CN101731665B - Enzymolysis method for crocodile tissues and preparation method for crocodile wine - Google Patents
Enzymolysis method for crocodile tissues and preparation method for crocodile wine Download PDFInfo
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- CN101731665B CN101731665B CN200910213802XA CN200910213802A CN101731665B CN 101731665 B CN101731665 B CN 101731665B CN 200910213802X A CN200910213802X A CN 200910213802XA CN 200910213802 A CN200910213802 A CN 200910213802A CN 101731665 B CN101731665 B CN 101731665B
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Abstract
The invention discloses an enzymolysis method for crocodile tissues and a preparation method for crocodile wine. The enzymolysis method for the crocodile tissues comprises the processes of pretreatment, proteinase enzymolysis, high-temperature inactivation, concentration, paste preparation and the like. The preparation method for the crocodile wine comprises the steps of: mixing crocodile paste obtained by enzymolysis and wine base, storing, filtering and the like. Compared with the traditional method, the method has the advantages that: the prepared crocodile wine contains much more contents of beneficial substances such as amino acid, optimized nucleic acid and the like, and has better health-care effect and auxiliary treatment effect on diseases.
Description
Technical field
The present invention relates to the method for the Crocodile wine of a kind of enzyme solution of crocodile tissues, a kind of material preparation that obtains with the crocodile tissues enzymolysis.
Background technology
Crocodile tissues contains abundant amino acid and optimization nucleic acid to the human body beneficial, and normal food can fill blood, strengthen the bone, drive damp evil, and cough, asthma, rheumatism, anaemia, diabetes, cancer etc. are had auxiliary curative effect preferably.At present, people mainly pass through to cook feed crocodile meat and drink Crocodile wine dual mode, absorb benefit materials such as the interior amino acid of crocodile tissues, optimization nucleic acid, to reach the effect of health care and auxiliary disease treatment.Yet, through the mode of culinary art feed crocodile meat, the amino acid in the crocodile meat with optimize nucleic acid and be difficult for absorbing, only few part can be for the people absorbs, most active ingredient is all failed to absorb and is discharged extremely waste.Through drinking the mode of Crocodile wine, the amino acid of the dissolving in the Crocodile wine, optimization nucleic acid etc. is absorbed preferably, and its health-care effect and auxiliary curative effect are good.Yet, existing Crocodile wine, usually all be through will remove intestines assorted and clean up after crocodile tissues soak (some months even 1-2) for a long time and in the wine base, make, it prepares length consuming time, efficient is low.And soak enzymolysis through the wine base; Its crocodile meat can't be fully by enzymolysis; Various useful amino acid in the crocodile meat, optimization nucleic acid can not be gone out by enzymolysis and get in the wine fully, even soak time is long again, fully enzymolysis of portion of tissue can be arranged all; Its extraction is insufficient, has wasted part crocodile resource.
Summary of the invention
The object of the present invention is to provide a kind of extraction efficiency height and extract sufficient crocodile tissues enzyme solution.
Another object of the present invention is to provide the method for the Crocodile wine of a kind of usefulness material that above-mentioned enzyme solution makes preparation.
A kind of enzyme solution of crocodile tissues comprises the step of following order:
A, preliminary treatment: the crocodile tissues that will treat enzymolysis carries out preliminary treatment, then adds in the enzymolysis cylinder;
B, enzymolysis: in the enzymolysis cylinder, add 3～5 times purified water of crocodile tissues weight, add 1.5%～4.0% protease of crocodile tissues weight, keep temperature to remain on 30～60 ℃, stirred 5～24 hours; Then, add 1.0%～4.0% protease of crocodile tissues weight again, still remain under this temperature conditions, continue to stir 8～24 hours;
C, deactivation: the enzymolysis liquid of step B gained is warming up to 90～100 ℃, kept 30～60 minutes, wherein protease is carried out deactivation;
D, filter out the impurity in the enzymolysis liquid and enzymolysis liquid concentrated.
A kind of preparation method of Crocodile wine comprises the step of following order:
A, the paste crocodile concentrate that said method is made mix with the wine base, and wherein crocodile concentrate and wine base volume ratio are 2～100 ‰;
B, with mixed liquid, under room temperature environment the storage, paste crocodile enzymolysis liquid fully is dissolved in the wine base;
C, be filtered to clarification repeatedly, promptly get.
The enzyme solution of crocodile tissues of the present invention, crocodile tissues are behind enzymolysis, and its active ingredient is able to gone out by enzymolysis and extraction fully, extract fully.And its whole enzymolysis and extraction process only in two or three days can be accomplished, and extraction efficiency is high.
