CN102676622B - Andrias davidianus nano peptide and andrias davidianus wine - Google Patents
Andrias davidianus nano peptide and andrias davidianus wine Download PDFInfo
- Publication number
- CN102676622B CN102676622B CN201210150482.XA CN201210150482A CN102676622B CN 102676622 B CN102676622 B CN 102676622B CN 201210150482 A CN201210150482 A CN 201210150482A CN 102676622 B CN102676622 B CN 102676622B
- Authority
- CN
- China
- Prior art keywords
- giant salamander
- wine
- andrias davidianus
- peptide
- nano powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses an andrias davidianus nano peptide which is prepared by taking andrias davidianus meat as a raw material and performing complex enzyme enzymolysis on the raw material and can be easily absorbed and used by human bodies. The andrias davidianus nano peptide is blended with base liquor to prepare an andrias davidianus wine which has specific delicate flavor of andrias davidianus and high protein content (0.2 to 8% by weight), and is rich in nutrition. If the andrias davidianus wine is taken for a long time, the functions of invigorating qi, nourishing blood, reinforcing intelligence and nourishing and strengthening a body can be realized.
Description
Technical field
The present invention relates to a kind of with giant salamander (
andrias davidianus) meat be raw material deep processed product, especially preparation method simple, nutritious, be easy to absorption of human body a kind of Megalobatrachus japonicus daoidianuas(Blanchard) is carried out to enzymolysis and giant salamander wine that the giant salamander nanometer peptide obtaining and the giant salamander nanometer peptide of take are prepared as raw material.
Background technology
Giant salamander is the distinctive rare special product of China; belonging to national two classes watches for animals; according in 04 phase of calendar year 2001 < < Guangxi Agricultural bio-science > > road, refreshing Tiger Standard; giant salamander is sweet, flat, light, tonifying Qi, nourish blood, intelligence development, nourishing, strong.Giant salamander is mainly distributed in the streams of Middle And Upper Reaches of The Yangtze River, Zhujiang River middle and upper reaches and river Han upstream.Since the nineties in last century, Hunan, Hubei, Shaanxi and Guangxi have all been set up a large amount of giant salamanders and have been raised and train breeding enterprise, and a year fecundity has reached 600,000 tails.Along with the development of cultivation scale, the intensive processing utilization of Resources of Megalobatrachus Davidianus becomes a urgent problem that is related to the sustainable development of giant salamander industry.Chinese Patent Application No. 201110259097.4 discloses a kind of manufacture craft of giant salamander health promotion wine, what adopt is the meat of giant salamander, skin, bone, liver, blood, fat etc. are smashed to pieces afterwards and entered glutinous rice, by fermentation, the operation such as squeezing, clarification, essence filter, sterilization makes, preparation method is more loaded down with trivial details.China Patent No. ZL200510136628.5 discloses a kind of salamander health care wine and preparation method, that the herbal medicine such as Giant salamander bone and pseudo-ginseng, Tuber Fleeceflower Root, Semen Platycladi, matrimony vine are formed through soaking in Chinese liquor, although preparation method is relatively simple, in wine, can only contain at most a small amount of giant salamander active substance or nutritive ingredient.
Summary of the invention
The present invention is in order to solve the existing above-mentioned technical problem of prior art, provide a kind of preparation method simple, nutritious, be easy to absorption of human body a kind of Megalobatrachus japonicus daoidianuas(Blanchard) is carried out to enzymolysis and giant salamander wine that the giant salamander nanometer peptide obtaining and the giant salamander nanometer peptide of take are prepared as raw material.
