CN105886585A - Cow's penis peptide extraction method - Google Patents
Cow's penis peptide extraction method Download PDFInfo
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- CN105886585A CN105886585A CN201510042342.4A CN201510042342A CN105886585A CN 105886585 A CN105886585 A CN 105886585A CN 201510042342 A CN201510042342 A CN 201510042342A CN 105886585 A CN105886585 A CN 105886585A
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- pizzle
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Abstract
The invention relates to a cow's penis peptide extraction method, which comprises steps of cleaning, digesting, discharging soup, hydrolyzing, filtering, concentrating and spray-drying. With the application of the extraction method disclosed by the invention, problems on the effective absorption and utilization of macromolecular protein in human body are effectively solved, and micromolecular transformation and extraction of the protein are achieved. Rich tissue protein in cow's penis is transformed into micromolecular peptide through directional biological enzymolysis; the protein, which is derived from an animal body, is quite close to human body in structure and function, and the protein, which has a micromolecular structure, can be directly absorbed by intestines without digestion and can rapidly participate in normal metabolism and regulation of the human body; therefore, the protein, in pharmacology, is higher in biochemical mechanism reasonability and specific treatment effectiveness. The cow's penis peptide has the characteristics of being strongly targeted, free from toxic and side effects, significant in curative effect, high in nutritive value, easy to to be absorbed by human body and the like.
Description
Technical field
The present invention relates to bioengineering field, particularly to a kind of method extracting small-molecular peptides from pizzle.
Background technology
The collagen content of pizzle is up to 98% rich in androgen, protein, fat, kidney tonifying to hold up sun,
Cure mainly the diseases such as impotence due to the kidney deficiency, seminal emission, soreness and weakness of waist and knees.Modern study proves, animal viscera at biochemical characteristic and
Also there is many and people's similarity in constituent structure, and at aspects such as physiological functions, there is many similar spies
Point, therefore organ-tissue corresponding to human body has pharmacological action, and this is " dirty with dirty benefit " theory.
Product with pizzle as raw material is few the most on the market, is mostly and cooks.And directly eat pizzle essence
On can not change the macromolecular structure of the original protein of pizzle, such product is as a kind of macromolecular
Compound is difficult to be absorbed and utilized by the body.
Summary of the invention
In order to solve how prior art allows high molecular weight protein how effectively be absorbed by human body and asking of utilizing
Topic, carries out conversion and the extraction of little molecule to albumen, and the present invention provides a kind of pizzle peptide extracting method.Described
Technical scheme is as follows:
A kind of pizzle peptide extracting method, comprises the following steps:
Cleaning step, is proportionally added into clear water, alimentary acetic acid and pizzle, by pizzle according to setting pickling duration
Carry out pickling, be carried out then according to set scavenging period, form the pizzle after cleaning；
Boiling step, puts into steaming plant by water, puts into alimentary acetic acid and the meter described pizzle of weight while intensification,
And synchronize to be stirred, stop heating up after being warming up to set heat-preserving range, and carry out according to setting insulation duration
Insulation, insulation stops stirring after terminating, and stands then according to setting stands duration, forms a soup；
Row's soup step, utilizes draw-out device to extract a described soup, and pumps into a soup storage device；
Hydrolysing step, pumps in hydrolysis device by a soup in a described soup storage device, is warming up to set
Determining sterilising temp and keep according to setting sterilizing duration, being then cooled to set hydrolysis temperature, addition is joined
Put liquid by a soup pH value regulation to neutral, put into animal protein enzyme preparation and be hydrolyzed, and keep to setting
Enzymolysis duration, enzymolysis is warming up to set enzyme-removal temperature, and keeps to setting the enzyme duration that goes out after terminating, formation is gone out
Hydrolyzate after enzyme；
Filtration step, stands after the hydrolyzate after the described enzyme that goes out puts into diatomite and/or activated carbon, and utilizes
Filter filters to clear liquid storage device, then utilizes circulation filter to follow according to setting described clear liquid
Ring filters duration and is circulated filtration；
Concentration step, is concentrated into the clear liquid after described circulating filtration setting and concentrates concentration, after forming clarification
Concentrate；
Spray drying step, by the concentrate after described clarification according to setting EAT and setting leaving air temp
It is spray-dried, forms pizzle peptide pulvis.
In cleaning step, set pickling duration scope as 0.5～1.5 hour, set when cleaning a length of 5～15
Minute.