The preparation method of Crocodile wine of the present invention; Its paste extract that directly the crocodile tissues enzymolysis and extraction is come out is soaked in the wine base, mixes with the wine base, through long storage time; Let the wine base under nature; Abundant dissolving paste extract makes in the crocodile cream rich in amino acid and optimizes nucleic acid and be dissolved in the wine base, thereby forms Crocodile wine.Amino acid and the content of optimizing benefit materials such as nucleic acid far surpass tradition and only soak amino acid in the Crocodile wine that crocodile tissues obtains and the content of optimizing benefit materials such as nucleic acid through the wine base in its Crocodile wine, can greatly improve the health-care effect of Crocodile wine and to the adjuvant treatment effect of disease.And the crocodile tissues extract is soaked in the wine base prepares Crocodile wine; It can control amino acid and the content of optimizing nucleic acid in the Crocodile wine very easily; Thereby control the health-care efficacy and the adjuvant treatment effect of Crocodile wine reliably; Also can mix the Crocodile wine of benefit materials content such as different aminoacids and optimization nucleic acid as required, thereby satisfy different needs.
Simultaneously, the prepared Crocodile wine of the present invention also has the effect that improves immunity, establishing-Yang, and the effect with antifatigue of refreshing oneself.
The specific embodiment
A kind of enzyme solution of crocodile meat comprises the step of following order:
A, preliminary treatment: crocodile meat is cleaned, rubbed and be the minced meat shape, add in the enzymolysis cylinder;
B, enzymolysis: the purified water that adds 3～5 times of weight crocodile meat; The trypsase that adds crocodile meat weight 1.5%～4.0% keeps temperature to remain on 40～60 ℃, stirs 5～24 hours; After treating not have minced meat basically; Add the papain of crocodile meat weight 1.5%～4.0%, still remain under this temperature conditions, continue to stir 8～24 hours;
C, deactivation: enzymolysis is warming up to 90～100 ℃ with enzymolysis liquid after finishing, and keeps 30～60 minutes, and wherein each kind of protease is carried out deactivation;
D, to filter out impurity in the enzymolysis liquid, be concentrated into density be the above paste of 1.25g/ml.
In the enzyme solution of above-mentioned crocodile meat; Among the enzymolysis step B, the purified water of adding is the most suitable with 3 times of amounts, and trypsase is the most suitable with 2% usually; For the harder crocodile of older meat; Can suitably trypsase be brought up to 4%, and, can suitably trypsase be reduced to 1.5% for the crocodile more young, that meat is tenderer.
Equally, papain is also suitable with 2%, for the harder crocodile of older meat, can suitably papain be brought up to 4%, and for the crocodile more young, that meat is tenderer, can suitably papain be reduced to 1.5%.
Hydrolysis temperature is the most suitable with 50～52 ℃ among the step B, and hydrolysis result is best.Mixing time behind the adding trypsase was advisable with 7 hours usually, and the mixing time behind the adding papain was advisable with 17 hours.
Used filter among the step D with common filter cloth, gets final product to leach magazine.Concentrate and to adopt common vacuum decompression to concentrate to get final product.
A kind of enzyme solution of crocodile skin comprises the step of following order:
A, preliminary treatment: crocodile skin is cleaned, added in the enzymolysis cylinder, add an amount of purified water, make purified water not have crocodile skin, be heated to 90 ℃, behind the maintenance 30min, stop heating, the water of draining;
B, enzymolysis: the purified water that adds 3～5 times of crocodile skin weight again; Add the bromelain of crocodile skin weight 1.5%～4.0%, temperature remains on 30～50 ℃, stirs 5～24 hours; Basically fully dissolve to crocodile skin; Add the papain of crocodile skin weight 0.5%～2.0% and the bromelain of crocodile skin weight 0.5%～2.0% again, still keep this temperature, stirred 8～24 hours;
C, deactivation: enzymolysis is warming up to 90～100 ℃ with enzymolysis liquid after finishing, and keeps 30～60 minutes, with wherein each kind of protease deactivation;
D, to filter out impurity in the enzymolysis liquid, be concentrated into density be the above paste of 1.25g/ml.
In the enzyme solution of above-mentioned crocodile skin, among the enzymolysis step B, can be after adding purified water, will heat earlier temperature will be risen to 90 ℃, be incubated 2-5h after, temperature is reduced to 30～50 ℃ after, add bromelain again.It can be further well-done with scleroid crocodile skin, thereby make the effect of enzymolysis better, enzymolysis speed is faster.