Technical solution of the present invention is: a kind of giant salamander nanometer peptide, is characterized in that preparing as follows:
A. the giant salamander after slaughtering is cleaned, got Megalobatrachus japonicus daoidianuas(Blanchard) lyophilize to moisture and be less than 1%, the Megalobatrachus japonicus daoidianuas(Blanchard) super-fine powder of freeze-drying is broken into 500 ~ 5000 object super-fine powder;
B. giant salamander super-fine powder ball milling is become to the giant salamander nano powder of 50 ~ 200 nm;
C. to the 0.01 ~ 0.1M phosphoric acid buffer and the prozyme that add pH 7 ~ 8 in giant salamander nano powder, enzymolysis 12 ~ 48 h, the mass ratio of described giant salamander nano powder and 0.01 ~ 0.1M phosphoric acid buffer is 1:1 ~ 10, described prozyme addition is 10 ~ 25 unit of activity/giant salamander nano powder (g), and described prozyme is in unit of activity ratio, to be that 3:3:4 ratio is mixed by ocean aspartic protease, papoid, stomach en-;
D. centrifuging and taking supernatant, adopting molecular weight cut-off is that ultra-filtration membrane separator below 4000 Da is separated, gets the liquid through ultra-filtration membrane, and collected liquid, by macroporous adsorbent resin and gac postlyophilization, is made to giant salamander nanometer peptide.
A giant salamander wine prepared by the above-mentioned giant salamander nanometer of use peptide, is characterized in that described giant salamander nanometer peptide 0.1 ~ 10% to join in base wine to adjust and convert in mass ratio, is only filtered to clarification.
The present invention be take Megalobatrachus japonicus daoidianuas(Blanchard) as raw material, forms the giant salamander nanometer peptide that is easily absorbed by the body and utilizes through complex enzyme zymohydrolysis; Giant salamander nanometer peptide and base wine are blent into giant salamander wine, not only have the distinctive delicate flavour of giant salamander and protein content higher (0.2 ~ 8% weight ratio), nutrition is abundanter.Long-term taking, can play tonifying Qi, nourishes blood, intelligence development, nourishing, strong effect.
Embodiment
Embodiment 1:
A. the giant salamander after slaughtering is cleaned, removed skin, fat and internal organ, get Megalobatrachus japonicus daoidianuas(Blanchard) and carry out lyophilize; Megalobatrachus japonicus daoidianuas(Blanchard) after freeze-drying is carried out to micronizing with supper micron mill, obtain 500 object giant salamander super-fine powder;
B. with high-energy ball milling pulverizer, giant salamander super-fine powder is pulverized to 4 ~ 20h, use X-ray detection, obtain the giant salamander nano powder of 50 ~ 200nm;
C. to the 0.01 ~ 0.1M phosphoric acid buffer and the prozyme that add pH 7 ~ 8 in giant salamander nano powder, enzymolysis 12 h, the mass ratio of described giant salamander nano powder and 0.01 ~ 0.1M phosphoric acid buffer is 10, described prozyme addition is 15 unit of activity/giant salamander nano powder (g), and described prozyme is in unit of activity ratio, to be that 3:3:4 ratio is mixed by ocean aspartic protease, papoid, stomach en-;
D. centrifuging and taking supernatant, adopting molecular weight cut-off is that ultra-filtration membrane separator below 4000 Da is separated, gets the liquid through ultra-filtration membrane, by collected liquid by macroporous adsorbent resin (HPD system
row) and gac postlyophilization, make giant salamander nanometer peptide.
By giant salamander nanometer peptide and base wine, be 0.1% to adjust and convert in mass ratio, filter the wine of adjusting after converting, to clarification only.
Embodiment 2:
A. the giant salamander after slaughtering is cleaned, removed skin, fat and internal organ, get Megalobatrachus japonicus daoidianuas(Blanchard) and carry out lyophilize; Megalobatrachus japonicus daoidianuas(Blanchard) after freeze-drying is carried out to micronizing with supper micron mill, obtain 5000 object giant salamander super-fine powder;
B. with high-energy ball milling pulverizer, giant salamander super-fine powder is pulverized to 4 ~ 20h, use X-ray detection, obtain the giant salamander nano powder of 50 ~ 200nm;
C. to the 0.01 ~ 0.1M phosphoric acid buffer and the prozyme that add pH 7 ~ 8 in giant salamander nano powder, enzymolysis 12 h, the mass ratio of described giant salamander nano powder and 0.01 ~ 0.1M phosphoric acid buffer is 1, described prozyme addition is 20 unit of activity/giant salamander nano powder (g), and described prozyme is in unit of activity ratio, to be that 3:3:4 ratio is mixed by ocean aspartic protease, papoid, stomach en-;
D. centrifuging and taking supernatant, adopting molecular weight cut-off is that ultra-filtration membrane separator below 4000 Da is separated, gets the liquid through ultra-filtration membrane, and collected liquid, by macroporous adsorbent resin (HPD series) and gac postlyophilization, is made to giant salamander nanometer peptide.
By giant salamander nanometer peptide and base wine, be 10% to adjust and convert in mass ratio, filter the wine of adjusting after converting, to clarification only.
Embodiment 3:
A. the giant salamander after slaughtering is cleaned, removed skin, fat and internal organ, get Megalobatrachus japonicus daoidianuas(Blanchard) and carry out lyophilize; Megalobatrachus japonicus daoidianuas(Blanchard) after freeze-drying is carried out to micronizing with supper micron mill, obtain 2000 object giant salamander super-fine powder;
B. with high-energy ball milling pulverizer, giant salamander super-fine powder is pulverized to 4 ~ 20h, use X-ray detection, obtain the giant salamander nano powder of 50 ~ 200nm;
C. to the 0.01 ~ 0.1M phosphoric acid buffer and the prozyme that add pH 7 ~ 8 in giant salamander nano powder, enzymolysis 12 h, the mass ratio of described giant salamander nano powder and 0.01 ~ 0.1M phosphoric acid buffer is 5, described prozyme addition is 25 unit of activity/giant salamander nano powder (g), and described prozyme is in unit of activity ratio, to be that 3:3:4 ratio is mixed by ocean aspartic protease, papoid, stomach en-;
D. centrifuging and taking supernatant, adopting molecular weight cut-off is that ultra-filtration membrane separator below 4000 Da is separated, gets the liquid through ultra-filtration membrane, and collected liquid, by macroporous adsorbent resin (HPD series) and gac postlyophilization, is made to giant salamander nanometer peptide.
By giant salamander nanometer peptide and base wine, be 5% to adjust and convert in mass ratio, filter the wine of adjusting after converting, to clarification only.
Claims (2)
1. a giant salamander nanometer peptide, is characterized in that preparing as follows:
A. the giant salamander after slaughtering is cleaned, got Megalobatrachus japonicus daoidianuas(Blanchard) lyophilize to moisture and be less than 1%, the Megalobatrachus japonicus daoidianuas(Blanchard) super-fine powder of freeze-drying is broken into 500 ~ 5000 object super-fine powder;
B. giant salamander super-fine powder ball milling is become to the giant salamander nano powder of 50 ~ 200 nm;
C. to the 0.01 ~ 0.1M phosphoric acid buffer and the prozyme that add pH 7 ~ 8 in giant salamander nano powder, enzymolysis 12 ~ 48 h, the mass ratio of described giant salamander nano powder and 0.01 ~ 0.1M phosphoric acid buffer is 1:1 ~ 10, described prozyme addition is 10 ~ 25 unit of activity/every gram of giant salamander nano powder, and described prozyme is in unit of activity ratio, to be that 3:3:4 ratio is mixed by ocean aspartic protease, papoid, stomach en-;
D. centrifuging and taking supernatant, adopting molecular weight cut-off is that ultra-filtration membrane separator below 4000 Da is separated, collects the liquid that sees through ultra-filtration membrane, by macroporous adsorbent resin and gac postlyophilization, makes giant salamander nanometer peptide.
2. with the giant salamander wine that described in claim 1 prepared by giant salamander nanometer peptide, it is characterized in that described giant salamander nanometer peptide 0.1 ~ 10% to join in base wine and adjust and convert in mass ratio, be only filtered to clarification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210150482.XA CN102676622B (en) | 2012-05-16 | 2012-05-16 | Andrias davidianus nano peptide and andrias davidianus wine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210150482.XA CN102676622B (en) | 2012-05-16 | 2012-05-16 | Andrias davidianus nano peptide and andrias davidianus wine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102676622A CN102676622A (en) | 2012-09-19 |
| CN102676622B true CN102676622B (en) | 2014-03-05 |
Family
ID=46809173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210150482.XA Active CN102676622B (en) | 2012-05-16 | 2012-05-16 | Andrias davidianus nano peptide and andrias davidianus wine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102676622B (en) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271351B (en) * | 2013-04-01 | 2014-08-20 | 张家界金鲵生物工程股份有限公司 | Oral giant salamander liquid for brain strengthening, and preparation method thereof |
| CN103271335B (en) * | 2013-04-01 | 2014-08-06 | 张家界金鲵生物工程股份有限公司 | Andrias davidianus softgel for kidney tonifying, and preparation method thereof |
| CN103493969B (en) * | 2013-07-29 | 2015-12-02 | 四川龙王洞生态农业开发有限公司 | A kind of method of producing giant salamander polypeptide powder |
| CN105039096A (en) * | 2015-07-09 | 2015-11-11 | 重庆肽能酒业有限公司 | Preparation method of polypeptide alcoholic drink |
| CN104982970A (en) * | 2015-08-06 | 2015-10-21 | 重庆馗丰食品有限公司 | Making method and product of pure giant salamander meat meal |
| CN106047618A (en) * | 2016-06-28 | 2016-10-26 | 李杨 | Durian health-care wine with anti-oxidation property and preparation method thereof |
| CN106213127A (en) * | 2016-07-15 | 2016-12-14 | 孙跃 | A kind of alimentation composition improving human body skin moisture |
| CN106010883A (en) * | 2016-08-05 | 2016-10-12 | 四川理工学院 | Kiwi berry health wine and preparation method thereof |
| CN106666484B (en) * | 2016-12-23 | 2019-12-20 | 古鲟（大连）生物科技发展有限公司 | Sturgeon composite powder, sturgeon bone wine and application |
| CN107893014A (en) * | 2018-01-04 | 2018-04-10 | 江西婺源祥鲵生物科技股份有限公司 | A kind of preparation method for drinking giant salamander wine |
| CN109730187A (en) * | 2019-02-15 | 2019-05-10 | 张家界（中国）金驰大鲵生物科技有限公司 | Giant salamander product, preparation method and drink with salubrity tonifying kidney and strengthening yang effect |
| CN110607210A (en) * | 2019-10-21 | 2019-12-24 | 成都高新区九州华昀医疗美容门诊部有限公司 | Giant salamander active peptide red wine |
| CN110628554A (en) * | 2019-11-04 | 2019-12-31 | 茅台学院 | Antioxidant giant salamander peptide sauce-flavor wine and preparation method thereof |
| CN112063481A (en) * | 2020-09-15 | 2020-12-11 | 茅台学院 | Preparation method of giant salamander wine rich in amino acids |
| CN112553033A (en) * | 2020-12-11 | 2021-03-26 | 成都鲵肽生物科技有限公司 | Formula and production process of liver-protecting giant salamander liver peptide wine |
| CN113214943A (en) * | 2021-03-15 | 2021-08-06 | 张家界金驰天问农业科技有限公司 | Giant salamander active peptide Maotai-flavor liquor capable of improving drinking comfort |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824761A (en) * | 2005-12-26 | 2006-08-30 | 王建文 | Salamander health care wine and its preparation method |
| CN101731665A (en) * | 2009-12-14 | 2010-06-16 | 林峰亮 | Enzymolysis method for crocodile tissues and preparation method for crocodile wine |
| CN101812116A (en) * | 2010-03-30 | 2010-08-25 | 张家界金驰大鲵生物科技有限公司 | Giant salamander oligosaccharide peptide, preparation method and application thereof in cosmetics |
| CN101857830A (en) * | 2010-06-13 | 2010-10-13 | 福州乾龙生物科技有限公司 | Method for preparing eel polypeptide wine |
| CN102286341A (en) * | 2011-09-02 | 2011-12-21 | 临安兴农农业开发有限公司 | Process for manufacturing giant salamander health promotion wine |
-
2012
- 2012-05-16 CN CN201210150482.XA patent/CN102676622B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824761A (en) * | 2005-12-26 | 2006-08-30 | 王建文 | Salamander health care wine and its preparation method |
| CN101731665A (en) * | 2009-12-14 | 2010-06-16 | 林峰亮 | Enzymolysis method for crocodile tissues and preparation method for crocodile wine |
| CN101812116A (en) * | 2010-03-30 | 2010-08-25 | 张家界金驰大鲵生物科技有限公司 | Giant salamander oligosaccharide peptide, preparation method and application thereof in cosmetics |
| CN101857830A (en) * | 2010-06-13 | 2010-10-13 | 福州乾龙生物科技有限公司 | Method for preparing eel polypeptide wine |
| CN102286341A (en) * | 2011-09-02 | 2011-12-21 | 临安兴农农业开发有限公司 | Process for manufacturing giant salamander health promotion wine |
Non-Patent Citations (4)
| Title |
|---|
| 大鲵黏液酶解产物的制备及抗疲劳作用研究;李伟 等;《食品工业科技》;20111231;第32卷(第06期);146-148,151 * |
| 李伟 等.大鲵黏液酶解产物的制备及抗疲劳作用研究.《食品工业科技》.2011,第32卷(第06期), |
| 酶法水解大鲵蛋白的工艺研究;马小燕 等;《中国酿造》;20091231(第11期);92-95 * |
| 马小燕 等.酶法水解大鲵蛋白的工艺研究.《中国酿造》.2009,(第11期), |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102676622A (en) | 2012-09-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102676622B (en) | Andrias davidianus nano peptide and andrias davidianus wine | |
| CN101812116B (en) | Giant salamander oligosaccharide peptide, preparation method and application thereof in cosmetics | |
| CN103976428B (en) | A kind of Nakai beverage of heat-clearing and fire-reducing | |
| CN101810354B (en) | Crocodile drinking liquid and production process thereof | |
| CN102172289B (en) | Compound collagen electuary with high absorption and utilization rate and preparation method thereof | |
| CN104770725A (en) | Cistanche salsa biological fermentation ferment and manufacturing method thereof | |
| CN102669669A (en) | Deer spleen extract preparation and preparation method thereof | |
| CN102845632B (en) | Safe environmentally-friendly healthy pig feed additive | |
| CN105754809A (en) | Organic selenium-rich original wine with Tibetan cordyceps and method for industrially producing organic selenium-rich original wine | |
| CN104928140A (en) | Compound fermentation type winter-jujube health care wine and production technology thereof | |
| CN103750094A (en) | Male hachinoko health-care paste and preparation method thereof | |
| CN106036325A (en) | Fructus lycii enzyme and preparation method thereof | |
| CN101816441B (en) | Turtle soup and production method thereof | |
| CN102643327A (en) | Preparation method and application of polypeptide of fermented cucumber seeds | |
| CN103445151B (en) | Cordyceps militaris oral liquid and preparation method thereof | |
| CN102408962A (en) | Micro-fermented citrus limon grape wine and preparation method thereof | |
| CN108285850A (en) | A kind of liver-protecting white spirit and preparation method thereof | |
| CN104522662A (en) | Red rice mycelium compound blood sugar-decreasing functional food and preparation method thereof | |
| CN103830280A (en) | Preparation method of spirulina extract | |
| CN110772456B (en) | Miracle fruit leaf compound and application thereof in anti-melanin formation product | |
| CN103627561B (en) | A kind of health wine and preparation method thereof | |
| CN106937731A (en) | Queen bee nit powder extracts and its preparation method and application | |
| CN106036886A (en) | Gingko enzyme and preparation method thereof | |
| CN104381791B (en) | A kind of instant abalone raspberry particle electuary and preparation method thereof | |
| CN103478843A (en) | Alcohol-removing beverage and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |