CN104886335A - Composite enzymolysis technology for drone pupae - Google Patents
Composite enzymolysis technology for drone pupae Download PDFInfo
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- CN104886335A CN104886335A CN201410073585.XA CN201410073585A CN104886335A CN 104886335 A CN104886335 A CN 104886335A CN 201410073585 A CN201410073585 A CN 201410073585A CN 104886335 A CN104886335 A CN 104886335A
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Abstract
The invention puts forward a composite enzymolysis technology for drone pupae. The bee pupa body wall contains chitin, and the body mainly contains protein and fat internally. The invention adopts a supercritical technology to remove fat from drone pupae, a tyrosinase inhibitor is employed to prevent enzymolysis browning, by means of enzyme catalysis specificity, the drone pupae are enzymatically hydrolyzed into small molecule peptides, and separation is carried out according to whether the small molecule peptides are water soluble so as to obtain two different small molecule peptides for further research and development. The innovation point of the invention lies in that the composite enzyme has high temperature resistance and similar starting temperature, can degrade bee pupa bodies overall within the same temperature and pH range, and prevent enzymolysis liquid discoloration to form non-water soluble and water soluble small molecule peptide and amino acid, and then freeze drying is carried out obtain fine powder for sealing and low temperature storage.
Description
Technical field
The invention belongs to the fermented by biological enzyme manufacture field of food high protein raw material, particularly several enzyme compound action enzymolysis drone pupae technology.
Background technology
China's Honeybee enriches, cultured area is wide, bee pupa annual production is large, drying drone pupae proportion also rises year by year, but less to the research of drone pupae at present, the exploitation of drone pupae relatively lag behind, drone pupae processing still rests on the primary stage, often be processed into larva wine, or make dry powder and dress up capsule and introduce to the market as health food, or using fresh pupa or dry powder directly as material outlet, technology content and the added value of product are low, most drone pupae resource is effectively developed not yet, and the industrialization having a strong impact on honeybee develops in a healthy way.The present invention is directed to the compositional character of drone pupae, develop a kind of combination zymolysis technique of applicable enzymolysis drone pupae albumen, effective raising enzymolysis finished product yield, optimize process conditions, greatly improve drone pupae added value, extend bee product industrial chain, for technical foundation is established in bee product industrialized development.
Summary of the invention
The present invention proposes a kind of technology of enzymolysis drone pupae, drone pupae body wall is containing chitin, mainly albumen and fat in body, the present invention adopts supercritical technology to be deviate from by the fat in drone pupae, utilizes TYR enzyme inhibitor to prevent the brown stain of enzymolysis, utilizes enzymatic selectivity, drone pupae enzymolysis is become small-molecular peptides, be separated according to whether water-soluble small-molecular peptides is, obtain the small-molecular peptides that two classes are different, for follow-up research and development.Innovative point complex enzyme of the present invention has high temperature resistant, similar start-up temperature, can to the degraded of bee pupa body entirety within the scope of same temperature and pH, and prevention enzymolysis liquid look becomes, and forms soluble small molecular peptide and amino acid.
Technical scheme of the present invention: adopt supercritical technology to slough fat, super critical condition temperature 45 ~ 55 DEG C, extracting pressure 30 ~ 40MPa, separating pressure 4 ~ 8MPa, extraction time 60 ~ 120min.Complex enzyme is by weight preparation: neutral proteinase 30 ~ 50 parts, 20 ~ 40 parts, trypsase, papain 10 ~ 15 parts, chitinase 3 ~ 5 parts, TYR enzyme inhibitor 5 ~ 10 parts, the drying drone pupae of material-water ratio 1:10 ~ 16(degreasing: deionized water=1:10 ~ 16), reaction temperature 35 ~ 50 DEG C, reaction time 3 ~ 5h, pH value 7.0 ~ 8.0, complex enzyme addition 0.4 ~ 0.8%, use active carbon coarse filtration after enzymolysis, then use the filter of 0.4 ~ 0.8 μm of film essence, obtain clear transparent solutions, solution adopts the drying one-tenth powder of Freeze Drying Technique, and packaging of aluminium foil bag is moistureproof.
Detailed description of the invention
For definitely stating the present invention, now giving an actual example and being illustrated:
The overcritical filler of example 1 keeps upper and lower gas porosity consistent, temperature 45 C, extracting pressure 35MPa, separating pressure 4MPa, extraction time 100min.Complex enzyme is by weight preparation: neutral proteinase 45 parts, 30 parts, trypsase, papain 15 parts, chitinase 5 parts, TYR enzyme inhibitor 5 parts, the drying drone pupae of material-water ratio 1:12(degreasing: deionized water=1:12), reaction temperature 45 DEG C, reaction time 4h, pH value 7.5, complex enzyme addition 0.6%, use active carbon coarse filtration after enzymolysis, then with the filter of 0.5 μm of film essence, obtain clear transparent solutions, solution adopts the drying one-tenth powder of Freeze Drying Technique, and packaging of aluminium foil bag is moistureproof.
The overcritical filler of example 2 keeps upper and lower gas porosity consistent, temperature 50 C, extracting pressure 36MPa, separating pressure 4MPa, extraction time 120min.Complex enzyme is by weight preparation: neutral proteinase 45 parts, 30 parts, trypsase, papain 15 parts, chitinase 5 parts, TYR enzyme inhibitor 5 parts, the drying drone pupae of material-water ratio 1:14(degreasing: deionized water=1:14), reaction temperature 50 DEG C, reaction time 3h, pH value 7.5, complex enzyme addition 0.8%, use active carbon coarse filtration after enzymolysis, then with the filter of 0.6 μm of film essence, obtain clear transparent solutions, solution adopts the drying one-tenth powder of Freeze Drying Technique, and packaging of aluminium foil bag is moistureproof.
Claims (4)
1. the present invention proposes a kind of complex enzyme hydrolysis drone pupae technology, it is characterized in that, adopt supercritical technology to carry out degreasing, super critical condition before enzymolysis: temperature 45 ~ 55 DEG C, extracting pressure 30 ~ 40MPa, separating pressure 4 ~ 8MPa.
2. a kind of complex enzyme hydrolysis drone pupae technology according to right 1, is characterized in that, complex enzyme is by weight preparation: neutral proteinase 30 ~ 50 parts, 20 ~ 30 parts, trypsase, papain 5 ~ 15 parts, chitinase 3 ~ 12 parts, network protease inhibitors 3 ~ 15 parts.
3. a kind of complex enzyme hydrolysis drone pupae technology according to claim 1 and 2, is characterized in that, the drying drone pupae of material-water ratio 1:10 ~ 16(degreasing: deionized water=1:10 ~ 16), reaction temperature 35 ~ 50 DEG C, reaction time 3 ~ 5h, pH value 7.0 ~ 8.0, complex enzyme addition 0.4 ~ 0.8%.
4. according to a kind of complex enzyme hydrolysis drone pupae technology of the claims, it is characterized in that, use active carbon coarse filtration after enzymolysis, then use the filter of 0.2 ~ 0.8 μm of film essence, obtain clear transparent solutions, solution adopts the drying one-tenth powder of Freeze Drying Technique.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410073585.XA CN104886335A (en) | 2014-03-03 | 2014-03-03 | Composite enzymolysis technology for drone pupae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410073585.XA CN104886335A (en) | 2014-03-03 | 2014-03-03 | Composite enzymolysis technology for drone pupae |
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| Publication Number | Publication Date |
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| CN104886335A true CN104886335A (en) | 2015-09-09 |
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| CN201410073585.XA Pending CN104886335A (en) | 2014-03-03 | 2014-03-03 | Composite enzymolysis technology for drone pupae |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441286A (en) * | 2016-02-03 | 2016-03-30 | 北京蜜蜂堂生物医药股份有限公司 | Bee pupa worm health care wine and preparation method thereof |
| CN105533595A (en) * | 2015-12-09 | 2016-05-04 | 杨佳林 | Bee pollen gel food and processing method thereof |
| CN108034683A (en) * | 2017-12-19 | 2018-05-15 | 华中农业大学 | A kind of preparation method of drone pupae small molecule anti-oxidation peptide and its application of drone pupae small molecule anti-oxidation peptide |
| CN108841905A (en) * | 2018-06-21 | 2018-11-20 | 国肽生物工程（常德）有限公司 | A kind of bee pupa protein peptides and preparation method thereof inhibited with ACE with anti-fatigue effect |
| CN109609577A (en) * | 2018-12-29 | 2019-04-12 | 武汉天天好生物制品有限公司 | A kind of bee tire peptide and its preparation process and application with anti-inflammatory effect |
| CN110720626A (en) * | 2019-10-31 | 2020-01-24 | 伊犁哈萨克自治州农业农村局 | Bee pupa puffed food and preparation method thereof |
-
2014
- 2014-03-03 CN CN201410073585.XA patent/CN104886335A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533595A (en) * | 2015-12-09 | 2016-05-04 | 杨佳林 | Bee pollen gel food and processing method thereof |
| CN105533595B (en) * | 2015-12-09 | 2019-08-02 | 杨佳林 | A kind of Bee Pollen gel food and its processing method |
| CN105441286A (en) * | 2016-02-03 | 2016-03-30 | 北京蜜蜂堂生物医药股份有限公司 | Bee pupa worm health care wine and preparation method thereof |
| CN108034683A (en) * | 2017-12-19 | 2018-05-15 | 华中农业大学 | A kind of preparation method of drone pupae small molecule anti-oxidation peptide and its application of drone pupae small molecule anti-oxidation peptide |
| CN108841905A (en) * | 2018-06-21 | 2018-11-20 | 国肽生物工程（常德）有限公司 | A kind of bee pupa protein peptides and preparation method thereof inhibited with ACE with anti-fatigue effect |
| CN109609577A (en) * | 2018-12-29 | 2019-04-12 | 武汉天天好生物制品有限公司 | A kind of bee tire peptide and its preparation process and application with anti-inflammatory effect |
| CN110720626A (en) * | 2019-10-31 | 2020-01-24 | 伊犁哈萨克自治州农业农村局 | Bee pupa puffed food and preparation method thereof |
| CN110720626B (en) * | 2019-10-31 | 2022-11-04 | 伊犁哈萨克自治州农业农村局 | Bee pupa puffed food and preparation method thereof |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150909 |
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| WD01 | Invention patent application deemed withdrawn after publication |