッド 疾患の処置のためのｋｄｍ１ａ阻害剤
AU2019304216B2 (en) 2018-07-16 2022-06-02 Novartis Ag Chemical process for preparing phenylpiperidinyl indole derivatives
TW202038963A (zh) 2018-12-05 2020-11-01 瑞士商優洛凡特科學公司 維貝隆（ｖｉｂｅｇｒｏｎ）於治療患有良性前列腺增生之男性中膀胱過動症症狀之用途
CN109706191B (zh) * 2019-01-21 2022-09-09 南京欧信医药技术有限公司 一种托莫西汀中间体的酶催化合成方法
JP6844073B1 (ja) * 2019-04-19 2021-03-17 株式会社エーピーアイ コーポレーション （１ｒ，３ｒ）−３−（トリフルオロメチル）シクロヘキサン−１−オール及びその中間体の製造法
CN110218718A (zh) * 2019-06-19 2019-09-10 南京趣酶生物科技有限公司 固定化酮还原酶突变体及其在制备奈必洛尔手性醇中间体及其类似物中的应用
CA3155659A1 (en) * 2019-09-26 2021-04-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
CN111235123B (zh) * 2020-03-27 2020-10-27 长兴制药股份有限公司 一种醇溶液高浓度耐受的羰基还原酶及其应用
CN111718965A (zh) * 2020-06-17 2020-09-29 宁波酶赛生物工程有限公司 一种(2s,3s)-2,3-丁二醇的制备方法
CN115478059B (zh) * 2021-05-31 2025-04-11 尚科生物医药（上海）有限公司 一种酮还原酶突变体及其应用
IL322082A (en) * 2023-01-12 2025-09-01 Novartis Ag Engineered ketoreductase polypeptides
WO2026093943A1 (en) 2024-10-31 2026-05-07 Novartis Ag Chemical process for preparing phenylpiperidinyl indole derivatives
CN120624386B (zh) * 2025-06-12 2025-12-09 亳州学院 一种醇脱氢酶突变体及其合成覆盆子酮的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017135A1 (en) * 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4014573C1 (enExample) 1990-05-07 1991-10-10 Forschungszentrum Juelich Gmbh, 5170 Juelich, De
US5225339A (en) 1992-02-26 1993-07-06 The Scripps Research Institute Lactobacillus kefir alcohol dehydrogenase
JP3155107B2 (ja) 1993-01-12 2001-04-09 ダイセル化学工業株式会社 光学活性４−ハロ−３−ヒドロキシ酪酸エステルの製造方法
US5427933A (en) 1994-07-20 1995-06-27 Merck & Co., Inc. Reduction of phenylalkyl ketones to the corresponding (S)-hydroxy derivatives using mucor hiemalis IFO 5834
US5491077A (en) 1994-07-20 1996-02-13 Merck & Co., Inc. Microbial method
JPH08336393A (ja) 1995-04-13 1996-12-24 Mitsubishi Chem Corp 光学活性なγ−置換−β−ヒドロキシ酪酸エステルの製造法
GB9512837D0 (en) 1995-06-23 1995-08-23 Zeneca Ltd reduction of ketone groups
DE19610984A1 (de) 1996-03-21 1997-09-25 Boehringer Mannheim Gmbh Alkohol-Dehydrogenase und deren Verwendung zur enzymatischen Herstellung chiraler Hydroxyverbindungen
US5891685A (en) 1996-06-03 1999-04-06 Mitsubishi Chemical Corporation Method for producing ester of (S)-γ-halogenated-β-hydroxybutyric acid
DE19812004A1 (de) 1998-03-19 1999-09-30 Forschungszentrum Juelich Gmbh Dehydrogenasen mit verbesserter NAD-Abhängigkeit, deren Herstellung und Verwendung
WO1999057109A1 (en) 1998-04-30 1999-11-11 Kaneka Corporation Process for producing 6-cyanomethyl-1,3-dioxane-4-acetic acid derivatives
DE19857302C2 (de) 1998-12-14 2000-10-26 Forschungszentrum Juelich Gmbh Verfahren zur enantioselektiven Reduktion von 3,5-Dioxocarbonsäuren, deren Salze und Ester
AU1551701A (en) 1999-12-03 2001-06-12 Kaneka Corporation Novel carbonyl reductase, gene thereof and method of using the same
DE10037101A1 (de) 2000-07-27 2002-02-07 Degussa Rekombinant hergestellte Enzyme mit verbesserter NAD(H)-Akzeptanz
