CN108850465A - A kind of lactobacillus plantarum feed addictive - Google Patents

A kind of lactobacillus plantarum feed addictive Download PDF

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Publication number
CN108850465A
CN108850465A CN201810511866.7A CN201810511866A CN108850465A CN 108850465 A CN108850465 A CN 108850465A CN 201810511866 A CN201810511866 A CN 201810511866A CN 108850465 A CN108850465 A CN 108850465A
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China
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freeze
dried powder
powder
lactobacillus plantarum
sea
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张奎昌
张志年
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Jiangsu Qianyaotang Traditional Chinese Medicine Research Institute Co Ltd
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Jiangsu Qianyaotang Traditional Chinese Medicine Research Institute Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum

Abstract

A kind of lactobacillus plantarum feed addictive of the present invention, it is characterized in that being uniformly mixed by lactobacillus plantarum, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, the fermentation egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made:Lactobacillus plantarum 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is fermentation egg-shell meal.The present invention is single type lactobacillus plantarum feed addictive, and clear containing viable count, has specific application and Quantitatively Selecting conducive to user.The present invention is using sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder and fermentation egg-shell meal as the matrix of additive, not only improve the nutritive value of lactobacillus plantarum additive, promote the metabolism of animal simultaneously, improve the disease resistance of body, promote growth of animal, finally improves the economic benefit of its aquaculture.

Description

A kind of lactobacillus plantarum feed addictive
Technical field
The invention belongs to feed additive fields, are related to a kind of for cultivated animals probiotic feed additive.Specifically It is related to a kind of lactobacillus plantarum feed addictive.
Background technique
Lactic acid bacteria(Lactic acid bacteria, LAB)The lactic acid bacteria being currently known has more than 100 kind.Lactic acid bacteria Applicating history is very long, such as the production and production of dairy products, pickles, ensilage.In the enteron aisle of humans and animals there is A large amount of lactic acid bacteria.Lactobacillus preparation is a kind of preparation containing live lactobacillus, entitled alctasin.Lactic acid bacteria grows in enteron aisle Development decomposes carbohydrate and generates lactic acid and form acidic environment, to inhibit the proliferation of harmful bacteria in intestinal canal, and prevent from producing poison and It causes a disease.The strain for preparing lactobacillus preparation has lactobacillus acidophilus, lactobacillus bulgaricus, streptococcus lactis and streptococcus fecalis etc..
Lactobacillus is animal(Especially poultry) enteron aisle normal settler.Poultry went out after shell in a few hours can be Streptococcus fecalis, the enterobacteria that discovery is dispersed in enteron aisle() and clostridium Enterobateria(Clostridia), with Bacillus acidi lactici just starts to settle down in alimentary canal in a couple of days afterwards.Lactobacillus is the main Pseudomonas of fowl piperazine capsule flora, with large intestine Bacillus, streptococcus(Streptococi it) compares and occupies advantage.Bacillus acidi lactici is adhered to fowl prolixity capsular epithelium, and this adhesion has kind Specificity.Fowl piperazine capsule Bacillus acidi lactici is the Bacillus acidi lactici source that poultry maintains intestinal bacteria balance.Bacillus acidi lactici is maintaining to digest The aspect of stablizing of the normal microflora system in road plays conclusive effect.Most probiotic products be Lactobacillus or other Lactic acid-producing bacteria.
