CN101664215B - Compound beverage containing probiotics and collagen and preparation method thereof - Google Patents
Compound beverage containing probiotics and collagen and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of foods and particularly relates to a compound beverage containing probiotics and collagen and a preparation method thereof. In order to solve the problem that the probiotics in the existing probiotics beverage is difficult to store for long period, the probiotics and the collagen are separately packaged in the invention and then combined to form the compound beverage containing probiotics and collagen. The compound beverage includes a solid part and a liquid part, wherein the solid part at least includes the probiotics, and the liquid part includes a fixed component and an unfixed component; the fixed component is the collagen; and the solid part also comprises an auxiliary carrier used for prolonging the survival time of probiotics. The technical proposal can prolong the survival time of probiotics by at least twice or more.
Description
Technical field
The invention belongs to food technology field, be specifically related to a kind of composite beverage that contains probio and collagen and preparation method thereof.
Background technology
The probio collagen oral liquid is a kind of composite beverage, and is main by additional human collagen albumen and probio, and makes human body more healthy.Collagen is by the synthetic a kind of boiomacromolecule of zooblast, is a kind of white, opaque, unbranched fibrous type protein, and it has the function that supports organ and protection human body, also is the most important functions albumen that forms cytoplasm.Collagen with its natural hypotoxicity, low antigenicity, hypoimmunity, can suppress Epithelial cell migration, can guide cytothesis and good with human compatibility.The fibre structure of collagen uniqueness and good biocompatibility thereof and low antigenicity make it become the cosmetics of super quality, the popular research topic of functional health-care food and field of biomedical materials.Can replenish the required nutrient of skin layer, replenish 17 kinds of amino acid to the human body beneficial, the integrality that keeps cuticula moisture and fibre structure, improve the metabolism that the Skin Cell living environment promotes skin histology, strengthen circulation, reach skin care, delay senility, beauty treatment, wrinkle-chasing, the purpose of hair care.
Traditional beverage or the preparation method of oral liquid are by the utility in the raw material is extracted, join again other raw materials, add an amount of pure water, be made into liquid or liquid and solids mixing material, can is in vial, in plastic bottle, ceramic bottle or the metal can, sealing, sterilization.Also there are the front material of can and packing container to sterilize respectively.The collagen beverage major part also is liquid, in the probiotic products of liquid the time-to-live of probio very short, through after a while preservation or transportation, active probiotic quantity sharply descends, often when the consumer drank probiotic beverage, the probio in the probiotic beverage was substantially dead.
Summary of the invention
Technical problem to be solved by this invention is that the probio in the existing probiotic beverage is difficult to the problem of long preservation, and a kind of composite beverage that contains probio and collagen and preparation method thereof is provided.
The present invention solves the problems of the technologies described above by following technical solution:
A kind of composite beverage that contains probio and collagen, comprise solid portion and liquid part, described solid portion comprises probio at least, described liquid partly comprises fixedly component and on-fixed component, described fixedly component is collagen, described solid portion comprises that also the probio that above-mentioned solid portion comprises at least can be the probiotics bacterial powder, also can be probiotic microcapsule particle etc. for the assistant carrier that prolongs the probio survival period.
The present invention uses the problem that two kinds of probios in the means solution probiotic beverage are difficult to long preservation: first, use the solid mode to preserve probio, the preservation of microorganism need to be created an environmental condition that is fit to its long-term dormancy, such as low temperature, drying, anoxic, lucifuge, lack nutrition etc., make microorganism minimizing metabolic rate and the breeding that is saved and the ratio that utilizes nutrition, the solid mode is preserved probio and be can be probio and create above-mentioned dormancy condition, thereby increases substantially the holding time of probio; Second, it is 50～99.5% the assistant carrier that be used for to prolong the probio survival period that solid portion comprises mass ratio (W/W), adopt this assistant carrier can significantly improve holding time of probio, the assistant carrier for prolonging the probio survival period of the present invention comprises the mixture of a kind of, two kinds or the Multiple components of powdered glucose, maltodextrin, cane sugar powder, lactose powder, water-soluble dietary fiber, converted starch, resistant starch, corn flour, rice meal, wheat flour etc.