US7202070B2 (en) 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
HUP0400261A3 (en) 2001-03-22 2005-11-28 Bristol Myers Squibb Co Stereoselective reduction of substituted acetophenone
DE10119274A1 (de) * 2001-04-20 2002-10-31 Juelich Enzyme Products Gmbh Enzymatisches Verfahren zur enantioselektiven Reduktion von Ketoverbindungen
PT2476667E (pt) 2003-02-26 2014-09-18 Sugen Inc Compostos de aminoheteroarilo como inibidores da proteína quinase
JP4213524B2 (ja) 2003-04-17 2009-01-21 ダイセル化学工業株式会社 新規なカルボニル還元酵素、その酵素をコードするｄｎａを含むポリヌクレオチド、その製造方法、およびこれを利用した光学活性アルコールの製造方法
WO2005018579A2 (en) 2003-08-11 2005-03-03 Codexis, Inc. Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters
DE10345772A1 (de) 2003-10-01 2005-04-21 Basf Ag Verfahren zur Herstellung von 3-Methylamino-1-(thien-2-yl)-propan-1-ol
US20080050787A1 (en) 2003-12-02 2008-02-28 Hazuki Nagai Method for Manufacturing Optically Active Tetrahydrothiophene Derivative and Method for Crystallization of Optically Active Tetrahydrothiophene-3-Ol
JP4604524B2 (ja) * 2004-03-16 2011-01-05 味の素株式会社 変異型アルドラーゼ、並びにこれを用いた光学活性ｉｈｏｇ及び光学活性モナティンの製造方法
KR20080072105A (ko) 2004-08-26 2008-08-05 화이자 인코포레이티드 단백질 티로신 키나제 저해제 중간체의 제조를 위한거울상이성질선택적 생물학적 전환
EP1786777A1 (en) 2004-08-26 2007-05-23 Pfizer, Inc. Aminoheteroaryl compounds as protein tyrosine kinase inhibitors
CA2577937C (en) 2004-08-26 2010-12-21 Pfizer Inc. Pyrazole-substituted aminoheteroaryl compounds as protein kinase inhibitors
JP4242911B2 (ja) 2004-08-26 2009-03-25 ファイザー・インク プロテインキナーゼ阻害薬としてのエナンチオピュアなアミノヘテロアリール化合物
AT501496B1 (de) 2005-02-21 2007-03-15 Iep Gmbh Verfahren zur enantioselektiven enzymatischen reduktion von ketoverbindungen
WO2006130657A2 (en) 2005-05-31 2006-12-07 Bristol-Myers Squibb Company Stereoselective reduction process for the preparation of pyrrolotriazine compounds
AT502395B1 (de) 2005-07-27 2007-03-15 Iep Gmbh Oxidoreduktasen zur stereoselektiven reduktion von ketoverbindungen
WO2008042876A2 (en) 2006-10-02 2008-04-10 Codexis, Inc. Compositions and methods for producing stereoisomerically pure statins and synthetic intermediates therefor
CN101627116B (zh) 2007-02-08 2013-07-10 科德克希思公司 酮还原酶及其用途
JP2010536385A (ja) 2007-08-24 2010-12-02 コデクシス， インコーポレイテッド （ｒ）−３−ヒドロキシチオランの立体選択的生成のための改善されたケトレダクターゼポリペプチド
KR101586503B1 (ko) * 2007-09-13 2016-01-18 코덱시스, 인코포레이티드 아세토페논의 환원을 위한 케토리덕타제 폴리펩티드
CN101889081B (zh) 2007-09-28 2014-06-18 科德克希思公司 酮还原酶多肽及其用途
HUE027683T2 (en) 2007-10-01 2016-10-28 Codexis Inc Ketoreductase polypeptides for the production of azetidinone
WO2010025287A2 (en) 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
SI2329014T1 (sl) 2008-08-29 2015-01-30 Codexis, Inc. Polipeptidi ketoreduktaze za stereoselektivno produkcijo (4s)-3(5s)-5(4-fluorofenil)-5-hidroksipentanoil)-4-fenil-1,3-oksazolidin -2-ona

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017135A1 (en) * 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides

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