CN108850480A - A kind of enterococcus faecium feed addictive - Google Patents

Abstract

A kind of enterococcus faecium feed addictive of the present invention, it is characterized in that being uniformly mixed by enterococcus faecium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, the fermentation egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made：Enterococcus faecium 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is fermentation egg-shell meal.The present invention is single type enterococcus faecium feed addictive, and clear containing viable count, has specific application and Quantitatively Selecting conducive to user.The present invention is using sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder and fermentation egg-shell meal as the matrix of additive, not only improve the nutritive value of enterococcus faecium additive, promote the metabolism of animal simultaneously, improve the disease resistance of body, promote growth of animal, finally improves the economic benefit of its aquaculture.

Description

A kind of enterococcus faecium feed addictive

Technical field

The invention belongs to feed additive fields, are related to a kind of for cultivated animals probiotic feed additive.Specifically It is related to a kind of enterococcus faecium feed addictive.

Background technique

Enterococcus faecium is living microorganism, can be acted on by the competitive exclusion of the alimentary canal biology of animal, inhibit to have The growth of evil microbial bacteria forms dominant microflora or prevents disease by enhancing non-specific immune function, to promote dynamic Object grows and improves food conversion ratio.

Enterococcus faecium（Enterococcus Faecium）It is that Minister Agriculture of China announces No. 2045 announcement《Feed addition Agent catalogue（2013）》It is allowed for the microorganism of cultivated animals, with regard to the dosage form that current enterococcus faecium is used as additive, there is water Agent and pulvis two major classes, aqueous solution dosage form is because the nutritional substance in thallus aqua easy to use continues to be metabolized, growth fermentation, Therefore, the thallus of this dosage form plays pendulum, and storage life is shorter.Powder formulation is then that the thallus lived is utilized Low Temperature Thermal Thallus is dehydrated at a slow speed by the drying means such as wind, freezing, and thallus is made to be in stable drying dormant state, and recycling can be with Feeding substance is uniformly mixed as filler and relatively stable powder-type biological feed additive is made.But just production at present Enterprise in view of raw material be easy to get and processing cost, often select rice bran, wheat bran, corn flour, maize cob meal, peanut shell powder, shellfish One or more of shell powder, vermiculite power, mountain flour, calcium carbonate, medical stone, zeolite powder mixture, some also use straw Powder or grass meal are as filler material.The considerations of these are in line with production cost then has ignored the stability and feed addition of microbial bacteria The trophism of agent is worth.

In order in use, have certain adhesion, microbial bacteria feed addictive be made to exist in microbial bacteria additive It admixes when being mixed in animal feed, can be adsorbed on animal feed, convenient for the uniformity in animal feeding and be fed into bacterium amount Maximization, some products take pains a certain amount of binder of addition.

In order to make to raise the relative constant of animal stomach inner pH value, acid is continued up to after so that the foodstuff being fed into is entered small intestine The effect of change, in the product often simultaneously using acidulants such as citric acid, lactic acid, hydrochloric acid, fumaric acid, but these acidulants are not only straight It connects and locally causes acidity too strong microbial bacteria contained by additive, influence the survival of thallus, meanwhile, these acidifying substances are to cultivation Animal also without the nutritive value directly utilized, meanwhile, there is very big irritation to the gastrointestinal tract of the cubs of livestock and poultry, chick, Compatibility is using not ideal enough.

Above-mentioned deficiency can be overcome by developing one kind, can either keep stability, the trophism of institute's mycetome, be had again to thallus There is adhesion, while having again to the filler used and providing the additive for microbe feedstuff of nutritive value to cultivated animals, is Promote the basis of feed additive industry long-term stability development.

Summary of the invention

The purpose of the present invention is to provide a kind of new single type enterococcus faecium feed addictive, which adds Adding agent not only, there is thallus to stablize, and the thallus time-to-live is long, and used auxiliary agents have certain adhesiveness and suction Attached property can make thallus preferably be bonding on one with auxiliary agents, meanwhile, complementary additive has certain acidity, right After animal feeding, nutriment abundant can not only be provided, while to be able to maintain stomach inner pH value relative constant for its acidic materials, enters The effect of acidification is continued up to after small intestine.The purpose is to provide the enterococcus faecium feed addition of a kind of more stability and trophism Agent.

A kind of enterococcus faecium feed addictive of the present invention, it is characterized in that by enterococcus faecium and a certain amount of seabuckthorn fruit powder, Rosa roxburghii Tratt Powder, wintercherry powder, blackcurrant powder, cactus powder and fermentation egg-shell meal uniformly mix, and obtain a kind of new enterococcus faecium feed addition The thallus of agent, this additive is stablized, and the thallus time-to-live is long, and seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant powder sugar content Height, especially cactus powder all have preferable Adhesion property, and fermentation egg-shell meal also has preferable adsorptivity.Wherein sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant are the ascorbic fruits of high-content, and acidity is extremely strong, not only provide dimension abundant to cultivated animals The nutriments such as element are given birth to, the acidic materials after feeding play the effect for promoting digestion well, to highlight the present invention Purpose.

In order to realize the present invention, the following technical solutions are specifically adopted：

A kind of enterococcus faecium feed addictive, feature it be first by enterococcus faecium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple Packaging is closed to be made：Enterococcus faecium 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is Ferment egg-shell meal.

Enterococcus faecium described above is enterococcus faecium dry powder product.

The enterococcus faecium dry powder product can be used and commercially available be used as every gram of feed addictive containing viable bacteria concentration The weight proportion of no less than 250 hundred million preparations, the enterococcus faecium dry powder product and the filler material in formula is done by thallus The thallus content of powder and final products determines.

The enterococcus faecium dry powder product also can use the culture medium that existing method selection accordingly optimizes and pass through liquid Then Zymolysis Equipment culture is separated the thallus in fermentation liquid with high speed centrifugation mode, be placed in dry indoor cold wind or Bacterium mud drying is made, every gram of viable bacteria concentration no less than 250 hundred million thalli dry powders of acquisition with vacuum freeze drying mode Product.

Sea-buckthorn freeze-dried powder described above, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively, The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing condition described above is With the citric acid of 40 DEG C of water addition weight 0.1%, 15min is rinsed, and be stirred continuously, pulls out to be put into pure water and rinse 3 times；On Stating freezing is that will be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；Above-mentioned distillation Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；It is above-mentioned Low-temperature grinding is the crushing that temperature of charge is in 0 DEG C or less progress, 100 mesh of grinding particle size >.

Composite packaging described above uses interior outer bag composite packaging, and inner bag uses polypropylene plastics pocket, and outer packing uses aluminium Foil bag is filled with silica-gel desiccant pouch between interior outer bag.

Fermentation egg-shell meal described above, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, so After be ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the ferment of egg-shell meal weight 20% Female bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials is adjusted to temperature Carbonyl ammonia is carried out under the conditions of being 80-90% for 82-92 DEG C, humidity（Also referred to as Maillard reaction）Reaction 22-28 days, then, by material Moisture content < 5%/w is dried under the conditions of being not more than 100 DEG C using electric baking case, is crushed, is sieved with 100 mesh sieve to obtain the final product.

Drying described above uses heated-air drying or far-infrared ray drying；Dry condition is 180 DEG C -300 DEG C, drying Purpose kill the harmful substances such as microorganism and helminth first is that reaching, second is that the crisp matterization of eggshell can be made to be conducive to crush, and make powder Egg-shell meal after broken is loose conducive to fermentation.

The carbonyl ammonia reaction, i.e. Maillard reaction, also known as " non enzymatic browning reacton ", are carbonyls（Reduced sugar Class）And amino-compound（Amino acid and protein）Between reaction.

The above-mentioned purpose that eggshell coarse powder is used to saccharomycetes to make fermentation, first is that can effectively make eggshell sufficiently soft using fermentation process Change, the egg-shell meal after making fermentation is loose, is easy to mix with unclassified stores；Second is that can effectively be made in eggshell using saccharomycetes to make fermentation The ingredients such as calcium obtain effective bioconversion, form BIOLOGICAL CALCIUM, conducive to being absorbed and utilized for animal, saccharomycetes to make fermentation can make fermentation material Material forms the mass propagation of more protein, especially saccharomycete, and it is peculiar to increase yeast institute for the aggregation for the ingredient that can have additional nutrients The multiple nutritional components such as glucose, protein, nucleic acid, vitamin, carbohydrate, lipid-like substances, especially B race dimension life Element such as B₁、B₂、B₆、B₁₂And the physiological activators such as folic acid, niacin, pantothenic acid, inositol get a promotion, these beneficial nutrients Matter ingredient undoubtedly plays the nutritional ingredient for improving product of the present invention, improves its nutritive validity.

The further carbonyl ammonia of egg-shell meal is reacted in the present invention, first is that promoting the maturing process of eggshell, is further made wherein Calcareous ingredient obtain sufficient bioconversion, biological calcium component is formed, second is that obtaining the protein component in material sufficiently Bioconversion, amino acid is degraded to, third is that a large amount of aminoacid ingredients to be formed is made to be converted into the small molecules object such as small peptide and small peptide Matter, conducive to digesting and absorbing after animal feeding, the reaction of carbonyl ammonia not only promotes the trophic function of product, after the reaction of carbonyl ammonia Material can further increase the application that gas porosity is conducive to mix feed, while carbohydrate being made to be converted into reduced sugar, produce simultaneously Raw fragrance matter abundant, not only improves the anti-immunity ability of product, further improves the flavor of product, increase being fond of for animal Property.

Eggshell described above is the mixed of any one or a few in egg shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell Close eggshell.

Saccharomycete described above（saccharomyces）For candida utili（Candida utilis）, the torrid zone it is false Silk yeast（Candida tropicalis）, brewer's yeast（Saccharomyces cerevisiae）, brewer's yeast （Saccharomyces cerevisiae）And fodder yeast（Candida Tropicali）In any one.

Above-mentioned saccharomycete can choose using all viable bacterias as food additives or feed addictive commercially available Concentration is no less than 200 every gram hundred million thalli dry powder products.

Existing method preparation can also be used in above-mentioned saccharomycete, such as can accordingly be optimized according to the selection of the property of strain Then thallus in fermentation liquid is separated with high speed centrifugation mode, is set by the culture of Liquid Culture Zymolysis Equipment by culture medium Bacterium mud drying is made in the indoor cold air drying of drying or with freeze-drying mode, obtaining viable bacteria concentration respectively is every gram 200 hundred million thalli dry powder products.

A kind of prioritization scheme of enterococcus faecium feed addictive of the present invention is, first by enterococcus faecium, sea-buckthorn freeze-dried powder, thorn Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal of surplus It is uniformly mixed, composite packaging is made：Enterococcus faecium 50000 hundred million -150000 hundred million, sea-buckthorn in every 1000g feed addictive Freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 10-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70-150g, remaining is fermentation egg-shell meal.

The further prioritization scheme of the present invention is a kind of enterococcus faecium feed addictive, it is characterized in that first by dung intestines ball Bacterium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add The fermentation egg-shell meal of surplus is uniformly mixed, and composite packaging is made：The enterococcus faecium 70000 hundred million-in every 1000g feed addictive 150000 hundred million, sea-buckthorn freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8- 30g, cactus freeze-dried powder 85-150g, remaining is fermentation egg-shell meal.

Further optimization plan of the present invention is a kind of enterococcus faecium feed addictive, it is characterized in that first by dung intestines Coccus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add The fermentation egg-shell meal of surplus is added to be uniformly mixed, composite packaging is made：The enterococcus faecium 90000 in every 1000g feed addictive Hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 20-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 100-150g, remaining is fermentation egg-shell meal.

Sea-buckthorn（Hippophae rhamnoides Linn.）, fruit nutriment rich in and biological active matter Matter can be widely applied to many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Fructus hippophae and oil With very high medical value, there is a cough-relieving apophlegmatic, relieving dyspepsia, promoting blood circulation to remove blood stasis effect, can treat gastric duodenal ulcer with And indigestion etc..

Sea buckthorn fruit is full of nutrition, according to surveying and determination in its fruit containing multivitamin, fatty acid, microelement, sub- oil element, The various amino acid of Hippophate flavone, superoxides isoreactivity substance and needed by human body.Wherein Vitamin C content is high, and every 100 grams In fruit juice, Vitamin C content can reach 825-1100 milligrams, is 2-3 times of Kiwi berry, is known as the laudatory title of the king of vitamin C.Contain Sugared 7.5%-10%, the 3%-5% containing acid.

