CN101855342B - 用于还原苯乙酮的酮还原酶多肽 - Google Patents

用于还原苯乙酮的酮还原酶多肽 Download PDF

Info

Publication number
CN101855342B
CN101855342B CN2008801157703A CN200880115770A CN101855342B CN 101855342 B CN101855342 B CN 101855342B CN 2008801157703 A CN2008801157703 A CN 2008801157703A CN 200880115770 A CN200880115770 A CN 200880115770A CN 101855342 B CN101855342 B CN 101855342B
Authority
CN
China
Prior art keywords
residues
polypeptide
amino acid
residue
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN2008801157703A
Other languages
English (en)
Chinese (zh)
Other versions
CN101855342A (zh
Inventor
杰克·梁
史提芬尼·J·珍妮
艾米丽·穆德弗
沙琳·奇恩
约翰·M·格鲁伯
安克·克莱伯
古伽特·W·哈思曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of CN101855342A publication Critical patent/CN101855342A/zh
Application granted granted Critical
Publication of CN101855342B publication Critical patent/CN101855342B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01184—Carbonyl reductase (NADPH) (1.1.1.184)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2008801157703A 2007-09-13 2008-09-13 用于还原苯乙酮的酮还原酶多肽 Active CN101855342B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97205807P 2007-09-13 2007-09-13
US60/972,058 2007-09-13
PCT/US2008/076333 WO2009036404A2 (en) 2007-09-13 2008-09-13 Ketoreductase polypeptides for the reduction of acetophenones

Publications (2)

Publication Number Publication Date
CN101855342A CN101855342A (zh) 2010-10-06
CN101855342B true CN101855342B (zh) 2013-07-10

Family

ID=40260827

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008801157703A Active CN101855342B (zh) 2007-09-13 2008-09-13 用于还原苯乙酮的酮还原酶多肽

Country Status (6)

