CN101723763B - Soybean biological seed coating agent - Google Patents

Soybean biological seed coating agent Download PDF

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Publication number
CN101723763B
CN101723763B CN2009100731294A CN200910073129A CN101723763B CN 101723763 B CN101723763 B CN 101723763B CN 2009100731294 A CN2009100731294 A CN 2009100731294A CN 200910073129 A CN200910073129 A CN 200910073129A CN 101723763 B CN101723763 B CN 101723763B
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agent
solution
water
soybean
seed
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CN101723763A (en
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冯乃杰
郑殿峰
孙冬梅
张春娟
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HEILONGJIANG AUGUST FIRST LAND RECLAMATION UNIVERSITY
Heilongjiang Bayi Agricultural University
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HEILONGJIANG AUGUST FIRST LAND RECLAMATION UNIVERSITY
Heilongjiang Bayi Agricultural University
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Abstract

The invention discloses a soybean biological seed coating agent which is prepared from the following components in percentage by weight: 20 percent of complex microbial community, 5 percent of bio-regulator SODM, 10 percent of trace element, 10 percent of auxiliary agent, 0.4 percent of total matrines, and the balance of water. The invention has the advantages of environmental protection, enhancement of crop stress tolerance, strengthening of seedling, and improvement of yield and quality.

Description

Soybean biological seed coating agent
Technical field:
The present invention relates to a kind of soya seeds inorganic agent, especially a kind of new and effective seed pelleting product take bio-active bacteria (fungi) as active ingredient.
Background technology:
Seed coating technology is a seed New Machining Technology that grows up on the basis of tradition seed soaking, seed dressing technology, makes seed treatment convenient, safe and effective.Plant the clothing agent as a kind of novel agrochemical on the international market, meet the requirement of modern agricultural development.Plant the clothing agent and formed by effective ingredient, binding agent and other processing aid, can directly or after dilution be wrapped in the surface of the seed, form the pesticidal preparations with some strength and permeability diaphragm.Seed treatment also has the history of centuries abroad, and as far back as the 6's of 19th century, the Blession of the U.S. just proposes with batter to the cotton seeds dressing, and purpose makes the large granulation of cotton seed, homogenising, is convenient to sowing, and is after this slow to kind of the progress of clothing agent.Until nineteen twenty-six the U.S. Thornton and Ganulee the seed pelleting problem is at first proposed; The thirties in 20th century, the Germain seeds company of Britain successfully develops the seed coating medicine that is used as seed pelleting first on cereal crop, and from then on seed pelleting has entered the fast development phase.The initial stage eighties, the former Soviet Union developed by polymeric set compound adhesive agent, and the composite kind clothing agent that forms of plant growth regulator and triazole phenol copper makes seed coating technology produce a leap.At present, the seed coating technology of agriculture developed country and degree are all very high in the world.Capsuled seed develops into soybean, peanut, corn, water paddy and wheat class, millet, vegetables, flowers etc. from cotton, kind of clothing agent technology has become at present high in technological content a, remarkable in economical benefits, can drive the comprehensive new and high technology that whole seed industry develops in a healthy way, obtain the attention of developed country, promoting fast the development of cosmopolitan species industry.
China plants research and the application of clothing agent and starts late, use kind of a clothing agent seed coating technology since the eighties in 20th century, at present, the application of seed coating technology on cereal crops, economic crops, flowers, herbage and rare forest expanded just rapidly, also is the important breakthrough that China's " seed engineering " is obtaining in recent years.
