CN105919071A

CN105919071A - Functional compound protein composition and preparation method thereof

Info

Publication number: CN105919071A
Application number: CN201610251441.8A
Authority: CN
Inventors: 房克敏; 赵军; 蒋彤; 蒋峰
Original assignee: BEIJING DONGFANG XINGQI FOOD PRODUCT INDUSTRY TECHN Co Ltd
Current assignee: BEIJING DONGFANG XINGQI FOOD PRODUCT INDUSTRY TECHN Co Ltd
Priority date: 2016-04-21
Filing date: 2016-04-21
Publication date: 2016-09-07

Abstract

The invention discloses a functional compound protein composition and a preparation method thereof. According to the functional compound protein composition and the preparation method thereof, soybean protein isolate, whey protein concentrate and phospholipids are utilized as main raw materials; low-temperature processing is performed on stevia rebaudiana so as to prepare a stevia rebaudiana extract to preserve active ingredients and biologically active ingredients of the stevia rebaudiana to the maximum extents; the prepared stevia rebaudiana extract is high in extraction rate, pure in flavor, natural in color and luster, and free of liquorice smell; the stevia rebaudiana extract and the main raw materials are scientifically compounded, and antifreeze polypeptides, dietary fiber powder, bee pupae enzymatic hydrolysate and wheat malt flour are added, so that the functional compound protein composition is prepared; and the prepared functional compound protein composition is appropriate in taste, good in solubility, whole in nutrition, good in biological activities and suitable for a wide range of people. The preparation method adopts a non-thermal processing technology, so that the preparation method is high in work efficiency, small in energy consumption, friendly to the environment and free of pollution; moreover, the preparation method reduces introductions of food additives and chemical additives, so that safety of the foods is effectively improved, qualities of the foods are improved and production costs are reduced. The preparation method of the functional compound protein composition realizes low-carbon production target, and is capable of realizing scale development and industrialized development.

Description

A kind of functional compound protein compositions and preparation method thereof

Technical field

The present invention relates to compound protein, particularly to a kind of functional compound protein compositions and preparation method thereof.

Background technology

Protein is the main composition material of human body, is again the main matter of human life activity.Protein adds nucleic acid, is raw The principal mode that life exists.Protein is in the meaning that ancient Latin original meaning is " primarily ", though it is an one's share of expenses for a joint undertaking of nutrient substance, But be in the status at various one center of nutrient substance, its effect mainly: 1, be used for manufacturing muscle, blood, skin and Various organs.2, health is helped to manufacture the tissue that new organization breaks down with replacement.3, to the various nutrition of cell delivery.4, adjust The balance of joint internal water.5, for immune system manufacture to antibacterium and the antibody of infection.6, help wound blood to condense and heal Close.7, manufacturing enzyme in vivo, helping food conversion is energy.Human body lacks protein can be fatal, the egg in human body The 16% of white matter about percentage of liveweight, the people of a 60kg body weight, internal have 10～11kg protein, if human body is lost internal More than the 20% of protein, vital movement will be forced out of service.The shortage symptom of common protein is that metabolic rate declines, raw Life becomes fragile and easily sick, and long-term effect infringement on organ is affirmative, the shortage of protein, the most again with the lacking of energy Weary common existence, common are upgrowth and development of children slow, weight loss.Middle-aged and elderly people be easily generated indifferent, easily enrage, emotion Instability, anemia and skinny and wizened or edema, and the secondary disease because of hypoimmunity easy infection.

Lactalbumin can resist various immunologic challenges, can promote humoral immunization and cellular immunization.The most also find that lactalbumin can be right Anti-microbial infection, such as Salmonella, streptococcal pneumonia etc..Soybean protein accounts for the 40% of soy content, is frumentum food 4～5 times of thing.Soy proteinaceous aminoacid composition is close with milk protein, and in addition to methionine is lower slightly, remaining must ammonia Base acid content is the abundantest, is vegetalitas complete protein, can be equal to animal proteinum in nutritive value.Soybean protein is to strong The positive role of Kang Suoqi mainly has: soybean protein contributes to reducing blood plasma cholesterol level, can stop urine calcium loss, help rush Enter bone health.Additionally the absorption of soybean protein has the effect promoting renal function, and soybean protein can be pacified relatively as nephrotic Full protein source.

Phospholipid is the lipids containing phospholipid root, is Life Base material.And cell membrane is just by about 4 0% protein and 50% The lipid (phospholipid is main) of left and right is constituted.It is by compositions such as lecithin, lipositol, cephalin.These phospholipid are the most right Each position and each organ of human body play corresponding function.All containing phospholipid in human body all cells, it sustains life activity Basic substance.Phospholipid, to activating cell, maintains metabolism, basal metabolism and hormonal equilibrium secretion, strengthens exempting from of human body Epidemic disease power and regeneration power, can play great effect.From the point of view of Gai Kuoing, the basic training of phospholipid is with being: strengthen mental, stable nerve, Balance endocrine, improves immunity and regeneration power, diuresis of detoxifying, blood clean, and vigorous and graceful skin keeps youth, slow down aging.

At present, prepare the more of compound protein and protein composition with soybean protein isolate, lactalbumin, phospholipid for raw material, have Main for the purpose of supplementary human nutrition: Chinese patent CN 104770743A discloses a kind of compound protein powder and preparation method thereof. Compound protein powder contains soybean protein powder, lactalbumin powder, highland barley soy Protein powder, potato protein meal, Sweet Potato Protein Powder, collagen Egg albumen powder, Semen Pisi sativi protein powder, walnut powder, meringue powder, yolk powder, soybean phospholipid, Pollen Brassicae campestris, sweeting agent, essence.Its Preparation method is that raw material is toasted, cools down, mixes, packed, finished product.Proportioning raw materials selected by the present invention meets existing For health preserving principle, scientific formulation is reasonable, has the effect of good supplementary needed by human body nutrition, and good mouthfeel, unique flavor. Chinese patent CN 105146043 A discloses a kind of Lepidinm meyenii Walp egg albumen powder, major ingredient and adjuvant are mixed with and form, and described major ingredient is Pueraria root powder and soybean protein powder；Described adjuvant, including lecithin, lactalbumin powder, fructose, vanillin, non-dairy creamer, Oligofructose, trace element, compound vitamin, silicon dioxide and Konjac glucomannan；Its dispensing mass ratio is: pueraria root powder 2.2-3.8 Part, soybean protein powder 55-80 part, lecithin 1.5-2.5 part, lactalbumin powder 4.9-8.5 part, fructose 7.3-9.2 Part, vanillin 0.0015-0.0035 part, non-dairy creamer 4.8-8.2 part, oligofructose 5.1-6.7 part, trace element 0.9-1.5 Part, compound vitamin 0.08-0.22 part, silicon dioxide 0.07-0.2 part and Konjac glucomannan 0.08-0.23 part, by dispensing powder After broken, cross 100-150 mesh sieve, mix homogeneously, to obtain final product.The present invention effectively supplements the nutrient substance lacked, and regulates nutrition comprehensively Balance, improves immunity, strong health.Chinese patent CN 103005464 B discloses a kind of lactalbumin composition, method And purposes.The invention provides multiple lactalbumin composition, it is suitable as i.e. using fluid composition as nutrient formulation, its Storage is stable and contains high-caliber intact whey protein content.

Have by prevention and treatment disease for the purpose of: Chinese patent CN 103584089 A discloses a kind of many nutritive composite proteins The processing method of powder, is made up of the raw material of following weight parts: Human-like Collagen 15, cattle colostrums 15, soybean protein 350, Lactalbumin 550, Ganoderma 5, Flos Lonicerae 5, Radix Scrophulariae 5, Radix Ophiopogonis 3, Folium Ginkgo 2, Radix phyllostachydis bambusoidis 3, Radix Salviae Miltiorrhizae 3, Rhizoma Fagopyri Dibotryis 2, Herba Lysimachiae Klattianae 3, roselle juice 30；The processing method that the invention provides a kind of many nutritive composite protein powders, its procedure of processing letter Single, raw material sources are wide, safety and Health；Use the protein powder that the inventive method produces, nutritious, instant, absorb, Utilization rate is high, can reduce the intestines and stomach and bear, and can avoid the absorption of excess fat and cholesterol, energy while supplementary protein Efficiently reduce the onset risk of cardiovascular disease.Chinese patent CN 104839736 A discloses one can prevent senile dementia The egg albumen powder of disease, it is made up of the raw material of following weight: soybean protein powder 80-90, lactalbumin powder 20-30, Flos Chrysanthemi Indici lyophilizing Powder 10-12, Semen Juglandis 10-12, Poria 12-15, Semen Sesami Nigrum 6-8, Corm Eleocharitis 12-15, Semen Nelumbinis 10-12, Fructus Jujubae 12-15, Rhizoma Dioscoreae 10-12, Flammulina velutiper (Fr.) Sing 10-12, Hericium erinaceus (Bull. Ex Fr.) Pers. 15-20, Tremella 12-15, Radix Polygalae 10-12, Thallus Laminariae (Thallus Eckloniae) 10-12, He Shou Crow 10-12, Herb Gynostemmae Pentaphylli 12-16, Folium Ginseng 2-15, Folium Ginkgo 12-15, Rhizoma Acori Graminei 10-12, nourishing additive agent 3-5.This The egg albumen powder of invention, nutritious, it is eaten for a long time and can play nourishing the brain and tranquilizing the mind, the liver and the kidney tonifying, improve the vigor of brain cell, To brain-strengthening, there is effect of senile dementia in prevention.Chinese patent discloses CN 103768585 B deer collagen albumen battalion Support compositions, be made up of deer collagen albumen, glucosamine, hyaluronic acid, soybean isoflavone and tea polyphenols.Described compositions In containing the deer collagen albumen of 70%～95% (percentage by weight), 1%～glucosamine, 1%～10% of 10% (percentage by weight) The hyaluronic acid of (percentage by weight), 0.1%～the soybean isoflavone of 5% (percentage by weight) and 0.1%～6% (weight percent Than) tea polyphenols.Described deer collagen albumen is become with Corium Cervi collagen protein groups by collagen from deer sinew, deer-bone collagenous albumen, Ligamentum cervi The weight ratio of collagen protein, deer-bone collagenous albumen and Corium Cervi collagen protein is 1:1:1.The molecular weight distribution of described deer collagen albumen Below 1500 dalton.The present invention, with deer collagen albumen as main component, is aided with glucosamine, hyaluronic acid, Semen sojae atricolor The tea polyphenols of isoflavone and antioxidation define have enhancing bone density, osteoporotic alimentation composition can be significantly improved.

Also have and mainly solve solubility problem: Chinese patent CN 103271166 A disclose a kind of soy-bean whey composite powder and Compound method.This soy-bean whey composite powder is with Non-transgenic soybean egg albumen powder and lactalbumin as primary raw material, adds Semen sojae atricolor ovum Phospholipid, soybean isoflavone, soybean oligo saccharide and other trace element, nutrition is more rich, more comprehensively, more equalizes.Containing multiple sky So active component, for high-protein nutritious food.There is the distinctive fragrance of soybean protein, smooth in taste, pure taste, strong, I.e. rush instant, supplement the nutrients the most comprehensively.In warm water somewhat stirring can rapid solution, there is intrinsic fragrance and abnormal smells from the patient, And it is the most not stratified.Chinese patent CN 102630947 B discloses the production method of a kind of low-molecular-weight nutritional protein powder, The method comprises the following steps: with soybean protein powder and lactalbumin powder as raw material, adds 70～90 DEG C of water and stirs into and mix uniformly Suspension, passes through membrane filtration: first select 5000 daltonian filter membranes under the conditions of being not less than 60 DEG C while hot, and filtrate is again by 500 Daltonian filter membrane；The filtrate that obtain is spray-dried, add spray drying powder body weight 0.5～1.5% lecithin mixing all Even.The protein powder molecular weight that present invention process prepares is little, aminoacid composition is scientific and reasonable, easily absorbs；Profile is pure white, Attractive in appearance, without bean raw meat, milk fishy smell；Soluble in water, also can comparatively fast dissolve in cold water, it is easy to dispersion, be particularly well-suited to protein Shortage crowd, weak and sickly and tumor patient.

The most single egg albumen powder of patent disclosed above or compound protein powder, either animal albumen powder or vegetable protein powder Main concentration solves the trophism of protein powder, functional (dissolubility, stability, spumescence, emulsibility, gelation etc.) With the prevention aspect such as disease, and the most more or less there is following defect: 1) containing substantial amounts of excess saturated fat, cholesterol, High heat；2) in processing technique, with the addition of substantial amounts of food additive such as pigment, sweeting agent, preservative etc.；3) dissolubility, Poor stability, reconstitutes uneven, conglomeration, lamination easily occurs；4) aminoacid composition is comprehensive not, and each nutritional labeling forms Equilibrium not；5) consumer group relates to middle-aged and elderly people and women is less, has limitation；To the bioactivity research of egg albumen powder relatively Few, the biological activity of protein powder, titer are its trophism, the comprehensive embodiment of many-sided character such as functional, in processing and storage Process of depositing is affected very big, especially in active microorganism microbial inoculum, enzyme preparation by external environment (temperature, humidity, illumination, oxygen) Manifest the most prominent etc. technical field.

For a long time, in terms of protein powder biological activity, the impact that have studied high temperature to proteins biological activity is mainly concentrated, but Ignore the impact of freezing.Antifreeze protein (Antifreeze Protein, AFP), also known as thermal hysteresis protein (Thermal Hysteresis Protein, THP), ice associated proteins (Ice Binding Proteins) or ice crystal histone (Ice Structuring Proteins).Antifreeze protein can be by being combined with specific ice crystal surface, and non-colligative property ground reduces solution Freezing point or suppression ice-crystal growth, have low-temperature protection function.Antifreeze protein can be used for the Excised Embryos of human body or animal organ, Improve its freezing quality；Can improve the freezing tolerance of specified microorganisms, when reducing Frozen food store, the formation of ice crystal and recrystallization are to food Product structural damage, improves food quality.Antifreeze protein can be applied as a kind of senior antifreezing agent, in medical science, cosmetics, life Thing and field of food all have a wide range of applications.

Processing, transport and storage science at protein powder compounds antifreeze protein (or related substances), not only can improve albumen The biological activity of matter powder, trophism, functional, and the moisture of egg albumen powder can be properly increased within the same shelf-life, fall Low production cost.The Patents document or the technical literature that solve the frost resistance of compound protein powder or compositions in prior art are less, Almost without.

Summary of the invention

Solved by the invention technical problem is that the defect overcoming existing compound protein compositions, with soybean protein isolate, condensed whey Albumen and phospholipid are primary raw material, and Folium Stevlae Rebaudianae machining at low temperature is become Stevia rebaudiana (Bertoni) Hemsl extract, compound with its science, and add freeze proof many Peptide, food fibre powder, bee pupa zymolyte, Wheat Sprout Powder, preparation is a kind of, and mouthfeel is suitable, dissolubility good, comprehensive nutrition, life Thing activity is strong, suitable population's the most functional compound protein compositions.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of functional compound protein compositions, mainly prepared by major ingredient and adjuvant by following parts by weight:

Described major ingredient is made up of the raw material of following parts by weight: soybean protein isolate 50-90 part, concentrated lactoalbumin 30-50 part, Phosphatidase 1 0-30 part；

Described adjuvant is Folium Stevlae Rebaudianae 1-3 part；

Further, described adjuvant also includes the raw material of following parts by weight: food fibre powder 20-40 part, Wheat Sprout Powder 20-40 Part, antifreeze peptide 10-20 part, bee pupa zymolyte 5-15 part；

Preferably, described functional compound protein compositions, mainly prepared by major ingredient and adjuvant by following parts by weight:

Described major ingredient is made up of the raw material of following parts by weight: soybean protein isolate 60-80 part, concentrated lactoalbumin 35-45 part, Phosphatidase 1 5-25 part；

Described adjuvant is Folium Stevlae Rebaudianae 1.5-2.5 part；

Further, described adjuvant also includes the raw material of following parts by weight: food fibre powder 25-35 part, Wheat Sprout Powder 25-35 Part, antifreeze peptide 13-17 part, bee pupa zymolyte 8-12 part；

It is highly preferred that described functional compound protein compositions, mainly prepared by major ingredient and adjuvant by following parts by weight:

Described major ingredient is made up of the raw material of following parts by weight: soybean protein isolate 70 parts, concentrated lactoalbumin 40 parts, phospholipid 20 parts,

Described adjuvant is Folium Stevlae Rebaudianae 2 parts；

Further, described adjuvant also includes the raw material of following parts by weight: food fibre powder 30 parts, Wheat Sprout Powder 30 parts, anti- Freeze polypeptide 15 parts, bee pupa zymolyte 10 parts.

Further, described food fibre powder be by dietary fiber through physics, chemical or biological method process and obtain solvable Property content of cellulose is high, biological activity strong, have important, the cellulose of positive role to human body beneficial flora, with full diet Fiber is compared, and it is functional, biological activity is more powerful；

Preferably, described food fibre powder is by one or more warps in inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber Micronizing, extruding puffing and enzyme enzymolysis and obtain；

It is highly preferred that the preparation method of described food fibre powder, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 4-6:2-4:1-3:1-2 in mass ratio uniformly mixes, micronizing to particle diameter 3-6 μm, adjusts ground product moisture and contains Amount is 17-19%, in screw speed 160-180r/min, temperature 185-200 DEG C condition extruding puffing, adds puff quality 2-4 Water again, room temperature 200-400W, 35-40KHz condition supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, arteries and veins Rush time 400-600 μ s, under the conditions of pulse frequency 200-400Hz, carry out high voltage pulse electric field processing 10-15min；Regulate with lactic acid PH value is 4.5-6.5, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution subtracts Pressure concentrates, lyophilization i.e. obtains food fibre powder；

Described enzyme be cellulase, xylanase, laccase, pectase, tannase in mass ratio 2-4:1-3:1-3:0.5-1.5:0.2-1 uniformly mixes.

Further, described Wheat Sprout Powder is with Semen Tritici aestivi as primary raw material, successively through ultrasonic cleaning, the instantaneous process of microwave, ultrasonic The techniques such as auxiliary biological enzymolysis soaks, germinates, is dried, low-temperature grinding and prepare；

Preferably, the preparation method of described Wheat Sprout Powder, comprise the steps: to be placed on equipped with 0.04-0.06% bicarbonate Semen Tritici aestivi In power 200-400W, frequency 20-30KHz room temperature ultrasonic cleaning 3-5min in the ultrasonic washing unit of sodium solution, take out, drip Dry, in frequency 2450MHz, power 3000W, temperature 38-42 DEG C, thickness of feed layer 2-4cm microwave drying 6-8s, then put temperature Degree for 33-36 DEG C, pH value be 6-8 soak in soak 4-6h, it is 0.3-0.5% that soak contains mass percent concentration Compound enzyme, ventilate once every 15-20min, ventilation pressure 0.13-0.15MPa, simultaneously in electric field intensity 10-15kV/cm, Burst length 100-300 μ s, pulse frequency 60-80Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 40-45%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20-24 DEG C, during dark germination Between be 20-24h, through germinate after wheat drying to moisture be 5-8%, low-temperature grinding, cross 80-100 mesh sieve i.e. obtain Semen Tritici aestivi Bud powder；

Described compound enzyme is that cellulase, 1,4 beta-glucanase, xylanase 2-4:1-3:1-3 in mass ratio uniformly mixes.

Further, described antifreeze peptide is the plant extract prepared for raw material with the natural plants seed containing abundant freeze proof substrate Thing is compounding with sericin peptide taken science to be formed, and is not only remarkably improved functional compound protein compositions frost resistance, and then is greatly improved it Biological activity, trophism, functional, and the moisture of functional compound protein compositions can be properly increased within the same shelf-life Content, reduces production cost；Add again dissolubility and the stability of functional compound protein compositions simultaneously, create imaginary Less than beneficial effect, be particularly suitable as the freezeproof protectant of functional compound protein compositions；

Preferably, the quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=12-29:3-9；

Preferably, described plant extract be with winter rye, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed in one or more as raw material, Through high-pressure electrostatic, high-pressure pulse electric auxiliary bigcatkin willow acid soak, refrigerating process after the most blended enzyme subsection enzymolysis and prepare；

It is highly preferred that the preparation method of described plant extract, comprise the steps: winter rye, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici Seed 9-17:2-4:1-2 in mass ratio uniformly mixes, sabot, first processes 5-7min in electric field intensity 5-7kV/cm high-pressure electrostatic； Then soaking at room temperature 2-4h in the salicylic acid solution that concentration is 10-30mg/L, simultaneously in electric field intensity 10-20kV/cm, pulse Time 150-250 μ s, carries out high voltage pulse electric field processing under the conditions of pulse frequency 150-250Hz；Rinse, drain, in 3-5 DEG C Stand 18-24h, the most successively at 1-3 DEG C of cold preservation 2-4d ,-3--5 DEG C of freezing 1-3d ,-15--18 DEG C of freezing 10-15h, put immediately In outdoor in intensity of illumination 0.5-5 ten thousand Lx natural lighting 5-7h, compound half is made to pulverize immediately after thawing, ground product grain Footpath 0.5-1.5mm, is subsequently added into the water of ground product quality 4-6 times, is 3.5-5.5 with breast acid for adjusting pH value；It is eventually adding mixing The mixed enzyme of feed liquid quality 1-2%, first in 35-50 DEG C of enzymolysis 10-30min, then in 50-60 DEG C of enzymolysis 20-40min；Enzyme Solve liquid filtration, filtrate reduced in volume to solid content is that 15-25% i.e. obtains plant extract；

Described mixed enzyme is cellulase, protease, amylase, pectase, lipase 8-10:5-7:2-4:1-3:1-2 in mass ratio Uniformly mixing.

Further, described bee pupa zymolyte with the queen bee nit of 2-3 age in days and the drone pupa of 11-12 age in days as raw material, warp Freeze proof protection, freezing crushing, vacuum outgas porn star, compound enzyme biological enzymolysis, biological demulsifying and centrifugation and obtain；

Preferably, the preparation method of described bee pupa zymolyte, comprise the steps: fresh queen bee nit and drone pupa by matter Measure more uniform mixing than 2-5:1-3, add the sericin peptide taken solution that mass percent is 4-7% and the 0.1-0.3 of mixture quality 1-2 times Solion again, stirs, and in-18--22 DEG C of freezing 12-18min, broken, percentage of damage is 98-100%, broken thing grain Footpath 200-300 μm, is 5-7 with the broken thing pH value of lactic acid regulation, is warming up to 40-50 DEG C, is added thereto to broken material amount The compound enzyme of 0.3-0.5%, mix homogeneously, in vacuum-0.07--0.09MPa degassing eliminating sargassum smell 10-15min, continues enzymolysis 10-15min obtains enzymolysis solution；Enzymolysis solution is put 5000-7000r/min traditional vacuum 8-10min in vacuum centrifuge, from upper and Lower isolated free-fat, emulsion, hydrolyzed solution and residue, be 45-55 DEG C by the emulsion obtained control temperature, add The biological demulsifying agent breakdown of emulsion 20-40min of emulsion quality 0.2-0.4%, in 4000-6000r/min traditional vacuum 2 times after breakdown of emulsion, Interval time, 1-3h, was centrifuged 10-15min every time, merges three centrifugal hydrolyzed solutions obtained, takes the egg of its quality 0.5-0.7% Clearly, put in blender and stir 10-12min in rotating speed 800-1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains Ovum Gallus domesticus album bubble Foam, adds egg white foams and merges in the hydrolyzed solution obtained, and normal pressure boils 2-4min, is down to room temperature, in 5000-7000r/min Traditional vacuum 8-10min, collects hydrolyzed solution, i.e. obtains bee pupa zymolyte through kieselguhr filtration, ultrafiltration, lyophilizing successively；

Further, described solion be containing sodium ion 15-20mg/L, zinc ion 10-15mg/L, potassium ion 8-12mg/L, Calcium ion 6-10mg/L and the aqueous solution of magnesium ion 5-8mg/L；

Further, described compound enzyme quality group becomes: flavor protease: acid protease: lipase=1-3:1-2:0.1-0.3；

Further, described traditional vacuum condition is temperature 5-8 DEG C, vacuum-0.02--0.04MPa；

Further, described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall combine one or more in class biological demulsifying agent Combination；

Preferably, the quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall combines class=3-5:1-3:1-2；

Preferably, described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl polyglucoside；

It is highly preferred that the quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=6-8:3-5.

The present invention functional compound protein compositions can be prepared via a method which:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first Folium Stevlae Rebaudianae is put into equipped with The ultrasonic washing unit of 0.1-0.3% sodium bicarbonate solution cleans 3-5min in 200-400W, 20-40KHz, drains, put micro- Ripple be dried in power 3-5kW, thickness of feed layer 2-4cm, 80-100 DEG C, be dried 2-4min, be then immersed in mass percent For 10-15min in the sericin peptide taken solution of 4-7%, take out, pulverize immediately after-18--22 DEG C of freezing 15-25min, freezing Thickness of feed layer 3-5cm, ground product particle diameter 0.3-0.5mm, it is subsequently added into the water of ground product quality 8-12 times, with breast acid for adjusting pH Value is 3.5-5.5, at room temperature in electric field intensity 25-35kV/cm, burst length 300-500 μ s, pulse frequency 200-300Hz Under the conditions of carry out high voltage pulse electric field processing 10-15min；Then under the conditions of power 150-300W, microwave exposure is carried out in room temperature Extract 8-12min, simultaneously at power 200-300W, under the conditions of frequency 30-40KHz, carry out ultrasonic assistant extraction；Add and extract The digestive enzyme of liquid quality 0.2-0.6%, in 40-50 DEG C of enzymolysis 30-50min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 2-4%, puts into stirring Machine stirs 10-12min in rotating speed 800-1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, Ovum Gallus domesticus album is steeped Foam adds in enzymolysis solution, and normal pressure boils 1-3min, is rapidly decreased to room temperature, and 80-100 eye mesh screen filters, and filtrate reduced in volume is to solid Shape thing content obtains Stevia rebaudiana (Bertoni) Hemsl extract to 8-12%；

Described digestive enzyme is that cellulase, protease, pectase 1-3:1-2:1-2 in mass ratio uniformly mixes；

2) prepared by binding agent: by the 12-16% of soybean protein isolates amount, the 5-9% of enriched whey protein amount and antifreeze peptide, The 5-15% of food fibre powder quality, the 5-15% of Wheat Sprout Powder quality are sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, uniformly mix, in merit Rate 300-500W, carries out ultrasonic dissolution 10-15min under the conditions of frequency 35-45KHz, being prepared as mass percent concentration is 14-18% Aqueous solution, as binding agent；

3) granulation, granulate: general's residue soybean protein isolate, residue concentrated lactoalbumin, phospholipid and residue food fibre powder, Residue Wheat Sprout Powder, bee pupa zymolyte uniformly mix, and obtain mixed powder, are added thereto to described binding agent, make soft material, use 10-20 mesh sieve is pelletized, and lyophilizing to moisture is 4-12%, then with the quick granulate of 10-20 mesh sieve, and mix homogeneously, obtain merit Can property compound protein compositions.

The dosage form of the present composition is oral formulations, can be powder, granule or tablet, preferably powder.Described powder Bioavailability high, it is simple to absorb and utilize, it is ensured that the using effect of product.

The present invention also provides for the preparation method of above-mentioned functions compound protein compositions, comprises the steps:

3) granulation, granulate: general's residue soybean protein isolate, residue concentrated lactoalbumin, phospholipid and residue food fibre powder, Residue Wheat Sprout Powder, bee pupa zymolyte uniformly mix, and obtain mixed powder, are added thereto to described binding agent, make soft material, use 10-20 mesh sieve is pelletized, and lyophilizing to moisture is 4-12%, then with the quick granulate of 10-20 mesh sieve, and mix homogeneously, make powder Agent, tablet or granule.

Beneficial effect:

Folium Stevlae Rebaudianae, with soybean protein isolate, concentrated lactoalbumin and phospholipid as raw material, is first used ultrasonic cleaning to kill by the present invention Worm is gone out ovum, killing microorganisms, removal pesticide residues and heavy metal ion etc., substantially increases the foodsafety of finished product；Use Microwave drying make Folium Stevlae Rebaudianae in short-term, mainly enzyme denaturing while low-temperature explosion-puffing drying, prevent extract brown stain；Containing silk The aqueous solution of glue peptide soaks rehydration, chilled and the Folium Stevlae Rebaudianae active substance that causes and cold quick property composition can be reduced to greatest extent Loss, improve the extraction ratio of Folium Stevlae Rebaudianae bioactive ingredients, meanwhile, provide good for follow-up lyophilizing, granulating process Cryoprotective agent, improves cryoprotective effects, prevents protein freeze denaturalization, improves its biological activity and titer；Omnidistance employing is low Temperature extraction process, by ultrasonic cleaning, microwave deactivating enzyme and expanded, high-pressure pulse electric extraction, ultrasonic wave added microwave extraction, biology Enzymolysis organically combines, and the extract obtained remains the natural colored of Folium Stevlae Rebaudianae, local flavor and mouthfeel to greatest extent, makes simultaneously Bioactive substance and nutraceutical agents content that extract contains maximize, and raw material extraction ratio and utilization rate are the highest, reduce Extraction cost；Use egg white foams prepared by Ovum Gallus domesticus album to enzymolysis solution flocks in short-term, eliminate the Radix Glycyrrhizae taste of stevioside further, Improve dissolubility and the stability of finished product；Part food fibre powder, Wheat Sprout Powder addition extract uniformly will be mixed with antifreeze Closing, make binding agent, embedding rate height, cohesive can be greatly prolonged well the shelf-life of finished product, the most also can improve the solid of batch mixing Thing content, reduces moisture, alleviates the burden of follow-up wet granular freezing process, shorten freeze-drying time, improve lyophilizing Efficiency, through ultrasonic dissolution, further enhancing the homogeneity of batch mixing, stability and dissolubility, and then improves the homogeneous of finished product Property, stability and dissolubility；Wet grain drying process uses vacuum lyophilization to replace existing mode of heating to be dried, and significantly carries The biological activity of the protein of high functional protein compositions and titer, prevent from heating the protein denaturation caused, the wettest Granule encapsulating layer contains the antifreeze peptide with notable cryoprotective effects using natural plant raw material to prepare, and greatly reduces further The freezing loss that lyophilizing technique causes, is effectively increased biological activity and the nutritive value of finished product, and with functional dietary fiber powder, Wheat Sprout Powder, Stevia rebaudiana (Bertoni) Hemsl extract science are compounding, significantly enhance the property balanced in nutrition of finished product and functional, dissolubility；Adopt The functional dietary fiber powder prepared by the present invention replaces existing anticaking agents, significantly improves the disintegration rate of finished product, improves Aesthetic quality after the dissolubility of finished product and dissolving, substantially prolongs the shelf-life of finished product simultaneously；Final prepare a kind of mouthfeel suitable, Dissolubility is good, comprehensive nutrition, biological activity strong, suitable population's the most functional compound protein compositions.Concrete test effect Seeing embodiment 7-10, concrete know-why is as follows:

1. the antifreeze peptide that prepared by the present invention is the plant extract prepared for raw material with the natural plants seed containing abundant freeze proof substrate Thing is compounding with sericin peptide taken scienology to be formed, and without any chemical substance and food additive, natural freeze proof substrate is comprehensive, abundant, Solubility antifreeze protein and antifreeze peptide content are high, substantially increase the foodsafety of functional compound protein compositions, not only It is remarkably improved functional compound protein compositions frost resistance, and then is greatly improved its biological activity, trophism, functional, and And the moisture of egg albumen powder can be properly increased within the same shelf-life, reduce production cost；Add again functional compound simultaneously The dissolubility of protein composition and stability, create beyond thought beneficial effect, is particularly suitable as functional compound protein The freezeproof protectant of compositions.Plant extract therein will compound containing the natural plants seed feed science of abundant freeze proof substrate, Processed by high-pressure electrostatic, high-pressure pulse electric assists Induced by Salicylic Acid, low temperature segmentation Stress treatment and natural lighting to organically combine, Make the coercing and induce down of active seed boundary's environment outside containing freeze proof substrate itself, freeze proof matrix components obtained the most comprehensively, The abundantest synthesis and accumulation, maximizing dissolution after biological enzymolysis, antifreeze peptide content can be made to increase simultaneously, improve plant The dissolubility of extract and stability, and then eliminate the problem of the finished product dissolubility difference caused because of antifreeze protein, with dietary fiber Powder, Wheat Sprout Powder, bee pupa zymolyte, phospholipid, Stevia rebaudiana (Bertoni) Hemsl extract science are compounding, better.

2. micronizing, extruding puffing, supersound extraction, high-pressure pulse electric are extracted and raw by the food fibre powder that prepared by the present invention Thing enzymolysis combination of sciences, gained food fibre powder retentiveness, dilatancy, thickening property are higher, the modified meals prepared than single method Food fiber improves 20-40%, and the impact of acid and alkali, alkali, salt, and soluble fiber cellulose content is high, than prepared by single method changing Property dietary fiber improve 10-30%, it is easier to utilized by lactic acid bacteria, improve lactic acid bacteria in the growth of human body intestinal canal and fertility, Increase kind and the quantity of probiotic bacteria flora, reduce human body intestinal canal pH value, improve human body intestinal canal microecological environment；High adsorption capacity, After modified, the specific surface area of cellulose increases, and network is enriched, and absorption affinity strengthens, and chelates, adsorbs cholesterol and bile The organic molecule ability of acids is higher, the suppression human body absorption to them；Ion-exchange capacity strengthens, to metallic element, especially It is that heavy metal element adsorption effect is higher, effectively prevent body weight for humans metal poisoning；Regulation and the resident time of maintenance intestinal microbial population, Strengthen digestion and the absorbability of intestinal, improve immunity of organisms；Effectively facilitate gastrointestinal peristalsis, slow down and eliminate flatulence, abdominal distention Deng untoward reaction；Powerful embedding effect can prevent environment (oxygen, temperature, illumination, water activity etc.) factor to functional The impact of protein composition quality, stabilizes the biological activity of functional protein compositions further, extends the shelf-life of product.

The preparation method of Wheat Sprout Powder the most of the present invention carries out ultrasonic cleaning, the instantaneous process of microwave and high-tension pulse to the Semen Tritici aestivi of immersion process Rush electric field treatment and effectively prevent soak pollution microbes, it is to avoid generation stink penetrates in Wheat Sprout Powder product, meanwhile, can Effectively strengthen corn seed coat cell wall and the relative permeability of cell membrane, improve the water absorption rate of full cereal seed, promote that seed is sprouted in advance Sending out, improve generation speed and adenosine triphosphate (ATP) content of ultra-oxygen anion free radical, stratification early stage wheat seed is multiple The activation of enzyme and release, endosperm dissolve and functional nutrient composition, bioactive ingredients, the synthesis of antioxidant content, promote Enter the respiratory metabolism of seed, accelerate nutrition and functional materials, bioactive ingredients, the enrichment of antioxidant content are entered Journey, shortens enrichment time, improves nutrition and functional materials, bioactive ingredients, the content of antioxidant content, with life Thing enzymolysis organically combines, can degrade further seed coat cellulose and hemicellulose structure, increases seed coat permeability, activates various life Active substances (endogenous enzymes etc.) vigor is the strongest, and enriching quantity is bigger, and soluble fiber cellulose content increases therewith, for Wheat Sprout Powder In Lactobacillus plantarum provide trophic factors, better significantly；By ultrasonic, microwave, high-voltage pulse electric field technology and biology Enzymolysis organically combines, and shortens soak time, improves germination percentage and germination uniformity；Wheat Sprout Powder heat can be kept to greatest extent Quick property content of material, especially antioxidant (glutathion, inositol hexaphosphate, vitamin C, polyphenol etc.), to greatest extent Ground ensures the natural colored of Wheat Sprout Powder, taste and flavor, may also function as bactericidal action simultaneously；Particularly through the instantaneous appropriateness of microwave After killing embryo, in the case of the biological activity not affecting Fructus Tritici aestivi seed, along with the absorption of moisture, wheat embryo be suppressed but not Affecting every enzyme and the growth of bioactive substance and the dissolving of endosperm, blastogenesis length is the shortest, and Repiration is weak, and sprout damage is low, Improve yield and the quality of Wheat Sprout Powder, significantly improve functional in Wheat Sprout Powder product, trophism, bioactive substance Content, the health care that improve Wheat Sprout Powder (improves body immunity, removes oxygen-derived free radicals, blood fat reducing in human body, prolongs Slow aging), nutritive value and foodsafety.The Fructus Tritici aestivi germination percentage of preparation reaches more than 97%, the long 0.2-0.5mm of bud, length Homogeneous, sprout damage is only 1.5-2.1%, reduces 4.8-7.1% than the loss of existing germination technology, and functional materials content is high, its Middle alpha-aminobutyric acid content is 303.8-315.6mg/100g, glutathion 16.1-18.3mg/100g, inositol hexaphosphate (IP₆) 460.8-484.4mg/100g, dietary fiber 3.7-4.2mg/100g.

4. the bee pupa zymolyte that prepared by the present invention with the queen bee nit of 2-3 age in days and the drone pupa of 11-12 age in days as raw material, warp Freeze proof protection, freezing crushing, vacuum outgas porn star, compound enzyme biological enzymolysis, biological demulsifying the low temperature such as separate and add with traditional vacuum Work technology and obtain, be effectively increased the utilization rate of queen bee nit and drone pupa, reduce the loss of nutrition and health substance, The bioactive ingredients remaining raw material to greatest extent becomes content of material with nutrition.Aqueous solution containing sericin peptide taken soaks, The loss of pupa worm active substance that is chilled and that cause can be reduced to greatest extent, improve the extraction ratio of pupa worm effective ingredient, with Time also provide natural freezeproof protectant for follow-up lyophilizing technique；Vacuum outgas is organically combined with compound enzyme biological enzymolysis, no Reduced by only the dissolved oxygen content of enzymolysis solution, effectively prevent bioactive ingredients oxidation deterioration, and eliminate queen bee in enzymolysis solution The worm fishy smell of larva and drone pupa, improves the mouthfeel of compound protein compositions, adds appetite, solion and queen bee pupa worm, The science of drone pupa and compound enzyme compounds so that compound enzyme vigor plays maximum, and catalytic capability is more thorough, and then makes raw material In protein and fat fully degrade, improve stability and the clarity of protein composition；By biological demulsifying agent breakdown of emulsion with true Empty centrifugal combination, can make the free-fat in enzymolysis solution and hydrolyzed solution be sufficiently separated, obtain nutrition and bioactive substance contains Amount is abundant, the hydrolyzed solution of clear, adds egg white foams and adds and boil enzyme denaturing, queen bee nit and drone pupa zymolyte more Add stable, transparent, further enhancing the dissolubility of protein composition, foam type, stability；Contain substantial amounts of queen bee simultaneously Larval polypeptides and drone pupa polypeptide, add more functional bioactive material for protein composition, further enhancing egg Functional and the trophism of white compositions.

5. the preparation method of the present invention functional compound protein compositions uses non-thermal technology technology, and work efficiency is high, and energy resource consumption is few, Environmentally friendly, pollution-free, decrease the introducing of food additive and chemical assistant, be effectively increased foodsafety, improve Product quality and reduce production cost, it is achieved that low-carbon (LC) productive target, can scale, industrialized development.

It should be noted that the result that the eating effect of this compound protein compositions is that each component is mutually collaborative, interacts, not The superposition of simple raw material function, the science of each raw material components is compounding and extracts, and the effect of generation is considerably beyond each single component merit The most advanced and practicality can be had with the superposition of effect.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is this Method well known to skilled person.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, The spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, real without departing substantially from the present invention On the premise of matter and scope, the various changes or the change that carry out the material component in these embodiments and consumption fall within this Bright protection domain.

Prepared by embodiment 1 raw material

1. the preparation of food fibre powder:

The preparation method of described food fibre powder, comprises the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 5:3:2:1.5 in mass ratio uniformly mixes, micronizing to particle diameter 5 μm, and adjusting ground product moisture is 18%, in screw rod Rotating speed 170r/min, 192 DEG C of condition extruding puffings of temperature, add the water of puff quality 3 times, room temperature 300W, 40KHz bar Part supersound extraction 12min, then in electric field intensity 30kV/cm, burst length 500 μ s, carry out height under the conditions of pulse frequency 300Hz Pressure impulse electric field processes 12min；It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, in 50 DEG C of enzymes Solve 35min；Enzymolysis solution concentrating under reduced pressure, lyophilization i.e. obtain food fibre powder；

Described enzyme is that cellulase, xylanase, laccase, pectase, tannase 3:2:2:1:0.6 in mass ratio is uniform Mixing.

2. the preparation of Wheat Sprout Powder:

The preparation method of described Wheat Sprout Powder, comprises the steps: to be placed on Semen Tritici aestivi the ultrasound wave equipped with 0.05% sodium bicarbonate solution In power 300W, frequency 25KHz room temperature ultrasonic cleaning 4min in cleaning machine, take out, drain, in frequency 2450MHz, power 3000W, temperature 40 DEG C, thickness of feed layer 3cm microwave drying 7s, then put temperature be 35 DEG C, pH value be 7 soak in soak Bubble 5h, soak contains the compound enzyme that mass percent concentration is 0.4%, ventilates once every 18min, ventilation pressure 0.14MPa, Simultaneously in electric field intensity 12kV/cm, burst length 200 μ s, pulse frequency 70Hz condition carries out high voltage pulse electric field processing, until Wheat water content content is 42%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 22 DEG C, The dark germination time is 22h, and wheat drying to the moisture after germinateing is 6%, low-temperature grinding, crosses 90 mesh sieves and i.e. obtains Semen Tritici aestivi Bud powder；

Described compound enzyme is that cellulase, 1,4 beta-glucanase, xylanase 3:2:2 in mass ratio uniformly mixes.

3. the preparation of plant extract:

The preparation method of described plant extract, comprise the steps: by winter rye, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed by quality More uniform mixing than 13:3:1.5, sabot, first process 6min in electric field intensity 6kV/cm high-pressure electrostatic；Then in concentration it is Soaking at room temperature 3h in the salicylic acid solution of 20mg/L, simultaneously in electric field intensity 15kV/cm, burst length 200 μ s, pulse frequency High voltage pulse electric field processing is carried out under the conditions of 200Hz；Rinse, drain, stand 21h in 4 DEG C, the most successively at 2 DEG C of cold preservation 3d, -4 DEG C of freezing 2d ,-16 DEG C of freezing 12h, be immediately placed at outdoor in intensity of illumination 20,000 Lx natural lighting 6h, makes compound half thaw After pulverize immediately, ground product particle diameter 1.0mm, be subsequently added into the water of ground product quality 5 times, with breast acid for adjusting pH value be 4.5； It is eventually adding the mixed enzyme of mixed liquor quality 1.5%, first in 42 DEG C of enzymolysis 20min, then in 55 DEG C of enzymolysis 30min；Enzyme Solve that liquid filters, filtrate reduced in volume to solid content is 20% i.e. to obtain plant extract；

Described mixed enzyme is that cellulase, protease, amylase, pectase, lipase 9:6:3:2:1.5 in mass ratio is uniform Mixing.

4. the preparation of bee pupa zymolyte:

The preparation method of described bee pupa zymolyte, comprises the steps: fresh queen bee nit and drone pupa 3:2 in mass ratio Uniformly mixing, the sericin peptide taken solution that mass percent is 6% of addition mixture quality 1.5 times and the solion of 0.2 times, stir Mixing uniformly, in-20 DEG C of freezing 15min, broken, percentage of damage is 99%, broken thing particle diameter 250 μm, with the broken thing of lactic acid regulation PH value is 6, is warming up to 45 DEG C, is added thereto to the compound enzyme of broken material amount 0.4%, and mix homogeneously, in vacuum-0.08MPa Degassing eliminating sargassum smell 12min, continues enzymolysis 12min and obtains enzymolysis solution；Enzymolysis solution is put in vacuum centrifuge 6000r/min vacuum from Heart 9min, from top to bottom isolated free-fat, emulsion, hydrolyzed solution and residue, by the emulsion obtained control temperature be 50 DEG C, add the biological demulsifying agent breakdown of emulsion 30min of emulsion quality 0.3%, in 5000r/min traditional vacuum 2 times after breakdown of emulsion, Interval time, 2h, was centrifuged 12min every time, merges three centrifugal hydrolyzed solutions obtained, takes the Ovum Gallus domesticus album of its quality 0.6%, put into and stir Mix in machine and stir 11min in rotating speed 900r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, egg white foams is added Merging in the hydrolyzed solution obtained, normal pressure boils 3min, is down to room temperature, in 6000r/min traditional vacuum 9min, collects hydrolyzed solution, Bee pupa zymolyte is i.e. obtained successively through kieselguhr filtration, ultrafiltration, lyophilizing；

Described solion be containing sodium ion 18mg/L, zinc ion 12mg/L, potassium ion 10mg/L, calcium ion 8mg/L and The aqueous solution of magnesium ion 6mg/L；

Described compound enzyme quality group becomes: flavor protease: acid protease: lipase=2:1.5:0.2；

Described traditional vacuum condition is temperature 6 DEG C, vacuum-0.03MPa；

The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall combines class=4:2:1.5；

The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=7:4.

Food fibre powder that example 3 below-5 is used, Wheat Sprout Powder, plant extract, bee pupa zymolyte are embodiment 1 preparation.

Embodiment 2

Described major ingredient is made up of the raw material of following parts by weight: soybean protein isolate 50 parts, concentrated lactoalbumin 30 parts, phospholipid 10 parts；

Described adjuvant is Folium Stevlae Rebaudianae 1 part；

Preparation method, comprises the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.1% In the ultrasonic washing unit of sodium bicarbonate solution in 200W, 20KHz clean 3min, drain, put in microwave drying in power 3kW, Thickness of feed layer 2cm, 80 DEG C, dry 2min, be then immersed in 10min in the sericin peptide taken solution that mass percent is 4%, take out, Pulverize immediately after-18 DEG C of freezing 15min, freezing thickness of feed layer 3cm, ground product particle diameter 0.3mm, be subsequently added into ground product The water that quality is 8 times, is 3.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 25kV/cm, and burst length 300 μ s, arteries and veins High voltage pulse electric field processing 10min is carried out under the conditions of rushing frequency 200Hz；Then under the conditions of power 150W, microwave is carried out in room temperature Irradiation extracts 8min, simultaneously at power 200W, carries out ultrasonic assistant extraction under the conditions of frequency 30KHz；Add extracting solution quality The digestive enzyme of 0.2%, in 40 DEG C of enzymolysis 30min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 2%, puts in blender in rotating speed 800r/min Stirring 10min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, adds egg white foams in enzymolysis solution, and normal pressure boils 1min, Being rapidly decreased to room temperature, 80 eye mesh screens filter, and filtrate reduced in volume to solid content to 8% obtains Stevia rebaudiana (Bertoni) Hemsl extract；

Described digestive enzyme is that cellulase, protease, pectase 1:1:1 in mass ratio uniformly mixes；

2) prepared by binding agent: be sequentially added into Folium Stevlae Rebaudianae by the 14% of soybean protein isolates amount, the 7% of enriched whey protein amount In extract, uniformly mix, at power 300W, carry out ultrasonic dissolution 10min under the conditions of frequency 35KHz, be prepared as percent mass Specific concentration is the aqueous solution of 14%, as binding agent；

3) granulation, granulate: residue soybean protein isolate, residue concentrated lactoalbumin, phospholipid are uniformly mixed, obtains mixed powder, Being added thereto to described binding agent, make soft material, pelletize with 10 mesh sieves, lyophilizing to moisture is 4%, then uses 10 mesh sieves Quickly granulate, mix homogeneously, make powder.

Embodiment 3

Described adjuvant is Folium Stevlae Rebaudianae 2 parts, food fibre powder 30 parts, Wheat Sprout Powder 30 parts, antifreeze peptide 15 parts, bee pupa enzyme Solve thing 10 parts.

Preparation method, comprises the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.2% In the ultrasonic washing unit of sodium bicarbonate solution in 300W, 30KHz clean 4min, drain, put in microwave drying in power 4kW, Thickness of feed layer 4cm, 90 DEG C, dry 3min, be then immersed in 12min in the sericin peptide taken solution that mass percent is 6%, take out, Pulverize immediately after-20 DEG C of freezing 20min, freezing thickness of feed layer 4cm, ground product particle diameter 0.4mm, be subsequently added into ground product The water that quality is 10 times, is 4.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 30kV/cm, and burst length 400 μ s, High voltage pulse electric field processing 12min is carried out under the conditions of pulse frequency 250Hz；Then carry out micro-under the conditions of power 200W in room temperature Amplitude, according to extracting 10min, simultaneously at power 250W, carries out ultrasonic assistant extraction under the conditions of frequency 35KHz；Add extracting solution The digestive enzyme of quality 0.4%, in 45 DEG C of enzymolysis 40min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 3%, puts in blender in rotating speed 900 R/min stirs 11min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, is added by egg white foams in enzymolysis solution, and normal pressure boils Boiling 2min, is rapidly decreased to room temperature, and 90 eye mesh screens filter, and filtrate reduced in volume to solid content to 10% obtains Stevia rebaudiana (Bertoni) Hemsl extract；

Described digestive enzyme is that cellulase, protease, pectase 2:1.5:1.5 in mass ratio uniformly mixes；

2) prepared by binding agent: by the 14% of soybean protein isolates amount, the 6% of enriched whey protein amount, dietary fiber opaque Amount 10%, the 10% of Wheat Sprout Powder quality, antifreeze peptide be sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, uniformly mix, at power 400W, Carry out ultrasonic dissolution 12min under the conditions of frequency 40KHz, be prepared as the aqueous solution that mass percent concentration is 16%, as binding agent；

3) pelletize, granulate: will residue soybean protein isolate, residue concentrated lactoalbumin, phospholipid, residue food fibre powder, Residue Wheat Sprout Powder, bee pupa zymolyte uniformly mix, and obtain mixed powder, are added thereto to described binding agent, make soft material, use 15 mesh sieves are pelletized, and lyophilizing to moisture is 8%, then with the 15 quick granulate of mesh sieve, mix homogeneously, makes powder；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=20:6.

Embodiment 4

Described major ingredient is made up of the raw material of following parts by weight: soybean protein isolate 60 parts, concentrated lactoalbumin 35 parts, phospholipid 15 parts；

Described adjuvant is Folium Stevlae Rebaudianae 1.5 parts, food fibre powder 25 parts, Wheat Sprout Powder 25 parts, antifreeze peptide 13 parts, bee pupa Zymolyte 8 parts；

Preparation method, comprises the steps:

2) prepared by binding agent: by the 12% of soybean protein isolates amount, the 5% of enriched whey protein amount, dietary fiber opaque Amount 5%, the 5% of Wheat Sprout Powder quality, antifreeze peptide be sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, uniformly mix, at power 300W, Carry out ultrasonic dissolution 10min under the conditions of frequency 35KHz, be prepared as the aqueous solution that mass percent concentration is 14%, as binding agent；

3) pelletize, granulate: will residue soybean protein isolate, residue concentrated lactoalbumin, phospholipid, residue food fibre powder, Residue Wheat Sprout Powder, bee pupa zymolyte uniformly mix, and obtain mixed powder, are added thereto to described binding agent, make soft material, use 10 mesh sieves are pelletized, and lyophilizing to moisture is 4%, then with the 10 quick granulate of mesh sieve, mix homogeneously, makes powder；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=12:3.

Embodiment 5

Described major ingredient is made up of the raw material of following parts by weight: soybean protein isolate 80 parts, concentrated lactoalbumin 45 parts, phospholipid 25 parts；

Described adjuvant is Folium Stevlae Rebaudianae 2.5 parts, food fibre powder 35 parts, Wheat Sprout Powder 35 parts, antifreeze peptide 17 parts, bee pupa Zymolyte 12 parts；

Preparation method, comprises the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.3% In the ultrasonic washing unit of sodium bicarbonate solution in 400W, 40KHz clean 5min, drain, put in microwave drying in power 5kW, Thickness of feed layer 4cm, 100 DEG C, dry 4min, be then immersed in 15min in the sericin peptide taken solution that mass percent is 7%, take out, Pulverize immediately after-22 DEG C of freezing 25min, freezing thickness of feed layer 5cm, ground product particle diameter 0.5mm, be subsequently added into ground product The water that quality is 2 times, is 5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 35kV/cm, and burst length 500 μ s, arteries and veins High voltage pulse electric field processing 15min is carried out under the conditions of rushing frequency 300Hz；Then under the conditions of power 300W, microwave is carried out in room temperature Irradiation extracts 12min, simultaneously at power 300W, carries out ultrasonic assistant extraction under the conditions of frequency 40KHz；Add extracting solution matter The digestive enzyme of amount 0.6%, in 50 DEG C of enzymolysis 50min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 4%, puts in blender in rotating speed 1000 R/min stirs 12min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, is added by egg white foams in enzymolysis solution, and normal pressure boils Boiling 3min, is rapidly decreased to room temperature, and 100 eye mesh screens filter, and filtrate reduced in volume to solid content to 12% obtains Stevia rebaudiana (Bertoni) Hemsl extract；

Described digestive enzyme is that cellulase, protease, pectase 3:2:2 in mass ratio uniformly mixes；

2) prepared by binding agent: by the 16% of soybean protein isolates amount, the 9% of enriched whey protein amount, dietary fiber opaque Amount 15%, the 15% of Wheat Sprout Powder quality, antifreeze peptide be sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, uniformly mix, at power 500W, Carry out ultrasonic dissolution 15min under the conditions of frequency 45KHz, be prepared as the aqueous solution that mass percent concentration is 18%, as binding agent；

3) pelletize, granulate: will residue soybean protein isolate, residue concentrated lactoalbumin, phospholipid, residue food fibre powder, Residue Wheat Sprout Powder, bee pupa zymolyte uniformly mix, and obtain mixed powder, are added thereto to described binding agent, make soft material, use 20 mesh sieves are pelletized, and lyophilizing to moisture is 12%, then with the 20 quick granulate of mesh sieve, mix homogeneously, makes tablet；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=29:9.

Embodiment 6

Described major ingredient is made up of the raw material of following parts by weight: soybean protein isolate 90 parts, concentrated lactoalbumin 50 parts, phospholipid 30 parts；

Described adjuvant is Folium Stevlae Rebaudianae 3 parts, food fibre powder 40 parts, Wheat Sprout Powder 40 parts, antifreeze peptide 20 parts, bee pupa enzyme Solve thing 15 parts；

Preparation method, comprises the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.1% In the ultrasonic washing unit of sodium bicarbonate solution in 400W, 20KHz clean 5min, drain, put in microwave drying in power 3kW, Thickness of feed layer 2cm, 100 DEG C, dry 2min, be then immersed in 10min in the sericin peptide taken solution that mass percent is 7%, take out, Pulverize immediately after-22 DEG C of freezing 15min, freezing thickness of feed layer 5cm, ground product particle diameter 0.3mm, be subsequently added into ground product The water that quality is 12 times, is 3.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 35kV/cm, and burst length 300 μ s, High voltage pulse electric field processing 10min is carried out under the conditions of pulse frequency 300Hz；Then carry out micro-under the conditions of power 300W in room temperature Amplitude, according to extracting 8min, simultaneously at power 300W, carries out ultrasonic assistant extraction under the conditions of frequency 30KHz；Add extracting solution matter The digestive enzyme of amount 0.6%, in 40 DEG C of enzymolysis 50min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 2%, puts in blender in rotating speed 1000 R/min stirs 10min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, is added by egg white foams in enzymolysis solution, and normal pressure boils Boiling 3min, is rapidly decreased to room temperature, and 80 eye mesh screens filter, and filtrate reduced in volume to solid content to 12% obtains Stevia rebaudiana (Bertoni) Hemsl extract；

Described digestive enzyme is that cellulase, protease, pectase 1:2:1 in mass ratio uniformly mixes；

2) prepared by binding agent: by the 12% of soybean protein isolates amount, the 9% of enriched whey protein amount, dietary fiber opaque Amount 5%, the 15% of Wheat Sprout Powder quality, antifreeze peptide be sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, uniformly mix, at power 300W, Carry out ultrasonic dissolution 10min under the conditions of frequency 45KHz, be prepared as the aqueous solution that mass percent concentration is 18%, as binding agent；

3) pelletize, granulate: will residue soybean protein isolate, residue concentrated lactoalbumin, phospholipid, residue food fibre powder, Residue Wheat Sprout Powder, bee pupa zymolyte uniformly mix, and obtain mixed powder, are added thereto to described binding agent, make soft material, use 10 mesh sieves are pelletized, and lyophilizing to moisture is 12%, then with the 10 quick granulate of mesh sieve, mix homogeneously, makes granule；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=12:9.

The sensory evaluation test of embodiment 7 present invention functional compound protein compositions

Invite functional compound protein powder and commercially available two kinds of similar identical dates of manufacture that the embodiment of the present invention 3 prepared by 24 personnel Compound protein powder judge, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, the middle age, Old each 4, men and women half and half, and layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes Outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), marking personnel are independently carried out, It is independent of each other, judges result with guarantee accurate.Being added up judging result, equal score value takes approximation, retains integer, tool Body is shown in Table 1:

Table 1: sensory evaluation statistical result

Note: show significant difference (P ＜ 0.05) with a line internal standard difference lowercase alphabet, the different capitalization of mark represents that difference is extremely notable (P ＜ 0.01), Indicate same letter and represent that difference is not notable (P ＞ 0.05).

Result above shows, functional compound protein powder prepared by the present invention from outward appearance, quality, local flavor and mouthfeel any one all It is substantially better than commercially available lyophilized powder, particularly outward appearance, local flavor and mouthfeel fabulous, remains the natural of raw-food material to greatest extent Color and luster, taste and flavor, also be adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.

It should be understood that functional compound protein compositions prepared by embodiment of the present invention 4-6 has above-mentioned experiment effect equally, Between each embodiment and little with above-mentioned experiment effect diversity.

The biological activity test of embodiment 8 present invention functional compound protein compositions

The powdery saccharifying enzyme of functional compound protein powder prepared by the embodiment of the present invention 3 and commercially available identical date of manufacture respectively at Store 12 months under the conditions of-12 DEG C and 40 DEG C, use detection side in " GB8726-2006 food additive saccharidase preparation " Method measures egg albumen powder and the enzyme activity of powder powdery saccharifying enzyme, calculates enzyme loss rate alive, and enzyme loss rate alive refers to that actually detected enzyme is lived Power and product mark the difference of enzyme activity and account for the percentage rate marking enzyme activity, result such as table 2

Table 2 stores phase enzyme activity loss rate

Result above shows, stores 12 months under the conditions of 0 DEG C and 40 DEG C, and the functional compound protein powder of the present invention is than commercially available powdery Glucoamylase enzyme is lived in losing and is reduced by 34% and 10% respectively, has excellent temperature storage, and enzyme activity is highly stable, also illustrates simultaneously The functional compound protein of the present invention divide containing the loss rate of other bioactive substance extremely low, there is the most freeze proof, heat resistance Can, long shelf-life.

Embodiment 9 present invention functional compound protein compositions dissolubility test

The powder that egg albumen powder is prepared as a kind of raw-food material, with reference to the stability criterion of solid beverage, by dissolution time, dissolving Rear homogeneity is defined as the quality stability index of egg albumen powder, and it the most directly reflects the dissolution degree of product, also reflects product Product inherent quality and stability, be the most important quality control index of egg albumen powder.

Dissolution time: 1:5 in mass ratio adds 55-65 DEG C of warm water, is sufficiently stirred for, the time being completely dissolved；

Homogeneity characterizing method: the most homogeneous, nothing layering 90-100 divides；Sheaf of differentials 80-89 divides；Layering 70-79 divides；Relatively divide Layer 0-69 divides；Severe delamination 50-59 divides；Precipitate less than 49 points (granule is the biggest, quantity is the most for precipitation, and mark is the lowest).

Functional compound protein powder prepared by the embodiment of the present invention 3 and commercially available same type, same specification, being combined with the date of manufacture After egg albumen powder dissolves in glass, standing, observe homogeneity, every 5min observed and recorded result once, the results are shown in Table 3:

Table 3: lyophilized powder clarity testing result after dissolving

Result above shows: compound protein powder of the present invention is compared with commercially available compound protein powder, and dissolubility is preferable, and dissolution time is short, Stability of solution after dissolving is strong, and homogeneity is strong, and presentation quality is preferable, along with the prolongation of time of repose, commercially available compound protein powder Solution liquid phase is relatively unstable, produces precipitation, leverages presentation quality and the inherent quality of beverage, this is also described simultaneously Bright compound protein powder inherent quality is preferable, and eating effect is safe and stable.

The impact on immunity of organisms of the embodiment 10 present invention functional compound protein compositions

1 experiment purpose

By exercise tolerance test (mouse forced swimming), checking the present invention functional compound protein compositions raising immunity, Antifatigue effect.

2 experiment materials and reagent

2.1 for reagent thing:

Commercially available functional compound protein compositions (G1)；Commercially available functional compound protein compositions (G2)；The embodiment of the present invention Functional compound protein compositions (G3-G7) prepared by 2-6.

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), the limited public affairs of Nanjing chemical reagent Department；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. laboratory animal

ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, little during experiment The free diet of Mus.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof is public Department；Water-bath: DK-S26 type, upper Nereid grand experimental facilities company limited；Electronic scale: BS224S type, Sartorius Company；Stopwatch, thermometer

5. experiment packet

5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, and often group 10, the 1st group to the 7th component Not Gei the medicine of G1～G7, the 8th group is blank group, gives isopyknic distilled water, the often every average daily gavage of group 1 time, Gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations the 1st group are to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank Group: distilled water 150ml.

6. experimental technique

6.1 swimmings with a load attached to the body experiment lasts are administered after 30min, put mice in swimming trunk, and the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, record mice swimming starts to the dead time, when swimming as mice Between.

After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, Pluck after rest 60min eyeball blood sampling 0.5mL (being not added with anticoagulant), put 4 DEG C of refrigerator 3h, after hemopexis 2000r/min from Heart 15min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.

After the mensuration last of 6.3 hepatic glycogen is administered 30min, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical vertebra Mice is put to death in dislocation, cleans with normal saline, and with after filter paper suck dry moisture, accurately weighs liver 100mg, and hepatic glycogen detects Test kit detection Mouse Liver glycogen content.

The mensuration last of 6.4 blood lactase acid is taken a blood sample after being administered 30min, does not the most bear a heavy burden at the water went swimming 10min that temperature is 30 DEG C Rear stopping.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add after rest 20min after swimming, after swimming Entering in 40 μ L rupture of membranes liquid, the most fully vibration smudge cells lactic acid instrument measures.(blood lactase acid area under curve=5 × (swimming The blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min after front blood lactase acid value+3 × swimming)

7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value

8. statistical method experimental data is usedRepresent, use t inspection to compare between organizing

9. experimental result

The impact on Mouse Weight of the 9.1 present invention functional compound protein compositions

Each group mice after giving G1～G9 medicine, front, in, shown in post-weight see table respectively, at the beginning of each group mice Initial body weight and weightening finish body weight compare equal no difference of science of statistics (P ＞ 0.05) with matched group, show that G1～G9 medicine is all without significantly Toxicity.Experimental result refers to table 4.

The original body mass of table 4 swimming with a load attached to the body experiment mice, body weight in mid-term and end body weight

The impact on the mice burden swimming time of the 9.2 present invention functional compound protein compositions

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, can be obviously prolonged mice and bear Weight swimming time, has significant difference (P ＜ 0.05), the present invention functional compound protein compositions G3～G7 medicine with empty White matched group compares, and can significantly extend the mice burden swimming time, have pole significant difference (P ＜ 0.01), and the most excellent In G1～G2 medicine.The results detailed in Table 5.

The impact on the mice burden swimming time of the table 5 functional compound protein compositions

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

9.3 present invention functional compound protein compositions is on the impact of blood lactase acid before and after mouse movement

After per os gives the functional compound protein compositions of the mice present invention, the present invention functional compound protein compositions G3～ Blood lactase acid area under curve after mouse movement is compared by G7 medicine with matched group significant difference (P ＜ 0.05), G1～G2 Decrease though medicine group Mouse Blood lactic acid area under curve compares with matched group, but and no difference of science of statistics (P ＞ 0.05). The results are shown in Table 6.

Table 6 present invention functional compound protein compositions is on the impact of blood lactase acid level before and after mouse movement

" * " p < 0.05vs blank；

The impact on Mouse Liver glycogen of the 9.4 present invention functional compound protein compositions

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, and Mouse Liver glycogen content all has bright Aobvious rising, have significant difference (P ＜ 0.05), the present invention functional compound protein compositions G3～G7 medicine with Blank group compares, and Mouse Liver glycogen content all has significantly rising, has pole significant difference (P ＜ 0.01), and bright Show and be better than G1～G2 medicine.The results detailed in Table 7.

The impact on Mouse Liver glycogen content of the table 7 present invention functional compound protein compositions

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

The impact on mice serum carbamide of the 9.5 present invention functional compound protein compositions

After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, serum urea after mouse movement Content all has significantly reduction, has significant difference (P ＜ 0.05), the present invention functional compound protein compositions G3～G7 Medicine compares with blank group, and after mouse movement, serum urea content all has significantly reduction, has pole significant difference (P ＜ 0.01), and it is substantially better than G1～G2 medicine.The results detailed in Table 8.

The impact on mice serum urea content of the table 8 present invention functional compound protein compositions

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

10. experiment conclusion

This experiment is mainly tested by mice burden swimming, and to observe the present invention functional compound for the deposit of detection Mouse Liver glycogen simultaneously Protein composition improves immunity, the effect of resisting fatigue.Preliminary Results shows as follows:

1, G3～G7 of the present invention functional compound protein compositions all can extend the mice burden swimming time (P ＜ 0.01), and Effect is substantially better than the functional compound protein compositions of other G1～G2.

2, biochemistry detection aspect shows, after G3～G7 of the present invention functional compound protein compositions each dosage group all can reduce motion Lactic acid content produced by glucose anerobic glycolysis in mice serum, compares with matched group and has significant difference (P ＜ 0.05), Although and the functional compound protein compositions of other G1～G2 also can reduce after motion glucose anerobic glycolysis institute in mice serum The lactic acid content produced, but compare with matched group, no difference of science of statistics (P ＞ 0.05)；

3, G3～G7 of the present invention functional compound protein compositions each dosage group all can significantly improve the deposit of glycogen in mouse liver (P ＜ 0.01), and effect is substantially better than the functional compound protein compositions of other G1～G2；

4, metabolic arthritis model finds, G3～G7 of the present invention functional compound protein compositions can significantly reduce serum after mice swimming The content (P ＜ 0.01) of middle carbamide, and effect is substantially better than other G1～G2 functional compound protein compositions；

11. conclusions

Above-mentioned experiment proves that the present invention functional compound protein compositions can significantly improve immunity of organisms, improves the muscle power of mice and resistance to Power, reduces urea in serum and the content of lactic acid after mouse movement, and can significantly improve the deposit of glycogen in mouse liver, help In alleviating the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.

Claims

1. a functional compound protein compositions, mainly prepared by major ingredient and adjuvant by following parts by weight: described major ingredient is by following heavy The raw material composition of amount number: soybean protein isolate 50-90 part, concentrated lactoalbumin 30-50 part, phosphatidase 1 0-30 part；Described auxiliary Material is Folium Stevlae Rebaudianae 1-3 part.

Functional compound protein compositions the most as claimed in claim 1, it is characterised in that described adjuvant also includes following parts by weight Raw material: food fibre powder 20-40 part, Wheat Sprout Powder 20-40 part, antifreeze peptide 10-20 part, bee pupa zymolyte 5-15 Part.

Functional compound protein compositions the most as claimed in claim 2, it is characterised in that the preparation method of described food fibre powder, Comprise the following steps: inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 4-6:2-4:1-3:1-2 in mass ratio is uniformly mixed Close, micronizing to particle diameter 3-6 μm, adjust ground product moisture be 17-19%, in screw speed 160-180r/min, Temperature 185-200 DEG C condition extruding puffing, adds the water of puff quality 2-4 times, room temperature 200-400W, 35-40KHz condition Supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, burst length 400-600 μ s, pulse frequency 200-400Hz Under the conditions of carry out high voltage pulse electric field processing 10-15min；It is 4.5-6.5 with breast acid for adjusting pH value, adds mixture quality The enzyme of 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution concentrating under reduced pressure, lyophilization i.e. obtain food fibre powder；

Functional compound protein compositions the most as claimed in claim 2, it is characterised in that the preparation method of described Wheat Sprout Powder, bag Include following steps: Semen Tritici aestivi is placed in the ultrasonic washing unit equipped with 0.04-0.06% sodium bicarbonate solution in power 200-400W, Frequency 20-30KHz room temperature ultrasonic cleaning 3-5min, takes out, drains, in frequency 2450MHz, power 3000W, temperature 38-42 DEG C, Thickness of feed layer 2-4cm microwave drying 6-8s, then put temperature be 33-36 DEG C, pH value be 6-8 soak in soak 4-6h, Soak contains the compound enzyme that mass percent concentration is 0.3-0.5%, ventilates once every 15-20min, ventilation pressure 0.13-0.15MPa, simultaneously in electric field intensity 10-15kV/cm, burst length 100-300 μ s, pulse frequency 60-80Hz condition is entered Horizontal high voltage impulse electric field processes, until wheat water content content is 40-45%；Semen Tritici aestivi after immersion drains, and carries out dark in growing floor Germinateing, dark germination temperature keeps 20-24 DEG C, and the dark germination time is 20-24h, and the wheat drying after germinateing to moisture is 5-8%, low-temperature grinding, cross 80-100 mesh sieve and i.e. obtain Wheat Sprout Powder；

Functional compound protein compositions the most as claimed in claim 2, it is characterised in that the quality group of described antifreeze peptide becomes: Plant extract: sericin peptide taken=12-29:3-9；

The preparation method of described plant extract, comprise the steps: by winter rye, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed by quality More uniform mixing than 9-17:2-4:1-2, sabot, first process 5-7min in electric field intensity 5-7kV/cm high-pressure electrostatic；Then exist Concentration be 10-30mg/L salicylic acid solution in soaking at room temperature 2-4h, simultaneously in electric field intensity 10-20kV/cm, burst length 150-250 μ s, carries out high voltage pulse electric field processing under the conditions of pulse frequency 150-250Hz；Rinse, drain, in 3-5 DEG C of standing 18-24h, the most successively at 1-3 DEG C of cold preservation 2-4d ,-3--5 DEG C of freezing 1-3d ,-15--18 DEG C of freezing 10-15h, it is immediately placed at room Outer in intensity of illumination 0.5-5 ten thousand Lx natural lighting 5-7h, make compound half pulverize immediately after thawing, ground product particle diameter 0.5-1.5mm, is subsequently added into the water of ground product quality 4-6 times, is 3.5-5.5 with breast acid for adjusting pH value；It is eventually adding compound The mixed enzyme of liquid quality 1-2%, first in 35-50 DEG C of enzymolysis 10-30min, then in 50-60 DEG C of enzymolysis 20-40min；Enzymolysis Liquid filters, filtrate reduced in volume to solid content is that 15-25% i.e. obtains plant extract；

Functional compound protein compositions the most as claimed in claim 2, it is characterised in that the preparation method of described bee pupa zymolyte, Comprise the steps: uniformly to mix fresh queen bee nit with drone pupa 2-5:1-3 in mass ratio, add mixture quality 1-2 The sericin peptide taken solution that mass percent is 4-7% again and the solion of 0.1-0.3 times, stir, cold in-18--22 DEG C Freezing 12-18min, broken, percentage of damage is 98-100%, broken thing particle diameter 200-300 μm, with the broken thing pH value of lactic acid regulation For 5-7, being warming up to 40-50 DEG C, be added thereto to the compound enzyme of broken material amount 0.3-0.5%, mix homogeneously, in vacuum -0.07--0.09MPa deaerates eliminating sargassum smell 10-15min, continues enzymolysis 10-15min and obtains enzymolysis solution；Enzymolysis solution is put traditional vacuum 5000-7000r/min traditional vacuum 8-10min in machine, from top to bottom isolated free-fat, emulsion, hydrolyzed solution and residual Slag, is 45-55 DEG C by the emulsion obtained control temperature, adds the biological demulsifying agent breakdown of emulsion of emulsion quality 0.2-0.4% 20-40min, in 4000-6000r/min traditional vacuum 2 times after breakdown of emulsion, interval time, 1-3h, was centrifuged 10-15min every time, closed And three centrifugal hydrolyzed solutions obtained, take the Ovum Gallus domesticus album of its quality 0.5-0.7%, put in blender in rotating speed 800-1000r/min Stirring 10-12min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, adds egg white foams and merges in the hydrolyzed solution obtained, Normal pressure boils 2-4min, is down to room temperature, in 5000-7000r/min traditional vacuum 8-10min, collects hydrolyzed solution, successively through silicon Diatomaceous earth filtration, ultrafiltration, lyophilizing i.e. obtain bee pupa zymolyte；

Described solion is containing sodium ion 15-20mg/L, zinc ion 10-15mg/L, potassium ion 8-12mg/L, calcium ion 6-10mg/L and the aqueous solution of magnesium ion 5-8mg/L；

Described compound enzyme quality group becomes: flavor protease: acid protease: lipase=1-3:1-2:0.1-0.3；

Described traditional vacuum condition is temperature 5-8 DEG C, vacuum-0.02--0.04MPa.

Functional compound protein compositions the most as claimed in claim 6, it is characterised in that the quality group of described biological demulsifying agent becomes: Glycolipid class: lipopeptid class: cell wall combines class=3-5:1-3:1-2.

Functional compound protein compositions the most as claimed in claim 7, it is characterised in that the quality of described glycolipid class biological demulsifying agent Consist of: rhamnolipid: alkyl polyglucoside=6-8:3-5.

9. the preparation method of functional compound protein compositions as described in claim 1-8 is arbitrary, it is characterised in that comprise the steps:

The preparation method of functional compound protein compositions the most as claimed in claim 9, it is characterised in that described functional compound egg White compositions, mainly prepared by major ingredient and adjuvant by following parts by weight: described major ingredient is made up of the raw material of following parts by weight: Soybean protein isolate 70 parts, concentrated lactoalbumin 40 parts, phosphatidase 20 part；Described adjuvant is Folium Stevlae Rebaudianae 2 parts, food fibre powder 30 parts, Wheat Sprout Powder 30 parts, antifreeze peptide 15 parts, bee pupa zymolyte 10 parts；

Preparation method, comprises the steps: