CN108850438A - A kind of composite bacillus feed addictive - Google Patents
A kind of composite bacillus feed addictive Download PDFInfo
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- CN108850438A CN108850438A CN201810511732.5A CN201810511732A CN108850438A CN 108850438 A CN108850438 A CN 108850438A CN 201810511732 A CN201810511732 A CN 201810511732A CN 108850438 A CN108850438 A CN 108850438A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/24—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants
- B65D81/26—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators
- B65D81/266—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators for absorbing gases, e.g. oxygen absorbers or desiccants
- B65D81/268—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators for absorbing gases, e.g. oxygen absorbers or desiccants the absorber being enclosed in a small pack, e.g. bag, included in the package
Abstract
A kind of composite bacillus feed addictive of the present invention, it is characterized in that being uniformly mixed by bacillus licheniformis, bacillus subtilis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, the fermentation egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made：Bacillus licheniformis 5000 hundred million -60000 hundred million in every 1000g feed addictive, bacillus subtilis 5000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is fermentation egg-shell meal.The present invention is clear containing viable count, application and Quantitatively Selecting conducive to user.The present invention is using sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder and fermentation egg-shell meal as the matrix of additive, not only improve the nutritive value of composite bacillus additive, promote the metabolism of animal simultaneously, improve the disease resistance of body, promote growth of animal, finally improves the economic benefit of its aquaculture.
Description
Technical field
The invention belongs to feed additive fields, are related to a kind of bacillus feed additive for cultivated animals.Tool
Body is related to a kind of composite bacillus feed addictive.
Background technique
Bacillus mainly takes oxygen by force by biology in the gastrointestinal tract and maintains intestinal eubiosis, it is colonized in short-term in enteron aisle
Afterwards, a large amount of oxygen can be consumed and maintain enteron aisle anaerobic environment, field planting power of the enhancing enteron aisle to anaerobic bacteria.In addition, bacillus
It can be monosaccharide by Starch Conversion in enteron aisle, then convert lactic acid for these monosaccharide by strains other in enteron aisle, reduce enteron aisle
PH, thus play the role of inhibit pathogen.
Probiotic composition made of bacillus and other microorganisms has a significant difference, bacillus can condition not
Gemma is formed in the case where benefit, will be protected self, and resurrection rate is high.Raw spore form exists within such product, have compared with
More advantages：1. acidproof, salt tolerant, high temperature resistant（100℃）And anti-extrusion, thus pelletize, in the processing of feed by animal gastric acid
Stability with higher during environment etc.；2. the activity with very strong protease, lipase, amylase, to vegetalitas
Carbohydrate has stronger degradation capability, and the compound of certain complexity in energy degrading plant feed, generating has antagonism
The polypeptides matter of pathogenic entero becteria ability.This bacteroid has drop to the carbohydrate of many complexity of plant component part
Solution ability, the substance that can be degraded have triglycerides, protein, starch, xylan, β-Isosorbide-5-Nitrae-galactan, pectin, carboxymethyl
Cellulose, polygalacturonase, araban, glucose, p-nitrophenyl-glucopyranoside, p-nitrophenyl-Arab
Furanoside, lichenin and p-nitrophenyl-furyl xylose etc..
Bacillus licheniformis and bacillus subtilis are all that Minister Agriculture of China announces No. 2045 announcement《Feed addictive
Catalogue（2013）》It is allowed for the microorganism of cultivated animals, with regard to current bacillus licheniformis and bacillus subtilis as addition
The dosage form that agent uses has aqua and pulvis two major classes, and aqueous solution dosage form is because of the nutritional substance in thallus aqua easy to use
Continue to be metabolized, growth fermentation, therefore, the thallus of this dosage form plays pendulum, and storage life is shorter.Powder formulation be then by
Thallus or gemma are dehydrated at a slow speed using drying means such as low temperature hot wind, freezings, are in thallus or gemma by thallus living
Stable drying dormant state recycles substance that can be feeding to be uniformly mixed as filler and relatively stable pulvis is made
Type biological feed additive.But with regard to current manufacturing enterprise in view of raw material be easy to get and processing cost, often select rice bran, bran
One of skin, corn flour, maize cob meal, peanut shell powder, conch meal, vermiculite power, mountain flour, calcium carbonate, medical stone, zeolite powder or
Several mixtures, some also use plant straw powder or grass meal as filler material.The considerations of these are in line with production cost, then ignore
The stability of microbial bacteria and the trophism value of feed addictive.
In order in use, have certain adhesion, microbial bacteria feed addictive be made to exist in microbial bacteria additive
It admixes when being mixed in animal feed, can be adsorbed on animal feed, convenient for the uniformity in animal feeding and be fed into bacterium amount
Maximization, some products take pains a certain amount of binder of addition.
In order to make to raise the relative constant of animal stomach inner pH value, acid is continued up to after so that the foodstuff being fed into is entered small intestine
The effect of change, in the product often simultaneously using acidulants such as citric acid, lactic acid, hydrochloric acid, fumaric acid, but these acidulants are not only straight
It connects and locally causes acidity too strong microbial bacteria contained by additive, influence the survival of thallus, meanwhile, these acidifying substances are to cultivation
Animal also without the nutritive value directly utilized, meanwhile, there is very big irritation to the gastrointestinal tract of the cubs of livestock and poultry, young fowl,
Compatibility is using not ideal enough.
In order to make bacillus cultivated animals application in reach better application effect, especially have been reported that show it is right
Bacillus subtilis using effect is added more preferably in the animal feed formulations bacillus licheniformis such as pig, ox, sheep.
Above-mentioned deficiency can be overcome by developing one kind, can either keep stability, the trophism of institute's mycetome, be had again to thallus
There is adhesion, while having again to the filler used and providing the additive for microbe feedstuff of nutritive value to cultivated animals, is
Promote the basis of feed additive industry long-term stability development.