Country Link
US (12) US8748143B2 (enExample)
EP (1) EP2198018B1 (enExample)
JP (4) JP5973131B2 (enExample)
KR (1) KR101586503B1 (enExample)
CN (1) CN101855342B (enExample)
WO (1) WO2009036404A2 (enExample)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101784669B (zh) 2007-08-24 2015-02-18 科德克希思公司 用于(r)-3-羟基四氢噻吩的立体选择性制备的改善的酮还原酶多肽
CN101855342B (zh) 2007-09-13 2013-07-10 科德克希思公司 用于还原苯乙酮的酮还原酶多肽
CN101889081B (zh) * 2007-09-28 2014-06-18 科德克希思公司 酮还原酶多肽及其用途
SI2205727T1 (sl) 2007-10-01 2015-09-30 Codexis, Inc. Polipeptidi ketoreduktaze za izdelavo azetidinona
US8288141B2 (en) 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8288131B2 (en) * 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides and uses thereof
SI2329013T1 (sl) 2008-08-27 2016-03-31 Codexis, Inc. Polipeptidi ketoreduktaze za proizvodnjo 3-aril-3-hidroksipropanamina iz 3-aril-3-ketopropanamina
EP2329014B1 (en) 2008-08-29 2014-10-22 Codexis, Inc. Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
WO2011022548A2 (en) 2009-08-19 2011-02-24 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
EP2510089B1 (en) 2009-12-08 2015-10-21 Codexis, Inc. Synthesis of prazole compounds
US9040262B2 (en) 2010-05-04 2015-05-26 Codexis, Inc. Biocatalysts for ezetimibe synthesis
HUE037616T2 (hu) 2010-12-08 2018-09-28 Codexis Inc Biokatalizátorok és eljárások armodafinil szintéziséhez
DK2697662T3 (en) 2011-04-13 2018-07-30 Codexis Inc BIOCATALYTIC PROCEDURE FOR PREPARING ESLICARBAZEPIN AND ANALOGUES THEREOF
WO2013062878A1 (en) 2011-10-27 2013-05-02 Merck Sharp & Dohme Corp. Process for making beta 3 angonists and intermediates
JP6063948B2 (ja) 2011-10-27 2017-01-18 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． ベータ３アゴニストおよび中間体を製造するプロセス
CN104053771B (zh) 2011-11-18 2016-11-09 科德克希思公司 用于制备羟基取代的氨基甲酸酯的生物催化剂
US9193957B2 (en) 2012-05-11 2015-11-24 Codexis, Inc. Engineered imine reductases and methods for the reductive animation of ketone and amine compounds
KR102123131B1 (ko) * 2012-05-17 2020-06-15 제넨테크, 인크. 하이드록실화 사이클로펜타피리미딘 화합물 및 이의 염의 제조 방법
LT2968269T (lt) 2013-03-15 2019-10-25 Merck Sharp & Dohme Beta 3 agonistų ir tarpinių junginių gamybos būdas
WO2014150633A1 (en) 2013-03-15 2014-09-25 Merck Sharp & Dohme Corp. Immobilized ketoreductases and process for making and using immobilized ketoreductase
AU2014306149B2 (en) 2013-08-06 2019-09-19 Imago Biosciences Inc. KDM1A inhibitors for the treatment of disease
CN105765592B (zh) 2013-09-27 2019-12-17 科德克希思公司 用于酶变体的自动筛选的方法、装置和系统
HUE053379T2 (hu) 2013-11-13 2021-06-28 Codexis Inc Génmódosított imin-reduktázok és eljárások keton és amin vegyületek reduktív aminálására
US10415023B2 (en) * 2013-12-10 2019-09-17 Amano Enzyme Inc. Modified lipase and use thereof
CN104975048B (zh) * 2014-04-02 2018-07-24 中国科学院过程工程研究所 梅岭霉素合成基因簇中酮还原酶MeiF在手性芳香醇生产中的应用
EP3134519B1 (en) 2014-04-22 2018-06-06 c-LEcta GmbH Ketoreductases
CA2968275C (en) 2014-11-25 2023-09-12 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
US10696953B2 (en) 2015-02-10 2020-06-30 Codexis, Inc. Ketoreductase polypeptides for the synthesis of chiral compounds
MX387224B (es) 2015-02-12 2025-03-18 Imago Biosciences Inc Inhibidores de kdm1a para el tratamiento de enfermedades.
CN104846025B (zh) * 2015-03-31 2018-06-01 浙江大学 一种制备(2s，3r)-2-苯甲酰氨甲基-3-羟基丁酸甲酯的方法
CN105906656B (zh) * 2016-05-17 2018-01-23 凯莱英医药集团（天津）股份有限公司 一种克唑替尼中间体的合成方法
CN106047950A (zh) * 2016-06-30 2016-10-26 尚科生物医药（上海）有限公司 一种(s)‑1‑(2,6‑二氯‑3‑氟苯基)乙醇的生物制备方法
WO2018035259A1 (en) 2016-08-16 2018-02-22 Imago Biosciences, Inc. Methods and processes for the preparation of kdm1a inhibitors
WO2018035249A1 (en) * 2016-08-16 2018-02-22 Imago Biosciences, Inc. Compositions and methods for producing stereoisomerically pure aminocyclopropanes
CN106636248A (zh) * 2016-12-21 2017-05-10 浙江海洋大学 一种利用羰基还原酶制备克唑替尼中间体的方法
CA3061133A1 (en) 2017-04-27 2018-11-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
EP4410295A3 (en) 2017-06-06 2024-10-16 Urovant Sciences GmbH Use of vibegron to treat overactive bladder
CN109112166B (zh) * 2017-06-26 2023-08-15 弈柯莱生物科技(上海)股份有限公司 酶法制备替卡格雷中间体
WO2019012095A1 (en) 2017-07-14 2019-01-17 C-Lecta Gmbh TCO-reductase
GB201802383D0 (en) * 2018-02-14 2018-03-28 Givaudan Sa Process
CN108624603A (zh) * 2018-03-30 2018-10-09 河北省科学院生物研究所 一种(s)-羰基还原酶及其编码基因与应用
IL279260B2 (en) 2018-05-11 2024-10-01 Imago Biosciences Inc Kdm1a inhibitors for the treatment of disease
KR20210032950A (ko) 2018-07-16 2021-03-25 노파르티스 아게 페닐피페리디닐 인돌 유도체를 제조하기 위한 화학적 방법
NZ774649A (en) 2018-12-05 2025-07-25 Urovant Sciences Gmbh Vibegron for the treatment of overactive bladder symptoms
CN109706191B (zh) * 2019-01-21 2022-09-09 南京欧信医药技术有限公司 一种托莫西汀中间体的酶催化合成方法
EP3922728B1 (en) 2019-04-19 2023-11-29 API Corporation Method for producing (1r,3r)-3-(trifluoromethyl)cyclohexan-1-ol and intermediate thereof
CN110218718A (zh) * 2019-06-19 2019-09-10 南京趣酶生物科技有限公司 固定化酮还原酶突变体及其在制备奈必洛尔手性醇中间体及其类似物中的应用
CN114555795B (zh) * 2019-09-26 2025-05-27 科德克希思公司 酮还原酶多肽及多核苷酸
CN111235123B (zh) * 2020-03-27 2020-10-27 长兴制药股份有限公司 一种醇溶液高浓度耐受的羰基还原酶及其应用
CN111718965A (zh) * 2020-06-17 2020-09-29 宁波酶赛生物工程有限公司 一种(2s,3s)-2,3-丁二醇的制备方法
CN115478059B (zh) * 2021-05-31 2025-04-11 尚科生物医药（上海）有限公司 一种酮还原酶突变体及其应用
CN120981566A (zh) * 2023-01-12 2025-11-18 诺华股份有限公司 工程化酮还原酶多肽
CN120624386B (zh) * 2025-06-12 2025-12-09 亳州学院 一种醇脱氢酶突变体及其合成覆盆子酮的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017135A1 (en) * 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4014573C1 (enExample) 1990-05-07 1991-10-10 Forschungszentrum Juelich Gmbh, 5170 Juelich, De
US5225339A (en) 1992-02-26 1993-07-06 The Scripps Research Institute Lactobacillus kefir alcohol dehydrogenase
JP3155107B2 (ja) 1993-01-12 2001-04-09 ダイセル化学工業株式会社 光学活性４−ハロ−３−ヒドロキシ酪酸エステルの製造方法
US5427933A (en) 1994-07-20 1995-06-27 Merck & Co., Inc. Reduction of phenylalkyl ketones to the corresponding (S)-hydroxy derivatives using mucor hiemalis IFO 5834
US5491077A (en) 1994-07-20 1996-02-13 Merck & Co., Inc. Microbial method
JPH08336393A (ja) 1995-04-13 1996-12-24 Mitsubishi Chem Corp 光学活性なγ−置換−β−ヒドロキシ酪酸エステルの製造法
GB9512837D0 (en) 1995-06-23 1995-08-23 Zeneca Ltd reduction of ketone groups
DE19610984A1 (de) 1996-03-21 1997-09-25 Boehringer Mannheim Gmbh Alkohol-Dehydrogenase und deren Verwendung zur enzymatischen Herstellung chiraler Hydroxyverbindungen
US5891685A (en) 1996-06-03 1999-04-06 Mitsubishi Chemical Corporation Method for producing ester of (S)-γ-halogenated-β-hydroxybutyric acid
DE19812004A1 (de) 1998-03-19 1999-09-30 Forschungszentrum Juelich Gmbh Dehydrogenasen mit verbesserter NAD-Abhängigkeit, deren Herstellung und Verwendung
US6344569B1 (en) 1998-04-30 2002-02-05 Kaneka Corporation Process for producing 6-cyanomethyl-1,3-dioxane-4-acetic acid derivatives
DE19857302C2 (de) 1998-12-14 2000-10-26 Forschungszentrum Juelich Gmbh Verfahren zur enantioselektiven Reduktion von 3,5-Dioxocarbonsäuren, deren Salze und Ester
SI20642A (sl) 1999-12-03 2002-02-28 Kaneka Corporation Nova karbonil-reduktaza, gen le-te in postopek za uporabo le-teh
DE10037101A1 (de) 2000-07-27 2002-02-07 Degussa Rekombinant hergestellte Enzyme mit verbesserter NAD(H)-Akzeptanz
US7202070B2 (en) 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
IL157386A0 (en) 2001-03-22 2004-02-19 Bristol Myers Squibb Co A stereoselective process for the preparation of (s)-1-arylethanol derivatives
DE10119274A1 (de) * 2001-04-20 2002-10-31 Juelich Enzyme Products Gmbh Enzymatisches Verfahren zur enantioselektiven Reduktion von Ketoverbindungen
DK1603570T5 (da) 2003-02-26 2013-12-09 Sugen Inc Aminoheteroarylforbindelser som proteinkinaseinhibitorer
JP4213524B2 (ja) 2003-04-17 2009-01-21 ダイセル化学工業株式会社 新規なカルボニル還元酵素、その酵素をコードするｄｎａを含むポリヌクレオチド、その製造方法、およびこれを利用した光学活性アルコールの製造方法
AU2004266100A1 (en) 2003-08-11 2005-03-03 Codexis, Inc. Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters
DE10345772A1 (de) 2003-10-01 2005-04-21 Basf Ag Verfahren zur Herstellung von 3-Methylamino-1-(thien-2-yl)-propan-1-ol
JP4394647B2 (ja) 2003-12-02 2010-01-06 メルシャン株式会社 光学活性テトラヒドロチオフェン誘導体の製造方法、および、光学活性テトラヒドロチオフェン−３−オールの晶析方法
JP4604524B2 (ja) * 2004-03-16 2011-01-05 味の素株式会社 変異型アルドラーゼ、並びにこれを用いた光学活性ｉｈｏｇ及び光学活性モナティンの製造方法
MX2007001986A (es) 2004-08-26 2007-05-10 Pfizer Compuestos de aminoheteroarilo como inhibidores de proteina quinasa.
CA2577937C (en) 2004-08-26 2010-12-21 Pfizer Inc. Pyrazole-substituted aminoheteroaryl compounds as protein kinase inhibitors
KR20080072105A (ko) 2004-08-26 2008-08-05 화이자 인코포레이티드 단백질 티로신 키나제 저해제 중간체의 제조를 위한거울상이성질선택적 생물학적 전환
JP4242911B2 (ja) 2004-08-26 2009-03-25 ファイザー・インク プロテインキナーゼ阻害薬としてのエナンチオピュアなアミノヘテロアリール化合物
AT501496B1 (de) 2005-02-21 2007-03-15 Iep Gmbh Verfahren zur enantioselektiven enzymatischen reduktion von ketoverbindungen
US7393667B2 (en) 2005-05-31 2008-07-01 Bristol-Myers Squibb Company Stereoselective reduction process for the preparation of pyrrolotriazine compounds
AT502395B1 (de) 2005-07-27 2007-03-15 Iep Gmbh Oxidoreduktasen zur stereoselektiven reduktion von ketoverbindungen
EP2066788B1 (en) 2006-10-02 2014-07-23 Codexis, Inc. Compositions and methods for producing stereoisomerically pure statins and synthetic intermediates therefor
JP2010517574A (ja) 2007-02-08 2010-05-27 コデクシス， インコーポレイテッド ケトレダクターゼおよびその使用
CN101784669B (zh) 2007-08-24 2015-02-18 科德克希思公司 用于(r)-3-羟基四氢噻吩的立体选择性制备的改善的酮还原酶多肽
CN101855342B (zh) 2007-09-13 2013-07-10 科德克希思公司 用于还原苯乙酮的酮还原酶多肽
CN101889081B (zh) 2007-09-28 2014-06-18 科德克希思公司 酮还原酶多肽及其用途
SI2205727T1 (sl) 2007-10-01 2015-09-30 Codexis, Inc. Polipeptidi ketoreduktaze za izdelavo azetidinona
US8288141B2 (en) 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8288131B2 (en) 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides and uses thereof
EP2329014B1 (en) 2008-08-29 2014-10-22 Codexis, Inc. Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017135A1 (en) * 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides

Also Published As

Publication number Publication date
US8748143B2 (en) 2014-06-10
US11479756B2 (en) 2022-10-25
US20130078692A1 (en) 2013-03-28
EP2198018B1 (en) 2013-11-20
JP2016105739A (ja) 2016-06-16
US20180112191A1 (en) 2018-04-26
US20180371428A1 (en) 2018-12-27
KR101586503B1 (ko) 2016-01-18
US20180201909A1 (en) 2018-07-19
US20250368967A1 (en) 2025-12-04
JP5973131B2 (ja) 2016-08-23
US20190144837A1 (en) 2019-05-16
US8852909B2 (en) 2014-10-07
US9528131B2 (en) 2016-12-27
EP2198018A2 (en) 2010-06-23
JP6137758B2 (ja) 2017-05-31
WO2009036404A8 (en) 2010-05-06
US8512973B2 (en) 2013-08-20
US20130344552A1 (en) 2013-12-26
CN101855342A (zh) 2010-10-06
US9951318B1 (en) 2018-04-24
US10927351B2 (en) 2021-02-23
US20090191605A1 (en) 2009-07-30
JP2010538657A (ja) 2010-12-16
US12415990B2 (en) 2025-09-16
WO2009036404A3 (en) 2009-07-16
US10227572B2 (en) 2019-03-12
US20210139861A1 (en) 2021-05-13
US20150017695A1 (en) 2015-01-15
JP2018086028A (ja) 2018-06-07
WO2009036404A2 (en) 2009-03-19
US10100288B2 (en) 2018-10-16
US20170067032A1 (en) 2017-03-09
KR20100061525A (ko) 2010-06-07
US20230257721A1 (en) 2023-08-17
US9873863B2 (en) 2018-01-23
JP2015091269A (ja) 2015-05-14

Similar Documents

Publication Publication Date Title
US12415990B2 (en) Ketoreductase polypeptides for the reduction of acetophenones
US20250346935A1 (en) Ketoreductase polypeptides for the production of (r)-3-hydroxythiolane
CN101889081B (zh) 酮还原酶多肽及其用途
CN102186972A (zh) 用于立体选择性生产(4s)-3-[(5s)-5-(4-氟苯基)-5-羟基戊酰基]-4-苯基-1，3-噁唑烷-2-酮的酮还原酶多肽
CN101883846A (zh) 用于生成氮杂环丁酮的还原酶多肽

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant