CN105441286A - Bee pupa worm health care wine and preparation method thereof - Google Patents
Bee pupa worm health care wine and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a bee pupa worm health care wine, queen bee embryo or drone pupa is taken as an only raw material, low temperature processing technologies such as refrigeration, vacuum degassing, compound enzyme biological enzymolysis, vacuum centrifugation, bio-demulsification, ultrasonic treatment and high voltage pulse electric field treatment are adopted in the whole process, the utilization rate of the queen bee embryo or drone pupa is effectively improved, and loss of nutrition and health care substances is reduced. Ultrasonic waves and peracetic acid are organically combined to accelerate aging of base wine, the base wine is scientifically compounded with queen bee embryo or drone pupa hydrolysate, high voltage pulse aging accelerating is used again, oxidation of peracetic acid to bioactive components of the queen bee embryo hydrolysate is prevented, the aging effect of the queen bee embryo wine is improved, and the taste, the stability and the clarity of the queen bee embryo or drone pupa wine are improved. Finally the queen bee embryo or drone pupa health care wine which is free of worm fishy smell, high in stability, coordinated in taste, mellow and thick, soft, lasting, appropriate is flavor, high in appropriate and nutritive value, good in health care efficacy, clear and transparent is prepared.
Description
Technical field
The present invention relates to the preparation of health promoting wine, be specifically related to a kind of bee pupa worm health promoting wine and preparation method thereof.
Background technology
" queen bee embryo " is also queen bee nit, and in Chinese classical medical literature, claim " honeybee ", be the inevitable outcome in royal jelly and its product process.Worker bee larvae about 1 age in days moves in artificial Beequeen table by beekeeper, and worker bee is used as the continuous feeding Lac regis apis of queen bee, makes its volume in 120 hours increase by thousand times! Royal jelly moistens it and grows up, owing to being in larva state, so intactly remain various nutritive substance, by genetic analysis, queen bee embryo has acted on longevity and the outwardness reason thereof of queen bee, is in embryonism and more completely remains various nutritive substance, and ancient times is imperial palace tribute, its nutrition and pharmaceutical use are all better than " royal jelly ", are the magical energetic nourishing treasures that the Nature vouchsafes the mankind.In China's first pharmacopeia Shennong's Herbal, queen bee embryo is classified as top grade, " taste is put down, the nontoxic qi-restoratives that is slightly cold is won in wound, and clothes of a specified duration make us gloss, and color is not old, beneficial gas of making light of one's life by commiting suicide ".Supplement to the Herbal claims " honeybee cures mainly erysipelas; rubella; stagnant heat in abdomen, sharp stool and urine, breast augmentation juice; strong essence and blood; be full of body fluid ... " " Mingyi Bielu " claims, and " honeybee is made light of one's life by commiting suicide beneficial gas, and control shallow complexion, menses are not smooth, female's soma is puckery, postpartum, after being ill weakness ... " effects such as " soft liver are nourished blood, kidney-tonifying and sperm-producing, happy face moisturizing, detoxification sore treatment, lactogenesis of stimulating the menstrual flow, qi-restoratives enriching yin " that Compendium of Material Medica thinks that " queen bee embryo " has.The Middle nutrition more than 50 that the main component of queen bee embryo has needed by human body to want is plain; rich in protein; 18 seed amino acids; multivitamin; as vitamin B group, particularly the content of vitamins D exceedes 10 times of Oils,glyceridic,cod-liver, goes back nearly 20 kinds of mineral substance and the trace elements such as calcic, zinc, iron, copper, selenium, manganese, magnesium; superoxide-dismutase (SOD), taurine etc.Modern medicine proves, queen bee nit has following effect: 1, improve nervous function: can eliminate insomnia and dreamful sleep, neurasthenia, tired forgetful, the cardio palmus shape of breathing hard of god; 2, endocrine regulation: queen bee embryo is nutritious, female incretion system can be regulated by nourishing the source ovary of women's youth beauty, there is provided sufficient nutrient to ovary, delay the too early atrophy of ovary, safeguard its normal function, ovary is made to reach healthy state, thus the fatigue and weak that solution female endocrine dyscrasia causes, waist soreness, menoxenia, vaginal dryness, many symptoms such as sexual hypofunction; 3, few to man's sperm count, that motility is poor, liquefying time extends patient is significantly improved, and thus can be used for preventing and treating male sterility; 4, can anti-ageing, beauty treatment, beauty treatment, to pachylosis, wrinkle increases, chloasma, complexion are dark and gloomy, alopecia, disconnected sending out have clear improvement; 5, enhancing development: queen bee embryo enhances metabolism, activated cell vigor, improves a poor appetite, and has additional nutrients, and weak to adult human body's void, lean body mass etc. all have great function; 6, abundant vagusstoff, to malnutrition, hypoevolutism, brain healthy, recovery physique, has obvious effect; 7, strengthening immunity: containing abundant natural taurine in queen bee embryo, to lasting adjustment human physiological functions, activated cell vigor, improving immunity of organisms has remarkable efficacy; 8, Digestive tract: containing rich in protein in queen bee embryo, amino acid, vitamins B
1etc. the synthesis that can promote gastric juice and normal secretions, enliven Digestive tract, have remarkable auxiliary therapeutic action to treatment chronic gastritis, gastro-duodenal ulcer.
Drone pupae is the unfertilized egg of honeybee work at drone house property, hatches into larva, enters pupa time, the honeybee body of gathering in this phase is drone pupae, it forms to suck the growth such as royal jelly, honey and bee pollen, and having very high nutritive value, is one urgently whole food leaved for development.According to scientific analysis, in drone pupae, the content of protein is higher than royal jelly, be pollen, more than 2 times of cow's milk, reach 33.4%, wherein, lysine content 4.45mg/100g, be 5 times of egg, isoleucine content is 2.7%, exceedes other food.The content 16.08% of crude fat, wherein indispensable fatty acid content reaches 19.71%, is more than 2 times of pollen, beef, egg more than 3 times, more than 10 times of cow's milk.The content of VITAMIN has exceeded beef, egg, higher than Oils,glyceridic,cod-liver 10 times.In addition, drone pupae is also containing abundant mineral element, and wherein the content of zinc is 2 times of royal jelly, is more than 10 times of pollen; The content of selenium is up to 2.4-2.8 μ g/100g.Visible, drone pupae is a nutrition treasure-house.Drone pupae not only has good nutritive value, and has good pharmaceutical value.As far back as " figure is through book on Chinese herbal medicine ", " Book of Rites. inside then " etc. just have full and accurate record in ancient books, in Shennong's Herbal, " honeybee is young " is considered as top grade, records the drug effect of " the young smell of honeybee is sweet flat, is slightly cold nontoxic, cures mainly headache, and except poison, qi-restoratives is won "; Record in " Mingyi Bielu ", honeybee son can cure mainly erysipelas, rubella, abdomen Nei Liure, sharp stool and urine, go the effect of floating blood, lower milk, has nourishing and therapeutic action to pregnant woman, puerpera.In Asia, also early there are the custom of edible drone pupae in African torrid areas, Australia and some nationalitys of South America.Along with the development of science and technology, the understanding of people to drone pupae has darker understanding.It is found that, drone pupae has many-sided function to living organism, wherein the metabolism of promotion body, grow and energy goes down, muscle power is weak, malnutritive, Paederus fuscipes Curtis is slow, climacterium, comparatively outstanding in prematurely senile rehabilitation, regulating the curative effect also showing its uniqueness in the diseases such as nervus centralis, endocrine regulation, Digestive tract, blood system.
With queen bee embryo or drone pupae for the more of health promoting wine prepared by raw material, have directly by brewed to complete queen bee embryo and base wine, such as: Chinese patent CN102618423A discloses a kind of Fengwang health-care wine, genus field of health care products.This health preserving wine formula is as follows, and rice wine: queen bee nit: ginger: honey: essence=100: 1: 1: 2.5: 0.025, each component is with mass ratio range.This health preserving wine is suitable for grownup and drinks, and has dispeling cold, whets the appetite, beauty treatment, health care's effect such as to relax the muscles and stimulate the blood circulation.Chinese patent CN101899377A discloses a kind of queen bee wine and brewing method thereof.Wherein containing honeybee and white wine, the number of degrees of white wine are more than 50 degree.Described honeybee can be honeybee, preferably drone pupae, can be also honeybee series products, as royal jelly.Wherein the ratio of various composition is honeybee and/or honeybee series products: wine=1: 2 ~ 1: 10, is 1: 3 ~ 1: 7 preferably.Queen bee wine colourity brewed is in the process of the present invention purer, and mouthfeel is better.Chinese patent (ZL93100883.2) discloses a kind of queen cell high-efficient health-care tonic wine, and it is to mix 2.00% (weight) queen bee embryo in 86.00% (weight) wine base and to add 19 kinds of herbal medicine formulated through special process.This drinking utensils is improved body autoimmunization, resist the disease and anti-ageing certain effect of waiting for a long time., taste of traditional Chinese medicine mouthfeel weight too high containing medicinal herb components in this wine, human consumer is difficult to accept, and is unfavorable for applying.Chinese patent CN102787058B discloses a kind of drone pupae wine, and wherein, described drone pupae wine, according to weight parts, is concocted by following composition and formed: rice spirit 65-75; Bee pupa 20-30; Root of Medicinal Indian mulberry 2-6; Herba Cistanches 2-6; Matrimony vine 1-3; Chinese yam 0.5-1.5; Chinese cassia tree 0.5-1.5; Skunk bush 0.5-1.5; South Dodder Seed Chinese Dodder Seed 0.5-1.5; Honey 3-7.Drone pupae wine provided by the present invention take rice spirit as wine base, using edible animals and plants as in fragrant, taste compound, makes alcoholic drink by certain production and processing technology.Utilize the strengthening by means of tonics of drone pupae, the effect of beneficial gas of making light of one's life by commiting suicide, compatibility enrich and benefit essence and blood, the Chinese patent medicine strengthened muscles and bones, add ultrasonic wave and urge old technology, make this wine be the advantage such as dark amber color and luster, limpid transparent, entrance is sweet, sudden and violent not peppery, long times of aftertaste, and effect is remarkable.The utilization rate of active components of above-mentioned disclosed patent queen bee embryo or drone pupae is low, brew time long, bioactive ingredients loss is large, turbid appearance, has precipitation, has whole grain queen bee embryo inside, affect appetite.
Also have queen bee embryo or drone pupae grinding, broken, such as: Chinese patent CN1376774A discloses a kind of queen bee foetus wine and production technique thereof, this wine is after extracting from the queen bee embryo in bee colony and namely freezing with very low temperature is anxious, again through grinding, by queen bee embryo 10-20% (weight) and base wine 80-90% (weight) high efficient mixed evenly after, then configure after ripening and form.The activeconstituents that this bartender deposits queen bee embryo is given prominence to, and strengthening immunity, strong able-bodied body, promotes longevity, Be very effective.Chinese patent CN102776102B discloses a kind of health promoting wine containing bee product, and the weight part of its raw material and raw material is: the white wine that drone pupae is 1.0 ~ 1.5 weight parts, queen bee nit is 2.0 weight parts, royal jelly is 0.02 ~ 0.04 weight part, Radix Panacis Quinquefolii is 0.05 ~ 0.08 weight part and 50 ~ 70 weight parts; The preparation process that should contain the healthcare products of bee product is as follows: 1) this drone pupae pulverizer or pulverizer are smashed; 2) this queen bee nit pulverizer or pulverizer are smashed; 3) this Radix Panacis Quinquefolii pulverizer or pulverizer are smashed; 4) storage tank put into by the drone pupae this smashed, this queen bee nit smashed, this Radix Panacis Quinquefolii smashed and this royal jelly, this white wine is added this storage tank simultaneously, then by the opening sealing of lid of this storage tank, rock every day this storage tank several under, filter after one month, gained clear liquid is the health promoting wine containing bee product of the present invention.Although above-mentioned disclosed patent improves the utilization ratio of queen bee embryo, also shorten old taking time, wine liquid is still muddy, and with precipitation, bioactive ingredients still loses larger.
