CN101760444A - 一种用于绵羊卵母细胞体外的筛选培养方法 - Google Patents
一种用于绵羊卵母细胞体外的筛选培养方法 Download PDFInfo
- Publication number
- CN101760444A CN101760444A CN200910113567A CN200910113567A CN101760444A CN 101760444 A CN101760444 A CN 101760444A CN 200910113567 A CN200910113567 A CN 200910113567A CN 200910113567 A CN200910113567 A CN 200910113567A CN 101760444 A CN101760444 A CN 101760444A
- Authority
- CN
- China
- Prior art keywords
- vitro
- ovocyte
- fluid
- fertilization
- hours
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000000287 oocyte Anatomy 0.000 title claims abstract description 78
- 238000000338 in vitro Methods 0.000 title claims abstract description 37
- 238000012216 screening Methods 0.000 title claims abstract description 34
- 241001494479 Pecora Species 0.000 title claims abstract description 26
- 238000012136 culture method Methods 0.000 title abstract 2
- 230000004720 fertilization Effects 0.000 claims abstract description 28
- 125000000853 cresyl group Chemical group C1(=CC=C(C=C1)C)* 0.000 claims abstract description 26
- 230000035800 maturation Effects 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 239000012530 fluid Substances 0.000 claims abstract description 19
- 238000004043 dyeing Methods 0.000 claims abstract description 14
- 210000001771 cumulus cell Anatomy 0.000 claims abstract description 12
- 210000002966 serum Anatomy 0.000 claims abstract description 12
- 210000000582 semen Anatomy 0.000 claims abstract description 10
- 239000006228 supernatant Substances 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims abstract description 7
- 229930182555 Penicillin Natural products 0.000 claims abstract description 4
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims abstract description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 4
- 210000001672 ovary Anatomy 0.000 claims abstract description 4
- 229940049954 penicillin Drugs 0.000 claims abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 22
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 claims description 16
- 239000009871 tenuigenin Substances 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 14
- 238000010186 staining Methods 0.000 claims description 14
- 210000004681 ovum Anatomy 0.000 claims description 7
- 102000002322 Egg Proteins Human genes 0.000 claims description 6
- 108010000912 Egg Proteins Proteins 0.000 claims description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 6
- 229920000669 heparin Polymers 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 229910052708 sodium Inorganic materials 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims description 4
- 229960001008 heparin sodium Drugs 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims description 3
- 229930182566 Gentamicin Natural products 0.000 claims description 3
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims description 3
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 3
- 229960005309 estradiol Drugs 0.000 claims description 3
- 229930182833 estradiol Natural products 0.000 claims description 3
- 229960002518 gentamicin Drugs 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- NGSFWBMYFKHRBD-UHFFFAOYSA-N sodium;2-hydroxypropanoic acid Chemical compound [Na+].CC(O)C(O)=O NGSFWBMYFKHRBD-UHFFFAOYSA-N 0.000 claims description 3
- QTENRWWVYAAPBI-YCRXJPFRSA-N streptomycin sulfate Chemical compound OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](N=C(N)N)[C@H](O)[C@@H](N=C(N)N)[C@H](O)[C@H]1O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](N=C(N)N)[C@H](O)[C@@H](N=C(N)N)[C@H](O)[C@H]1O QTENRWWVYAAPBI-YCRXJPFRSA-N 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 239000012531 culture fluid Substances 0.000 abstract 2