In order to guarantee the hydrolysis result among the step B, hydrolysis temperature preferably can be controlled in 40～42 ℃ the scope.
Among the enzymolysis step B; The purified water that adds is the most suitable with 3 times of amounts; The bromelain that adds is the most suitable with 2% usually, for the harder crocodile of older meat, can suitably bromelain be brought up to 4%; And, can suitably bromelain be reduced to 1.5% for the crocodile more young, that meat is tenderer.
The papain that adds is the most suitable with 1%, for the harder crocodile of older meat, can suitably papain be brought up to 2%, and for the crocodile more young, that meat is tenderer, can suitably papain be reduced to 0.5%.The bromelain that adds simultaneously with papain; The bromelain that also promptly adds for the second time is the most suitable with 1%; For the harder crocodile of older meat; This time can suitably bromelain be brought up to 2%, and, this time can suitably bromelain be reduced to 0.5% for the crocodile more young, that meat is tenderer.
A kind of preparation method of Crocodile wine comprises the step of following order:
A, the paste crocodile concentrate that enzymolysis is made mix with the wine base, and wherein crocodile concentrate and wine base volume ratio are 2～100 ‰;
B, with mixed liquid, under room temperature environment the storage, paste crocodile enzymolysis liquid fully is dissolved in the wine base;
C, be filtered to clarification repeatedly, promptly get.
Said wine base can adopt liquor, glutinous rice wine or yellow rice wine; The time of the following storage that room temperature is 15～30 ℃ can be 1 month, 2 months, 3 months even longer; Wherein more suitable with 1-3 month, neither need preserve the long time, can guarantee that again paste crocodile enzymolysis liquid is dissolved in the wine base more fully.
A kind of Crocodile wine, its wine base is a liquor, and the ratio of paste crocodile concentrate and wine base is 4～6 ‰, and storage time is at least 1 month under the room temperature; After being filtered to clarification repeatedly, the gained Crocodile wine, its total amino acid content can >=30mg/100ml.
A kind of Crocodile wine, its wine base is a liquor, and the ratio of paste crocodile concentrate and wine base is 6～8 ‰, and storage time is at least 2 months under the room temperature; After being filtered to clarification repeatedly, the gained Crocodile wine, its total amino acid content can >=60mg/100ml.
A kind of Crocodile wine, its wine base is a liquor, and the ratio of paste crocodile concentrate and wine base is 8～10 ‰, and storage time is at least 3 months under the room temperature; After being filtered to clarification repeatedly, the gained Crocodile wine, its total amino acid content can >=80mg/100ml.
A kind of Crocodile wine, its wine base is a glutinous rice wine, and the ratio of paste crocodile concentrate and wine base is 6～10 ‰, and storage time is at least 1 month under the room temperature; After being filtered to clarification repeatedly, the gained Crocodile wine, its total amino acid content can >=80mg/100ml.
A kind of Crocodile wine, its wine base is a yellow rice wine, and the ratio of paste crocodile concentrate and wine base is 6～10 ‰, and storage time is at least 1 month under the room temperature; After being filtered to clarification repeatedly, the gained Crocodile wine, its total amino acid content can >=80mg/100ml.
Show that through experiment the Crocodile wine of method preparation of the present invention also has the effect that improves immunity and establishing-Yang.
Concrete test data is following:
(1), test material and animal used as test:
Crocodile wine: the Crocodile wine of method preparation of the present invention concentrates dosage and supplies examination.
Animal: the WISTER rat that grows up, SPF level.34, male and female 4; 6, body weight 180-220g;
(2), test method:
Divide high dose, low dosage and 3 groups of blank, give every day and feed the experiment Crocodile wine 1 time, 2 weeks of continuous irrigation stomach; Give for last time and feed after back 24 hours, weigh, the male and female mouse is closed in the same cage; Observe and sting, lick and ride the hip number of times mutually, observed 2 as a child, get spleen, thymus gland and sexual organ; And weigh respectively, extract eye socket posterior vein blood simultaneously and carry out SOD vigor growing amount mensuration.
(3) concrete test data tabulation is as follows:
| Group | Animal (only) | Dosage | SOD(U /ml) | Spleen weight | Thymic weight | The property amount of thinking highly of | Behaviouristics | First body weight | The last body weight |
| High dose group | 12 | 4 | 137.4 | 0.46 g | 0.29g | 1.61g | 6 times/h | 197g | 226g |
| Low dose group | 12 | 2 | 151.7 | 0.44 g | 0.40g | 1.11g | 13 times/h | 199g | 228g |
| Control group | 12 | - | 120.5 | 0.39 g | 0.34g | 1.09g | 4 times/h | 197g | 227g |
(4), conclusion:
Last table result shows; Compare with control group, the high and low dose group all can slightly increase the weight of spleen and the sexual organ of rat, and the high dose group spleen weight increases more obvious a little than low dose group; But the high dose group thymic weight descends to some extent; Spleen, the atrophy of thymus gland of pointing out this wine can prevent rat have certain facilitation to the hyperplasia of immunocyte, can improve body's immunity.
The behaviouristics aspect: a little higher than control group of high dose group, and low dose group points out this wine can increase the sexual drive of rat apparently higher than high dose group and control group.
The body weight aspect: low dose group has increase slightly.By the SOD growing amount, high dose group and low dose group all have increase, and the low dose group increase is more obvious, point out this wine that certain anti-aging effects is arranged.
In addition, test shows that also the Crocodile wine of the inventive method preparation has the effect of refreshing oneself with antifatigue.
Concrete test data is following:
(1), material and animal
Tried thing: the Crocodile wine of method preparation of the present invention concentrates dosage and supplies examination.
Animal: 24 of adult kunming mices (SPF level), male and female half and half, body weight 18-22g.
(2), test method:
2 dose groups are established in test, and 12/group, male and female half and half are tried thing group (only at every turn irritating stomach amount 0.9ml second) and blank group (physiological saline).Administration every day 1 time, continuous irrigation fed for 2 weeks, carried out swimming time behind the last administration 1h and measured, and climbing mutually to push away when avoiding mouse swimming influences the result, measures 2 mouse at every turn, write down the time of every mouse entry to death, as the mouse swimming time.
(3), experimental result:
| Group | Animal (only) | Dosage | Swimming time (min) | First body weight | The last body weight |
| Tried the thing group | 12 | 0.9ml | 264 | 19g | 29g |
| Control group | 12 | - | 120 | 19g | 33g |
(4), conclusion:
Table 1 is the result show, tried thing group swimming time and obviously is longer than the control group swimming time, points out this drinking utensils that certain antifatigue effect is arranged.
Claims (10)
1. the enzyme solution of a crocodile meat is characterized in that: the step that comprises following order:
A, preliminary treatment: crocodile meat is cleaned, rubbed and be the minced meat shape, then add in the enzymolysis cylinder;
B, enzymolysis: 3～5 times the purified water that adds crocodile meat weight; Add 1.5%～4.0% trypsase of crocodile meat weight, keep temperature to remain on 40～60 ℃, stirred 5～24 hours; After treating not have minced meat basically; Add 1.5%～4.0% papain of crocodile meat weight, still remain under this temperature conditions, continue to stir 8～24 hours;
C, deactivation:
After enzymolysis finishes, the enzymolysis liquid of step B gained is warming up to 90～100 ℃, kept 30～60 minutes, wherein protease is carried out deactivation;
D, to filter out the impurity in the enzymolysis liquid and enzymolysis liquid is concentrated into density be the paste more than the 1.25g/ml.
2. the enzyme solution of crocodile meat according to claim 1 is characterized in that: the purified water that adds among the enzymolysis step B is 3 times of crocodile meat weight, and trypsase is 2% of crocodile meat weight, and papain also is 2% of a crocodile meat weight.
3. the enzyme solution of crocodile meat according to claim 1 is characterized in that: hydrolysis temperature is the most suitable with 50～52 ℃ among the step B.
4. the enzyme solution of a crocodile skin is characterized in that: the step that comprises following order:
A, preliminary treatment: crocodile skin is cleaned, added in the enzymolysis cylinder, add an amount of purified water, make purified water not have crocodile skin, be heated to 90 ℃, behind the maintenance 30min, stop heating, the water of draining;
B, enzymolysis: 3～5 times the purified water that adds crocodile skin weight; Add 1.5%～4.0% bromelain of crocodile skin weight, temperature remains on 30～50 ℃, stirs 5～24 hours; Treat that crocodile skin dissolves basically fully; Add 0.5%～2.0% bromelain of 0.5%～2.0% papain and the crocodile skin weight of crocodile skin weight again, still keep this temperature, stirred 8～24 hours;
C, deactivation:
After enzymolysis finishes, the enzymolysis liquid of step B gained is warming up to 90～100 ℃, kept 30～60 minutes, wherein protease is carried out deactivation;
D, to filter out the impurity in the enzymolysis liquid and enzymolysis liquid is concentrated into density be the paste more than the 1.25g/ml.
