CN101857830A - Method for preparing eel polypeptide wine - Google Patents

Method for preparing eel polypeptide wine Download PDF

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Publication number
CN101857830A
CN101857830A CN201010201843A CN201010201843A CN101857830A CN 101857830 A CN101857830 A CN 101857830A CN 201010201843 A CN201010201843 A CN 201010201843A CN 201010201843 A CN201010201843 A CN 201010201843A CN 101857830 A CN101857830 A CN 101857830A
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Prior art keywords
eel
wine
polypeptide
preparation
eel polypeptide
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CN201010201843A
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CN101857830B (en
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苏新龙
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Fujian Qianlong Bioengineering Co ltd
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FUZHOU QIANLONG BIOCHEMICAL SCIENCE TECHNOLOGIES Co Ltd
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Abstract

The invention discloses a method for preparing eel polypeptide wine. The method is characterized by ensuring the characteristics of stable polypeptide content, no precipitates, no repetition, adjustable alcohol content and large suitable population of the wine by a twice-adding method of adding polypeptide dry powder through base wine fermentation and adding polypeptide concentrated solution through blending. By the combined method of modern biological engineering technology and the traditional brewing processes, the eel polypeptide wine prepared by the method has a strong, pure and mild mouthfeel, special eel aroma and taste and golden and gleaming color. Ethanol accelerates polypeptide activities, promotes the fast absorption of nutrition elements of unblended wine and enters an organism for circulation use, so that a peptide wine product has the effects of fast supplying nutrition, removing fatigue, stimulating the circulation of blood, removing dampness, dispelling cold and the like and can fast remove peculiar smell so as to be a natural health-care nutrition drink.

Description

A kind of preparation method of eel polypeptide wine
Technical field
The present invention relates to a kind of preparation method of eel polypeptide wine.
Background technology
Eel still can survive after a year and a half " going on a hunger strike ", and the eel life-span of breed was 50 years.The nutritive value of eel is very high, and being commonly called as " genseng in the water " is soft gold in the water, all is regarded as the good merchantable brand that nourishes, improves looks from ancient times to the present in China and Japan.The Japanese just often eats goluptious roast eel meal to drive away severe cold in winter, keep the fishery products of abundant energy as a kind of quick effect nutrient, present eating method mainly is directly eel to be carried out various simple processing, eel is steamed degrease be put into again and soak into eel wine in the high wine, and also do not developed the new working method of eel at present.We adopt eel to be prepared into fermentation that eel peptide liquid or eel peptide powder carry out five cereals again and collude with old slaking and take off into eel peptide wine
Summary of the invention
The preparation method who the objective of the invention is to overcome the deficiencies in the prior art part and a kind of eel polypeptide wine is provided.The present invention need not also can make the good wine of a kind of new type of health by any herbal medicine, and a kind of eel polypeptide wine is provided.The present invention realizes in the following manner:
(1) preparation eel polypeptide
(1) eel is cleaned with clean-out system, Minced Steak is pressed 1: 2 extraordinarily water, sealing with strand eel meat well then;
(2) sterilization: pass through 0.5-1 minute disinfection by ultraviolet light at normal temperatures, open again with the water pump of crossing through ozonization toward fermentor tank;
(3) enzymatic hydrolysis and fermentation: add phosphoric acid and regulate Ph value 6.8-7.2, neutral papoid 8g/kg, pancreatin 3g/kg, polygalacturonase 2g/kg, 55 ℃-65 ℃ of temperature, enzymolysis time 6-8h;
(4) solid-liquid separation gets supernatant liquor;
(5) enzyme that goes out is handled 85 ℃-95 ℃ of temperature, time 5-10 minute;
(6) skimming treatment adopts centrifugation to isolate fat;
(7) flavor is taken off in the mixing in 1: 2 of activated carbon and diatomite; The molecule diaphragm screen advances the technology ultrafiltration, and molecular weight is reached below 5000 dalton;
(8) concentrate, make eel polypeptide concentration reach 30%-36%, the gained concentrated solution reaches and is the eel polypeptide of required preparation;
(2) basic wine preparation
A selects polished rice, barley, Chinese sorghum, corn, pea, and by weight 1: 1: 1: mixing in 0.8: 0.1 was pulverized;
B adds gross weight 0.6% eel polypeptide powder, adopts fragrant distiller's yeast fermentations such as giving off a strong fragrance, sauce perfume (or spice), delicate fragrance according to demand, according to natural climate condition, and time 8-15 days; Eel polypeptide promotes fermentation as the distiller's yeast substratum, improves liquor output, and eel polypeptide nutrition and eel perfume (or spice) are dissolved in the basic wine;
The C distillation method is produced the above eel polypeptide base of 62%vol wine;
(3) get an amount of basic wine, measure the basic wine ethanol number of degrees and content of peptides, with ethanol 52%vol-62%vol, the standard interpolation eel polypeptide concentrated solution and the water that contain peptide 10% are blent according to product needed; The blended liquor ageing is more than 180 days, 8 ℃-28 ℃ of ageing temperature, relative temperature 45%-75%.
Aging wine after the above-mentioned ageing is adopted the activated carbon mode to filter taste removal, removes residue.
The invention has the advantages that:
The method that the present invention adopts modern biotechnology to combine with traditional wine-making technology, its product mouthfeel is strong pure and mild, the distinctive fragrance flavour of eel, golden yellow color is exquisitely carved.Ethanol quickens polypeptide active, promotes former wine nutrition essence rapid absorption, enters the body recycle, makes the peptide wine product have quick extra-nutrition, Ginseng Extract, effects such as stimulating circulation, dehumidify and dispel cold.Can also eliminate the unusual smell fast, be the natural health nourishing beverage.
Description of drawings
Fig. 1 is the process flow sheet of eel polypeptide method for preparing medicated wine;
Specific embodiment
The specific embodiment of the invention now is described in detail in detail in conjunction with the accompanying drawings, and as shown in Figure 1, the concrete preparation method of the present invention is as follows:
(1) preparation eel polypeptide
(1) eel is cleaned with clean-out system, Minced Steak is pressed 1: 2 extraordinarily water, sealing with strand eel meat well then;
(2) sterilization: the eel meat that strand is good passes through 0.5-1 minute apparatus for ultraviolet disinfection at normal temperatures, opens with the water pump of crossing through ozonization again and just finishes sterilization toward fermentor tank.
(3) enzymatic hydrolysis and fermentation: add phosphoric acid and regulate Ph value 6.8-7.2, neutral papoid 8g/kg, pancreatin 3g/kg, polygalacturonase 2g/kg, 55 ℃-65 ℃ of temperature, enzymolysis time 6-8h;
(4) solid-liquid separation gets supernatant liquor;
(5) enzyme that goes out is handled 85 ℃-95 ℃ of temperature, time 5-10 minute;
(6) skimming treatment adopts centrifugation to isolate fat;
(7) flavor is taken off in the mixing in 1: 2 of activated carbon and diatomite; The molecule diaphragm screen advances the technology ultrafiltration, and molecular weight is reached below 5000 dalton;
(8) vacuum concentration makes eel polypeptide concentration reach 30%-36%, and the gained concentrated solution reaches and is also can get the eel polypeptide powder by spraying drying by the eel polypeptide of required preparation;
According to aqueous enzymatic method hydrolysis and fermentation of the present invention, eel is degraded to the micromolecule polypeptide active substance, extract the eel elite and keep its peculiar fragrance flavour.The composition of extract (eel polypeptide) and graph of molecular weight distribution see Table 1 (distribution of table 1 peptide molecular weight)
Molecular weight ranges Peak area per-cent (%, λ 200nm) Number-average molecular weight Weight-average molecular weight
??>5000 ??1.52 ??8985 ??9975
??5000~3000 ??1.34 ??3621 ??3695
??3000~2000 ??2.93 ??2353 ??2386
Molecular weight ranges Peak area per-cent (%, λ 200nm) Number-average molecular weight Weight-average molecular weight
??2000~1000 ??9.63 ??1346 ??1391
??1000~500 ??16.42 ??662 ??682
??500~100 ??39.68 ??178 ??212
??<100 ??28.48 ??/ ??/
Described preparation method also comprises: the spray-dried one-tenth of the eel polypeptide liquid after will concentrating eel peptide powder is standby: be used for the fermentation of basic wine five cereals.
Base method for preparing medicated wine of the present invention is: (1) selects polished rice, barley, Chinese sorghum, corn, pea, and by weight 1: 1: 1: mixing in 0.8: 0.1 was pulverized;
(2) add gross weight 0.6% eel polypeptide powder, adopt fragrant distiller's yeast fermentations such as giving off a strong fragrance, sauce perfume (or spice), delicate fragrance according to the market requirement, according to natural climate condition, time 8-15 days.Eel polypeptide promotes fermentation as the distiller's yeast substratum, improves liquor output, and eel polypeptide nutrition and eel perfume (or spice) are dissolved in the basic wine;
(3) distillation method is produced the above eel polypeptide base of 62%vol wine.
Described basic wine also can directly adopt various ready-made wine on the market.
The preparation method of preparation eel polypeptide wine of the present invention:
At first get an amount of basic wine, measure the basic wine ethanol number of degrees and content of peptides, with ethanol 52%vol-62%vol, the standard interpolation eel polypeptide concentrated solution and the drinks that contain peptide 10% are blent according to product needed; The blended liquor ageing is more than 180 days.8 ℃-35 ℃ of ageing temperature, relative temperature 45%-75%.
The preparation method of described eel polypeptide wine also comprises: with the aging wine after the above-mentioned ageing adopt activated carbon (diatomite or use membrane filtration) filter taste removal, except that residue.
In sum, the present invention has following advantage compared to existing technology:
The method that the present invention adopts modern biotechnology to combine with traditional wine-making technology, its products taste is strong pure and mild, the peculiar fragrance flavour of eel, golden yellow color is exquisitely carved. Ethanol accelerates polypeptide active, promotes former wine nutrition essence to absorb fast, and it is recycling to enter body, makes the peptide wine product have quick extra-nutrition, eliminates fatigue, effects such as stimulating circulation, dehumidify and dispel cold. Can also eliminate the unusual smell fast, be the natural health nourishing beverage. The present invention adds polypeptide dry powder by basic wine fermentation and blends and again add the concentrated double addition method of polypeptide, guarantee in the wine content of peptides stable, without precipitate, not repeatedly, the adjustable characteristics of alcoholic strength, suitable crowd is extensive. The colleague can also be applicable to other multiple occasion according to the prepared eel polypeptide of this method.

Claims (2)

1. the preparation method of an eel polypeptide wine is characterized in that, comprises the steps:
(1) preparation eel polypeptide
(1) eel is cleaned with clean-out system, Minced Steak is pressed 1: 2 extraordinarily water, sealing with strand eel meat well then;
(2) sterilization: pass through 0.5-1 minute disinfection by ultraviolet light at normal temperatures, open again with the water pump of crossing through ozonization toward fermentor tank;
(3) enzymatic hydrolysis and fermentation: add phosphoric acid and regulate Ph value 6.8-7.2, neutral papoid 8g/kg, pancreatin 3g/kg, polygalacturonase 2g/kg, 55 ℃-65 ℃ of temperature, enzymolysis time 6-8h;
(4) solid-liquid separation gets supernatant liquor;
(5) enzyme that goes out is handled 85 ℃-95 ℃ of temperature, time 5-10 minute;
(6) skimming treatment adopts centrifugation to isolate fat;
(7) flavor is taken off in the mixing in 1: 2 of activated carbon and diatomite; The molecule diaphragm screen advances the technology ultrafiltration, and molecular weight is reached below 5000 dalton;
(8) concentrate, make eel polypeptide concentration reach 30%-36%, the gained concentrated solution is the eel polypeptide of required preparation;
(2) basic wine preparation
A selects polished rice, barley, Chinese sorghum, corn, pea, and by weight 1: 1: 1: mixing in 0.8: 0.1 was pulverized;
B adds gross weight 0.6% eel polypeptide powder, adopts fragrant distiller's yeast fermentations such as giving off a strong fragrance, sauce perfume (or spice), delicate fragrance according to demand, according to natural climate condition, and time 8-15 days; Eel polypeptide promotes fermentation as the wine substratum, improves liquor output, and eel polypeptide nutrition and eel perfume (or spice) are dissolved in the basic wine;
The C distillation method is produced the above eel polypeptide base of 62%vol wine;
(3) get an amount of basic wine, measure the basic wine ethanol number of degrees and content of peptides, with ethanol 52%vol-62%vol, the standard interpolation eel polypeptide concentrated solution and the water that contain peptide 10% are blent according to product needed; The blended liquor ageing is more than 180 days, 8 ℃-28 ℃ of ageing temperature, relative temperature 45%-75%.
2. the preparation method of a kind of eel polypeptide wine according to claim 1 is characterized in that: the aging wine after the above-mentioned ageing is adopted the activated carbon mode to filter taste removal, removes residue.
CN 201010201843 2010-06-13 2010-06-13 Method for preparing eel polypeptide wine Expired - Fee Related CN101857830B (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676622A (en) * 2012-05-16 2012-09-19 张家界(中国)金驰大鲵生物科技有限公司 Andrias davidianus nano peptide and andrias davidianus wine
CN103160420A (en) * 2013-04-03 2013-06-19 陈献君 Crocodile fleece-flower root wine
CN103865737A (en) * 2014-03-19 2014-06-18 武汉鲟龙生物科技有限公司 Method for preparing wine by using sturgeon
CN105039096A (en) * 2015-07-09 2015-11-11 重庆肽能酒业有限公司 Preparation method of polypeptide alcoholic drink
CN106036915A (en) * 2016-05-24 2016-10-26 张雅萍 Special diet composition containing marine active polypeptide as well as preparation method and application of special diet composition
CN106754019A (en) * 2016-12-15 2017-05-31 湖北工业大学 A kind of method of utilization anchovy fermenting and producing alec wine
CN107164443A (en) * 2017-06-05 2017-09-15 深圳知本康业有限公司 A kind of soya bean protein polypeptide and application
CN107200778A (en) * 2017-06-22 2017-09-26 乐清瑞耀食品技术有限公司 The preparation method of conger pile active peptides
CN111849676A (en) * 2020-07-13 2020-10-30 山东青州云门酒业(集团)有限公司 Production method of medium peptide soy sauce wine

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1158892A (en) * 1996-10-22 1997-09-10 葛雷 Peptide-containing protein wine

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1158892A (en) * 1996-10-22 1997-09-10 葛雷 Peptide-containing protein wine

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
陈荔红,郑宝东: "鳗鱼酶法水解蛋白工艺", 《福建农林大学学报(自然科学版)》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676622A (en) * 2012-05-16 2012-09-19 张家界(中国)金驰大鲵生物科技有限公司 Andrias davidianus nano peptide and andrias davidianus wine
CN102676622B (en) * 2012-05-16 2014-03-05 张家界(中国)金驰大鲵生物科技有限公司 Andrias davidianus nano peptide and andrias davidianus wine
CN103160420A (en) * 2013-04-03 2013-06-19 陈献君 Crocodile fleece-flower root wine
CN103865737A (en) * 2014-03-19 2014-06-18 武汉鲟龙生物科技有限公司 Method for preparing wine by using sturgeon
CN103865737B (en) * 2014-03-19 2016-03-23 武汉鲟龙生物科技有限公司 A kind of method adopting sturgeon to prepare wine
CN105039096A (en) * 2015-07-09 2015-11-11 重庆肽能酒业有限公司 Preparation method of polypeptide alcoholic drink
CN106036915A (en) * 2016-05-24 2016-10-26 张雅萍 Special diet composition containing marine active polypeptide as well as preparation method and application of special diet composition
CN106754019A (en) * 2016-12-15 2017-05-31 湖北工业大学 A kind of method of utilization anchovy fermenting and producing alec wine
CN106754019B (en) * 2016-12-15 2020-02-14 湖北工业大学 Method for producing fish sauce wine by using anchovy fermentation
CN107164443A (en) * 2017-06-05 2017-09-15 深圳知本康业有限公司 A kind of soya bean protein polypeptide and application
CN107200778A (en) * 2017-06-22 2017-09-26 乐清瑞耀食品技术有限公司 The preparation method of conger pile active peptides
CN111849676A (en) * 2020-07-13 2020-10-30 山东青州云门酒业(集团)有限公司 Production method of medium peptide soy sauce wine

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