On the basis of technique scheme, composite beverage of the present invention also replenishes the collagen of needed by human body by two kinds of approach, the first approach is external direct absorption collagen, the present invention has added collagen in the liquid part of composite beverage, the consumer can be directly by drinking composite beverage replenishing collagen of the present invention; The second approach is synthetic collagen in the body, the present invention finds, hydroxylation step in vitamin and the human body in the collagen synthesis has certain relation, when hypovitaminosis or deficiency, can affect the synthetic of collagen, it is 0.01～10% functional component that a kind of composite beverage that contains probio and collagen of the present invention has increased mass ratio (W/W) at the solid portion of described composite beverage thus, and this functional component is vitamin A, B
1, B
2, B
6, B
12, C, D
3, among the E one or more, thereby can promote the synthetic of collagen in the body; Two kinds of approach can guarantee that beverage of the present invention is to the human body replenishing collagen thus.
Probio of the present invention can be selected in principle and anyly improve the human body microecological balance and bring into play beneficial effect, reaches the active bacteria formulation and the metabolite thereof that improve human health level and health status.For guaranteeing that probio can long preservation, probio of the present invention is Bacillus acidi lactici, saccharomycete, Bifidobacterium, two or more any compatibility in the streptococcus thermophilus, wherein Bacillus acidi lactici is lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, described Bifidobacterium is bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, these bacterial classifications all are the good purebred of typical bacterial classification, not only survival rate is high, and can keep higher biologically active after the process long preservation.
Should be noted that, the present invention does not make restriction to each bacterial classification input amount ratio, but as a design of the present invention, the present invention's suggestion is when those skilled in the art select Bacillus acidi lactici and streptococcus thermophilus as probio, preferably with streptococcus thermophilus and Bacillus acidi lactici Mixed culture, its reason is Bacillus acidi lactici energy decomposing protein, accumulation amino acid and organic acid, amino acid and organic acid can promote the growth of streptococcus thermophilus, and streptococcus thermophilus can produce Cobastab, promote the growth of Bacillus acidi lactici, if with both Mixed culture, the comparable difference of its effect is incubation growth good alone.The input ratio of streptococcus thermophilus and Bacillus acidi lactici mixed lactobacillus and streptococcus thermophilus is 1: 1.
The viable count of the probio of composite beverage of the present invention is generally 10
6～10
9The cfu/ gram.
For the nutrition that makes composite beverage of the present invention more complete, special taste, composite beverage of the present invention comprises that gross mass volume ratio (W/V) is 1.0～25% liquid part, and the on-fixed component of wherein said liquid part is two or more any compatibility in chondroitin, extract of soybean, kudzu root extract, condensed water fruit juice, fructose, HFCS, corn steep liquor, citric acid, malic acid, the essence.Wherein kudzu root extract or extract of soybean can increase bone density, replenish oestrogen-like hormone, improve skin, and chondroitin can holder in moisture content, promote bone health, lubricated joint.
Can realize that for the composite beverage that the present invention is designed the present invention also provides a kind of method for preparing composite beverage of the present invention, the method comprises solid portion preparation method and liquid part preparation method, and described solid portion preparation method comprises:
1. strain fermentation:
Adopt following bacterial strain: lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, streptococcus thermophilus, saccharomyces cerevisiae, through single strain fermentation or mixing fermentation culture, obtain zymocyte liquid, fermentation is controlled at 4～8 with the initial pH of culture medium, cultivation temperature is controlled at 30～50 ℃, and incubation time is as the criterion with fermentation termination pH to 3～6;
2. bacterium powder or probiotic microcapsule particle preparation:
After each strain fermentation finishes, get whole zymotic fluids and carry out centrifugal concentrating, get concentrated rear bacterium mud, add protective agent by 5～15% of bacterium mud mass ratio, carry out freeze drying or spray-drying after the emulsification evenly, make high concentration active microbe powder or probiotic microcapsule particle, wherein protective agent is a kind of in powdered glucose, cane sugar powder, whey powder, skimmed milk power, lactose powder, serum powder, trehalose, sodium glutamate, glycerine, dusty yeast, the maltodextrin or they arbitrarily mixed;
3. bacterium powder or probiotic microcapsule particle mix:
Two or more single strain powder or individual plant microcapsule granule are pressed living bacteria count and mass ratio is mixed, single strain powder or individual plant microcapsule granule addition are 0.05～50%, other adds mass ratio is that 0.01～10% effective component and mass ratio are 50～99.5% assistant carrier, and viable count is controlled at 10
6～10
9The cfu/ gram is prepared into the composite beverage solid portion.To add cryoprotector in freeze-drying as last, can make like this cell exempt the infringement that causes because forming ice crystal at the freezing initial stage.
The liquid part is the preparation method comprise:
(1) batching
A, title sample: take by weighing the fixedly component of liquid part, and two or more composition in the on-fixed component;
B, dissolving: the fixedly component that will take by weighing, and two or more composition in the on-fixed component adds respectively appropriate amount of purified water and carries out abundant dissolution process;
C, mixing: the single component that will dissolve respectively mixes by the priority program, stirs while adding during mixing, until till fully mixing.
(2) sterilization
Carry out sterilization treatment with membrane filtering method.
In sum, the present invention has adopted probio in the existing probiotic beverage of some solutions to be difficult to the problem of long preservation
At first, composite beverage is divided into solid portion and liquid part, and uses the mode of solid to preserve probio, created good condition for the dormancy of probio; Secondly, on this basis, select the excellent species of typical bacterial classification as the probio in the composite beverage, improved the survival rate of probio self; At last, select suitable assistant carrier, the survival period of probio has been prolonged more than one times.In addition, the present invention also utilizes some bacterial classification Mixed culture mode to turn out the higher probio of survival rate, for example, and with Bacillus acidi lactici and the streptococcus thermophilus ratio Mixed culture with 1: 1.
The specific embodiment
Understand concrete composition of the present invention for ease of those skilled in the art, the described composite beverage of present embodiment can be comprised of the component of following mass ratio:
Liquid part (W/V, %): 1.0～25
Solid portion (W/W, %): probiotics bacterial powder: 0.1～5 (containing protective agent)
Effective component: 0.01～10
Assistant carrier: 50～99.5
Wherein the collagen in the composite parts of liquid part is 1%～20% (W/V).
Wherein solid portion is prepared by following methods:
1. strain fermentation:
Adopt following bacterial strain: lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, streptococcus thermophilus, saccharomyces cerevisiae, through single strain fermentation or mixing fermentation culture; The general technical process of slant strains to shake-flask seed to ferment tank that adopt; The media components that adopts generally includes carbon source, vitamin, amino acid and the mineral matter and other components such as the nitrogenous sources such as peptone, yeast extract, beef extract, sucrose, glucose; The fermentation liquid amount is generally the 70%-80% of volume, as two kinds of bacterium or two or more shaking flask bacterial classification access respectively in the fermentation tank when adopting Mixed culture, carry out fermented and cultured, obtain zymocyte liquid, fermentation is controlled at 4～8 (acid-base modifier is 1M NaOH or 1M HCl solution) with the initial pH of culture medium, cultivation temperature is controlled at 30～50 ℃, and incubation time is as the criterion with fermentation termination pH to 3～6;
2. bacterium powder or probiotic microcapsule particle preparation:
After each strain fermentation finishes, get whole zymotic fluids and carry out centrifugal concentrating, get concentrated rear bacterium mud, add protective agent by 5～60% of bacterium mud mass ratio, carry out freeze drying or spray-drying after the emulsification evenly, make high concentration active microbe powder or probiotic microcapsule particle, wherein protective agent is a kind of in powdered glucose, cane sugar powder, whey powder, skimmed milk power, lactose powder, serum powder, trehalose, sodium glutamate, glycerine, dusty yeast, the maltodextrin or they arbitrarily mixed;
3. bacterium powder or probiotic microcapsule particle mix:
Two or more single strain powder or individual plant microcapsule granule are pressed living bacteria count and mass ratio is mixed, single strain powder or individual plant microcapsule granule addition are 0.05～50%, other adds mass ratio is that 0.01～10% effective component and mass ratio are 50～99.5% assistant carrier, and viable count is controlled at 10
6～10
9The cfu/ gram, preparation cost invention composite beverage solid portion.
For the preparation of finishing composite beverage solid portion of the present invention, and obtain the solid portion of composite beverage, can be finished by following making main points the preparation of this beverage.
Make main points: take by weighing liquid fixedly component partly by formula rate (specifically seeing embodiment), and two or more composition in the on-fixed component; With the fixedly component that takes by weighing, and two or more composition in the on-fixed component adds respectively appropriate amount of purified water and carries out abundant dissolution process; The single component of respectively dissolving is mixed by the priority program, stir while adding during mixing, until till fully mixing.Homogeneous transparent liquid with making carries out sterilization treatment with membrane filtering method, and can seals in the clean beverage bottle through sterilizing.Solid portion is after evenly mixing, and quantitative filling seals in the cavity of special plastic bottle cap.Bottle cap and beverage bottle are screwed in together, are the composite beverage (probio collagen oral liquid) that contains probio and collagen of the present invention.
Some concrete prescriptions that following embodiment is composite beverage of the present invention, the technical staff can fill a prescription according to these, and obtains composite beverage according to solid portion preparation method and the above-mentioned making main points of composite beverage of the present invention.
Embodiment
Example one: (dietary fiber)
Solid for mulation
Probio: bifidobacterium infantis 0.2%, Lactobacillus rhamnosus 0.1%, protective agent (adding by bacterium mud mass ratio 20%)
Functional component: do not add (can selectively add)
Assistant carrier: dietary fiber 70%
Liquid formulations
Fixing component: collagen 15%,
The on-fixed component: extract of soybean 0.05%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.5 * 10 in the solid
8Individual/gram, namely the shelf-life can reach 90 days at normal temperatures.
Example two: (glucose)
Solid for mulation
Probio: lactobacillus bulgaricus 0.2%, bifidobacterium bifidum 0.3%, protective agent (adding by bacterium mud mass ratio 30%)
Functional component: vitamin C 0.5%
Assistant carrier: glucose 50%
Liquid formulations
Fixing component: collagen 6%
The on-fixed component: kudzu root extract 0.1%, citric acid 1%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 2.0 * 10 in the solid
6Individual/gram, namely the shelf-life can reach 90 days at normal temperatures.
Example three: (lactose)
Solid for mulation
Probio: bifidobacterium adolescentis 0.005%, lactobacillus reuteri 0.005%, protective agent (adding by bacterium mud mass ratio 10%)
Functional component: Cobastab
62%
Assistant carrier: lactose 99%
Liquid formulations
Fixing component: collagen 10%
The on-fixed component: kudzu root extract 0.20%, corn syrup 8%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.8 * 10 in the solid
7Individual/gram, namely the shelf-life can reach 90 days at normal temperatures.
Example four: (skimmed milk power)
Solid for mulation
Probio: bifidobacterium longum 0.2%, lactobacillus acidophilus 0.05%, protective agent (adding by bacterium mud mass ratio 40%)
Functional component: vitamin E 0.030%, vitaminB10 .2%
Assistant carrier: skimmed milk power 75%
Liquid formulations
Fixing component: collagen 4%
The on-fixed component: HFCS 10%, citric acid 0.1%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 2.1 * 10 in the solid
8Individual/gram, namely the shelf-life can reach 90 days at normal temperatures.
Example five: (maltodextrin)
Solid for mulation
Probio: bifidobacterium breve 0.02%, brewer's yeast 0.02%, protective agent (adding by bacterium mud mass ratio 50%)
Functional component: vitamin A 0.018%, Cobastab
20.5%
Assistant carrier: maltodextrin 96%
Liquid formulations
Fixing component: collagen 8%
The on-fixed component: chondroitin 0.05%, HFCS 2%, fructose 6%, malic acid 0.5%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
9Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.3 * 10 in the solid
8Individual/gram, namely the shelf-life can reach 90 days at normal temperatures.
Example six: (cane sugar powder)
Solid for mulation
Probio: bifidobacterium lactis 0.2%, streptococcus thermophilus 0.3%, protective agent (adding by bacterium mud mass ratio 60%)
Functional component: vitamin B6 0.01%, vitamin D
30.018%, Cobastab
120.6%
Assistant carrier: cane sugar powder 50%
Liquid formulations
Fixing component: collagen 12%
The on-fixed component: mountain slag inspissated juice 6%, malic acid 0.5%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.3 * 10 in the solid
8Individual/gram, namely the shelf-life can reach 90 days at normal temperatures.
Example seven: (converted starch powder)
Solid for mulation
Probio: bifidobacterium lactis 0.002% (microcapsule bacterial powder), brewer's yeast 0.003%, protective agent (adding by bacterium mud mass ratio 20%)
Functional component: Cobastab
60.01%, Cobastab
121%
Assistant carrier: cane sugar powder 99.5%,
Liquid formulations
Fixing component: collagen 18%
The on-fixed component: Hami melon inspissated juice 6%, malic acid 0.5%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.6 * 10 in the solid
8Individual/gram, namely the shelf-life can reach 90 days at normal temperatures.
Should be appreciated that those skilled in the art can make various changes or modifications the present invention after having read instruction foregoing of the present invention, these equivalent form of values fall within equally the application's appended claims and limit.
Claims (3)
1. composite beverage that contains probio and collagen, partly formed by solid portion and liquid, described solid portion comprises probio at least, described liquid partly comprises fixedly component and on-fixed component, described fixedly component is collagen, it is characterized in that described solid portion also comprises for the assistant carrier that prolongs the probio survival period;
Described assistant carrier is the mixture of a kind of, two kinds or Multiple components in powdered glucose, maltodextrin, cane sugar powder, lactose powder, dietary fiber, converted starch, resistant starch, corn flour, rice meal, the wheat flour, and it is 50～99.5% the assistant carrier that is used for prolonging the probio survival period that described solid portion comprises mass ratio W/W;
Described solid portion comprises that also mass ratio W/W is that 0.1～5% to contain protectant probiotics bacterial powder, mass ratio W/W be 0.01～10% effective component, and described effective component is vitamin A, B
1, B
2, B
6, B
12, C, D
3, among the E one or more;
Probio is two or more any compatibility in Bacillus acidi lactici, saccharomycete, Bifidobacterium, the streptococcus thermophilus;
Bacillus acidi lactici is lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, and described Bifidobacterium is bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis;
The viable count of probio is 10
6～10
9The cfu/ gram;
Described on-fixed component is two or more any compatibility in chondroitin, extract of soybean, kudzu root extract, condensed water fruit juice, fructose, HFCS, corn steep liquor, citric acid, malic acid, the essence;
Wherein the mass volume ratio of liquid part in composite beverage is W/V, 1.0～25%, and the collagen W/V in the composite parts of liquid part is 1%～20%.
2. a method for preparing the described composite beverage of claim 1 is comprised of solid portion preparation method and liquid part preparation method, and described solid portion preparation method is comprised of following steps:
1. strain fermentation
Adopt following probiotics strain: lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, streptococcus thermophilus, saccharomycete, through single strain fermentation or mixing fermentation culture, obtain zymocyte liquid, fermentation is controlled at 4～8 with the initial pH of culture medium, cultivation temperature is controlled at 30～50 ℃, and incubation time is as the criterion with fermentation termination pH 3～6;
2. bacterium powder or probiotic microcapsule particle preparation
Each strain fermentation is got whole zymotic fluids and is carried out centrifugal concentrating after finishing, and gets concentrated rear bacterium mud, adds protective agents by bacterium mud mass ratio 5～15%, carries out freeze drying or spray-drying after the emulsification evenly, makes high concentration active microbe powder or probiotic microcapsule particle;
3. bacterium powder or probiotic microcapsule particle mix
Two or more single strain powder or probiotic microcapsule particle are pressed living bacteria count and mass ratio is mixed, single strain powder or individual plant microcapsule granule addition are 0.05～50%, other adds mass ratio is that 0.01～10% effective component and mass ratio are 50～99.5% assistant carrier, and viable count is controlled at 10
6～10
9The cfu/ gram is prepared into the composite beverage solid portion;
Described protective agent is a kind of in powdered glucose, cane sugar powder, whey powder, skimmed milk power, lactose powder, serum powder, trehalose, sodium glutamate, glycerine, dusty yeast, the maltodextrin or they mix arbitrarily.
3. the preparation method of described composite beverage according to claim 2, described liquid part preparation method is comprised of following steps:
⑴ batching
A, title sample: take by weighing the fixedly component of liquid part, and two or more composition in the on-fixed component;
B, dissolving: the fixedly component that will take by weighing, and two or more composition in the on-fixed component adds respectively appropriate amount of purified water and carries out abundant dissolution process;
C, mixing: the single component that will dissolve respectively mixes by the priority program, stirs while adding during mixing, until till fully mixing;
⑵ sterilization
Carry out sterilization treatment with membrane filtering method.
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| CN1887097A (en) * | 2005-06-28 | 2007-01-03 | 广东海洋大学 | Prepn technology of collagen reinforced lactic milk |
| CN1943369A (en) * | 2006-10-24 | 2007-04-11 | 辽宁优格生物科技股份有限公司 | Collagen protein milk drink and its producing method |
-
2009
- 2009-09-08 CN CN2009101953140A patent/CN101664215B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887097A (en) * | 2005-06-28 | 2007-01-03 | 广东海洋大学 | Prepn technology of collagen reinforced lactic milk |
| CN1943369A (en) * | 2006-10-24 | 2007-04-11 | 辽宁优格生物科技股份有限公司 | Collagen protein milk drink and its producing method |
Non-Patent Citations (3)
| Title |
|---|
| "胶原蛋白、维生素益生菌饮料的研制";李冰 等;《中国乳品工业》;20081231;第36卷(第1期);第32-34页 * |
| JP特开2005-225841A 2005.08.25 |
| 李冰 等."胶原蛋白、维生素益生菌饮料的研制".《中国乳品工业》.2008,第36卷(第1期),第32-34页. |
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| CN101664215A (en) | 2010-03-10 |
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