CN108850473A - A kind of bacillus laterosporus feed addictive - Google Patents
A kind of bacillus laterosporus feed addictive Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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Abstract
A kind of bacillus laterosporus feed addictive of the present invention, it is characterized in that being uniformly mixed by bacillus laterosporus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, the fermentation egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made：Bacillus laterosporus 10000 hundred million -120000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is fermentation egg-shell meal.The present invention is single type bacillus laterosporus feed addictive, and clear containing viable count, has specific application and Quantitatively Selecting conducive to user.The present invention is using sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder and fermentation egg-shell meal as the matrix of additive, not only improve the nutritive value of bacillus laterosporus additive, promote the metabolism of animal simultaneously, improve the disease resistance of body, promote growth of animal, finally improves the economic benefit of its aquaculture.
Description
Technical field
The invention belongs to feed additive fields, are related to a kind of microbial bacteria feed addictive for cultivated animals.Tool
Body is related to a kind of bacillus laterosporus feed addictive.
Background technique
Bacillus mainly takes oxygen by force by biology in the gastrointestinal tract and maintains intestinal eubiosis, it is colonized in short-term in enteron aisle
Afterwards, a large amount of oxygen can be consumed and maintain enteron aisle anaerobic environment, field planting power of the enhancing enteron aisle to anaerobic bacteria.In addition, bacillus
It can be monosaccharide by Starch Conversion in enteron aisle, then convert lactic acid for these monosaccharide by strains other in enteron aisle, reduce enteron aisle
PH, thus play the role of inhibit pathogen.
Probiotic composition made of bacillus and other microorganisms has a significant difference, bacillus can condition not
Gemma is formed in the case where benefit, will be protected self, and resurrection rate is high.Raw spore form exists within such product, have compared with
More advantages：1. acidproof, salt tolerant, high temperature resistant（100℃）And anti-extrusion, thus pelletize, in the processing of feed by animal gastric acid
Stability with higher during environment etc.；2. the activity with very strong protease, lipase, amylase, to vegetalitas
Carbohydrate has stronger degradation capability, and the compound of certain complexity in energy degrading plant feed, generating has antagonism
The polypeptides matter of pathogenic entero becteria ability.This bacteroid has drop to the carbohydrate of many complexity of plant component part
Solution ability, the substance that can be degraded have triglycerides, protein, starch, xylan, β-Isosorbide-5-Nitrae-galactan, pectin, carboxymethyl
Cellulose, polygalacturonase, araban, glucose, p-nitrophenyl-glucopyranoside, p-nitrophenyl-Arab
Furanoside, lichenin and p-nitrophenyl-furyl xylose etc..
Bacillus laterosporus（Also known as Brevibacillus laterosporus）It is that Minister Agriculture of China announces No. 2045 announcement《Feed adds
Add agent catalogue（2013）》, it is allowed for broiler chicken, meat duck, pig, shrimp, the agent that current bacillus laterosporus is used as additive
Type has aqua and pulvis two major classes, and aqueous solution dosage form is raw because the nutritional substance in thallus aqua easy to use continues to be metabolized
Long fermentation, therefore, the thallus of this dosage form plays pendulum, and storage life is shorter.Powder formulation is then the thallus benefit that will be lived
With drying means such as low temperature hot wind, freezings, thallus or gemma are dehydrated at a slow speed, thallus or gemma is made to be in stable drying
Dormant state recycles substance that can be feeding to be uniformly mixed as filler and relatively stable powder-type biological feedstuff is made
Additive.But with regard to current manufacturing enterprise in view of raw material be easy to get and processing cost, often select rice bran, wheat bran, corn flour, jade
One or more of rice core powder, peanut shell powder, conch meal, vermiculite power, mountain flour, calcium carbonate, medical stone, zeolite powder mixture,
Some also uses plant straw powder or grass meal as filler material.The considerations of these are in line with production cost, then have ignored microbial bacteria
Stability and feed addictive trophism value.
In order in use, have certain adhesion, microbial bacteria feed addictive be made to exist in microbial bacteria additive
It admixes when being mixed in animal feed, can be adsorbed on animal feed, convenient for the uniformity in animal feeding and be fed into bacterium amount
Maximization, some products take pains a certain amount of binder of addition.
In order to make to raise the relative constant of animal stomach inner pH value, acid is continued up to after so that the foodstuff being fed into is entered small intestine
The effect of change, in the product often simultaneously using acidulants such as citric acid, lactic acid, hydrochloric acid, fumaric acid, but these acidulants are not only straight
It connects and locally causes acidity too strong microbial bacteria contained by additive, influence the survival of thallus, meanwhile, these acidifying substances are to cultivation
Animal also without the nutritive value directly utilized, meanwhile, there is very big irritation to the gastrointestinal tract of the cubs of livestock and poultry, young fowl,
Compatibility is using not ideal enough.
