CN105995984A

CN105995984A - Compound albumen powder capable of improving immunity and preparation method thereof

Info

Publication number: CN105995984A
Application number: CN201610348036.8A
Authority: CN
Inventors: 李秉典
Original assignee: Yiwu City Xin Yu Biotechnology Co Ltd
Current assignee: Yiwu City Xin Yu Biotechnology Co Ltd
Priority date: 2016-05-23
Filing date: 2016-05-23
Publication date: 2016-10-12

Abstract

The invention discloses compound albumen powder capable of improving immunity and a preparation method thereof. According to the preparation method, soybean protein isolate, whey protein concentrate and phospholipid are used as main raw materials; stevia rebaudiana is processed at low temperature to prepare stevia rebaudiana extract so as to furthest maintain the effective ingredient and bioactive ingredient of stevia rebaudiana, and the stevia rebaudiana extract has the effects of high extraction rate, pure flavor, natural color and no licorice flavor, and can be scientifically compounded with main materials; antifreeze polypeptide, dietary fiber powder, bee pupa zymolyte and wheat malt flour are added to prepare the compound albumen powder which has proper taste, good solubility, comprehensive nutrition, strong bioactivity and wide applicable group and is capable of improving immunity. The preparation method adopts a non-thermal processing technology, has high work efficiency, little energy consumption, environmental friendliness and no pollution, and can be used for reducing introduction of food additive and chemical additive, effectively improving food safety, improving food quality and reducing production cost, so that the low-carbon production target is achieved, and large-scale industrialized development is realized.

Description

A kind of compound protein powder improving immunity and preparation method thereof

Technical field

The present invention relates to egg albumen powder, particularly to a kind of compound protein powder improving immunity and preparation method thereof.

Background technology

Protein is the main composition material of human body, is again the main matter of human life activity.Protein adds nucleic acid, is raw The principal mode that life exists.Protein is in the meaning that ancient Latin original meaning is " primarily ", though it is an one's share of expenses for a joint undertaking of nutrient substance, But be in the status at various one center of nutrient substance, its effect mainly: 1, be used for manufacturing muscle, blood, skin and Various organs.2, health is helped to manufacture the tissue that new organization breaks down with replacement.3, to the various nutrition of cell delivery.4, adjust The balance of joint internal water.5, for immune system manufacture to antibacterium and the antibody of infection.6, help wound blood to condense and heal Close.7, manufacturing enzyme in vivo, helping food conversion is energy.Human body lacks protein can be fatal, the egg in human body The 16% of white matter about percentage of liveweight, the people of a 60kg body weight, internal have 10～11kg protein, if human body is lost internal More than the 20% of protein, vital movement will be forced out of service.The shortage symptom of common protein is that metabolic rate declines, raw Life becomes fragile and easily sick, and long-term effect infringement on organ is affirmative, the shortage of protein, the most again with the lacking of energy Weary common existence, common are upgrowth and development of children slow, weight loss.Middle-aged and elderly people be easily generated indifferent, easily enrage, emotion Instability, anemia and skinny and wizened or edema, and the secondary disease because of hypoimmunity easy infection.

Lactalbumin can resist various immunologic challenges, can promote humoral immunization and cellular immunization.The most also find that lactalbumin can be right Anti-microbial infection, such as Salmonella, streptococcal pneumonia etc..Soybean protein accounts for the 40% of soy content, is frumentum food 4～5 times of thing.Soy proteinaceous aminoacid composition is close with milk protein, and in addition to methionine is lower slightly, remaining must ammonia Base acid content is the abundantest, is vegetalitas complete protein, can be equal to animal proteinum in nutritive value.Soybean protein is to strong The positive role of Kang Suoqi mainly has: soybean protein contributes to reducing blood plasma cholesterol level, can stop urine calcium loss, help rush Enter bone health.Additionally the absorption of soybean protein has the effect promoting renal function, and soybean protein can be pacified relatively as nephrotic Full protein source.

Phospholipid is the lipids containing phospholipid root, is Life Base material.And cell membrane is just by about 4 0% protein and 50% The lipid (phospholipid is main) of left and right is constituted.It is by compositions such as lecithin, lipositol, cephalin.These phospholipid are the most right Each position and each organ of human body play corresponding function.All containing phospholipid in human body all cells, it sustains life activity Basic substance.Phospholipid, to activating cell, maintains metabolism, basal metabolism and hormonal equilibrium secretion, strengthens exempting from of human body Epidemic disease power and regeneration power, can play great effect.From the point of view of Gai Kuoing, the basic training of phospholipid is with being: strengthen mental, stable nerve, Balance endocrine, improves immunity and regeneration power, diuresis of detoxifying, blood clean, and vigorous and graceful skin keeps youth, slow down aging.

At present, preparing the more of compound protein powder with soybean protein isolate, lactalbumin, phospholipid for raw material, have is main to supplement For the purpose of human nutrition: Chinese patent CN 104770743 A discloses a kind of compound protein powder and preparation method thereof.Compound protein Powder contain soybean protein powder, lactalbumin powder, highland barley soy Protein powder, potato protein meal, Sweet Potato Protein Powder, collagen protein powder, Semen Pisi sativi protein powder, walnut powder, meringue powder, yolk powder, soybean phospholipid, Pollen Brassicae campestris, sweeting agent, essence.Its preparation method That raw material is toasted, cools down, mixes, packs, finished product.It is former that proportioning raw materials selected by this invention meets modern health preserving Reason, scientific formulation is reasonable, has the effect of good supplementary needed by human body nutrition, and good mouthfeel, unique flavor.China is specially Profit CN 105146043 A discloses a kind of Lepidinm meyenii Walp egg albumen powder, major ingredient and adjuvant are mixed with and form, and described major ingredient is pueraria root powder And soybean protein powder；Described adjuvant, including lecithin, lactalbumin powder, fructose, vanillin, non-dairy creamer, oligomeric fruit Sugar, trace element, compound vitamin, silicon dioxide and Konjac glucomannan；Its dispensing mass ratio is: pueraria root powder 2.2-3.8 part, big Soybean protein powder 55-80 part, lecithin 1.5-2.5 part, lactalbumin powder 4.9-8.5 part, fructose 7.3-9.2 part, perfume (or spice) Blue element 0.0015-0.0035 part, non-dairy creamer 4.8-8.2 part, oligofructose 5.1-6.7 part, trace element 0.9-1.5 part, Compound vitamin 0.08-0.22 part, silicon dioxide 0.07-0.2 part and Konjac glucomannan 0.08-0.23 part, after dispensing is pulverized, Cross 100-150 mesh sieve, mix homogeneously, to obtain final product.This invention effectively supplements the nutrient substance lacked, and regulates nutritive equilibrium comprehensively, Improve immunity, strong health.Chinese patent CN 103005464 B discloses a kind of lactalbumin composition, method and purposes. The invention provides multiple lactalbumin composition, it is suitable as i.e. using fluid composition as nutrient formulation, and its storage is stable And containing high-caliber intact whey protein content.

Have by prevention and treatment disease for the purpose of: Chinese patent CN 103584089 A discloses a kind of many nutritive composite proteins The processing method of powder, is made up of the raw material of following weight parts: Human-like Collagen 15, cattle colostrums 15, soybean protein 350, Lactalbumin 550, Ganoderma 5, Flos Lonicerae 5, Radix Scrophulariae 5, Radix Ophiopogonis 3, Folium Ginkgo 2, Radix phyllostachydis bambusoidis 3, Radix Salviae Miltiorrhizae 3, Rhizoma Fagopyri Dibotryis 2, Herba Lysimachiae Klattianae 3, roselle juice 30；The processing method that the invention provides a kind of many nutritive composite protein powders, its procedure of processing letter Single, raw material sources are wide, safety and Health；Use the protein powder that this inventive method produces, nutritious, instant, absorb, Utilization rate is high, can reduce the intestines and stomach and bear, and can avoid the absorption of excess fat and cholesterol, energy while supplementary protein Efficiently reduce the onset risk of cardiovascular disease.Chinese patent CN 104839736 A discloses one can prevent senile dementia The egg albumen powder of disease, it is made up of the raw material of following weight: soybean protein powder 80-90, lactalbumin powder 20-30, Flos Chrysanthemi Indici lyophilizing Powder 10-12, Semen Juglandis 10-12, Poria 12-15, Semen Sesami Nigrum 6-8, Corm Eleocharitis 12-15, Semen Nelumbinis 10-12, Fructus Jujubae 12-15, Rhizoma Dioscoreae 10-12, Flammulina velutiper (Fr.) Sing 10-12, Hericium erinaceus (Bull. Ex Fr.) Pers. 15-20, Tremella 12-15, Radix Polygalae 10-12, Thallus Laminariae (Thallus Eckloniae) 10-12, He Shou Crow 10-12, Herb Gynostemmae Pentaphylli 12-16, Folium Ginseng 2-15, Folium Ginkgo 12-15, Rhizoma Acori Graminei 10-12, nourishing additive agent 3-5.Should The egg albumen powder of invention, nutritious, it is eaten for a long time and can play nourishing the brain and tranquilizing the mind, the liver and the kidney tonifying, improve the vigor of brain cell, To brain-strengthening, there is effect of senile dementia in prevention.Chinese patent discloses CN 103768585 B deer collagen albumen battalion Support compositions, be made up of deer collagen albumen, glucosamine, hyaluronic acid, soybean isoflavone and tea polyphenols.Described compositions In containing the deer collagen albumen of 70%～95% (percentage by weight), 1%～glucosamine, 1%～10% of 10% (percentage by weight) The hyaluronic acid of (percentage by weight), 0.1%～the soybean isoflavone of 5% (percentage by weight) and 0.1%～6% (weight percent Than) tea polyphenols.Described deer collagen albumen is become with Corium Cervi collagen protein groups by collagen from deer sinew, deer-bone collagenous albumen, Ligamentum cervi The weight ratio of collagen protein, deer-bone collagenous albumen and Corium Cervi collagen protein is 1:1:1.The molecular weight distribution of described deer collagen albumen Below 1500 dalton.This invention, with deer collagen albumen as main component, is aided with glucosamine, hyaluronic acid, Semen sojae atricolor The tea polyphenols of isoflavone and antioxidation define have enhancing bone density, osteoporotic alimentation composition can be significantly improved.