The kind clothing agent of in the past using, it is several to be divided into the agent of single agricultural chemicals type kind clothing, composite seed coating agent, Bio-seed Coating agent etc. according to the difference of active component.Add some desinsection, bactericide, weed killer herbicide in the single agricultural chemicals type kind clothing agent dressing as kind of a clothing additive, this is kind clothing agent the most frequently used, that use is maximum.Add the various active composition in the composite seed coating agent, mainly contain: agricultural chemicals, little fertilizer and plant growth regulator, phosphorus decomposing, solution nitrogen and silicate-dissolving microbe, antibiotic etc.Can prevent the soil-borne disease bacterium infect with soil in the harm of insect.Bio-seed Coating agent applying biological control agent in kind of clothing agent along with seed; according to antagonism principle between the different biologies; screen the breeding, infringement of the harmful germ of useful microorganism antagonism and reach the purpose of diseases prevention; the environmental pollution that can avoid chemical pesticide to cause reaches the adverse effect that non-target organism, some beneficial organisms are produced, thus the protection ecological balance.In addition, also useful animals and plants extract, the kind clothing agent that inorganic mineral such as alkaloid, aromatic oil, tannin, vitamin, growth regulator, trace element, natural macromolecular material and the configuration of biological bacteriostatic agent isoreactivity material form, pollution-free to people, animal, crop and environment low toxicity.Analysis-by-synthesis can find out, plant from now on clothing agent technology development will towards safe and efficient, low toxicity is nontoxic, multi-functional compound future development.
The Soybean Seed-coating of using at present, many products have serious murder by poisoning and pollution to human body and environment, for example, it is large, seriously polluted to sell more Soybean Seed-coating toxicity such as " anti-continuous cropping, carbofurans " on the market, wherein carbofuran 7mg just can put the people in the fatal position, and in environment, stay the persistence pollution, and can not eliminate at least 50 years, this has become a large hidden danger of China's ecotype agriculture.Although the Soybean Seed-coating product is more, but in application, show many unsatisfactory parts, for example some product composition is single, function is unilateral, high malicious high residue, action effect are not obvious, although occurred in recent years some biological seed coating agent products on the market, solved to a certain extent the high malicious high residue problem of planting the clothing agent, but there are many Related products not obvious at aspect action effects such as increase output, regulation and control plant growth, diseases prevention insect protected, restricted spread and the application of the agent of these kinds clothing.
Summary of the invention:
In order to overcome the deficiency in the background technology, the invention provides a kind of soybean biological seed coating agent.This product has environmental protection, enhancing crop anti-adversity ability, strong sprout, raising output and improves the advantage of quality.
Environmental protection is followed in the design of this kind of clothing agent prescription, inexpensive, of fine quality, high yield four large principles, reducing under kind of the prerequisite of clothing agent cost of material, design non-harmful kind of clothing agent prescription, again homemade kind of clothing agent and tradition are generally acknowledged that planting preferably the clothing agent carries out Cotton seeds and carry out the on year-on-year basis experiment of various performance parameters soya seeds respectively, then the prescription that Specifeca tion speeification is surpassed the agent of tradition kind clothing is just elected, the adjustment optimization of filling a prescription on its basis again, then carry out the on year-on-year basis experiment of various performance parameters with the kind clothing agent prescription of adjusting after optimizing again, so through repeatedly adjustment, optimize, experiment determines that finally cost performance and environmental friendliness performance all are better than the best kind clothing agent prescription that tradition is planted the clothing agent.
The technical solution adopted in the present invention is: this soybean biological seed coating agent comprises following component, and each component is prepared by mass percentage: complex micro organism fungicide accounts for 20%, biological regulator SOD MAccount for 5%, trace element solution accounts for 10%, and auxiliary agent accounts for 10%, and Banlangen accounts for 0.4%, and surplus is water.
Above-mentioned complex micro organism fungicide is comprised of rhizobium solution 10%, phosphate solubilizing bacteria solution 20%, potassium solubilizing bacteria solution 20%, biocontrol microorganisms solution 20%, degradation bacteria solution 10%, short living bacterium solution 20%, each component is prepared by mass percentage, and the quantity of each effective bacterium is 10 in the above-mentioned complex micro organism fungicide 8Individual/more than the mg; Trace element solution is comprised of water, boric acid and ammonium molybdate, and water, boric acid and ammonium molybdate shared mass percent in trace element is followed successively by 99.6%, 0.2%, 0.2%; Auxiliary agent is by water, urea, polyvinyl alcohol 1788, gibberellin GA 3, sodium carboxymethylcellulose and peregal 0-25 form water, urea, polyvinyl alcohol 1788, gibberellin GA 3, sodium carboxymethylcellulose and peregal 0-25 shared mass percent in auxiliary agent be respectively: 77.95%, 10%, 10%, 0.05%, 1%, 1%.
Used the kind clothing agent aid of safety non-toxic evil effect in the technique scheme, this auxiliary agent has slow-release function, can prolong kind of the action period of clothing agent, and its film forming and dressing intensity reach the like product standard.
Used complex microorganism colony in the technique scheme: contain the very strong bactericide of some toxic actions in the kind clothing agent of in the past using, on the basis of improving original kind of clothing agent prescription, find by test of many times, the biocontrol microorganisms of gemma phase, degradation bacteria, short living bacterium, phosphate solubilizing bacteria and potassium solubilizing bacteria can survive in kind of clothing agent, dressing after planting can be bred in soil, is conducive to set up in soil beneficial microbe colony.Simultaneously, use rhizobium in the complex microorganism colony, can strengthen the nitrogen fixing capacity of root system, reduce the consumption of chemical fertilizer, be beneficial to the formation of soybean yields.
In kind of clothing agent, added first the active substance superoxide dismutase mimics of aminoacid small peptide class in the technique scheme (referred to as SOD M, be a kind of biological regulator, the patent No. 200510132464), by adding SOD M, can increase photosynthesis duration of soybean leaves, the aging of Retarding Soybean Leaf is conducive to the synthetic and accumulation of late growth stage seed internal substance.By the adding of trace element molybdenum, boron, can replenish the nutrition of soybean plant strain, improve emergence rate.
Technique scheme has overcome the in the past shortcoming of many chemical seed clothing agent height poison, high residue.Planted in the past and often contain the toxic components such as Furadan, carbendazim, tmtd in the clothing agent, total content is about 30%.Insecticide matrine by the biocontrol microorganisms in the using microbe complex and plant resource, in conjunction with slow release method, the damage by disease and insect such as Soybean Seedling Cyst nematode, root rot have all been played good preventive effect, greatly reduce kind of the toxicity of clothing agent, reduce environmental pollution, increased environmental benefit.
This product with nontoxic microorganism compound population as bactericide, plant extracts matrine with low toxicity is made insecticide, replaced to plant all has the hypertoxic raw materials such as carbofuran of severe toxicity effect to human body and environment in the clothing agent in the past, is made into novel biological seed coating agent with biological regulator, trace element, auxiliary agent etc. are compound.By the adding of complex microorganism colony, can change soil microflora, promote the beneficial microorganism growth and suppress the breeding of some phytopathogens; By the adding of biological regulator and trace element, for the soybean kernel sprouting provides nutrition, be conducive to strong sprout; After under the seed of dressing, plant clothing agent chance water imbibition but hardly dissolving, and form a barrier on every side at seed, along with Seed sprouting, germination, seedling, active ingredient slowly discharges in order, and is transmitted to the seedling each several part by Root Absorption, and medicine, fertilizer are fully used, to strengthen seed and seedling to the resistance of germ and damage by disease and insect, reach the purpose of cost-saving synergistic.
Usage of the present invention: dress seed according to seed weight 1.5%, dry in the shade behind the mixing and can sow, will avoid the sun direct projection during dressing, this product can not use simultaneously with bactericide.
The present invention has following beneficial effect: this soybean biological seed coating agent is the High Tech Products that collection seed pelleting, microbial manure, trace element and biological regulator four large key technologies are melted into a whole, realize simultaneously multi-efficiency, solved on the agricultural in the past can't integrated four technology a difficult problem.In this product, contain the biotype auxiliary agent, efficient patented product superoxide dismutase mimics SOD is arranged MThe microbial activity compositions such as phosphorus decomposing, potassium decomposing, short life, biological control and rhizobium are arranged, be aided with the components such as trace element and biological insecticides, by the soybean biological seed coating agent product of the compound novel high-efficiency environment friendly of above-mentioned each component, compare with traditional Soybean Seed-coating, both saved cost, improved again soybean yields, and nontoxic to people and animals, when improving grain yield, environment is not produced again and pollute, have obvious economy and environmental benefit.
Main application of the present invention: the control seedling stage diseases and pests guarantees soybean germination rate, the latent root fly of control, first generation cyst roundworm, aphid, root rot, thrips and paraluperodes suturalis nigrogobilineatus; Replenish the trace elements such as molybdenum, boron, regulate soybean growth, strengthen the resistance of soybean plant strain, improve the availability of soil and fertilizer, improve output.The stability of this kind clothing agent, film forming and dressing intensity reach the standard of domestic like product.Find that through strange land small plot experiment and large-scale demonstration environment-friendly type soybean biological seed coating agent product can be regulated the soybean growth process, regulate yield traits, on average improve soybean yields 22.06%.
Description of drawings:
Fig. 1 is preparation process figure of the present invention.
Embodiment:
The invention will be further described below in conjunction with embodiment:
The agent of this kind clothing is on the impact of yield traits and output:
By finding out (as shown in table 1) to the investigation of different regions output index of correlation and mensuration, biological seed coating agent has certain regulating and controlling effect to main characters of soybean and output, and the agent of applying biological kind clothing has increased single-strain grain number, 100-grain weight and the output of soybean.With compare, the soybean yields on Lindian, Heilongjiang Province Experimental Base and 852 farms, Baoqing County respectively contrast increased that 22.17% and 21.94%, two area is average to increase soybean yields 22.06%.
Biological seed coating agent is on the table 1 that affects of main characters of soybean and output
Other effect:
Separate to cultivate through indoor microorganism and detect, the soybean biological seed coating agent product stability is good, and product preserves that its micro organism quantity still reaches 10 after 1 year 8More than.Find that by field trial soybean biological seed coating agent can be regulated the growth and development process of soybean.Compared with the control without significant difference, stem has roughly increase through the soybean plant strain plant height of biological seed coating agent Cotton seeds, and root system contrasts prosperity, and root nodule numbers and weight increase to some extent, have strengthened the lodging resistance of soybean plant strain.Simultaneously, soybean biological seed coating agent can reduce the occurring degree of root rot, and the preventive effect of root rot is reached 73.5%.
The compound method that the used every production of biological seed coating agent of above-mentioned experiment is 1 ton is as follows:
(1) complex micro organism fungicide 200Kg: wherein (strain number is As1.828 to rhizobium solution, GIM1.222) (strain number is As1.217 for 20Kg, phosphate solubilizing bacteria solution, As1.223) (strain number is As1.190 for 40Kg, potassium solubilizing bacteria solution, As1.1870) (strain number is As1.941 for 40Kg, biocontrol microorganisms solution, As3.4034) 40Kg, degradation bacteria solution (strain number is As1.645) 20Kg, short living bacterium solution (strain number is As1.220, As1.866) 40Kg.The bacterial strain of above As numbering is all available from Institute of Micro-biology of the Chinese Academy of Sciences, and the bacterial strain of GIM numbering is available from Guangdong Institute of Micro-biology.The quantity of each effective bacterium is 10 in the above-mentioned complex micro organism fungicide 8Individual/more than the mg.
(2) biological regulator SOD M(patent No. 200510132464) 50Kg.
(3) trace element solution 100Kg, compound method: add boric acid (H in the 99.6Kg water 3BO 3) and ammonium molybdate [(NH 4) 2Mo 2O 7.4H 2O] each 0.2Kg.
(4) auxiliary agent 100Kg.Compound method: water 77.95Kg, urea 10Kg, polyvinyl alcohol 1788 (Industrial Co., Ltd. of the upper sea mail Qin provides) 10Kg, gibberellin GA 30.05Kg, sodium carboxymethylcellulose 1Kg, peregal 0-25 1Kg.
(5) the Banlangen addition is 4Kg.
(6) after above-mentioned each component mixes, finally add water to 1 ton.
Wherein the prescription of various medium is as follows:
Phosphate solubilizing bacteria, short living bacterium, degradation bacteria culture medium prescription: beef extract 20g, peptone 5g, NaCl1g add water to 1L, pH 7.0-7.2.
Potassium solubilizing bacteria culture medium prescription: glucose 5g, Na 2HPO 41g, MgSO 47H 2O 0.5g, FeCl 30.005g, CaCO 30.1g, add water to 1L, pH 7.0-7.2.
Rhizobium culture medium prescription: KH 2PO 40.2g, K 2HPO 40.8g, MgSO 47H 2O0.2g, CaSO 42H 2O 0.1g, FeCl 30.05g, Na 2MoO 42H 2O 0.05g, yeast extract 0.5g, mannitol 20g add water to 1L, pH 7.0-7.2.