In boiling step, set heat-preserving range as 55～85 DEG C, set insulation duration scope little as 0.5～1.5
Time, set standing duration scope as 0.5～1.5 hour.
In row's soup step, draw-out device is specially vacuum extraction tank.
In hydrolysing step, set sterilising temp scope as 90～110 DEG C, set sterilizing duration scope as 5～20
Minute, setting hydrolysis temperature scope as 55～75 DEG C, configuration liquid is specially NaoH, sets enzymolysis duration scope
It is 4～6 hours, sets enzyme-removal temperature scope as 80～100 DEG C, set enzyme duration scope of going out as 5～25 minutes.
In filtration step, filter is specially tub filter and/or two-stage plate filter, is circulated throughout
Filter device is specially plate filter, sets circulating filtration duration scope as 1～3 hour.
In concentration step, set the concentration concentration range concentrate need profit again as 10～20 Baumes, to muddiness
Carry out filtering or utilize tube centrifuge to separate with plate filter, the concentrate of described muddiness is formed
The concentrate of clarification.
In spray drying step, set EAT scope as 190～210 DEG C, set leaving air temp scope as
90-100℃。
The present invention efficiently solves the problem how high molecular weight protein is effectively absorbed by human body and utilize, to albumen
Carry out conversion and the extraction of little molecule.The biological extractive technique of advanced person by safety, by pizzle, it enriches
Histone become small-molecular peptides through bio-orientation enzymolysis, owing to protein is from animal body, its structure and
Closely, and molecule is little for function and human body, it is not necessary to digestion, can directly be absorbed by enteron aisle, fast join
With eubolism and the regulation of human body, pharmacologically, there is higher biochemical mechanism reasonability and special control
Treat validity.Pizzle peptide have with strong points, have no side effect, evident in efficacy, be of high nutritive value, easy quilt
The features such as absorption of human body.
The pizzle peptide of the present invention does not contains any chemical composition and hormone, does not has any toxic and side effect to human body, is
The purest nutriment.Without stirring can be dissolved in rapidly water, the aqueous solution is limpid transparent, for white or
Light yellow transparent liquid, will not separate out any impurity and sediment.
Pizzle peptide molecular weight is distributed in below 1000 dalton, and activity is superpower, easily by human body or percutaneous absorption,
Can fast participate in cellular physiological events, male sex respective organization organotrophy balance can be met to a greater degree
Demand.Rich amino acids and the most fatty in pizzle peptide, is not required to digest directly absorption；Biologically active is strong
Can absorbed intact；Uncompetitive active absorption；Consuming energy low does not increase stomach burden.Pizzle peptide can promote cell
Blood circulation, promotes the secretion of male sex hormone, the property improved function.There is the effect of anti-inflammation, can resist
Venereal disease, the infection of AIDS virus.According to the principle of theory of traditional Chinese medical science " dirty with dirty benefit, People of the same tastes and habits like to be together ", this is dirty
Device peptide has good benefiting action to human body corresponding organs, makes the Special food that people cannot directly eat
Nutritional labeling is supplemented.Be suitable for hypoimmunity, suffer from a deficiency of the kidney, sex dysfunction, fatigable person in middle and old age
The male sex.
It should be appreciated that it is only exemplary that above general description and details hereinafter describe, can not
Limit the present invention.
Accompanying drawing explanation
Accompanying drawing herein is merged in specification and constitutes the part of this specification, it is shown that meet the present invention
Embodiment, and in specification together for explaining the principle of the present invention.
Fig. 1 is the method flow schematic diagram of the present invention；
Fig. 2 is the method flow schematic diagram of the embodiment of the present invention.
Detailed description of the invention
Here will illustrate exemplary embodiment in detail, its example represents in the accompanying drawings.Following retouches
Stating when relating to accompanying drawing, unless otherwise indicated, the same numbers in different accompanying drawings represents same or analogous key element.
Embodiment described in following exemplary embodiment does not represent all embodiment party consistent with the present invention
Formula.On the contrary, they only with describe in detail in appended claims, the present invention some in terms of mutually one
The example of the apparatus and method caused.
Fig. 1 is the method according to the invention schematic flow sheet, relates to a kind of pizzle peptide extracting method, including with
Lower step:
Cleaning step 101: be proportionally added into clear water, alimentary acetic acid and pizzle, by pizzle according to when setting pickling
Length carries out pickling, is carried out then according to set scavenging period, forms the pizzle after cleaning.Set pickling
Duration scope is 0.5～1.5 hour, set clean time a length of 5～15 minutes.
