CN108113004A - The preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders - Google Patents
The preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders Download PDFInfo
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- CN108113004A CN108113004A CN201711350019.9A CN201711350019A CN108113004A CN 108113004 A CN108113004 A CN 108113004A CN 201711350019 A CN201711350019 A CN 201711350019A CN 108113004 A CN108113004 A CN 108113004A
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- lactobacillus paracasei
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- 239000006041 probiotic Substances 0.000 title claims abstract description 40
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 40
- 230000000694 effects Effects 0.000 title claims abstract description 37
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 32
- 241000186315 Lactobacillus paracasei N1115 Species 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 239000008103 glucose Substances 0.000 claims abstract description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 13
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000004108 freeze drying Methods 0.000 claims abstract description 11
- 238000003801 milling Methods 0.000 claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims description 27
- 239000000047 product Substances 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 13
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 9
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 9
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- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 4
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- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 4
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- 238000000034 method Methods 0.000 description 3
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
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- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
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- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
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- 150000007949 saponins Chemical class 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
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- 235000011497 sour milk drink Nutrition 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 229960001295 tocopherol Drugs 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- 235000010384 tocopherol Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders, including four actication of culture and the Multiplying culture, collection thalline, freeze-drying and milling steps carried out successively.Lactobacillus paracasei N1115 instant types probiotic powder provided by the present invention can still keep viable count to be not less than 10 in the later stage 18 months shelf-lifves8The vigor of CFU/g, and good mouthfeel.The present invention is used to prepare instant type probiotic powder, and blood glucose can be reduced after taking.
Description
Technical field
The invention belongs to field of food, are related to a kind of instant type probiotic powder, are a kind of high activity pair cheese specifically
The preparation method of lactobacillus N1115 instant type bacterium powders.
Background technology
Probiotics be when take in it is a certain amount of when can be to the effective living microorganisms of host health.The big portion of probiotics at present
Divide and be applied in dairy products, including products such as acidified milk, sour milk beverage, secondary cheese, milk powder, be also useful for biscuit, sugared fruit and vegetable
Fruit juice etc., in recent decades, the developed countries such as Japan, the U.S., Europe are rooted in the hearts of the people already to the concept of probiotics.At present, day
Originally, the functional fermentative lactobacillus milk drink in Europe has reached 80% in the ratio of dairy market, and North America is about 30%.In recent years
Come, to prevent thalline from dispersing, feeding is convenient and factors, the product form such as extend the shelf life are expanded to capsule, powder bag, graininess
The drying of the thalline of packaging and fermentation (breast) product or freeze-dried powder.
Although using vacuum freeze-drying method, few, the lyophilized dry bacterium powder rehydration of loss of activity of probiotics strain enzyme is good, de-
Water thoroughly, long shelf-life, storage transport and use it is all very convenient.But the crystallization of water, the mistake of cell in freezing dry process
It is very unfavorable that situations such as water, protein inactivation, so that dead or damage, the preservation, production and application for strain occurs in thalline.Therefore
The formula and preparation method of improvement instant type probiotic powder are needed, so as to keep the vigor of bacterium powder.Patent No.
201110357058.8 Chinese invention patent is related to " Lactobacillus paracasei N1115, its immunoregulation effect and application ", carries
It can be used for making bacterium powder to Lactobacillus paracasei, but without providing specific preparation method.
The content of the invention
The purpose of the present invention is to provide for a kind of preparation of high activity Lactobacillus paracasei N1115 instant type probiotic powders
Method, viable count can still be kept to be not less than 10 in the later stage 18 months shelf-lifves8The vigor of CFU/g, and good mouthfeel.
To achieve the above object, used technical solution is as follows by the present invention:
A kind of preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders, according to following step order into
Row:
(1)Actication of culture and Multiplying culture
Lactobacillus paracasei N1115 is inoculated into MRS fluid nutrient mediums and is activated;
The strain activated is inoculated in Multiplying culture in high-density culture medium, and carries out viable count detection, until viable count reaches
During 106~108CFU/ml, bacterium solution A is obtained;
(2)Collect thalline
Bacterium solution A carries out the high rotating speed of low temperature(4 DEG C, 12000rpm)Centrifugal treating, abandoning supernatant obtain bacterium mud B;
(3)Freeze-drying
Bacterium mud B is added in the solution C containing freeze drying protectant isometric with bacterium solution A, material D after mixing carries out vacuum
Freeze-drying, obtains dried frozen aquatic products E;
(4)Milling
Dried frozen aquatic products E is milled, is sieved(40 mesh)To get target product high activity Lactobacillus paracasei instant type probiotics
Powder.
As restriction, the skimmed milk containing 100~120g/L in solution C, 50~60 g/L of maltodextrin, trehalose 60~70
G/L, 15~20g/L of L-sodium, 10~15 g/L of sorbierite, 50~60g/ of whole oat four L of curing and banana puree 20~
30 g/ L.They are all the freeze drying protectant functions as thalline.
In the present invention:Lactobacillus paracasei N1115 is strain, has immunoregulation effect;
Whole oat four containing curing in the solution C containing freeze drying protectant of the present invention:Oat rich in dietary fiber, protein,
The elements such as ferrophosphorus, B family vitamin (B family vitamin is to promote metabolism in vivo, sugar, fatty, protein when changing into heat not
The substance that can lack), niacin, folic acid, pantothenic acid it is all relatively abundant, particularly vitamin E (make a living by the hydrolysate of vitamin E
Phenol is educated, tocopherol can promote sex hormone secretion to increase man's sperm motility and quantity;Increase woman's female hormone concentration,
Improve fecundity, prevention of miscarriage, it may also be used for prevention male sterility, burn, frostbite, capillary hemorrhage, climacteric are comprehensive
Close disease, beauty etc..Recently it has also been found that vitamin E can inhibit the lipid peroxidation reaction in ocular lens body, peripheral vessel is made
Expansion improves blood circulation, pre- Anti-myopic eye occurrence and development), up to 15 milligrams in every 100 grams of oatmeals.In addition oatmeal
In also containing the saponin lacked in cereal grain, (main component of ginseng has anti-aging, antifatigue, enhancing memory etc.
Effect, also with activating skin cells, the effects that enhancing skin elasticity, reducing wrinkle);The amino acid composition of protein is more complete
Face, ranking first for needed by human 8 kinds of amino acid contents, is especially up to 0.68 gram containing lysine;Whole oat four is carried out
The purpose of curing is to be easier to absorb(Whole oat four is if not done by curing, the not easily digested suction of the starch included
It receives), contain oligofructose in oat, the content of the whole oat four oligofructose in 50~60g/ L has thalline good holding
The effect of activity.
Contain banana puree in solution C in the present invention:Banana contains the phosphorus of referred to as " salt of wisdom ", and has abundant albumen
Matter, sugar, potassium, vitamin A and C, while fiber is also more, can be rated as fairly good nutraceutical.Every 100 grams of banana pulp is containing sugar 15%
More than, acid 0.2~0.3%, protein 1.5%, also 53 milligrams abundant of phosphorus, 19 milligrams of calcium, 400 milligrams of potassium, 4 milli of Catergen
Gram.Banana is also containing pectin, a variety of enzyme materials and trace element etc.;Contain oligofructose, the content of banana puree in banana
In 20~30 g/ L, oligofructose and oat cooperation play the role of thalline to keep activity well.
The present invention makes probiotics exist using oat and the rational proportion of banana, the frozen-dried protective liquid ingredient for probiotics of arranging in pairs or groups
Damage in freeze-drying process is reduced, and freezes survival rate rise, while is found in the Detection of Stability of shelf-life, probiotic powder
Vigor attenuation rate reduces, and substantially increases the stability and validity of bacterium powder.
As a kind of restriction of the present invention, above-mentioned steps(1)Described in high-density culture medium 20g/L containing glucose, ferment
Female powder 20g/L, buffer salt 0.09mol/L, MnSO4 5H2O 50mg/L.Glucose, dusty yeast, buffer salt, MnSO4 5H2O
It can be grown for N1115 and necessary element is provided, promote its High Density Cultivation.
Another as the present invention limits, above-mentioned steps(3)Described in vacuum freeze drying, according to following steps according to
Secondary progress:
1. pre-freeze
Material D is subjected to pre-freeze under conditions of -45~-40 DEG C of flaggy temperature, until the temperature of material reaches -35 DEG C or less
When, material D product freeze reality completely;Subsequently into
2. primary drying phase
It is that temperature is to slowly warm up to -30~-26 DEG C by 5 DEG C/30min with heating rate, maintains this temperature until flaggy and product
Between heat exchange terminate substantially, vacuum degree is had descended to less than or equal to 0.02MPa megapascal, and fluctuating range is less than or equal to
0.002, -70 DEG C are down at a temperature of cold well, and primary drying phase terminates;Subsequently into
3. the parsing-desiccation stage
Temperature is directly raised to 10 DEG C, is evaporated the moisture of remaining, until water content is 3.5~4%, vacuum freeze drying terminates.
The present invention is as a result of above-mentioned technical solution, and compared with prior art, acquired technological progress is:
(1)The present invention passes through high-density culture medium in actication of culture and Multiplying culture;The cell concentration of bacterium mud can be improved, from
And improve the yield of bacterium powder.
(2)In the preparation process of the present invention, freeze drying protectant ingredient and oat, banana are added, thalline can be protected, simultaneously
Keep the vigor in the bacterium powder shelf-life;
(3)High activity Lactobacillus paracasei N1115 instant types probiotic powder obtained by the present invention is taken available for instant, also may be used
With warm water, warm milk, 2 times can be taken within one day, one time 2 grams, continuously take 3 months, there is the function for reducing blood glucose.Pair is dry
Lactobacillus paracasei N1115 instant type probiotic powders are deposited at shady and cool drying, and more preferably, the shelf-life is 18 months for refrigeration.
The present invention is used to prepare high activity instant type probiotic powder, and blood glucose can be reduced after taking.
Description of the drawings
Fig. 1 is the made Lactobacillus paracasei N1115 instant type probiotic powders of embodiment 1-6 in the embodiment of the present invention 7 18
Number of viable testing result figure in a month.
Specific embodiment
The present invention is described in further detail below in conjunction with specific embodiment, and following embodiment is only to the present invention
Supplementary notes, are not any type of restrictions made for the present invention.
The preparation method of embodiment 1-6 high activity Lactobacillus paracasei N1115 instant type probiotic powders
Embodiment 1-6 is respectively a kind of preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders, they are pressed
It is carried out according to following step order:
(1)Actication of culture and Multiplying culture
Lactobacillus paracasei N1115 is inoculated into MRS fluid nutrient mediums and is activated;
The strain activated is inoculated in high-density culture medium(20g/L containing glucose, dusty yeast 20g/L, buffer salt are
0.09mol/L、MnSO4•5H2O 50mg/L)Middle Multiplying culture, and viable count detection is carried out, until viable count reaches 106-
During 108CFU/ml, bacterium solution A is obtained;
(2)Collect thalline
Bacterium solution A is carried out to the high rotating speed centrifugal treating of low temperature under 4 DEG C, 12000rpm, abandoning supernatant obtains bacterium mud B;
(3)Freeze-drying
Bacterium mud B is added in and solution C isometric bacterium solution A(Solution containing freeze drying protectant, specifically takes off containing 100~120g/L
Fat breast, 50~60g/L of maltodextrin, 60~70g/L of trehalose, 15~20g/L of L-sodium, 10~15g/L of sorbierite,
50~60g/L of whole oat four and banana puree the 20-30 g/L of curing)In, 1. material D after mixing is carried out by following steps
~the vacuum freeze drying 3. completed successively:
1. pre-freeze
Material D is subjected to pre-freeze under conditions of -45~-40 DEG C of flaggy temperature, until the temperature of material reaches -35 DEG C or less
When, material D product freeze reality completely;Subsequently into
2. primary drying phase
It is that temperature is to slowly warm up to -30~-26 DEG C by 5 DEG C/30min with heating rate, maintains this temperature until flaggy and product
Between heat exchange terminate substantially, vacuum degree is had descended to less than or equal to 0.02 megapascal, and fluctuating range is less than or equal to
0.002, -70 DEG C are down at a temperature of cold well, and primary drying phase terminates;Subsequently into
3. the parsing-desiccation stage
Temperature is directly raised to 10 DEG C, is evaporated the moisture of remaining, until water content is 3.5~4%, vacuum freeze drying terminates,
Obtain dried frozen aquatic products E;
(4)Milling:Dried frozen aquatic products E is milled, is sieved to get target product high activity Lactobacillus paracasei instant type probiotics
Powder.
Obtained high activity Lactobacillus paracasei N1115 instant types probiotic powder is taken available for instant, also can be with temperature
Water, warm milk, can take 2 times for one day, one time 2 grams, continuously take 3 months, there is the function for reducing blood glucose.High activity pair
Lactobacillus casei N1115 instant type probiotic powders are deposited at shady and cool drying, and more preferably, the shelf-life is 18 months for refrigeration.
The preparation method of the high activity Lactobacillus paracasei N1115 instant type probiotic powders of 7 embodiment 1-6 of embodiment
The middle advantageous effect contrast test using high-density culture medium
The present embodiment is tested by comparison of design, to using high-density culture medium in actication of culture and Multiplying culture, to made
The advantageous effect that the bacterium powder number of viable obtained can be generated, is studied, specific result of the test is as follows:
As it can be seen that using high-density culture medium in actication of culture and Multiplying culture, the cell concentration of bacterium mud can be improved, so as to carry
The yield of high bacterium powder.
In the 8 embodiment 1-6 institutes preparing high-activity Lactobacillus paracasei N1115 instant type probiotic powder shelf-lifves of embodiment
Viable count detection experiment
The present embodiment various high activity Lactobacillus paracasei N1115 instant type probiotic powders made to embodiment 1-6 are at 18
Number of viable in month has carried out tracing detection, and testing result shows high activity pair cheese breast provided by the present invention with reference to figure 1
For bacillus N1115 instant types probiotic powder within the shelf-life of 18 months, number of viable is in be gradually reduced trend, but in 18 months phases
10 are remained at when full8More than cfu/g.
9 embodiment 1-6 institutes preparing high-activity Lactobacillus paracasei N1115 instant types probiotic powder of embodiment improves human body sugar
The experiment of tolerance
It is resistance to that the present embodiment improves human body sugar to embodiment 1-6 institutes preparing high-activity Lactobacillus paracasei N1115 instant types probiotic powder
The situation of amount devises test-meal experiment:
1st, 10 impaired glucose tolerance subjects 10 are screened.I.e. fasting blood-glucose is not reaching to diabetes diagnostic criterion, i.e.,
Below 7.0mmol/L, while 75g standard glucose tolerance tests(OGTT)2h blood glucose >=7.8mmol/L and < 11.1mmol/L.
2nd, simple randomization is divided into 5 people of intervention group, and 5 people of control group takes the instant probiotic powders of N1115 and placebo daily
(Maltodextrin).Dose is daily 2 bags, 2g/ bags, and viable count is up to 10 in instant bacterium powder10CFU/g.It takes 3 months.
3rd, respectively in the early stage, latter stage detection subject fasting blood-glucose and OGTT experiment after blood glucose target.
4th, result such as following table:
Blood glucose target describes substantially(Mean ± standard deviation)
In upper table:Intervention group is with compareing group difference unobvious in the early stage for fasting blood-glucose, but latter stage intervention group fasting blood-glucose declines,
Difference has statistical significance.OGTT experiment clothes sugar after 2 it is small when blood glucose initial stage level it is not notable with compareing group difference in intervention group,
After intervention group latter stage OGTT experiment clothes sugar 2 it is small when blood glucose level declined compared with baseline and difference is statistically significant.
Conclusion (of pressure testing):It is believed that high activity Lactobacillus paracasei N1115 instant type probiotic powders, have the machine that has some improvement
The effect of body sugar tolerance.
Claims (5)
1. a kind of preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders, it is characterised in that:It according to
Under step order carry out:
(1)Actication of culture and Multiplying culture
Lactobacillus paracasei N1115 is inoculated into MRS fluid nutrient mediums and is activated;
The strain activated is inoculated in Multiplying culture in high-density culture medium, and carries out viable count detection, until viable count reaches
106~108During CFU/ml, bacterium solution A is obtained;
(2)Collect thalline
Bacterium solution A carries out the high rotating speed centrifugal treating of low temperature, and abandoning supernatant obtains bacterium mud B;
(3)Freeze-drying
Bacterium mud B is added in the solution C containing freeze drying protectant isometric with bacterium solution A, material D after mixing carries out vacuum
Freeze-drying, obtains dried frozen aquatic products E;
(4)Milling
Dried frozen aquatic products E is milled, is sieved to get target product high activity Lactobacillus paracasei instant type probiotic powder.
2. the preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders according to claim 1, special
Sign is:The skimmed milk containing 100~120g/L in solution C, 50~60 g/L of maltodextrin, 60~70 g/L, L- paddy ammonia of trehalose
Sour 15~20g/L of sodium, 10~15 g/L of sorbierite, 20~30 g/ L of 50~60g/ of whole oat four L and banana puree of curing.
3. the preparation method of Lactobacillus paracasei N1115 instant type probiotic powders according to claim 2, feature exist
In:Also contain skimmed milk, maltodextrin, trehalose, L-sodium and sorbierite 10-15 in the solution C.
4. the system of high activity Lactobacillus paracasei N1115 instant type probiotic powders according to any one of claim 1-3
Preparation Method, it is characterised in that:The step(1)In, high-density culture medium 20g/L containing glucose, dusty yeast 20g/L, buffer salt
For 0.09mol/L, MnSO4•5H2O 50mg/L。
5. the system of high activity Lactobacillus paracasei N1115 instant type probiotic powders according to any one of claim 1-3
Preparation Method, it is characterised in that:Step(3)Described in vacuum freeze drying, carried out successively according to following steps:
1. pre-freeze
Material D is subjected to pre-freeze under conditions of -45~-40 DEG C of flaggy temperature, until the temperature of material reaches -35 DEG C or less
When, material D product freeze reality completely;Subsequently into
2. primary drying phase
It is that temperature is to slowly warm up to -30~-26 DEG C by 5 DEG C/30min with heating rate, maintains this temperature until flaggy and system
Heat exchange between product terminates substantially, and vacuum degree is had descended to less than or equal to 0.02MPa, and fluctuating range is less than or equal to
0.002, -70 DEG C are down at a temperature of cold well, and primary drying phase terminates;Subsequently into
3. the parsing-desiccation stage
Temperature is directly raised to 10 DEG C, is evaporated the moisture of remaining, until water content is 3.5~4%, vacuum freeze drying terminates.
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CN109479961A (en) * | 2018-08-31 | 2019-03-19 | 石家庄君乐宝乳业有限公司 | Lactobacillus paracasei N1115 prevents application and the corresponding baby milk powder, application of colitis |
CN109479972A (en) * | 2018-12-10 | 2019-03-19 | 石家庄君乐宝乳业有限公司 | Improve the baby milk powder companion and preparation method thereof of gastrointestinal tract |
CN109997921A (en) * | 2019-03-29 | 2019-07-12 | 石家庄君乐宝乳业有限公司 | Fresh cheese food and preparation method thereof |
CN110464005A (en) * | 2019-09-23 | 2019-11-19 | 石家庄君乐宝乳业有限公司 | Composition and preparation method thereof suitable for patients with diabetes mellitus |
CN110604294A (en) * | 2019-09-23 | 2019-12-24 | 石家庄君乐宝乳业有限公司 | Preparation method of N1115 probiotic powder suitable for diabetics and application of corresponding probiotic powder |
CN110604781A (en) * | 2019-09-23 | 2019-12-24 | 石家庄君乐宝乳业有限公司 | Lactobacillus paracasei N1115-containing bacterial powder capable of preventing and relieving diabetes, and preparation method and application thereof |
CN112458029A (en) * | 2020-12-21 | 2021-03-09 | 苏州微克生活科技有限公司 | Preparation method and application of high-activity lactobacillus paracasei freeze-dried powder |
WO2022115035A1 (en) * | 2020-11-24 | 2022-06-02 | Nanyang Technological University | Probiotic compositions and methods of fermentation thereof |
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CN110604781A (en) * | 2019-09-23 | 2019-12-24 | 石家庄君乐宝乳业有限公司 | Lactobacillus paracasei N1115-containing bacterial powder capable of preventing and relieving diabetes, and preparation method and application thereof |
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CN112458029A (en) * | 2020-12-21 | 2021-03-09 | 苏州微克生活科技有限公司 | Preparation method and application of high-activity lactobacillus paracasei freeze-dried powder |
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