CN101775387B - 一种介孔二氧化钛固定化酶及其制备方法和应用 - Google Patents
一种介孔二氧化钛固定化酶及其制备方法和应用 Download PDFInfo
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- CN101775387B CN101775387B CN2010101307447A CN201010130744A CN101775387B CN 101775387 B CN101775387 B CN 101775387B CN 2010101307447 A CN2010101307447 A CN 2010101307447A CN 201010130744 A CN201010130744 A CN 201010130744A CN 101775387 B CN101775387 B CN 101775387B
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- enzyme
- titanium dioxide
- mesoporous
- immobilized enzyme
- tio
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- 108010093096 Immobilized Enzymes Proteins 0.000 title claims abstract description 73
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 title claims abstract description 58
- 239000004408 titanium dioxide Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 102000004190 Enzymes Human genes 0.000 claims abstract description 77
- 108090000790 Enzymes Proteins 0.000 claims abstract description 77
- 102000006640 gamma-Glutamyltransferase Human genes 0.000 claims abstract description 39
- 101710107035 Gamma-glutamyltranspeptidase Proteins 0.000 claims abstract description 38
- 101710173228 Glutathione hydrolase proenzyme Proteins 0.000 claims abstract description 38
- 238000006243 chemical reaction Methods 0.000 claims abstract description 25
- 229910010413 TiO 2 Inorganic materials 0.000 claims description 54
- 238000013016 damping Methods 0.000 claims description 29
- 239000012530 fluid Substances 0.000 claims description 29
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 20
- 230000003534 oscillatory effect Effects 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 230000003197 catalytic effect Effects 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 3
- 230000006237 glutamylation Effects 0.000 abstract 1
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 30
- 102000018120 Recombinases Human genes 0.000 description 25
- 108010091086 Recombinases Proteins 0.000 description 25
- 239000000243 solution Substances 0.000 description 17
- 238000011084 recovery Methods 0.000 description 14
- 238000005303 weighing Methods 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 239000013335 mesoporous material Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000009413 insulation Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 239000001257 hydrogen Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 230000001131 transforming effect Effects 0.000 description 4
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical group OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000013112 stability test Methods 0.000 description 2
- TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- -1 GGT Proteins 0.000 description 1
- 108020004206 Gamma-glutamyltransferase Proteins 0.000 description 1
- 208000027089 Parkinsonian disease Diseases 0.000 description 1
- 206010034010 Parkinsonism Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 125000002642 gamma-glutamyl group Chemical group 0.000 description 1
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229940002612 prodrug Drugs 0.000 description 1
- 239000000651 prodrug Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
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- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
Claims (9)
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CN2010101307447A CN101775387B (zh) | 2010-03-23 | 2010-03-23 | 一种介孔二氧化钛固定化酶及其制备方法和应用 |
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CN2010101307447A CN101775387B (zh) | 2010-03-23 | 2010-03-23 | 一种介孔二氧化钛固定化酶及其制备方法和应用 |
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CN101775387A CN101775387A (zh) | 2010-07-14 |
CN101775387B true CN101775387B (zh) | 2011-10-19 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102559648B (zh) * | 2012-01-13 | 2014-04-02 | 南京工业大学 | 一种以改性的环氧基树脂为载体的固定化酶及其制备方法和应用 |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102676494B (zh) * | 2012-05-03 | 2013-10-30 | 天津大学 | 核壳结构固定化酶颗粒及其制备方法 |
CN102851272B (zh) * | 2012-09-25 | 2013-10-09 | 南京工业大学 | 一种γ-谷氨酰转肽酶固定化酶及其制备方法和应用 |
CN103937776B (zh) * | 2014-04-10 | 2016-09-14 | 南京工业大学 | 一种以载体两性电解质修饰γ-谷氨酰转肽酶固定化酶的方法 |
CN105565509B (zh) * | 2015-12-23 | 2017-11-21 | 南昌航空大学 | 一种利用TiO2‑邻菲啰啉负载CYP119酶光催化氧化降解甲基橙的方法 |
CN105621627B (zh) * | 2015-12-23 | 2017-12-05 | 南昌航空大学 | 一种利用TiO2‑CeO2负载CYP119酶光催化还原Cr6+的方法 |
CN105754985A (zh) * | 2016-04-15 | 2016-07-13 | 清华大学 | 一种酶-金属离子纳米复合物及其制备方法 |
CN106434581A (zh) * | 2016-09-18 | 2017-02-22 | 南京工业大学 | 一种固定化山梨醇脱氢酶及其固定化方法与应用 |
CN110964712A (zh) * | 2019-12-16 | 2020-04-07 | 牡丹江医学院 | 一种γ-L-谷氨酰-L-半胱氨酸合成用固定化酶的制备方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1526474A (zh) * | 2003-09-22 | 2004-09-08 | 南京工业大学 | 一种高比表面积的氧化钛合成方法 |
CN101648147A (zh) * | 2009-08-28 | 2010-02-17 | 南京工业大学 | 一种介孔复合氧化钛及其制备方法 |
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2010
- 2010-03-23 CN CN2010101307447A patent/CN101775387B/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1526474A (zh) * | 2003-09-22 | 2004-09-08 | 南京工业大学 | 一种高比表面积的氧化钛合成方法 |
CN101648147A (zh) * | 2009-08-28 | 2010-02-17 | 南京工业大学 | 一种介孔复合氧化钛及其制备方法 |
Non-Patent Citations (4)
Title |
---|
Yanjun Jiang et al..The improved stability of enzyme encapsulated in biomimetic titania particles.《Materials Science and Engineering C》.2009,第29卷(第3期),328-334. * |
宋希文等.固定化γ-谷氨酰转肽酶催化合成γ-L-谷氨酰-L-半胱氨酸.《化学反应工程与工艺》.2009,第25卷(第5期),420-425. * |
肖彦羚等.固定化γ-谷氨酰转肽酶的催化特性研究.《现代化工》.2009,第29卷147-149,151. * |
肖海军等.固定化酶及其应用研究进展.《生物学通报》.2001,第36卷(第7期),9-10. * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102559648B (zh) * | 2012-01-13 | 2014-04-02 | 南京工业大学 | 一种以改性的环氧基树脂为载体的固定化酶及其制备方法和应用 |
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Application publication date: 20100714 Assignee: GUANGXI PUBEI PHARMACEUTICAL FACTORY Assignor: Nanjing Tech University Contract record no.: 2015450000042 Denomination of invention: Mesoporous titanium dioxide immobilized enzyme and preparation method and application thereof Granted publication date: 20111019 License type: Exclusive License Record date: 20150708 |
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