Summary of the invention
The purpose of the present invention is to provide a kind of new composite bacillus feed addictive, the composite bacillus feeds
Not only there is additive thallus to stablize, and the thallus time-to-live is long, plays the more effect of use in conjunction, and used complementary
Material has certain adhesiveness and adsorptivity, and thallus can be made preferably to be bonding on one with auxiliary agents, meanwhile, it is complementary
There is additive certain acidity can not only provide nutriment abundant, while its acidic materials can be protected after animal feeding
It is relative constant to hold stomach inner pH value, into small intestine after the effect of continuing up to acidification.The purpose is to provide it is a kind of have more stability and
The composite bacillus feed addictive of trophism.
A kind of composite bacillus feed addictive of the present invention, it is characterized in that by bacillus licheniformis, bacillus subtilis
It is uniformly mixed with a certain amount of seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant powder, cactus powder and fermentation egg-shell meal, obtains one kind
The thallus of new composite bacillus feed addictive, this additive is stablized, and the thallus time-to-live is long, and seabuckthorn fruit powder, Rosa roxburghii Tratt
Powder, wintercherry powder, blackcurrant powder sugar content are high, and especially cactus powder all has preferable Adhesion property, and fermentation egg-shell meal also has
There is preferable adsorptivity.Wherein sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant are the ascorbic fruits of high-content, and acidity is extremely strong, no
The nutriments such as vitamin abundant only are provided to cultivated animals, the acidic materials after feeding play promotes digestion well
Effect, to highlight the purpose of the present invention.
In order to realize the present invention, the following technical solutions are specifically adopted：
A kind of composite bacillus feed addictive, feature it be first by bacillus licheniformis, bacillus subtilis, sea-buckthorn
Freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the hair of surplus
Ferment egg-shell meal is uniformly mixed, and composite packaging is made：Bacillus licheniformis 5000 hundred million -60000 in every 1000g feed addictive
A hundred million, bacillus subtilis 5000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-drying
Powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is fermentation egg-shell meal.
Bacillus licheniformis described above is bacillus licheniformis dry powder product；The bacillus subtilis is withered grass
Bacillus dry powder product.
The bacillus licheniformis dry powder product and bacillus subtilis dry powder product, can be respectively using commercially available
Be used as every gram of feed addictive and be no less than 200 hundred million preparations containing viable bacteria concentration, the bacillus licheniformis dry powder product,
Bacillus subtilis dry powder product and formula in filler material weight proportion by thalli dry powder and final products thallus content
It determines.
The bacillus licheniformis dry powder product and bacillus subtilis dry powder product also can use existing method choosing
The culture medium accordingly optimized is selected, respectively by the culture of liquid fermentation equipment, then uses high speed centrifugation mode by the bacterium in fermentation liquid
Body is separated, and is placed in dry indoor cold wind or bacterium mud drying is made with vacuum freeze drying mode, obtain respectively
Every gram of viable bacteria concentration no less than 200 hundred million Bacillus licheniformis soma powder product and every gram of viable bacteria concentration no less than 200
Hundred million bacillus subtilis thalli dry powder product.
Sea-buckthorn freeze-dried powder described above, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder,
It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively,
The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing condition described above is
With the citric acid of 40 DEG C of water addition weight 0.1%, 15min is rinsed, and be stirred continuously, pulls out to be put into pure water and rinse 3 times；On
Stating freezing is that will be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；Above-mentioned distillation
Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；It is above-mentioned
Low-temperature grinding is the crushing that temperature of charge is in 0 DEG C or less progress, 100 mesh of grinding particle size >.
Composite packaging described above uses interior outer bag composite packaging, and inner bag uses polypropylene plastics pocket, and outer packing uses aluminium
Foil bag is filled with silica-gel desiccant between interior outer bag.
Fermentation egg-shell meal described above, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, so
After be ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the ferment of egg-shell meal weight 20%
Female bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials is adjusted to temperature
Carbonyl ammonia is carried out under the conditions of being 80-90% for 82-92 DEG C, humidity（Also referred to as Maillard reaction）Reaction 22-28 days, then, by material
Moisture content < 5%/w is dried under the conditions of being not more than 100 DEG C using electric baking case, is crushed, is sieved with 100 mesh sieve to obtain the final product.
Drying described above uses heated-air drying or far-infrared ray drying；Dry condition is 180 DEG C -300 DEG C, drying
Purpose kill the harmful substances such as microorganism and helminth first is that reaching, second is that the crisp matterization of eggshell can be made to be conducive to crush, and make powder
Egg-shell meal after broken is loose conducive to fermentation.
The carbonyl ammonia reaction, i.e. Maillard reaction, also known as " non enzymatic browning reacton ", are carbonyls（Reduced sugar
Class）And amino-compound（Amino acid and protein）Between reaction.
The above-mentioned purpose that eggshell coarse powder is used to saccharomycetes to make fermentation, first is that can effectively make eggshell sufficiently soft using fermentation process
Change, the egg-shell meal after making fermentation is loose, is easy to mix with unclassified stores；Second is that can effectively be made in eggshell using saccharomycetes to make fermentation
The ingredients such as calcium obtain effective bioconversion, form BIOLOGICAL CALCIUM, conducive to being absorbed and utilized for animal, saccharomycetes to make fermentation can make fermentation material
Material forms the mass propagation of more protein, especially saccharomycete, and it is peculiar to increase yeast institute for the aggregation for the ingredient that can have additional nutrients
The multiple nutritional components such as glucose, protein, nucleic acid, vitamin, carbohydrate, lipid-like substances, especially B race dimension life
Element such as B1、B2、B6、B12And the physiological activators such as folic acid, niacin, pantothenic acid, inositol get a promotion, these beneficial nutrients
Matter ingredient undoubtedly plays the nutritional ingredient for improving product of the present invention, improves its nutritive validity.
The further carbonyl ammonia of egg-shell meal is reacted in the present invention, first is that promoting the maturing process of eggshell, is further made wherein
Calcareous ingredient obtain sufficient bioconversion, biological calcium component is formed, second is that obtaining the protein component in material sufficiently
Bioconversion, amino acid is degraded to, third is that a large amount of aminoacid ingredients to be formed is made to be converted into the small molecules object such as small peptide and small peptide
Matter, conducive to digesting and absorbing after animal feeding, the reaction of carbonyl ammonia not only promotes the trophic function of product, after the reaction of carbonyl ammonia
Material can further increase the application that gas porosity is conducive to mix feed, while carbohydrate being made to be converted into reduced sugar, produce simultaneously
Raw fragrance matter abundant, not only improves the anti-immunity ability of product, further improves the flavor of product, increase being fond of for animal
Property.
Eggshell described above is the mixed of any one or a few in egg shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell
Close eggshell.
Saccharomycete described above（saccharomyces）For candida utili（Candida utilis）, the torrid zone it is false
Silk yeast（Candida tropicalis）, brewer's yeast（Saccharomyces cerevisiae）, brewer's yeast
（Saccharomyces cerevisiae）And fodder yeast（Candida Tropicali）In any one.
Above-mentioned saccharomycete can choose using all viable bacterias as food additives or feed addictive commercially available
Concentration is no less than 200 every gram hundred million thalli dry powder products.
Existing method preparation can also be used in above-mentioned saccharomycete, such as can accordingly be optimized according to the selection of the property of strain
Then thallus in fermentation liquid is separated with high speed centrifugation mode, is set by the culture of Liquid Culture Zymolysis Equipment by culture medium
Bacterium mud drying is made in the indoor cold air drying of drying or with freeze-drying mode, obtaining viable bacteria concentration respectively is every gram
200 hundred million thalli dry powder products.
A kind of prioritization scheme of composite bacillus feed addictive of the present invention is, first by bacillus licheniformis, withered grass
Bacillus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed,
The fermentation egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made：The bacillus licheniformis in every 1000g feed addictive
7000 hundred million -60000 hundred million, bacillus subtilis 7000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder
8-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70-150g, remaining is fermentation egg-shell meal.
The further prioritization scheme of the present invention is a kind of composite bacillus feed addictive, it is characterized in that first by ground
Clothing bacillus, bacillus subtilis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus
Freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging is made：In every 1000g feed addition
Bacillus licheniformis 9000 hundred million -60000 hundred million in agent, bacillus subtilis 9000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder
15-50g, Rosa roxburghii freeze-dried powder 12-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85-
150g, remaining is fermentation egg-shell meal.
Further optimization plan of the present invention is a kind of composite bacillus feed addictive, it is characterized in that first by
Bacillus licheniformis, bacillus subtilis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being
Slap freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging is made：Add in every 1000g feed
Add bacillus licheniformis 10000 hundred million -60000 hundred million in agent, bacillus subtilis 10000 hundred million -60000 hundred million, sea-buckthorn is frozen
Dry powder 20-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder
100-150g, remaining is fermentation egg-shell meal.
Bacillus licheniformis (Bacillus licheniformis) cellular morphology and the in the shape of a rod, Dan Sheng of arrangement, can adjust
Flora imbalance reaches therapeutic purposes, and body can be promoted to generate antibacterial substance, kill pathogenic bacteria.Resistant activity material can be generated,
And there is unique biology take oxygen mechanism, the growth and breeding of pathogenic bacteria can be inhibited.Suitable for aquaculture, feed addition and
Pharmacy.Aquatic livestock enteritis, the diseases such as gill rot can effectively be prevented；The poisonous and harmful substance in aquaculture pond can be decomposed, is purified water；
There is the activity of stronger protease, lipase, amylase, promotes nutrient degradation in feed, make aquatic product animal to feed
It is absorbed and utilized more sufficiently；The development of aquatic livestock immune organ is stimulated, immunity of organisms is enhanced.Bacillus licheniformis is suitable for
Intestinal bacilli illness disease and enteron aisle caused by bacterium reason need the cultivated animals of health care.It is fuller to poultry animal effect
Meaning, such as chicken, duck, goose.Bacillus licheniformis is mainly used for aquatic livestock at present, domestic birds and animals promote normal physiological in enteron aisle
The growth of anaerobism adjusts intestinal bacilli illness, restores function of intestinal canal；There is special efficacy to enterobacterial infection, to light-duty or heavy
Chordapsus, light-duty and medium-sized acute bacillary dysentery etc., there is obvious curative effects；Resistant activity material can be generated, and is had unique
Biology take oxygen mechanism can inhibit the growth and breeding of pathogenic bacteria.
Bacillus subtilis（Bacillus subtilis）, it is one kind of bacillus.It is generated during thalli growth
Subtilin, polymyxins, nystatin, gramicidins isoreactivity substance, these active materials are to pathogenic bacteria or endogenous
The conditioned pathogen of infection has apparent inhibiting effect.The free oxygen in enteron aisle can be consumed after bacillus subtilis is for oral administration rapidly,
Enteron aisle hypoxemia is caused, promotes to grow beneficial to anaerobic bacteria, and generate the organic acids such as lactic acid, enteron aisle pH value is reduced, inhibits indirectly
Other pathogenic bacteria growths.Can stimulate the growth and development of animal immune organ, activate T, bone-marrow-derived lymphocyte, improve immunoglobulin and
Antibody level enhances cellular immunity and humoral immune function, improves herd immunity.Bacillus subtilis thallus itself synthesis
The enzymes such as alpha-amylase, protease, lipase, cellulase play together with the intracorporal digestive enzymes of animal in alimentary canal
Effect.
Bacillus subtilis can synthesize a variety of B family vitamins such as vitamin B1, B2, B6, niacin, improve in animal body and do
Disturb the activity of element and macrophage.Bacillus subtilis is to harmful micro- life such as vibrios, Escherichia coli and baculoviral in aquatic products
Object has very strong inhibiting effect, effectively prevents aquatic livestock enteritis, the diseases such as gill rot.The function of a large amount of chitinases is secreted, it is several
Fourth matter enzyme can decompose the cell wall of disease fungus and inhibit fungal disease, decompose the poisonous and harmful substance in aquaculture pond, purified water
Matter；Residual bait, excrement, organic matter etc. in decomposing pool have the short grained effect of rubbish in very strong cleaning water；
Bacillus subtilis can improve water turbidity problem caused by harmful cyanobacteria is overflowed, and water quality becomes clear by muddy, have very strong
Function For Purifying Water, with stronger protease, lipase, amylase activity, promote feed in nutrient degradation, make water
Produce class animal being absorbed and utilized more sufficiently to feed；
Bacillus subtilis can reduce shrimp disease, can greatly improve prawn yield, thus increase economic efficiency, it is raw
Object environmental protection, stimulates the development of aquatic livestock immune organ, enhances immunity of organisms；Reduce shrimp disease, hence it is evident that raising pair
Shrimp yield purifies water to increase economic efficiency, pollution-free, noresidue.
Sea-buckthorn（Hippophae rhamnoides Linn.）, fruit nutriment rich in and biological active matter
Matter can be widely applied to many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Fructus hippophae and oil
With very high medical value, there is a cough-relieving apophlegmatic, relieving dyspepsia, promoting blood circulation to remove blood stasis effect, can treat gastric duodenal ulcer with
And indigestion etc..
Sea buckthorn fruit is full of nutrition, according to surveying and determination in its fruit containing multivitamin, fatty acid, microelement, sub- oil element,
The various amino acid of Hippophate flavone, superoxides isoreactivity substance and needed by human body.Wherein Vitamin C content is high, and every 100 grams
In fruit juice, Vitamin C content can reach 825-1100 milligrams, is 2-3 times of Kiwi berry, is known as the laudatory title of the king of vitamin C.Contain
Sugared 7.5%-10%, the 3%-5% containing acid.
Rosa roxburghii Tratt（Rosa roxbunghii）For the fruit of the perennial machaka rosa roxburghii of rosaceae also known as mountain king fruit, thorn
Certain kind of berries fruit, thatch pears etc..With clearing heat and promoting fluid, stomach strengthening and digestion promoting, relieving summer-heat and other effects.Rosa roxburghii Tratt vitamin B rich in, C, carrotene,
The ingredients such as malic acid, citric acid are usually used in deficiency of stomach-Yin, appetite stimulator, indigestion or eating accumulation, glutted full bored, diarrhea
Loose stool；Pyreticosis or hot summer weather hurt saliva, thirsty, scanty drak urine.
Mature Rosa roxburghii Tratt is fleshy hypertrophy, sweet and sour, fruit are rich in sugar, vitamin, carrotene, organic acid and more than 20 kinds of ammonia
More than base acid, 10 microelement and superoxide dismutase, especially Vitamin C content beneficial to human body are high, are to work as
It is highest in preceding fruit, it is 50 times of citrus 841.58 milligrams -3541.13 milligrams of content in every 100 grams of fresh fruits, Kiwi berry
10 times, the laudatory title with " king of vitamin C ".Fruit of Grossularia burejensis Berger has very high nutritive value and medical value, sour, puckery, flat,
Digestion-promoting spleen-invigorating restrains stopping leak, for treating having indigestion abdominal distension, dysentery, enteritis, vitamin C deficiency etc..
Wintercherry（Physali alkekengiL.）Also known as groundcherry, franchet groundcherry persistent calyx or fruit, cape gooseberry etc..Containing more in Fruit of Physalis
Kind of nutritional ingredient, wherein the content of calcium be 73.1 times of tomato, 13.8 times of carrot, ascorbic content is tomato
6.4 times, 5.4 times of carrot.With unique flavor and nutrition abundant.Fruit of Physalis aromatic flavour, delicious.Berry is rich
Containing vitamin C, beta carotene, more than 20 kinds of mineral elements and 18 kinds of amino acid, there is heat-clearing, removing toxic substances, diuresis effect is usually used in
Control heat-syndrome cough, pharyngalgia, jaundice, dysentery, in water, the treatment of furunculosis, erysipelas etc..
Blackcurrant scientific name elements of black currant（Ribes nigrum L.）Also known as currant, purple plum etc., ripening fruits are black
Small berries, it is interior to be rich in vitamin C, anthocyanidin.Blackcurrant is not only a kind of delicious flavour, fruit full of nutrition, and has
Good medicinal efficacy.Blackcurrant fruit is rich in mineral matter element, and the Zn content in mineral matter element is high, can be used as and comes for zinc
Source；Ascorbic content is high, hence it is evident that is higher than citrus, hawthorn, also contains phosphorus, magnesium, potassium, calcium, anthocyanidin, phenolic substances.Seed
Linoleic acid content is high.Ascorbic content is very rich in blackcurrant, it is often more important that is also melted into containing a large amount of antioxygen
Point, this assures ascorbic function and effect.Blackcurrant is rich in such as anthocyanidin, vitamin C, flavones, Quercetin, red bayberry
The substances such as alcohol, phenolic acid, catechin and blackcurrant polysaccharide, these are all the bioactive substances with good anti-oxidation function.
Cactus（Scientific name：Opuntia stricta(Haw.)Haw.var.dillenii(Ker-Gawl.) Benson）,
It is a kind of plant of Opuntia.Alias celestial being palm, kwan-yin palm etc..Cactus is the meat shrub that grows thickly, the wide ovum of top branch
Shape, obovate elliptic or subcircular；Flower spoke shape, holder obovate.It is used as medicine with complete stool.The four seasons can adopt.
Cactus is cool in nature, sour, nontoxic.Have the promoting flow of qi and blood circulation, heat-clearing toxin-expelling functions.Mainly have clearing heat and detoxicating, eliminating stasis to subdue swelling,
The functions such as the promoting flow of qi and blood circulation, benefiting stomach and stopping pain.Cactus contains amino acid and various trace elements in needed by human 8, and embraces green
The preciousness ingredient such as lotus, Kok Beattie cents, cholla stem, starches through cactus crude drug and analyzes, contain pectin, polysaccharide and glue exudate.From herb point
From extracting to obtain freidelin ketone（friedelin）, -3 α -ol of freidelin（friedelan-3-α-ol）, taraxerone
（taraxerone）And taraxerol（taraxerol）.
Eggshell, quantity is biggish in China's poultry farming chicken, duck, goose and quail etc., using egg, duck's egg,
As in food processing process, the useless eggshell enormous amount generated is provided according to existing disclosed report for goose egg and quail egg
Material display, the eggshell total amount that a medium-sized city is monthly discarded reach 60-100 tons, and account for egg size by Eggshell weight 11% calculates,
The eggshell that about 1,500,000 tons of the daily output in China, not only pollutes environment, but also waste of resource in this way.Discarded eggshell is integrated
It utilizes, resource utilization can not only be improved and avoids environmental pollution, and be greatly improved the economic benefit and social benefit of enterprise.
Wherein egg shell is in China's yield maximum, and egg shell accounts for the 10-12% in entire egg, by under film, shell on shell
Film and eggshell three parts composition, eggshell is a kind of calcareous hard shell, is located on shell between film and shell lower film.In the change of egg shell
In studying point, minerals account for 95%, and protein 3.3%, moisture 1%, fat 0.03%, remaining is carbohydrate and other have
Machine substance（It certainly, does not include a large amount of remaining egg white in eggshell in the above ingredient）.Eggshell mineral ingredient is mainly
Calcium carbonate accounts for 94% or so, remaining is magnesium carbonate and calcium phosphate, and calcium, phosphorus content account for 34.9% and of eggshell dry matter respectively
0.2%.In eggshell, seven kinds of vitamins and various trace elements and some important enzymes, peptide matters can be measured, he
Although content seldom, be all the substance needed in human body or animal body.
In the prior art, it is replenished the calcium with egg-shell meal, has the product of the grinds after sterilizing by eggshell in people's product, also have
The application of organic calcium is prepared into using primary or secondary clacining method.It is at present most widely in drug, food and nutriment
In preparation, micron order, submicron order and nano level superfine powder are mostly used, is added to various food, nutrition kind, has reached very
Good effect.Application of the eggshell in China's animal-breeding is taken mostly by eggshell cleaning, drying, and milling is added into feed
The method of feeding, since grinding particle size is larger, being absorbed and utilized for animal is poor, can't reach good application effect.
The present invention is first to be ground into coarse powder by after eggshell cleaning, high temperature drying, fermented using saccharomycete, then again
Carbonyl ammonia reaction treatment is carried out, then dried, crush and be prepared into biological calcium product, the program not only improves what eggshell utilized
Nutrition, while the main composition-calcium carbonate in eggshell being made to obtain biochemical reaction, the biological calcium carbonate for being more conducive to absorb is formed,
The reaction of carbonyl ammonia makes its protein component be further converted to amino acid again, makes the protein, amino acid converting for small peptide of macromolecular
Or the small-molecule substances such as small peptide, the beneficial effect for reaching collection eutrophy and being absorbed and utilized.
The beneficial effects of the invention are as follows：
（1）Product of the present invention can highlight the viable bacteria content of bacillus licheniformis and bacillus subtilis, be obviously improved gemma bar
The more preferably effect of bacterium use in conjunction significantly improves survival rate of the thallus in closed composite packaging, through batches of products in room temperature item
The average viability of reserved sample observing 12 months under part, bacillus licheniformis and bacillus subtilis is 93.2%, and reference substance（It is right
Filler according to product is wheat bran）In bacillus licheniformis and bacillus subtilis survival rate be 72%；
（2）Pass through sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder and lichens bud
Spore bacillus and bacillus subtilis mix, due to the viscosity in the colloidal substance that these dry powder formulations are rich in, especially cactus
Substance can preferably increase the adhesive effect between additive and feed, and improve the utilization rate of additive；
（3）By sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder high-content amino acid, vitamin, mineral element, more
Sugar.The nutritional ingredients such as the vitamin C of organic acid, especially high-content and various bioactivators, to raising animal immune energy
Power is simultaneously obviously improved meat, Egg Quality, the acidic materials in especially above-mentioned dry powder, utilization, promotion to contained complex microorganism
Animal digestion all has significant effect；
（4）Due to using Freeze Drying Technique means, enable sea-buckthorn obtained, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder
Its active constituent is maximumlly remained, has distinctive sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the smell of cactus application and taste
Taste, free from extraneous odour add the preference of animal.
（5）Using egg-shell meal first through saccharomycetes to make fermentation, carbonyl ammonia is then used（Maillard reaction）Reaction is prepared into rich in life
The fermentation egg-shell meal of object calcium, not only makes the calcium carbonate in eggshell obtain bioconversion, obtains the BIOLOGICAL CALCIUM for being conducive to animal absorption,
And further converted to a large amount of protein, amino acid, carbohydrate is formed after saccharomycetes to make fermentation, make amino
Acid, small peptide or small peptide small-molecule substance get a promotion, and more conducively animal is absorbed and utilized, and pass through what conversion carbohydrate was formed
Inverted sugar and fragrance matter further improve the flavor and taste of product, increase animal to the preference of product.
（6）To the preparation of sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder in the present invention, do not remove the peel, deseeding step
Suddenly, it is therefore an objective to by being crushed after freeze-drying, so that its skin and seed is worn into fine powder, make anthocyanidin, oil in its skin and seed
Class, organic acid, bud fragrance substance are retained, and the nutritive value of its freeze-dried powder is promoted.
（7）The present invention is lyophilized using sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus
Powder, matrix of the fermentation egg-shell meal as additive contain constants such as a large amount of calcium, phosphorus, potassium, sodium, magnesium, zinc and micro in matrix
Element, vitamin C still rich in, anthocyanidin, beta carotene, malic acid, citric acid, protein, amino acid, nucleic acid, Portugal
Grape sugar, lipoidis, carbohydrate, B family vitamin such as B1、B2、B6、B12And the physiological activity such as folic acid, niacin, pantothenic acid, inositol
Substance, not only improves the nutritive value of composite bacillus additive, while promoting the metabolism of animal, improves the anti-of body
Sick ability promotes growth of animal, finally improves the economic benefit of its aquaculture.
（8）Product of the present invention uses interior outer bag composite packaging, and interior outer bag interlayer is filled with silica-gel desiccant, and product does not return
Tide, no agglomeration, product is loose, good fluidity, can preferably keep exterior quality and inherent quality.
Operation to not having its technical process stated in detail in the present invention is in the common sense of those skilled in the art
Operation.With regard to the purchase and storage of heretofore described sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant fresh fruit and fresh cactus, the present invention
The construction of cooperation thousand Yao Tang traditional Chinese medical science Co., Ltd of research institute of unit Jiangsu has 20,000 tons of freezer facility, is to have to storage fresh fruit material
It ensures enough.
Specific embodiment
Embodiment 1
The preparation of sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder：
Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the cactus for taking fresh mature respectively remove carpopodium, the impurity such as rotten fruit, with containing heavy
40 DEG C of aqueous solutions of 0.1% citric acid are measured, 15min is rinsed under constant stirring and pulls out, be put into pure water and rinse 3 times, pull out
It is drained；It will be moved into refrigerator after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively, be cooled to -45 DEG C rapidly
Below；Respectively by the sea-buckthorn freezed, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus move into lyophilization machine in, sublimation temperature control-
22--32 DEG C, vacuum degree 120-150Pa makes dry materials to moisture < 6%（w）, the time about needs 18 hours, is put into after taking-up
In freezing crusher, control temperature of charge is no more than 0 DEG C, after being crushed to 100 mesh of fineness >, respectively obtains sea-buckthorn freeze-dried powder, thorn
Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are fitted into polybag and seal, and are stored in 0 DEG C or less
It is spare in freezer.
Embodiment 2
Using the culture medium accordingly optimized, candida utili, torrid zone vacation are individually cultivated using Liquid Culture Zymolysis Equipment
Silk yeast, brewer's yeast, brewer's yeast and fodder yeast, are then separated the thallus in fermentation liquid with supercentrifuge,
It is with vacuum freeze drying mode that bacterium mud is dry, respectively obtain candida utili dry powder that viable bacteria content is 200 hundred million/grams,
Candida tropicalis dry powder that viable bacteria content is 200 hundred million/grams, the brewer's yeast dry powder that viable bacteria content is 200 hundred million/grams, work
The feed yeast powder that the brewer's yeast dry powder and viable bacteria content that bacterial content is 200 hundred million/grams are 200 hundred million/grams.
Embodiment 3
The preparation of fermentation egg-shell meal：
One or more of mixing eggshells of egg shell or Ovum Anas domestica putamina, goose egg-shell, quail eggshell are cleaned up, moisture is dried, sets
Enter in baking oven, under the conditions of 240 DEG C after heated-air drying 1.5 hours, taking-up is let cool, and is ground into 45 mesh powder；It is public to weigh egg-shell meal 80
Jin, suitable pure water, which is added, makes into material moisture 50%（W）, add the feed yeast powder 20 that embodiment 2 obtains
Kilogram, rear sabot is mixed well, fermenting cellar is placed in, is fermented 60-72 hours under the conditions of temperature is 31-33 DEG C, to fermentation ends
Afterwards, fermentation materials are adjusted to temperature is 82 DEG C -92 DEG C, under the conditions of temperature is 80-90%, carries out carbonyl ammonia and reacts 22-28 days, so
Material is dried to moisture content < 5% under the conditions of being not more than 100 DEG C using electric baking case afterwards（W）, utilize omnipotent crushing
Machine is crushed, and crosses 100 sieves to get fermentation egg-shell meal.
With the preparation method of the fermentation egg-shell meal of embodiment 3, it is dry that candida utili obtained in embodiment 2 can be respectively adopted
Powder, candida tropicalis dry powder, brewer's yeast dry powder or brewer's yeast dry powder carry out preparation fermentation egg-shell meal, other process conditions
Implemented according to eggshell powder preparation technique parameter of fermenting in the claim of this application 1-4.
Embodiment 4
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 5000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 60000 hundred million, sea-buckthorn freeze-dried powder 5g, Rosa roxburghii freeze-dried powder 50g, wintercherry freeze-dried powder 3g, blackcurrant freeze-dried powder 3g, cactus are frozen
Dry powder 50g, remaining is fermentation egg-shell meal；Specifically production method is：Weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii Tratt respectively
Freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mix, then by bacillus licheniformis dry powder and withered grass bud
Spore bacillus dry powder is uniformly admixed, then add surplus ferment as made from embodiment 3 egg-shell meal be sufficiently mixed it is uniform after, answered
Close packaging；Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, adds a few packet silica dehydrators between interior outer bag
Agent, to prevent water suction.
Embodiment 5
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 60000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 6000 hundred million, sea-buckthorn freeze-dried powder 45g, Rosa roxburghii freeze-dried powder 10g, wintercherry freeze-dried powder 5g, blackcurrant freeze-dried powder 5g, cactus are frozen
Dry powder 100g, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 6
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 10000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 10000 hundred million, sea-buckthorn freeze-dried powder 30g, Rosa roxburghii freeze-dried powder 15g, wintercherry freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, celestial being
Freeze-dried powder 115g is slapped, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 7
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 60000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 10000 hundred million, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 15g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 12g, celestial being
Freeze-dried powder 100g is slapped, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 8
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 60000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 60000 hundred million, sea-buckthorn freeze-dried powder 10g, Rosa roxburghii freeze-dried powder 30g, wintercherry freeze-dried powder 20g, blackcurrant freeze-dried powder 20g, celestial being
Freeze-dried powder 110g is slapped, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 9
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 9000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 50000 hundred million, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, celestial being
Freeze-dried powder 120g is slapped, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 10
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 50000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 40000 hundred million, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 30g, wintercherry freeze-dried powder 30g, blackcurrant freeze-dried powder 30g, celestial being
Freeze-dried powder 95g is slapped, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 11
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 45000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 45000 hundred million, sea-buckthorn freeze-dried powder 40g, Rosa roxburghii freeze-dried powder 40g, wintercherry freeze-dried powder 25g, blackcurrant freeze-dried powder 25g, celestial being
Freeze-dried powder 115g is slapped, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 12
A kind of composite bacillus feed addictive is by bacillus licheniformis dry powder, bacillus subtilis dry powder, sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus
Shell powder is uniformly mixed, and composite packaging is made：Bacillus licheniformis 55000 hundred million, withered grass gemma in every 1000g feed addictive
Bacillus 55000 hundred million, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, wintercherry freeze-dried powder 20g, blackcurrant freeze-dried powder 20g, celestial being
Freeze-dried powder 100g is slapped, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Claims (10)
1. a kind of composite bacillus feed addictive, it is characterized in that first by bacillus licheniformis, bacillus subtilis, sea-buckthorn
Freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the hair of surplus
Ferment egg-shell meal is uniformly mixed, and composite packaging is made：Bacillus licheniformis 5000 hundred million -60000 in every 1000g feed addictive
A hundred million, bacillus subtilis 5000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-drying
Powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is fermentation egg-shell meal；The sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are made, technical process by following methods
To take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus to remove carpopodium, the impurity such as rotten fruit, rinsing, freezing, distillation respectively
Dry, low-temperature grinding, is not removed the peel, deseeding step；Rinsing condition described above is the lemon with 40 DEG C of water addition weight 0.1%
Lemon acid, rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times；Above-mentioned freezing be respectively by sea-buckthorn, Rosa roxburghii Tratt,
It is cooled to -45 DEG C or less rapidly after wintercherry, blackcurrant, cactus sabot；The process conditions of above-mentioned lyophilization are temperature-22-
It is -32 DEG C, vacuum degree 120-150Pa, dry until moisture is less than 6%/w；The fermentation egg-shell meal is that eggshell cleaning is dry
Only, moisture is dried, is dried 1-2 hours in advance, is then comminuted into 40-50 mesh powder, suitable water is added makes into water content and exist
45-55%/w, the saccharomycete for adding egg-shell meal weight 20% are mixed thoroughly, and fermentation 60-72 is small under the conditions of temperature is 31 DEG C -33 DEG C
When, after fermentation, fermentation materials are adjusted to temperature and is 82-92 DEG C, carries out the reaction of carbonyl ammonia under the conditions of humidity is 80-90%
22-28 days, then, material is dried to moisture content < 5%/w under the conditions of being not more than 100 DEG C using electric baking case, is passed through
It crushes, sieves with 100 mesh sieve to obtain the final product.
2. a kind of composite bacillus feed addictive, it is characterized in that first by bacillus licheniformis, bacillus subtilis, sea-buckthorn
Freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the hair of surplus
Ferment egg-shell meal is uniformly mixed, and composite packaging is made：Bacillus licheniformis 7000 hundred million -60000 in every 1000g feed addictive
A hundred million, bacillus subtilis 7000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 8-50g, wintercherry freeze-drying
Powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70-150g, remaining is fermentation egg-shell meal；The sea-buckthorn freeze-drying
Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are made, technical process by following methods
To take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus to remove carpopodium, the impurity such as rotten fruit, rinsing, freezing, distillation respectively
Dry, low-temperature grinding, is not removed the peel, deseeding step；Rinsing condition described above is the lemon with 40 DEG C of water addition weight 0.1%
Lemon acid, rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times；Above-mentioned freezing be respectively by sea-buckthorn, Rosa roxburghii Tratt,
It is cooled to -45 DEG C or less rapidly after wintercherry, blackcurrant, cactus sabot；The process conditions of above-mentioned lyophilization are temperature-22-
It is -32 DEG C, vacuum degree 120-150Pa, dry until moisture is less than 6%/w；The fermentation egg-shell meal is that eggshell cleaning is dry
Only, moisture is dried, is dried 1-2 hours in advance, is then comminuted into 40-50 mesh powder, suitable water is added makes into water content and exist
45-55%/w, the saccharomycete for adding egg-shell meal weight 20% are mixed thoroughly, and fermentation 60-72 is small under the conditions of temperature is 31 DEG C -33 DEG C
When, after fermentation, fermentation materials are adjusted to temperature and is 82-92 DEG C, carries out the reaction of carbonyl ammonia under the conditions of humidity is 80-90%
22-28 days, then, material is dried to moisture content < 5%/w under the conditions of being not more than 100 DEG C using electric baking case, is passed through
It crushes, sieves with 100 mesh sieve to obtain the final product.
3. a kind of composite bacillus feed addictive, it is characterized in that first by bacillus licheniformis, bacillus subtilis, sea-buckthorn
Freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the hair of surplus
Ferment egg-shell meal is uniformly mixed, and composite packaging is made：Bacillus licheniformis 9000 hundred million -60000 in every 1000g feed addictive
A hundred million, bacillus subtilis 9000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 12-50g, wintercherry are frozen
Dry powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85-150g, remaining is fermentation egg-shell meal；The sea-buckthorn is frozen
Dry powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are made by following methods, technique mistake
Journey is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium, the impurity such as rotten fruit respectively, and rinsing, rises at freezing
Magnificent drying, low-temperature grinding are not removed the peel, deseeding step；Rinsing condition described above adds weight 0.1% with 40 DEG C of water
Citric acid rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times；Above-mentioned freezing is respectively by sea-buckthorn, thorn
It is cooled to -45 DEG C or less rapidly after pears, wintercherry, blackcurrant, cactus sabot；The process conditions of above-mentioned lyophilization are temperature-
It is 22-- 32 DEG C, vacuum degree 120-150Pa, dry until moisture is less than 6%/w；The fermentation egg-shell meal is by eggshell
It cleans up, dries moisture, be dried 1-2 hours in advance, be then comminuted into 40-50 mesh powder, suitable water is added makes into and contain
Water is mixed thoroughly in 45-55%/w, the saccharomycete for adding egg-shell meal weight 20%, is fermented under the conditions of temperature is 31 DEG C -33 DEG C
60-72 hours, after fermentation, fermentation materials is adjusted to temperature and is 82-92 DEG C, carries out carbonyl under the conditions of humidity is 80-90%
Ammonia reacts 22-28 days, then, material is dried to moisture content < under the conditions of being not more than 100 DEG C using electric baking case
5%/w is crushed, is sieved with 100 mesh sieve to obtain the final product.
4. a kind of composite bacillus feed addictive, it is characterized in that first by bacillus licheniformis, bacillus subtilis, sea-buckthorn
Freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the hair of surplus
Ferment egg-shell meal is uniformly mixed, and composite packaging is made：The bacillus licheniformis 10000 hundred million-in every 1000g feed addictive
60000 hundred million, bacillus subtilis 10000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 15-50g,
Wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 100-150g, remaining is fermentation egg-shell meal；Institute
Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, the cactus freeze-dried powder stated, by following methods system
, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium, the impurity such as rotten fruit, drift respectively
It washes, freeze, lyophilization, low-temperature grinding, not removing the peel, deseeding step；Rinsing condition described above is added with 40 DEG C of water
The citric acid of weight 0.1% rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times；Above-mentioned freezing is difference
- 45 DEG C or less will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot；The technique item of above-mentioned lyophilization
Part is temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；The fermentation egg-shell meal,
It is to clean up eggshell, dries moisture, be dried 1-2 hours in advance, be then comminuted into 40-50 mesh powder, is added suitable
Water makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed thoroughly, is 31 DEG C of -33 DEG C of items in temperature
It ferments 60-72 hours under part, after fermentation, fermentation materials is adjusted to temperature is 82-92 DEG C, humidity is 80-90% condition
Lower progress carbonyl ammonia reacts 22-28 days, then, material is dried to moisture under the conditions of being not more than 100 DEG C using electric baking case
Content < 5%/w, is crushed, is sieved with 100 mesh sieve to obtain the final product.
5. a kind of composite bacillus feed addictive as described in claim 1-4, which is characterized in that the lichens gemma bar
Bacterium and bacillus subtilis are no less than 200 hundred million thalli dry powder products for every gram containing viable bacteria concentration.
6. a kind of composite bacillus feed addictive as described in claim 1-4, which is characterized in that the low-temperature grinding is
Temperature of charge is in 0 DEG C or less and is crushed.
7. a kind of composite bacillus feed addictive as described in claim 1-4, which is characterized in that the composite packaging is adopted
With interior outer bag composite packaging, inner bag uses polypropylene plastics pocket, and outer packing uses aluminium foil bag, it is dry that silica gel is filled between interior outer bag
Drying prescription pouch.
8. a kind of composite bacillus feed addictive as described in claim 1-4, which is characterized in that the eggshell is egg
Any one or a few mixing eggshell in shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell.
9. a kind of composite bacillus feed addictive as described in claim 1-4, which is characterized in that described is dry using heat
Air-dry dry or far-infrared ray drying；The drying condition is 180 DEG C -300 DEG C.
10. a kind of composite bacillus feed addictive as described in claim 1-4, which is characterized in that the saccharomycete is
Any one in candida utili, candida tropicalis, brewer's yeast, brewer's yeast and fodder yeast.
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| CN201810511732.5A CN108850438A (en) | 2018-05-25 | 2018-05-25 | A kind of composite bacillus feed addictive |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113349285A (en) * | 2021-04-28 | 2021-09-07 | 天津纳尔生物科技有限公司 | Microbial fermentation feed additive and production method thereof |
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2018
- 2018-05-25 CN CN201810511732.5A patent/CN108850438A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113349285A (en) * | 2021-04-28 | 2021-09-07 | 天津纳尔生物科技有限公司 | Microbial fermentation feed additive and production method thereof |
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