Above-mentioned deficiency can be overcome by developing one kind, can either keep stability, the trophism of institute's mycetome, be had again to thallus
There is adhesion, while having again to the filler used and providing the additive for microbe feedstuff of nutritive value to cultivated animals, is
Promote the basis of feed additive industry long-term stability development.
Summary of the invention
The purpose of the present invention is to provide a kind of new single type bacillus laterosporus feed addictive, the side spore gemma bars
Not only there is bacterium feed addictive thallus to stablize, and the thallus time-to-live is long, and used auxiliary agents have certain glue
Attached property and adsorptivity can make thallus preferably be bonding on one with auxiliary agents, meanwhile, complementary additive has certain
Acidity can not only provide nutriment abundant after animal feeding, while to be able to maintain stomach inner pH value relatively permanent for its acidic materials
It is fixed, into small intestine after the effect of continuing up to acidification.The purpose is to provide the side spore gemma of a kind of more stability and trophism
Bacillus feed addictive.
A kind of bacillus laterosporus feed addictive of the present invention, it is characterized in that by bacillus laterosporus and a certain amount of sea-buckthorn
Powder, Ribes burejense powder, wintercherry powder, blackcurrant powder, cactus powder and fermentation egg-shell meal uniformly mix, and obtain a kind of new side spore gemma bar
Bacterium feed addictive, the thallus of this additive are stablized, and the thallus time-to-live is long, and seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, it is black plus
Logical sequence powder sugar content is high, and especially cactus powder all has preferable Adhesion property, and fermentation egg-shell meal also has preferable adsorptivity.
Wherein sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant are the ascorbic fruits of high-content, and acidity is extremely strong, not only mentions to cultivated animals
For nutriments such as vitamins abundant, the acidic materials after feeding play the effect for promoting digestion well, thus prominent
The purpose of the present invention is shown.
In order to realize the present invention, the following technical solutions are specifically adopted：
A kind of bacillus laterosporus feed addictive, feature it be first by bacillus laterosporus, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt freeze
Dry powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus
Uniformly, composite packaging is made：Bacillus laterosporus 10000 hundred million -120000 hundred million, sea-buckthorn in every 1000g feed addictive
Freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-
150g, remaining is fermentation egg-shell meal.
Bacillus laterosporus described above is bacillus laterosporus dry powder product.
The bacillus laterosporus dry powder product can be used and commercially available be used as every gram of feed addictive containing viable bacteria
Concentration is no less than 200 hundred million preparations, the weight proportion of the bacillus laterosporus dry powder product and the filler material in formula
It is determined by the thallus content of thalli dry powder and final products.
The bacillus laterosporus dry powder product also can use the culture medium that existing method selection accordingly optimizes and pass through
Then liquid fermentation equipment culture is separated the thallus in fermentation liquid with high speed centrifugation mode, it is indoor cold to be placed in drying
Bacterium mud drying is made, every gram of viable bacteria concentration no less than 200 hundred million thallus of acquisition with vacuum freeze drying mode for wind
Dry powder product.
Sea-buckthorn freeze-dried powder described above, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder,
It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively,
The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing condition described above is
With the citric acid of 40 DEG C of water addition weight 0.1%, 15min is rinsed, and be stirred continuously, pulls out to be put into pure water and rinse 3 times；On
Stating freezing is that will be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；Above-mentioned distillation
Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；It is above-mentioned
Low-temperature grinding is the crushing that temperature of charge is in 0 DEG C or less progress, 100 mesh of grinding particle size >.
Composite packaging described above uses interior outer bag composite packaging, and inner bag uses polypropylene plastics pocket, and outer packing uses aluminium
Foil bag is filled with silica-gel desiccant pouch between interior outer bag.
Fermentation egg-shell meal described above, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, so
After be ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the ferment of egg-shell meal weight 20%
Female bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials is adjusted to temperature
Carbonyl ammonia is carried out under the conditions of being 80-90% for 82-92 DEG C, humidity（Also referred to as Maillard reaction）Reaction 22-28 days, then, by material
Moisture content < 5%/w is dried under the conditions of being not more than 100 DEG C using electric baking case, is crushed, is sieved with 100 mesh sieve to obtain the final product.
Drying described above uses heated-air drying or far-infrared ray drying；Dry condition is 180 DEG C -300 DEG C, drying
Purpose kill the harmful substances such as microorganism and helminth first is that reaching, second is that the crisp matterization of eggshell can be made to be conducive to crush, and make powder
Egg-shell meal after broken is loose conducive to fermentation.
The carbonyl ammonia reaction, i.e. Maillard reaction, also known as " non enzymatic browning reacton ", are carbonyls（Reduced sugar
Class）And amino-compound（Amino acid and protein）Between reaction.