Lactic acid bacteria anaerobism or amphimicrobian growth, still are able to survive under pH3.0-4.5 acid condition.It is in animal intestinal tract Middle by monosaccharide, especially Lactose conversion is lactic acid, to reduce intestinal pH, prevents foreign bacteria in the field planting of enteron aisle, inhibits large intestine The growth of the pathogens such as bacillus, detection of Salmonella, and the growth of final lactic acid bacteria inhibiting itself.Lactic acid bacteria also can be by adjusting enteron aisle PH promotes gastrointestinal peristalsis to activate pepsin, helps the digestion of food, absorbs, mitigate flatulence and promote liver function etc.. Lactic acid bacteria is acted on by competitive exclusion(Competitive binding site) inhibit pathogen growth.Lactic acid bacteria can also stimulate siberian crabapple System improves immunity of organisms, improves intestinal tissue to the resistivity of bacteria attack.Some Bacillus acidi lacticis can neutralize certain toxin, As lactobacillus bulgaricus can neutralize escherichia coli enterotoxin, but precise mechanism is unclear.Bacillus acidi lactici also can produce itself Some digestive ferments, such as invertase, lactase and peptase, help to improve animal digestion function.Sawada report, certain lactic acid Contain a kind of safe and effective natural anti-hypertension compound SG-1 in bacilli-cell wall.Lang Zhongwu is equal to 1994 in egg young bird Brood of layers chichen is added in daily grain of chicken, as a result feed efficiency improves 8.73%-11.23%;Xiao Zhenfeng etc.(1995) it tries Verify bright, Brood of layers chichen is compared compared with antibiotic control group, improves feed efficiency 4.6%.Furthermore Bacillus acidi lactici can The digestive function of small intestine is improved by increasing surface area.Animal is in stress(As birth, wean, daily ration variation, intestinal pH are abnormal It increases)Period, total variation tendency are that pathogen is increased, and Bacillus acidi lactici quantity is reduced.Lactobacillus is successfully established in enteron aisle PH can be made to become stable.
The characteristics of lactic acid bacteria is:1. being the main fungal component of many animals alimentary canal, normal flora can be formed:2. in micro- need Lactic acid is generated under oxygen or anaerobic condition;3. there is stronger acid resistance;4. generating a kind of bacteriocin
(Bacteriocin), hay bacillus, staphylococcic growth can effectively be inhibited.The shortcomings that lactic acid bacteria is that temperature capacity is poor, Dead under the conditions of 65 ~ 75 DEG C, instantaneous high-temperature can be killed and be failed during feed granules.The type of preparation of such bacterium It is various, using earliest, most extensively.
Lactobacillus plantarum(lacbobacillus plantarum)For Food and Drug Administration and feed management association It is considered safe microorganism fodder.There is relatively broad application in aquaculture industry of China at present.With regard to current lactobacillus plantarum conduct The dosage form that additive uses has aqua and pulvis two major classes, and aqueous solution dosage form is because of the trophism in thallus aqua easy to use Substance continues to be metabolized, and growth fermentation, therefore, the thallus of this dosage form plays pendulum, and storage life is shorter.Powder formulation is then It is that thallus is dehydrated at a slow speed the thallus lived to using drying means such as low temperature hot wind, freezings, so that thallus is in stable and do Dry dormant state recycles substance that can be feeding to be uniformly mixed as filler and relatively stable powder-type biology feeding is made Feed additives.But with regard to current manufacturing enterprise in view of raw material be easy to get and processing cost, often select rice bran, wheat bran, corn flour, The mixing of one or more of maize cob meal, peanut shell powder, conch meal, vermiculite power, mountain flour, calcium carbonate, medical stone, zeolite powder Object, some also use plant straw powder or grass meal as filler material.The considerations of these are in line with production cost, then have ignored microorganism The stability of bacterium and the trophism value of feed addictive.
In order in use, have certain adhesion, microbial bacteria feed addictive be made to exist in microbial bacteria additive It admixes when being mixed in animal feed, can be adsorbed on animal feed, convenient for the uniformity in animal feeding and be fed into bacterium amount Maximization, some products take pains a certain amount of binder of addition.
In order to make to raise the relative constant of animal stomach inner pH value, acid is continued up to after so that the foodstuff being fed into is entered small intestine The effect of change, in the product often simultaneously using acidulants such as citric acid, lactic acid, hydrochloric acid, fumaric acid, but these acidulants are not only straight It connects and locally causes acidity too strong microbial bacteria contained by additive, influence the survival of thallus, meanwhile, these acidifying substances are to cultivation Animal also without the nutritive value directly utilized, meanwhile, there is very big irritation to the gastrointestinal tract of the cubs of livestock and poultry, chick, Compatibility is using not ideal enough.
Above-mentioned deficiency can be overcome by developing one kind, can either keep stability, the trophism of institute's mycetome, be had again to thallus There is adhesion, while having again to the filler used and providing the additive for microbe feedstuff of nutritive value to cultivated animals, is Promote the basis of feed additive industry long-term stability development.
Summary of the invention
The purpose of the present invention is to provide a kind of new single type lactobacillus plantarum feed addictive, which is raised Not only there is feed additives thallus to stablize, and the thallus time-to-live is long, and used auxiliary agents have certain adhesiveness And adsorptivity, thallus can be made preferably to be bonding on one with auxiliary agents, meanwhile, complementary additive has certain acid Property can not only provide nutriment abundant after animal feeding, while to be able to maintain stomach inner pH value relatively permanent for its acidic materials It is fixed, into small intestine after the effect of continuing up to acidification.The purpose is to provide the plumule cream bar of a kind of more stability and trophism Bacterium feed addictive.
A kind of lactobacillus plantarum feed addictive of the present invention, it is characterized in that by lactobacillus plantarum and a certain amount of seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant powder, cactus powder and fermentation egg-shell meal uniformly mix, and obtain a kind of new lactobacillus plantarum feeding The thallus of feed additives, this additive is stablized, and the thallus time-to-live is long, and seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant powder Sugar content is high, and especially cactus powder all has preferable Adhesion property, and fermentation egg-shell meal also has preferable adsorptivity.Wherein Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant are the ascorbic fruits of high-content, and acidity is extremely strong, are not only provided to cultivated animals rich The nutriments such as rich vitamin, the acidic materials after feeding play the effect for promoting digestion well, to highlight The purpose of the present invention.
In order to realize the present invention, the following technical solutions are specifically adopted:
A kind of lactobacillus plantarum feed addictive, feature it be first by lactobacillus plantarum, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt be lyophilized Powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus Even, composite packaging is made:Lactobacillus plantarum 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-drying Powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50- 150g, remaining is fermentation egg-shell meal.
Lactobacillus plantarum described above is lactobacillus plantarum dry powder product.
The lactobacillus plantarum dry powder product, can be used it is commercially available be used as every gram of feed addictive it is dense containing viable bacteria The weight proportion of the preparation that degree is no less than 250 hundred million, the lactobacillus plantarum dry powder product and the filler material in formula is by bacterium The thallus content of soma powder and final products determines.
The lactobacillus plantarum dry powder product also can use the culture medium that existing method selection accordingly optimizes and pass through liquid Then body Zymolysis Equipment culture is separated the thallus in fermentation liquid with high speed centrifugation mode, be placed in dry indoor cold wind Or bacterium mud drying is made with vacuum freeze drying mode, every gram of viable bacteria concentration no less than 250 hundred million thallus of acquisition are dry Powder product.
Sea-buckthorn freeze-dried powder described above, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively, The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;Rinsing condition described above is With the citric acid of 40 DEG C of water addition weight 0.1%, 15min is rinsed, and be stirred continuously, pulls out to be put into pure water and rinse 3 times;On Stating freezing is that will be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively;Above-mentioned distillation Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w;It is above-mentioned Low-temperature grinding is the crushing that temperature of charge is in 0 DEG C or less progress, 100 mesh of grinding particle size >.
Composite packaging described above uses interior outer bag composite packaging, and inner bag uses polypropylene plastics pocket, and outer packing uses aluminium Foil bag is filled with silica-gel desiccant between interior outer bag.
Fermentation egg-shell meal described above, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, so After be ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the ferment of egg-shell meal weight 20% Female bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials is adjusted to temperature Carbonyl ammonia is carried out under the conditions of being 80-90% for 82-92 DEG C, humidity(Also referred to as Maillard reaction)Reaction 22-28 days, then, by material Moisture content < 5%/w is dried under the conditions of being not more than 100 DEG C using electric baking case, is crushed, is sieved with 100 mesh sieve to obtain the final product.