Rosa roxburghii Tratt（Rosa roxbunghii）For the fruit of the perennial machaka rosa roxburghii of rosaceae also known as mountain king fruit, thorn Certain kind of berries fruit, thatch pears etc..With clearing heat and promoting fluid, stomach strengthening and digestion promoting, relieving summer-heat and other effects.Rosa roxburghii Tratt vitamin B rich in, C, carrotene, The ingredients such as malic acid, citric acid are usually used in deficiency of stomach-Yin, appetite stimulator, indigestion or eating accumulation, glutted full bored, diarrhea Loose stool；Pyreticosis or hot summer weather hurt saliva, thirsty, scanty drak urine.

Mature Rosa roxburghii Tratt is fleshy hypertrophy, sweet and sour, fruit are rich in sugar, vitamin, carrotene, organic acid and more than 20 kinds of ammonia More than base acid, 10 microelement and superoxide dismutase, especially Vitamin C content beneficial to human body are high, are to work as It is highest in preceding fruit, it is 50 times of citrus 841.58 milligrams -3541.13 milligrams of content in every 100 grams of fresh fruits, Kiwi berry 10 times, the laudatory title with " king of vitamin C ".Fruit of Grossularia burejensis Berger has very high nutritive value and medical value, sour, puckery, flat, Digestion-promoting spleen-invigorating restrains stopping leak, for treating having indigestion abdominal distension, dysentery, enteritis, vitamin C deficiency etc..

Wintercherry（Physali alkekengiL.）Also known as groundcherry, franchet groundcherry persistent calyx or fruit, cape gooseberry etc..Containing more in Fruit of Physalis Kind of nutritional ingredient, wherein the content of calcium be 73.1 times of tomato, 13.8 times of carrot, ascorbic content is tomato 6.4 times, 5.4 times of carrot.With unique flavor and nutrition abundant.Fruit of Physalis aromatic flavour, delicious.Berry is rich Containing vitamin C, beta carotene, more than 20 kinds of mineral elements and 18 kinds of amino acid, there is heat-clearing, removing toxic substances, diuresis effect is usually used in Control heat-syndrome cough, pharyngalgia, jaundice, dysentery, in water, the treatment of furunculosis, erysipelas etc..

Blackcurrant scientific name elements of black currant（Ribes nigrum L.）Also known as currant, purple plum etc., ripening fruits are black Small berries, it is interior to be rich in vitamin C, anthocyanidin.Blackcurrant is not only a kind of delicious flavour, fruit full of nutrition, and has Good medicinal efficacy.Blackcurrant fruit is rich in mineral matter element, and the Zn content in mineral matter element is high, can be used as and comes for zinc Source；Ascorbic content is high, hence it is evident that is higher than citrus, hawthorn, also contains phosphorus, magnesium, potassium, calcium, anthocyanidin, phenolic substances.Seed Linoleic acid content is high.Ascorbic content is very rich in blackcurrant, it is often more important that is also melted into containing a large amount of antioxygen Point, this assures ascorbic function and effect.Blackcurrant is rich in such as anthocyanidin, vitamin C, flavones, Quercetin, red bayberry The substances such as alcohol, phenolic acid, catechin and blackcurrant polysaccharide, these are all the bioactive substances with good anti-oxidation function.

Cactus（Scientific name：Opuntia stricta(Haw.)Haw.var.dillenii(Ker-Gawl.) Benson）, It is a kind of plant of Opuntia.Alias celestial being palm, kwan-yin palm etc..Cactus is the meat shrub that grows thickly, the wide ovum of top branch Shape, obovate elliptic or subcircular；Flower spoke shape, holder obovate.It is used as medicine with complete stool.The four seasons can adopt.

Cactus is cool in nature, sour, nontoxic.Have the promoting flow of qi and blood circulation, heat-clearing toxin-expelling functions.Mainly have clearing heat and detoxicating, eliminating stasis to subdue swelling, The functions such as the promoting flow of qi and blood circulation, benefiting stomach and stopping pain.Cactus contains amino acid and various trace elements in needed by human 8, and embraces green The preciousness ingredient such as lotus, Kok Beattie cents, cholla stem, starches through cactus crude drug and analyzes, contain pectin, polysaccharide and glue exudate.From herb point From extracting to obtain freidelin ketone（friedelin）, -3 α -ol of freidelin（friedelan-3-α-ol）, taraxerone （taraxerone）And taraxerol（taraxerol）.

