CN103013970A - Immobilized probiotics both rich in plant fiber and probiotics and preparation method thereof - Google Patents
Immobilized probiotics both rich in plant fiber and probiotics and preparation method thereof Download PDFInfo
- Publication number
- CN103013970A CN103013970A CN2012105010710A CN201210501071A CN103013970A CN 103013970 A CN103013970 A CN 103013970A CN 2012105010710 A CN2012105010710 A CN 2012105010710A CN 201210501071 A CN201210501071 A CN 201210501071A CN 103013970 A CN103013970 A CN 103013970A
- Authority
- CN
- China
- Prior art keywords
- probiotic bacterium
- rich
- immobilization
- vegetable fibre
- cereal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to immobilized probiotics both rich in plant fibers and probiotics and a preparation method thereof. The immobilized probiotics are microcapsule powder obtained by embedding the probiotics by taking the pure grain powder of the plant fibers as embedding materials. The raw materials of the immobilized probiotics comprise the following components in percentage by weight: 70%-80% of pure grain seeds and 20%-30% of thallus culture solutions. Compared with the prior art, the immobilized probiotics disclosed by the invention has the advantages of both supplementing the plant fiber and the probiotics, and the like.
Description
Technical field
The present invention relates to a kind of immobilization probiotic bacterium, especially relate to a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium and preparation method thereof.
Background technology
Probiotic bacterium (Probiotics) is a kind of beneficial microorganism that can improve, nurse one's health human intestinal microbial balance, and its physiologic function obtains whole world scientific and technological circle and generally admits and be widely used in medicine, health care and food aspect.Vegetable fibre is a kind of carbohydrate that can not be digested by human body, and it has very important effect to promoting good digestion and draining solid waste.Vegetable fibre can make the food in the enteron aisle increase deliquescing, promotes intestines peristalsis, thereby accelerates defecation speed, prevents constipation and reduces the intestinal cancer risk.It is the indispensable the seventh-largest nutrient substance of human body.Grain flour embedded material take vegetable fibre as main nutrient elements is made the immobilization probiotic bacterium can make vegetable fibre and probiotic bacterium act on simultaneously human intestinal, and the dual function of two kinds of nutritive elements more is conducive to balance, improves human intestinal health.
Because at present probiotic bacterium adopts usually that the inorganic materials such as silicon-dioxide, activated alumina, titanium dioxide, diatomite or its are material modified to be carrier; And the natural organic high-molecular solid support material is as embedded material.The major defect of these embedded materials has: 1. these artificial polymkeric substance that are used for food have many uncertain factors, and existing natural organic high-molecular solid support material has physical strength lower, poor flexibility, under anaerobic easily decomposed by microorganism, can cause the defectives such as variation of mechanisms for drug release; 2. these artificial polymkeric substance can restrict the use range of live body probiotic bacterium greatly; 3. the immobilization probiotic bacterium of making take artificial polymkeric substance as embedded material can't be satisfied the demand of healthy enteron aisle because it contains a kind of nutritive element of profitable probliotics comprehensively.
Because the defective of above manufacture craft can't satisfy the existing market demand.And the grain flour embedded material take vegetable fibre as main nutrient elements makes the method for immobilization probiotic bacterium and not only overcome the defective that prior art exists, and also changed to use artificial polymkeric substance to be the form of probiotic bacterium carrier at present.The food raw material that vegetable fibre is rich in utilization is the embedding equivalent material, and this material not only can directly be eaten; Abundant vegetable fibre can improve and protect intestinal environment, and this grain flour embedded material that is rich in vegetable fibre can provide enough nutrients for probiotic bacterium simultaneously, strengthens its activity after entering enteron aisle; Intestinal health can effectively be takeed good care of, be improved, protect to the double effects of probiotic bacterium and vegetable fibre.
Summary of the invention
Purpose of the present invention is exactly to provide a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium and preparation method thereof for the defective that overcomes above-mentioned prior art existence.
Purpose of the present invention can be achieved through the following technical solutions: a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium, it is characterized in that, the microscapsule powder of this immobilization probiotic bacterium for obtaining as embedded material embedding probiotic bacterium take the pure grain flour that is rich in vegetable fibre, its starting material comprise the component of prescription:
Pure cereal seed: 70～80wt%;
Bacterial culture fluid: 20～30wt%.
Described pure cereal seed comprises one or more in soybean, wheat, the rice.
Described pure cereal seed is mixed with mass ratio by soybean, wheat, rice at 7: 2: 1.
Described bacterial culture fluid is to add the milk powder 1.5wt% that contains probiotic bacterium, potassium hydrogen phosphate 0.06wt%, vitamins B in water
20.01wt%, the rear sterilization that stirs makes.
Described bacterial culture fluid is the aqueous solution of commercially available probiotic bacterium, and mass percent concentration is 1-2%.
The particle diameter of described microcapsule is 1～5 μ m.
A kind of preparation method who is rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium, it is characterized in that, the method may further comprise the steps: 8-12h at normal temperatures is soaked in water pure cereal seed, defibrination, centrifugation macrobead throw out again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed, regulating the pH value is 7.2-7.5, surface of microcapsule is electronegative in the solution after self-emulsifying is processed, the cereal colloidal solution that obtains is become fine-particle powder, being fixed probiotic products with bacterial culture fluid mixing postlyophilization.
The cereal colloidal solution that described cereal colloidal solution is solid 40%, the viable count of described bacterial culture fluid＞1000/ gram.
The pH value that described self-emulsifying is treated to the insoluble composition of regulating described soluble part and suspending is 7-8, and in homogenizer, stir 10-30min, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself; Described cryodesiccated temperature is-40 ℃～0 ℃.
Described pH value adopts sodium bicarbonate to regulate.
Compared with prior art, the present invention has the following advantages:
1, the embedded material itself that is rich in vegetable fibre is the nutrient of probiotic bacterium, can guarantee the long period of activity of probiotic bacterium;
2, the combination of probiotic bacterium and vegetable fibre can make the effect maximization that improves enteron aisle;
3, products obtained therefrom is that immobilization material is direct-edible;
4, products obtained therefrom is that immobilization material itself is food, and is safe and reliable;
5, products obtained therefrom is the immobilization material convenient sources.
Description of drawings
Fig. 1 is the Electronic Speculum figure of immobilization probiotic products of the present invention.
Embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1 (lactic bacteria activity powder-product)
Grain flour embedded material take vegetable fibre as main nutrient elements is made the method for immobilization probiotic bacterium, and the method comprises following processing step:
1. the mixture that mixes at 7: 2: 1 in mass ratio of soybean wheat rice is made living lactic acid bacteria powder product as investment;
2. after getting at normal temperatures step (1) gained mixture 70kg and being soaked in water 8-12 hour, defibrination, centrifugation macrobead throw out composition again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed, namely adopt sodium bicarbonate to regulate described soluble part and the pH value of the insoluble composition that can suspend is 7.2, and in homogenizer, stirred 15 minutes, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself, and to regulate pH be that surface of microcapsule is electronegative in 7.2 solution that make after self-emulsifying is processed, and produces solid substance and be 40% colloidal solution;
3. add lactic acid bacteria culture solution 30kg at resulting colloidal solution at last, at-40 ℃ of lower being fixed of vacuum-drying probiotic bacterium pulvis products, this product is microencapsulated after mixing, and the particle diameter of microcapsule is 1～5 μ m, and the Electronic Speculum figure of products obtained therefrom as shown in Figure 1.
Described bacterial culture fluid is: add the milk powder 1.5wt% that contains the lactic bacteria activity powder in water, potassium hydrogen phosphate 0.06wt% Lin Suanna Vitamin B2 Sodium Phosphate 0.01wt% stirs evenly rear sterilization and makes.
Embodiment 2 (the dried bacterium of two qis)
Grain flour embedded material take vegetable fibre as main nutrient elements is made the method for immobilization probiotic bacterium, and the method comprises following processing step:
1. the mixture that mixes at 7: 3 in mass ratio take soybean and rice is as raw material,
Get be soaked in water 8-12 hour under the described mixture 80kg of above-mentioned steps (1) normal temperature after, defibrination, centrifugation insoluble composition again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed: the pH value that adopts sodium bicarbonate to regulate described soluble part is 7.5, and in homogenizer, stirred 20 minutes, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself;
3. adopting sodium bicarbonate to regulate pH is the electronegative colloidal solution that obtains of surface of microcapsule in 7.5 solution that make after self-emulsifying is processed;
4. the last bacterial culture fluid 20kg that adds in the colloidal solution that obtains mixes rear at the probiotic bacterium of being fixed of vacuum-drying below-30 ℃ pulvis.
Described bacterial culture fluid is for adding the milk powder 1.5wt% that contains two dried bacterium of qi in water, potassium hydrogen phosphate 0.06wt% Lin Suanna Vitamin B2 Sodium Phosphate 0.01wt% stirs evenly rear sterilization and makes.
Embodiment 3 (milk-acid bacteria+dried bacterium+yeast of two qis)
Grain flour embedded material take vegetable fibre as main nutrient elements is made the method for immobilization probiotic bacterium, and the method comprises following processing step:
1. the mixture that mixes at 8: 2 in mass ratio take soybean and rice is as raw material;
2. after going the described mixture 75kg of step (1) to be soaked in water at normal temperatures 8-12 hour, defibrination, again centrifugation insoluble composition;
3. the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed, and regulates pH and make in the solution after self-emulsifying is processed surface of microcapsule electronegative;
4. add probiotic's culture liquid 25kg (the dried bacterium of milk-acid bacteria+two qis+saccharomycetes to make fermentation liquid makes viable bacteria finally reach 1,000 hundred million/gram) at the colloidal solution that obtains at last, solid substance is transferred to lyophilize below 40%, 0 ℃ make immobilization probiotic bacterium pulvis.
Described bacterial culture fluid contains milk-acid bacteria, the dried bacterium of two qi and saccharomycetic milk powder 1.5wt% for adding in water, potassium hydrogen phosphate 0.06wt% Lin Suanna Vitamin B2 Sodium Phosphate 0.01wt% stirs evenly rear sterilization and makes.
Described self-emulsifying is treated to protein in the cereal is transferred to meta-alkalescence, stirs under the effect of high speed shear power with the contained grease of cereal itself and forms albumen-grease microcapsule in 10 minutes.
Described adjusting pH adopts sodium bicarbonate, and it is 7.4 that adjusting pH makes the pH value of the solution after self-emulsifying is processed.
Embodiment 4
Grain flour embedded material take vegetable fibre as main nutrient elements is made the method for immobilization probiotic bacterium, and the method comprises following processing step:
Take soybean as raw material;
Get be soaked in water 8-12 hour under the described soybean 78kg of above-mentioned steps (1) normal temperature after, defibrination, centrifugation insoluble composition again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed: the pH value that adopts sodium bicarbonate to regulate described soluble part is 7.5, and in homogenizer, stirred 20 minutes, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself;
7. adopting sodium bicarbonate to regulate pH is the electronegative colloidal solution that obtains of surface of microcapsule in 7.5 solution that make after self-emulsifying is processed;
8. the last bacterial culture fluid 22kg that adds in the colloidal solution that obtains mixes rear at the probiotic bacterium of being fixed of vacuum-drying below-30 ℃ pulvis.
Described bacterial culture fluid is the aqueous solution of commercially available probiotic bacterium, and the probiotic bacterium mass percent concentration is 1-2%.
Claims (10)
1. an immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium is characterized in that, the microscapsule powder of this immobilization probiotic bacterium for obtaining as embedded material embedding probiotic bacterium take the pure grain flour that is rich in vegetable fibre, and its starting material comprise the component of prescription:
Pure cereal seed: 70～80wt%;
Bacterial culture fluid: 20～30wt%.
2. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 is characterized in that, described pure cereal seed comprises one or more in soybean, wheat, the rice.
3. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 and 2 is characterized in that, described pure cereal seed is mixed with mass ratio by soybean, wheat, rice at 7: 2: 1.
4. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 is characterized in that, described bacterial culture fluid is to add the milk powder 1.5wt% that contains probiotic bacterium, potassium hydrogen phosphate 0.06wt%, vitamins B in water
20.01wt%, the rear sterilization that stirs makes.
5. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 is characterized in that, described bacterial culture fluid is the aqueous solution of commercially available probiotic bacterium, and mass percent concentration is 1-2%.
6. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 is characterized in that, the particle diameter of described microcapsule is 1～5 μ m.
7. preparation method who is rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium as claimed in claim 1, it is characterized in that, the method may further comprise the steps: 8-12h at normal temperatures is soaked in water pure cereal seed, defibrination, centrifugation macrobead throw out again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed, regulating the pH value is 7.2-7.5, surface of microcapsule is electronegative in the solution after self-emulsifying is processed, the cereal colloidal solution that obtains is become fine-particle powder, being fixed probiotic products with bacterial culture fluid mixing postlyophilization.
8. the preparation method who is rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium according to claim 7, it is characterized in that, the cereal colloidal solution that described cereal colloidal solution is solid 40%, the viable count of described bacterial culture fluid＞1000/ gram.
9. the preparation method who is rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium according to claim 7, it is characterized in that, the pH value that described self-emulsifying is treated to the insoluble composition of regulating described soluble part and suspending is 7-8, and in homogenizer, stir 10-30min, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself; Described cryodesiccated temperature is-40 ℃～0 ℃.
10. according to claim 7 or the 9 described preparation methods that are rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium, it is characterized in that, described pH value adopts sodium bicarbonate to regulate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105010710A CN103013970A (en) | 2012-11-29 | 2012-11-29 | Immobilized probiotics both rich in plant fiber and probiotics and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105010710A CN103013970A (en) | 2012-11-29 | 2012-11-29 | Immobilized probiotics both rich in plant fiber and probiotics and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103013970A true CN103013970A (en) | 2013-04-03 |
Family
ID=47963085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105010710A Pending CN103013970A (en) | 2012-11-29 | 2012-11-29 | Immobilized probiotics both rich in plant fiber and probiotics and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103013970A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018082665A1 (en) * | 2016-11-04 | 2018-05-11 | 华为技术有限公司 | Frequency synchronization method and slave clock |
| CN108113004A (en) * | 2017-12-15 | 2018-06-05 | 石家庄君乐宝乳业有限公司 | The preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010016220A1 (en) * | 1999-12-22 | 2001-08-23 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Encapsulated multifunctional biologically active food component, process for its production and its use |
| CN101534658A (en) * | 2006-10-06 | 2009-09-16 | 先进生物技术学院 | Pre-fermented symbiotic matrix based on a cereal suspension with encapsulated probiotics, manufacture process and corresponding uti lization |
| CN101933620A (en) * | 2009-07-01 | 2011-01-05 | 何黎明 | Probiotic powder and preparation method |
| CN102919669A (en) * | 2012-10-12 | 2013-02-13 | 上海致一食品有限公司 | Method for preparing immobilization probiotics by taking grain powder as embedding material |
-
2012
- 2012-11-29 CN CN2012105010710A patent/CN103013970A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010016220A1 (en) * | 1999-12-22 | 2001-08-23 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Encapsulated multifunctional biologically active food component, process for its production and its use |
| CN101534658A (en) * | 2006-10-06 | 2009-09-16 | 先进生物技术学院 | Pre-fermented symbiotic matrix based on a cereal suspension with encapsulated probiotics, manufacture process and corresponding uti lization |
| CN101933620A (en) * | 2009-07-01 | 2011-01-05 | 何黎明 | Probiotic powder and preparation method |
| CN102919669A (en) * | 2012-10-12 | 2013-02-13 | 上海致一食品有限公司 | Method for preparing immobilization probiotics by taking grain powder as embedding material |
Non-Patent Citations (1)
| Title |
|---|
| 谭斌: "功能性谷物基质非乳性益生菌的研究展望", 《中国食品添加剂》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018082665A1 (en) * | 2016-11-04 | 2018-05-11 | 华为技术有限公司 | Frequency synchronization method and slave clock |
| CN108113004A (en) * | 2017-12-15 | 2018-06-05 | 石家庄君乐宝乳业有限公司 | The preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104431621B (en) | A kind of aquatic products compound premix and preparation method thereof | |
| CN106165764A (en) | One improves meat enzymolysis laminaria production fermented grain type tilapia feed | |
| CN105249019A (en) | Young shrimp feed and preparation method thereof | |
| CN107594109A (en) | A kind of pet drinks enzyme liquid and preparation method thereof | |
| CN106993788A (en) | All Pure Nature royal jelly lozenge and preparation method thereof | |
| CN103013970A (en) | Immobilized probiotics both rich in plant fiber and probiotics and preparation method thereof | |
| CN103875977A (en) | Mixed feed for aquaculture | |
| CN113100345A (en) | Pig feed additive for improving immunity and preparation method of pig feed | |
| CN102805150A (en) | Live bacteria type lotus seed and corn yoghurt beverage and preparation method thereof | |
| CN102379391A (en) | Preparation of health food of pine pollen, glucan and natto | |
| CN103907785B (en) | A kind of atherosclerotic slaking breast piglet biological feedstuff and preparation method thereof | |
| CN102919669B (en) | Method for preparing immobilization probiotics by taking grain powder as embedding material | |
| CN107361355A (en) | A kind of preparation method of jujube ferment powder | |
| CN105309844A (en) | Efficient feed capable of promoting immunity of penaeus vannamei boone and preparation method | |
| CN108157606A (en) | A kind of feed addictive for promoting growth | |
| CN107348231A (en) | A kind of eel feed and preparation method thereof | |
| CN106923117A (en) | A kind of preparation method of the asparagus fermented beverage with brain tonic and intelligence development effect | |
| CN112021462A (en) | Bionical system of liquid pig milk of mainly utilizing vegetable protein production | |
| CN106265412A (en) | A kind of method utilizing probiotics fermention to prepare Margarita hydrolyzed solution | |
| CN101928676A (en) | Method for fermenting animal edible lactobacillus | |
| CN108850469A (en) | A kind of natto bacillus feed additive | |
| CN108684972A (en) | Improve the egg feedstuff of egg laying performance | |
| CN108402308A (en) | A kind of feeding complex microorganism additive | |
| LU100946B1 (en) | Pollen-based nutritional paste and preparation procedure | |
| CN108850438A (en) | A kind of composite bacillus feed addictive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130403 |