CN101688203B - 突变的g蛋白偶联受体及其选择方法 - Google Patents

突变的g蛋白偶联受体及其选择方法 Download PDF

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CN101688203B
CN101688203B CN2008800093780A CN200880009378A CN101688203B CN 101688203 B CN101688203 B CN 101688203B CN 2008800093780 A CN2008800093780 A CN 2008800093780A CN 200880009378 A CN200880009378 A CN 200880009378A CN 101688203 B CN101688203 B CN 101688203B
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gpcr
ligand
mutant
receptor
amino acid
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CN101688203A (zh
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R·亨德松
C·G·塔特
F·马尼亚尼
M·J·塞拉诺-维加
Y·芝多
A·J·瓦内
M·P·韦尔
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Nexla Pharmaceuticals Uk Ltd
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Heptares Therapeutics Ltd
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Priority claimed from GB0724052A external-priority patent/GB0724052D0/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

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  • Biomedical Technology (AREA)
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  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN2008800093780A 2007-03-22 2008-03-20 突变的g蛋白偶联受体及其选择方法 Active CN101688203B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0705450A GB0705450D0 (en) 2007-03-22 2007-03-22 Mutant proteins and method for selecting them
GB0705450.5 2007-03-22
GB0724052A GB0724052D0 (en) 2007-12-08 2007-12-08 Mutant proteins and methods for selecting them
GB0724052.6 2007-12-08
PCT/GB2008/000986 WO2008114020A2 (en) 2007-03-22 2008-03-20 Mutant g-protein coupled receptors and methods for selecting them

Related Child Applications (1)

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CN201310556727.3A Division CN103641908A (zh) 2007-03-22 2008-03-20 突变的g蛋白偶联受体及其选择方法

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CN101688203A CN101688203A (zh) 2010-03-31
CN101688203B true CN101688203B (zh) 2013-12-11

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CN201310556727.3A Pending CN103641908A (zh) 2007-03-22 2008-03-20 突变的g蛋白偶联受体及其选择方法

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US (7) US8785135B2 (https=)
EP (4) EP2386635A3 (https=)
JP (6) JP4842400B2 (https=)
CN (2) CN101688203B (https=)
AT (1) ATE544854T1 (https=)
AU (1) AU2008228085B2 (https=)
CA (1) CA2681415C (https=)
DK (1) DK2121919T3 (https=)
ES (2) ES3008359T3 (https=)
GB (4) GB2456236B8 (https=)
HR (1) HRP20120336T1 (https=)
PL (1) PL2121919T3 (https=)
PT (1) PT2121919E (https=)
WO (1) WO2008114020A2 (https=)

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WO2008068534A2 (en) * 2008-03-05 2008-06-12 Heptares Therapeutics Limited Crystal structure of a betal -adremergi c receptor and uses thereof
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WO2012120315A2 (en) 2011-03-10 2012-09-13 Heptares Therapeutics Limited Mutant proteins and methods for producing them
US10174101B2 (en) 2011-08-10 2019-01-08 Heptares Therapeutics Limited Stable proteins
ES2676827T3 (es) * 2012-06-01 2018-07-25 Heptares Therapeutics Limited Ensayos
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CN105593240B (zh) * 2013-06-11 2024-05-31 保罗·谢勒学院 用于以单氨基酸分辨率确定可突变配体-gpcr结合的方法以及突变配体和gpcr对
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CN114038498B (zh) * 2022-01-06 2022-03-18 北京晶泰科技有限公司 Gpcr的热稳定性突变预测方法、结构筛选方法及其装置
CN120948816B (zh) * 2025-10-20 2026-03-03 国科大杭州高等研究院 一种g蛋白偶联受体的热稳定性突变位点筛选方法及其用途

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