The above-mentioned purpose that eggshell coarse powder is used to saccharomycetes to make fermentation, first is that can effectively make eggshell sufficiently soft using fermentation process
Change, the egg-shell meal after making fermentation is loose, is easy to mix with unclassified stores；Second is that can effectively be made in eggshell using saccharomycetes to make fermentation
The ingredients such as calcium obtain effective bioconversion, form BIOLOGICAL CALCIUM, conducive to being absorbed and utilized for animal, saccharomycetes to make fermentation can make fermentation material
Material forms the mass propagation of more protein, especially saccharomycete, and it is peculiar to increase yeast institute for the aggregation for the ingredient that can have additional nutrients
The multiple nutritional components such as glucose, protein, nucleic acid, vitamin, carbohydrate, lipid-like substances, especially B race dimension life
Element such as B1、B2、B6、B12And the physiological activators such as folic acid, niacin, pantothenic acid, inositol get a promotion, these beneficial nutrients
Matter ingredient undoubtedly plays the nutritional ingredient for improving product of the present invention, improves its nutritive validity.
The further carbonyl ammonia of egg-shell meal is reacted in the present invention, first is that promoting the maturing process of eggshell, is further made wherein
Calcareous ingredient obtain sufficient bioconversion, biological calcium component is formed, second is that obtaining the protein component in material sufficiently
Bioconversion, amino acid is degraded to, third is that a large amount of aminoacid ingredients to be formed is made to be converted into the small molecules object such as small peptide and small peptide
Matter, conducive to digesting and absorbing after animal feeding, the reaction of carbonyl ammonia not only promotes the trophic function of product, after the reaction of carbonyl ammonia
Material can further increase the application that gas porosity is conducive to mix feed, while carbohydrate being made to be converted into reduced sugar, produce simultaneously
Raw fragrance matter abundant, not only improves the anti-immunity ability of product, further improves the flavor of product, increase being fond of for animal
Property.
Eggshell described above is the mixed of any one or a few in egg shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell
Close eggshell.
Saccharomycete described above（saccharomyces）For candida utili（Candida utilis）, the torrid zone it is false
Silk yeast（Candida tropicalis）, brewer's yeast（Saccharomyces cerevisiae）, brewer's yeast
（Saccharomyces cerevisiae）And fodder yeast（Candida Tropicali）In any one.
Above-mentioned saccharomycete can choose using all viable bacterias as food additives or feed addictive commercially available
Concentration is no less than 200 every gram hundred million thalli dry powder products.
Existing method preparation can also be used in above-mentioned saccharomycete, such as can accordingly be optimized according to the selection of the property of strain
Then thallus in fermentation liquid is separated with high speed centrifugation mode, is set by the culture of Liquid Culture Zymolysis Equipment by culture medium
Bacterium mud drying is made in the indoor cold air drying of drying or with freeze-drying mode, obtaining viable bacteria concentration respectively is every gram
200 hundred million thalli dry powder products.
A kind of prioritization scheme of bacillus laterosporus feed addictive of the present invention is, first by bacillus laterosporus, sea-buckthorn
Freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the hair of surplus
Ferment egg-shell meal is uniformly mixed, and composite packaging is made：The bacillus laterosporus 30000 hundred million-in every 1000g feed addictive
120000 hundred million, sea-buckthorn freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 8-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-
30g, cactus freeze-dried powder 70-150g, remaining is fermentation egg-shell meal.
The further prioritization scheme of the present invention is a kind of bacillus laterosporus feed addictive, it is characterized in that first by side
Spore bacillus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, the mixing of cactus freeze-dried powder are equal
It is even, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging is made：The side spore gemma in every 1000g feed addictive
It is bacillus 50000 hundred million -120000 hundred million, sea-buckthorn freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 12-50g, wintercherry freeze-dried powder 8-30g, black
Gallon freeze-dried powder 8-30g, cactus freeze-dried powder 85-150g, remaining is fermentation egg-shell meal.
Further optimization plan of the present invention is a kind of bacillus laterosporus feed addictive, it is characterized in that first by
Bacillus laterosporus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, the mixing of cactus freeze-dried powder are equal
It is even, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging is made：The side spore gemma in every 1000g feed addictive
Bacillus 80000 hundred million -120000 hundred million, sea-buckthorn freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 10-30g,
Blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 100-150g, remaining is fermentation egg-shell meal.
