CN108753891A - A kind of extracting method of crocodile skin peptide - Google Patents
A kind of extracting method of crocodile skin peptide Download PDFInfo
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- CN108753891A CN108753891A CN201810641507.3A CN201810641507A CN108753891A CN 108753891 A CN108753891 A CN 108753891A CN 201810641507 A CN201810641507 A CN 201810641507A CN 108753891 A CN108753891 A CN 108753891A
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Abstract
The invention discloses a kind of extracting method of crocodile skin peptide, step is：The pretreatment of S1, crocodile skin；S2, boiling form a soup；S3, row's soup, and it is pumped into a soup storage device；S4, fermentation hydrolysis form the hydrolyzate after enzyme deactivation；S5, filtering；Clear liquid after the circulating filtration is concentrated into setting concentration concentration, forms the concentrate after clarification by S6, concentration；Concentrate after the clarification is spray-dried according to setting inlet air temperature and setting leaving air temp, forms crocodile skin peptide finished product by S7, spray drying.The extracting method of the present invention, the crocodile skin for propagating crocodile artificially is carried out to the conversion and extraction of small-molecular peptides, its molecular weight of crocodile skin peptide is below 10000 dalton, not only it had remained the original effective ingredient of crocodile but also there is the high property digested and assimilated and utilization rate, it can directly be immediately used by the body without gastro-intestinal digestion.
Description
Technical field
The present invention relates to crocodile deep processings to make extractive technique field, relates in particular to a kind of extraction side of crocodile skin peptide
Method.
Background technology
Crocodile is most ancient reptile, has been survived on earth more than 200,000,000 3 thousand ten thousand years, thus has " living fossil "
Title.《Chinese book on Chinese herbal medicine》The load of volume 26：The first piece of Chinese alligator (Alligatorsinensis), medicine name Chinese alligator first, have stagnation resolvation,
Disperse accumulation, insecticidal function cure mainly lump in the abdomen and gather, under metrorrhagia band, sore scabies, malignant sore, and intestines wind haemorrhoids.Crocodile meat has strengthening the muscles and bones, replenishing essence benefit
The functions such as marrow, it is effective in cure to treating rheumatic ostealgia.Each portion of the whole bodies such as crocodile skin, meat, blood, oil, internal organ is rich in protein, DHA, lecithin
10 kinds of trace elements such as fat, unsaturated fatty acid, iron zinc phosphorus magnesium, 18 kinds with upper amino acid and VitAVitE etc., be one
Kind scientific research value, the higher animal of economic value.Crocodile Gu claims Chinese alligator dragon, earth ridge,《Compendium of Materia Medica》Once its first acrid flavour, property were recorded
Temperature has the effect of blood-breaking is by silt, detumescence and promoting granulation.
It is few using crocodile as the product of raw material on the market at present, it is mostly to cook.And directly edible crocodile skin is substantially simultaneously
The macromolecular structure of the original protein of crocodile skin can not be changed, such product is difficult by people as a kind of macromolecular compound
Body is absorbed and is utilized.
Invention content
It is an object of the invention to be directed to the shortcomings of the prior art, a kind of extraction side of crocodile skin peptide is provided
The crocodile skin for propagating crocodile artificially is carried out the conversion and extraction of small-molecular peptides by method, its molecular weight of crocodile skin peptide is 10000
Hereinafter, not only remained the original effective ingredient of crocodile again with the high property digested and assimilated and utilization rate, it can be without for you
Gastro-intestinal digestion is directly immediately used by the body.
To achieve the goals above, the technical solution adopted in the present invention is：
A kind of the step of extracting method of crocodile skin peptide, the extracting method is：
The pretreatment of S1, crocodile skin, the fresh crocodile skin chopping after slaughtering, put into processing kettle, fresh crocodile are added
The vulcanization oxygenation pretreatment of skin tare weight 6~8%, makes scale loosen, and in favor of the dissolving that falls off of squama, and crocodile skin is made slowly to expand；
S2, boiling；The crocodile skin of water, expansion is put into steaming plant, heating while is put into digestive juice, and synchronous stirring
Into its gelatinization is exercised, stop heating after being warming up to setting heat-preserving range, and kept the temperature according to setting heat preservation duration, heat preservation terminates
Stop stirring afterwards, then standing duration according to setting is stood, and a soup is formed；
S3, row's soup, extract a soup, and be pumped into a soup storage device using draw-out device；
A soup in soup storage device is pumped into hydrolysis device, is warming up to setting and goes out by S4, fermentation hydrolysis
Bacterium temperature is simultaneously kept according to setting sterilizing duration, and trypsase is added and ferments, alkali protease is then added and carries out
This ferment is sent out for the second time, is cooled to setting hydrolysis temperature, and configuration liquid is added by a soup pH value adjustment to neutrality, pepsin is added
Stirring hydrolysis, and keep to enzymolysis duration is set, setting enzyme-removal temperature is warming up to after enzymolysis, and keep to when setting enzyme deactivation
It is long, form the hydrolyzate after enzyme deactivation；
S5, filtering will stand after the hydrolyzate input diatomite and/or activated carbon after the enzyme deactivation, and utilize filtering dress
Filtering is set to clear liquid storage device, then follows the clear liquid according to setting circulating filtration duration using circulation filter
Ring filters；
Clear liquid after the circulating filtration is concentrated into setting concentration concentration, forms the concentrate after clarification by S6, concentration；
S7, spray drying spray the concentrate after the clarification according to setting inlet air temperature and setting leaving air temp
Mist is dried, and crocodile skin peptide finished product is formed.
As the improvement to above-mentioned technical proposal, in step s 2, the boiling sets holding temperature as 50~75 DEG C, if
Surely heat preservation duration ranging from 1~1.5 hour, setting stands duration ranging from 1~1.5 hour.
As the improvement to above-mentioned technical proposal, in step s 4, ranging from 90~100 DEG C of enzyme-removal temperature, setting are set
Enzyme deactivation duration ranging from 10~30 minutes.
As the improvement to above-mentioned technical proposal, in step s 6, the concentration sets concentration concentration range as 10~20
Baume.
As the improvement to above-mentioned technical proposal, in the step s 7, the spray drying sets inlet air temperature ranging from 190
~210 DEG C, set ranging from 90~100 DEG C of leaving air temp.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention solves the problems, such as how high molecular weight protein is effectively absorbed and be utilized by human body, is carried out to albumen small
The conversion and extraction of molecule.The crocodile skin peptide of the present invention is that animal protein pure-natural biological enzymolysis and extraction forms, and is free of anyization
It studies point and hormone, there is no any toxic side effect to human body, be very pure nutriment.It can be dissolved in rapidly without stirring
Water, aqueous solution is limpid transparent, and for white or light yellow transparent liquid, any impurity and sediment will not be precipitated.
Crocodile skin peptide molecular weight is in 10000 dalton hereinafter, have the high property digested and assimilated and utilization rate.It can be with
Without gastro-intestinal digestion, directly it is absorbed by the body.It can effectively ensure supplement of the human body to protein.Reasonable supplement small molecule
Peptide can make one the abundance for keeping nutrition in vivo, and each histoorgan of supply body runs well, and maintains form, and body is made to keep strong
Health and vigor.Rich amino acids and not fatty in small-molecular peptides, are not required to digest direct absorption；Bioactivity by force can be complete
It absorbs；Uncompetitive active absorption；It consumes energy and low does not increase stomach burden.Supplement small-molecular peptides can play apparent adjusting physiology work(
Can, there is vital effect to physiological activities such as the growth of human body, development, metabolism, procreations.
The extracting method of the crocodile skin peptide of the present invention is simple for process, stablizes, of low cost, high income, crocodile skin obtained
Peptide contains the hemoglobin of the efficient antibody and peculiar construction that are extracted out of crocodile body, can improve the immunity of the human body as quick as thought and blood
Liquid oxygen uptake capacity can greatly improve the health quality of human body；The present invention is made crocodile skin peptide and contains strong cancer-resisting substance
It is this containing the substance that can optimize nucleic acid containing strong antibacterial material with the growth factor for preventing tumor neonatal cell
Substance keeps nucleic acid dynamic, and disease resistance is reinforced, while swashing the activity of intravital catalase and SOD, effectively removes internal
Rubbish, containing a large amount of calcium activated and phosphorus, magnesium etc., can be used for treating senile osteoporosis, promote youngster to retarding senility of life
The special efficacies such as virgin growth and development.
The present invention is compared with prior art, and technological progress is significant.1, by crocodile skin carry out biological enzymolysis extraction and
At small-molecular peptides substance, the essential ingredient activity in crocodile skin peptide is superpower, is easily absorbed by the body, and fully adjustable human body is exempted from
Epidemic disease power, has the function of antibacterial anti-inflammatory, can resist the infection of venereal diseases and aids poison；2, product made from the method for the present invention is free of
Any, chemical composition and hormone do not have any toxic side effect to human body, are very pure nutriments；3, using the present invention
Method made from crocodile skin peptide without stirring can be dissolved in water rapidly, aqueous solution is limpid transparent, be light yellow transparent liquid, no
Any impurity and sediment can be precipitated.There is vital effect to physiological activities such as the growth of human body, development, metabolism, procreations,
Promote cellular blood cycle, promote the secretion of male sex hormone, improves sexual function；4, according to theory of traditional Chinese medical science " with dirty, the same gas phase of dirty benefit
Ask " principle, the crocodile skin peptide to human body corresponding organs have good benefiting action, so that people is not eaten directly special
The nutritional ingredient of food is supplemented.
Specific implementation mode
Illustrate that embodiments of the present invention, those skilled in the art can be by this explanations by particular specific embodiment below
Content disclosed by book understands other advantages and effect of the present invention easily.
Embodiment 1：The extracting method of the crocodile skin peptide of the present embodiment is：
The pretreatment of S1, crocodile skin, the fresh crocodile skin chopping after slaughtering, put into processing kettle, fresh crocodile are added
The vulcanization oxygenation pretreatment of skin tare weight 6%, makes scale loosen, and in favor of the dissolving that falls off of squama, and crocodile skin is made slowly to expand；
The crocodile skin of water, expansion is put into steaming plant by S2, boiling, and heating while is put into digestive juice, and synchronous stirring
Into its gelatinization is exercised, stop heating after being warming up to setting heat-preserving range, and kept the temperature according to setting heat preservation duration, heat preservation terminates
Stop stirring afterwards, then standing duration according to setting is stood, and a soup is formed；The boiling sets holding temperature as 50
DEG C, setting heat preservation duration ranging from 1 hour, setting stands duration ranging from 1 hour；
S3, row's soup, extract a soup, and be pumped into a soup storage device using draw-out device；
A soup in soup storage device is pumped into hydrolysis device, is warming up to setting and goes out by S4, fermentation hydrolysis
Bacterium temperature is simultaneously kept according to setting sterilizing duration, and trypsase is added and ferments, alkali protease is then added and carries out
This ferment is sent out for the second time, is cooled to setting hydrolysis temperature, and configuration liquid is added by a soup pH value adjustment to neutrality, pepsin is added
Stirring hydrolysis, and keep to enzymolysis duration is set, setting enzyme-removal temperature is warming up to after enzymolysis, and keep to when setting enzyme deactivation
It is long, form the hydrolyzate after enzyme deactivation；Set ranging from 90 DEG C of enzyme-removal temperature, setting enzyme deactivation duration ranging from 10 minutes；
S5, filtering will stand after the hydrolyzate input diatomite and/or activated carbon after the enzyme deactivation, and utilize filtering dress
Filtering is set to clear liquid storage device, then follows the clear liquid according to setting circulating filtration duration using circulation filter
Ring filters；
Clear liquid after the circulating filtration is concentrated into setting concentration concentration, forms the concentrate after clarification by S6, concentration；
The concentration sets concentration concentration range as 10 Baumes；
S7, spray drying spray the concentrate after the clarification according to setting inlet air temperature and setting leaving air temp
Mist is dried, and crocodile skin peptide finished product is formed.The spray drying sets ranging from 190 DEG C of inlet air temperature, sets leaving air temp range
It is 90 DEG C.
