CN108496995A - A kind of biological seed dressing liquor and its preparation method and application - Google Patents
A kind of biological seed dressing liquor and its preparation method and application Download PDFInfo
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- CN108496995A CN108496995A CN201810275533.9A CN201810275533A CN108496995A CN 108496995 A CN108496995 A CN 108496995A CN 201810275533 A CN201810275533 A CN 201810275533A CN 108496995 A CN108496995 A CN 108496995A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to a kind of seed seed dressings comprising streptomyces albus, and are related to the preparation method and application of the seed dressing, belong to biologic product technology field.The biological seed dressing liquor includes the raw material composition of following parts by weight：50 80 parts of streptomyces albus microbial inoculum, 14 parts of potassium fulvate, 0.1 4 parts of Alkaloids From Sophora Alopecuroides L, 15 parts of sticker, 15 parts of wetting agent, 15 parts of dispersant, 0.1 4 parts of antifoaming agent；The streptomyces albus (Streptomyces albus), deposit number are CGMCC No.15117, and viable count is >=8 × 1010cfu/g.The seed dressing can be used for promoting plant growth, promote seed to sprout, enhance plant low-temperature resistance or promote seed sprouting, plant growth and enhancing disease resistance of plant simultaneously.
Description
Technical field
The present invention relates to a kind of biological seed dressing liquors, and in particular to a kind of seed seed dressing comprising streptomyces albus, and relate to
And the preparation method and application of the seed dressing, belong to biologic product technology field.
Background technology
" putting prevention first and combining prevention with control " are the plant protection policies that China always adheres to.Using seed dressing, seedling stage disease can be prevented
Evil and systemic disease, improve percentage of seedgermination, promote seedling healthy growth, be finally reached controlling disease, keep a full stand of seedings strong seedling, raising
The effect of crop quality and yield.Seed dressing economy is convenient, and utilization ratio is high, has guarantee in terms of seedling of protecting that seedlings are uniform and strong.
Reduce the use of seedling stage chemical pesticide.
Rely primarily on chemical seed dressing in production at present and prevented, however, the germ drug resistance of chemical agent, toxicity and
Problem of environmental pollution becomes increasingly conspicuous, and becomes the major obstacle for restricting agricultural sustainable development.Biological control has environment compatibility
Good, nontoxic advantage, and will not develop immunity to drugs, it is the ideal substitute of chemical agent.
Domestic seed treatment is mostly based on chemical composition at present, and biotic component is fewer, such as publication number
The invention of 105851087A《A kind of biological seed dressing liquor and preparation method thereof of prevention root rotof flax》It is small to disclose a kind of prevention
The biological seed dressing liquor of wheat root rot, each component are in parts by weight：16~24 parts of Acetamiprid, 3~11 parts of Ricinate, plant
9~17 parts of oil, 7~15 parts of bleeding agent, 2~4 parts of thickener, 3~5 parts of antifreezing agent, 1~3 part of antifoaming agent, aloe extract 1~3
Part, 0.5~1.5 part of Zhongshengmycin, 1~3 part of Alkaloids From Sophora Alopecuroides L, 1~3 part of potassium nitrate.The present invention also provides above-mentioned biologies to mix
The preparation method of kind agent.Whereby, the present invention is splendid to the control effect of root rotof flax；The wind loading rating of wheat enhances, anti-fall
Rate significantly improves, while wheat per mu yield also greatly improves；The content of protein, dietary fiber and vitamin E is apparent in wheat
It improves.
Publication number:The invention of 105875658A《A kind of cotton special bio seed dressing and preparation method thereof》Disclose one kind
Cotton special bio seed dressing, each component are in parts by weight：7~15 parts of 11~19 parts of propargite, solvent and cosolvent, emulsification
6~14 parts of agent, 3~5 parts of stabilizer, 2~4 parts of synergist, 13~21 parts of water, 2~6 parts of purple bergenia herb extract, octicin solution 1~3
Part, 1~3 part of vinblastine, 1~3 part of humic acid.The present invention also provides the preparation methods of above-mentioned biological seed dressing liquor.Whereby, this hair
The bright Common Diseases to cotton plants are effectively prevented；The disease resistance of cotton is not only increased, while to cotton planting
In common pest controling effect it is good；The present invention is safe, to the effect of being significantly improved of the yield of cotton, and in cotton
Long fibre ratio obviously increases.
Reality is in seed stage, and the germ for endangering seed is all weak parasite, and can solve this with biological pesticide asks
Topic.From safety, using chemical seed dressing, when improper use, can influence percentage of seedgermination, also be easy to cause environment dirt
Dye and pesticide residue, while being also unfavorable for the improvement of soil microorganism diversity.Therefore, exploitation new bio seed dressing is at seed
The direction of reason technology development.
