CN1313102C - Health caring capsule of soft-shelled turtle and its preparation method - Google Patents
Health caring capsule of soft-shelled turtle and its preparation method Download PDFInfo
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Abstract
The present invention discloses a soft-shelled turtle health care capsule and a preparation method thereof. The principal raw material of the capsule is soft-shelled turtle powder, and the capsule is compatible with DHA microcapsules, ginkgo biloba extract and zinc lactate. The capsule is prepared by the processing techniques, such as freezing and milling of specific turtles, extraction of ethyl esterification of DHA contained in turtle oil, urea inclusion purification and microencapsulation, mixed preparation of each component in proportion, capsules filling, etc. The product has the advantages of food safety, convenient administration, obvious effect, and double function of enhancing human immunity and improving memory of old people. The present invention has the advantage that the present invention not only opens new exploration and utilization way for soft-shelled turtle production with increasing yield, but also provides an ideal soft-shelled turtle health care capsule product for weak and old people, and has wide application prospects.
Description
Technical field
The invention belongs to health food preparation and manufacturing technology field thereof, especially belonging to a kind of is the health-caring capsule and preparation method thereof of primary raw material with the Trionyx sinensis Wiegmann.
Background technology
Trionyx sinensis Wiegmann, nutritious, food flavor deliciousness, and tool enhancing human activity promote effects such as health, and be always very popular.Carnis Trionycis and extract can improve human immunity, enhance metabolism, and strengthen resistance against diseases, and can improve brain function, memory reinforcing, the decline of control brain function, so concerning weakling and old people, can improve body immunity effectively and prevent senile dementia.Therefore in recent years, breeding soft-shell turtle already develops comparatively fast, the breed scale constantly enlarges, the single production development that had both seriously restricted Trionyx sinensis Wiegmann as the famous dish simple edible, fail again to make full use of the nutritional medicinal value that Trionyx sinensis Wiegmann whole body oil, shell, bone etc. are contained, press for and strengthen the research of carrying out Trionyx sinensis Wiegmann deep processing and comprehensive utilization.The development approach of Trionyx sinensis Wiegmann mainly contains products such as instant food, health nutrient dietetic food, biological engineering deep processing and Trionyx sinensis Wiegmann health food compound preparation.Chinese medicine is the national treasure of China, confirms that according to ancient medicine viewpoint and modern scientific research all there is medical function at each position of Trionyx sinensis Wiegmann health, if can use modern science and technology, develops Trionyx sinensis Wiegmann class health product energetically according to international standard, and prospect will be very wide.Though the home and abroad had asked a collection of patent in the exploitation of Trionyx sinensis Wiegmann in the past, ZL patent No. 91101134.X for example, JP 61-47505 etc. all are to be primary raw material with Trionyx sinensis Wiegmann extracting solution or powder, and the nutrient health that other adds compositions such as Mel, albumen is main; The Trionyx sinensis Wiegmann class development that can see in the market also mainly is to improve the health-related food of immunity, as pure Trionyx sinensis Wiegmann powder, Trionyx sinensis Wiegmann health beverage, turtle wine, chelonia pill etc.; Exist processing superficial, problems such as function singleness.
Summary of the invention
The objective of the invention is at above-mentioned existence deficiency, providing a kind of is primary raw material with the Trionyx sinensis Wiegmann powder, compatibility DHA microcapsule, Folium Ginkgo extract and zinc lactate, have the body immunity of raising and improve the memory dual-use function, taking convenience, obvious results Trionyx sinensis Wiegmann health-caring capsule; Another object of the present invention provides the preparation method of this product.
The present invention is based on following know-why and curative effect performance to the selection of raw material:
Trionyx sinensis Wiegmann has another name called Trionyx sinensis Wiegmann, and is cold in nature, salty in the mouth, and its meat, head, blood, gallbladder, fat, ovum and carapace are all pharmaceutically acceptable.Show according to modern study, contain rich in protein, vitamin, linoleic acid in the Trionyx sinensis Wiegmann, even a large amount of levels that also can not make the body inner cholesterol of taking in raise, and help to prevent and treat arteriosclerosis, heart disease and cerebrovascular accident, have the body immunity of raising, promote the circulation of blood vessel tip, improve the visceral motility ability, enhance metabolism, strengthen effects such as resistance against diseases, skin maintenance and slow down aging.