Also have and mainly solve solubility problem: Chinese patent CN 103271166 A disclose a kind of soy-bean whey composite powder and Compound method.This soy-bean whey composite powder is with Non-transgenic soybean egg albumen powder and lactalbumin as primary raw material, adds Semen sojae atricolor ovum Phospholipid, soybean isoflavone, soybean oligo saccharide and other trace element, nutrition is more rich, more comprehensively, more equalizes.Containing multiple sky So active component, for high-protein nutritious food.There is the distinctive fragrance of soybean protein, smooth in taste, pure taste, strong, I.e. rush instant, supplement the nutrients the most comprehensively.In warm water somewhat stirring can rapid solution, there is intrinsic fragrance and abnormal smells from the patient, And it is the most not stratified.Chinese patent CN 102630947 B discloses the production method of a kind of low-molecular-weight nutritional protein powder, The method comprises the following steps: with soybean protein powder and lactalbumin powder as raw material, adds 70～90 DEG C of water and stirs into and mix uniformly Suspension, passes through membrane filtration: first select 5000 daltonian filter membranes under the conditions of being not less than 60 DEG C while hot, and filtrate is again by 500 Daltonian filter membrane；The filtrate that obtain is spray-dried, add spray drying powder body weight 0.5～1.5% lecithin mixing all Even.The protein powder molecular weight that this invented technology prepares is little, aminoacid composition is scientific and reasonable, easily absorbs；Profile is pure white, Attractive in appearance, without bean raw meat, milk fishy smell；Soluble in water, also can comparatively fast dissolve in cold water, it is easy to dispersion, be particularly well-suited to protein Shortage crowd, weak and sickly and tumor patient.

The most single egg albumen powder of patent disclosed above or compound protein powder, either animal albumen powder or vegetable protein powder Main concentration solves the trophism of protein powder, functional (dissolubility, stability, spumescence, emulsibility, gelation etc.) With the prevention aspect such as disease, and the most more or less there is following defect: 1) containing substantial amounts of excess saturated fat, cholesterol, High heat；2) in processing technique, with the addition of substantial amounts of food additive such as pigment, sweeting agent, preservative etc.；3) dissolubility, Poor stability, reconstitutes uneven, conglomeration, lamination easily occurs；4) aminoacid composition is comprehensive not, and each nutritional labeling forms Equilibrium not；5) consumer group relates to middle-aged and elderly people and women is less, has limitation；To the bioactivity research of egg albumen powder relatively Few, the biological activity of protein powder, titer are its trophism, the comprehensive embodiment of many-sided character such as functional, in processing and storage Process of depositing is affected very big, especially in active microorganism microbial inoculum, enzyme preparation by external environment (temperature, humidity, illumination, oxygen) Manifest the most prominent etc. technical field.

For a long time, in terms of protein powder biological activity, the impact that have studied high temperature to proteins biological activity is mainly concentrated, but Ignore the impact of freezing.Antifreeze protein (Antifreeze Protein, AFP), also known as thermal hysteresis protein (Thermal Hysteresis Protein, THP), ice associated proteins (Ice Binding Proteins) or ice crystal histone (Ice Structuring Proteins).Antifreeze protein can be by being combined with specific ice crystal surface, and non-colligative property ground reduces solution Freezing point or suppression ice-crystal growth, have low-temperature protection function.Antifreeze protein can be used for the Excised Embryos of human body or animal organ, Improve its freezing quality；Can improve the freezing tolerance of specified microorganisms, when reducing Frozen food store, the formation of ice crystal and recrystallization are to food Product structural damage, improves food quality.Antifreeze protein can be applied as a kind of senior antifreezing agent, in medical science, cosmetics, life Thing and field of food all have a wide range of applications.

Processing, transport and storage science at protein powder compounds antifreeze protein (or related substances), not only can improve albumen The biological activity of matter powder, trophism, functional, and the moisture of egg albumen powder can be properly increased within the same shelf-life, fall Low production cost.The Patents document or the technical literature that solve the frost resistance of compound protein powder or compositions in prior art are less, Almost without.

Summary of the invention

Solved by the invention technical problem is that the defect overcoming existing compound protein compositions, with soybean protein isolate, condensed whey Albumen and phospholipid are primary raw material, and Folium Stevlae Rebaudianae machining at low temperature is become Stevia rebaudiana (Bertoni) Hemsl extract, compound with its science, and add freeze proof many Peptide, food fibre powder, bee pupa zymolyte, Wheat Sprout Powder, preparation is a kind of, and mouthfeel is suitable, dissolubility good, comprehensive nutrition, life The compound protein powder that thing activity is strong, suitable population can improve immunity widely.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of compound protein powder improving immunity, is mainly prepared by the raw material of following parts by weight: soybean protein isolate 50-90 Part, concentrated lactoalbumin 60-80 part, food fibre powder 45-65 part, Wheat Sprout Powder 45-65 part, phosphatidase 3 5-55 part is freeze proof Polypeptide 20-30 part, bee pupa zymolyte 5-15 part, Folium Stevlae Rebaudianae 1-3 part；

Preferably, the described compound protein powder improving immunity, mainly prepared by the raw material of following parts by weight: soybean separation protein White 60-80 part, concentrated lactoalbumin 65-75 part, food fibre powder 50-60 part, Wheat Sprout Powder 50-60 part, phosphatidase 4 0-50 Part, antifreeze peptide 23-27 part, bee pupa zymolyte 8-12 part, Folium Stevlae Rebaudianae 1.5-2.5 part；

It is highly preferred that the described compound protein powder improving immunity, mainly prepared by the raw material of following parts by weight: Semen sojae atricolor separates Protein 70 part, concentrated lactoalbumin 70 parts, food fibre powder 55 parts, Wheat Sprout Powder 55 parts, phosphatidase 45 parts, antifreeze peptide 25 parts, bee pupa zymolyte 10 parts, Folium Stevlae Rebaudianae 2 parts；

Further, described food fibre powder be by dietary fiber through physics, chemical or biological method process and obtain solvable Property content of cellulose is high, biological activity strong, have important, the cellulose of positive role to human body beneficial flora, with full diet Fiber is compared, and it is functional, biological activity is more powerful；

Preferably, described food fibre powder is by one or more warps in inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber Micronizing, extruding puffing and enzyme enzymolysis and obtain；

It is highly preferred that the preparation method of described food fibre powder, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 4-6:2-4:1-3:1-2 in mass ratio uniformly mixes, micronizing to particle diameter 3-6 μm, adjusts ground product moisture and contains Amount is 17-19%, in screw speed 160-180r/min, temperature 185-200 DEG C condition extruding puffing, adds puff quality 2-4 Water again, room temperature 200-400W, 35-40KHz condition supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, arteries and veins Rush time 400-600 μ s, under the conditions of pulse frequency 200-400Hz, carry out high voltage pulse electric field processing 10-15min；Regulate with lactic acid PH value is 4.5-6.5, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution subtracts Pressure concentrates, lyophilization i.e. obtains food fibre powder；

Described enzyme be cellulase, xylanase, laccase, pectase, tannase in mass ratio 2-4:1-3:1-3:0.5-1.5:0.2-1 uniformly mixes.

Further, described Wheat Sprout Powder is with Semen Tritici aestivi as primary raw material, successively through ultrasonic cleaning, the instantaneous process of microwave, ultrasonic The techniques such as auxiliary biological enzymolysis soaks, germinates, is dried, low-temperature grinding and prepare；

Preferably, the preparation method of described Wheat Sprout Powder, comprise the steps: to be placed on equipped with 0.04-0.06% bicarbonate Semen Tritici aestivi In power 200-400W, frequency 20-30KHz room temperature ultrasonic cleaning 3-5min in the ultrasonic washing unit of sodium solution, take out, drip Dry, in frequency 2450MHz, power 3000W, temperature 38-42 DEG C, thickness of feed layer 2-4cm microwave drying 6-8s, then put temperature Degree for 33-36 DEG C, pH value be 6-8 soak in soak 4-6h, it is 0.3-0.5% that soak contains mass percent concentration Compound enzyme, ventilate once every 15-20min, ventilation pressure 0.13-0.15MPa, simultaneously in electric field intensity 10-15kV/cm, Burst length 100-300 μ s, pulse frequency 60-80Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 40-45%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20-24 DEG C, during dark germination Between be 20-24h, through germinate after wheat drying to moisture be 5-8%, low-temperature grinding, cross 80-100 mesh sieve i.e. obtain Semen Tritici aestivi Bud powder；

Described compound enzyme is that cellulase, 1,4 beta-glucanase, xylanase 2-4:1-3:1-3 in mass ratio uniformly mixes.

Further, described antifreeze peptide is the plant extract prepared for raw material with the natural plants seed containing abundant freeze proof substrate Thing is compounding with sericin peptide taken science to be formed, and is not only remarkably improved the compound protein powder frost resistance that can improve immunity, and then significantly carries Its biological activity high, trophism, functional, and the compound protein that can improve immunity can be properly increased within the same shelf-life The moisture of powder, reduces production cost；Add again dissolubility and the stability of the compound protein powder that can improve immunity simultaneously, Create beyond thought beneficial effect, be particularly suitable as improving the freezeproof protectant of the compound protein powder of immunity；

Preferably, the quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=12-29:3-9；

Preferably, described plant extract be with winter rye, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed in one or more as raw material, Through high-pressure electrostatic, high-pressure pulse electric auxiliary bigcatkin willow acid soak, refrigerating process after the most blended enzyme subsection enzymolysis and prepare；

It is highly preferred that the preparation method of described plant extract, comprise the steps: winter rye, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici Seed 9-17:2-4:1-2 in mass ratio uniformly mixes, sabot, first processes 5-7min in electric field intensity 5-7kV/cm high-pressure electrostatic； Then soaking at room temperature 2-4h in the salicylic acid solution that concentration is 10-30mg/L, simultaneously in electric field intensity 10-20kV/cm, pulse Time 150-250 μ s, carries out high voltage pulse electric field processing under the conditions of pulse frequency 150-250Hz；Rinse, drain, in 3-5 DEG C Stand 18-24h, the most successively at 1-3 DEG C of cold preservation 2-4d ,-3--5 DEG C of freezing 1-3d ,-15--18 DEG C of freezing 10-15h, put immediately In outdoor in intensity of illumination 0.5-5 ten thousand Lx natural lighting 5-7h, compound half is made to pulverize immediately after thawing, ground product grain Footpath 0.5-1.5mm, is subsequently added into the water of ground product quality 4-6 times, is 3.5-5.5 with breast acid for adjusting pH value；It is eventually adding mixing The mixed enzyme of feed liquid quality 1-2%, first in 35-50 DEG C of enzymolysis 10-30min, then in 50-60 DEG C of enzymolysis 20-40min；Enzyme Solve liquid filtration, filtrate reduced in volume to solid content is that 15-25% i.e. obtains plant extract；

Described mixed enzyme is cellulase, protease, amylase, pectase, lipase 8-10:5-7:2-4:1-3:1-2 in mass ratio Uniformly mixing.

Further, described bee pupa zymolyte with the queen bee nit of 2-3 age in days and the drone pupa of 11-12 age in days as raw material, warp Freeze proof protection, freezing crushing, vacuum outgas eliminating sargassum smell, compound enzyme biological enzymolysis, biological demulsifying and centrifugation and obtain；

Preferably, the preparation method of described bee pupa zymolyte, comprise the steps: fresh queen bee nit and drone pupa by matter Measure more uniform mixing than 2-5:1-3, add the sericin peptide taken solution that mass percent is 4-7% and the 0.1-0.3 of mixture quality 1-2 times Solion again, stirs, and in-18--22 DEG C of freezing 12-18min, broken, percentage of damage is 98-100%, broken thing grain Footpath 200-300 μm, is 5-7 with the broken thing pH value of lactic acid regulation, is warming up to 40-50 DEG C, is added thereto to broken material amount The compound enzyme of 0.3-0.5%, mix homogeneously, in vacuum-0.07--0.09MPa degassing eliminating sargassum smell 10-15min, continues enzymolysis 10-15min obtains enzymolysis solution；Enzymolysis solution is put 5000-7000r/min traditional vacuum 8-10min in vacuum centrifuge, from upper and Lower isolated free-fat, emulsion, hydrolyzed solution and residue, be 45-55 DEG C by the emulsion obtained control temperature, add The biological demulsifying agent breakdown of emulsion 20-40min of emulsion quality 0.2-0.4%, in 4000-6000r/min traditional vacuum 2 times after breakdown of emulsion, Interval time, 1-3h, was centrifuged 10-15min every time, merges three centrifugal hydrolyzed solutions obtained, takes the egg of its quality 0.5-0.7% Clearly, put in blender and stir 10-12min in rotating speed 800-1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains Ovum Gallus domesticus album bubble Foam, adds egg white foams and merges in the hydrolyzed solution obtained, and normal pressure boils 2-4min, is down to room temperature, in 5000-7000r/min Traditional vacuum 8-10min, collects hydrolyzed solution, i.e. obtains bee pupa zymolyte through kieselguhr filtration, ultrafiltration, lyophilizing successively；

Further, described solion be containing sodium ion 15-20mg/L, zinc ion 10-15mg/L, potassium ion 8-12mg/L, Calcium ion 6-10mg/L and the aqueous solution of magnesium ion 5-8mg/L；

Further, described compound enzyme quality group becomes: flavor protease: acid protease: lipase=1-3:1-2:0.1-0.3；

Further, described traditional vacuum condition is temperature 5-8 DEG C, vacuum-0.02--0.04MPa；

Further, described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall combine one or more in class biological demulsifying agent Combination；

Preferably, the quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall combines class=3-5:1-3:1-2；

Preferably, described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl polyglucoside；

It is highly preferred that the quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=6-8:3-5.

Present invention also offers the preparation method of the above-mentioned compound protein powder improving immunity, comprise the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first Folium Stevlae Rebaudianae is put into equipped with The ultrasonic washing unit of 0.1-0.3% sodium bicarbonate solution cleans 3-5min in 200-400W, 20-40KHz, drains, put micro- Ripple be dried in power 3-5kW, thickness of feed layer 2-4cm, 80-100 DEG C, be dried 2-4min, be then immersed in mass percent For 10-15min in the sericin peptide taken solution of 4-7%, take out, pulverize immediately after-18--22 DEG C of freezing 15-25min, freezing Thickness of feed layer 3-5cm, ground product particle diameter 0.3-0.5mm, it is subsequently added into the water of ground product quality 8-12 times, with breast acid for adjusting pH Value is 3.5-5.5, at room temperature in electric field intensity 25-35kV/cm, burst length 300-500 μ s, pulse frequency 200-300Hz Under the conditions of carry out high voltage pulse electric field processing 10-15min；Then under the conditions of power 150-300W, microwave exposure is carried out in room temperature Extract 8-12min, simultaneously at power 200-300W, under the conditions of frequency 30-40KHz, carry out ultrasonic assistant extraction；Add and extract The digestive enzyme of liquid quality 0.2-0.6%, in 40-50 DEG C of enzymolysis 30-50min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 2-4%, puts into stirring Machine stirs 10-12min in rotating speed 800-1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, Ovum Gallus domesticus album is steeped Foam adds in enzymolysis solution, and normal pressure boils 1-3min, is rapidly decreased to room temperature, and 80-100 eye mesh screen filters, and filtrate reduced in volume is to solid Shape thing content obtains Stevia rebaudiana (Bertoni) Hemsl extract to 8-12%；

Described digestive enzyme is that cellulase, protease, pectase 1-3:1-2:1-2 in mass ratio uniformly mixes；

2) batch mixing: the 40-60% of antifreeze peptide quality and the 4-6% of food fibre powder quality is sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, Uniformly mixing, at power 300-500W, carries out ultrasonic dissolution 10-15min, obtains mixed liquor under the conditions of frequency 35-45KHz；

3) lyophilizing: mixed liquor puts in dry storehouse quick-freezing to-40～-50 DEG C, and pre-freeze 2～3h at-40～-50 DEG C；Then will After being evacuated to 15～20Pa in dry storehouse, progressively heat up in 8-10h and control mixed liquor final temperature-15～-18 DEG C； Then, in the case of in dry storehouse, vacuum is constant, in regulation dry storehouse, temperature makes it progressively be warming up to room temperature in 5-7h, arrives Lyophilized powder moisture less than 4% time discharging, under aseptic condition successively with soybean protein isolate, concentrated lactoalbumin, Wheat Sprout Powder, Phospholipid, bee pupa zymolyte, residue antifreeze peptide, residue food fibre powder uniformly mix to improve the compound protein of immunity Powder.

Beneficial effect:

Folium Stevlae Rebaudianae, with soybean protein isolate, concentrated lactoalbumin and phospholipid as raw material, is first used ultrasonic cleaning to kill by the present invention Worm is gone out ovum, killing microorganisms, removal pesticide residues and heavy metal ion etc., substantially increases the foodsafety of finished product；Use Microwave drying make Folium Stevlae Rebaudianae in short-term, mainly enzyme denaturing while low-temperature explosion-puffing drying, prevent extract brown stain；Containing silk The aqueous solution of glue peptide soaks rehydration, chilled and the Folium Stevlae Rebaudianae active substance that causes and cold quick property composition can be reduced to greatest extent Loss, improve the extraction ratio of Folium Stevlae Rebaudianae bioactive ingredients, antifreeze peptide, food fibre powder extracted with Folium Stevlae Rebaudianae simultaneously Thing science compounds, and preserves for follow-up lyophilizing technique and finished product and provides good cryoprotective agent, improves cryoprotective effects, prevent Protein freeze denaturalization, improves its biological activity and titer；Omnidistance employing extract at low temperature technique, by ultrasonic cleaning, microwave deactivating enzyme Organically combine with expanded, high-pressure pulse electric extraction, ultrasonic wave added microwave extraction, biological enzymolysis, the extract maximum limit obtained Degree ground remains the natural colored of Folium Stevlae Rebaudianae, local flavor and mouthfeel, makes bioactive substance and nutrition guarantor that extract contains simultaneously Strong content of material maximizes, and raw material extraction ratio and utilization rate are the highest, reduce extraction cost；Use egg white foams prepared by Ovum Gallus domesticus album To enzymolysis solution flocks in short-term, eliminate the Radix Glycyrrhizae taste of stevioside further, improve dissolubility and the stability of finished product；By portion Divide food fibre powder, antifreeze peptide addition Stevia rebaudiana (Bertoni) Hemsl extract to not only increase cryoprotective effects and also can improve the solid content of batch mixing, Reduce moisture, shorten freeze-drying time, improve lyophilizing efficiency, reduce freezing loss, improve stablizing of lyophilized powder Property, through ultrasonic dissolution, further enhancing the homogeneity of batch mixing, stability and dissolubility, so improve finished product homogeneity, Stability and dissolubility；The functional dietary fiber powder using the present invention to prepare replaces existing anticaking agents, significantly improves finished product Disintegration rate, improve finished product dissolubility and dissolve after aesthetic quality, substantially prolongs the shelf-life of finished product simultaneously；? It is the most prepared that a kind of mouthfeel is suitable, dissolubility good, comprehensive nutrition, biological activity strong, suitable population can improve immunity widely Compound protein powder.Concrete test effect is shown in embodiment 7-10, and concrete know-why is as follows:

1. the antifreeze peptide that prepared by the present invention is the plant extract prepared for raw material with the natural plants seed containing abundant freeze proof substrate Thing is compounding with sericin peptide taken scienology to be formed, and without any chemical substance and food additive, natural freeze proof substrate is comprehensive, abundant, Solubility antifreeze protein and antifreeze peptide content are high, substantially increase the foodsafety of the compound protein powder that can improve immunity, Not only it is remarkably improved the compound protein powder frost resistance that can improve immunity, and then is greatly improved its biological activity, trophism, merit Energy property, and the moisture of egg albumen powder can be properly increased within the same shelf-life, reduce production cost；Add again can simultaneously Improve dissolubility and the stability of the compound protein powder of immunity, create beyond thought beneficial effect, be particularly suitable as multiple The freezeproof protectant of hop protein powder.Plant extract therein is by multiple for the natural plants seed feed science containing abundant freeze proof substrate Join, by high-pressure electrostatic process, high-pressure pulse electric auxiliary Induced by Salicylic Acid, low temperature segmentation Stress treatment and the organic knot of natural lighting Close so that the coercing and induce down of active seed boundary's environment outside containing freeze proof substrate itself, freeze proof matrix components has obtained Comprehensively, the abundantest synthesis and accumulation, maximizing dissolution after biological enzymolysis, antifreeze peptide content can be made to increase, raising simultaneously The dissolubility of plant extract and stability, and then eliminate the problem of the finished product dissolubility difference caused because of antifreeze protein, with meals Food fiber powder, Wheat Sprout Powder, bee pupa zymolyte, phospholipid, Stevia rebaudiana (Bertoni) Hemsl extract science compound, better.

2. micronizing, extruding puffing, supersound extraction, high-pressure pulse electric are extracted and raw by the food fibre powder that prepared by the present invention Thing enzymolysis combination of sciences, gained food fibre powder retentiveness, dilatancy, thickening property are higher, the modified meals prepared than single method Food fiber improves 20-40%, and the impact of acid and alkali, alkali, salt, and soluble fiber cellulose content is high, than prepared by single method changing Property dietary fiber improve 10-30%, it is easier to utilized by lactic acid bacteria, improve lactic acid bacteria in the growth of human body intestinal canal and fertility, Increase kind and the quantity of probiotic bacteria flora, reduce human body intestinal canal pH value, improve human body intestinal canal microecological environment；High adsorption capacity, After modified, the specific surface area of cellulose increases, and network is enriched, and absorption affinity strengthens, and chelates, adsorbs cholesterol and bile The organic molecule ability of acids is higher, the suppression human body absorption to them；Ion-exchange capacity strengthens, to metallic element, especially It is that heavy metal element adsorption effect is higher, effectively prevent body weight for humans metal poisoning；Regulation and the resident time of maintenance intestinal microbial population, Strengthen digestion and the absorbability of intestinal, improve immunity of organisms；Effectively facilitate gastrointestinal peristalsis, slow down and eliminate flatulence, abdominal distention Deng untoward reaction；Powerful embedding effect can prevent environment (oxygen, temperature, illumination, water activity etc.) factor to improving The impact of the compound protein powder of immunity, stabilizes the biological activity of the compound protein powder that can improve immunity further, extends The shelf-life of product.

The preparation method of Wheat Sprout Powder the most of the present invention carries out ultrasonic cleaning, the instantaneous process of microwave and high-tension pulse to the Semen Tritici aestivi of immersion process Rush electric field treatment and effectively prevent soak pollution microbes, it is to avoid generation stink penetrates in Wheat Sprout Powder product, meanwhile, can Effectively strengthen corn seed coat cell wall and the relative permeability of cell membrane, improve the water absorption rate of full cereal seed, promote that seed is sprouted in advance Sending out, improve generation speed and adenosine triphosphate (ATP) content of ultra-oxygen anion free radical, stratification early stage wheat seed is multiple The activation of enzyme and release, endosperm dissolve and functional nutrient composition, bioactive ingredients, the synthesis of antioxidant content, promote Enter the respiratory metabolism of seed, accelerate nutrition and functional materials, bioactive ingredients, the enrichment of antioxidant content are entered Journey, shortens enrichment time, improves nutrition and functional materials, bioactive ingredients, the content of antioxidant content, with life Thing enzymolysis organically combines, can degrade further seed coat cellulose and hemicellulose structure, increases seed coat permeability, activates various life Active substances (endogenous enzymes etc.) vigor is the strongest, and enriching quantity is bigger, and soluble fiber cellulose content increases therewith, for Wheat Sprout Powder In Lactobacillus plantarum provide trophic factors, better significantly；By ultrasonic, microwave, high-voltage pulse electric field technology and biology Enzymolysis organically combines, and shortens soak time, improves germination percentage and germination uniformity；Wheat Sprout Powder heat can be kept to greatest extent Quick property content of material, especially antioxidant (glutathion, inositol hexaphosphate, vitamin C, polyphenol etc.), to greatest extent Ground ensures the natural colored of Wheat Sprout Powder, taste and flavor, may also function as bactericidal action simultaneously；Particularly through the instantaneous appropriateness of microwave After killing embryo, in the case of the biological activity not affecting Fructus Tritici aestivi seed, along with the absorption of moisture, wheat embryo be suppressed but not Affecting every enzyme and the growth of bioactive substance and the dissolving of endosperm, blastogenesis length is the shortest, and Repiration is weak, and sprout damage is low, Improve yield and the quality of Wheat Sprout Powder, significantly improve functional in Wheat Sprout Powder product, trophism, bioactive substance Content, the health care that improve Wheat Sprout Powder (improves body immunity, removes oxygen-derived free radicals, blood fat reducing in human body, prolongs Slow aging), nutritive value and foodsafety.The Fructus Tritici aestivi germination percentage of preparation reaches more than 97%, the long 0.2-0.5mm of bud, length Homogeneous, sprout damage is only 1.5-2.1%, reduces 4.8-7.1% than the loss of existing germination technology, and functional materials content is high, its Middle alpha-aminobutyric acid content is 303.8-315.6mg/100g, glutathion 16.1-18.3mg/100g, inositol hexaphosphate (IP₆) 460.8-484.4mg/100g, dietary fiber 3.7-4.2mg/100g.

4. the bee pupa zymolyte that prepared by the present invention with the queen bee nit of 2-3 age in days and the drone pupa of 11-12 age in days as raw material, warp Freeze proof protection, freezing crushing, vacuum outgas eliminating sargassum smell, compound enzyme biological enzymolysis, biological demulsifying the low temperature such as separate and add with traditional vacuum Work technology and obtain, be effectively increased the utilization rate of queen bee nit and drone pupa, reduce the loss of nutrition and health substance, The bioactive ingredients remaining raw material to greatest extent becomes content of material with nutrition.Aqueous solution containing sericin peptide taken soaks, The loss of pupa worm active substance that is chilled and that cause can be reduced to greatest extent, improve the extraction ratio of pupa worm effective ingredient, with Time also provide natural freezeproof protectant for follow-up lyophilizing technique；Vacuum outgas is organically combined with compound enzyme biological enzymolysis, no Reduced by only the dissolved oxygen content of enzymolysis solution, effectively prevent bioactive ingredients oxidation deterioration, and eliminate queen bee in enzymolysis solution The worm fishy smell of larva and drone pupa, improves the mouthfeel of compound protein compositions, adds appetite, solion and queen bee pupa worm, The science of drone pupa and compound enzyme compounds so that compound enzyme vigor plays maximum, and catalytic capability is more thorough, and then makes raw material In protein and fat fully degrade, improve stability and the clarity of protein composition；By biological demulsifying agent breakdown of emulsion with true Empty centrifugal combination, can make the free-fat in enzymolysis solution and hydrolyzed solution be sufficiently separated, obtain nutrition and bioactive substance contains Amount is abundant, the hydrolyzed solution of clear, adds egg white foams and adds and boil enzyme denaturing, queen bee nit and drone pupa zymolyte more Add stable, transparent, further enhancing the dissolubility of the compound protein powder that can improve immunity, foam type, stability；Simultaneously Containing substantial amounts of queen bee nit polypeptide and drone pupa polypeptide, add the most functional for the compound protein powder of immunity can be improved Bioactive substance, further enhancing the functional and trophism of the compound protein powder that can improve immunity.

5. the preparation method of the compound protein powder that the present invention can improve immunity uses non-thermal technology technology, and work efficiency is high, and the energy disappears Consumption is few, environmentally friendly, pollution-free, decreases the introducing of food additive and chemical assistant, is effectively increased foodsafety, Improve product quality and reduce production cost, it is achieved that low-carbon (LC) productive target, can scale, industrialized development.

It should be noted that this eating effect that can improve the compound protein powder of immunity is that each component is mutually collaborative, interacts As a result, the superposition of not simple raw material function, the science of each raw material components is compounding and extracts, and the effect of generation is considerably beyond respectively Single component function and the superposition of effect, have the most advanced and practicality.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is this Method well known to skilled person.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, The spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, real without departing substantially from the present invention On the premise of matter and scope, the various changes or the change that carry out the material component in these embodiments and consumption fall within this Bright protection domain.

Prepared by embodiment 1 raw material

1. the preparation of food fibre powder:

The preparation method of described food fibre powder, comprises the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 5:3:2:1.5 in mass ratio uniformly mixes, micronizing to particle diameter 5 μm, and adjusting ground product moisture is 18%, in screw rod Rotating speed 170r/min, 192 DEG C of condition extruding puffings of temperature, add the water of puff quality 3 times, room temperature 300W, 40KHz bar Part supersound extraction 12min, then in electric field intensity 30kV/cm, burst length 500 μ s, carry out height under the conditions of pulse frequency 300Hz Pressure impulse electric field processes 12min；It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, in 50 DEG C of enzymes Solve 35min；Enzymolysis solution concentrating under reduced pressure, lyophilization i.e. obtain food fibre powder；

Described enzyme is that cellulase, xylanase, laccase, pectase, tannase 3:2:2:1:0.6 in mass ratio is uniform Mixing.

2. the preparation of Wheat Sprout Powder:

The preparation method of described Wheat Sprout Powder, comprises the steps: to be placed on Semen Tritici aestivi the ultrasound wave equipped with 0.05% sodium bicarbonate solution In power 300W, frequency 25KHz room temperature ultrasonic cleaning 4min in cleaning machine, take out, drain, in frequency 2450MHz, power 3000W, temperature 40 DEG C, thickness of feed layer 3cm microwave drying 7s, then put temperature be 35 DEG C, pH value be 7 soak in soak Bubble 5h, soak contains the compound enzyme that mass percent concentration is 0.4%, ventilates once every 18min, ventilation pressure 0.14MPa, Simultaneously in electric field intensity 12kV/cm, burst length 200 μ s, pulse frequency 70Hz condition carries out high voltage pulse electric field processing, until Wheat water content content is 42%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 22 DEG C, The dark germination time is 22h, and wheat drying to the moisture after germinateing is 6%, low-temperature grinding, crosses 90 mesh sieves and i.e. obtains Semen Tritici aestivi Bud powder；

Described compound enzyme is that cellulase, 1,4 beta-glucanase, xylanase 3:2:2 in mass ratio uniformly mixes.

3. the preparation of plant extract:

The preparation method of described plant extract, comprise the steps: by winter rye, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed by quality More uniform mixing than 13:3:1.5, sabot, first process 6min in electric field intensity 6kV/cm high-pressure electrostatic；Then in concentration it is Soaking at room temperature 3h in the salicylic acid solution of 20mg/L, simultaneously in electric field intensity 15kV/cm, burst length 200 μ s, pulse frequency High voltage pulse electric field processing is carried out under the conditions of 200Hz；Rinse, drain, stand 21h in 4 DEG C, the most successively at 2 DEG C of cold preservation 3d, -4 DEG C of freezing 2d ,-16 DEG C of freezing 12h, be immediately placed at outdoor in intensity of illumination 20,000 Lx natural lighting 6h, makes compound half thaw After pulverize immediately, ground product particle diameter 1.0mm, be subsequently added into the water of ground product quality 5 times, with breast acid for adjusting pH value be 4.5； It is eventually adding the mixed enzyme of mixed liquor quality 1.5%, first in 42 DEG C of enzymolysis 20min, then in 55 DEG C of enzymolysis 30min；Enzyme Solve that liquid filters, filtrate reduced in volume to solid content is 20% i.e. to obtain plant extract；

Described mixed enzyme is that cellulase, protease, amylase, pectase, lipase 9:6:3:2:1.5 in mass ratio is uniform Mixing.

4. the preparation of bee pupa zymolyte:

The preparation method of described bee pupa zymolyte, comprises the steps: fresh queen bee nit and drone pupa 3:2 in mass ratio Uniformly mixing, the sericin peptide taken solution that mass percent is 6% of addition mixture quality 1.5 times and the solion of 0.2 times, stir Mixing uniformly, in-20 DEG C of freezing 15min, broken, percentage of damage is 99%, broken thing particle diameter 250 μm, with the broken thing of lactic acid regulation PH value is 6, is warming up to 45 DEG C, is added thereto to the compound enzyme of broken material amount 0.4%, and mix homogeneously, in vacuum-0.08MPa Degassing eliminating sargassum smell 12min, continues enzymolysis 12min and obtains enzymolysis solution；Enzymolysis solution is put in vacuum centrifuge 6000r/min vacuum from Heart 9min, from top to bottom isolated free-fat, emulsion, hydrolyzed solution and residue, by the emulsion obtained control temperature be 50 DEG C, add the biological demulsifying agent breakdown of emulsion 30min of emulsion quality 0.3%, in 5000r/min traditional vacuum 2 times after breakdown of emulsion, Interval time, 2h, was centrifuged 12min every time, merges three centrifugal hydrolyzed solutions obtained, takes the Ovum Gallus domesticus album of its quality 0.6%, put into and stir Mix in machine and stir 11min in rotating speed 900r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, egg white foams is added Merging in the hydrolyzed solution obtained, normal pressure boils 3min, is down to room temperature, in 6000r/min traditional vacuum 9min, collects hydrolyzed solution, Bee pupa zymolyte is i.e. obtained successively through kieselguhr filtration, ultrafiltration, lyophilizing；

Described solion be containing sodium ion 18mg/L, zinc ion 12mg/L, potassium ion 10mg/L, calcium ion 8mg/L and The aqueous solution of magnesium ion 6mg/L；

Described compound enzyme quality group becomes: flavor protease: acid protease: lipase=2:1.5:0.2；

Described traditional vacuum condition is temperature 6 DEG C, vacuum-0.03MPa；

The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall combines class=4:2:1.5；

The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=7:4.

Food fibre powder that example 2 below-6 is used, Wheat Sprout Powder, plant extract, bee pupa zymolyte are embodiment 1 preparation.

Embodiment 2

A kind of compound protein powder improving immunity, is mainly prepared by the raw material of following parts by weight:

Soybean protein isolate 70 parts, concentrated lactoalbumin 70 parts, food fibre powder 55 parts, Wheat Sprout Powder 55 parts, phosphatidase 45 Part, antifreeze peptide 25 parts, bee pupa zymolyte 10 parts, Folium Stevlae Rebaudianae 2 parts；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=21:6；

Preparation method, comprises the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.2% In the ultrasonic washing unit of sodium bicarbonate solution in 300W, 30KHz clean 4min, drain, put in microwave drying in power 4kW, Thickness of feed layer 3cm, 90 DEG C, dry 3min, be then immersed in 12min in the sericin peptide taken solution that mass percent is 6%, take out, Pulverize immediately after-20 DEG C of freezing 20min, freezing thickness of feed layer 4cm, ground product particle diameter 0.4mm, be subsequently added into ground product The water that quality is 10 times, is 4.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 30kV/cm, and burst length 400 μ s, High voltage pulse electric field processing 12min is carried out under the conditions of pulse frequency 250Hz；Then carry out micro-under the conditions of power 200W in room temperature Amplitude, according to extracting 10min, simultaneously at power 250W, carries out ultrasonic assistant extraction under the conditions of frequency 35KHz；Add extracting solution The digestive enzyme of quality 0.4%, in 45 DEG C of enzymolysis 40min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 3%, puts in blender in rotating speed 900r/min stirs 11min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, is added by egg white foams in enzymolysis solution, normal pressure Boiling 2min, be rapidly decreased to room temperature, 90 eye mesh screens filter, and filtrate reduced in volume to solid content to 10% obtains Folium Stevlae Rebaudianae and extracts Thing；

Described digestive enzyme is that cellulase, protease, pectase 2:1.5:1.5 in mass ratio uniformly mixes；

2) batch mixing: the 50% of antifreeze peptide quality and the 5% of food fibre powder quality are sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, all Even mixing, at power 400W, carries out ultrasonic dissolution 12min, obtains mixed liquor under the conditions of frequency 40KHz；

3) lyophilizing: mixed liquor puts quick-freezing extremely-45 DEG C in dry storehouse, and pre-freeze 2.5h at-45 DEG C；Then take out in dry storehouse Vacuum, to after 18Pa, progressively heats up in 9h and controls mixed liquor final temperature at-16 DEG C；Then vacuum in dry storehouse In the case of constant, regulation dry storehouse in temperature make it progressively be warming up to room temperature in 6h, to lyophilized powder moisture less than 4% time Discharging, under aseptic condition successively with soybean protein isolate, concentrated lactoalbumin, Wheat Sprout Powder, phospholipid, bee pupa zymolyte, Residue antifreeze peptide, residue food fibre powder uniformly mix to improve the compound protein powder of immunity.

Embodiment 3

A kind of compound protein powder improving immunity, is mainly prepared by the raw material of following parts by weight: soybean protein isolate 50 parts, Concentrated lactoalbumin 60 parts, food fibre powder 45 parts, Wheat Sprout Powder 45 parts, phosphatidase 35 parts, antifreeze peptide 20 parts, Pupa Apis Worm zymolyte 5 parts, Folium Stevlae Rebaudianae 1 part；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=12:3；

Preparation method, comprises the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.1% In the ultrasonic washing unit of sodium bicarbonate solution in 200W, 20KHz clean 3min, drain, put in microwave drying in power 3kW, Thickness of feed layer 2cm, 80 DEG C, dry 2min, be then immersed in 10min in the sericin peptide taken solution that mass percent is 4%, take out, Pulverize immediately after-18 DEG C of freezing 15min, freezing thickness of feed layer 3cm, ground product particle diameter 0.3mm, be subsequently added into ground product The water that quality is 8 times, is 3.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 25kV/cm, and burst length 300 μ s, arteries and veins High voltage pulse electric field processing 10min is carried out under the conditions of rushing frequency 200Hz；Then under the conditions of power 150W, microwave is carried out in room temperature Irradiation extracts 8min, simultaneously at power 200W, carries out ultrasonic assistant extraction under the conditions of frequency 30KHz；Add extracting solution quality The digestive enzyme of 0.2%, in 40 DEG C of enzymolysis 30min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 2%, puts in blender in rotating speed 800r/min Stirring 10min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, adds egg white foams in enzymolysis solution, and normal pressure boils 1min, Being rapidly decreased to room temperature, 80 screen filtrations, filtrate reduced in volume to solid content to 8% obtains Stevia rebaudiana (Bertoni) Hemsl extract；

Described digestive enzyme is that cellulase, protease, pectase 1:1:1 in mass ratio uniformly mixes；

2) batch mixing: the 40% of antifreeze peptide quality and the 4% of food fibre powder quality are sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, all Even mixing, at power 300W, carries out ultrasonic dissolution 10min, obtains mixed liquor under the conditions of frequency 35KHz；

3) lyophilizing: mixed liquor puts quick-freezing extremely-40 DEG C in dry storehouse, and pre-freeze 2h at-40 DEG C；Then take out in dry storehouse Vacuum, to after 15Pa, progressively heats up in 8h and controls mixed liquor final temperature at-15 DEG C；Then vacuum in dry storehouse In the case of constant, regulation dry storehouse in temperature make it progressively be warming up to room temperature in 5h, to lyophilized powder moisture less than 4% time Discharging, under aseptic condition successively with soybean protein isolate, concentrated lactoalbumin, Wheat Sprout Powder, phospholipid, bee pupa zymolyte, Residue antifreeze peptide, residue food fibre powder uniformly mix to improve the compound protein powder of immunity.

Embodiment 4

A kind of compound protein powder improving immunity, is mainly prepared by the raw material of following parts by weight: soybean protein isolate 90 parts, Concentrated lactoalbumin 80 parts, food fibre powder 65 parts, Wheat Sprout Powder 65 parts, phosphatidase 55 parts, antifreeze peptide 30 parts, Pupa Apis Worm zymolyte 15 parts, Folium Stevlae Rebaudianae 3 parts；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=29:9；

Preparation method, comprises the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.3% In the ultrasonic washing unit of sodium bicarbonate solution in 400W, 40KHz clean 5min, drain, put in microwave drying in power 5kW, Thickness of feed layer 4cm, 100 DEG C, dry 4min, be then immersed in 15min in the sericin peptide taken solution that mass percent is 7%, take out, Pulverize immediately after-22 DEG C of freezing 25min, freezing thickness of feed layer 5cm, ground product particle diameter 0.5mm, be subsequently added into ground product The water that quality is 12 times, is 5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 35kV/cm, and burst length 500 μ s, High voltage pulse electric field processing 15min is carried out under the conditions of pulse frequency 300Hz；Then carry out micro-under the conditions of power 300W in room temperature Amplitude, according to extracting 12min, simultaneously at power 300W, carries out ultrasonic assistant extraction under the conditions of frequency 40KHz；Add extracting solution The digestive enzyme of quality 0.6%, in 50 DEG C of enzyme 50min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 4%, puts in blender in rotating speed 1000 R/min stirs 12min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, is added by egg white foams in enzymolysis solution, and normal pressure boils Boiling 3min, is rapidly decreased to room temperature, and 100 eye mesh screens filter, and filtrate reduced in volume to solid content to 12% obtains Stevia rebaudiana (Bertoni) Hemsl extract；

Described digestive enzyme is that cellulase, protease, pectase 3:2:2 in mass ratio uniformly mixes；

2) batch mixing: the 60% of antifreeze peptide quality and the 6% of food fibre powder quality are sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, all Even mixing, at power 500W, carries out ultrasonic dissolution 15min, obtains mixed liquor under the conditions of frequency 45KHz；

3) lyophilizing: mixed liquor puts quick-freezing extremely-50 DEG C in dry storehouse, and pre-freeze 3h at-50 DEG C；Then true by taking out in dry storehouse Empty to 20Pa, progressively heat up in 10h and control mixed liquor final temperature at-18 DEG C；Then in dry storehouse, vacuum is not In the case of change, in regulation dry storehouse, temperature makes it progressively be warming up to room temperature in 7h, goes out when being less than 4% to lyophilized powder moisture Material, under aseptic condition successively with soybean protein isolate, concentrated lactoalbumin, Wheat Sprout Powder, phospholipid, bee pupa zymolyte, surplus Remaining antifreeze peptide, residue food fibre powder uniformly mix to improve the compound protein powder of immunity.

Embodiment 5

Soybean protein isolate 60 parts, concentrated lactoalbumin 65 parts, food fibre powder 50 parts, Wheat Sprout Powder 50 parts, phosphatidase 40 Part, antifreeze peptide 23 parts, bee pupa zymolyte 8 parts, Folium Stevlae Rebaudianae 1.5 parts；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=12:9；

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.1% In the ultrasonic washing unit of sodium bicarbonate solution in 400W, 20KHz clean 5min, drain, put in microwave drying in power 3kW, Thickness of feed layer 4cm, 80 DEG C, dry 4min, be then immersed in 15min in the sericin peptide taken solution that mass percent is 4%, take out, Pulverize immediately after-22 DEG C of freezing 15min, freezing thickness of feed layer 5cm, ground product particle diameter 0.3mm, be subsequently added into ground product The water that quality is 12 times, is 3.5-5 with breast acid for adjusting pH value, at room temperature in electric field intensity 35kV/cm, and burst length 300 μ s, High voltage pulse electric field processing 10min is carried out under the conditions of pulse frequency 300Hz；Then carry out micro-under the conditions of power 300W in room temperature Amplitude, according to extracting 8min, simultaneously at power 300W, carries out ultrasonic assistant extraction under the conditions of frequency 30KHz；Add extracting solution matter The digestive enzyme of amount 0.6%, in 40 DEG C of enzymolysis 50min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 2%, puts in blender in rotating speed 1000 R/min stirs 10min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, is added by egg white foams in enzymolysis solution, and normal pressure boils Boiling 1min, is rapidly decreased to room temperature, and 100 eye mesh screens filter, and filtrate reduced in volume to solid content to 8% obtains Stevia rebaudiana (Bertoni) Hemsl extract；

Described digestive enzyme is that cellulase, protease, pectase 1:2:1 in mass ratio uniformly mixes；

2) batch mixing: the 40% of antifreeze peptide quality and the 6% of food fibre powder quality are sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, all Even mixing, at power 300W, carries out ultrasonic dissolution 10min, obtains mixed liquor under the conditions of frequency 45KHz；

3) lyophilizing: mixed liquor puts quick-freezing extremely-40 DEG C in dry storehouse, and pre-freeze 2h at-50 DEG C；Then true by taking out in dry storehouse Empty to 20Pa, progressively heat up in 8-10h and control mixed liquor final temperature at-15 DEG C；Then vacuum in dry storehouse Spend constant in the case of, regulation dry storehouse in temperature make it progressively be warming up to room temperature in 7h, to lyophilized powder moisture less than 4% time Discharging, under aseptic condition successively with soybean protein isolate, concentrated lactoalbumin, Wheat Sprout Powder, phospholipid, bee pupa zymolyte, Residue antifreeze peptide, residue food fibre powder uniformly mix to improve the compound protein powder of immunity.

Embodiment 6

Soybean protein isolate 80 parts, concentrated lactoalbumin 75 parts, food fibre powder 60 parts, Wheat Sprout Powder 60 parts, phosphatidase 50 Part, antifreeze peptide 27 parts, bee pupa zymolyte 12 parts, Folium Stevlae Rebaudianae 2.5 parts；

The quality group of described antifreeze peptide becomes: plant extract: sericin peptide taken=29:3；

Preparation method, comprises the steps:

1) preparation of Stevia rebaudiana (Bertoni) Hemsl extract: according to formula, accurately weighs each component raw material, first puts into Folium Stevlae Rebaudianae equipped with 0.3% In the ultrasonic washing unit of sodium bicarbonate solution in 200W, 40KHz clean 3min, drain, put in microwave drying in power 5kW, Thickness of feed layer 2cm, 100 DEG C, dry 2min, be then immersed in 10min in the sericin peptide taken solution that mass percent is 7%, take out, Pulverize immediately after-22 DEG C of freezing 15min, freezing thickness of feed layer 5cm, ground product particle diameter 0.3mm, be subsequently added into ground product The water that quality is 12 times, is 3.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 35kV/cm, and burst length 300 μ s, High voltage pulse electric field processing 10min is carried out under the conditions of pulse frequency 300Hz；Then carry out micro-under the conditions of power 300W in room temperature Amplitude, according to extracting 8min, simultaneously at power 300W, carries out ultrasonic assistant extraction under the conditions of frequency 30KHz；Add extracting solution matter The digestive enzyme of amount 0.6%, in 40 DEG C of enzymolysis 50min, takes the Ovum Gallus domesticus album of enzymolysis solution quality 2%, puts in blender in rotating speed 1000 R/min stirs 10min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, is added by egg white foams in enzymolysis solution, and normal pressure boils Boiling 3min, is rapidly decreased to room temperature, and 80 eye mesh screens filter, and filtrate reduced in volume to solid content to 12% obtains Stevia rebaudiana (Bertoni) Hemsl extract；

Described digestive enzyme is that cellulase, protease, pectase 3:1:2 in mass ratio uniformly mixes；

2) batch mixing: the 60% of antifreeze peptide quality and the 4% of food fibre powder quality are sequentially added in Stevia rebaudiana (Bertoni) Hemsl extract, all Even mixing, at power 500W, carries out ultrasonic dissolution 15min, obtains mixed liquor under the conditions of frequency 35KHz；

3) lyophilizing: mixed liquor puts quick-freezing extremely-50 DEG C in dry storehouse, and pre-freeze 3h at-40 DEG C；Then true by taking out in dry storehouse Empty to 15Pa, progressively heat up in 10h and control mixed liquor final temperature at-15 DEG C；Then in dry storehouse, vacuum is not In the case of change, in regulation dry storehouse, temperature makes it progressively be warming up to room temperature in 7h, goes out when being less than 4% to lyophilized powder moisture Material, under aseptic condition successively with soybean protein isolate, concentrated lactoalbumin, Wheat Sprout Powder, phospholipid, bee pupa zymolyte, surplus Remaining antifreeze peptide, residue food fibre powder uniformly mix to improve the compound protein powder of immunity.

Embodiment 7 present invention can improve the sensory evaluation test of the compound protein powder of immunity

Invite 24 personnel to the compound protein powder of improved immunity prepared by the embodiment of the present invention 2 with commercially available two kinds similar identical The compound protein powder of date of manufacture is judged, and sense organ is given a mark, wherein specialty and each 12 of layman, professional youth, In middle aged, each 4 of old age, men and women half and half, and layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Beat Dividing and include outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), marking personnel are independent Carry out, be independent of each other, judge result with guarantee accurate.Being added up judging result, equal score value takes approximation, retains whole Number, is specifically shown in Table 1:

Table 1: sensory evaluation statistical result

Note: show significant difference (P ＜ 0.05) with a line internal standard difference lowercase alphabet, the different capitalization of mark represents that difference is extremely notable (P ＜ 0.01), Indicate same letter and represent that difference is not notable (P ＞ 0.05).

Result above shows, the compound protein powder of improved immunity prepared by the present invention is any from outward appearance, quality, local flavor and mouthfeel On the one hand will be substantially better than commercially available lyophilized powder, particularly outward appearance, local flavor and mouthfeel fabulous, remain food to greatest extent former The natural colored of material, taste and flavor, also be adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.

It should be understood that the compound protein powder of improved immunity prepared by embodiment of the present invention 3-6 has above-mentioned experiment equally Effect, between each embodiment and little with above-mentioned experiment effect diversity.

Embodiment 8 present invention can improve the biological activity test of the compound protein powder of immunity

The compound protein powder of improved immunity prepared by the embodiment of the present invention 2 and the powdery saccharifying enzyme of commercially available identical date of manufacture Store 12 months under the conditions of-12 DEG C and 40 DEG C, use in " GB8726-2006 food additive saccharidase preparation " Detection method measures egg albumen powder and the enzyme activity of powdery saccharifying enzyme, calculates enzyme loss rate alive, and enzyme loss rate alive refers to actually detected Enzyme activity and product mark the difference of enzyme activity and account for the percentage rate marking enzyme activity, result such as table 2

Table 2 stores phase enzyme activity loss rate

Result above shows, stores 12 months under the conditions of-12 DEG C and 40 DEG C, and the present invention is functional improves the compound of immunity Egg albumen powder is lived than commercially available powdery glucoamylase enzyme in losing and is reduced by 34% and 10.2% respectively, has excellent temperature storage, and enzyme activity is non- Often stable, the most also the explanation present invention can improve the loss rate pole of other bioactive substance that the compound protein powder of immunity contains Low, there is the most freeze proof, heat resistanceheat resistant performance, long shelf-life.

Embodiment 9 present invention can improve the compound protein powder dissolubility test of immunity

The powder that egg albumen powder is prepared as a kind of raw-food material, with reference to the stability criterion of solid beverage, by dissolution time, dissolving Rear homogeneity is defined as the quality stability index of egg albumen powder, and it the most directly reflects the dissolution degree of product, also reflects product Product inherent quality and stability, be the most important quality control index of egg albumen powder.

Dissolution time: 1:5 in mass ratio adds 55-65 DEG C of warm water, is sufficiently stirred for, the time being completely dissolved；

Homogeneity characterizing method: the most homogeneous, nothing layering 90-100 divides；Sheaf of differentials 80-89 divides；Layering 70-79 divides；Relatively divide Layer 0-69 divides；Severe delamination 50-59 divides；Precipitate less than 49 points (granule is the biggest, quantity is the most for precipitation, and mark is the lowest).

The compound protein powder of improved immunity prepared by the embodiment of the present invention 2 and commercially available same type, same specification, with producing day After the compound protein powder of phase dissolves in glass, standing, observe homogeneity, every 5min observed and recorded result once, be shown in by result Table 3:

Table 3: the compound protein powder clarity testing result of immunity can be improved after dissolving

Result above shows: the present invention can improve the compound protein powder of immunity and compare with commercially available compound protein powder, and dissolubility is preferable, Dissolution time is short, and the stability of solution after dissolving is strong, and homogeneity is strong, and presentation quality is preferable, along with the prolongation of time of repose, city Sell compound protein powder solution liquid phase relatively unstable, produce precipitation, leverage presentation quality and the inherent quality of beverage, with Time also to illustrate that the present invention can improve the compound protein powder inherent quality of immunity preferable, eating effect is safe and stable.

Embodiment 10 present invention can improve the compound protein powder impact on immunity of organisms of immunity

1 experiment purpose

Testing (mouse forced swimming) by exercise tolerance, the checking present invention can improve the raising immunity of the compound protein powder of immunity Power, antifatigue effect.

2 experiment materials and reagent

2.1 for reagent thing:

Commercially available compound protein powder (G1)；Commercially available compound protein powder (G2)；Improved immunity prepared by embodiment of the present invention 2-6 Compound protein powder (G3-G7).

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), the limited public affairs of Nanjing chemical reagent Department；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. laboratory animal

ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, little during experiment The free diet of Mus.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof is public Department；Water-bath: DK-S26 type, upper Nereid grand experimental facilities company limited；Electronic scale: BS224S type, Sartorius Company；Stopwatch, thermometer

5. experiment packet

5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, and often group 10, the 1st group to the 7th component Not Gei the medicine of G1～G7, the 8th group is blank group, gives isopyknic distilled water, the often every average daily gavage of group 1 time, Gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations the 1st group are to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank Group: distilled water 150ml.

6. experimental technique

6.1 swimmings with a load attached to the body experiment lasts are administered after 30min, put mice in swimming trunk, and the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, record mice swimming starts to the dead time, when swimming as mice Between.

After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, Pluck after rest 60min eyeball blood sampling 0.5mL (being not added with anticoagulant), put 4 DEG C of refrigerator 3h, after hemopexis 2000r/min from Heart 15min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.

After the mensuration last of 6.3 hepatic glycogen is administered 30min, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical vertebra Mice is put to death in dislocation, cleans with normal saline, and with after filter paper suck dry moisture, accurately weighs liver 100mg, and hepatic glycogen detects Test kit detection Mouse Liver glycogen content.

The mensuration last of 6.4 blood lactase acid is taken a blood sample after being administered 30min, does not the most bear a heavy burden at the water went swimming 10min that temperature is 30 DEG C Rear stopping.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add after rest 20min after swimming, after swimming Entering in 40 μ L rupture of membranes liquid, the most fully vibration smudge cells lactic acid instrument measures.(blood lactase acid area under curve=5 × (swimming The blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min after front blood lactase acid value+3 × swimming)

7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value

8. statistical method experimental data is usedRepresent, use t inspection to compare between organizing

9. experimental result

9.1 present invention can improve the compound protein powder impact on Mouse Weight of immunity

Each group mice after giving G1～G9 medicine, front, in, shown in post-weight see table respectively, at the beginning of each group mice Initial body weight and weightening finish body weight compare equal no difference of science of statistics (P ＞ 0.05) with matched group, show that G1～G9 medicine is all without significantly Toxicity.Experimental result refers to table 4.

The original body mass of table 4 swimming with a load attached to the body experiment mice, body weight in mid-term and end body weight

9.2 present invention can improve the impact on the mice burden swimming time of the compound protein powder of immunity

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, can be obviously prolonged mice and bear Weight swimming time, has significant difference (P ＜ 0.05), and the present invention can improve compound protein powder G3～the G7 medicine of immunity Compare with blank group, can significantly extend the mice burden swimming time, there is pole significant difference (P ＜ 0.01), and bright Show and be better than G1～G2 medicine.The results detailed in Table 5.

Table 5 can improve the impact on the mice burden swimming time of the compound protein powder of immunity

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

9.3 present invention can improve the compound protein powder of immunity to the impact of blood lactase acid before and after mouse movement

After the compound protein powder of the improved immunity that per os gives the mice present invention, the present invention can improve the compound protein of immunity Blood lactase acid area under curve after mouse movement is compared by powder G3～G7 medicine with matched group significant difference (P ＜ 0.05), Decrease though G1～G2 medicine group Mouse Blood lactic acid area under curve compares with matched group, but and no difference of science of statistics (P ＞ 0.05).The results are shown in Table 6.

Table 6 present invention can improve the compound protein powder of immunity to the impact of blood lactase acid level before and after mouse movement

" * " p < 0.05vs blank；

9.4 present invention can improve the impact on Mouse Liver glycogen of the compound protein powder of immunity

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, and Mouse Liver glycogen content all has bright Aobvious rising, has significant difference (P ＜ 0.05), and the present invention can improve compound protein powder G3～the G7 medicine of immunity Comparing with blank group, Mouse Liver glycogen content all has significantly rising, has pole significant difference (P ＜ 0.01), And it is substantially better than G1～G2 medicine.The results detailed in Table 7.

Table 7 present invention can improve the impact on Mouse Liver glycogen content of the compound protein powder of immunity

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

9.5 present invention can improve the impact on mice serum carbamide of the compound protein powder of immunity

After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, serum urea after mouse movement Content all has significantly reduction, has significant difference (P ＜ 0.05), the present invention can improve immunity compound protein powder G3～ G7 medicine compares with blank group, and after mouse movement, serum urea content all has significantly reduction, has pole significant difference (P ＜ 0.01), and it is substantially better than G1～G2 medicine.The results detailed in Table 8.

Table 8 present invention can improve the impact on mice serum urea content of the compound protein powder of immunity

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

10. experiment conclusion

This experiment is mainly tested by mice burden swimming, and the deposit of detection Mouse Liver glycogen is observed the present invention and can be improved immunity simultaneously The compound protein powder of power improves immunity, the effect of resisting fatigue.Preliminary Results shows as follows:

1, G3～G7 of the present invention can improve the compound protein powder of immunity and all can extend the mice burden swimming time (P ＜ 0.01), And effect is substantially better than the compound protein powder of other G1～G2.

2, biochemistry detection aspect shows, G3～G7 of the present invention can improve compound protein powder each dosage group of immunity all can reduce fortune After dynamic, lactic acid content produced by glucose anerobic glycolysis in mice serum, compares with matched group and has significant difference (P ＜ 0.05), although and the compound protein powder of other G1～G2 also can reduce motion after in mice serum glucose anerobic glycolysis produced Lactic acid content, but compare with matched group, no difference of science of statistics (P ＞ 0.05)；

3, G3～G7 of the present invention can improve compound protein powder each dosage group of immunity and all can significantly improve glycogen in mouse liver Lay in (P ＜ 0.01), and effect is substantially better than the compound protein powder of other G1～G2；

4, metabolic arthritis model finds, G3～G7 of the present invention can improve after the compound protein powder of immunity can significantly reduce mice swimming The content (P ＜ 0.01) of urea in serum, and effect is substantially better than other G1～G2 compound protein powder；

11. conclusions

The above-mentioned experiment proof present invention can improve the compound protein powder of immunity can significantly improve immunity of organisms, improves the muscle power of mice And endurance, reduce urea in serum and the content of lactic acid after mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, Contribute to alleviating the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.

Claims

1. can improve a compound protein powder for immunity, mainly be prepared by the raw material of following parts by weight: soybean protein isolate 50-90 Part, concentrated lactoalbumin 60-80 part, food fibre powder 45-65 part, Wheat Sprout Powder 45-65 part, phosphatidase 3 5-55 part is freeze proof Polypeptide 20-30 part, bee pupa zymolyte 5-15 part, Folium Stevlae Rebaudianae 1-3 part.

The compound protein powder of immunity can be improved the most as claimed in claim 1, it is characterised in that main former by following parts by weight Material preparation: soybean protein isolate 60-80 part, concentrated lactoalbumin 65-75 part, food fibre powder 50-60 part, Wheat Sprout Powder 50-60 part, phosphatidase 4 0-50 part, antifreeze peptide 23-27 part, bee pupa zymolyte 8-12 part, Folium Stevlae Rebaudianae 1.5-2.5 part.

The compound protein powder of immunity can be improved the most as claimed in claim 1, it is characterised in that main former by following parts by weight Material preparation: soybean protein isolate 70 parts, concentrated lactoalbumin 70 parts, food fibre powder 55 parts, Wheat Sprout Powder 55 parts, phosphorus 45 parts of fat, antifreeze peptide 25 parts, bee pupa zymolyte 10 parts, Folium Stevlae Rebaudianae 2 parts.

4. the compound protein powder of the improved immunity as described in claim 1-3 is arbitrary, it is characterised in that the system of described food fibre powder Preparation Method, comprises the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 4-6:2-4:1-3:1-2 in mass ratio Uniformly mixing, micronizing to particle diameter 3-6 μm, adjusting ground product moisture is 17-19%, in screw speed 160-180r/min, temperature 185-200 DEG C condition extruding puffing, the water of addition puff quality 2-4 times, room temperature 200-400W, 35-40KHz condition supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, burst length 400-600 μ s, pulse frequency High voltage pulse electric field processing 10-15min is carried out under the conditions of rate 200-400Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds mixed The enzyme of compound quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution concentrating under reduced pressure, lyophilization i.e. obtain meals Food fiber powder；

5. the compound protein powder of the improved immunity as described in claim 1-3 is arbitrary, it is characterised in that the preparation of described Wheat Sprout Powder Method, comprises the steps: to be placed on by Semen Tritici aestivi in the ultrasonic washing unit equipped with 0.04-0.06% sodium bicarbonate solution in power 200-400W, frequency 20-30KHz room temperature ultrasonic cleaning 3-5min, take out, drain, in frequency 2450MHz, power 3000W, Temperature 38-42 DEG C, thickness of feed layer 2-4cm microwave drying 6-8s, then put temperature be 33-36 DEG C, pH value be the soak of 6-8 Middle immersion 4-6h, soak contains the compound enzyme that mass percent concentration is 0.3-0.5%, ventilates once every 15-20min, Ventilation pressure 0.13-0.15MPa, simultaneously in electric field intensity 10-15kV/cm, burst length 100-300 μ s, pulse frequency 60-80Hz Condition carries out high voltage pulse electric field processing, until wheat water content content is 40-45%；Semen Tritici aestivi after immersion drains, in growing floor Carrying out dark germination, dark germination temperature keeps 20-24 DEG C, and the dark germination time is 20-24h, and the wheat drying after germinateing is to moisture Content is 5-8%, low-temperature grinding, crosses 80-100 mesh sieve and i.e. obtains Wheat Sprout Powder；

6. the compound protein powder of the improved immunity as described in claim 1-3 is arbitrary, it is characterised in that the matter of described antifreeze peptide Amount consists of: plant extract: sericin peptide taken=12-29:3-9；

The preparation method of described plant extract, comprise the steps: by winter rye, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed by quality More uniform mixing than 9-17:2-4:1-2, sabot, first process 5-7min in electric field intensity 5-7kV/cm high-pressure electrostatic；Then exist Concentration be 10-30mg/L salicylic acid solution in soaking at room temperature 2-4h, simultaneously in electric field intensity 10-20kV/cm, burst length 150-250 μ s, carries out high voltage pulse electric field processing under the conditions of pulse frequency 150-250Hz；Rinse, drain, in 3-5 DEG C of standing 18-24h, the most successively at 1-3 DEG C of cold preservation 2-4d ,-3--5 DEG C of freezing 1-3d ,-15--18 DEG C of freezing 10-15h, it is immediately placed at room Outer in intensity of illumination 0.5-5 ten thousand Lx natural lighting 5-7h, make compound half pulverize immediately after thawing, ground product particle diameter 0.5-1.5mm, is subsequently added into the water of ground product quality 4-6 times, is 3.5-5.5 with breast acid for adjusting pH value；It is eventually adding compound The mixed enzyme of liquid quality 1-2%, first in 35-50 DEG C of enzymolysis 10-30min, then in 50-60 DEG C of enzymolysis 20-40min；Enzymolysis Liquid filters, filtrate reduced in volume to solid content is that 15-25% i.e. obtains plant extract；

7. the compound protein powder of the improved immunity as described in claim 1-3 is arbitrary, it is characterised in that described bee pupa zymolyte Preparation method, comprises the steps: uniformly to mix fresh queen bee nit with drone pupa 2-5:1-3 in mass ratio, adds mixing The mass percent of material amount 1-2 times is the sericin peptide taken solution of 4-7% and the solion of 0.1-0.3 times, stirs, in -18--22 DEG C of freezing 12-18min, broken, percentage of damage is 98-100%, broken thing particle diameter 200-300 μm, regulates with lactic acid Broken thing pH value is 5-7, is warming up to 40-50 DEG C, is added thereto to the compound enzyme of broken material amount 0.3-0.5%, mix homogeneously, In vacuum-0.07--0.09MPa degassing eliminating sargassum smell 10-15min, continue enzymolysis 10-15min and obtain enzymolysis solution；Enzymolysis solution is put 5000-7000r/min traditional vacuum 8-10min in vacuum centrifuge, from top to bottom isolated free-fat, emulsion, water Solve liquid and residue, the emulsion obtained control temperature is 45-55 DEG C, adds the biological demulsifying agent of emulsion quality 0.2-0.4% Breakdown of emulsion 20-40min, in 4000-6000r/min traditional vacuum 2 times after breakdown of emulsion, interval time, 1-3h, was centrifuged 10-15min every time, Merge three centrifugal hydrolyzed solutions obtained, take the Ovum Gallus domesticus album of its quality 0.5-0.7%, put in blender in rotating speed 800-1000r/min Stirring 10-12min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, adds egg white foams and merges in the hydrolyzed solution obtained, Normal pressure boils 2-4min, is down to room temperature, in 5000-7000r/min traditional vacuum 8-10min, collects hydrolyzed solution, successively through silicon Diatomaceous earth filtration, ultrafiltration, lyophilizing i.e. obtain bee pupa zymolyte；

Described solion is containing sodium ion 15-20mg/L, zinc ion 10-15mg/L, potassium ion 8-12mg/L, calcium ion 6-10mg/L and the aqueous solution of magnesium ion 5-8mg/L；

Described compound enzyme quality group becomes: flavor protease: acid protease: lipase=1-3:1-2:0.1-0.3；

Described traditional vacuum condition is temperature 5-8 DEG C, vacuum-0.02--0.04MPa.

The compound protein powder of immunity can be improved the most as claimed in claim 7, it is characterised in that the quality group of described biological demulsifying agent Become: glycolipid class: lipopeptid class: cell wall combines class=3-5:1-3:1-2；The quality group of described glycolipid class biological demulsifying agent becomes: Mus Lee's glycolipid: alkyl polyglucoside=6-8:3-5.

9. can improve the preparation method of the compound protein powder of immunity as described in claim 1-8 is arbitrary, it is characterised in that include walking as follows Rapid:

The preparation method of the compound protein powder of immunity can be improved the most as claimed in claim 9, it is characterised in that described improve immunity The compound protein powder of power, is mainly prepared by the raw material of following parts by weight: soybean protein isolate 70 parts, concentrated lactoalbumin 70 Part, food fibre powder 55 parts, Wheat Sprout Powder 55 parts, phosphatidase 45 parts, antifreeze peptide 25 parts, bee pupa zymolyte 10 parts, Folium Stevlae Rebaudianae 2 parts；

Preparation method, comprises the steps: