CN108034683A - A kind of preparation method of drone pupae small molecule anti-oxidation peptide and its application of drone pupae small molecule anti-oxidation peptide - Google Patents
A kind of preparation method of drone pupae small molecule anti-oxidation peptide and its application of drone pupae small molecule anti-oxidation peptide Download PDFInfo
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Abstract
The present invention provides a kind of preparation method of drone pupae small molecule anti-oxidation peptide, belong to functional food technical field, drone pupae powder is mixed with water, is homogenized, after the pH value for adjusting homogenate, digested after being mixed with complex enzyme, complex enzyme includes endo protease and exoproteinase；Enzymolysis liquid is centrifuged, is filtered, obtains filtrate, filtrate is subjected to first step ultra-filtration and separation peptide fragment；Second step ultrafiltration desalination is carried out again；It is freeze-dried after being concentrated under reduced pressure, obtains drone pupae small molecule anti-oxidation peptide.Method using the present invention can reduce the formation of bitter peptides in enzymolysis liquid, and can increase the content of the small-molecular peptides in enzymolysis liquid, and the molecular weight of obtained drone pupae small molecule anti-oxidation peptide is less than 1ku, and the antioxygenic property with high activity.
Description
Technical field
The present invention relates to functional food technical field, more particularly to a kind of preparation side of drone pupae small molecule anti-oxidation peptide
The application of method and its drone pupae small molecule anti-oxidation peptide.
Background technology
Drone pupae refers to unfertilized egg of the queen bee in drone house property, and the pupal cell hatch through worker bee, develop, sprouted wings, its is general
Individual when referring to male honeybee development to 22~23 age in days, pupa at this time is ripe, but not yet sprouts wings, and contains what is extremely enriched
Nutriment, is the discarded object during royal jelly and its product.China bee colony can produce the dry drone pupae of more than 4000t every year.But
For a long time, the bee pupa product of domestic production is mainly bee pupa albumen powder, is only simply processed, or will be directly as raw material
Outlet, the technology content and added value of product are low, and the resource of most bee pupas is not developed effectively yet.
Correlative study shows that hydrolysis end-product of the protein in digestion under albumen enzyme effect is mostly 2~4
The small peptide of a amino acid residue composition, molecular weight is below 1ku.Because small peptide is simple in structure, molecular weight is small, unsaturated, so energy
Enough it is directly entered by the infiltration of cell membrane with original shape into the cell, without re-digesting, it is not required that expend energy.Therefore
Absorption, conversion and the utilization of small-molecular peptides, are all efficient, complete, thoroughly.
104886335 A of Chinese invention patent CN disclose a kind of scheme of complex enzyme hydrolysis drone pupae, and the invention is using super
Critical technology removes fat in drone pupae, recycles complex enzyme (neutral proteinase, trypsase, papain, chitin
Enzyme, casease inhibitor) enzymolysis, use activated carbon coarse filtration after enzymolysis, then with 0.2~0.8 μm of film refined filtration, according to small-molecular peptides water
Separated whether molten, obtain the different small-molecular peptides of two classes.But the peptide that this method is prepared into is still the very big peptide of molecular weight
(molecular weight is tens of thousands of or even hundreds of thousands), is not small-molecular peptides.
The content of the invention
It is using the present invention object of the present invention is to provide a kind of preparation method of drone pupae small molecule anti-oxidation peptide
Method can obtain the small-molecular peptides that molecular weight is less than 1ku.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The present invention provides a kind of preparation method of drone pupae small molecule anti-oxidation peptide, comprise the following steps：
1) drone pupae powder is mixed with water, be homogenized, after the pH value for adjusting homogenate is 6~7, mixed with complex enzyme, 50
1~3h is digested at~60 DEG C, obtains enzymolysis liquid, the complex enzyme includes endo protease and exoproteinase；
2) enzymolysis liquid that the step 1) obtains is centrifuged, obtained supernatant is filtered, is filtered
Liquid, the speed of the centrifugation is 4000~5000rpm, and the filtering is carried out using filter cloth, the aperture of the filter cloth for 1~
5μm；
3) filtrate for obtaining the step 2) carries out first step ultra-filtration and separation peptide fragment, obtains peptide fragment molecular weight ＜ 1ku's
Ultrafiltrate, the molecular cut off for the ultrafiltration membrane that the first step ultrafiltration uses successively is 10ku, 5ku, 3ku and 1ku；
4) ultrafiltrate for the peptide fragment molecular weight ＜ 1ku for obtaining the step 3) carries out second step ultrafiltration desalination, is cut
Liquid is stayed, the molecular cut off for the ultrafiltration membrane that the second step ultrafiltration uses is 0.15~0.3ku；
5) it is freeze-dried after the trapped fluid that the step 4) obtains is concentrated under reduced pressure, it is anti-oxidant obtains drone pupae small molecule
Peptide.
Preferably, the total enzyme activity of the step 1) complex enzyme and the mass ratio of drone pupae powder are (1000~2000) U:1g,
The enzyme activity ratio of the endo protease and exoproteinase is (1~3):(1~3).
Preferably, the step 1) endo protease includes alkali protease, papain, trypsase and neutral egg
One or more in white enzyme.
Preferably, the exoproteinase includes the one or more in flavor protease, carboxypeptidase and aminopeptidase.
Preferably, the granularity of step 1) the drone pupae powder is 10~100 μm.
Preferably, the quality of step 1) the drone pupae powder and the volume ratio of water are 1g:(10~15) mL.
Preferably, the time that the step 2) centrifuges is 10~15min.
Preferably, the condition of the step 3) first step ultrafiltration includes：Filtrate flow velocity is in the first step ultra-filtration process
0.5~10L/H, the membrane component pressure of the first step ultrafiltration is 0~0.8MPa, before first step ultrafiltration terminates add pure water into
Row washing, until the permeate of outflow is colourless；
The condition of the second step ultrafiltration includes：The flow velocity of the second step ultrafiltration is 0.5~10L/H, second step ultrafiltration
Membrane component pressure be 0~1.2MPa, before second step ultrafiltration terminates add pure water washed, until outflow permeate be
It is colourless.
Present invention also offers the drone pupae small molecule anti-oxidation peptide that above-mentioned preparation method is prepared, the drone pupae are small
The molecular weight of molecule anti-oxidation peptide is 0.15~1ku.
The drone pupae small molecule anti-oxidation peptide that is prepared present invention also offers the preparation method described in such scheme or
Application of the drone pupae small molecule anti-oxidation peptide in anti-oxidation medicine and/or food is prepared described in such scheme.
The present invention provides a kind of preparation method of drone pupae small molecule anti-oxidation peptide, comprise the following steps：
1) drone pupae powder is mixed with water, be homogenized, after the pH value for adjusting homogenate, mixed with complex enzyme, at 50~60 DEG C
1~3h of lower enzymolysis, obtains enzymolysis liquid, and the complex enzyme includes endo protease and exoproteinase；2) step 1) is obtained
To enzymolysis liquid be centrifuged, obtained supernatant is filtered, obtains filtrate, the speed of the centrifugation is
4000~5000rpm, the filtering are carried out using filter cloth, and the aperture of the filter cloth is 1~5 μm；3) step 2) is obtained
Filtrate carry out first step ultra-filtration and separation peptide fragment, obtain the ultrafiltrate of peptide fragment molecular weight ＜ 1ku, the first step ultrafiltration makes successively
The molecular cut off of ultrafiltration membrane is 10ku, 5ku, 3ku and 1ku；4) the peptide fragment molecular weight ＜ 1ku for obtaining the step 3)
Ultrafiltrate carry out second step ultrafiltration desalination, obtain trapped fluid, the molecular cut off for the ultrafiltration membrane that the second step ultrafiltration uses
For 0.15~0.3ku；5) after the trapped fluid that the step 4) obtains being concentrated under reduced pressure, is freeze-dried, drone pupae small molecule is obtained
Anti-oxidation peptide.
Drone pupae powder is obtained enzymolysis liquid by the present invention after complex enzyme hydrolysis, and enzymolysis liquid includes each molecular weight peptide, fat, salt
Class, residue etc.；By obtained enzymolysis liquid after first step ultrafiltration, the peptide fragment molecular weight ＜ 1ku in obtained ultrafiltrate, and
Also contain salt；Ultrafiltrate, except desalting, after being concentrated under reduced pressure and being freeze-dried, obtains molecular weight as 0.15 after second step ultrafiltration
The peptide of~1ku.
The results show of the embodiment of the present invention：The method of drone pupae small molecule anti-oxidation peptide is prepared using the present invention to be subtracted
The formation of bitter peptides in few enzymolysis liquid, and the content of the small-molecular peptides in enzymolysis liquid can be increased, small point of obtained drone pupae
The molecular weight of sub- anti-oxidation peptide is less than 1ku, and the antioxygenic property with high activity.
Brief description of the drawings
Fig. 1 is the content of different molecular weight peptide in enzymolysis liquid；
Fig. 2 is different molecular weight peptide oxygen radical absorbability (ORAC)；
Fig. 3 is the Scavenging activity of different molecular weight Peptide D PPH.
Embodiment
The present invention provides a kind of preparation method of drone pupae small molecule anti-oxidation peptide, comprise the following steps：
1) drone pupae powder is mixed with water, be homogenized, after the pH value for adjusting homogenate is 6~7, mixed with complex enzyme, 50
1~3h is digested at~60 DEG C, obtains enzymolysis liquid, the complex enzyme includes endo protease and exoproteinase；2) by the step
The rapid enzymolysis liquid 1) obtained is centrifuged, and obtained supernatant is filtered, and obtains filtrate, the speed of the centrifugation
It is 1~5 μm to spend for 4000~5000rpm, the aperture for filtering the filter cloth used；3) by the filtrate that the step 2) obtains into
Row first step ultra-filtration and separation peptide fragment, obtains the ultrafiltrate of peptide fragment molecular weight ＜ 1ku, the ultrafiltration that the first step ultrafiltration uses successively
The molecular cut off of film is 10ku, 5ku, 3ku and 1ku；4) ultrafiltrate for the peptide fragment molecular weight ＜ 1ku for obtaining the step 3)
Carry out second step ultrafiltration desalination, obtain trapped fluid, the molecular cut off of the ultrafiltration membrane that the second step ultrafiltration uses for 0.15~
0.3ku；5) it is freeze-dried after the trapped fluid that the step 4) obtains is concentrated under reduced pressure, obtains drone pupae small molecule anti-oxidation peptide.
The molecular weight of drone pupae small molecule anti-oxidation peptide provided by the invention is 0.15~1ku.
Drone pupae powder is mixed, is homogenized by the present invention with water, after the pH value for adjusting homogenate, is mixed with complex enzyme, 50~
1~3h is digested at 60 DEG C, obtains enzymolysis liquid, the complex enzyme includes endo protease and exoproteinase.
The present invention preferably freezes drone pupae, is milled after lyophilized, obtains drone pupae powder.In the present invention, the hero
Bee pupa is preferably unfertilized egg of the queen bee in drone house property, and the pupal cell hatch through worker bee, develop, sprouted wings, male honeybee, which develops, to arrive
Individual during 22~23 age in days, pupa at this time is ripe, but not yet sprouts wings.In the present invention, the granularity of the drone pupae powder is excellent
Elect 10~100 μm of mesh as, more preferably 15~25 μm, be most preferably 20 μm.The present invention is to the source of the drone pupae without spy
It is different to limit, the commercially available drone pupae routinely selected using those skilled in the art.
In the present invention, the quality of the drone pupae powder and the volume ratio of water are preferably 1g:(10~15) mL, more preferably
1g:(11~14) mL, is most preferably 1g:(10~15) mL.
The present invention is not particularly limited the condition of the homogenate, male using the conventional use of homogenate of those skilled in the art
The condition of bee pupa.
In the present invention, the pH value for adjusting homogenate is preferably 6~7, and more preferably 6.2~6.8, be most preferably
6.5.The present invention preferably adjusts the pH value of homogenate with dilute hydrochloric acid and sodium hydroxide solution.
In the present invention, the quality of the drone pupae powder and complex enzyme total enzyme activity ratio are preferably 1g:(1000~2000) U,
More preferably 1g:(1200~1800) U, is most preferably 1g:(1400~1600) U.In the present invention, the complex enzyme includes interior
Protease and exoproteinase are cut, the enzyme activity of the endo protease and exoproteinase is than being preferably (1~3):(1~3), more
Preferably (1.5~2.5):(1.5~2.5), are most preferably 2:2.
In the present invention, the endo protease preferably includes alkali protease, papain, trypsase and neutrality
One or more in protease, it is described it is several be specially two or more.When the endo protease is two kinds or two
During kind of the above, each group is graded enzyme activity mixing.
In the present invention, the exoproteinase preferably include one kind in flavor protease, carboxypeptidase and aminopeptidase or
It is several, it is described it is several be specially two or three, when the exoproteinase is two or three, each group enzyme activity of grading is mixed
Close.The present invention is not particularly limited the source of the endo protease and exoproteinase, normal using those skilled in the art
Advise the commercial product selected.
In the present invention, when the complex enzyme is digested, the peptide of long-chain both can be from peptide chain internal break, can also be from
Both ends are broken on the outside of peptide chain, the probability that increase small peptide is formed, and exoproteinase can cut off the hydrophobic ammonia on the outside of bitter peptides
Base acid, reduces the bitter taste of enzymolysis liquid and forms the flavor of uniqueness.
After the present invention mixes homogenate with complex enzyme, 1~3h is digested at 50~60 DEG C, obtains enzymolysis liquid.In this hair
In bright, the temperature of the enzymolysis is preferably 50~60 DEG C, more preferably 52~58 DEG C；The time of the enzymolysis is preferably 1.5~
2.5h, more preferably 2h.In the present invention, drone pupae powder obtains different molecular weight peptide, fats thing after complex enzyme zymohydrolysis
The mixtures such as matter, salt, insoluble sludge.
Obtained enzymolysis liquid is centrifuged the present invention, and obtained supernatant is filtered, obtains filtrate, described
The speed of centrifugation is 4000~5000rpm.
In the present invention, the speed of the centrifugation is 4000~5000rpm, is preferably 4200~4800rpm, more
Preferably 4300~4700rpm；The time of the centrifugation is preferably 10~15min, more preferably 11~14min, optimal
Elect 12~13min as.In the present invention, the centrifugation leaves the materials such as soluble peptides, salt, insoluble fats,
Cast out insoluble sludge.
In the present invention, the filtering is carried out using filter cloth, and the aperture of the filter cloth is preferably 1~5 μm, and more preferably 2
~4 μm, be most preferably 3 μm.In the present invention, insoluble fats are removed after the filtering, and particle diameter is more than 5 μm of impurity.
Obtained filtrate is carried out first step ultra-filtration and separation peptide fragment by the present invention, obtains the ultrafiltrate of peptide fragment molecular weight ＜ 1ku,
The molecular cut off for the ultrafiltration membrane that the first step ultrafiltration uses successively is 10ku, 5ku, 3ku and 1ku.
In the present invention, the condition of the first step ultrafiltration preferably includes：Filtrate flow velocity in the first step ultra-filtration process
For 0.5~10L/H, the membrane component pressure of the first step ultrafiltration is 0~0.8MPa, and pure water is added before first step ultrafiltration terminates
Washed, until the permeate of outflow is colourless so that the peptide that trapped fluid middle-molecular-weihydroxyethyl is less than molecular cut off is filtered as far as possible
Go out.
The ultrafiltrate of obtained peptide fragment molecular weight ＜ 1ku is carried out second step ultrafiltration desalination by the present invention, obtains trapped fluid, institute
The molecular cut off for stating the ultrafiltration membrane that second step ultrafiltration uses is 0.15~0.3ku.
In the present invention, the molecular cut off for the ultrafiltration membrane that the second step ultrafiltration uses is 0.15~0.3ku, is preferably
0.2~0.28ku, more preferably 0.22~0.25ku.
In the present invention, the condition of the second step ultrafiltration preferably includes：The flow velocity of the second step ultrafiltration for 0.5~
10L/H, the membrane component pressure of second step ultrafiltration is 0~1.2MPa, and pure water is added before second step ultrafiltration terminates and is washed, directly
Permeate to outflow is colourless so that salt constituents remove as far as possible in trapped fluid, leave 0.15~1ku of molecular cut off
Peptide.
The present invention is freeze-dried after obtained trapped fluid is concentrated under reduced pressure, and obtains drone pupae small molecule anti-oxidation peptide.This hair
It is bright that the condition for being concentrated under reduced pressure and being freeze-dried is not particularly limited, the decompression routinely used using those skilled in the art
Concentration and the condition of freeze-drying.
It is described the present invention provides the drone pupae small molecule anti-oxidation peptide that the preparation method described in such scheme is prepared
The molecular weight of drone pupae small molecule anti-oxidation peptide is 0.15~1ku.
The drone pupae small molecule anti-oxidation peptide that is prepared present invention also offers the preparation method described in such scheme or
Application of the drone pupae small molecule anti-oxidation peptide in anti-oxidation medicine and/or food is prepared described in such scheme, the drone
Pupa small molecule anti-oxidation peptide can absorb oxygen radical and remove DPPH.
It is small to a kind of drone pupae small molecule anti-oxidation peptide preparation method drone pupae provided by the invention with reference to embodiment
Molecule anti-oxidation peptide and application are described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Lyophilized drone pupae is milled, crosses 80 mesh sieves, is homogenized after adding the water of lyophilized drone pupae 15 times of volumes of weight, with dilute salt
Acid and low-concentration sodium hydroxide solution adjust pH to 6.5, add complex enzyme digest 2h under the conditions of 60 DEG C, obtain enzymolysis liquid (see
Fig. 1), complex enzyme is neutral proteinase and flavor protease, and the drone pupae powder per 1g adds the complex enzyme that total enzyme activity is 1760U,
The enzyme activity of neutral proteinase and flavor protease is than 3:1.
Enzymolysis liquid is centrifuged into 15min under conditions of 4000rpm, discards upper strata grease, takes supernatant to cross 5 μm of filter clothes, will
Filtrate carries out first step ultra-filtration and separation peptide fragment with the ultrafiltration membrane that molecular cut off is 1ku, obtains the ultrafiltration of peptide fragment molecular weight ＜ 1ku
Liquid, the molecular cut off of the ultrafiltration membrane that first step ultrafiltration uses successively are 10ku, 5ku, 3ku and 1ku, in first step ultra-filtration process
Filtrate flow velocity is 0.5~10L/H, and the membrane component pressure of the first step ultrafiltration is 0~0.8MPa, before first step ultrafiltration terminates
Add pure water to be washed, until the permeate of outflow is colourless.
The ultrafiltration membrane that the ultrafiltrate for obtaining peptide fragment molecular weight ＜ 1ku is 0.15ku with molecular cut off is carried out second step to surpass
Desalination is filtered, and, until the permeate of outflow is colourless, obtains trapped fluid with pure water for several times；The filtrate stream of second step ultrafiltration
Speed is 0.5L/H, and membrane component pressure is controlled in below 1.2Mpa.
It is freeze-dried after obtained trapped fluid is concentrated under reduced pressure, obtains drone pupae small molecule anti-oxidation peptide, yield is
41.55 ± 1.42%.
When obtained drone pupae small molecule anti-oxidation peptide is made the solution that concentration is 0.08mg/mL, small molecule is anti-oxidant
Peptide oxygen radical absorbability (ORAC) is 884.12 ± 150.47 μm of ol Trolox/g (see Fig. 2), and DPPH Scavenging activities are
83.68 ± 1.53% (see Fig. 3).
As seen from the above embodiment, a kind of preparation method of drone pupae small molecule anti-oxidation peptide provided by the invention is prepared into
The molecular weight of the drone pupae small molecule anti-oxidation peptide arrived is less than 1ku, and the antioxygenic property with high activity.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of drone pupae small molecule anti-oxidation peptide, comprises the following steps：
1) drone pupae powder is mixed with water, be homogenized, after the pH value for adjusting homogenate is 6~7, mixed with complex enzyme, 50~60
1~3h is digested at DEG C, obtains enzymolysis liquid, the complex enzyme includes endo protease and exoproteinase；
2) enzymolysis liquid that the step 1) obtains is centrifuged, obtained supernatant is filtered, obtain filtrate, institute
The speed for stating centrifugation is 4000~5000rpm, and the filtering is carried out using filter cloth, and the aperture of the filter cloth is 1~5 μm；
3) filtrate for obtaining the step 2) carries out first step ultra-filtration and separation peptide fragment, obtains the ultrafiltration of peptide fragment molecular weight ＜ 1ku
Liquid, the molecular cut off for the ultrafiltration membrane that the first step ultrafiltration uses successively is 10ku, 5ku, 3ku and 1ku；
4) ultrafiltrate for the peptide fragment molecular weight ＜ 1ku for obtaining the step 3) carries out second step ultrafiltration desalination, obtains trapped fluid,
The molecular cut off for the ultrafiltration membrane that the second step ultrafiltration uses is 0.15~0.3ku；
5) it is freeze-dried after the trapped fluid that the step 4) obtains is concentrated under reduced pressure, obtains drone pupae small molecule anti-oxidation peptide.
2. preparation method according to claim 1, it is characterised in that the total enzyme activity and drone pupae of the step 1) complex enzyme
The mass ratio of powder is (1000~2000) U:1g, the enzyme activity ratio of the endo protease and exoproteinase is (1~3):(1~
3)。
3. preparation method according to claim 1 or 2, it is characterised in that the step 1) endo protease includes alkalescence
One or more in protease, papain, trypsase and neutral proteinase.
4. preparation method according to claim 1 or 2, it is characterised in that the exoproteinase include flavor protease,
One or more in carboxypeptidase and aminopeptidase.
5. preparation method according to claim 1 or 2, it is characterised in that the granularity of step 1) the drone pupae powder is 10
~100 μm.
6. preparation method according to claim 1 or 5, it is characterised in that the quality and water of step 1) the drone pupae powder
Volume ratio be 1g:(10~15) mL.
7. preparation method according to claim 1, it is characterised in that the time that the step 2) centrifuges for 10~
15min。
8. preparation method according to claim 1, it is characterised in that the condition of the step 3) first step ultrafiltration includes：
Filtrate flow velocity is 0.5~10L/H in the first step ultra-filtration process, the membrane component pressure of the first step ultrafiltration for 0~
0.8MPa, addition pure water is washed before first step ultrafiltration terminates, until the permeate of outflow is colourless；
The condition of the second step ultrafiltration includes：The flow velocity of the second step ultrafiltration is 0.5~10L/H, the film of second step ultrafiltration
Element pressure is 0~1.2MPa, and pure water is added before second step ultrafiltration terminates and is washed, until the permeate of outflow is nothing
Color.
9. the drone pupae small molecule anti-oxidation peptide that the preparation method described in claim 1~8 any one is prepared, the hero
The molecular weight of bee pupa small molecule anti-oxidation peptide is 0.15~1ku.
10. drone pupae small molecule anti-oxidation peptide or right that the preparation method described in claim 1~8 any one is prepared
It is required that application of the drone pupae small molecule anti-oxidation peptide in anti-oxidation medicine and/or food is prepared described in 9.
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| CN108893512A (en) * | 2018-07-09 | 2018-11-27 | 青海国肽生物科技有限公司 | A kind of bee pupa protein peptides and preparation method thereof inhibited with DPP-IV with anti-fatigue effect |
| CN109055468A (en) * | 2018-08-21 | 2018-12-21 | 浙江大学 | Mulberry silkworm chrysalis anticancer active peptide BPP-1 and the preparation method and application thereof |
| CN109182428A (en) * | 2018-08-21 | 2019-01-11 | 浙江大学 | Mulberry silkworm chrysalis anticancer active peptide BPP-2 and the preparation method and application thereof |
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| CN108841905A (en) * | 2018-06-21 | 2018-11-20 | 国肽生物工程（常德）有限公司 | A kind of bee pupa protein peptides and preparation method thereof inhibited with ACE with anti-fatigue effect |
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| CN108893512B (en) * | 2018-07-09 | 2020-06-12 | 安徽国肽生物科技有限公司 | Bee pupa protein peptide with DPP-IV inhibition and anti-fatigue functions and preparation method thereof |
| CN109055468A (en) * | 2018-08-21 | 2018-12-21 | 浙江大学 | Mulberry silkworm chrysalis anticancer active peptide BPP-1 and the preparation method and application thereof |
| CN109182428A (en) * | 2018-08-21 | 2019-01-11 | 浙江大学 | Mulberry silkworm chrysalis anticancer active peptide BPP-2 and the preparation method and application thereof |
| CN109182429A (en) * | 2018-08-21 | 2019-01-11 | 浙江大学 | Mulberry silkworm chrysalis anticancer active peptide BPP-3 and the preparation method and application thereof |
| CN109182429B (en) * | 2018-08-21 | 2021-05-07 | 浙江大学 | Mulberry silkworm pupa anticancer active peptide BPP-3 and preparation method and application thereof |
| CN109609577A (en) * | 2018-12-29 | 2019-04-12 | 武汉天天好生物制品有限公司 | A kind of bee tire peptide and its preparation process and application with anti-inflammatory effect |
| CN110734902A (en) * | 2019-10-17 | 2020-01-31 | 青岛玛斯特生物技术有限公司 | complex enzyme preparation and application thereof in field of shrimp enzymolysis processing |
| CN110734902B (en) * | 2019-10-17 | 2021-08-27 | 青岛玛斯特生物技术有限公司 | Complex enzyme preparation and application thereof in field of shrimp enzymolysis processing |
| CN112195212A (en) * | 2020-10-15 | 2021-01-08 | 华中农业大学 | Method for preparing bee pupa antioxidant polypeptide by microbial fermentation |
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