DHA, it is the contained highly unsaturated fatty acid of Turtle Oil, chemical name is docosahexenoic acid (the following DHA that all abbreviates as), is known as " NAOHUANGJIN " in China, is present in mitochondrion, synaptosome and the microsome of brain cell, relevant closely with the function of these organelles, can improve brain function, memory reinforcing and raising IQ delay cerebral atrophy, repair the impaired of cranial nerve and destroy the decline of control brain function.Majority studies show that with advancing age, neurocyte comes off and increases both at home and abroad, learning capacity descends, and therefore, absorbs a certain amount of DHA and can produce useful influence to mammalian nervous growth and learning behavior, delaying the decline of learning and memory, is a kind of effective measures of prevention alzheimer disease.
Folium Ginkgo extract, its main effective ingredient is flavonoid and lactone, a large amount of bases, home and abroad and clinical research show, these effective ingredient all have tangible nootropic effect to multiple different animals kind, animal model and human body, use extract of ginkgo biloba for treating elderly cerebral dysfunction, brain injury sequela etc. abroad clinically in a large number, evident in efficacy.It accelerates patient's reaction, and memory improves, and symptom takes a turn for the better.Similar test has been done with Folium Ginkgo flavone in succession by China Shanghai College of Traditional Chinese Medicine, Medical University Of Anhui, has also obtained result much at one.
Trace element zinc participates in the activity of human body 100 plurality of enzymes, is one of necessary element of enzymatic activity, and it has important physical function and Nutrition to nucleic acid, protein synthesis, amino acid whose metabolism and a lot of enzyme prime system system.Juvenile camp's zinc deficiency of nourishing one's nature can show as inappetence, short, oligophrenia, and old people's zinc deficiency is easily old and feeble, moves dull etc.Shanghai Disease Prevention and Control Centre's nutrition and food safety section height enclose Wei etc. and carry out studies show that of " zinc being to the influence of the inductive mouse aging learning and memory of D-galactose behavior ", zinc supplement can effectively improve the learning and memory ability drop that zinc deficiency causes, improves and obtains learning and memory and keep memory ability.
The object of the invention realizes by following concrete technical scheme:
A kind of Trionyx sinensis Wiegmann health-caring capsule, the weight portion proportioning of this capsular component and each component is:
Trionyx sinensis Wiegmann powder 10-15
DHA microcapsule 5-10
Folium Ginkgo extract 1-3
Zinc lactate 0.3-0.5
Wherein, the DHA microcapsule contains DHA 〉=10%, and Folium Ginkgo extract contains total flavones 〉=15%, zinc lactate purity 98-103%, and wherein zinc content is 22.65%.
Described Trionyx sinensis Wiegmann health-caring capsule, the weight portion proportioning of the component of its optimization and each component is a Trionyx sinensis Wiegmann powder 12, DHA microcapsule 10, Folium Ginkgo extract 2, zinc lactate 0.4.
A kind of preparation method of Trionyx sinensis Wiegmann health-caring capsule, prepared forms according to the following steps:
(1) preparation of Trionyx sinensis Wiegmann powder: fresh and alive Trionyx sinensis Wiegmann is cleaned, and slaughters, and cuts open the belly, and removes internal organs, be cut into piece in-198 ℃ of low temperature freezing and pulverize after, carry out lyophilization again to moisture≤5%, standby;
(2) DHA esterification extraction: fresh Turtle Oil takes vacuum dehydration to handle, and makes its moisture below 0.1%; With the Turtle Oil of vacuum dehydration and dehydrated alcohol and sodium hydroxide volume ratio 0.5-2Kg: 0.5-1.5L by weight: the 0.01-0.05Kg weighing is got the raw materials ready, earlier the dehydration Turtle Oil is heated to 50 ℃-75 ℃, under agitation slowly add dehydrated alcohol and sodium hydroxide, reaction is 1-4 hour under constant temperature, uses 15% salt water washing then, flush away soap stock and other insoluble matters, obtain the Turtle Oil ethyl ester, put it into-20 ℃ of refrigerator overnight, filter, remove precipitation, be the DHA extract; Judge the second esterification yield with thin layer chromatography in the extraction, determine optimum extraction process.
