CN101724686B - 检测水产品中重要致病菌的基因芯片和试剂盒 - Google Patents

检测水产品中重要致病菌的基因芯片和试剂盒 Download PDF

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CN101724686B
CN101724686B CN2008101723981A CN200810172398A CN101724686B CN 101724686 B CN101724686 B CN 101724686B CN 2008101723981 A CN2008101723981 A CN 2008101723981A CN 200810172398 A CN200810172398 A CN 200810172398A CN 101724686 B CN101724686 B CN 101724686B
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seq
dna
probe
gene chip
proteus
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CN101724686A (zh
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王磊
刘蕾
曹勃阳
王敏
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Tianjin Biochip Corp
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Tianjin Biochip Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2008101723981A 2008-11-03 2008-11-03 检测水产品中重要致病菌的基因芯片和试剂盒 Expired - Fee Related CN101724686B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2008101723981A CN101724686B (zh) 2008-11-03 2008-11-03 检测水产品中重要致病菌的基因芯片和试剂盒
PCT/CN2009/001190 WO2010060262A1 (fr) 2008-11-03 2009-10-26 Puce à adn et kit pour la détection d'une bactérie pathogène importante dans des produits aquatiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2008101723981A CN101724686B (zh) 2008-11-03 2008-11-03 检测水产品中重要致病菌的基因芯片和试剂盒

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CN101724686A CN101724686A (zh) 2010-06-09
CN101724686B true CN101724686B (zh) 2012-02-29

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CN (1) CN101724686B (fr)
WO (1) WO2010060262A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102311993A (zh) * 2010-07-08 2012-01-11 天津生物芯片技术有限责任公司 用于检测水产品中重要致病菌的基因芯片及其试剂盒
CN102140507B (zh) * 2010-12-20 2012-12-26 南开大学 用于感染性腹泻的检测型基因芯片及检测用试剂盒
CN102140515B (zh) * 2011-01-04 2012-12-12 天津生物芯片技术有限责任公司 用于检测水产品中重要致病菌的核苷酸及其应用
CN103540668A (zh) * 2013-10-22 2014-01-29 宁波大学 检测10种海域致病菌的基因芯片
CN105133040B (zh) * 2015-06-30 2018-01-23 宁波大学 一种检测海洋致病弧菌的基因芯片及其制备方法与检测方法
CN109852674B (zh) * 2019-01-23 2022-08-23 浙江工商大学 基于随机扩增标记和原位合成微流体芯片的水产病原微生物检测方法
CN110951895B (zh) * 2019-12-24 2021-03-23 重庆市畜牧科学院 检测和区分奇异变形杆菌、普通变形杆菌和潘氏变形杆菌的系统和方法
CN112011448B (zh) * 2020-07-20 2023-04-11 深圳市刚竹医疗科技有限公司 微流控芯片与试剂盒及其应用方法
CN114934045B (zh) * 2022-06-06 2024-06-21 福建省长汀盼盼食品有限公司 一种食品微生物检测的探针、芯片、试剂盒及方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1536090A (zh) * 2003-04-07 2004-10-13 中国人民解放军军事医学科学院卫生学 食源性致病菌快速检测基因芯片及其应用
CN101045944B (zh) * 2007-01-12 2010-12-22 北京爱普益生物科技有限公司 检测六种腹泻致病菌的基因芯片、制备方法及试剂盒
CN101240335B (zh) * 2007-02-09 2011-02-02 天津生物芯片技术有限责任公司 检测奶制品中常见致病菌的基因芯片和试剂盒
CN101113476B (zh) * 2007-05-30 2010-05-26 中国疾病预防控制中心传染病预防控制所 一种病原微生物dna检测芯片及其制备方法和应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
侯晓丽.菌血症及肠道感染常见病原菌高通量基因诊断研究.《浙江大学博士学位论文》.2006,全文. *
杨文鸽等.水产品中致病微生物的快速检测方法.《中国食品学报》.2006,第6卷(第1期),402-406. *

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WO2010060262A1 (fr) 2010-06-03

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