Eggshell, quantity is biggish in China's poultry farming chicken, duck, goose and quail etc., using egg, duck's egg, As in food processing process, the useless eggshell enormous amount generated is provided according to existing disclosed report for goose egg and quail egg Material display, the eggshell total amount that a medium-sized city is monthly discarded reach 60-100 tons, and account for egg size by Eggshell weight 11% calculates, The eggshell that about 1,500,000 tons of the daily output in China, not only pollutes environment, but also waste of resource in this way.Discarded eggshell is integrated It utilizes, resource utilization can not only be improved and avoids environmental pollution, and be greatly improved the economic benefit and social benefit of enterprise.

Wherein egg shell is in China's yield maximum, and egg shell accounts for the 10-12% in entire egg, by under film, shell on shell Film and eggshell three parts composition, eggshell is a kind of calcareous hard shell, is located on shell between film and shell lower film.In the change of egg shell In studying point, minerals account for 95%, and protein 3.3%, moisture 1%, fat 0.03%, remaining is carbohydrate and other have Machine substance（It certainly, does not include a large amount of remaining egg white in eggshell in the above ingredient）.Eggshell mineral ingredient is mainly Calcium carbonate accounts for 94% or so, remaining is magnesium carbonate and calcium phosphate, and calcium, phosphorus content account for 34.9% and of eggshell dry matter respectively 0.2%.In eggshell, seven kinds of vitamins and various trace elements and some important enzymes, peptide matters can be measured, he Although content seldom, be all the substance needed in human body or animal body.

In the prior art, it is replenished the calcium with egg-shell meal, has the product of the grinds after sterilizing by eggshell in people's product, also have The application of organic calcium is prepared into using primary or secondary clacining method.It is at present most widely in drug, food and nutriment In preparation, micron order, submicron order and nano level superfine powder are mostly used, is added to various food, nutrition kind, has reached very Good effect.Application of the eggshell in China's animal-breeding is taken mostly by eggshell cleaning, drying, and milling is added into feed The method of feeding, since grinding particle size is larger, being absorbed and utilized for animal is poor, can't reach good application effect.

The present invention is first to be ground into coarse powder by after eggshell cleaning, high temperature drying, fermented using saccharomycete, then again Carbonyl ammonia reaction treatment is carried out, then dried, crush and be prepared into biological calcium product, the program not only improves what eggshell utilized Nutrition, while the main composition-calcium carbonate in eggshell being made to obtain biochemical reaction, the biological calcium carbonate for being more conducive to absorb is formed, The reaction of carbonyl ammonia makes its protein component be further converted to amino acid again, makes the protein, amino acid converting for small peptide of macromolecular Or the small-molecule substances such as small peptide, the beneficial effect for reaching collection eutrophy and being absorbed and utilized.

The beneficial effects of the invention are as follows：

（1）Product of the present invention can highlight the viable bacteria content of enterococcus faecium, significantly improve thallus depositing in closed composite packaging Motility rate, through batches of products reserved sample observing 12 months at room temperature, enterococcus faecium average viability is 93.7%, and reference substance （The filler of reference substance is wheat bran）In enterococcus faecium survival rate be 78.2%；Product of the present invention is the feeding of single type enterococcus faecium Feed additives can provide the selection of the specific aim and quantization with application to producer, be conducive to targeted to livestock and poultry It is convenient to use.

（2）Pass through sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder and dung Enterococcus mixes, and due to the stickum in the colloidal substance that these dry powder formulations are rich in, especially cactus, can preferably increase Add the adhesive effect between additive and feed, and improves the utilization rate of additive；

（3）By sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder high-content amino acid, vitamin, mineral element, more Sugar.The nutritional ingredients such as the vitamin C of organic acid, especially high-content and various bioactivators, to raising animal immune energy Power is simultaneously obviously improved meat, Egg Quality, the acidic materials in especially above-mentioned dry powder, utilization, promotion animal to contained microorganism Digestion all has significant effect；

（4）Due to using Freeze Drying Technique means, enable sea-buckthorn obtained, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder Its active constituent is maximumlly remained, has distinctive sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the smell of cactus application and taste Taste, free from extraneous odour add the preference of animal.