Bacillus laterosporus also known as Brevibacillus laterosporus（Brevibacillus laterosporu）, form spy
Sign：In period of delay, bacillus brevis is Gram-negative, is Gram-positive in logarithmic growth phase, thallus is in
Elongated is rod-shaped, can also be observed that the gemma of ellipse once in a while.Studying through scholar confirms, bacillus laterosporus has nematicide
Ability.Gedek thinks, contains 106CFU in every gram of feed（Colony Forming Unit）The spore of bacillus can make in piglet excrement
Coliform count than control group reduce 35%.Lonely bacterium, Escherichia coli and the rod-shaped disease that bacillus laterosporus cultivates in aquatic products
The harmful bacterias such as poison have very strong inhibiting effect, and have positive effect to water body purification.Bacillus laterosporus has secretion a large amount of
The function of chitinase, chitinase can decompose the cell wall of disease fungus and inhibit fungal disease.Vibrios is that shrimps is made to cause a disease
Common multiple harmful bacteria, experiments prove that, bacillus laterosporus is obvious to the inhibiting effect of vibrios, and can be in decomposing pool
Residual bait, excrement, organic matter etc..When harmful cyanobacteria is overflowed in pond, the water surface is covered with green, when transparency reduces, by side
Spore bacillus is sprinkled into pond, and one to two day, transparency increased, and water quality becomes clear by muddy, and transparency is held time length, has very strong
Function For Purifying Water, be very suitable for the cultivation of aquatic livestock.It is used in the cultivation of prawn, it is possible to reduce shrimp disease hair
Raw, saving frequently starts the aerator electricity charge, prawn yield can be greatly improved, to increase economic efficiency.
Sea-buckthorn（Hippophae rhamnoides Linn.）, fruit nutriment rich in and biological active matter
Matter can be widely applied to many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Fructus hippophae and oil
With very high medical value, there is a cough-relieving apophlegmatic, relieving dyspepsia, promoting blood circulation to remove blood stasis effect, can treat gastric duodenal ulcer with
And indigestion etc..
Sea buckthorn fruit is full of nutrition, according to surveying and determination in its fruit containing multivitamin, fatty acid, microelement, sub- oil element,
The various amino acid of Hippophate flavone, superoxides isoreactivity substance and needed by human body.Wherein Vitamin C content is high, and every 100 grams
In fruit juice, Vitamin C content can reach 825-1100 milligrams, is 2-3 times of Kiwi berry, is known as the laudatory title of the king of vitamin C.Contain
Sugared 7.5%-10%, the 3%-5% containing acid.
Rosa roxburghii Tratt（Rosa roxbunghii）For the fruit of the perennial machaka rosa roxburghii of rosaceae also known as mountain king fruit, thorn
Certain kind of berries fruit, thatch pears etc..With clearing heat and promoting fluid, stomach strengthening and digestion promoting, relieving summer-heat and other effects.Rosa roxburghii Tratt vitamin B rich in, C, carrotene,
The ingredients such as malic acid, citric acid are usually used in deficiency of stomach-Yin, appetite stimulator, indigestion or eating accumulation, glutted full bored, diarrhea
Loose stool；Pyreticosis or hot summer weather hurt saliva, thirsty, scanty drak urine.
Mature Rosa roxburghii Tratt is fleshy hypertrophy, sweet and sour, fruit are rich in sugar, vitamin, carrotene, organic acid and more than 20 kinds of ammonia
More than base acid, 10 microelement and superoxide dismutase, especially Vitamin C content beneficial to human body are high, are to work as
It is highest in preceding fruit, it is 50 times of citrus 841.58 milligrams -3541.13 milligrams of content in every 100 grams of fresh fruits, Kiwi berry
10 times, the laudatory title with " king of vitamin C ".Fruit of Grossularia burejensis Berger has very high nutritive value and medical value, sour, puckery, flat,
Digestion-promoting spleen-invigorating restrains stopping leak, for treating having indigestion abdominal distension, dysentery, enteritis, vitamin C deficiency etc..
Wintercherry（Physali alkekengiL.）Also known as groundcherry, franchet groundcherry persistent calyx or fruit, cape gooseberry etc..Containing more in Fruit of Physalis
Kind of nutritional ingredient, wherein the content of calcium be 73.1 times of tomato, 13.8 times of carrot, ascorbic content is tomato
6.4 times, 5.4 times of carrot.With unique flavor and nutrition abundant.Fruit of Physalis aromatic flavour, delicious.Berry is rich
Containing vitamin C, beta carotene, more than 20 kinds of mineral elements and 18 kinds of amino acid, there is heat-clearing, removing toxic substances, diuresis effect is usually used in
Control heat-syndrome cough, pharyngalgia, jaundice, dysentery, in water, the treatment of furunculosis, erysipelas etc..
Blackcurrant scientific name elements of black currant（Ribes nigrum L.）Also known as currant, purple plum etc., ripening fruits are black
Small berries, it is interior to be rich in vitamin C, anthocyanidin.Blackcurrant is not only a kind of delicious flavour, fruit full of nutrition, and has
Good medicinal efficacy.Blackcurrant fruit is rich in mineral matter element, and the Zn content in mineral matter element is high, can be used as and comes for zinc
Source；Ascorbic content is high, hence it is evident that is higher than citrus, hawthorn, also contains phosphorus, magnesium, potassium, calcium, anthocyanidin, phenolic substances.Seed
Linoleic acid content is high.Ascorbic content is very rich in blackcurrant, it is often more important that is also melted into containing a large amount of antioxygen
Point, this assures ascorbic function and effect.Blackcurrant is rich in such as anthocyanidin, vitamin C, flavones, Quercetin, red bayberry
The substances such as alcohol, phenolic acid, catechin and blackcurrant polysaccharide, these are all the bioactive substances with good anti-oxidation function.
Cactus（Scientific name：Opuntia stricta(Haw.)Haw.var.dillenii(Ker-Gawl.) Benson）,
It is a kind of plant of Opuntia.Alias celestial being palm, kwan-yin palm etc..Cactus is the meat shrub that grows thickly, the wide ovum of top branch
Shape, obovate elliptic or subcircular；Flower spoke shape, holder obovate.It is used as medicine with complete stool.The four seasons can adopt.
Cactus is cool in nature, sour, nontoxic.Have the promoting flow of qi and blood circulation, heat-clearing toxin-expelling functions.Mainly have clearing heat and detoxicating, eliminating stasis to subdue swelling,
The functions such as the promoting flow of qi and blood circulation, benefiting stomach and stopping pain.Cactus contains amino acid and various trace elements in needed by human 8, and embraces green
The preciousness ingredient such as lotus, Kok Beattie cents, cholla stem, starches through cactus crude drug and analyzes, contain pectin, polysaccharide and glue exudate.From herb point
From extracting to obtain freidelin ketone（friedelin）, -3 α -ol of freidelin（friedelan-3-α-ol）, taraxerone
（taraxerone）And taraxerol（taraxerol）.
Eggshell, quantity is biggish in China's poultry farming chicken, duck, goose and quail etc., using egg, duck's egg,
As in food processing process, the useless eggshell enormous amount generated is provided according to existing disclosed report for goose egg and quail egg
Material display, the eggshell total amount that a medium-sized city is monthly discarded reach 60-100 tons, and account for egg size by Eggshell weight 11% calculates,
The eggshell that about 1,500,000 tons of the daily output in China, not only pollutes environment, but also waste of resource in this way.Discarded eggshell is integrated
It utilizes, resource utilization can not only be improved and avoids environmental pollution, and be greatly improved the economic benefit and social benefit of enterprise.
Wherein egg shell is in China's yield maximum, and egg shell accounts for the 10-12% in entire egg, by under film, shell on shell
Film and eggshell three parts composition, eggshell is a kind of calcareous hard shell, is located on shell between film and shell lower film.In the change of egg shell
In studying point, minerals account for 95%, and protein 3.3%, moisture 1%, fat 0.03%, remaining is carbohydrate and other have
Machine substance（It certainly, does not include a large amount of remaining egg white in eggshell in the above ingredient）.Eggshell mineral ingredient is mainly
Calcium carbonate accounts for 94% or so, remaining is magnesium carbonate and calcium phosphate, and calcium, phosphorus content account for 34.9% and of eggshell dry matter respectively
0.2%.In eggshell, seven kinds of vitamins and various trace elements and some important enzymes, peptide matters can be measured, he
Although content seldom, be all the substance needed in human body or animal body.
In the prior art, it is replenished the calcium with egg-shell meal, has the product of the grinds after sterilizing by eggshell in people's product, also have
The application of organic calcium is prepared into using primary or secondary clacining method.It is at present most widely in drug, food and nutriment
In preparation, micron order, submicron order and nano level superfine powder are mostly used, is added to various food, nutrition kind, has reached very
Good effect.Application of the eggshell in China's animal-breeding is taken mostly by eggshell cleaning, drying, and milling is added into feed
The method of feeding, since grinding particle size is larger, being absorbed and utilized for animal is poor, can't reach good application effect.
The present invention is first to be ground into coarse powder by after eggshell cleaning, high temperature drying, fermented using saccharomycete, then again
Carbonyl ammonia reaction treatment is carried out, then dried, crush and be prepared into biological calcium product, the program not only improves what eggshell utilized
Nutrition, while the main composition-calcium carbonate in eggshell being made to obtain biochemical reaction, the biological calcium carbonate for being more conducive to absorb is formed,
The reaction of carbonyl ammonia makes its protein component be further converted to amino acid again, makes the protein, amino acid converting for small peptide of macromolecular
Or the small-molecule substances such as small peptide, the beneficial effect for reaching collection eutrophy and being absorbed and utilized.
The beneficial effects of the invention are as follows：
（1）Product of the present invention is single type bacillus laterosporus feed addictive, can be had to producer to product more clear
Demand selection, meanwhile, also can preferably reach the application purpose of quantization as needed.Product can highlight bacillus laterosporus
Viable bacteria content.Survival rate of the thallus in closed composite packaging is significantly improved, keep sample sight at room temperature through batches of products
It examines 12 months, bacillus laterosporus average viability is 95.7%, and reference substance（The filler of reference substance is wheat bran）In side
Spore bacillus survival rate is 87%；
（2）Pass through sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder and side spore bud
Spore bacillus mixes, and due to the stickum in the colloidal substance that these dry powder formulations are rich in, especially cactus, can preferably increase
Add the adhesive effect between additive and feed, and improves the utilization rate of additive；
（3）By sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder high-content amino acid, vitamin, mineral element, more
Sugar.The nutritional ingredients such as the vitamin C of organic acid, especially high-content and various bioactivators, to raising animal immune energy
Power is simultaneously obviously improved meat, Egg Quality, the acidic materials in especially above-mentioned dry powder, utilization, promotion animal to contained microorganism
Digestion all has significant effect；
（4）Due to using Freeze Drying Technique means, enable sea-buckthorn obtained, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder
Its active constituent is maximumlly remained, has distinctive sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the smell of cactus application and taste
Taste, free from extraneous odour add the preference of animal.
（5）Using egg-shell meal first through saccharomycetes to make fermentation, carbonyl ammonia is then used（Maillard reaction）Reaction is prepared into rich in life
The fermentation egg-shell meal of object calcium not only makes the calcium carbonate in eggshell obtain the BIOLOGICAL CALCIUM that bioconversion obtains being conducive to animal absorption,
And further converted to a large amount of protein, amino acid, carbohydrate is formed after saccharomycetes to make fermentation, make amino
Acid, small peptide or small peptide small-molecule substance get a promotion, and more conducively animal is absorbed and utilized, and pass through what conversion carbohydrate was formed
Inverted sugar and fragrance matter further improve the flavor and taste of product, increase animal to the preference of product.
（6）To the preparation of sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder in the present invention, do not remove the peel, deseeding step
Suddenly, it is therefore an objective to by being crushed after freeze-drying, its skin and seed is made to wear into fine powder, retain anthocyanidin in its skin and seed,
Oils, organic acid, bud fragrance substance are retained, and the nutritive value of its freeze-dried powder is promoted.
（7）The present invention is lyophilized using sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus
Powder, matrix of the fermentation egg-shell meal as additive contain constants such as a large amount of calcium, phosphorus, potassium, sodium, magnesium, zinc and micro in matrix
Element, vitamin C still rich in, anthocyanidin, beta carotene, malic acid, citric acid, protein, amino acid, nucleic acid, Portugal
Grape sugar, lipoidis, carbohydrate, B family vitamin such as B1、B2、B6、B12And the physiological activity such as folic acid, niacin, pantothenic acid, inositol
Substance, not only improves the nutritive value of bacillus laterosporus additive, while promoting the metabolism of animal, improves the anti-of body
Sick ability promotes growth of animal, finally improves the economic benefit of its aquaculture.
（8）Product of the present invention uses interior outer bag composite packaging, and interior outer bag interlayer is filled with silica-gel desiccant, and product does not return
Tide, no agglomeration, product is loose, good fluidity, can preferably keep exterior quality and inherent quality.
Operation to not having its technical process stated in detail in the present invention is in the common sense of those skilled in the art
Operation.With regard to the purchase and storage of heretofore described sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant fresh fruit and fresh cactus, the present invention
The construction of people's unit has 20,000 tons of freezer facility, has enough guarantees to storage fresh fruit material.
Specific embodiment
Embodiment 1
The preparation of sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder：
Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the cactus for taking fresh mature respectively remove carpopodium, the impurity such as rotten fruit, with containing heavy
40 DEG C of aqueous solutions of 0.1% citric acid are measured, 15min is rinsed under constant stirring and pulls out, be put into pure water and rinse 3 times, pull out
It is drained；It will be moved into refrigerator after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively, be cooled to -45 DEG C rapidly
Below；Respectively by the sea-buckthorn freezed, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus move into lyophilization machine in, sublimation temperature control-
22--32 DEG C, vacuum degree 120-150Pa makes dry materials to moisture < 6%（w）, the time about needs 18 hours, is put into after taking-up
In freezing crusher, control temperature of charge is no more than 0 DEG C, after being crushed to 100 mesh of fineness >, respectively obtains sea-buckthorn freeze-dried powder, thorn
Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are fitted into polybag and seal, and are stored in 0 DEG C or less
It is spare in freezer.
Embodiment 2
Using the culture medium accordingly optimized, candida utili, torrid zone vacation are individually cultivated using Liquid Culture Zymolysis Equipment
Silk yeast, brewer's yeast, brewer's yeast and fodder yeast, are then separated the thallus in fermentation liquid with supercentrifuge,
It is with vacuum freeze drying mode that bacterium mud is dry, respectively obtain candida utili dry powder that viable bacteria content is 200 hundred million/grams,
Candida tropicalis dry powder that viable bacteria content is 200 hundred million/grams, the brewer's yeast dry powder that viable bacteria content is 200 hundred million/grams, work
The feed yeast powder that the brewer's yeast dry powder and viable bacteria content that bacterial content is 200 hundred million/grams are 200 hundred million/grams.
Embodiment 3
The preparation of fermentation egg-shell meal：
One or more of mixing eggshells of egg shell or Ovum Anas domestica putamina, goose egg-shell, quail eggshell are cleaned up, moisture is dried, sets
Enter in baking oven, under the conditions of 240 DEG C after heated-air drying 1.5 hours, taking-up is let cool, and is ground into 45 mesh powder；It is public to weigh egg-shell meal 80
Jin, suitable pure water, which is added, makes into material moisture 50%（W）, add the feed yeast powder 20 that embodiment 2 obtains
Kilogram, rear sabot is mixed well, fermenting cellar is placed in, is fermented 60-72 hours under the conditions of temperature is 31-33 DEG C, to fermentation ends
Afterwards, fermentation materials are adjusted to temperature is 82 DEG C -92 DEG C, under the conditions of temperature is 80-90%, carries out carbonyl ammonia and reacts 22-28 days, so
Material is dried to moisture content < 5% under the conditions of being not more than 100 DEG C using electric baking case afterwards（W）, utilize omnipotent crushing
Machine is crushed, and crosses 100 sieves to get fermentation egg-shell meal.
With the preparation method of the fermentation egg-shell meal of embodiment 3, it is dry that candida utili obtained in embodiment 2 can be respectively adopted
Powder, candida tropicalis dry powder, brewer's yeast dry powder or brewer's yeast dry powder carry out preparing fermented yeast egg-shell meal, other techniques
Condition is implemented all in accordance with eggshell powder preparation technique parameter of fermenting in the claim of this application 1-4.
Embodiment 4
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 10000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5g, Rosa roxburghii freeze-dried powder
50g, wintercherry freeze-dried powder 3g, blackcurrant freeze-dried powder 3g, cactus freeze-dried powder 150g, remaining is fermentation egg-shell meal；Specific production side
Method is：Weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being respectively
It slaps freeze-dried powder to mix, then bacillus laterosporus dry powder uniformly be admixed, then add surplus and ferment as made from embodiment 3 eggshell
After powder is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag,
Add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 5
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 30000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 45g, Rosa roxburghii Tratt freeze-drying
Powder 18g, wintercherry freeze-dried powder 5g, blackcurrant freeze-dried powder 5g, cactus freeze-dried powder 50g, remaining is fermentation egg-shell meal；Specific production side
Method is the same as embodiment 4.
Embodiment 6
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 50000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 30g, Rosa roxburghii Tratt freeze-drying
Powder 25g, wintercherry freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 85g, remaining is fermentation egg-shell meal；Specific production
Method is the same as embodiment 4.
Embodiment 7
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 80000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii Tratt freeze-drying
Powder 25g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 100g, remaining is fermentation egg-shell meal；Specific system
Make method with embodiment 4.
Embodiment 8
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 85000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii Tratt freeze-drying
Powder 25g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 120g, remaining is fermentation egg-shell meal；Specific system
Make method with embodiment 4.
Embodiment 9
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 90000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 35g, Rosa roxburghii Tratt freeze-drying
Powder 35g, wintercherry freeze-dried powder 20g, blackcurrant freeze-dried powder 20g, cactus freeze-dried powder 115g, remaining is fermentation egg-shell meal；Specific system
Make method with embodiment 4.
Embodiment 10
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 100000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii Tratt freeze-drying
Powder 30g, wintercherry freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 150g, remaining is fermentation egg-shell meal；Specific system
Make method with embodiment 4.
Embodiment 11
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 110000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 45g, Rosa roxburghii Tratt freeze-drying
Powder 35g, wintercherry freeze-dried powder 25g, blackcurrant freeze-dried powder 25g, cactus freeze-dried powder 135g, remaining is fermentation egg-shell meal；Specific system
Make method with embodiment 4.
Embodiment 12
A kind of bacillus laterosporus feed addictive is by bacillus laterosporus dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：The bacillus laterosporus 120000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii Tratt freeze-drying
Powder 50g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 50g, remaining is fermentation egg-shell meal；Specific production
Method is the same as embodiment 4.
Claims (10)
1. a kind of bacillus laterosporus feed addictive, it is characterized in that being frozen first by bacillus laterosporus, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt
Dry powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus
Uniformly, composite packaging is made：Bacillus laterosporus 10000 hundred million -120000 hundred million, sea-buckthorn in every 1000g feed addictive
Freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-
150g, remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being
People slaps freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively
The palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；It is described above
Rinsing condition be to be rinsed 15min, and be stirred continuously, pulled out and be put into pure water with the citric acid of 40 DEG C of water addition weight 0.1%
Middle rinsing 3 times；Above-mentioned freezing be will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively -45 DEG C with
Under；The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, vacuum degree 120-150Pa, it is dry to moisture less than 6%/
Until w；The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours, then crushes in advance
At 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed
It is even, it ferments 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, it is 82- that fermentation materials, which are adjusted to temperature,
92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case be not more than
It is dried to moisture content < 5%/w under the conditions of 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
2. a kind of bacillus laterosporus feed addictive, it is characterized in that being frozen first by bacillus laterosporus, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt
Dry powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus
Uniformly, composite packaging is made：Bacillus laterosporus 30000 hundred million -120000 hundred million, sea-buckthorn in every 1000g feed addictive
Freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 8-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder
70-150g, remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder,
Cactus freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively
People's palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Above-mentioned institute
The rinsing condition stated is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pull out be put into it is pure
It is rinsed 3 times in water；Above-mentioned freezing is will to be cooled to rapidly -45 DEG C after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively
Below；The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dried to moisture and is less than
Until 6%/w；The fermentation egg-shell meal, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, then
It is ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the yeast of egg-shell meal weight 20%
Bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials, which are adjusted to temperature, is
82-92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case not
It is dried to moisture content < 5%/w under the conditions of greater than 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
3. a kind of bacillus laterosporus feed addictive, it is characterized in that being frozen first by bacillus laterosporus, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt
Dry powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus
Uniformly, composite packaging is made：Bacillus laterosporus 50000 hundred million -120000 hundred million, sea-buckthorn in every 1000g feed addictive
Freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 12-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder
85-150g, remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder,
Cactus freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively
People's palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Above-mentioned institute
The rinsing condition stated is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pull out be put into it is pure
It is rinsed 3 times in water；Above-mentioned freezing is will to be cooled to rapidly -45 DEG C after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively
Below；The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dried to moisture and is less than
Until 6%/w；The fermentation egg-shell meal, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, then
It is ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the yeast of egg-shell meal weight 20%
Bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials, which are adjusted to temperature, is
82-92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case not
It is dried to moisture content < 5%/w under the conditions of greater than 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
4. a kind of bacillus laterosporus feed addictive, it is characterized in that being frozen first by bacillus laterosporus, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt
Dry powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus
Uniformly, composite packaging is made：Bacillus laterosporus 80000 hundred million -120000 hundred million, sea-buckthorn in every 1000g feed addictive
Freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-drying
Powder 100-150g, remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-drying
Powder, cactus freeze-dried powder, are made by following methods, technical process be take respectively fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant,
Cactus removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；It is above-mentioned
The rinsing condition is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pull out be put into it is pure
It is rinsed 3 times in water purification；Above-mentioned freezing is will to be cooled to rapidly -45 after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively
DEG C or less；The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dried to moisture and is less than
Until 6%/w；The fermentation egg-shell meal, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, then
It is ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the yeast of egg-shell meal weight 20%
Bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials, which are adjusted to temperature, is
82-92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case not
It is dried to moisture content < 5%/w under the conditions of greater than 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
5. a kind of bacillus laterosporus feed addictive as described in claim 1-4, which is characterized in that the side spore gemma bar
Bacterium is no less than 200 hundred million thalli dry powder products for every gram containing viable bacteria concentration.
6. a kind of bacillus laterosporus feed addictive as described in claim 1-4, which is characterized in that the low-temperature grinding is
Temperature of charge is in 0 DEG C or less and is crushed.
7. a kind of bacillus laterosporus feed addictive as described in claim 1-4, which is characterized in that the composite packaging is adopted
With interior outer bag composite packaging, inner bag uses polypropylene plastics pocket, and outer packing uses aluminium foil bag, it is dry that silica gel is filled between interior outer bag
Drying prescription pouch.
8. a kind of bacillus laterosporus feed addictive as described in claim 1-4, which is characterized in that the eggshell is egg
Any one or a few mixing eggshell in shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell.
9. a kind of bacillus laterosporus feed addictive as described in claim 1-4, which is characterized in that described is dry using heat
Air-dry dry or far-infrared ray drying；The drying condition is 180 DEG C -300 DEG C.
10. a kind of bacillus laterosporus feed addictive as described in claim 1-4, which is characterized in that the saccharomycete is
Any one in candida utili, candida tropicalis, brewer's yeast, brewer's yeast and fodder yeast.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810511905.3A CN108850473A (en) | 2018-05-25 | 2018-05-25 | A kind of bacillus laterosporus feed addictive |
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| CN201810511905.3A CN108850473A (en) | 2018-05-25 | 2018-05-25 | A kind of bacillus laterosporus feed addictive |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158653A (en) * | 2021-06-24 | 2022-03-11 | 山东绿都安特动物药业有限公司 | Additive premix feed for improving non-specific immune function of weaned piglets |
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2018
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158653A (en) * | 2021-06-24 | 2022-03-11 | 山东绿都安特动物药业有限公司 | Additive premix feed for improving non-specific immune function of weaned piglets |
| CN114158653B (en) * | 2021-06-24 | 2023-10-24 | 山东绿都安特动物药业有限公司 | Additive premix feed for improving nonspecific immunity of weaned pigs |
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