Publication number:The invention of 105340974A《A kind of biological seed dressing liquor and application》Disclosed biological seed dressing liquor includes mostly viscous
Bacillus, amino-oligosaccharide and biochemical fulvic acid potassium, after seed dressing provided by the invention is coated crop seed so that
Seed surrounding soil forms an environment for being conducive to Seed Germination and growth, plays growth-promoting, strong sprout and disease-resistant effect.Three kinds
Main component compatibility has synergistic effect, is more advantageous to and promotes seed sprouting and plant strain growth.
Publication number:The invention of 104263677A《A kind of complex microorganism seed dressing and its preparation method and application》It discloses
A kind of complex microorganism seed dressing and its preparation method and application, complex microorganism seed dressing is by Sinorhizobium fredii, condensation
Bacillus, bacillus licheniformis, bacillus pumilus, bacillus amyloliquefaciens and turf compound.With fixed nitrogen, molten
Phosphorus, potassium decomposing, decomposition of cellulose and starch, generate auxin, it is disease-resistant the effects that；Also has the function of resistance, each other
Between compatibility it is good, the features such as condition of culture is easy to control；The complex microorganism seed dressing can be mixed thoroughly together with seed, be broadcast
Kind, composite fertilizer uses as base manure, to reduce the use of composite fertilizer.
Publication number:The invention of 102972447A《Biological seed dressing liquor》It includes soil beneficial microorganism vegetables to disclose a kind of
Seed seed dressing, the method for being related to preparing the biological seed dressing liquor belong to the biologic product technology field of vegetable seeds, main special
Point is withered comprising the bentonite as mounting medium, the starch as sticker and hydroxymethyl cellulose and soil beneficial microbe
Careless bacillus, bacillus licheniformis and Lou Che Shi streptomycetes, the present invention also have in addition to having disease-resistant, province kind, strong sprout and other effects
Have non-environmental-pollution, it is safe to use, promote root growth and development, enhancing crop drought resistance ability and anti-adversity ability, promote volume increase etc. it is excellent
Point.
Publication number:101531549 invention《Flax microorganism seed dressing agent》It solves existing use and defends good fortune, anthrax good fortune U.S. etc.
Wide-spectrum bactericide is dressed seed, and destroys heavier, the problem of causing soil secondary pollution to the beneficial microbe in soil, while also solving
Because long-term largely apply chemical fertilizer, microelement deficiencies is caused, flax low output and inferior problem are caused.The present invention presses
The multi-functional growth promoting bacteria agent of weight percent is 4~40% (number of viable is every milliliter 2~2,000,000,000 in seed dressing), by weight percentage
Than trace element be 0.02~3.0%, amino acid active substance by weight percentage is 2~10%, thickens by weight percentage
Agent is 0.05~3.0% and surplus is solvent synthesis by weight percentage.The present invention improves the diseases prevention ability of flax, flax seedling
Phase disease reduces by 90% or more, and survival rate of seedlings reaches 85%, and flax straw, seed production increase 10%.With it is efficient, green,
The advantage of environmental protection.
Existing biological seed dressing liquor be currently with promote the growth of plant, resist disease aspect based on, to seed resist
The ability of low temperature ability, acid and alkali-resistance environment does not refer to.Seed is very high to the temperature requirement of environment in early growth period, but
The often temperature instability in spring, it is fluctuated, the germination and growth of seed will be had an impact.
Invention content
The object of the present invention is to provide a kind of biological seed dressing liquor, main component be streptomyces albus microbial inoculum, potassium fulvate,
Alkaloids From Sophora Alopecuroides L, sticker, wetting agent, dispersant, antifoaming agent.We provide it is a kind of safer to seed, to environment more ring
The biological seed dressing liquor of guarantor.The temperature range for the streptomyces albus microbial inoculum growth that we are added simultaneously is wider, in 12-40 DEG C of model
Enclosing interior can grow.It can improve the rhizospheric environment of seed after use, increase the anti-seismic design of seed, prevent because of gas
The germination that is unstable and influencing seed of temperature.This white streptomycete bacterial strain also has stronger acidproof alkali ability, can be by pH value
Ranging from 3-12.This streptomyces albus it is sourer and in the environment of compared with alkali can normal growth, can be in acid soil and alkaline soil
It survives in earth and generates a series of factors for being beneficial to seed growth, and our biological seed dressing liquor is made by the collaboration of each ingredient
With can have the function that preferably to prevent sprout term disease and promote to grow.
A kind of biological seed dressing liquor includes the raw material composition of following parts by weight：
50-80 parts of streptomyces albus microbial inoculum, 1-4 parts of potassium fulvate, 0.1-4 parts of Alkaloids From Sophora Alopecuroides L, 1-5 parts of sticker,
1-5 parts of wetting agent, 1-5 parts of dispersant, 0.1-4 parts of antifoaming agent；
The viable count of the streptomyces albus microbial inoculum is >=8 × 1010cfu/g；
Preferably, the sticker is xanthans or polyvinyl alcohol；
Preferably, the wetting agent is pull open powder or lauryl sodium sulfate；
Preferably, the dispersant is NNO (2- naphthalene sulfonic acid formaldehyde polymers sodium salt) or sodium lignin sulfonate；
Preferably, the antifoaming agent is organic silicon defoamer；
Preferably, the biological seed dressing liquor includes the raw material composition of following parts by weight：
55-75 parts of streptomyces albus microbial inoculum, 1-3 parts of potassium fulvate, 0.2-3.5 parts of Alkaloids From Sophora Alopecuroides L, sticker 1-4
Part, 1-4 parts of wetting agent, 1-4 parts of dispersant, 0.1-3 parts of antifoaming agent；
Preferably, the biological seed dressing liquor includes the raw material composition of following parts by weight：
60-70 parts of streptomyces albus microbial inoculum, 2-3 parts of potassium fulvate, 0.5-3 parts of Alkaloids From Sophora Alopecuroides L, 2-4 parts of sticker,
2-4 parts of wetting agent, 2-4 parts of dispersant, 0.1-2 parts of antifoaming agent；
Preferably, the biological seed dressing liquor includes the raw material composition of following parts by weight：
65-68 parts of streptomyces albus microbial inoculum, 2-3 parts of potassium fulvate, 0.6-2.5 parts of Alkaloids From Sophora Alopecuroides L, sticker 2-3
Part, 2-3 parts of wetting agent, 2-3 parts of dispersant, 0.5-1 parts of antifoaming agent；
Preferably, the biological seed dressing liquor includes the raw material composition of following parts by weight：
65 parts of streptomyces albus microbial inoculum, 2.5 parts of potassium fulvate, 2.5 parts of Alkaloids From Sophora Alopecuroides L, 2.8 parts of sticker, wetting agent
3 parts, 3 parts of dispersant, 1.2 parts of antifoaming agent.
The viable count of the streptomyces albus microbial inoculum is >=8 × 1010cfu/g；
The sticker is xanthans；
The wetting agent is lauryl sodium sulfate；
The dispersant is NNO；
The antifoaming agent is organic silicon defoamer；
The preparation method of the biological seed dressing liquor, includes the following steps：Potassium fulvate and Alkaloids From Sophora Alopecuroides L are put into mixed
It mixes after puddling 2min in machine, streptomyces albus microbial inoculum is put into grinder and is ground 1min, then the material mixing is arrived
Together, it is uniformly mixed.
The preparation method of the streptomyces albus microbial inoculum, it is specific as follows：
It is prepared by spore suspension：Sterile water is added to the inclined-plane for the Gause I solid medium for preserving streptomyces albus
On, and then spore is scraped, mixing is shaken, obtains spore suspension, the cell concentration of the spore suspension is 108-109cfu/mL；
Seed liquor culture：The spore suspension is inoculated by inoculum concentration 1-5% (v/v) in seed liquid culture medium, 28 DEG C,
150-220r/min, shaking flask culture 85-95h, obtains seed liquor；
Solid fermentation culture：The seed liquor is inoculated into water content by inoculum concentration volume mass ratio 5-15% (v/m)
In 50% solid fermentation culture medium, 28 DEG C of koji plate fermentation cultures 4-7 days.20-30 DEG C air-dries, and is ground with pulverizer, collects logical
The microbial inoculum for crossing 120 mesh sieve stores in room temperature shady place, obtains streptomyces albus microbial inoculum, the bacterium of the streptomyces albus microbial inoculum
Kind vigor is not less than 8 × 1010cfu/g；
The Gause I solid medium composition is as follows：Soluble starch 20g/L, K2HPO40.5g/L, KNO3 1g/
L, MgSO4 .7H2O 0.5g/L, FeSO4 .7H2O 0.01g/L, agar 20g/L, surplus are distilled water, pH 7.2-7.4；
The seed liquid culture medium composition is as follows：Corn flour 10g/L, peptone 10g/L, yeast powder 5g/L, phosphoric acid hydrogen two
Potassium 2g/L, magnesium sulfate 0.3g/L, surplus are distilled water, and pH is natural.
The solid fermentation culture medium composition is as follows：Rice husk 18g/L, wheat bran 45.7g/L, corn flour 23g/L, beancake powder
12g/L, calcium carbonate 0.8g/L, dipotassium hydrogen phosphate 0.3g/L, magnesium sulfate 0.2g/L, surplus are distilled water, and pH is natural.
A kind of application of biological seed dressing liquor is included in the application promoted in plant growth；Application in promoting seed to sprout；
Enhance the application in plant low-temperature resistance；Answering including promoting seed to sprout at the same time, in plant growth and enhancing disease resistance of plant
With.Including being stirred evenly after mixing seed dressing with seed, spreads out and dry.