(3) DHA extract purification: carbamide is dissolved in dehydrated alcohol to saturated solution, in addition DHA extract and dehydrated alcohol were mixed in 1: 1 by volume, two liquid are heated to 70 ℃ respectively, by volume 10-22: 0.8-1.5 slowly joins carbamide-ethanol saturated solution in DHA extract-alcoholic solution, be stirred to the solution clarification under the constant temperature, continue under the room temperature to stir 2-5 hour, gained solution is put into-20 ℃ of refrigerator overnight, crystallisation by cooling, the centrifugal supernatant that gets, add 10% hydrochloric acid to PH2-3, add the suitable quantity of water thorough washing, remove carbamide, separate organic facies, be washed to neutrality, add anhydrous sodium sulfate dehydration drying or vacuum dehydration drying, boil off solvent, mix after above-mentioned clathrate process is operated 1 time or 2 times, get highly unsaturated fatty acid DHA, its purity reaches more than 75%.
(4) preparation of DHA microcapsule: with refining Zein powder is the capsule material, DHA and refining Zein powder by weight 1: 3-5 proportioning, add the refining Zein powder of a small amount of distilled water moistening earlier, add DHA again and, stir, grind by emulsifying agent monoglyceride, sucrose ester or the tween 80 of gross weight 1.5-2.5%,-20 ℃ of refrigerator and cooled are hidden standing over night, spray drying gets the DHA microcapsule after ether is washed, standby;
(5) in the product ratio DHA microcapsule, Folium Ginkgo extract, zinc lactate are joined in the Trionyx sinensis Wiegmann powder mix homogeneously;
(6) capsule is filled, polishing, product inspection, packing: step (5) mixture is poured into the pharmagel hard capsule, the buffing machine polishing, after the assay was approved packing product.
The optimised process of described DHA esterification extraction is that the w/v of Turtle Oil and dehydrated alcohol and sodium hydroxide is 1Kg: 0.75L: 0.03Kg, and reflux, extract, is 2 hours under 65 ℃ of conditions.
The optimum process condition of described DHA extract purification is that carbamide-dehydrated alcohol saturated solution is 15: 1 with DHA extract-ethanol solution ratio, 3 hours response time, enclose number of times 1 time.
Beneficial effect of the present invention, the one, made full use of nutrition medicine resources such as Trionyx sinensis Wiegmann whole body meat, oil, shell, bone, especially the garbage Turtle Oil has higher second esterification yield under the extraction conditions of the best, adopt the urea adduct method purification, its highly unsaturated fatty acid (DHA) purity can reach more than 75%, and the highly unsaturated fatty acid yield can reach 6%; The 2nd, the present invention has rationally added Folium Ginkgo extract and zinc lactate when making full use of the Trionyx sinensis Wiegmann medicine resource, and combination back edible safety has also strengthened animal or human's body immunity and improves the function (seeing the test example) of memory; The 3rd, this law low production cost, simple to operate, and be applicable to production in enormous quantities, both the Trionyx sinensis Wiegmann that increases day by day for output produced and had opened up a new development and utilization measure, conveniently take for a long time for weakling and elderly population again, be easy to absorb, strengthening body immunity and improving memory provides an ideal difunctional health promoting product, has broad application prospects.
The specific embodiment
To be described in further detail preparation of the present invention and animal experiment and human clinical's effect by following examples and test example.
Of the present invention:
Trionyx sinensis Wiegmann and Turtle Oil Hangzhou Zhongde Healthy Food Co., Ltd. Trionyx sinensis Wiegmann field produce;
The two woods prepared slices of Chinese crude drugs factories in Folium Ginkgo extract Ningbo produce and contain total flavones 〉=15%;
The production purity 98-103% of zinc lactate Zhengzhou Rui Pu bio-engineering corporation, wherein zinc content is
22.65％。
Carbamide (urea) Ningbo chemical reagent factory produces content and is not less than 99%.
Reagent such as sodium hydroxide, dehydrated alcohol, anhydrous sodium sulfate are homemade analytical reagent.
Embodiment 1 (Trionyx sinensis Wiegmann health-caring capsule preparation 1)
(1) Trionyx sinensis Wiegmann powder preparation: take by weighing 10 kilograms of fresh and alive Trionyx sinensis Wiegmanns, through cleaning, slaughter, cut open the belly, remove internal organs, it is freezing in-198 ℃ of low temperature to be cut into piece, and pulverizer carries out lyophilization again to moisture≤5% after pulverizing, and is standby;
(2) esterification extraction of DHA: take by weighing 3 kilograms of discarded Turtle Oil from slaughtering the field, take vacuum dehydration to handle, its moisture is controlled at below 0.1%; The Turtle Oil of will dewatering and dehydrated alcohol and sodium hydroxide be volume ratio 1Kg by weight: 0.75L: the 0.03Kg weighing is got the raw materials ready; Earlier the dehydration Turtle Oil is heated to 65 ℃, under agitation slowly add dehydrated alcohol and sodium hydroxide, reaction is 2 hours under constant temperature, use 15% salt water washing then, flush away soap stock and other insoluble matters obtain the Turtle Oil ethyl ester, put it into-20 ℃ of refrigerator overnight, filter, remove precipitation, be the DHA extract;
(3) DHA extract purification: carbamide is dissolved in dehydrated alcohol to saturated solution, in addition DHA extract and dehydrated alcohol were mixed in 1: 1 by volume, two liquid are heated to 70 ℃ respectively, carbamide-dehydrated alcohol saturated solution slowly joined in DHA extract-ethanol solution in by volume 15: 1, be stirred to the solution clarification under the constant temperature, continue under the room temperature to stir 3 hours, gained solution is put into-20 ℃ of refrigerator overnight, crystallisation by cooling, the centrifugal supernatant that gets adds 10% hydrochloric acid to PH2-3, add the suitable quantity of water thorough washing, remove carbamide, separate organic facies, be washed to neutrality, add anhydrous sodium sulfate dehydration drying or vacuum dehydration drying, boil off solvent, promptly get highly unsaturated fatty acid DHA, its purity reaches more than 75%;
(4) preparation of DHA microcapsule: with refining Zein powder is the capsule material, 1: 4 by weight proportioning of DHA and refining Zein powder, add earlier the refining Zein powder of a small amount of distilled water moistening, add DHA again and, stir by the emulsifying agent monoglyceride of gross weight 2%, grind, after-20 ℃ of refrigerator and cooled are hidden standing over night, by pretreatment of raw material 40-60 ℃, air intake 160-180 ℃, air draft 60-80 ℃ spray drying gets the DHA microcapsule after ether is washed;
(5) being mixed of capsule contents: with the made Trionyx sinensis Wiegmann powder of step (1) with through the made DHA microcapsule of step (2), (3), (4) and Folium Ginkgo extract that market is purchased and zinc lactate 12: 10: 2 by weight: 0.4 is mixed;
(6) capsule is filled, polishing, product inspection, packing: at 100,000 grades of capsule workshops of cleanliness factor encapsulating capsule, used capsule must meet the requirement of GB13731 pharmagel hard capsule with each component mixture of step (5), the office work environment uses the dehumidifier dehumidifying, keep relative humidity below 45%, the loading amount minus deviation is controlled at below 9%, and the capsule that installs moves in buffing machine and polishes, be up to the standards, the packing product.