Embodiment 2：The extracting method of the crocodile skin peptide of the present embodiment is：
The pretreatment of S1, crocodile skin, the fresh crocodile skin chopping after slaughtering, put into processing kettle, fresh crocodile are added
The vulcanization oxygenation pretreatment of skin tare weight 8%, makes scale loosen, and in favor of the dissolving that falls off of squama, and crocodile skin is made slowly to expand；
The crocodile skin of water, expansion is put into steaming plant by S2, boiling, and heating while is put into digestive juice, and synchronous stirring
Into its gelatinization is exercised, stop heating after being warming up to setting heat-preserving range, and kept the temperature according to setting heat preservation duration, heat preservation terminates
Stop stirring afterwards, then standing duration according to setting is stood, and a soup is formed；The boiling sets holding temperature as 75
DEG C, setting heat preservation duration ranging from 1.5 hours, setting stands duration ranging from 1.5 hours；
S3, row's soup, extract a soup, and be pumped into a soup storage device using draw-out device；
A soup in soup storage device is pumped into hydrolysis device, is warming up to setting and goes out by S4, fermentation hydrolysis
Bacterium temperature is simultaneously kept according to setting sterilizing duration, and trypsase is added and ferments, alkali protease is then added and carries out
This ferment is sent out for the second time, is cooled to setting hydrolysis temperature, and configuration liquid is added by a soup pH value adjustment to neutrality, pepsin is added
Stirring hydrolysis, and keep to enzymolysis duration is set, setting enzyme-removal temperature is warming up to after enzymolysis, and keep to when setting enzyme deactivation
It is long, form the hydrolyzate after enzyme deactivation；Set ranging from 100 DEG C of enzyme-removal temperature, setting enzyme deactivation duration ranging from 30 minutes；
S5, filtering will stand after the hydrolyzate input diatomite and/or activated carbon after the enzyme deactivation, and utilize filtering dress
Filtering is set to clear liquid storage device, then follows the clear liquid according to setting circulating filtration duration using circulation filter
Ring filters；
Clear liquid after the circulating filtration is concentrated into setting concentration concentration, forms the concentrate after clarification by S6, concentration；
The concentration sets concentration concentration range as 20 Baumes；
S7, spray drying spray the concentrate after the clarification according to setting inlet air temperature and setting leaving air temp
Mist is dried, and crocodile skin peptide finished product is formed.The spray drying sets ranging from 210 DEG C of inlet air temperature, sets leaving air temp range
It is 100 DEG C.
Embodiment 3：The extracting method of the crocodile skin peptide of the present embodiment is：
The pretreatment of S1, crocodile skin, the fresh crocodile skin chopping after slaughtering, put into processing kettle, fresh crocodile are added
The vulcanization oxygenation pretreatment of skin tare weight 7%, makes scale loosen, and in favor of the dissolving that falls off of squama, and crocodile skin is made slowly to expand；
The crocodile skin of water, expansion is put into steaming plant by S2, boiling, and heating while is put into digestive juice, and synchronous stirring
Into its gelatinization is exercised, stop heating after being warming up to setting heat-preserving range, and kept the temperature according to setting heat preservation duration, heat preservation terminates
Stop stirring afterwards, then standing duration according to setting is stood, and a soup is formed；The boiling sets holding temperature as 55
DEG C, setting heat preservation duration ranging from 1.2 hours, setting stands duration ranging from 1.3 hours；
S3, row's soup, extract a soup, and be pumped into a soup storage device using draw-out device；
A soup in soup storage device is pumped into hydrolysis device, is warming up to setting and goes out by S4, fermentation hydrolysis
Bacterium temperature is simultaneously kept according to setting sterilizing duration, and trypsase is added and ferments, alkali protease is then added and carries out
This ferment is sent out for the second time, is cooled to setting hydrolysis temperature, and configuration liquid is added by a soup pH value adjustment to neutrality, pepsin is added
Stirring hydrolysis, and keep to enzymolysis duration is set, setting enzyme-removal temperature is warming up to after enzymolysis, and keep to when setting enzyme deactivation
It is long, form the hydrolyzate after enzyme deactivation；Set ranging from 95 DEG C of enzyme-removal temperature, setting enzyme deactivation duration ranging from 20 minutes；
S5, filtering will stand after the hydrolyzate input diatomite and/or activated carbon after the enzyme deactivation, and utilize filtering dress
Filtering is set to clear liquid storage device, then follows the clear liquid according to setting circulating filtration duration using circulation filter
Ring filters；
Clear liquid after the circulating filtration is concentrated into setting concentration concentration, forms the concentrate after clarification by S6, concentration；
The concentration sets concentration concentration range as 15 Baumes；
S7, spray drying spray the concentrate after the clarification according to setting inlet air temperature and setting leaving air temp
Mist is dried, and crocodile skin peptide finished product is formed.The spray drying sets ranging from 200 DEG C of inlet air temperature, sets leaving air temp range
It is 95 DEG C.
The extracting method of the crocodile skin peptide of the present invention is simple for process, stablizes, of low cost, high income, 1, crocodile obtained
Skin peptide contains the hemoglobin of the efficient antibody and peculiar construction that are extracted out of crocodile body, can improve the immunity of the human body as quick as thought and
Blood oxygen uptake capacity can greatly improve the health quality of human body；The present invention is made crocodile skin peptide and contains strong anticarcinogen
Matter and the growth factor for preventing tumor neonatal cell, containing strong antibacterial material, containing the substance that can optimize nucleic acid, this
Kind substance keeps nucleic acid dynamic, and disease resistance is reinforced, while swashing the activity of intravital catalase and SOD, effectively removes body
Interior rubbish, containing a large amount of calcium activated and phosphorus, magnesium etc., can be used for treating senile osteoporosis, promote to retarding senility of life
The special efficacies such as upgrowth and development of children.2, crocodile skin peptide made from method using the present invention can be dissolved in rapidly without stirring
Water, aqueous solution is limpid transparent, is light yellow transparent liquid, any impurity and sediment will not be precipitated.
The present invention is compared with prior art, and technological progress is significant.Crocodile skin progress biological enzymolysis is extracted
Small-molecular peptides substance, the essential ingredient activity in crocodile skin peptide is superpower, is easily absorbed by the body, fully adjustable human immunity
Power.Product made from the method for the present invention is free of any chemical composition and hormone, does not have any toxic side effect to human body, is very pure
Net nutriment.Crocodile skin peptide made from method using the present invention can be dissolved in rapidly water without stirring, and aqueous solution is limpid
It is transparent, it is light yellow transparent liquid, any impurity and sediment will not be precipitated.To the growth of human body, development, metabolism, procreation etc.
Physiological activity has vital effect.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology can all carry out modifications and changes to above-described embodiment without violating the spirit and scope of the present invention.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should by the present invention claim be covered.
Claims (5)
1. a kind of extracting method of crocodile skin peptide, it is characterised in that：The step of extracting method is：
The pretreatment of S1, crocodile skin, the fresh crocodile skin chopping after slaughtering, put into processing kettle, fresh crocodile skin skin are added
The vulcanization oxygenation pretreatment for weighing 6~8%, makes scale loosen, and in favor of the dissolving that falls off of squama, and crocodile skin is made slowly to expand；
The crocodile skin of water, expansion is put into steaming plant by S2, boiling, and heating while is put into digestive juice, and synchronous stirring carries out
Make its gelatinization, stops heating after being warming up to setting heat-preserving range, and kept the temperature according to setting heat preservation duration, stop after heat preservation
It only stirs, then standing duration according to setting is stood, and a soup is formed；
S3, row's soup, extract a soup, and be pumped into a soup storage device using draw-out device；
A soup in soup storage device is pumped into hydrolysis device by S4, fermentation hydrolysis, is warming up to setting sterilizing temperature
It spends and is kept according to setting sterilizing duration, trypsase is added and ferments, alkali protease is then added and carries out second
Secondary this ferment of hair is cooled to setting hydrolysis temperature, and configuration liquid is added by a soup pH value adjustment to neutrality, pepsin stirring is added
Hydrolysis, and keep digesting duration to setting, setting enzyme-removal temperature is warming up to after enzymolysis, and keep to setting enzyme deactivation duration,
Form the hydrolyzate after enzyme deactivation；
S5, filtering will stand after the hydrolyzate input diatomite and/or activated carbon after the enzyme deactivation, and utilize filter device mistake
Then the clear liquid is circulated throughout using circulation filter according to setting circulating filtration duration by filter to clear liquid storage device
Filter；
Clear liquid after the circulating filtration is concentrated into setting concentration concentration, forms the concentrate after clarification by S6, concentration；
Concentrate after the clarification is carried out spraying according to setting inlet air temperature and setting leaving air temp and done by S7, spray drying
It is dry, form crocodile skin peptide finished product.
2. the extracting method of crocodile skin peptide according to claim 1, it is characterised in that：In step s 2, the boiling is set
It is 50~75 DEG C to determine holding temperature, and setting heat preservation duration ranging from 1~1.5 hour, it is small that setting stands duration ranging from 1~1.5
When.
3. the extracting method of crocodile skin peptide according to claim 1, it is characterised in that：In step s 4, enzyme deactivation temperature is set
Ranging from 90~100 DEG C of degree, setting enzyme deactivation duration ranging from 10~30 minutes.
4. the extracting method of crocodile skin peptide according to claim 1, it is characterised in that：In step s 6, the concentration is set
Surely concentration concentration range is 10~20 Baumes.
5. the extracting method of crocodile skin peptide according to claim 1, it is characterised in that：In the step s 7, the spraying is dry
Ranging from 190~210 DEG C of dry setting inlet air temperature sets ranging from 90~100 DEG C of leaving air temp.
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| CN201810641507.3A CN108753891A (en) | 2018-06-20 | 2018-06-20 | A kind of extracting method of crocodile skin peptide |
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| CN201810641507.3A CN108753891A (en) | 2018-06-20 | 2018-06-20 | A kind of extracting method of crocodile skin peptide |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731665A (en) * | 2009-12-14 | 2010-06-16 | 林峰亮 | Enzymolysis method for crocodile tissues and preparation method for crocodile wine |
| CN103421872A (en) * | 2013-03-08 | 2013-12-04 | 广州市鼍龙生物技术开发有限公司 | Preparation method and application of crocodile-skin collagen peptide |
| CN104561210A (en) * | 2015-01-27 | 2015-04-29 | 张恒 | Extraction method of deer bone peptide |
| CN104611399A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Method for extracting sea cucumber polypeptide |
| CN105886585A (en) * | 2015-01-28 | 2016-08-24 | 张恒 | Cow's penis peptide extraction method |
-
2018
- 2018-06-20 CN CN201810641507.3A patent/CN108753891A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731665A (en) * | 2009-12-14 | 2010-06-16 | 林峰亮 | Enzymolysis method for crocodile tissues and preparation method for crocodile wine |
| CN103421872A (en) * | 2013-03-08 | 2013-12-04 | 广州市鼍龙生物技术开发有限公司 | Preparation method and application of crocodile-skin collagen peptide |
| CN104561210A (en) * | 2015-01-27 | 2015-04-29 | 张恒 | Extraction method of deer bone peptide |
| CN104611399A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Method for extracting sea cucumber polypeptide |
| CN105886585A (en) * | 2015-01-28 | 2016-08-24 | 张恒 | Cow's penis peptide extraction method |
Non-Patent Citations (1)
| Title |
|---|
| 孙静: "制革生产技术问答、牛皮、杂皮制革", 《制革生产技术问答、牛皮、杂皮制革》 * |
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Effective date of registration: 20210111 Address after: 572623 No.8, north side of Hongcao village, Sanjia Town, Dongfang City, Hainan Province Applicant after: Hainan crocodile Industry Technology Co.,Ltd. Address before: 100124 2-15a-1, 15th floor, building 2, yard 2, Dajiaoting middle street, Chaoyang District, Beijing Applicant before: BAISHENGKANG (BEIJING) HEALTH INDUSTRY TECHNOLOGY Co.,Ltd. |
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Application publication date: 20181106 |
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