CN101812408A - Deodorant yeast for refuse landfill and application technology thereof for refuse deodorization - Google Patents
Deodorant yeast for refuse landfill and application technology thereof for refuse deodorization Download PDFInfo
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- CN101812408A CN101812408A CN 201010124151 CN201010124151A CN101812408A CN 101812408 A CN101812408 A CN 101812408A CN 201010124151 CN201010124151 CN 201010124151 CN 201010124151 A CN201010124151 A CN 201010124151A CN 101812408 A CN101812408 A CN 101812408A
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Abstract
The invention discloses a deodorant yeast for a refuse landfill and an application technology thereof for refuse deodorization. Monascus ankamutant MYM2 with the concentration of over 1.0*107cfu/g is evenly poured and sprayed on the surface of substances to be treated in terms of the principle that 1T treatment amount is poured with 1-15kg of bacteria liquid, and the deodorization effect can be realized within 1-3 days. The fungicide prepared by the invention can effectively deodorize for occasions such as refuse landfill, refuse transfer station, restaurants, toilets and the like in which odor is easy to produce, thus solving or alleviating inconvenience in life, working and learning of surrounding people due to odor.
Description
Technical field
The present invention relates to the microbial deodorant technology, relate in particular to and utilize a kind of refuse landfill deodorizing yeast and rubbish deodorizing use technology thereof.
Background technology
At present, about the patent and the bibliographical information of biological deodorizing more, mainly be divided into two big classes, one class is that the microbial deodorant preparation is combined with equipment: as Chinese patent application number 03109680.8, the applying date is on April 11st, 2003, name is called the patent of " manure fermentation microbiobacterial agent of environment-friendly type and preparation method thereof ", Chinese patent application numbers 200610023848.1, the applying date is on February 13rd, 2006, name is called the patent of " biological deodorizing device " and Chinese patent application numbers 200510086403.3, the applying date is on September 12nd, 2005, name is called the patent of " organic solid castoff compost treatment and biological deodorizing system and working method thereof ", this method main drawback is that equipment has high input, the maintenance cost height is unfavorable for China's distributing refuse landfill use; Another kind of is the mixed preparation of many floras, comprising mould, milk-acid bacteria, yeast, kind of microorganism was formed surplus 10 of actinomycetes and photosynthetic bacteriums etc. belonged to tens, be also referred to as the EM microbial inoculum: as Chinese patent application numbers 200510069515.8, the applying date is on May 12nd, 2005, name is called the patent of " a kind of biological deodorization and purification agent and uses thereof ", Chinese patent application numbers 200510056771.3, the applying date is on March 25th, 2005, name is called the patent of " a kind of novel biological deodorization composition and preparation method " and Chinese patent application number 03109680.8, the applying date is on April 11st, 2003, name is called the patent of " manure fermentation microbiobacterial agent of environment-friendly type and preparation method thereof ", such patent is because flora quantity is many, growth differences is big, cause its production cost height, complicated operation, the quality instability also makes its application be restricted.
Summary of the invention
Technical problem solved by the invention is to provide a kind of refuse landfill deodorizing yeast and rubbish deodorizing use technology thereof, to solve the shortcoming in the above-mentioned background technology.
Technical problem solved by the invention realizes by the following technical solutions:
The present invention utilize Chinese typical culture collection center deposit number for the liquid preparation of CCTCC M 2010051 yeast Monascus anka mutant MYM2 with greater than 1.0 * 10
7The concentration of cfu/g according to the usage quantity that the 1T treatment capacity is thrown in 1-15 kilogram bacterium liquid, is evenly splashed in the handled thing surface, can produce deodorizing effect in 1-3 days.
Saccharomycetic liquid preparation preparation method is as follows described in the present invention: 1, culture of seed liquid: adopt Tryptones 5-15 gram, yeast extract 3-10 gram, potassium primary phosphate 0.1-0.5 gram, sodium-chlor 5-15 gram, water 90-120mL is as the seed liquor substratum, cultivated 25-30 hour in 20-35 ℃ of following shaking table, treat that seed liquor liquid presents blush and gets final product; 2, liquid preparation preparation: adopt potato 10-30 gram, sucrose 1-4 gram, potassium primary phosphate 0.1-0.5 gram, sal epsom 0.05-0.2 gram, water 90-120mL is as fermention medium, under 20-35 ℃,, cultivated 30-40 hour, treat that fermented liquid presents blush according to the inoculum size of 3%-8%, pH reduces to below 3.2, the bacterium liquid that obtains of fermentation, be 2.5-3.0 with the vinegar acid for adjusting pH value after, bottling makes liquid preparation.
Among the present invention, the best preparation scheme of liquid preparation is: adopt Tryptones 10g, yeast extract 5g, potassium primary phosphate 0.2 gram, sodium-chlor 10 grams, water 100mL is as the seed liquor substratum, cultivated 28 hours in 30 ℃ of following shaking tables, treat that seed liquor liquid presents blush; Adopt potato 20g, sucrose 2g, potassium primary phosphate 0.2 gram, sal epsom 0.1 gram, water 100mL is as fermention medium, under 30 ℃,, cultivated 36 hours, treat that fermented liquid presents blush according to 5% inoculum size, pH reduces to below 3.2, the bacterium liquid that obtains of fermentation, be 2.8 with the vinegar acid for adjusting pH value after, bottling makes liquid preparation.
Beneficial effect: the microbial inoculum that adopts the present invention to make can effectively be removed the place that refuse landfill, garbage transfer station, food and drink, lavatory etc. easily produce foul smell, thus solve or alleviate since foul smell to peripheral crowd lives, working and learning bring inconvenience.
Description of drawings
Fig. 1 is an ammonia and concentration of hydrogen sulfide changing trend diagram in time in the specific embodiment 1
Fig. 2 is an ammonia and concentration of hydrogen sulfide changing trend diagram in time in the specific embodiment 2
Embodiment
Give an actual example below and describe the present invention.
Adopt Tryptones 8 grams, yeast extract 6 grams, potassium primary phosphate 0.3 gram, sodium-chlor 8 grams, water 110mL were cultivated 28 hours in 28 ℃ of following shaking tables as the seed liquor substratum, treated that seed liquor liquid presents blush; Adopt potato 25 grams, sucrose 3 grams, potassium primary phosphate 0.3 gram, sal epsom 0.1 gram, water 110mL is as fermention medium, under 30 ℃,, cultivated 38 hours, treat that fermented liquid presents blush according to 7% inoculum size, pH reduces to below 3.2, the bacterium liquid that obtains of fermentation, be 2.8 with the vinegar acid for adjusting pH value after, bottling makes liquid preparation.
The gained liquid preparation is made aqua, make its ultimate density reach 1.0-1.5 * 10
7Cfu/g is used for the refuse landfill of Liuyang City, Hunan Province in May, 2009, according to the 1T rubbish 10 kilograms of bacterium liquid of evenly splashing, detect ammonia and sulfureted hydrogen gas concentration as shown in Figure 1 by the scene, and subjective appreciation is as shown in table 1.
Refuse tip, Liuyang City, table 1 Hunan Province subjective appreciation result
Adopt Tryptones 10g, yeast extract 5g, potassium primary phosphate 0.2 gram, sodium-chlor 10 grams, water 100mL were cultivated 28 hours in 30 ℃ of following shaking tables as the seed liquor substratum, treated that seed liquor liquid presents blush; Adopt potato 20g, sucrose 2g, potassium primary phosphate 0.2 gram, sal epsom 0.1 gram, water 100mL is as fermention medium, under 30 ℃,, cultivated 36 hours, treat that fermented liquid presents blush according to 5% inoculum size, pH reduces to below 3.2, the bacterium liquid that obtains of fermentation, be 2.8 with the vinegar acid for adjusting pH value after, bottling makes liquid preparation.
The gained liquid preparation is made aqua, make its ultimate density reach 1.7-2.0 * 10
7Cfu/g is used for the refuse landfill of Shaoyang, Hunan Province in June, 2009, according to the 1T rubbish 15 kilograms of bacterium liquid of evenly splashing, detect ammonia and sulfureted hydrogen gas concentration as shown in Figure 2 by the scene, and subjective appreciation is as shown in table 2.
Refuse tip, Shaoyang, table 2 Hunan Province subjective appreciation result
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that describes in the foregoing description and the specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (3)
1. refuse landfill deodorizing yeast and rubbish deodorizing use technology thereof is characterized in that, utilize Chinese typical culture collection center deposit number for the liquid preparation of CCTCC M 2010051 yeast Monascus ankamutant MYM2 with greater than 1.0 * 10
7The concentration of cfu/g according to the usage quantity that the 1T treatment capacity is thrown in 1-15 kilogram bacterium liquid, is evenly splashed in the handled thing surface, can produce deodorizing effect in 1-3 days.
2. refuse landfill deodorizing yeast according to claim 1 and rubbish deodorizing use technology thereof, it is characterized in that, the preparation method of described liquid preparation is: 1, culture of seed liquid: adopt Tryptones 5-15 gram, yeast extract 3-10 gram, potassium primary phosphate 0.1-0.5 gram, sodium-chlor 5-15 gram, water 90-120mL is as the seed liquor substratum, cultivated 25-30 hour in 20-35 ℃ of following shaking table, treat that seed liquor liquid presents blush and gets final product; 2, liquid preparation preparation: adopt potato 10-30 gram, sucrose 1-4 gram, potassium primary phosphate 0.1-0.5 gram, sal epsom 0.05-0.2 gram, water 90-120mL is as fermention medium, under 20-35 ℃,, cultivated 30-40 hour, treat that fermented liquid presents blush according to the inoculum size of 3%-8%, pH reduces to below 3.2, the bacterium liquid that obtains of fermentation, be 2.5-3.0 with the vinegar acid for adjusting pH value after, bottling makes liquid preparation.
3. refuse landfill deodorizing yeast according to claim 1 and 2 and rubbish deodorizing use technology thereof, it is characterized in that, the best preparation scheme of described liquid preparation is: adopt Tryptones 10g, yeast extract 5g, potassium primary phosphate 0.2 gram, sodium-chlor 10 grams, water 100mL is as the seed liquor substratum, cultivated 28 hours in 30 ℃ of following shaking tables, treat that seed liquor liquid presents blush; Adopt potato 20g, sucrose 2g, potassium primary phosphate 0.2 gram, sal epsom 0.1 gram, water 100mL is as fermention medium, under 30 ℃,, cultivated 36 hours, treat that fermented liquid presents blush according to 5% inoculum size, pH reduces to below 3.2, the bacterium liquid that obtains of fermentation, be 2.8 with the vinegar acid for adjusting pH value after, bottling makes liquid preparation.
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| CN 201010124151 CN101812408A (en) | 2010-03-15 | 2010-03-15 | Deodorant yeast for refuse landfill and application technology thereof for refuse deodorization |
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| CN 201010124151 CN101812408A (en) | 2010-03-15 | 2010-03-15 | Deodorant yeast for refuse landfill and application technology thereof for refuse deodorization |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102485275A (en) * | 2011-06-22 | 2012-06-06 | 聂莹琨 | Cleanser for decorated indoor air |
| CN103977704A (en) * | 2014-04-29 | 2014-08-13 | 广西北部湾环境科学研究院 | Method for deodorizing garbage by utilizing composite microbial agent |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1445013A (en) * | 2002-03-19 | 2003-10-01 | 广东省微生物研究所 | Biologic deodorization method and its device |
-
2010
- 2010-03-15 CN CN 201010124151 patent/CN101812408A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1445013A (en) * | 2002-03-19 | 2003-10-01 | 广东省微生物研究所 | Biologic deodorization method and its device |
Non-Patent Citations (1)
| Title |
|---|
| 《啤酒科技》 20001231 廖鲜艳，等 啤酒废酵母的研究利用 3－7 1－3 , 第2期 2 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102485275A (en) * | 2011-06-22 | 2012-06-06 | 聂莹琨 | Cleanser for decorated indoor air |
| CN102485275B (en) * | 2011-06-22 | 2015-11-25 | 聂莹琨 | A kind of for the indoor air purification agent after fitting up |
| CN103977704A (en) * | 2014-04-29 | 2014-08-13 | 广西北部湾环境科学研究院 | Method for deodorizing garbage by utilizing composite microbial agent |
| CN103977704B (en) * | 2014-04-29 | 2018-07-17 | 广西鸿生源环保股份有限公司 | A method of deodorization being carried out to rubbish using complex micro organism fungicide |
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Application publication date: 20100825 |