Embodiment 2 (Trionyx sinensis Wiegmann health-caring capsule preparation 2)
In the present embodiment:
DHA esterification extraction process conditions are: the w/v of dehydration Turtle Oil and dehydrated alcohol and sodium hydroxide is 0.6Kg: 1.3L: 0.015Kg, and 50 ℃ of heating-up temperatures were reacted 3 hours;
DHA extract purifying process condition is: carbamide-dehydrated alcohol saturated solution and DHA extract-ethanol solution volume ratio are 11: 1.5, continue under the room temperature to stir enclose number of times 2 times 2 hours;
DHA microcapsule preparation process condition is: 1: 3 by weight proportioning of DHA and refining Zein powder adds the emulsifying agent sucrose ester by gross weight 1.5% again;
The mixed ratio of capsule contents is: Trionyx sinensis Wiegmann powder, DHA microcapsule, Folium Ginkgo extract and zinc lactate weight part ratio are 10: 8: 1.5: 0.3 is mixed.
All the other process of present embodiment all are same as embodiment 1.
Embodiment 3 (Trionyx sinensis Wiegmann health-caring capsule preparation 3)
In the present embodiment:
DHA esterification extraction process conditions are: the w/v 2Kg of dehydration Turtle Oil and dehydrated alcohol and sodium hydroxide: 0.5L: 0.045Kg, and 75 ℃ of heating-up temperatures were reacted 1 hour;
DHA extract purifying process condition is: carbamide-dehydrated alcohol saturated solution and DHA extract-ethanol solution volume ratio are 20: 0.8, continue under the room temperature to stir enclose number of times 1 time 4 hours;
DHA microcapsule preparation process condition is: 1: 5 by weight proportioning of DHA and refining Zein powder adds the emulsifier tween-80 by gross weight 2.5% again;
The mixed ratio of capsule contents is: Trionyx sinensis Wiegmann powder, DHA microcapsule, Folium Ginkgo extract and zinc lactate weight part ratio are 14: 6: 2.5: 0.5 is mixed;
All the other process of present embodiment all are same as embodiment 1.
Test material:
In the soft-shelled turtle capsules Hangzhou Zhongde Healthy Food Co., Ltd. embodiment of the invention 1 product.
Test example 1 (function of enhancing immunity)
Through Nanjing Medical University's health analyzing and testing center testing identity, per os give continuously mice 250mg/kg.bw, 500mg/kg.bw, 1500mg/kg.bw in soft-shelled turtle capsules 1 month, the result shows:
(1) the inductive mouse spleen lymphocyte conversion ratio of cellular immune function: 1500mg/kg.bw group ConA is significantly higher than the 0mg/kg.bw group; 500mg/kg.bw the weightening finish of group mice auricular concha is significantly organized greater than 0mg/kg.bw.
(2) humoral immune function: 500mg/kg.bw, 1500mg/kg.bw group mice HC50 is significantly higher than the 0mg/kg.bw group; 500mg/kg.bw, 1500mg/kg.bw group mice hemolysis plaque digital display work is higher than the 0mg/kg.bw group.
(3) NK cytoactive: 500mg/kg.bw, 1500mg/kg.bw group NK cells in mice activity is significantly higher than the 0mg/kg.bw group.
Therefore think that soft-shelled turtle capsules has the function of enhancing immunity.
Test example 2 (improving the animal memory)
Through nutrition of Chinese Disease Control and Prevention Center and food safety animal test results judge, think that soft-shelled turtle capsules has the animal of improvement memory impairments.Experimental technique and result are as follows:
1. method:
Select 165 of female Kunming mouses in 6 ages in week, body weight 24-28g, adapted to for 1 week after, mice is divided into water maze, diving tower at random, keeps away dark 3 tests, before experiment, animal is carried out primary dcreening operation, water maze test is after training, can not swimming to terminal in 3 minutes, the person eliminates; Diving tower and keep away dark test, animal via 5 minutes training still can not the challenger or electric shock wounded body eliminate, each group keeps 48 of mices altogether, is divided into normal, basic, normal, high dosage group respectively, every group 12, tried thing through irritating stomach, dosage setting is respectively 0.25g/kgbw, 0.50g/kgbw, 0.7kg/kgbw, is dissolved in the deionized water, give sample 30 days continuously, carry out diving tower, water maze test and keep away the training of dark test, carry out test in 24 hours after 24 hours, the test of disappearing after the week.Duration of test is given sample continuously, and repeated experiments is the same.