（5）Using egg-shell meal first through saccharomycetes to make fermentation, carbonyl ammonia is then used（Maillard reaction）Reaction is prepared into rich in life The fermentation egg-shell meal of object calcium, not only makes the calcium carbonate in eggshell obtain bioconversion, obtains the BIOLOGICAL CALCIUM for being conducive to animal absorption, And further converted to a large amount of protein, amino acid, carbohydrate is formed after saccharomycetes to make fermentation, make amino Acid, small peptide or small peptide small-molecule substance get a promotion, and more conducively animal is absorbed and utilized, and pass through what conversion carbohydrate was formed Inverted sugar and fragrance matter further improve the flavor and taste of product, increase animal to the preference of product.

（6）To the preparation of sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder in the present invention, do not remove the peel, deseeding step Suddenly, it is therefore an objective to by being crushed after freeze-drying, so that its skin and seed is worn into fine powder, make anthocyanidin, oil in its skin and seed Class, organic acid, bud fragrance substance are retained, and the nutritive value of its freeze-dried powder is promoted.

（7）The present invention is lyophilized using sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus Powder, matrix of the fermentation egg-shell meal as additive contain constants such as a large amount of calcium, phosphorus, potassium, sodium, magnesium, zinc and micro in matrix Element, vitamin C still rich in, anthocyanidin, beta carotene, malic acid, citric acid, protein, amino acid, nucleic acid, Portugal Grape sugar, lipoidis, carbohydrate, B family vitamin such as B₁、B₂、B₆、B₁₂And the physiological activity such as folic acid, niacin, pantothenic acid, inositol Substance, not only improves the nutritive value of enterococcus faecium additive, while promoting the metabolism of animal, improves the disease-resistant energy of body Power promotes growth of animal, finally improves the economic benefit of its aquaculture.

（8）Product of the present invention uses interior outer bag composite packaging, and interior outer bag interlayer is filled with silica-gel desiccant, and product does not return Tide, no agglomeration, product is loose, good fluidity, can preferably keep exterior quality and inherent quality.

Operation to not having its technical process stated in detail in the present invention is in the common sense of those skilled in the art Operation.With regard to the purchase and storage of heretofore described sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant fresh fruit and fresh cactus, the present invention The construction of people's unit has 20,000 tons of freezer facility, has enough guarantees to storage fresh fruit material.

Specific embodiment

Embodiment 1

The preparation of sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder：

Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the cactus for taking fresh mature respectively remove carpopodium, the impurity such as rotten fruit, with containing heavy 40 DEG C of aqueous solutions of 0.1% citric acid are measured, 15min is rinsed under constant stirring and pulls out, be put into pure water and rinse 3 times, pull out It is drained；It will be moved into refrigerator after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively, be cooled to -45 DEG C rapidly Below；Respectively by the sea-buckthorn freezed, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus move into lyophilization machine in, sublimation temperature control- 22--32 DEG C, vacuum degree 120-150Pa makes dry materials to moisture < 6%（w）, the time about needs 18 hours, is put into after taking-up In freezing crusher, control temperature of charge is no more than 0 DEG C, after being crushed to 100 mesh of fineness >, respectively obtains sea-buckthorn freeze-dried powder, thorn Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are fitted into polybag and seal, and are stored in 0 DEG C or less It is spare in freezer.

Embodiment 2

Using the culture medium accordingly optimized, candida utili, torrid zone vacation are individually cultivated using Liquid Culture Zymolysis Equipment Silk yeast, brewer's yeast, brewer's yeast and fodder yeast, are then separated the thallus in fermentation liquid with supercentrifuge, It is with vacuum freeze drying mode that bacterium mud is dry, respectively obtain candida utili dry powder that viable bacteria content is 200 hundred million/grams, Candida tropicalis dry powder that viable bacteria content is 200 hundred million/grams, the brewer's yeast dry powder that viable bacteria content is 200 hundred million/grams, work The feed yeast powder that the brewer's yeast dry powder and viable bacteria content that bacterial content is 200 hundred million/grams are 200 hundred million/grams.

Embodiment 3

The preparation of fermentation egg-shell meal：

One or more of mixing eggshells of egg shell or Ovum Anas domestica putamina, goose egg-shell, quail eggshell are cleaned up, moisture is dried, sets Enter in baking oven, under the conditions of 240 DEG C after heated-air drying 1.5 hours, taking-up is let cool, and is ground into 45 mesh powder；It is public to weigh egg-shell meal 80 Jin, suitable pure water, which is added, makes into material moisture 50%（W）, add the feed yeast powder 20 that embodiment 2 obtains Kilogram, rear sabot is mixed well, fermenting cellar is placed in, is fermented 60-72 hours under the conditions of temperature is 31-33 DEG C, to fermentation ends Afterwards, fermentation materials are adjusted to temperature is 82 DEG C -92 DEG C, under the conditions of temperature is 80-90%, carries out carbonyl ammonia and reacts 22-28 days, so Material is dried to moisture content < 5% under the conditions of being not more than 100 DEG C using electric baking case afterwards（W）, utilize omnipotent crushing Machine is crushed, and crosses 100 sieves to get fermentation egg-shell meal.