5. crocodile according to claim 4
SkinEnzyme solution, it is characterized in that: among the enzymolysis step B, after adding purified water, will heat earlier temperature will be risen to 90 ℃, be incubated 2-5h after, temperature is reduced to 30～50 ℃ after, add bromelain again.
6. crocodile according to claim 4
SkinEnzyme solution, it is characterized in that: among the enzymolysis step B, the purified water of adding is a crocodile
Skin3 times of weight, the bromelain that adds for the first time is a crocodile
Skin2% of weight, the papain of adding are crocodile
Skin1% of weight, bromelain is a crocodile for the second time
Skin1% of weight.
7. according to the enzyme solution of any described crocodile meat among the claim 1-3, it is characterized in that: to be concentrated into density be the above body of paste of 1.25g/ml to enzymolysis liquid among the step D.
8. according to the enzyme solution of any described crocodile skin among the claim 4-6, it is characterized in that: to be concentrated into density be the above body of paste of 1.25g/ml to enzymolysis liquid among the step D.
9. the preparation method of a Crocodile wine comprises the step of following order:
A, the paste crocodile concentrate that claim 7 or 8 described methods are made mix with the wine base, and wherein crocodile concentrate and wine base volume ratio are 2～100 ‰;
Said wine base is liquor, glutinous rice wine or yellow rice wine;
B, with mixed liquid, under room temperature environment the storage, paste crocodile enzymolysis liquid fully is dissolved in the wine base;
C, be filtered to clarification repeatedly, promptly get.
10. according to claim
9The preparation method of described Crocodile wine is characterized in that: room temperature is 15～30 ℃ among the step B, and storage time was controlled at 1-3 month.
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| CN103540511A (en) * | 2013-11-06 | 2014-01-29 | 广东宏益鳄鱼产业有限公司 | Crocodile palm healthcare wine and preparation method thereof |
| CN103540510A (en) * | 2013-11-06 | 2014-01-29 | 广东宏益鳄鱼产业有限公司 | Crocodile bone and meat healthcare wine and preparation method thereof |
| CN104694345A (en) * | 2013-11-06 | 2015-06-10 | 广东宏益鳄鱼产业有限公司 | Crocodile lung health care wine, and preparation method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1160542A (en) * | 1996-11-11 | 1997-10-01 | 朱圣田 | Liquid crocodile composite and its preparation |
| CN1464045A (en) * | 2002-06-16 | 2003-12-31 | 关令华 | Crocodile wine |
| CN1969687A (en) * | 2006-12-01 | 2007-05-30 | 黄学敏 | Crocodile meat preparation and production process thereof |
-
2009
- 2009-12-14 CN CN200910213802XA patent/CN101731665B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1160542A (en) * | 1996-11-11 | 1997-10-01 | 朱圣田 | Liquid crocodile composite and its preparation |
| CN1464045A (en) * | 2002-06-16 | 2003-12-31 | 关令华 | Crocodile wine |
| CN1969687A (en) * | 2006-12-01 | 2007-05-30 | 黄学敏 | Crocodile meat preparation and production process thereof |
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|---|---|---|---|---|
| CN103525657A (en) * | 2013-11-06 | 2014-01-22 | 广东宏益鳄鱼产业有限公司 | Crocodile blood health wine and preparation method |
| CN103540511A (en) * | 2013-11-06 | 2014-01-29 | 广东宏益鳄鱼产业有限公司 | Crocodile palm healthcare wine and preparation method thereof |
| CN103540510A (en) * | 2013-11-06 | 2014-01-29 | 广东宏益鳄鱼产业有限公司 | Crocodile bone and meat healthcare wine and preparation method thereof |
| CN103540510B (en) * | 2013-11-06 | 2015-04-22 | 广东宏益鳄鱼产业有限公司 | Crocodile bone and meat healthcare wine and preparation method thereof |
| CN103540511B (en) * | 2013-11-06 | 2015-04-22 | 广东宏益鳄鱼产业有限公司 | Crocodile palm healthcare wine and preparation method thereof |
| CN104694345A (en) * | 2013-11-06 | 2015-06-10 | 广东宏益鳄鱼产业有限公司 | Crocodile lung health care wine, and preparation method thereof |
| CN103525657B (en) * | 2013-11-06 | 2015-09-16 | 广东宏益鳄鱼产业有限公司 | Crocodile blood health wine and preparation method thereof |
| CN105296312A (en) * | 2015-12-03 | 2016-02-03 | 蔡建中 | Crocodile bone wine and preparation method thereof |
| CN105362470A (en) * | 2015-12-03 | 2016-03-02 | 蔡建中 | Alligator egg liquor and preparation method thereof |
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