2. result:
In the water maze test, the required time of reaching home, middle and high dosage group training achievement all significantly is better than matched group (p＜0.05); The mistake sum, 24 hours performance of the test of middle and high dosage group training achievement and high dose group and the achievement that disappears of afterwards testting in 1 week of middle and high dosage group all significantly are better than matched group (p＜0.05).
In the step down test, the number of animals that shocked by electricity, the achievement that disappears of middle and high 1 week of dosage group back test all significantly is better than matched group (p＜0.05); The incubation period of animal, 24 hours performance of the test of high dose group and the achievement that disappears of testting after 1 week all significantly are better than matched group (p＜0.05); The mistake sum, middle and high dosage group training achievement, 24 hours performance of the test and the achievement that disappears of testting after 1 week all significantly are better than matched group (p＜0.05).
Keep away in the dark test, the number of animals that shocked by electricity, middle dosage group training achievement all significantly is better than matched group (p＜0.05); The incubation period of animal, the high dose group training achievement all significantly is better than matched group (p＜0.05); The mistake sum, middle and high dosage group training achievement significantly is better than matched group (p＜0.05).
Test example 3 (human body auxiliary improvement of memory)
Through nutrition of Chinese Disease Control and Prevention Center and food safety the human trial result judge, think that soft-shelled turtle capsules has the effect of auxiliary improvement of memory.Experimental technique and result are as follows:
1. method:
Select the age 111 of the health volunteers in 40-60 year, more than the schooling junior college, do not participate in similar test, do not take before being tried and improve relevant health food of memory function and medicine, through clinical health check-up, no serious cardiovascular disease, serious blood circulation diseases, serious metabolic system disease, serious diseases of urinary system, serious digestive system disease.56 of wherein test-meal groups, 55 of matched groups, matched group is taken the blank placebo of outward appearance unanimity, test-meal group and matched group are oral 2 times of everyone every day, each 2 (2 * 2 * 0.5 gram/people. day), took continuously 45 days, during totally 11 experimenters tried thing or withdraw from test-meal voluntarily and test because of not taking by instructions of taking, have 100 experimenters during off-test and take one's test, each 50 of test-meal group and matched groups.
2. result:
Under each subscale and the isostatic prerequisite of memory quotient of the first two group experimenter of test-meal, through 45 days test-meals test, test-meal group experimenter's sensing memory, image are freed recall and random shape re-recognizes that scale score is significantly higher than matched group (p＜0.05) in the test of subscale.The free recall scale score of subscale of test-meal group experimenter's sensing memory, learning by association, image all has significant rising (p＜0.05) before than test-meal.Image the free recall value added of subscale scale score in test-meal group test back is significantly higher than matched group (p＜0.05).Memory quotient is significantly higher than matched group (p＜0.001) after the preceding experimenter's of test-meal simultaneously the test-meal, and than the rising that highly significant is arranged before the test-meal (p＜0.001), the value added of test-meal group memory quotient is significantly higher than matched group (p＜0.05).According to the criterion in " health food check and assessment technique standard 2003 editions ", think that soft-shelled turtle capsules has the effect of auxiliary improvement of memory.
Test example 4 (toxicity test)
The prepared soft-shelled turtle capsules of the present invention is safe and effective, and taking dose is little.
Detecting unit-Zhejiang Academy of Medical Sciences health food institute toxicologic study through State Food and Drug Administration's assigned food toxicology shows:
1. acute toxicity test
1.1 acute toxicity test in mice: mice is divided into 1.00g/kg, 2.15g/kg, 4.64g/kg, 10.0g/kg four individual weight dosage groups, 10 every group, male and female half and half at random by horn method.Soft-shelled turtle capsules is to male and female its mouse oral LD
50All greater than the 10g/kg body weight, true border is nontoxic.
1.2 rat acute toxicity test: rat is divided into 1.00g/kg, 2.15g/kg, 4.64g/kg, 10.0g/kg four individual weight dosage groups, 10 every group, male and female half and half at random by horn method.Soft-shelled turtle capsules is to male and female rat oral LD
50All greater than the 10g/kg body weight, true border is nontoxic.
2. mouse microkernel test result:
Soft-shelled turtle capsules is to the influence of micronuclei in mice rate: each dosage group of male and female and negative control group relatively, micronucleus permillage there are no significant difference, the positive controls micronuclear rates then is significantly higher than negative control group, its difference has highly significant meaning (P＜0.01).Show that soft-shelled turtle capsules do not see obvious influence to the PCEMNR micronuclear rates, testing result is negative.
3, sperm deformity incidence rate result:
Soft-shelled turtle capsules shows the influence test of mouse sperm deformity incidence rate, each dosage group and negative control group are relatively, mouse sperm deformity incidence rate there are no significant difference, positive controls is then apparently higher than negative control group, its difference has highly significant meaning (P＜0.01), show that soft-shelled turtle capsules do not see obvious influence to the mouse sperm deformity incidence rate, testing result is negative.
4.Ames result of the test:
Variable concentrations is tried thing and is being added and do not adding S
9Under the condition return to become bacterium colony close with negative control group, and positive control returns change bacterium colony number average and is higher than negative control and returns the change clump count more than 2 times.Soft-shelled turtle capsules Salmonella reversion test testing result is negative.
Claims (5)
1, a kind of Trionyx sinensis Wiegmann health-caring capsule is characterized in that the weight portion proportioning of this capsular component and each component is:
Trionyx sinensis Wiegmann powder 10-15
DHA microcapsule 5-10
Folium Ginkgo extract 1-3
Zinc lactate 0.3-0.5.
2, Trionyx sinensis Wiegmann health-caring capsule according to claim 1, the weight portion proportioning that it is characterized in that this capsular component and each component is a Trionyx sinensis Wiegmann powder 12, DHA microcapsule 10, Folium Ginkgo extract 2, zinc lactate 0.4.
3, a kind of method for preparing claim 1 or 2 described Trionyx sinensis Wiegmann health-caring capsules is characterized in that condition is prepared from according to the following steps:
(1) preparation of Trionyx sinensis Wiegmann powder: fresh and alive Trionyx sinensis Wiegmann is cleaned, and slaughters, and cuts open the belly, and removes internal organs, be cut into piece in-198 ℃ of low temperature freezing and pulverize after, carry out lyophilization again to moisture≤5%, standby;
(2) DHA esterification extraction: with the Turtle Oil of vacuum dehydration and dehydrated alcohol and sodium hydroxide volume ratio 0.5-2Kg: 0.5-1.5L by weight: the 0.01-0.05Kg weighing is got the raw materials ready, earlier the dehydration Turtle Oil is heated to 50 ℃-75 ℃, under agitation slowly add dehydrated alcohol and sodium hydroxide, reaction is 1-4 hour under constant temperature, use 15% salt water washing then, obtain the Turtle Oil ethyl ester, put it into-20 ℃ of refrigerator overnight, filter, remove precipitation, be the DHA extract;
(3) DHA extract purification: carbamide is dissolved in dehydrated alcohol to saturated solution, in addition DHA extract and dehydrated alcohol were mixed in 1: 1 by volume, two liquid are heated to 70 ℃ respectively, by volume 10-22: 0.8-1.5 slowly joins carbamide-ethanol saturated solution in DHA extract-alcoholic solution, be stirred to the solution clarification under the constant temperature, continue under the room temperature again to stir 2-5 hour, gained solution is put into-20 ℃ of refrigerator overnight, crystallisation by cooling, the centrifugal supernatant that gets adds 10% hydrochloric acid to PH2-3, add the suitable quantity of water thorough washing, remove carbamide, separate organic facies, be washed to neutrality, add anhydrous sodium sulfate dehydration, boil off solvent, mix after above-mentioned clathrate process is operated 1 time or 2 times, get the pure product of DHA;
(4) preparation of DHA microcapsule: with refining Zein powder is the capsule material, DHA and refining Zein powder by weight 1: 3-5 proportioning, add the refining Zein powder of a small amount of distilled water moistening earlier, add DHA again and, stir, grind by the emulsifying agent of gross weight 1.5-2.5%,-20 ℃ of refrigerator and cooled are hidden standing over night, spray drying gets the DHA microcapsule after ether is washed, standby;
(5) in proportion DHA microcapsule, Folium Ginkgo extract, zinc lactate are joined in the Trionyx sinensis Wiegmann powder mix homogeneously;
(6) capsule is filled, polishing, product inspection, packing: step (5) mixture is poured into the pharmagel hard capsule, buffing machine polishing, packing after the assay was approved.
4, according to the preparation method of the described Trionyx sinensis Wiegmann health-caring capsule of claim 3, the process conditions that it is characterized in that described DHA esterification extraction are that the w/v of Turtle Oil and dehydrated alcohol and sodium hydroxide is 1Kg: 0.75L: 0.03Kg, and reflux, extract, is 2 hours under 65 ℃ of conditions.
5, according to the preparation method of the described Trionyx sinensis Wiegmann health-caring capsule of claim 3, the process conditions that it is characterized in that described DHA extract purification are that the volume ratio of carbamide-dehydrated alcohol saturated solution and DHA extract-ethanol solution is 15: 1,3 hours response time, enclose number of times 1 time.
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| CN102511825B (en) * | 2011-12-22 | 2013-09-11 | 浙江诚达生物科技开发有限公司 | Functional health microcapsule of turtle oil and for blood fat reduction and preparation method thereof |
| CN102919851B (en) * | 2012-10-26 | 2014-04-02 | 中科乐仁（北京）科技发展有限公司 | Health-care food composition for assisting to improve memory and preparation method thereof |
| CN103238840B (en) * | 2013-05-16 | 2014-10-29 | 汤臣倍健股份有限公司 | Ginkgo biloba extract and fish oil soft capsule and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095598A (en) * | 1993-05-24 | 1994-11-30 | 吉林农业大学 | The preparation method of Trionyx sinensis Wiegmann lixiviating solution and the nutritional health food that utilizes the Trionyx sinensis Wiegmann lixiviating solution to produce |
| CN1030033C (en) * | 1991-03-02 | 1995-10-18 | 张建国 | Method for preparing tonic health product from Amyda sinensis |
| CN1224353C (en) * | 2003-01-16 | 2005-10-26 | 吴睿 | Method for preparing biologic health care beverage using soft-shelled turtle |
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2005
- 2005-08-23 CN CNB2005100604479A patent/CN1313102C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030033C (en) * | 1991-03-02 | 1995-10-18 | 张建国 | Method for preparing tonic health product from Amyda sinensis |
| CN1095598A (en) * | 1993-05-24 | 1994-11-30 | 吉林农业大学 | The preparation method of Trionyx sinensis Wiegmann lixiviating solution and the nutritional health food that utilizes the Trionyx sinensis Wiegmann lixiviating solution to produce |
| CN1224353C (en) * | 2003-01-16 | 2005-10-26 | 吴睿 | Method for preparing biologic health care beverage using soft-shelled turtle |
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| Title |
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| 甲鱼油中多不饱和脂肪酸的提取与纯化研究 徐水祥，周淡宜，徐飞鹤等，食品科学，第25卷第11期 2004 * |
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| CN1733024A (en) | 2006-02-15 |
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