Boiling step 102: water is put into steaming plant, puts into alimentary acetic acid and the meter described ox of weight while intensification
Whip, and synchronize to be stirred, stop heating up after being warming up to set heat-preserving range, and according to setting insulation duration
Being incubated, insulation stops stirring after terminating, and stands then according to set standing duration, is formed once
Soup.Set heat-preserving range as 55～85 DEG C, set insulation duration scope as 0.5～1.5 hour, set when standing
Long scope is 0.5～1.5 hour.
Row's soup step 103: utilize draw-out device to extract a described soup, and pump into a soup storage device.Take out
Fetching puts specially vacuum extraction tank.
Hydrolysing step 104 a: soup in a described soup storage device is pumped in hydrolysis device, is warming up to
Set sterilising temp and keep according to setting sterilizing duration, being then cooled to set hydrolysis temperature, add
Soup pH value regulation to neutral, put into animal protein enzyme preparation and is hydrolyzed, and keep to setting by configuration liquid
Determining enzymolysis duration, enzymolysis is warming up to set enzyme-removal temperature after terminating, and keeps to setting the enzyme duration that goes out, and is formed
Go out the hydrolyzate after enzyme.Set sterilising temp scope as 90～110 DEG C, set sterilizing duration scope as 5～20
Minute, setting hydrolysis temperature scope as 55～75 DEG C, configuration liquid is specially NaoH, sets enzymolysis duration scope
It is 4～6 hours, sets enzyme-removal temperature scope as 80～100 DEG C, set enzyme duration scope of going out as 5～25 minutes.
Filtration step 105: stand after the hydrolyzate after the described enzyme that goes out is put into diatomite and/or activated carbon, and
Utilize filter to filter to clear liquid storage device, then utilize circulation filter according to setting described clear liquid
Determine circulating filtration duration and be circulated filtration.Filter is specially tub filter and/or two-stage plate-frame filtering
Device, circulation filter is specially plate filter, sets circulating filtration duration scope as 1～3 hour.
Concentration step 106: the clear liquid after described circulating filtration is concentrated into setting and concentrates concentration, after forming clarification
Concentrate.Set concentration concentration range as 10～20 Baumes, muddy concentrate need to be recycled sheet frame mistake
Filter carries out filtering or utilize tube centrifuge to separate, and the concentrate of described muddiness is formed the dense of clarification
Contracting liquid.
Spray drying step 107: by the concentrate after described clarification according to setting EAT and setting out wind-warm syndrome
Degree is spray-dried, and forms pizzle peptide pulvis.Set EAT scope as 190～210 DEG C, set air-out
Temperature range is 90-100 DEG C.
Fig. 2 is the method flow schematic diagram of the embodiment that the present invention provides, and relates to a kind of pizzle peptide extracting method,
Comprise the following steps:
Cleaning step 201: be proportionally added into clear water, alimentary acetic acid and pizzle, is carried out pizzle according to 1 hour
Pickling, was carried out according to 10 minutes, formed the pizzle after cleaning.
Boiling step 202: water is put into steaming plant, puts into alimentary acetic acid and the meter described ox of weight while intensification
Whip, and synchronize to be stirred, stop after being warming up to 65～75 DEG C heating up, and be incubated according to 1 hour, protect
Temperature stops stirring after terminating, then according to 1 hour stands, forms a soup.
Row's soup step 203: utilize vacuum extraction tank to extract a described soup, and pump into a soup storage device.
Hydrolysing step 204 a: soup in a described soup storage device is pumped in hydrolysis device, is warming up to
98～100 DEG C and kept according to 10～15 minutes, being then cooled to 60～65 DEG C, adding NaoH will once
The regulation of soup pH value, to neutral, put into animal protein enzyme preparation and is hydrolyzed, and keep to 5 hours, and enzymolysis is tied
It is warming up to 90 DEG C after bundle, and keeps to 15 minutes, form the hydrolyzate after the enzyme that goes out.
Filtration step 205: stand after the hydrolyzate after the described enzyme that goes out is put into diatomite and/or activated carbon, and
Tub filter and/or two-stage plate filter is utilized to filter to clear liquid storage device, then by described clear liquid profit
It was circulated filtration according to 2 hours with plate filter.
Concentration step 206: the clear liquid after described circulating filtration is concentrated into 13～15 Baumes, after forming clarification
Concentrate.
Spray drying step 207: by the concentrate after described clarification according to EAT 200 DEG C and leaving air temp
93～95 DEG C are spray-dried, and form pizzle peptide pulvis.
The present invention efficiently solves the problem how high molecular weight protein is effectively absorbed by human body and utilize, to albumen
Carry out conversion and the extraction of little molecule.The biological extractive technique of advanced person by safety, by pizzle, it enriches
Histone become small-molecular peptides through bio-orientation enzymolysis, owing to protein is from animal body, its structure and
Closely, and molecule is little for function and human body, it is not necessary to digestion, can directly be absorbed by enteron aisle, fast join
With eubolism and the regulation of human body, pharmacologically, there is higher biochemical mechanism reasonability and special control
Treat validity.Pizzle peptide have with strong points, have no side effect, evident in efficacy, be of high nutritive value, easy quilt
The features such as absorption of human body.
The pizzle peptide of the present invention does not contains any chemical composition and hormone, does not has any toxic and side effect to human body, is
The purest nutriment.Without stirring can be dissolved in rapidly water, the aqueous solution is limpid transparent, for white or
Light yellow transparent liquid, will not separate out any impurity and sediment.
Pizzle peptide molecular weight is distributed in below 1000 dalton, and activity is superpower, easily by human body or percutaneous absorption,
Can fast participate in cellular physiological events, male sex respective organization organotrophy balance can be met to a greater degree
Demand.Rich amino acids and the most fatty in pizzle peptide, is not required to digest directly absorption；Biologically active is strong
Can absorbed intact；Uncompetitive active absorption；Consuming energy low does not increase stomach burden.Pizzle peptide can promote cell
Blood circulation, promotes the secretion of male sex hormone, the property improved function.There is the effect of anti-inflammation, can resist
Venereal disease, the infection of AIDS virus.According to the principle of theory of traditional Chinese medical science " dirty with dirty benefit, People of the same tastes and habits like to be together ", this is dirty
Device peptide has good benefiting action to human body corresponding organs, makes the Special food that people cannot directly eat
Nutritional labeling is supplemented.Be suitable for hypoimmunity, suffer from a deficiency of the kidney, sex dysfunction, fatigable person in middle and old age
The male sex.
Those skilled in the art, after considering specification and putting into practice invention disclosed herein, will readily occur to this
Other bright embodiment.The application is intended to any modification, purposes or the adaptations of the present invention,
These modification, purposes or adaptations are followed the general principle of the present invention and include that the present invention is not disclosed
Common knowledge in the art or conventional techniques means.Description and embodiments is considered only as exemplary
, true scope and spirit of the invention are pointed out by claim below.It should be appreciated that the present invention
It is not limited to precision architecture described above and illustrated in the accompanying drawings, and can be without departing from its model
Enclose and carry out various modifications and changes.The scope of the present invention is only limited by appended claim.
Claims (10)
1. a pizzle peptide extracting method, it is characterised in that said method comprising the steps of:
Cleaning step, is proportionally added into clear water, alimentary acetic acid and pizzle, by pizzle according to setting pickling duration
Carry out pickling, be carried out then according to set scavenging period, form the pizzle after cleaning；
Boiling step, puts into steaming plant by water, puts into alimentary acetic acid and the meter described pizzle of weight while intensification,
And synchronize to be stirred, stop heating up after being warming up to set heat-preserving range, and carry out according to setting insulation duration
Insulation, insulation stops stirring after terminating, and stands then according to setting stands duration, forms a soup；
Row's soup step, utilizes draw-out device to extract a described soup, and pumps into a soup storage device；
Hydrolysing step, pumps in hydrolysis device by a soup in a described soup storage device, is warming up to set
Determining sterilising temp and keep according to setting sterilizing duration, being then cooled to set hydrolysis temperature, addition is joined
Put liquid by a soup pH value regulation to neutral, put into animal protein enzyme preparation and be hydrolyzed, and keep to setting
Enzymolysis duration, enzymolysis is warming up to set enzyme-removal temperature, and keeps to setting the enzyme duration that goes out after terminating, formation is gone out
Hydrolyzate after enzyme；
Filtration step, stands after the hydrolyzate after the described enzyme that goes out puts into diatomite and/or activated carbon, and utilizes
Filter filters to clear liquid storage device, then utilizes circulation filter to follow according to setting described clear liquid
Ring filters duration and is circulated filtration；
Concentration step, is concentrated into the clear liquid after described circulating filtration setting and concentrates concentration, after forming clarification
Concentrate；
Spray drying step, by the concentrate after described clarification according to setting EAT and setting leaving air temp
It is spray-dried, forms pizzle peptide pulvis.
A kind of pizzle peptide extracting method the most according to claim 1, it is characterised in that described cleaning walks
The pickling duration scope that sets in Zhou as 0.5～1.5 hour, set clean time a length of 5～15 minutes.
A kind of pizzle peptide extracting method the most according to claim 1, it is characterised in that described boiling walks
Set heat-preserving range as 55～85 DEG C in Zhou, set insulation duration scope as 0.5～1.5 hour.
A kind of pizzle peptide extracting method the most according to claim 1, it is characterised in that described row's soup walks
Draw-out device in Zhou is specially vacuum extraction tank.
A kind of pizzle peptide extracting method the most according to claim 1, it is characterised in that described hydrolysis step
The sterilising temp scope that sets in Zhou, as 90～110 DEG C, sets sterilizing duration scope as 5～20 minutes.
A kind of pizzle peptide extracting method the most according to claim 1, it is characterised in that described hydrolysis step
The hydrolysis temperature scope that sets in Zhou, as 55～75 DEG C, sets enzymolysis duration scope as 4～6 hours.
A kind of pizzle peptide extracting method the most according to claim 1, it is characterised in that described hydrolysis step
The enzyme-removal temperature scope that sets in Zhou, as 80～100 DEG C, sets enzyme duration scope of going out as 5～25 minutes.
A kind of pizzle peptide extracting method the most according to claim 1, it is characterised in that described filtration walks
Filter in Zhou is specially tub filter and/or two-stage plate filter.
A kind of pizzle peptide extracting method the most according to claim 1, it is characterised in that described concentration walks
Setting in Zhou concentrates concentration range as 10～20 Baumes.
A kind of pizzle peptide Advanced method the most according to claim 1, it is characterised in that described spraying
The EAT scope that sets in drying steps, as 190～210 DEG C, sets leaving air temp scope as 90-100 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510042342.4A CN105886585A (en) | 2015-01-28 | 2015-01-28 | Cow's penis peptide extraction method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510042342.4A CN105886585A (en) | 2015-01-28 | 2015-01-28 | Cow's penis peptide extraction method |
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| CN105886585A true CN105886585A (en) | 2016-08-24 |
Family
ID=57002056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510042342.4A Pending CN105886585A (en) | 2015-01-28 | 2015-01-28 | Cow's penis peptide extraction method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755246A (en) * | 2016-12-13 | 2017-05-31 | 国肽生物科技(北京)有限公司 | The extracting method of thymic peptide |
| CN108753890A (en) * | 2018-06-20 | 2018-11-06 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile whip peptide |
| CN108753889A (en) * | 2018-06-20 | 2018-11-06 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile courage peptide |
| CN108753891A (en) * | 2018-06-20 | 2018-11-06 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile skin peptide |
| CN113388477A (en) * | 2021-05-26 | 2021-09-14 | 杭州医学院 | Sheep penis polypeptide wine and its production method |
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| CN101249100A (en) * | 2008-03-27 | 2008-08-27 | 阿坝州九寨鼎盛科技有限责任公司 | Compound formula to supplement protein and manufacture method thereof |
| CN102367465A (en) * | 2011-10-18 | 2012-03-07 | 得利斯集团有限公司 | Extraction method of pig blood protein peptide |
| CN104450840A (en) * | 2014-11-14 | 2015-03-25 | 四川大学 | Production process of bovine bone peptone |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101249100A (en) * | 2008-03-27 | 2008-08-27 | 阿坝州九寨鼎盛科技有限责任公司 | Compound formula to supplement protein and manufacture method thereof |
| CN102367465A (en) * | 2011-10-18 | 2012-03-07 | 得利斯集团有限公司 | Extraction method of pig blood protein peptide |
| CN104450840A (en) * | 2014-11-14 | 2015-03-25 | 四川大学 | Production process of bovine bone peptone |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755246A (en) * | 2016-12-13 | 2017-05-31 | 国肽生物科技(北京)有限公司 | The extracting method of thymic peptide |
| CN108753890A (en) * | 2018-06-20 | 2018-11-06 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile whip peptide |
| CN108753889A (en) * | 2018-06-20 | 2018-11-06 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile courage peptide |
| CN108753891A (en) * | 2018-06-20 | 2018-11-06 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile skin peptide |
| CN113388477A (en) * | 2021-05-26 | 2021-09-14 | 杭州医学院 | Sheep penis polypeptide wine and its production method |
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Application publication date: 20160824 |