With the preparation method of the fermentation egg-shell meal of embodiment 3, it is dry that candida utili obtained in embodiment 2 can be respectively adopted Powder, candida tropicalis dry powder, brewer's yeast dry powder or brewer's yeast dry powder carry out preparation fermentation egg-shell meal, other process conditions Implemented according to eggshell powder preparation technique parameter of fermenting in the claim of this application 1-4.

Embodiment 4

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 30000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5g, Rosa roxburghii freeze-dried powder 5g, wintercherry freeze-drying Powder 3g, blackcurrant freeze-dried powder 3g, cactus freeze-dried powder 150g, remaining is fermentation egg-shell meal；Specifically production method is：Weigh implementation Sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mix example 1 respectively, Enterococcus faecium dry powder is uniformly admixed again, then add surplus ferment as made from embodiment 3 egg-shell meal be sufficiently mixed it is uniform after, Carry out composite packaging；Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, adds a few packet silicon between interior outer bag Glue desiccant, to prevent water suction.

Embodiment 5

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 55010 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 10g, wintercherry are frozen Dry powder 5g, blackcurrant freeze-dried powder 5g, cactus freeze-dried powder 85g, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.

Embodiment 6

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 75000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 15g, Rosa roxburghii freeze-dried powder 50g, wintercherry are frozen Dry powder 8g, blackcurrant freeze-dried powder 8g, cactus freeze-dried powder 90g, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.

Embodiment 7

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 90000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, wintercherry are frozen Dry powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 70g, remaining is fermentation egg-shell meal；The same embodiment of specific production method 4。

Embodiment 8

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 40g, Rosa roxburghii freeze-dried powder 30g, wintercherry are frozen Dry powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 70g, remaining is fermentation egg-shell meal；The same embodiment of specific production method 4。

Embodiment 9

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 130000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 10g, Rosa roxburghii freeze-dried powder 25g, wintercherry are frozen Dry powder 20g, blackcurrant freeze-dried powder 20g, cactus freeze-dried powder 80g, remaining is fermentation egg-shell meal；The same embodiment of specific production method 4。

Embodiment 10

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 100000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 40g, wintercherry are frozen Dry powder 30g, blackcurrant freeze-dried powder 30g, cactus freeze-dried powder 90g, remaining is fermentation egg-shell meal；The same embodiment of specific production method 4。

Embodiment 11

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 70000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 40g, wintercherry are frozen Dry powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 80g, remaining is fermentation egg-shell meal；The same embodiment of specific production method 4。

Embodiment 12

A kind of enterococcus faecium feed addictive, be by enterococcus faecium dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system At：The enterococcus faecium 120000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, wintercherry are frozen Dry powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 100g, remaining is fermentation egg-shell meal；Specific production method is the same as implementation Example 4.

Claims (10)

1. a kind of enterococcus faecium feed addictive, it is characterized in that first by enterococcus faecium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple Packaging is closed to be made：Enterococcus faecium 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is Ferment egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively, The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing condition described above is With the citric acid of 40 DEG C of water addition weight 0.1%, 15min is rinsed, and be stirred continuously, pulls out to be put into pure water and rinse 3 times；On Stating freezing is that will be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；Above-mentioned distillation Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；It is described Fermentation egg-shell meal, be to clean up eggshell, dry moisture, in advance be dried 1-2 hours, be then comminuted into 40-50 mesh Powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed thoroughly, in temperature It ferments 60-72 hours under the conditions of being 31 DEG C -33 DEG C, after fermentation, it is 82-92 DEG C, humidity that fermentation materials, which are adjusted to temperature, It is reacted 22-28 days to carry out carbonyl ammonia under the conditions of 80-90%, then, material is being not more than 100 DEG C of conditions using electric baking case Under be dried to moisture content < 5%/w, crushed, sieved with 100 mesh sieve to obtain the final product.

2. a kind of enterococcus faecium feed addictive, it is characterized in that first by enterococcus faecium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple Packaging is closed to be made：Enterococcus faecium 50000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 10- in every 1000g feed addictive 50g, Rosa roxburghii freeze-dried powder 10-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70-150g, Remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying Powder is made by following methods, and technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove fruit respectively Stalk, the impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing item described above Part is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pulls out to be put into pure water and rinses 3 It is secondary；Above-mentioned freezing is will to be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；It is above-mentioned The process conditions of lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w； The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours in advance, is then comminuted into 40- 50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed thoroughly, Temperature be 31 DEG C -33 DEG C under the conditions of ferment 60-72 hours, after fermentation, by fermentation materials be adjusted to temperature for 82-92 DEG C, Humidity carries out carbonyl ammonia and reacts 22-28 days under the conditions of being 80-90%, then, material is being not more than 100 DEG C using electric baking case Under the conditions of be dried to moisture content < 5%/w, crushed, sieved with 100 mesh sieve to obtain the final product.

3. a kind of enterococcus faecium feed addictive, it is characterized in that first by enterococcus faecium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple Packaging is closed to be made：Enterococcus faecium 70000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 15- in every 1000g feed addictive 50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85-150g, Remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying Powder is made by following methods, and technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove fruit respectively Stalk, the impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing item described above Part is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pulls out to be put into pure water and rinses 3 It is secondary；Above-mentioned freezing is will to be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；It is above-mentioned The process conditions of lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w； The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours in advance, is then comminuted into 40- 50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed thoroughly, Temperature be 31 DEG C -33 DEG C under the conditions of ferment 60-72 hours, after fermentation, by fermentation materials be adjusted to temperature for 82-92 DEG C, Humidity carries out carbonyl ammonia and reacts 22-28 days under the conditions of being 80-90%, then, material is being not more than 100 DEG C using electric baking case Under the conditions of be dried to moisture content < 5%/w, crushed, sieved with 100 mesh sieve to obtain the final product.

4. a kind of enterococcus faecium feed addictive, it is characterized in that first by enterococcus faecium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple Packaging is closed to be made：Enterococcus faecium 90000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 20- in every 1000g feed addictive 50g, Rosa roxburghii freeze-dried powder 20-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 100- 150g, remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being People slaps freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively The palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；It is described above Rinsing condition be to be rinsed 15min, and be stirred continuously, pulled out and be put into pure water with the citric acid of 40 DEG C of water addition weight 0.1% Middle rinsing 3 times；Above-mentioned freezing be will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively -45 DEG C with Under；The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, vacuum degree 120-150Pa, it is dry to moisture less than 6%/ Until w；The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours, then crushes in advance At 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed It is even, it ferments 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, it is 82- that fermentation materials, which are adjusted to temperature, 92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case be not more than It is dried to moisture content < 5%/w under the conditions of 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.

5. a kind of enterococcus faecium feed addictive as described in claim 1-4, which is characterized in that the enterococcus faecium is every 250 hundred million thalli dry powder products gram are no less than containing viable bacteria concentration.

6. a kind of enterococcus faecium feed addictive as described in claim 1-4, which is characterized in that the low-temperature grinding is material Temperature is in 0 DEG C or less and is crushed.

7. a kind of enterococcus faecium feed addictive as described in claim 1-4, which is characterized in that in the composite packaging uses Outer bag composite packaging, inner bag use polypropylene plastics pocket, and outer packing uses aluminium foil bag, is filled with silica-gel desiccant between interior outer bag Pouch.

8. a kind of enterococcus faecium feed addictive as described in claim 1-4, which is characterized in that the eggshell be egg shell, Any one or a few mixing eggshell in Ovum Anas domestica putamina, goose egg-shell and quail eggshell.

9. a kind of enterococcus faecium feed addictive as described in claim 1-4, which is characterized in that the drying uses hot air drying Dry or far-infrared ray drying；The drying condition is 180 DEG C -300 DEG C.

10. a kind of enterococcus faecium feed addictive as described in claim 1-4, which is characterized in that the saccharomycete is to produce protein Any one in Candida, candida tropicalis, brewer's yeast, brewer's yeast and fodder yeast.