The application of the biological seed dressing liquor, it is proposed that：Dress seed peanut, corn and soybean pesticide-seeds ratio be 1:300, seed dressing tomato,
The pesticide-seeds ratio of cucumber, capsicum etc. is 1:150.In use, according to the dosage of seed, the seed dressing of corresponding weight is weighed, will be dressed seed
A small amount of water is added in agent, seed dressing can be dissolved and just mix to open by the seed to be dressed seed by the water of addition to be mixed thoroughly.Kind
Shady place is spread out after son seed dressing to dry in the shade.It can be sowed.
The streptomyces albus microbial inoculum bacterial strain is streptomyces albus (Streptomyces albus), and deposit number is
CGMCC No.15117。
The streptomyces albus is isolated from the cherry Orchard Soil of the Hebei province Shanhai Pass.White provided by the present invention
Streptomycete (Streptomyces albus) LM has been preserved in Chinese microorganism strain preservation management committee on December 25th, 2017
Member's meeting common micro-organisms center (CGMCC), deposit number are CGMCC No.15117, and address is BeiChen West Road, Chaoyang District, BeiJing City
No. 1 institute 3, Institute of Microorganism, Academia Sinica.
Physio-biochemical characteristics：
With reference to primary Jie Shi identification handbooks, following Physiology and biochemistry identification is carried out to bacterial strain RJ-36b：Streptomycin resistance is tested, bright
Dispergation, milk peptonize, Starch Hydrolysis, nitrate reduction, and hydrogen sulfide experiment to the tolerance test of salt, the results are shown in Table 1.
Table 1：Physiology and biochemistry qualification result
| Experiment | As a result |
| Streptomycin resistance | — |
| Gelatin liquefaction | + |
| Milk peptonizes | + |
| Starch Hydrolysis | + |
| Nitrate reduction | + |
| Hydrogen sulfide is tested | — |
| NaCl resistant proofs | >=10%<13% |
Note：1, positive+, 2, negative-
Utilization of carbon source
Strain is inoculated in respectively containing D-Glucose, starch, sucrose, D- xyloses, D-Fructose, L-arabinose, D- galas
Sugar, L- rhamnoses, PEARLITOL 25C on the different carbon source tablet of inositol, after 32 DEG C of culture 7d, observe strain growth situation, as a result
It is shown in Table 2.
By following as a result, illustrate that the utilization scope of the bacterium carbon source is wide, a variety of sugar can be utilized.
Table 2：Utilization of carbon source result
| Carbon source | As a result |
| D-Glucose | + |
| Sucrose | + |
| D-Fructose | + |
| D- galactolipins | + |
| PEARLITOL 25C | + |
| Starch | + |
| D- xyloses | + |
| L-arabinose | — |
| L- rhamnoses | — |
| Inositol | — |
Note：1, using+, 2, do not utilize-
Streptomyces albus (Streptomyces albus) LM can be grown within the scope of 12-40 DEG C, the most suitable growth temperature
Degree is 28 DEG C；A variety of degrading enzymes such as protease, lipase can be generated.
Gao Shis mono- of streptomyces albus (Streptomyces albus) LM in the different pH values that pH value is 3-12
It is grown in number fluid nutrient medium, after 50 DEG C of culture 3d on Gause I tablet in coating and Gause I fluid nutrient medium test tube
50 DEG C of culture 3d, energy well-grown, the results are shown in Table 3.Two experiments prove that the bacterial strain can tolerate the ranging from 3-12 of pH value,
It is more wide in range, there is stronger soda acid tolerance.
Table 3：Colony characteristics
| Culture medium | Situation | Aerial hyphae | Substrate mycelium | It can lysochrome |
| Gause I agar medium | It is good | White | Yellow | Nothing |
| Oat-agar cultures base | It is good | White | It is yellowish-brown | Nothing |
| Brewer's wort yeast extract agar culture medium | It is good | White | White | Nothing |
| Asparagine agar medium | Difference | White | White | Nothing |
Advantageous effect
Streptomyces albus bacterial strain used in biological seed dressing liquor of the present invention has wider growth temperature range, at 12-40 DEG C
It can grow, it can improve the environment around seed rhizosphere when to low temperature environment, increase the anti-seismic design of seed.
This white streptomycete bacterial strain also has stronger acidproof alkali ability, can be by the ranging from 3-12 of pH value.This streptomyces albus compared with
Acid and compared in the environment of alkali can normal growth, can survive and generate in acid soil and basic soil a series of is beneficial to plant
The substance of son growth.Our biological seed dressing liquor is also added into potassium fulvate and Sophora alopecuroide in addition to having streptomyces albus microbial inoculum
Alkaloid, three kinds of ingredients can preferably promote plant strain growth and resist the disease in seedling stage by synergistic effect.
Streptomyces albus provided by the invention not only has the function of preventing some common soil-borne diseases also have outside resisting
The interference of boundary's low temperature, to not influence the germination of seed.For domestic seed dressing mostly based on chemical composition, improper use can be to seed
Germination and growth have a certain impact, and we select bacteria agent not only have the function of good biological and ecological methods to prevent plant disease, pests, and erosion, to seed tool
There is absolute safety.
Formula provided by the invention has synergistic effect compared with single composition.The present invention with streptomyces albus, potassium fulvate,
Alkaloids From Sophora Alopecuroides L is main seed dressing ingredient, these three ingredients are the biomaterial of safety and environmental protection, and a combination thereof can cooperate with
Seedling stage crop disease is prevented, promotes plant growth, influence of the extraneous low temperature to seed can be resisted.And this three kinds combination currently without
Report as seed dressing.
Biological seed dressing liquor of the present invention is greatly improved the emergence rate of plant, plant height, root long, and fresh weight etc. goes out by taking tomato as an example
Seedling rate is higher by 6.1% compared with blank control, and plant height is higher by 4.76cm compared with blank control, and fresh weight is higher by 1.19g, root long compared with blank control
It is higher by 1.09cm compared with blank.
Biological seed dressing liquor ingredient of the present invention is simple, and main component is probiotics and raw material prepared by plant, preparation method
Simply, special processing is not needed, of low cost, biocontrol effect protrudes.What is more important is nontoxic, pollution-free, production, transport and
Storage and easy to use, particularly suitable organic plantation, with fabulous application prospect.
Specific implementation mode
Embodiment 1：
65 parts of streptomyces albus microbial inoculum, 2.5 parts of potassium fulvate, 2.5 parts of Alkaloids From Sophora Alopecuroides L, 2.8 parts of sticker, wetting agent
3 parts, 3 parts of dispersant, 1.2 parts of antifoaming agent；
The preparation method of the biological seed dressing liquor, includes the following steps：Potassium fulvate and Alkaloids From Sophora Alopecuroides L are put into mixed
It mixes after puddling 2min in machine, streptomyces albus microbial inoculum is put into grinder and is ground 1min, then the material mixing is arrived
Together, it is uniformly mixed.
The preparation method of the streptomyces albus microbial inoculum, it is specific as follows：
It is prepared by spore suspension：Sterile water is added to the inclined-plane for the Gause I solid medium for preserving streptomyces albus
On, and then spore is scraped, mixing is shaken, obtains spore suspension, the cell concentration of the spore suspension is 108-109cfu/mL。
Seed liquor culture：The spore suspension is inoculated by inoculum concentration 1-5% (v/v) in seed liquid culture medium, 28 DEG C,
150-220r/min, shaking flask culture 85-95h, obtains seed liquor；
Solid fermentation culture：The seed liquor is inoculated into water content by inoculum concentration volume mass ratio 5-15% (v/m)
In 50% solid fermentation culture medium, 28 DEG C of koji plate fermentation cultures 4-7 days.20-30 DEG C air-dries, and is ground with pulverizer, collects logical
The microbial inoculum for crossing 120 mesh sieve stores in room temperature shady place, obtains streptomyces albus microbial inoculum, the bacterium of the streptomyces albus microbial inoculum
Kind vigor is not less than 8 × 1010cfu/g。
Embodiment 2：
80 parts of streptomyces albus microbial inoculum, 4 parts of potassium fulvate, 4 parts of Alkaloids From Sophora Alopecuroides L, 5 parts of sticker, wetting agent 5 part, point
5 parts of powder, 4 parts of antifoaming agent；
The preparation method is the same as that of Example 1 for the biological seed dressing liquor.
Embodiment 3：
50 parts of streptomyces albus microbial inoculum, 1 part of potassium fulvate, 0.2 part of Alkaloids From Sophora Alopecuroides L, 1 part of sticker, wetting agent 1.5
Part, 1 part of dispersant, 0.2 part of antifoaming agent；
The preparation method is the same as that of Example 1 for the biological seed dressing liquor.
Embodiment 4：
70 parts of streptomyces albus microbial inoculum, 3 parts of potassium fulvate, 3 parts of Alkaloids From Sophora Alopecuroides L, 3.5 parts of sticker, 4 parts of wetting agent,
4 parts of dispersant, 2 parts of antifoaming agent；
The preparation method is the same as that of Example 1 for the biological seed dressing liquor.
Embodiment 5：
60 parts of streptomyces albus microbial inoculum, 2 parts of potassium fulvate, 2 parts of Alkaloids From Sophora Alopecuroides L, 2 parts of sticker, wetting agent 2 part, point
2 parts of powder, 1.5 parts of antifoaming agent；
The preparation method is the same as that of Example 1 for the biological seed dressing liquor.
Experimental example 1：
Based on the formula of embodiment 1,65 parts of streptomyces albus microbial inoculum, 2.5 parts of potassium fulvate, Alkaloids From Sophora Alopecuroides L
2.5 parts, 2.8 parts of sticker, 3 parts of wetting agent, 3 parts of dispersant, 1.2 parts of antifoaming agent.
The seed of streptomyces albus microbial inoculum, Alkaloids From Sophora Alopecuroides L, potassium fulvate and biological seed dressing liquor to tomato is used respectively
It dresses seed, is control with market biological seed dressing liquor on sale, each processing 100 is repeated 3 times.After 30 days to its germination percentage,
Plant height, root long, fresh weight measure, and the results are shown in Table 4.
Table 4:Biological seed dressing liquor of the present invention is with each one-component to tomato seed dressing control experiment result
| Processing | Germination percentage (%) | Plant height (cm) | Fresh weight (g) | Root long (cm) |
| Streptomyces albus microbial inoculum | 86.3c | 20.35b | 2.12b | 9.13b |
| Alkaloids From Sophora Alopecuroides L | 83.6c | 19.34b | 2.05b | 9.01b |
| Potassium fulvate | 89.2b | 21.55b | 2.38b | 9.35b |
| Biological seed dressing liquor | 94.5a | 24.88a | 3.15a | 10.12a |
The processing germination percentage of biological seed dressing liquor of the present invention, plant height, fresh weight, root long are can be seen that by table above all
Significantly better than the processing of streptomyces albus microbial inoculum, Sophora alopecuroides alkali process, the biology seed dressing that potassium fulvate is handled and market is on sale
Agent is handled, and having differences property is notable.It can be seen that biological seed dressing liquor of the present invention has synergistic effect, it is better than exclusive use
Effect.
Experimental example 2：
Damping-off pathogen is activated on PDA, after growing 7 days, the mycelia of damping-off pathogen is scraped sterile
In the triangular flask of water, and shaken up.Uniformly it is sprinkled into nursery soil.
Based on the formula of embodiment 1,65 parts of streptomyces albus microbial inoculum, 2.5 parts of potassium fulvate, Alkaloids From Sophora Alopecuroides L
2.5 parts, 2.8 parts of sticker, 3 parts of wetting agent, 3 parts of dispersant, 1.2 parts of antifoaming agent.
Use respectively streptomyces albus microbial inoculum, Alkaloids From Sophora Alopecuroides L and the mixed biological agent of the two to the seed of tomato into
Row seed dressing, to be not added with any substance as blank control.Each processing 100, is repeated 3 times.It unites to its incidence after 20 days
Meter, the results are shown in Table 5.
Table 5：Streptomyces albus microbial inoculum, Alkaloids From Sophora Alopecuroides L are compound and each one-component is to tomato seed dressing control experiment result
Can significantly it be seen by data above, streptomyces albus microbial inoculum and the mixed processing of Alkaloids From Sophora Alopecuroides L
Incidence is significantly lower than the two individually processing, hence it is evident that is less than blank control.Corresponding preventive effect is also that the processing of the two mixing is bright
It is aobvious individually to be handled higher than the two, it is higher than blank control.Explanation：Both streptomyces albus microbial inoculum, Alkaloids From Sophora Alopecuroides L mixing tool
There is synergistic effect, is better than the effect of exclusive use.
Experimental example 3：
Choose the formula of embodiment 1, streptomyces albus microbial inoculum:65 parts, 2.5 parts of potassium fulvate, Alkaloids From Sophora Alopecuroides L 2.5
Part, 2.8 parts of sticker, 3 parts of wetting agent, 3 parts of dispersant, 1.2 parts of antifoaming agent.
100g seed dressings are weighed, by seed dressing and water with 1:4 uniformly mixing, and by seed dressing and seed with mass ratio 1:
150 dress seed, and seed is tomato, and seed is spread out drying after seed dressing, spare after drying.
Experiment is divided to two processing, processing 1 to be the tomato seeds after seed dressing of the present invention seed dressing, and processing 2 is market purchase
Tomato seeds after seed dressing seed dressing.Each 100 seeds of processing, are repeated 3 times.
After emergence 7 days, while two processing being put into illumination box, and low temperature environment is set.22 DEG C of daytime, night
12 DEG C of evening places 48h.By two processing, taking-up allows its continued growth indoors simultaneously after 48h, 25 DEG C of daytime, 16 DEG C of night.It waits for
The planting percent that tomato is checked when growing to 2 true leaves, the results are shown in Table 6.
Planting percent of 6 tomato of table after low-temperature treatment when two panels true leaf
| Processing | Planting percent % |
| Processing 1 | 94.7a |
| Processing 2 | 78.6b |
After low-temperature treatment, its planting percent is checked in the two panels true leaf of tomato, after same low-temperature treatment, kind
For eggplant seed by the processing of biological seed dressing liquor of the present invention, survival rate is relatively high, can reach 94.7%, and the seed dressing that market is bought
Agent is handled, and planting percent 78.6%, the two has differed 16.1%.Bigger difference is shown, and having differences property is notable.
It can illustrate that the seed dressing of the present invention is had excellent performance at low temperature resistant aspect than seed dressing on sale.
Experimental example 4 chooses the formula of embodiment 1,65 parts of streptomyces albus microbial inoculum, 2.5 parts of potassium fulvate, Sophora alopecuroides
2.5 parts of alkali, 2.8 parts of sticker, 3 parts of wetting agent, 3 parts of dispersant, 1.2 parts of antifoaming agent.Corn seed is pressed into seed dressing and seed
Mass ratio 1:300 dress seed, and tomato seeds are by seed dressing with seed quality than 1:150 dress seed, and seed dressing is added a small amount of
Water, can seed dressing be dissolved and seed can just be mixed thoroughly be advisable.Two kinds of seeds are spread out drying, standby to be dried after seed dressing
With.
This experiment is carried out in May, 2017 in Shanhaiguan District proving ground, and corn trials are divided into two processing, by this hair
The processing of bright biological seed dressing liquor Dressing and the seed dressing Dressing bought on the market, tomato is also two processing, is passed through
Biological seed dressing liquor Dressing of the present invention and the seed dressing Dressing bought on the market, experiment are set as 3 repetitions.
The emergence rate of corn, plant height, fresh weight and root long are detected after 20 days, the results are shown in Table 7：
7 seed dressing of table influences the situation table of comparisons to corn
| Processing | Emergence rate | Plant height | Fresh weight | Root long |
| Biological seed dressing liquor of the present invention | 95.42a | 43.74a | 3.79a | 15.43a |
| Market seed dressing | 86.33b | 39.21b | 2.45b | 12.54b |
It can be seen that the emergence rate of the corn after biological seed dressing liquor of the present invention seed dressing by the data of table 2, plant height is fresh
Weight, root long is all significantly better than the processing of the seed dressing of market purchase, and having differences property is notable.Wherein emergence rate is dressed seed than market
Agent has been higher by 9.09%, and for plant height than the 4.53cm that has been higher by of market seed dressing, fresh weight improves 1.34g, and root long improves
2.89cm.It can be seen that biological seed dressing liquor of the present invention has very high advantage to corn seed dressing.With the seed dressing phase of market purchase
Than substantially increasing its emergence rate, plant height, fresh weight, root long etc..
The emergence rate of tomato, plant height, fresh weight and root long are detected after 20 days, are shown in Table 8：
8 seed dressing of table influences the situation table of comparisons to tomato
| Processing | Emergence rate | Plant height | Fresh weight | Root long |
| Biological seed dressing liquor of the present invention | 94.34a | 19.43a | 3.03a | 15.86a |
| Market seed dressing | 84.75b | 15.65b | 2.15b | 12.11b |
Tomato its emergence rate after biological seed dressing liquor of the present invention seed dressing is can be seen that by the data of upper table, plant height,
Fresh weight, root long is all significantly better than the processing of market seed dressing, and having differences property is notable, and wherein emergence rate is than market seed dressing
It has been higher by 9.59%, for plant height than the 3.78cm that has been higher by of market seed dressing, fresh weight improves 0.88g, and root long improves 3.75cm.
It can be seen that biological seed dressing liquor of the present invention has very high advantage to tomato seed dressing.It, can be big compared with the seed dressing of market purchase
Its emergence rate, plant height, root long, fresh weight etc. are improved greatly.It should be noted that biological seed dressing liquor prepared by 2-5 of the embodiment of the present invention is same
Sample has above-mentioned test effect, and otherness is not notable between each embodiment, and wherein 1 effect of embodiment is slightly good.
Claims (10)
1. a kind of biological seed dressing liquor includes the raw material composition of following parts by weight：
50-80 parts of streptomyces albus microbial inoculum, 1-4 parts of potassium fulvate, 0.1-4 parts of Alkaloids From Sophora Alopecuroides L, 1-5 parts of sticker, moistening
1-5 parts of agent, 1-5 parts of dispersant, 0.1-4 parts of antifoaming agent；
The viable count of the streptomyces albus microbial inoculum is >=8 × 1010cfu/g。
2. biological seed dressing liquor according to claim 1, which is characterized in that the streptomyces albus (Streptomyces
Albus), deposit number is CGMCC No.15117.
3. biological seed dressing liquor according to claim 2, which is characterized in that the raw material composition including following parts by weight：
It is 55-75 parts of streptomyces albus microbial inoculum, 1-3 parts of potassium fulvate, 0.2-3.5 parts of Alkaloids From Sophora Alopecuroides L, 1-4 parts of sticker, wet
Moisten 1-4 parts of agent, 1-4 parts of dispersant, 0.1-3 parts of antifoaming agent.
4. biological seed dressing liquor according to claim 2, which is characterized in that the raw material composition including following parts by weight：
60-70 parts of streptomyces albus microbial inoculum, 2-3 parts of potassium fulvate, 0.5-3 parts of Alkaloids From Sophora Alopecuroides L, 2-4 parts of sticker, moistening
2-4 parts of agent, 2-4 parts of dispersant, 0.1-2 parts of antifoaming agent.
5. biological seed dressing liquor according to claim 2, which is characterized in that the raw material composition including following parts by weight：
It is 65-68 parts of streptomyces albus microbial inoculum, 2-3 parts of potassium fulvate, 0.6-2.5 parts of Alkaloids From Sophora Alopecuroides L, 2-3 parts of sticker, wet
Moisten 2-3 parts of agent, 2-3 parts of dispersant, 0.5-1 parts of antifoaming agent.
6. biological seed dressing liquor according to claim 2, which is characterized in that the raw material composition including following parts by weight：
65 parts of streptomyces albus microbial inoculum, 2.5 parts of potassium fulvate, 2.5 parts of Alkaloids From Sophora Alopecuroides L, 2.8 parts of sticker, 3 parts of wetting agent,
3 parts of dispersant, 1.2 parts of antifoaming agent.
7. a kind of preparation method of biological seed dressing liquor, includes the following steps：Potassium fulvate and Alkaloids From Sophora Alopecuroides L are put into and puddled
After puddling 2min in machine, streptomyces albus microbial inoculum is put into grinder and is ground 1min, then by the material mixing to one
It rises, is uniformly mixed.
8. the preparation method of biological seed dressing liquor according to claim 7, which is characterized in that the system of the streptomyces albus microbial inoculum
Preparation Method includes the following steps：
It is prepared by spore suspension：Sterile water is added on the inclined-plane of Gause I solid medium for preserving streptomyces albus, into
And spore is scraped, mixing is shaken, spore suspension is obtained, the cell concentration of the spore suspension is 108-109cfu/mL；
Seed liquor culture：The spore suspension is inoculated by inoculum concentration 1-5% (v/v) in seed liquid culture medium, 28 DEG C, 150-
220r/min, shaking flask culture 85-95h, obtains seed liquor；
Solid fermentation culture：The seed liquor is inoculated into water content 50% by inoculum concentration volume mass ratio 5-15% (v/m)
In solid fermentation culture medium, 28 DEG C of koji plate fermentation cultures 4-7 days；20-30 DEG C air-dries, and is ground with pulverizer, collects and passes through 120 mesh
The microbial inoculum of sieve stores in room temperature shady place, obtains streptomyces albus microbial inoculum；
The Gause I solid medium composition is as follows：Soluble starch 20g/L, K2HPO40.5g/L, KNO31g/L,
MgSO4·7H2O 0.5g/L, FeSO4·7H2O 0.01g/L, agar 20g/L, surplus are distilled water, pH 7.2-7.4；
The seed liquid culture medium composition is as follows：Corn flour 10g/L, peptone 10g/L, yeast powder 5g/L, dipotassium hydrogen phosphate 2g/
L, magnesium sulfate 0.3g/L, surplus are distilled water, and pH is natural；
The solid fermentation culture medium composition is as follows：Rice husk 18g/L, wheat bran 45.7g/L, corn flour 23g/L, beancake powder 12g/L,
Calcium carbonate 0.8g/L, dipotassium hydrogen phosphate 0.3g/L, magnesium sulfate 0.2g/L, surplus are distilled water, and pH is natural.
9. any biological seed dressing liquors of claim 1-6 in promoting plant growth application, promote seed sprout in
Using, in enhancing plant low-temperature resistance application, at the same time promote seed sprout, plant growth and enhancing disease resistance of plant in
Using.
10. application according to claim 9, which is characterized in that seed dressing peanut, corn and soybean pharmacopoeia weight ratio be 1:
300, the pharmacopoeia weight ratio of seed dressing tomato, cucumber, capsicum etc. is 1:150.
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| CN111557308A (en) * | 2020-04-24 | 2020-08-21 | 山东省农业科学院生物技术研究中心 | Special microbial composition for peanuts as well as preparation method and application thereof |
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| CN105340974A (en) * | 2015-10-30 | 2016-02-24 | 湖北省生物农药工程研究中心 | Biological seed dressing agent and application thereof |
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| CN105340974A (en) * | 2015-10-30 | 2016-02-24 | 湖北省生物农药工程研究中心 | Biological seed dressing agent and application thereof |
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| CN109136142B (en) * | 2018-09-10 | 2021-10-08 | 天津科技大学 | Streptomyces alboflavus, method for preparing biocontrol microbial inoculum by using same and application thereof |
| CN111557308A (en) * | 2020-04-24 | 2020-08-21 | 山东省农业科学院生物技术研究中心 | Special microbial composition for peanuts as well as preparation method and application thereof |
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