In order to improve queen bee embryo or drone pupae health care colours of wine clarity, the carrying out had filtration, ultrafiltration, the treatment process such as freezing, such as: Chinese patent CN103710237B discloses a kind of preparation method of bee wine, comprise the following steps: fine powder shape raw material is fully uniformly mixed with the high-quality Luzhou-flavor Tequ wine of 94% ~ 98% (weight) in proportion by bee pollen 1%, royal jelly powder 0.5%, drone pupae powder 0.5%, queen bee embryo powder 0.5%, 5. other 0.2% (weight) respectively, carries out cold soaking and carry.After 15 days ~ 20 days, carry out coarse filtration, essence filter, freezing rear essence filter (or ultrafiltration) respectively, store sth. in a cellar ripening, finished product.Accurate measurement preparation is carried out again after the bee product that the present invention uses filling a prescription carries out micronizing, ensure that qualitative, the fixing quantity to product, evade in traditional method when using fresh goods and must use a large amount of preservation agent, caused the quality to raw material, quantitatively not easily accurately slap.Although above-mentioned disclosed patent clarity increases, but contain significant quantities of fat and protein in its bee product and auxiliary material thereof, be cause basic reason that is muddy and precipitation, in the short period of time, wine liquid can be oxidized by muddiness and produce precipitation, does not fundamentally solve outward appearance clarity problem.
Chinese patent CN103725742A discloses a kind of method that enzymolysis process prepares queen bee embryo peptide, comprise: sample pretreatment, the degreasing of queen bee nit powder, the preparation of queen bee larva sample liquid, ultrasonication queen bee larva sample liquid, queen bee larva sample liquid enzyme digestion reaction, enzymolysis solution is placed in boiling water bath heating 20 minutes enzymes that go out, be cooled to normal temperature, enzymolysis solution pH value is adjusted to 7.0, after centrifugal, gets supernatant liquor, dry above-mentioned supernatant liquor obtains the queen bee embryo Gly-His-Lys be hydrolyzed.The queen bee embryo polypeptide that the present invention obtains can be used in making the healthcare products improving function of human body, and the more important thing is that screening obtains the real effective biologically active polypeptides of human body from the queen bee embryo polypeptide of hydrolysis.According to the treatment process of queen bee embryo in above-mentioned publication, be expected to obtain clarification, transparent queen bee embryo health promoting wine, but queen bee embryo will just can enzymolysis through freeze-drying, overcritical degreasing, operation is numerous and diverse, bioactive ingredients is easily oxidized, degreasing effect is not ideal simultaneously, and introducing extraction organic solvent exists food safety hidden danger.
Chinese patent CN103005466B discloses a kind of application of drone pupae polypeptide extract, comprises described drone pupae polypeptide extract and has the application in the food of Improving memory and/or anti-oxidant function as the food or preparation with Improving memory and/or anti-oxidant function; Described drone pupae polypeptide extract has the application in the healthcare products of Improving memory and/or anti-oxidant function as the healthcare products or preparation with Improving memory and/or anti-oxidant function; Described drone pupae polypeptide extract is preparing the application had in the medicine of Improving memory and/or anti-oxidant function.The function of the obvious Improving memory of this drone pupae polypeptide extract and good antioxygenation, can be used for as or prepare the product with Improving memory and/or anti-oxidant function.Above-mentioned disclosed patent adopts acetone to carry out degreasing, and there is food safety hidden danger, and adopt type of heating before enzymolysis, raw material availability is low, and cost is high.
To sum up, with queen bee embryo or drone pupae be unique raw material prepare a kind of mouthfeel coordinate, mellow, soft, long, local flavor is suitable for, biologically active substance and be of high nutritive value, the queen bee embryo of clear that health-care effect is good or drone pupae health promoting wine have wide market outlook, are also the long-sought of those skilled in the art.
Summary of the invention
Technical problem solved by the invention is the defect overcoming existing queen bee embryo or drone pupae health promoting wine, with queen bee embryo or drone pupae for raw material, first it is freezing, broken, vacuum outgas takes off raw meat, enzymolysis obtains enzymolysis solution, through repeatedly traditional vacuum separation, breakdown of emulsion obtains not fatty and hydrolyzed solution that is albumen, hydrolyzed solution mixes with base wine, ageing, filter a kind of mouthfeel obtaining having original queen bee embryo or drone pupae bioactive ingredients and local flavor to coordinate, mellow, soft, long, local flavor is suitable for, biologically active substance and being of high nutritive value, the bee pupa worm health promoting wine without worm fishy smell of the clear that health-care effect is good.
In order to achieve the above object, the present invention is by the following technical solutions:
A preparation method for bee pupa worm health promoting wine, comprises the following steps:
1) by fresh queen bee embryo or drone pupae and solion in mass ratio 1:0.5-1.5 mix, in-18--22 DEG C of freezing 20-30min, broken, percentage of damage is 98-100%, broken thing particle diameter 200-300 μm, regulate broken thing pH value to be 5-7 with lactic acid, be warming up to 40-50 DEG C, add the prozyme of fresh queen bee embryo or drone pupae quality 0.10-0.18% wherein, mix, in the degassed de-raw meat 10-15min of vacuum tightness-0.07--0.09MPa, leave standstill, continue enzymolysis 10-15min and obtain enzymolysis solution;
Further, described solion is the aqueous solution containing sodium ion 25-30mg/L, zine ion 17-22mg/L, potassium ion 15-20mg/L, calcium ion 12-14mg/L and magnesium ion 9-11mg/L;
Further, described prozyme quality group becomes: flavor protease: aspartic protease: lipase=2-4:1-3:0.3-0.5;
2) enzymolysis solution is put 5000-7000r/min traditional vacuum 8-10min in vacuum centrifuge, be separated from top to bottom and obtain free-fat, milk sap, hydrolyzed solution and residue, be 45-55 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent breakdown of emulsion 20-40min of milk sap quality 0.08-0.12%, in 4000-6000r/min traditional vacuum 2 times after breakdown of emulsion, interval time 1-3h, each centrifugal 10-15min, merge three centrifugal hydrolyzed solutions obtained, get the egg white of its quality 0.2-0.4%, put into stirrer and stir 10-12min in rotating speed 800-1000r/min, until egg white all becomes foam obtain egg white foams, egg white foams is added in the hydrolyzed solution merging and obtain, normal pressure boils 5-8min, be down to room temperature, in 5000-7000r/min traditional vacuum 8-10min, collect hydrolyzed solution, successively through diatomite filtration, namely ultrafiltration obtains transparent, stable queen bee embryo or drone pupae hydrolyzed solution,
Further, described traditional vacuum condition is temperature 6-10 DEG C, vacuum tightness-0.02--0.04MPa;
Further, described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall are in conjunction with the one or more combination in class biological demulsifying agent;
Preferably, the quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=5-7:4-6:2-4;
Preferably, described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl glycoside;
More preferably, the quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl glycoside=3-5:1-2;
3) queen bee embryo or drone pupae hydrolyzed solution quality 1-3 base wine is doubly got, add peracetic acid soln wherein, peroxyacetic acid concentration is made to be 400-480ppm, Homogeneous phase mixing, carries out ultrasonication 5-10min under power 200-400W, frequency 30-40KHz condition, treated base wine and queen bee embryo or drone pupae hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 25-35 DEG C, then by the process of continuous high voltage pulse treatment chamber, leaves standstill, filters and obtain queen bee embryo or drone pupae health promoting wine;
Further, described base wine is 40-60 degree white wine;
Further, the mass percent concentration of described peracetic acid soln is 10-30%;
Further, described pulsed electric field treatment condition are strength of electric field 25-30kV/cm, umber of pulse 40-50.
Another object of the present invention is to provide the queen bee embryo or drone pupae health promoting wine prepared through aforesaid method.
Beneficial effect:
The present invention is unique raw material with queen bee embryo or drone pupae, the low temperature processing techniques such as omnidistance employing is freezing, vacuum outgas, prozyme biological enzymolysis, traditional vacuum, biological demulsifying, supersound process, pulsed electric field process, effectively improve the utilization ratio of queen bee embryo or drone pupae, reduce the loss of nutrition and health substance, the bioactive ingredients remaining queen bee embryo to greatest extent becomes substances content with nutrition.Vacuum outgas and prozyme biological enzymolysis are organically combined, not only reduce the dissolved oxygen content of enzymolysis solution, effectively prevent bioactive ingredients deterioration by oxidation, and eliminate the worm fishy smell of queen bee nit or drone pupae in enzymolysis solution, improve the mouthfeel of health promoting wine, add appetite, the science of solion and queen bee embryo and prozyme is composite, prozyme vigor is played maximum, catalytic capability is more thorough, and then the protein in queen bee embryo and fat are fully degraded, improve stability and the clarity of queen bee foetus wine, wherein generation lipid acid and positively charged ion also significantly can promote the Chen Hua of health promoting wine, biological demulsifying agent breakdown of emulsion and traditional vacuum are organically combined, the free-fat in enzymolysis solution can be made fully to be separated with hydrolyzed solution, obtain nutrition and bioactive substance content enriches, the hydrolyzed solution of clear, add egg white foams add and boil the enzyme that goes out, queen bee embryo or drone pupae hydrolyzed solution are more stable, transparent, by ultrasonic wave and urge old auxiliary agent (Peracetic Acid) organically combine urge Chen Ji wine, with queen bee embryo or drone pupae hydrolyzed solution science composite, high-voltage pulse is again adopted to urge old, both prevented Peracetic Acid to the oxidation of queen bee embryo hydrolyzed solution bioactive ingredients, turn improve the Chen Hua effect of queen bee foetus wine, improve the mouthfeel of queen bee embryo or drone pupae wine, stability and clarity.Worked in coordination with common by the orderly combination of above-mentioned every technical scheme, final obtained a kind of, mouthfeel coordination strong without worm fishy smell, stability, mellow, soft, long, local flavor is suitable for, biologically active substance and be of high nutritive value, the queen bee embryo of clear that health-care effect is good or drone pupae health promoting wine.Concrete test effect is shown in embodiment six to seven.
It should be noted that the health-care effect of queen bee embryo of the present invention or drone pupae health promoting wine is each step technique feature collaborative, interactional result mutually, not simple step continuity and technical characteristic superposition, the combination of each step technique feature and the collaborative effect produced, considerably beyond the superposition of each monotechnics feature functionality and effect, have advanced and practicality preferably.
Embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technique means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Embodiment one:
A kind of queen bee embryo health promoting wine, its preparation method comprises the following steps:
1) by fresh queen bee embryo and solion in mass ratio 1:1 mix, in-20 DEG C of freezing 25min, broken, percentage of damage is 99%, broken thing particle diameter 250 μm, regulate broken thing pH value to be 6 with lactic acid, be warming up to 45 DEG C, add the prozyme of fresh queen bee embryo quality 0.14% wherein, mix, in the degassed de-raw meat 13min of vacuum tightness-0.08MPa, leave standstill, continue enzymolysis 13min and obtain enzymolysis solution;
Above-mentioned solion is the aqueous solution containing sodium ion 28mg/L, zine ion 20mg/L, potassium ion 18mg/L, calcium ion 13mg/L and magnesium ion 10mg/L;
Above-mentioned prozyme quality group becomes: flavor protease: aspartic protease: lipase=3:2:0.4;
2) enzymolysis solution is put in vacuum centrifuge, 6000r/min traditional vacuum 9min, be separated from top to bottom and obtain free-fat, milk sap, hydrolyzed solution and residue, it is 50 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent breakdown of emulsion 30min of milk sap quality 0.10%, in 5000r/min traditional vacuum 2 times after breakdown of emulsion, interval time 2h, each centrifugal 13min, merge three centrifugal hydrolyzed solutions obtained, get the egg white of its quality 0.3%, put into stirrer and stir 11min in rotating speed 900r/min, until egg white all becomes foam obtain egg white foams, egg white foams is added in the hydrolyzed solution merging and obtain, normal pressure boils 7min, be down to room temperature, in 6000r/min traditional vacuum 9min, collect hydrolyzed solution, successively through diatomite filtration, namely ultrafiltration obtains transparent, stable queen bee embryo hydrolyzed solution,
Above-mentioned traditional vacuum condition is temperature 8 DEG C, vacuum tightness-0.03MPa;
The quality group of above-mentioned biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=6:5:3
Above-mentioned glycolipid class biological demulsifying agent quality group becomes: rhamnolipid: alkyl glycoside=4:1.5;
3) the base wine of queen bee embryo hydrolyzed solution quality 2 times is got, add peracetic acid soln wherein, peroxyacetic acid concentration is made to be 440ppm, Homogeneous phase mixing, carries out ultrasonication 8min under power 300W, frequency 35KHz condition, treated base wine and queen bee embryo hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 30 DEG C, then by the process of continuous high voltage pulse treatment chamber, leaves standstill, filters and obtain queen bee embryo health promoting wine;
Above-mentioned base wine is 50 degree of white wine;
The mass percent concentration of above-mentioned peracetic acid soln is 20%;
Above-mentioned pulsed electric field treatment condition are strength of electric field 28kV/cm, umber of pulse 45.
Embodiment two:
A kind of queen bee embryo health promoting wine, its preparation method comprises the following steps:
1) by fresh queen bee embryo and solion in mass ratio 1:0.5 mix, in-18 DEG C of freezing 20min, broken, percentage of damage is 98%, broken thing particle diameter 200 μm, regulate broken thing pH value to be 5 with lactic acid, be warming up to 40 DEG C, add the prozyme of fresh queen bee embryo quality 0.10% wherein, mix, in the degassed de-raw meat 10min of vacuum tightness-0.07MPa, leave standstill, continue enzymolysis 10min and obtain enzymolysis solution;
Above-mentioned solion is the aqueous solution containing sodium ion 25mg/L, zine ion 17mg/L, potassium ion 15mg/L, calcium ion 12mg/L and magnesium ion 9mg/L;
Above-mentioned prozyme quality group becomes: flavor protease: aspartic protease: lipase=2:1:0.3;
2) enzymolysis solution is put 5000r/min traditional vacuum 8min in vacuum centrifuge, be separated from top to bottom and obtain free-fat, milk sap, hydrolyzed solution and residue, it is 45 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent breakdown of emulsion 20min of milk sap quality 0.08%, in 4000r/min traditional vacuum 2 times after breakdown of emulsion, interval time 1h, each centrifugal 10min, merge three centrifugal hydrolyzed solutions obtained, get the egg white of its quality 0.2%, put into stirrer and stir 10min in rotating speed 800r/min, until egg white all becomes foam obtain egg white foams, egg white foams is added in the hydrolyzed solution merging and obtain, normal pressure boils 5min, be down to room temperature, in 5000r/min traditional vacuum 8min, collect hydrolyzed solution, successively through diatomite filtration, namely ultrafiltration obtains transparent, stable queen bee embryo hydrolyzed solution,
Above-mentioned traditional vacuum condition is temperature 6 DEG C, vacuum tightness-0.02MPa;
Above-mentioned biological demulsifying agent is glycolipid class biological demulsifying agent, and it is specially rhamnolipid;
3) the base wine of queen bee embryo hydrolyzed solution quality 1 times is got, add peracetic acid soln wherein, peroxyacetic acid concentration is made to be 400ppm, Homogeneous phase mixing, carries out ultrasonication 5min under power 200W, frequency 30KHz condition, treated base wine and queen bee embryo hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 25 DEG C, then by the process of continuous high voltage pulse treatment chamber, leaves standstill, filters and obtain queen bee embryo health promoting wine;
Above-mentioned base wine is 40 degree of white wine;
The mass percent concentration of above-mentioned peracetic acid soln is 10%;
Above-mentioned pulsed electric field treatment condition are strength of electric field 25kV/cm, umber of pulse 40.
Embodiment three:
A kind of queen bee embryo health promoting wine, its preparation method comprises the following steps:
1) by fresh queen bee embryo and solion in mass ratio 1:1.5 mix, in-22 DEG C of freezing 30min, broken, percentage of damage is 100%, broken thing particle diameter 300 μm, regulate broken thing pH value to be 7 with lactic acid, be warming up to 50 DEG C, add the prozyme of fresh queen bee embryo quality 0.18% wherein, mix, in the degassed de-raw meat 15min of vacuum tightness-0.09MPa, leave standstill, continue enzymolysis 15min and obtain enzymolysis solution;
Above-mentioned solion is the aqueous solution containing sodium ion 30mg/L, zine ion 22mg/L, potassium ion 20mg/L, calcium ion 14mg/L and magnesium ion 11mg/L;
Above-mentioned prozyme quality group becomes: flavor protease: aspartic protease: lipase=4:3:0.5;
2) enzymolysis solution is put 7000r/min traditional vacuum 10min in vacuum centrifuge, be separated from top to bottom and obtain free-fat, milk sap, hydrolyzed solution and residue, it is 55 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent breakdown of emulsion 40min of milk sap quality 0.12%, in 6000r/min traditional vacuum 2 times after breakdown of emulsion, interval time 3h, each centrifugal 15min, merge three centrifugal hydrolyzed solutions obtained, get the egg white of its quality 0.4%, put into stirrer and stir 12min in rotating speed 1000r/min, until egg white all becomes foam obtain egg white foams, egg white foams is added in the hydrolyzed solution merging and obtain, normal pressure boils 8min, be down to room temperature, in 7000r/min traditional vacuum 10min, collect hydrolyzed solution, successively through diatomite filtration, namely ultrafiltration obtains transparent, stable queen bee embryo hydrolyzed solution,
Above-mentioned traditional vacuum condition is temperature 10 DEG C, vacuum tightness-0.04MPa;
The quality group of above-mentioned biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=6:5:3;
3) the base wine of queen bee embryo hydrolyzed solution quality 3 times is got, add peracetic acid soln wherein, peroxyacetic acid concentration is made to be 480ppm, Homogeneous phase mixing, carries out ultrasonication 10min under power 400W, frequency 40KHz condition, treated base wine and queen bee embryo hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 35 DEG C, then by the process of continuous high voltage pulse treatment chamber, leaves standstill, filters and obtain queen bee embryo health promoting wine;
Above-mentioned base wine is 60 degree of white wine;
The mass percent concentration of above-mentioned peracetic acid soln is 30%;
Above-mentioned pulsed electric field treatment condition are strength of electric field 30kV/cm, umber of pulse 50.
Embodiment four:
A kind of drone pupae health promoting wine, its preparation method comprises the following steps:
1) by fresh drone pupae and solion in mass ratio 1:0.8 mix, in-19 DEG C of freezing 23min, broken, percentage of damage is 98%, broken thing particle diameter 230 μm, regulate broken thing pH value to be 5.5 with lactic acid, be warming up to 43 DEG C, add the prozyme of fresh drone pupae quality 0.12% wherein, mix, in the degassed de-raw meat 12min of vacuum tightness-0.07MPa, leave standstill, continue enzymolysis 12min and obtain enzymolysis solution;
Above-mentioned solion is the aqueous solution containing sodium ion 27mg/L, zine ion 19mg/L, potassium ion 17mg/L, calcium ion 12mg/L and magnesium ion 9mg/L;
Above-mentioned prozyme quality group becomes: flavor protease: aspartic protease: lipase=2:1:0.3;
2) enzymolysis solution is put 5500r/min traditional vacuum 8min in vacuum centrifuge, be separated from top to bottom and obtain free-fat, milk sap, hydrolyzed solution and residue, it is 48 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent breakdown of emulsion 25min of milk sap quality 0.09%, in 4500r/min traditional vacuum 2 times after breakdown of emulsion, interval time 1.5h, each centrifugal 12min, merge three centrifugal hydrolyzed solutions obtained, get the egg white of its quality 0.2%, put into stirrer and stir 10min in rotating speed 850r/min, until egg white all becomes foam obtain egg white foams, egg white foams is added in the hydrolyzed solution merging and obtain, normal pressure boils 6min, be down to room temperature, in 5500r/min traditional vacuum 8min, collect hydrolyzed solution, successively through diatomite filtration, namely ultrafiltration obtains transparent, stable drone pupae hydrolyzed solution,
Upper traditional vacuum condition is temperature 8 DEG C, vacuum tightness-0.02MPa;
Above-mentioned biological demulsifying agent is lipopeptid class biological demulsifying agent;
3) the base wine of drone pupae hydrolyzed solution quality 1 times is got, add peracetic acid soln wherein, peroxyacetic acid concentration is made to be 420ppm, Homogeneous phase mixing, carries out ultrasonication 7min under power 250W, frequency 30KHz condition, treated base wine and drone pupae hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 28 DEG C, then by the process of continuous high voltage pulse treatment chamber, leaves standstill, filters and obtain drone pupae health promoting wine;
Above-mentioned base wine is 45 degree of white wine;
The mass percent concentration of above-mentioned peracetic acid soln is 15%;
Above-mentioned pulsed electric field treatment condition are strength of electric field 27kV/cm, umber of pulse 43.
Embodiment five:
A kind of drone pupae health promoting wine, its preparation method comprises the following steps:
1) by fresh drone pupae and solion in mass ratio 1:1.3 mix, in-21 DEG C of freezing 28min, broken, percentage of damage is 99%, broken thing particle diameter 280 μm, regulate broken thing pH value to be 6.5 with lactic acid, be warming up to 48 DEG C, add the prozyme of fresh drone pupae quality 0.16% wherein, mix, in the degassed de-raw meat 14min of vacuum tightness-0.09MPa, leave standstill, continue enzymolysis 14min and obtain enzymolysis solution;
Above-mentioned solion is the aqueous solution containing sodium ion 29mg/L, zine ion 21mg/L, potassium ion 19mg/L, calcium ion 14mg/L and magnesium ion 11mg/L;
Above-mentioned prozyme quality group becomes: flavor protease: aspartic protease: lipase=3.5:2.5:0.4;
2) enzymolysis solution is put 6500r/min traditional vacuum 10min in vacuum centrifuge, be separated from top to bottom and obtain free-fat, milk sap, hydrolyzed solution and residue, it is 53 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent breakdown of emulsion 35min of milk sap quality 0.11%, in 5500r/min traditional vacuum 2 times after breakdown of emulsion, interval time 2.5h, each centrifugal 14min, merge three centrifugal hydrolyzed solutions obtained, get the egg white of its quality 0.4%, put into stirrer and stir 12min in rotating speed 950r/min, until egg white all becomes foam obtain egg white foams, egg white foams is added in the hydrolyzed solution merging and obtain, normal pressure boils 7min, be down to room temperature, in 6500r/min traditional vacuum 10min, collect hydrolyzed solution, successively through diatomite filtration, namely ultrafiltration obtains transparent, stable drone pupae hydrolyzed solution,
Above-mentioned traditional vacuum condition is temperature 9 DEG C, vacuum tightness-0.04MPa;
Above-mentioned biological demulsifying agent is that cell wall is in conjunction with class biological demulsifying agent;
3) the base wine of drone pupae hydrolyzed solution quality 3 times is got, add peracetic acid soln wherein, peroxyacetic acid concentration is made to be 460ppm, Homogeneous phase mixing, carries out ultrasonication 9min under power 350W, frequency 40KHz condition, treated base wine and drone pupae hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 33 DEG C, then by the process of continuous high voltage pulse treatment chamber, leaves standstill, filters and obtain drone pupae health promoting wine;
Above-mentioned base wine is 55 degree of white wine;
The mass percent concentration of above-mentioned peracetic acid soln is 25%;
Above-mentioned pulsed electric field treatment condition are strength of electric field 29kV/cm, umber of pulse 48.
The sensory evaluation test of embodiment six queen bee embryo health promoting wine of the present invention
24 personnel are invited to judge with the queen bee embryo health promoting wine of commercially available two kinds of similar identical date manufactured queen bee embryo health promoting wine prepared by the embodiment of the present invention 1, sense organ is given a mark, wherein specialty and each 12 of non-specialised staff, professional is young, middle aged, each 4 of old age, men and women half and half, non-specialised staff is juvenile, young, middle aged, each 3 of old age, and men and women half and half; Marking comprises outward appearance (20 points), quality (25 points), local flavor (30 points), mouthfeel (25 points) four aspects, and marking personnel independently carry out, and are independent of each other, accurate to ensure judging result.Add up judging result, equal score value gets approximation, retains integer, specifically in table 1:
Table 1: sensory evaluation statistics
Note: show significant difference (P < 0.05) with marking different lowercase alphabet in a line, mark different capitalization and represent that difference extremely significantly (P < 0.01), indicate same letter and represent difference not significantly (P > 0.05).
Above result shows, any one all obviously will be better than commercially available queen bee embryo health promoting wine to queen bee embryo health promoting wine prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, particularly outward appearance clarity, local flavor and mouthfeel are fabulous, and also the human consumer of applicable Different age group, different hierarchy of consumption eats simultaneously.
It should be noted that: queen bee embryo health promoting wine prepared by embodiment of the present invention 2-3 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.
Embodiment seven queen bee embryo health promoting wine of the present invention is on the impact of immunity of organisms
1 experiment purpose
By exercise tolerance test (mouse forced swimming), verify raising immunizing power, the antifatigue effect of queen bee embryo health promoting wine of the present invention.
2 experiment materials and reagent
2.1 for reagent thing:
Commercially available queen bee embryo health promoting wine (G1); Commercially available queen bee embryo health promoting wine (G2); Queen bee embryo health promoting wine (G3-G5) prepared by embodiment of the present invention 1-3.
2.2 reagent:
Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing; The vitriol oil (AR), Nanjing Chemistry Reagent Co., Ltd.; Physiological saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..
3. laboratory animal
ICR mouse, ♂, cleaning grade, body weight 18-22g, is provided by Ningxia Medical University's comparative medicine center, the free diet of experimental session mouse.
4. key instrument
Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed whizzer: 5804R type, Eppendrof company; Water-bath: DK-S26 type, the grand experimental installation company limited of upper Nereid; Electronic scale: BS224S type, Sartorius company; Stopwatch, thermometer
5. experiment grouping
5.1 dosage groupings and given the test agent give the time and at random mouse are divided into 6 groups, often organize 10,1st group to the 5th group respectively to the medicine of G1 ~ G5,6th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.
5.2 sample preparations the 1st group to the 7th group: take 2.25g drug sample, be assigned to 150ml with distilled water; Blank group: distilled water 150ml.
6. experimental technique
After 6.1 swimming with a load attached to the body experiment last administration 30min, put mouse in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of mouse root of the tail portion load 5% body weight, and the swimming of record mouse starts to the dead time, as mouse swimming time.
After 6.2 mice serum urea measure last administration 30min, be not swimming with a load attached to the body 90min in the water of 30 DEG C in temperature, eyeball blood sampling 0.5mL (not adding antithrombotics) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, the centrifugal 15min of 2000r/min after hemopexis, gets serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.
After the mensuration last administration 30min of 6.3 liver starch, be not swimming with a load attached to the body 90min in the water of 25 ± 1 DEG C in temperature, cervical dislocation puts to death mouse, clean with physiological saline, and with after filter paper suck dry moisture, accurately taking liver 100mg, liver starch detection kit detects Mouse Liver glycogen content.
Take a blood sample after the mensuration last administration 30min of 6.4 blood lactase acid, then do not bear a heavy burden stops after temperature is the water went swimming 10min of 30 DEG C.Lactic acid instrument measuring method: after rest 20min, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid before swimming, after swimming, after swimming respectively, the smudge cells lactic acid instrument that fully vibrates immediately measures.(the blood lactase acid value of the rear 20min of blood lactase acid value+2 × swimming of the rear 0min of the front blood lactase acid value of blood lactase acid area under curve=5 × swimming+3 × swimming)
7. observation index walking weight load, blood lactase acid, urea, glycogen initial value
8. statistical method experimental data x ± s represents, employing t inspection is compared between organizing
9. experimental result
9.1 queen bee embryo health promoting wine of the present invention are on the impact of Mouse Weight
Each group of mouse is after giving G1 ~ G5 medicine, before, in, shown in post-weight sees the following form respectively, the original body mass of each group mouse and increase weight body weight and the more equal no difference of science of statistics of control group (P > 0.05), show G1 ~ G5 medicine all without obvious toxicity.Experimental result refers to table 2.
The original body mass of table 2 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight
9.2 queen bee embryo health promoting wine of the present invention are on the impact of mice burden swimming time
After per os gives mouse G1 ~ G5 medicine, G1 ~ G2 medicine compares with blank group, can obviously extend the mice burden swimming time, there is significant difference (P < 0.05), queen bee embryo health promoting wine G3 ~ G5 medicine of the present invention compares with blank group, can the significant prolongation mice burden swimming time, there is pole significant difference (P < 0.01), and be obviously better than G1 ~ G2 medicine.The results detailed in Table 3.
Table 3 queen bee embryo health promoting wine is on the impact of mice burden swimming time
" * " p<0.05vs blank;
" * * " p<0.01vs blank;
9.3 queen bee embryo health promoting wine of the present invention are on the impact of blood lactase acid before and after mouse movement
After per os gives mouse queen bee embryo health promoting wine of the present invention, queen bee embryo health promoting wine G3 ~ G5 medicine of the present invention to compare with control group blood lactase acid area under curve after mouse movement significant difference (P < 0.05), decrease though G1 ~ G2 medicine group Mouse Blood lactic acid area under curve compares with control group, and no difference of science of statistics (P > 0.05).The results are shown in Table 4.
Table 4 queen bee embryo health promoting wine of the present invention is on the impact of blood lactase acid level before and after mouse movement
" * " p<0.05vs blank;
9.4 queen bee embryo health promoting wine of the present invention are on the impact of Mouse Liver glycogen
After per os gives mouse G1 ~ G5 medicine, G1 ~ G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), queen bee embryo health promoting wine G3 ~ G5 medicine of the present invention compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is obviously better than G1 ~ G2 medicine.The results detailed in Table 5.
Table 5 queen bee embryo health promoting wine of the present invention is on the impact of Mouse Liver glycogen content
" * " p<0.05vs blank;
" * * " p<0.01vs blank;
9.5 queen bee embryo health promoting wine of the present invention are on the impact of mice serum urea
After per os gives mouse G1 ~ G5 medicine, G1 ~ G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), queen bee embryo health promoting wine G3 ~ G5 medicine of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction, has pole significant difference (P < 0.01), and is obviously better than G1 ~ G2 medicine.The results detailed in Table 6.
Table 6 queen bee embryo health promoting wine of the present invention is on the impact of mice serum urea content
" * " p<0.05vs blank;
" * * " p<0.01vs blank;
10. experiment conclusion
This experiment is mainly through mice burden swimming experiment, and the deposit simultaneously detecting Mouse Liver glycogen observes the effect that queen bee embryo health promoting wine of the present invention improves immunizing power, antifatigue.Preliminary Results shows below:
1, G3 ~ G5 queen bee embryo health promoting wine of the present invention all can extend the mice burden swimming time (P < 0.01), and successful is better than the queen bee embryo health promoting wine of other G1 ~ G2.
2, biochemistry detection aspect display, the lactic acid content that after each dosage group of G3 ~ G5 queen bee embryo health promoting wine of the present invention all can reduce motion, in mice serum, glucose anaerobic glycolysis produces, compare with control group and have significant difference (P < 0.05), and although the queen bee embryo health promoting wine of other G1 ~ G2 also can reduce the lactic acid content that in the rear mice serum of motion, glucose anaerobic glycolysis produces, but compare with control group, no difference of science of statistics (P > 0.05);
3, each dosage group of G3 ~ G5 queen bee embryo health promoting wine of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and successful is better than the queen bee embryo health promoting wine of other G1 ~ G2;
4, high lithemia model finds, G3 ~ G5 queen bee embryo health promoting wine of the present invention significantly can reduce the content (P < 0.01) of the rear urea in serum of mouse swimming, and successful is better than other G1 ~ G2 queen bee embryo health promoting wine;
11. conclusions
Above-mentioned experiment proves that queen bee embryo health promoting wine of the present invention can significantly improve immunity of organisms, improve muscle power and the endurance of mouse, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to the fatigue that alleviation exercise load causes; The time that mice burden swimming to power exhausts can be extended.
Claims (10)
1. a preparation method for bee pupa worm health promoting wine, is characterized in that, comprises the following steps:
1) by fresh queen bee embryo or drone pupae and solion in mass ratio 1:0.5-1.5 mix, in-18--22 DEG C of freezing 20-30min, broken, percentage of damage is 98-100%, broken thing particle diameter 200-300 μm, regulate broken thing pH value to be 5-7 with lactic acid, be warming up to 40-50 DEG C, add the prozyme of fresh queen bee embryo or drone pupae quality 0.10-0.18% wherein, mix, in the degassed de-raw meat 10-15min of vacuum tightness-0.07--0.09MPa, leave standstill, continue enzymolysis 10-15min and obtain enzymolysis solution;
Described prozyme quality group becomes: flavor protease: aspartic protease: lipase=2-4:1-3:0.3-0.5;
2) enzymolysis solution is put 5000-7000r/min traditional vacuum 8-10min in vacuum centrifuge, be separated from top to bottom and obtain free-fat, milk sap, hydrolyzed solution and residue, be 45-55 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent breakdown of emulsion 20-40min of milk sap quality 0.08-0.12%, in 4000-6000r/min traditional vacuum 2 times after breakdown of emulsion, interval time 1-3h, each centrifugal 10-15min, merge three centrifugal hydrolyzed solutions obtained, get the egg white of its quality 0.2-0.4%, put into stirrer and stir 10-12min in rotating speed 800-1000r/min, until egg white all becomes foam obtain egg white foams, egg white foams is added in the hydrolyzed solution merging and obtain, normal pressure boils 5-8min, be down to room temperature, in 5000-7000r/min traditional vacuum 8-10min, collect hydrolyzed solution, successively through diatomite filtration, namely ultrafiltration obtains transparent, stable queen bee embryo or drone pupae hydrolyzed solution,
3) queen bee embryo or drone pupae hydrolyzed solution quality 1-3 base wine is doubly got, add peracetic acid soln wherein, peroxyacetic acid concentration is made to be 400-480ppm, Homogeneous phase mixing, carries out ultrasonication 5-10min under power 200-400W, frequency 30-40KHz condition, treated base wine and queen bee embryo or drone pupae hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 25-35 DEG C, then by the process of continuous high voltage pulse treatment chamber, leaves standstill, filters and obtain queen bee embryo or drone pupae health promoting wine.
2. the preparation method of bee pupa worm health promoting wine as claimed in claim 1, it is characterized in that, step 1) described solion is the aqueous solution containing sodium ion 25-30mg/L, zine ion 17-22mg/L, potassium ion 15-20mg/L, calcium ion 12-14mg/L and magnesium ion 9-11mg/L.
3. the preparation method of bee pupa worm health promoting wine as claimed in claim 1, is characterized in that, step 2) described traditional vacuum condition is temperature 6-10 DEG C, vacuum tightness-0.02--0.04MPa.
4. the preparation method of bee pupa worm health promoting wine as claimed in claim 1, is characterized in that, step 2) described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall are in conjunction with the one or more combination in class biological demulsifying agent.
5. the preparation method of bee pupa worm health promoting wine as claimed in claim 4, it is characterized in that, the quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=5-7:4-6:2-4.
6. the preparation method of bee pupa worm health promoting wine as claimed in claim 5, is characterized in that, described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl glycoside; The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl glycoside=3-5:1-2.
7. the preparation method of bee pupa worm health promoting wine as claimed in claim 1, is characterized in that, step 3) described base wine is 40-60 degree white wine.
8. the preparation method of bee pupa worm health promoting wine as claimed in claim 1, is characterized in that, step 3) mass percent concentration of described peracetic acid soln is 10-30%.
9. the preparation method of bee pupa worm health promoting wine as claimed in claim 1, is characterized in that, step 3) described pulsed electric field treatment condition are strength of electric field 25-30kV/cm, umber of pulse 40-50.
10. the bee pupa worm health promoting wine that obtains of preparation method as arbitrary in claim 1-9.
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| CN105919071A (en) * | 2016-04-21 | 2016-09-07 | 北京东方兴企食品工业技术有限公司 | Functional compound protein composition and preparation method thereof |
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