Claims (1)

1. soybean biological seed coating agent, it is characterized in that: the agent of this kind clothing comprises following component, and each component is prepared by mass percentage: complex micro organism fungicide accounts for 20%, biological regulator SOD MAccount for 5%, trace element solution accounts for 10%, and auxiliary agent accounts for 10%, and Banlangen accounts for 0.4%, and surplus is water; Complex micro organism fungicide is comprised of rhizobium solution 10%, phosphate solubilizing bacteria solution 20%, potassium solubilizing bacteria solution 20%, biocontrol microorganisms solution 20%, degradation bacteria solution 10%, short living bacterium solution 20%, each component is prepared by mass percentage, and the quantity of each effective bacterium is 10 in the above-mentioned complex micro organism fungicide 8Individual/more than the mg; Trace element solution is comprised of water, boric acid and ammonium molybdate, and water, boric acid and ammonium molybdate shared mass percent in trace element is followed successively by 99.6%, 0.2%, 0.2%; Auxiliary agent is by water, urea, polyvinyl alcohol 1788, gibberellin GA 3, sodium carboxymethylcellulose and peregal 0-25 form water, urea, polyvinyl alcohol 1788, gibberellin GA 3, sodium carboxymethylcellulose and peregal 0-25 shared mass percent in auxiliary agent be respectively: 77.95%, 10%, 10%, 0.05%, 1%, 1%; Biological regulator SOD MBe a kind of superoxide dismutase mimics, have following chemical formula ,-[C 8H 8O 6N 2MXH 2O] n-, wherein M is any one of Mn, Fe, Ni, Zn, Mg, X is that 0~2, n is 5~7.
CN2009100731294A 2009-10-31 2009-10-31 Soybean biological seed coating agent Expired - Fee Related CN101723763B (en)

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Families Citing this family (8)

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Publication number Priority date Publication date Assignee Title
CN102308853A (en) * 2011-03-02 2012-01-11 沈阳农业大学 The biological seed coating agent of control soybean SCN
CN103539578A (en) * 2013-10-09 2014-01-29 中国石油天然气集团公司 Environment-friendly multifunctional composite soybean seed coating agent and processing technique thereof
CN103992190B (en) * 2014-05-23 2016-02-24 河南省农业科学院芝麻研究中心 A kind of sesame seed inorganic agent
CN105565979B (en) * 2015-12-22 2018-12-21 水利部牧区水利科学研究所 Leguminous seeds pill pelletizing coating
CN108283112A (en) * 2017-08-10 2018-07-17 黑龙江九穗谷农业科技发展有限公司 A kind of seed bud can be from the mulching film planting method of bursting film
CN107473831A (en) * 2017-08-31 2017-12-15 广西沙田仙人滩农业投资有限公司 A kind of seed dressing for improving Radix Codonopsis germination rate
CN111333454A (en) * 2020-04-01 2020-06-26 黑龙江八一农垦大学 Disease-preventing growth-promoting biological small red bean seed coating agent and preparation method and use method thereof
CN111393227B (en) * 2020-04-08 2022-07-08 黑龙江八一农垦大学 Seed coating agent

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1799361A (en) * 2006-01-13 2006-07-12 长沙普绿通生物科技有限公司 Biological seed coating agent
CN1806547A (en) * 2006-02-27 2006-07-26 黑龙江八一农垦大学 Biochemical long-acting soybean seed-coating agent
CN100347180C (en) * 2005-12-26 2007-11-07 北京华美天意科技开发有限公司 SOD simulated compound with short peptide as ligand and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100347180C (en) * 2005-12-26 2007-11-07 北京华美天意科技开发有限公司 SOD simulated compound with short peptide as ligand and preparation method thereof
CN1799361A (en) * 2006-01-13 2006-07-12 长沙普绿通生物科技有限公司 Biological seed coating agent
CN1806547A (en) * 2006-02-27 2006-07-26 黑龙江八一农垦大学 Biochemical long-acting soybean seed-coating agent

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
方淑梅等.大豆种衣剂田间筛选试验初报.《江西农业学报》.2008,第20卷(第1期),11-13. *

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