- 235000013601 eggs Nutrition 0.000 abstract 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 abstract 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 210000000805 cytoplasm Anatomy 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 229960005322 streptomycin Drugs 0.000 abstract 1
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 10
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 10
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 10
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 8
- 230000000694 effects Effects 0.000 description 5
- 241000283690 Bos taurus Species 0.000 description 4
- 229960003180 glutathione Drugs 0.000 description 4
- 235000015424 sodium Nutrition 0.000 description 4
- 241000283707 Capra Species 0.000 description 3
- 235000019687 Lamb Nutrition 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 210000002459 blastocyst Anatomy 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 210000002242 embryoid body Anatomy 0.000 description 2
- 210000002503 granulosa cell Anatomy 0.000 description 2
- 244000309465 heifer Species 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000998584 Nuda Species 0.000 description 1
- LLQCRTZROWMVOL-JISBIHODSA-N Parthenin Chemical compound C[C@H]1CC[C@H]2C(=C)C(=O)O[C@H]2[C@]2(C)C(=O)C=C[C@@]12O LLQCRTZROWMVOL-JISBIHODSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000003570 biosynthesizing effect Effects 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 210000002220 organoid Anatomy 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009101135679A CN101760444B (zh) | 2009-12-08 | 2009-12-08 | 一种用于绵羊卵母细胞体外的筛选培养方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009101135679A CN101760444B (zh) | 2009-12-08 | 2009-12-08 | 一种用于绵羊卵母细胞体外的筛选培养方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101760444A true CN101760444A (zh) | 2010-06-30 |
CN101760444B CN101760444B (zh) | 2012-05-23 |
Family
ID=42491823
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2009101135679A Active CN101760444B (zh) | 2009-12-08 | 2009-12-08 | 一种用于绵羊卵母细胞体外的筛选培养方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101760444B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102344908A (zh) * | 2010-07-29 | 2012-02-08 | 内蒙古大学 | 一种获得去核卵母细胞的方法 |
CN105039245A (zh) * | 2015-07-01 | 2015-11-11 | 浙江大学 | 一种利用3d打印技术促进人未成熟卵母细胞体外成熟的方法 |
CN105255823A (zh) * | 2015-11-30 | 2016-01-20 | 扬州大学 | 一种适用于羔羊卵母细胞体外成熟培养的方法及培养液 |
-
2009
- 2009-12-08 CN CN2009101135679A patent/CN101760444B/zh active Active
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102344908A (zh) * | 2010-07-29 | 2012-02-08 | 内蒙古大学 | 一种获得去核卵母细胞的方法 |
CN105039245A (zh) * | 2015-07-01 | 2015-11-11 | 浙江大学 | 一种利用3d打印技术促进人未成熟卵母细胞体外成熟的方法 |
CN105255823A (zh) * | 2015-11-30 | 2016-01-20 | 扬州大学 | 一种适用于羔羊卵母细胞体外成熟培养的方法及培养液 |
Also Published As
Publication number | Publication date |
---|---|
CN101760444B (zh) | 2012-05-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Wang et al. | Melatonin improves the quality of in vitro produced (IVP) bovine embryos: implications for blastocyst development, cryotolerance, and modifications of relevant gene expression | |
CN102899286B (zh) | C型钠肽在促进牛卵母细胞体外成熟中的应用 | |
CN102140435A (zh) | 一种提高水牛体外胚胎生产效率的方法 | |
CN104087550B (zh) | 一种大鼠心肌细胞的培养方法 | |
CN100334205C (zh) | 一种生产水牛可控性别体外胚胎的方法 | |
CN103184189A (zh) | 一种杂交和牛的培育方法 | |
CN107142239A (zh) | 提高牛体外受精胚胎培养效率的方法 | |
CN105925523A (zh) | 一种赤眼鳍条细胞系及其建立方法与应用 | |
CN108504625A (zh) | 一种小鼠成纤维细胞及其用途 | |
CN101760444B (zh) | 一种用于绵羊卵母细胞体外的筛选培养方法 | |
CN104342400B (zh) | 一种提高绵羊卵母细胞体外发育能力的方法 | |
CN107365738A (zh) | 一种奶牛与黄牛异种体外受精胚胎的制备方法 | |
CN105018418B (zh) | 含Endothelin-1的人类卵母细胞体外成熟培养液和应用 | |
CN107916249A (zh) | 提高牛体细胞克隆胚和体外受精胚的发育质量的培养液和培养方法 | |
Lee et al. | Developmental competence of IVM pig oocytes after SCNT in relation to the shrinkage pattern induced by hyperosmotic treatment | |
CN103013908A (zh) | 牛混合羊精液体外受精新方法 | |
CN113151159B (zh) | 一种卵母细胞体外成熟培养液添加剂及其应用 | |
CN105316282B (zh) | 一种达氏鲟精原细胞培养液及应用 | |
CN101843530A (zh) | 幼畜卵母细胞体外两次受精方法 | |
CN101892194B (zh) | 一种提高绵羊卵母细胞体外利用效率的方法 | |
CN101182489B (zh) | 一种动物体细胞克隆方法 | |
CN111286485B (zh) | 辛酰化Ghrelin在体外抑制卵丘细胞复合体cAMP和MAPK分泌中的应用 | |
Looman et al. | Effects of bovine ova density and culture supplements on cleavage and blastocyst development rates of in vitro embryos | |
CN113249304B (zh) | 萝卜硫素在制备牛卵母细胞体外成熟液中的应用 | |
CN103881967B (zh) | 一种含海藻糖的牛卵母细胞体外成熟培养液及培养方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Chen Dayong Inventor after: Li Junlong Inventor after: He Huijie Inventor after: Tan Heng Inventor after: Ma Yunhui Inventor after: Qiu Chunjuan Inventor after: Wang Liqin Inventor after: Huang Juncheng Inventor before: Huang Juncheng Inventor before: Wang Liqin Inventor before: Zhao Yuncheng Inventor before: Chen Tong Inventor before: Lin Jiapeng |
|
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: No. 468, Ali Mountain street, Urumqi economic and Technological Development Zone, the Xinjiang Uygur Autonomous Region, the Xinjiang Uygur Autonomous Region Patentee after: XINJIANG ACADEMY OF ANIMAL SCIENCES, INSTITUTE OF BIOTECHNOLOGY (CHINA-ASTRALIA SHEEP RESEARCH CENTER OF XINJIANG ACADEMY OF ANIMAL SCIENCES) Address before: 830000 No. 151 East Karamay street, the Xinjiang Uygur Autonomous Region, Urumqi Patentee before: China Australia Sheep Research Center, Animal Science Academy of Xinjiang Uygur Autonomous Region |
|
TR01 | Transfer of patent right |
Effective date of registration: 20180213 Address after: 011800 Wulanchabu City, the Inner Mongolia Autonomous Region, the Inner Mongolia Autonomous Region, the town of Ulan Flower Town, Xiao Da Heihe society Meng mutton science and Technology Park Patentee after: INNER MONGOLIA SAINUO BREEDING SHEEP TECHNOLOGY CO.,LTD. Address before: No. 468, Ali Mountain street, Urumqi economic and Technological Development Zone, the Xinjiang Uygur Autonomous Region, the Xinjiang Uygur Autonomous Region Patentee before: XINJIANG ACADEMY OF ANIMAL SCIENCES, INSTITUTE OF BIOTECHNOLOGY (CHINA-ASTRALIA SHEEP RESEARCH CENTER OF XINJIANG ACADEMY OF ANIMAL SCIENCES) |
|
TR01 | Transfer of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Screening culture method for sheep oocytes in vitro Effective date of registration: 20200417 Granted publication date: 20120523 Pledgee: Wulanchabu Siziwangqi sub branch of Bank of Inner Mongolia Co.,Ltd. Pledgor: INNER MONGOLIA SAINUO BREEDING SHEEP TECHNOLOGY Co.,Ltd. Registration number: Y2020150000012 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20210531 Granted publication date: 20120523 Pledgee: Wulanchabu Siziwangqi sub branch of Bank of Inner Mongolia Co.,Ltd. Pledgor: INNER MONGOLIA SAINUO BREEDING SHEEP TECHNOLOGY Co.,Ltd. Registration number: Y2020150000012 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for screening and culturing ovine oocytes in vitro Effective date of registration: 20210531 Granted publication date: 20120523 Pledgee: Wulanchabu Siziwangqi sub branch of Bank of Inner Mongolia Co.,Ltd. Pledgor: INNER MONGOLIA SAINUO BREEDING SHEEP TECHNOLOGY Co.,Ltd. Registration number: Y2021150000044 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20221213 Granted publication date: 20120523 Pledgee: Wulanchabu Siziwangqi sub branch of Bank of Inner Mongolia Co.,Ltd. Pledgor: INNER MONGOLIA SAINUO BREEDING SHEEP TECHNOLOGY CO.,LTD. Registration number: Y2021150000044 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for screening and culturing sheep oocytes in vitro Effective date of registration: 20221213 Granted publication date: 20120523 Pledgee: Wulanchabu Siziwangqi sub branch of Bank of Inner Mongolia Co.,Ltd. Pledgor: INNER MONGOLIA SAINUO BREEDING SHEEP TECHNOLOGY CO.,LTD. Registration number: Y2022150000110 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |