CN108850483A - A kind of streptococcus fecalis feed addictive - Google Patents
A kind of streptococcus fecalis feed addictive Download PDFInfo
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- CN108850483A CN108850483A CN201810511935.4A CN201810511935A CN108850483A CN 108850483 A CN108850483 A CN 108850483A CN 201810511935 A CN201810511935 A CN 201810511935A CN 108850483 A CN108850483 A CN 108850483A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/225—Faecalis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A kind of streptococcus fecalis feed addictive of the present invention, it is characterized in that being uniformly mixed by streptococcus fecalis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, the fermentation egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made：Streptococcus fecalis 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is fermentation egg-shell meal.The present invention is single type streptococcus fecalis feed addictive, and clear containing viable count, has specific application and Quantitatively Selecting conducive to user.The present invention is using sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder and fermentation egg-shell meal as the matrix of additive, not only improve the nutritive value of streptococcus fecalis additive, promote the metabolism of animal simultaneously, improve the disease resistance of body, promote growth of animal, finally improves the economic benefit of its aquaculture.
Description
Technical field
The invention belongs to feed additive fields, are related to a kind of for cultivated animals probiotic feed additive.Specifically
It is related to a kind of streptococcus fecalis feed addictive.
Background technique
Streptococcus fecalis is living microorganism, can be acted on by the competitive exclusion of the alimentary canal biology of animal, inhibit to have
The growth of evil microbial bacteria forms dominant microflora or prevents disease by enhancing non-specific immune function, to promote dynamic
Object grows and improves food conversion ratio.
Streptococcus fecalis（streptococcus faecalis）It is that Food and Drug Administration and feed management association are recognized
To be safe microorganism fodder.With regard to the dosage form that current streptococcus fecalis uses as additive, there are aqua and pulvis two major classes,
Aqueous solution dosage form is because the nutritional substance in thallus aqua easy to use continues to be metabolized, growth fermentation, therefore, the bacterium of this dosage form
Body plays pendulum, and storage life is shorter.Powder formulation is then that the thallus lived is utilized the dry hand such as low temperature hot wind, freezing
Section, thallus is dehydrated at a slow speed, and thallus is made to be in stable drying dormant state, is recycled substance that can be feeding to be used as and is filled out
It fills material mixing and relatively stable powder-type biological feed additive is uniformly made.But raw material are considered with regard to current manufacturing enterprise
Be easy to get and processing cost, often select rice bran, wheat bran, corn flour, maize cob meal, peanut shell powder, conch meal, vermiculite power, mountain flour,
One or more of calcium carbonate, medical stone, zeolite powder mixture, some also use plant straw powder or grass meal as filling
Material.The considerations of these are in line with production cost then has ignored the stability of microbial bacteria and the trophism value of feed addictive.
In order in use, have certain adhesion, microbial bacteria feed addictive be made to exist in microbial bacteria additive
It admixes when being mixed in animal feed, can be adsorbed on animal feed, convenient for the uniformity in animal feeding and be fed into bacterium amount
Maximization, some products take pains a certain amount of binder of addition.
In order to make to raise the relative constant of animal stomach inner pH value, acid is continued up to after so that the foodstuff being fed into is entered small intestine
The effect of change, in the product often simultaneously using acidulants such as citric acid, lactic acid, hydrochloric acid, fumaric acid, but these acidulants are not only straight
It connects and locally causes acidity too strong microbial bacteria contained by additive, influence the survival of thallus, meanwhile, these acidifying substances are to cultivation
Animal also without the nutritive value directly utilized, meanwhile, there is very big irritation to the gastrointestinal tract of the cubs of livestock and poultry, chick,
Compatibility is using not ideal enough.
Above-mentioned deficiency can be overcome by developing one kind, can either keep stability, the trophism of institute's mycetome, be had again to thallus
There is adhesion, while having again to the filler used and providing the additive for microbe feedstuff of nutritive value to cultivated animals, is
Promote the basis of feed additive industry long-term stability development.
Summary of the invention
The purpose of the present invention is to provide a kind of new single type streptococcus fecalis feed addictive, which adds
Adding agent not only, there is thallus to stablize, and the thallus time-to-live is long, and used auxiliary agents have certain adhesiveness and suction
Attached property can make thallus preferably be bonding on one with auxiliary agents, meanwhile, complementary additive has certain acidity, right
After animal feeding, nutriment abundant can not only be provided, while to be able to maintain stomach inner pH value relative constant for its acidic materials, enters
The effect of acidification is continued up to after small intestine.The purpose is to provide the streptococcus fecalis feed addition of a kind of more stability and trophism
Agent.
A kind of streptococcus fecalis feed addictive of the present invention, it is characterized in that by streptococcus fecalis and a certain amount of seabuckthorn fruit powder, Rosa roxburghii Tratt
Powder, wintercherry powder, blackcurrant powder, cactus powder and fermentation egg-shell meal uniformly mix, and obtain a kind of new streptococcus fecalis feed addition
The thallus of agent, this additive is stablized, and the thallus time-to-live is long, and seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant powder sugar content
Height, especially cactus powder all have preferable Adhesion property, and fermentation egg-shell meal also has preferable adsorptivity.Wherein sea-buckthorn,
Rosa roxburghii Tratt, wintercherry, blackcurrant are the ascorbic fruits of high-content, and acidity is extremely strong, not only provide dimension abundant to cultivated animals
The nutriments such as element are given birth to, the acidic materials after feeding play the effect for promoting digestion well, to highlight the present invention
Purpose.
In order to realize the present invention, the following technical solutions are specifically adopted：
A kind of streptococcus fecalis feed addictive, feature it be first by streptococcus fecalis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：Streptococcus fecalis 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g,
Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is
Ferment egg-shell meal.
Streptococcus fecalis described above is streptococcus fecalis dry powder product.
The streptococcus fecalis dry powder product can be used and commercially available be used as every gram of feed addictive containing viable bacteria concentration
The weight proportion of no less than 250 hundred million preparations, the streptococcus fecalis dry powder product and the filler material in formula is done by thallus
The thallus content of powder and final products determines.
The streptococcus fecalis dry powder product also can use the culture medium that existing method selection accordingly optimizes and pass through liquid
Then Zymolysis Equipment culture is separated the thallus in fermentation liquid with high speed centrifugation mode, be placed in dry indoor cold wind or
Bacterium mud drying is made, every gram of viable bacteria concentration no less than 250 hundred million thalli dry powders of acquisition with vacuum freeze drying mode
Product.
Sea-buckthorn freeze-dried powder described above, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder,
It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively,
The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing condition described above is
With the citric acid of 40 DEG C of water addition weight 0.1%, 15min is rinsed, and be stirred continuously, pulls out to be put into pure water and rinse 3 times；On
Stating freezing is that will be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；Above-mentioned distillation
Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；It is above-mentioned
Low-temperature grinding is the crushing that temperature of charge is in 0 DEG C or less progress, 100 mesh of grinding particle size >.
Composite packaging described above uses interior outer bag composite packaging, and inner bag uses polypropylene plastics pocket, and outer packing uses aluminium
Foil bag is filled with silica-gel desiccant between interior outer bag.
Fermentation egg-shell meal described above, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, so
After be ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the ferment of egg-shell meal weight 20%
Female bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials is adjusted to temperature
Carbonyl ammonia is carried out under the conditions of being 80-90% for 82-92 DEG C, humidity（Also referred to as Maillard reaction）Reaction 22-28 days, then, by material
Moisture content < 5%/w is dried under the conditions of being not more than 100 DEG C using electric baking case, is crushed, is sieved with 100 mesh sieve to obtain the final product.
Drying described above uses heated-air drying or far-infrared ray drying；Dry condition is 180 DEG C -300 DEG C, drying
Purpose kill the harmful substances such as microorganism and helminth first is that reaching, second is that the crisp matterization of eggshell can be made to be conducive to crush, and make powder
Egg-shell meal after broken is loose conducive to fermentation.
The carbonyl ammonia reaction, i.e. Maillard reaction, also known as " non enzymatic browning reacton ", are carbonyls（Reduced sugar
Class）And amino-compound（Amino acid and protein）Between reaction.
The above-mentioned purpose that eggshell coarse powder is used to saccharomycetes to make fermentation, first is that can effectively make eggshell sufficiently soft using fermentation process
Change, the egg-shell meal after making fermentation is loose, is easy to mix with unclassified stores；Second is that can effectively be made in eggshell using saccharomycetes to make fermentation
The ingredients such as calcium obtain effective bioconversion, form BIOLOGICAL CALCIUM, conducive to being absorbed and utilized for animal, saccharomycetes to make fermentation can make fermentation material
Material forms the mass propagation of more protein, especially saccharomycete, and it is peculiar to increase yeast institute for the aggregation for the ingredient that can have additional nutrients
The multiple nutritional components such as glucose, protein, nucleic acid, vitamin, carbohydrate, lipid-like substances, especially B race dimension life
Element such as B1、B2、B6、B12And the physiological activators such as folic acid, niacin, pantothenic acid, inositol get a promotion, these beneficial nutrients
Matter ingredient undoubtedly plays the nutritional ingredient for improving product of the present invention, improves its nutritive validity.
The further carbonyl ammonia of egg-shell meal is reacted in the present invention, first is that promoting the maturing process of eggshell, is further made wherein
Calcareous ingredient obtain sufficient bioconversion, biological calcium component is formed, second is that obtaining the protein component in material sufficiently
Bioconversion, amino acid is degraded to, third is that a large amount of aminoacid ingredients to be formed is made to be converted into the small molecules object such as small peptide and small peptide
Matter, conducive to digesting and absorbing after animal feeding, the reaction of carbonyl ammonia not only promotes the trophic function of product, after the reaction of carbonyl ammonia
Material can further increase the application that gas porosity is conducive to mix feed, while carbohydrate being made to be converted into reduced sugar, produce simultaneously
Raw fragrance matter abundant, not only improves the anti-immunity ability of product, further improves the flavor of product, increase being fond of for animal
Property.
Eggshell described above is the mixed of any one or a few in egg shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell
Close eggshell.
Saccharomycete described above（saccharomyces）For candida utili（Candida utilis）, the torrid zone it is false
Silk yeast（Candida tropicalis）, brewer's yeast（Saccharomyces cerevisiae）, brewer's yeast
（Saccharomyces cerevisiae）And fodder yeast（Candida Tropicali）In any one.
Above-mentioned saccharomycete can choose using all viable bacterias as food additives or feed addictive commercially available
Concentration is no less than 200 every gram hundred million thalli dry powder products.
Existing method preparation can also be used in above-mentioned saccharomycete, such as can accordingly be optimized according to the selection of the property of strain
Then thallus in fermentation liquid is separated with high speed centrifugation mode, is set by the culture of Liquid Culture Zymolysis Equipment by culture medium
Bacterium mud drying is made in the indoor cold air drying of drying or with freeze-drying mode, obtaining viable bacteria concentration respectively is every gram
200 hundred million thalli dry powder products.
A kind of prioritization scheme of streptococcus fecalis feed addictive of the present invention is, first by streptococcus fecalis, sea-buckthorn freeze-dried powder, thorn
Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal of surplus
It is uniformly mixed, composite packaging is made：Streptococcus fecalis 50000 hundred million -150000 hundred million, sea-buckthorn in every 1000g feed addictive
Freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 10-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder
70-150g, remaining is fermentation egg-shell meal.
The further prioritization scheme of the present invention is a kind of streptococcus fecalis feed addictive, it is characterized in that first by excrement hammer
Bacterium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add
The fermentation egg-shell meal of surplus is uniformly mixed, and composite packaging is made：The streptococcus fecalis 70000 hundred million-in every 1000g feed addictive
150000 hundred million, sea-buckthorn freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-
30g, cactus freeze-dried powder 85-150g, remaining is fermentation egg-shell meal.
Further optimization plan of the present invention is a kind of streptococcus fecalis feed addictive, it is characterized in that first by excrement chain
Coccus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add
The fermentation egg-shell meal of surplus is added to be uniformly mixed, composite packaging is made：The streptococcus fecalis 90000 in every 1000g feed addictive
Hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 20-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder
10-30g, cactus freeze-dried powder 100-150g, remaining is fermentation egg-shell meal.
Sea-buckthorn（Hippophae rhamnoides Linn.）, fruit nutriment rich in and biological active matter
Matter can be widely applied to many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Fructus hippophae and oil
With very high medical value, there is a cough-relieving apophlegmatic, relieving dyspepsia, promoting blood circulation to remove blood stasis effect, can treat gastric duodenal ulcer with
And indigestion etc..
Sea buckthorn fruit is full of nutrition, according to surveying and determination in its fruit containing multivitamin, fatty acid, microelement, sub- oil element,
The various amino acid of Hippophate flavone, superoxides isoreactivity substance and needed by human body.Wherein Vitamin C content is high, and every 100 grams
In fruit juice, Vitamin C content can reach 825-1100 milligrams, is 2-3 times of Kiwi berry, is known as the laudatory title of the king of vitamin C.Contain
Sugared 7.5%-10%, the 3%-5% containing acid.
Rosa roxburghii Tratt（Rosa roxbunghii）For the fruit of the perennial machaka rosa roxburghii of rosaceae also known as mountain king fruit, thorn
Certain kind of berries fruit, thatch pears etc..With clearing heat and promoting fluid, stomach strengthening and digestion promoting, relieving summer-heat and other effects.Rosa roxburghii Tratt vitamin B rich in, C, carrotene,
The ingredients such as malic acid, citric acid are usually used in deficiency of stomach-Yin, appetite stimulator, indigestion or eating accumulation, glutted full bored, diarrhea
Loose stool；Pyreticosis or hot summer weather hurt saliva, thirsty, scanty drak urine.
Mature Rosa roxburghii Tratt is fleshy hypertrophy, sweet and sour, fruit are rich in sugar, vitamin, carrotene, organic acid and more than 20 kinds of ammonia
More than base acid, 10 microelement and superoxide dismutase, especially Vitamin C content beneficial to human body are high, are to work as
It is highest in preceding fruit, it is 50 times of citrus 841.58 milligrams -3541.13 milligrams of content in every 100 grams of fresh fruits, Kiwi berry
10 times, the laudatory title with " king of vitamin C ".Fruit of Grossularia burejensis Berger has very high nutritive value and medical value, sour, puckery, flat,
Digestion-promoting spleen-invigorating restrains stopping leak, for treating having indigestion abdominal distension, dysentery, enteritis, vitamin C deficiency etc..
Wintercherry（Physali alkekengiL.）Also known as groundcherry, franchet groundcherry persistent calyx or fruit, cape gooseberry etc..Containing more in Fruit of Physalis
Kind of nutritional ingredient, wherein the content of calcium be 73.1 times of tomato, 13.8 times of carrot, ascorbic content is tomato
6.4 times, 5.4 times of carrot.With unique flavor and nutrition abundant.Fruit of Physalis aromatic flavour, delicious.Berry is rich
Containing vitamin C, beta carotene, more than 20 kinds of mineral elements and 18 kinds of amino acid, there is heat-clearing, removing toxic substances, diuresis effect is usually used in
Control heat-syndrome cough, pharyngalgia, jaundice, dysentery, in water, the treatment of furunculosis, erysipelas etc..
Blackcurrant scientific name elements of black currant（Ribes nigrum L.）Also known as currant, purple plum etc., ripening fruits are black
Small berries, it is interior to be rich in vitamin C, anthocyanidin.Blackcurrant is not only a kind of delicious flavour, fruit full of nutrition, and has
Good medicinal efficacy.Blackcurrant fruit is rich in mineral matter element, and the Zn content in mineral matter element is high, can be used as and comes for zinc
Source；Ascorbic content is high, hence it is evident that is higher than citrus, hawthorn, also contains phosphorus, magnesium, potassium, calcium, anthocyanidin, phenolic substances.Seed
Linoleic acid content is high.Ascorbic content is very rich in blackcurrant, it is often more important that is also melted into containing a large amount of antioxygen
Point, this assures ascorbic function and effect.Blackcurrant is rich in such as anthocyanidin, vitamin C, flavones, Quercetin, red bayberry
The substances such as alcohol, phenolic acid, catechin and blackcurrant polysaccharide, these are all the bioactive substances with good anti-oxidation function.
Cactus（Scientific name：Opuntia stricta(Haw.)Haw.var.dillenii(Ker-Gawl.) Benson）,
It is a kind of plant of Opuntia.Alias celestial being palm, kwan-yin palm etc..Cactus is the meat shrub that grows thickly, the wide ovum of top branch
Shape, obovate elliptic or subcircular；Flower spoke shape, holder obovate.It is used as medicine with complete stool.The four seasons can adopt.
Cactus is cool in nature, sour, nontoxic.Have the promoting flow of qi and blood circulation, heat-clearing toxin-expelling functions.Mainly have clearing heat and detoxicating, eliminating stasis to subdue swelling,
The functions such as the promoting flow of qi and blood circulation, benefiting stomach and stopping pain.Cactus contains amino acid and various trace elements in needed by human 8, and embraces green
The preciousness ingredient such as lotus, Kok Beattie cents, cholla stem, starches through cactus crude drug and analyzes, contain pectin, polysaccharide and glue exudate.From herb point
From extracting to obtain freidelin ketone（friedelin）, -3 α -ol of freidelin（friedelan-3-α-ol）, taraxerone
（taraxerone）And taraxerol（taraxerol）.
Eggshell, quantity is biggish in China's poultry farming chicken, duck, goose and quail etc., using egg, duck's egg,
As in food processing process, the useless eggshell enormous amount generated is provided according to existing disclosed report for goose egg and quail egg
Material display, the eggshell total amount that a medium-sized city is monthly discarded reach 60-100 tons, and account for egg size by Eggshell weight 11% calculates,
The eggshell that about 1,500,000 tons of the daily output in China, not only pollutes environment, but also waste of resource in this way.Discarded eggshell is integrated
It utilizes, resource utilization can not only be improved and avoids environmental pollution, and be greatly improved the economic benefit and social benefit of enterprise.
Wherein egg shell is in China's yield maximum, and egg shell accounts for the 10-12% in entire egg, by under film, shell on shell
Film and eggshell three parts composition, eggshell is a kind of calcareous hard shell, is located on shell between film and shell lower film.In the change of egg shell
In studying point, minerals account for 95%, and protein 3.3%, moisture 1%, fat 0.03%, remaining is carbohydrate and other have
Machine substance（It certainly, does not include a large amount of remaining egg white in eggshell in the above ingredient）.Eggshell mineral ingredient is mainly
Calcium carbonate accounts for 94% or so, remaining is magnesium carbonate and calcium phosphate, and calcium, phosphorus content account for 34.9% and of eggshell dry matter respectively
0.2%.In eggshell, seven kinds of vitamins and various trace elements and some important enzymes, peptide matters can be measured, he
Although content seldom, be all the substance needed in human body or animal body.
In the prior art, it is replenished the calcium with egg-shell meal, has the product of the grinds after sterilizing by eggshell in people's product, also have
The application of organic calcium is prepared into using primary or secondary clacining method.It is at present most widely in drug, food and nutriment
In preparation, micron order, submicron order and nano level superfine powder are mostly used, is added to various food, nutrition kind, has reached very
Good effect.Application of the eggshell in China's animal-breeding is taken mostly by eggshell cleaning, drying, and milling is added into feed
The method of feeding, since grinding particle size is larger, being absorbed and utilized for animal is poor, can't reach good application effect.
The present invention is first to be ground into coarse powder by after eggshell cleaning, high temperature drying, fermented using saccharomycete, then again
Carbonyl ammonia reaction treatment is carried out, then dried, crush and be prepared into biological calcium product, the program not only improves what eggshell utilized
Nutrition, while the main composition-calcium carbonate in eggshell being made to obtain biochemical reaction, the biological calcium carbonate for being more conducive to absorb is formed,
The reaction of carbonyl ammonia makes its protein component be further converted to amino acid again, makes the protein, amino acid converting for small peptide of macromolecular
Or the small-molecule substances such as small peptide, the beneficial effect for reaching collection eutrophy and being absorbed and utilized.
The beneficial effects of the invention are as follows：
（1）Product of the present invention can highlight the viable bacteria content of streptococcus fecalis, significantly improve thallus depositing in closed composite packaging
Motility rate, through batches of products reserved sample observing 12 months at room temperature, streptococcus fecalis average viability is 93.7%, and reference substance
（The filler of reference substance is wheat bran）In streptococcus fecalis survival rate be 78.2%；Product of the present invention is the feeding of single type streptococcus fecalis
Feed additives can provide the selection of the specific aim and quantization with application to producer, be conducive to targeted to livestock and poultry
It is convenient to use.
（2）Pass through sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder and excrement
Streptococcus mixes, and due to the stickum in the colloidal substance that these dry powder formulations are rich in, especially cactus, can preferably increase
Add the adhesive effect between additive and feed, and improves the utilization rate of additive；
（3）By sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder high-content amino acid, vitamin, mineral element, more
Sugar.The nutritional ingredients such as the vitamin C of organic acid, especially high-content and various bioactivators, to raising animal immune energy
Power is simultaneously obviously improved meat, Egg Quality, the acidic materials in especially above-mentioned dry powder, utilization, promotion animal to contained microorganism
Digestion all has significant effect；
（4）Due to using Freeze Drying Technique means, enable sea-buckthorn obtained, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder
Its active constituent is maximumlly remained, has distinctive sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the smell of cactus application and taste
Taste, free from extraneous odour add the preference of animal.
（5）Using egg-shell meal first through saccharomycetes to make fermentation, carbonyl ammonia is then used（Maillard reaction）Reaction is prepared into rich in life
The fermentation egg-shell meal of object calcium, not only makes the calcium carbonate in eggshell obtain bioconversion, obtains the BIOLOGICAL CALCIUM for being conducive to animal absorption,
And further converted to a large amount of protein, amino acid, carbohydrate is formed after saccharomycetes to make fermentation, make amino
Acid, small peptide or small peptide small-molecule substance get a promotion, and more conducively animal is absorbed and utilized, and pass through what conversion carbohydrate was formed
Inverted sugar and fragrance matter further improve the flavor and taste of product, increase animal to the preference of product.
（6）To the preparation of sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder in the present invention, do not remove the peel, deseeding step
Suddenly, it is therefore an objective to by being crushed after freeze-drying, so that its skin and seed is worn into fine powder, make anthocyanidin, oil in its skin and seed
Class, organic acid, bud fragrance substance are retained, and the nutritive value of its freeze-dried powder is promoted.
（7）The present invention is lyophilized using sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus
Powder, matrix of the fermentation egg-shell meal as additive contain constants such as a large amount of calcium, phosphorus, potassium, sodium, magnesium, zinc and micro in matrix
Element, vitamin C still rich in, anthocyanidin, beta carotene, malic acid, citric acid, protein, amino acid, nucleic acid, Portugal
Grape sugar, lipoidis, carbohydrate, B family vitamin such as B1、B2、B6、B12And the physiological activity such as folic acid, niacin, pantothenic acid, inositol
Substance, not only improves the nutritive value of streptococcus fecalis additive, while promoting the metabolism of animal, improves the disease-resistant energy of body
Power promotes growth of animal, finally improves the economic benefit of its aquaculture.
（8）Product of the present invention uses interior outer bag composite packaging, and interior outer bag interlayer is filled with silica-gel desiccant, and product does not return
Tide, no agglomeration, product is loose, good fluidity, can preferably keep exterior quality and inherent quality.
Operation to not having its technical process stated in detail in the present invention is in the common sense of those skilled in the art
Operation.With regard to the purchase and storage of heretofore described sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant fresh fruit and fresh cactus, the present invention
The construction of people's unit has 20,000 tons of freezer facility, has enough guarantees to storage fresh fruit material.
Specific embodiment
Embodiment 1
The preparation of sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder：
Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the cactus for taking fresh mature respectively remove carpopodium, the impurity such as rotten fruit, with containing heavy
40 DEG C of aqueous solutions of 0.1% citric acid are measured, 15min is rinsed under constant stirring and pulls out, be put into pure water and rinse 3 times, pull out
It is drained；It will be moved into refrigerator after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively, be cooled to -45 DEG C rapidly
Below；Respectively by the sea-buckthorn freezed, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus move into lyophilization machine in, sublimation temperature control-
22--32 DEG C, vacuum degree 120-150Pa makes dry materials to moisture < 6%（w）, the time about needs 18 hours, is put into after taking-up
In freezing crusher, control temperature of charge is no more than 0 DEG C, after being crushed to 100 mesh of fineness >, respectively obtains sea-buckthorn freeze-dried powder, thorn
Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are fitted into polybag and seal, and are stored in 0 DEG C or less
It is spare in freezer.
Embodiment 2
Using the culture medium accordingly optimized, candida utili, torrid zone vacation are individually cultivated using Liquid Culture Zymolysis Equipment
Silk yeast, brewer's yeast, brewer's yeast and fodder yeast, are then separated the thallus in fermentation liquid with supercentrifuge,
It is with vacuum freeze drying mode that bacterium mud is dry, respectively obtain candida utili dry powder that viable bacteria content is 200 hundred million/grams,
Candida tropicalis dry powder that viable bacteria content is 200 hundred million/grams, the brewer's yeast dry powder that viable bacteria content is 200 hundred million/grams, work
The feed yeast powder that the brewer's yeast dry powder and viable bacteria content that bacterial content is 200 hundred million/grams are 200 hundred million/grams.
Embodiment 3
The preparation of fermentation egg-shell meal：
One or more of mixing eggshells of egg shell or Ovum Anas domestica putamina, goose egg-shell, quail eggshell are cleaned up, moisture is dried, sets
Enter in baking oven, under the conditions of 240 DEG C after heated-air drying 1.5 hours, taking-up is let cool, and is ground into 45 mesh powder；It is public to weigh egg-shell meal 80
Jin, suitable pure water, which is added, makes into material moisture 50%（W）, add the feed yeast powder 20 that embodiment 2 obtains
Kilogram, rear sabot is mixed well, fermenting cellar is placed in, is fermented 60-72 hours under the conditions of temperature is 31-33 DEG C, to fermentation ends
Afterwards, fermentation materials are adjusted to temperature is 82 DEG C -92 DEG C, under the conditions of temperature is 80-90%, carries out carbonyl ammonia and reacts 22-28 days, so
Material is dried to moisture content < 5% under the conditions of being not more than 100 DEG C using electric baking case afterwards（W）, utilize omnipotent crushing
Machine is crushed, and crosses 100 sieves to get fermentation egg-shell meal.
With the preparation method of the fermentation egg-shell meal of embodiment 3, it is dry that candida utili obtained in embodiment 2 can be respectively adopted
Powder, candida tropicalis dry powder, brewer's yeast dry powder or brewer's yeast dry powder carry out preparation fermentation egg-shell meal, other process conditions
Implemented according to eggshell powder preparation technique parameter of fermenting in the claim of this application 1-4.
Embodiment 4
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 30000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5g, Rosa roxburghii freeze-dried powder 5g, wintercherry freeze-drying
Powder 3g, blackcurrant freeze-dried powder 3g, cactus freeze-dried powder 150g, remaining is fermentation egg-shell meal；Specifically production method is：Weigh implementation
Sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mix example 1 respectively,
Streptococcus fecalis dry powder is uniformly admixed again, then add surplus ferment as made from embodiment 3 egg-shell meal be sufficiently mixed it is uniform after,
Carry out composite packaging；Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, adds a few packet silicon between interior outer bag
Glue desiccant, to prevent water suction.
Embodiment 5
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 50000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 10g, wintercherry are frozen
Dry powder 5g, blackcurrant freeze-dried powder 5g, cactus freeze-dried powder 85g, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 6
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 70000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 15g, Rosa roxburghii freeze-dried powder 50g, wintercherry are frozen
Dry powder 8g, blackcurrant freeze-dried powder 8g, cactus freeze-dried powder 90g, remaining is fermentation egg-shell meal；Specific production method is the same as embodiment 4.
Embodiment 7
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 90000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, wintercherry are frozen
Dry powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 70g, remaining is fermentation egg-shell meal；The same embodiment of specific production method
4。
Embodiment 8
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 10g, Rosa roxburghii freeze-dried powder 30g, wintercherry are frozen
Dry powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 75g, remaining is fermentation egg-shell meal；The same embodiment of specific production method
4。
Embodiment 9
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 130000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 40g, Rosa roxburghii freeze-dried powder 25g, wintercherry are frozen
Dry powder 20g, blackcurrant freeze-dried powder 20g, cactus freeze-dried powder 80g, remaining is fermentation egg-shell meal；The same embodiment of specific production method
4。
Embodiment 10
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 100000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 40g, wintercherry are frozen
Dry powder 30g, blackcurrant freeze-dried powder 30g, cactus freeze-dried powder 90g, remaining is fermentation egg-shell meal；The same embodiment of specific production method
4。
Embodiment 11
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 110000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 40g, wintercherry are frozen
Dry powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 80g, remaining is fermentation egg-shell meal；The same embodiment of specific production method
4。
Embodiment 12
A kind of streptococcus fecalis feed addictive, be by streptococcus fecalis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder,
Blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging system
At：The streptococcus fecalis 120000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, wintercherry are frozen
Dry powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 100g, remaining is fermentation egg-shell meal；Specific production method is the same as implementation
Example 4.
Claims (10)
1. a kind of streptococcus fecalis feed addictive, it is characterized in that first by streptococcus fecalis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：Streptococcus fecalis 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g,
Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is
Ferment egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder,
It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively,
The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing condition described above is
With the citric acid of 40 DEG C of water addition weight 0.1%, 15min is rinsed, and be stirred continuously, pulls out to be put into pure water and rinse 3 times；On
Stating freezing is that will be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；Above-mentioned distillation
Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；It is described
Fermentation egg-shell meal, be to clean up eggshell, dry moisture, in advance be dried 1-2 hours, be then comminuted into 40-50 mesh
Powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed thoroughly, in temperature
It ferments 60-72 hours under the conditions of being 31 DEG C -33 DEG C, after fermentation, it is 82-92 DEG C, humidity that fermentation materials, which are adjusted to temperature,
It is reacted 22-28 days to carry out carbonyl ammonia under the conditions of 80-90%, then, material is being not more than 100 DEG C of conditions using electric baking case
Under be dried to moisture content < 5%/w, crushed, sieved with 100 mesh sieve to obtain the final product.
2. a kind of streptococcus fecalis feed addictive, it is characterized in that first by streptococcus fecalis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：Streptococcus fecalis 50000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 10- in every 1000g feed addictive
50g, Rosa roxburghii freeze-dried powder 10-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70-150g,
Remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying
Powder is made by following methods, and technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove fruit respectively
Stalk, the impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing item described above
Part is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pulls out to be put into pure water and rinses 3
It is secondary；Above-mentioned freezing is will to be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；It is above-mentioned
The process conditions of lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；
The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours in advance, is then comminuted into 40-
50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed thoroughly,
Temperature be 31 DEG C -33 DEG C under the conditions of ferment 60-72 hours, after fermentation, by fermentation materials be adjusted to temperature for 82-92 DEG C,
Humidity carries out carbonyl ammonia and reacts 22-28 days under the conditions of being 80-90%, then, material is being not more than 100 DEG C using electric baking case
Under the conditions of be dried to moisture content < 5%/w, crushed, sieved with 100 mesh sieve to obtain the final product.
3. a kind of streptococcus fecalis feed addictive, it is characterized in that first by streptococcus fecalis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：Streptococcus fecalis 70000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 15- in every 1000g feed addictive
50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85-150g,
Remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying
Powder is made by following methods, and technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove fruit respectively
Stalk, the impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；Rinsing item described above
Part is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pulls out to be put into pure water and rinses 3
It is secondary；Above-mentioned freezing is will to be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；It is above-mentioned
The process conditions of lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；
The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours in advance, is then comminuted into 40-
50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed thoroughly,
Temperature be 31 DEG C -33 DEG C under the conditions of ferment 60-72 hours, after fermentation, by fermentation materials be adjusted to temperature for 82-92 DEG C,
Humidity carries out carbonyl ammonia and reacts 22-28 days under the conditions of being 80-90%, then, material is being not more than 100 DEG C using electric baking case
Under the conditions of be dried to moisture content < 5%/w, crushed, sieved with 100 mesh sieve to obtain the final product.
4. a kind of streptococcus fecalis feed addictive, it is characterized in that first by streptococcus fecalis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid
It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the fermentation egg-shell meal of surplus and be uniformly mixed, it is multiple
Packaging is closed to be made：Streptococcus fecalis 90000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 20- in every 1000g feed addictive
50g, Rosa roxburghii freeze-dried powder 20-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 100-
150g, remaining is fermentation egg-shell meal；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being
People slaps freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively
The palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step；It is described above
Rinsing condition be to be rinsed 15min, and be stirred continuously, pulled out and be put into pure water with the citric acid of 40 DEG C of water addition weight 0.1%
Middle rinsing 3 times；Above-mentioned freezing be will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively -45 DEG C with
Under；The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, vacuum degree 120-150Pa, it is dry to moisture less than 6%/
Until w；The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours, then crushes in advance
At 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed
It is even, it ferments 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, it is 82- that fermentation materials, which are adjusted to temperature,
92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case be not more than
It is dried to moisture content < 5%/w under the conditions of 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
5. a kind of streptococcus fecalis feed addictive as described in claim 1-4, which is characterized in that the streptococcus fecalis is every
250 hundred million thalli dry powder products gram are no less than containing viable bacteria concentration.
6. a kind of streptococcus fecalis feed addictive as described in claim 1-4, which is characterized in that the low-temperature grinding is material
Temperature is in 0 DEG C or less and is crushed.
7. a kind of streptococcus fecalis feed addictive as described in claim 1-4, which is characterized in that in the composite packaging uses
Outer bag composite packaging, inner bag use polypropylene plastics pocket, and outer packing uses aluminium foil bag, is filled with silica-gel desiccant between interior outer bag
Pouch.
8. a kind of streptococcus fecalis feed addictive as described in claim 1-4, which is characterized in that the eggshell be egg shell,
Any one or a few mixing eggshell in Ovum Anas domestica putamina, goose egg-shell and quail eggshell.
9. a kind of streptococcus fecalis feed addictive as described in claim 1-4, which is characterized in that the drying uses hot air drying
Dry or far-infrared ray drying；The drying condition is 180 DEG C -300 DEG C.
10. a kind of streptococcus fecalis feed addictive as described in claim 1-4, which is characterized in that the saccharomycete is to produce protein
Any one in Candida, candida tropicalis, brewer's yeast, brewer's yeast and fodder yeast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810511935.4A CN108850483A (en) | 2018-05-25 | 2018-05-25 | A kind of streptococcus fecalis feed addictive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810511935.4A CN108850483A (en) | 2018-05-25 | 2018-05-25 | A kind of streptococcus fecalis feed addictive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108850483A true CN108850483A (en) | 2018-11-23 |
Family
ID=64333278
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810511935.4A Withdrawn CN108850483A (en) | 2018-05-25 | 2018-05-25 | A kind of streptococcus fecalis feed addictive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108850483A (en) |
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2018
- 2018-05-25 CN CN201810511935.4A patent/CN108850483A/en not_active Withdrawn
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