Drying described above uses heated-air drying or far-infrared ray drying;Dry condition is 180 DEG C -300 DEG C, drying Purpose kill the harmful substances such as microorganism and helminth first is that reaching, second is that the crisp matterization of eggshell can be made to be conducive to crush, and make powder Egg-shell meal after broken is loose conducive to fermentation.
The carbonyl ammonia reaction, i.e. Maillard reaction, also known as " non enzymatic browning reacton ", are carbonyls(Reduced sugar Class)And amino-compound(Amino acid and protein)Between reaction.
The above-mentioned purpose that eggshell coarse powder is used to saccharomycetes to make fermentation, first is that can effectively make eggshell sufficiently soft using fermentation process Change, the egg-shell meal after making fermentation is loose, is easy to mix with unclassified stores;Second is that can effectively be made in eggshell using saccharomycetes to make fermentation The ingredients such as calcium obtain effective bioconversion, form BIOLOGICAL CALCIUM, conducive to being absorbed and utilized for animal, saccharomycetes to make fermentation can make fermentation material Material forms the mass propagation of more protein, especially saccharomycete, and it is peculiar to increase yeast institute for the aggregation for the ingredient that can have additional nutrients The multiple nutritional components such as glucose, protein, nucleic acid, vitamin, carbohydrate, lipid-like substances, especially B race dimension life Element such as B1、B2、B6、B12And the physiological activators such as folic acid, niacin, pantothenic acid, inositol get a promotion, these beneficial nutrients Matter ingredient undoubtedly plays the nutritional ingredient for improving product of the present invention, improves its nutritive validity.
The further carbonyl ammonia of egg-shell meal is reacted in the present invention, first is that promoting the maturing process of eggshell, is further made wherein Calcareous ingredient obtain sufficient bioconversion, biological calcium component is formed, second is that obtaining the protein component in material sufficiently Bioconversion, amino acid is degraded to, third is that a large amount of aminoacid ingredients to be formed is made to be converted into the small molecules object such as small peptide and small peptide Matter, conducive to digesting and absorbing after animal feeding, the reaction of carbonyl ammonia not only promotes the trophic function of product, after the reaction of carbonyl ammonia Material can further increase the application that gas porosity is conducive to mix feed, while carbohydrate being made to be converted into reduced sugar, produce simultaneously Raw fragrance matter abundant, not only improves the anti-immunity ability of product, further improves the flavor of product, increase being fond of for animal Property.
Eggshell described above is the mixed of any one or a few in egg shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell Close eggshell.
Saccharomycete described above(saccharomyces)For candida utili(Candida utilis), the torrid zone it is false Silk yeast(Candida tropicalis), brewer's yeast(Saccharomyces cerevisiae), brewer's yeast (Saccharomyces cerevisiae)And fodder yeast(Candida Tropicali)In any one.
Above-mentioned saccharomycete can choose using all viable bacterias as food additives or feed addictive commercially available Concentration is no less than 200 every gram hundred million thalli dry powder products.
Existing method preparation can also be used in above-mentioned saccharomycete, such as can accordingly be optimized according to the selection of the property of strain Then thallus in fermentation liquid is separated with high speed centrifugation mode, is set by the culture of Liquid Culture Zymolysis Equipment by culture medium Bacterium mud drying is made in the indoor cold air drying of drying or with freeze-drying mode, obtaining viable bacteria concentration respectively is every gram 200 hundred million thalli dry powder products.
A kind of prioritization scheme of lactobacillus plantarum feed addictive of the present invention is to be lyophilized first by lactobacillus plantarum, sea-buckthorn Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermented egg of surplus Shell powder is uniformly mixed, and composite packaging is made:Lactobacillus plantarum 50000 hundred million -150000 in every 1000g feed addictive Hundred million, sea-buckthorn freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 10-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus Freeze-dried powder 70-150g, remaining is fermentation egg-shell meal.
The further prioritization scheme of the present invention is a kind of lactobacillus plantarum feed addictive, it is characterized in that first by plumule Lactobacillus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then The fermentation egg-shell meal for adding surplus is uniformly mixed, and composite packaging is made:The lactobacillus plantarum in every 1000g feed addictive 70000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 8-30g, blackcurrant Freeze-dried powder 8-30g, cactus freeze-dried powder 85-150g, remaining is fermentation egg-shell meal.
Further optimization plan of the present invention is a kind of lactobacillus plantarum feed addictive, it is characterized in that first by embryo Bud lactobacillus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, The fermentation egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made:The lactobacillus plantarum in every 1000g feed addictive 90000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 20-50g, wintercherry freeze-dried powder 10-30g, it is black plus Logical sequence freeze-dried powder 10-30g, cactus freeze-dried powder 100-150g, remaining is fermentation egg-shell meal.
Sea-buckthorn(Hippophae rhamnoides Linn.), fruit nutriment rich in and biological active matter Matter can be widely applied to many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Fructus hippophae and oil With very high medical value, there is a cough-relieving apophlegmatic, relieving dyspepsia, promoting blood circulation to remove blood stasis effect, can treat gastric duodenal ulcer with And indigestion etc..
Sea buckthorn fruit is full of nutrition, according to surveying and determination in its fruit containing multivitamin, fatty acid, microelement, sub- oil element, The various amino acid of Hippophate flavone, superoxides isoreactivity substance and needed by human body.Wherein Vitamin C content is high, and every 100 grams In fruit juice, Vitamin C content can reach 825-1100 milligrams, is 2-3 times of Kiwi berry, is known as the laudatory title of the king of vitamin C.Contain Sugared 7.5%-10%, the 3%-5% containing acid.
Rosa roxburghii Tratt(Rosa roxbunghii)For the fruit of the perennial machaka rosa roxburghii of rosaceae also known as mountain king fruit, thorn Certain kind of berries fruit, thatch pears etc..With clearing heat and promoting fluid, stomach strengthening and digestion promoting, relieving summer-heat and other effects.Rosa roxburghii Tratt vitamin B rich in, C, carrotene, The ingredients such as malic acid, citric acid are usually used in deficiency of stomach-Yin, appetite stimulator, indigestion or eating accumulation, glutted full bored, diarrhea Loose stool;Pyreticosis or hot summer weather hurt saliva, thirsty, scanty drak urine.
Mature Rosa roxburghii Tratt is fleshy hypertrophy, sweet and sour, fruit are rich in sugar, vitamin, carrotene, organic acid and more than 20 kinds of ammonia More than base acid, 10 microelement and superoxide dismutase, especially Vitamin C content beneficial to human body are high, are to work as It is highest in preceding fruit, it is 50 times of citrus 841.58 milligrams -3541.13 milligrams of content in every 100 grams of fresh fruits, Kiwi berry 10 times, the laudatory title with " king of vitamin C ".Fruit of Grossularia burejensis Berger has very high nutritive value and medical value, sour, puckery, flat, Digestion-promoting spleen-invigorating restrains stopping leak, for treating having indigestion abdominal distension, dysentery, enteritis, vitamin C deficiency etc..
Wintercherry(Physali alkekengiL.)Also known as groundcherry, franchet groundcherry persistent calyx or fruit, cape gooseberry etc..Containing more in Fruit of Physalis Kind of nutritional ingredient, wherein the content of calcium be 73.1 times of tomato, 13.8 times of carrot, ascorbic content is tomato 6.4 times, 5.4 times of carrot.With unique flavor and nutrition abundant.Fruit of Physalis aromatic flavour, delicious.Berry is rich Containing vitamin C, beta carotene, more than 20 kinds of mineral elements and 18 kinds of amino acid, there is heat-clearing, removing toxic substances, diuresis effect is usually used in Control heat-syndrome cough, pharyngalgia, jaundice, dysentery, in water, the treatment of furunculosis, erysipelas etc..
Blackcurrant scientific name elements of black currant(Ribes nigrum L.)Also known as currant, purple plum etc., ripening fruits are black Small berries, it is interior to be rich in vitamin C, anthocyanidin.Blackcurrant is not only a kind of delicious flavour, fruit full of nutrition, and has Good medicinal efficacy.Blackcurrant fruit is rich in mineral matter element, and the Zn content in mineral matter element is high, can be used as and comes for zinc Source;Ascorbic content is high, hence it is evident that is higher than citrus, hawthorn, also contains phosphorus, magnesium, potassium, calcium, anthocyanidin, phenolic substances.Seed Linoleic acid content is high.Ascorbic content is very rich in blackcurrant, it is often more important that is also melted into containing a large amount of antioxygen Point, this assures ascorbic function and effect.Blackcurrant is rich in such as anthocyanidin, vitamin C, flavones, Quercetin, red bayberry The substances such as alcohol, phenolic acid, catechin and blackcurrant polysaccharide, these are all the bioactive substances with good anti-oxidation function.
Cactus(Scientific name:Opuntia stricta(Haw.)Haw.var.dillenii(Ker-Gawl.) Benson), It is a kind of plant of Opuntia.Alias celestial being palm, kwan-yin palm etc..Cactus is the meat shrub that grows thickly, the wide ovum of top branch Shape, obovate elliptic or subcircular;Flower spoke shape, holder obovate.It is used as medicine with complete stool.The four seasons can adopt.
Cactus is cool in nature, sour, nontoxic.Have the promoting flow of qi and blood circulation, heat-clearing toxin-expelling functions.Mainly have clearing heat and detoxicating, eliminating stasis to subdue swelling, The functions such as the promoting flow of qi and blood circulation, benefiting stomach and stopping pain.Cactus contains amino acid and various trace elements in needed by human 8, and embraces green The preciousness ingredient such as lotus, Kok Beattie cents, cholla stem, starches through cactus crude drug and analyzes, contain pectin, polysaccharide and glue exudate.From herb point From extracting to obtain freidelin ketone(friedelin), -3 α -ol of freidelin(friedelan-3-α-ol), taraxerone (taraxerone)And taraxerol(taraxerol).
Eggshell, quantity is biggish in China's poultry farming chicken, duck, goose and quail etc., using egg, duck's egg, As in food processing process, the useless eggshell enormous amount generated is provided according to existing disclosed report for goose egg and quail egg Material display, the eggshell total amount that a medium-sized city is monthly discarded reach 60-100 tons, and account for egg size by Eggshell weight 11% calculates, The eggshell that about 1,500,000 tons of the daily output in China, not only pollutes environment, but also waste of resource in this way.Discarded eggshell is integrated It utilizes, resource utilization can not only be improved and avoids environmental pollution, and be greatly improved the economic benefit and social benefit of enterprise.
Wherein egg shell is in China's yield maximum, and egg shell accounts for the 10-12% in entire egg, by under film, shell on shell Film and eggshell three parts composition, eggshell is a kind of calcareous hard shell, is located on shell between film and shell lower film.In the change of egg shell In studying point, minerals account for 95%, and protein 3.3%, moisture 1%, fat 0.03%, remaining is carbohydrate and other have Machine substance(It certainly, does not include a large amount of remaining egg white in eggshell in the above ingredient).Eggshell mineral ingredient is mainly Calcium carbonate accounts for 94% or so, remaining is magnesium carbonate and calcium phosphate, and calcium, phosphorus content account for 34.9% and of eggshell dry matter respectively 0.2%.In eggshell, seven kinds of vitamins and various trace elements and some important enzymes, peptide matters can be measured, he Although content seldom, be all the substance needed in human body or animal body.
In the prior art, it is replenished the calcium with egg-shell meal, has the product of the grinds after sterilizing by eggshell in people's product, also have The application of organic calcium is prepared into using primary or secondary clacining method.It is at present most widely in drug, food and nutriment In preparation, micron order, submicron order and nano level superfine powder are mostly used, is added to various food, nutrition kind, has reached very Good effect.Application of the eggshell in China's animal-breeding is taken mostly by eggshell cleaning, drying, and milling is added into feed The method of feeding, since grinding particle size is larger, being absorbed and utilized for animal is poor, can't reach good application effect.
The present invention is first to be ground into coarse powder by after eggshell cleaning, high temperature drying, fermented using saccharomycete, then again Carbonyl ammonia reaction treatment is carried out, then dried, crush and be prepared into biological calcium product, the program not only improves what eggshell utilized Nutrition, while the main composition-calcium carbonate in eggshell being made to obtain biochemical reaction, the biological calcium carbonate for being more conducive to absorb is formed, The reaction of carbonyl ammonia makes its protein component be further converted to amino acid again, makes the protein, amino acid converting for small peptide of macromolecular Or the small-molecule substances such as small peptide, the beneficial effect for reaching collection eutrophy and being absorbed and utilized.
The beneficial effects of the invention are as follows:
(1)Product of the present invention can highlight the viable bacteria content of lactobacillus plantarum, significantly improve thallus in closed composite packaging Survival rate, through batches of products reserved sample observing 12 months at room temperature, lactobacillus plantarum average viability is and right 93.7% According to product(The filler of reference substance is wheat bran)In lactobacillus plantarum survival rate be 78.2%;Product of the present invention is single type plumule Lactobacillus feed addictive can provide the selection of the specific aim and quantization with application to producer, have conducive to livestock and poultry Targetedly it is easy to use.
(2)Pass through sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder and embryo Bud lactobacillus mixes, can be preferably due to the stickum in the colloidal substance that these dry powder formulations are rich in, especially cactus The adhesive effect between additive and feed is increased, and improves the utilization rate of additive;
(3)By sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder high-content amino acid, vitamin, mineral element, more Sugar.The nutritional ingredients such as the vitamin C of organic acid, especially high-content and various bioactivators, to raising animal immune energy Power is simultaneously obviously improved meat, Egg Quality, the acidic materials in especially above-mentioned dry powder, utilization, promotion animal to contained microorganism Digestion all has significant effect;
(4)Due to using Freeze Drying Technique means, enable sea-buckthorn obtained, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder Its active constituent is maximumlly remained, has distinctive sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the smell of cactus application and taste Taste, free from extraneous odour add the preference of animal.
(5)Using egg-shell meal first through saccharomycetes to make fermentation, carbonyl ammonia is then used(Maillard reaction)Reaction is prepared into rich in life The fermentation egg-shell meal of object calcium, not only makes the calcium carbonate in eggshell obtain bioconversion, obtains the BIOLOGICAL CALCIUM for being conducive to animal absorption, And further converted to a large amount of protein, amino acid, carbohydrate is formed after saccharomycetes to make fermentation, make amino Acid, small peptide or small peptide small-molecule substance get a promotion, and more conducively animal is absorbed and utilized, and pass through what conversion carbohydrate was formed Inverted sugar and fragrance matter further improve the flavor and taste of product, increase animal to the preference of product.
(6)To the preparation of sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder in the present invention, do not remove the peel, deseeding step Suddenly, it is therefore an objective to by being crushed after freeze-drying, so that its skin and seed is worn into fine powder, make anthocyanidin, oil in its skin and seed Class, organic acid, bud fragrance substance are retained, and the nutritive value of its freeze-dried powder is promoted.
(7)The present invention is lyophilized using sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus Powder, matrix of the fermentation egg-shell meal as additive contain constants such as a large amount of calcium, phosphorus, potassium, sodium, magnesium, zinc and micro in matrix Element, vitamin C still rich in, anthocyanidin, beta carotene, malic acid, citric acid, protein, amino acid, nucleic acid, Portugal Grape sugar, lipoidis, carbohydrate, B family vitamin such as B1、B2、B6、B12And the physiological activity such as folic acid, niacin, pantothenic acid, inositol Substance, not only improves the nutritive value of lactobacillus plantarum additive, while promoting the metabolism of animal, improves the disease-resistant of body Ability promotes growth of animal, finally improves the economic benefit of its aquaculture.
(8)Product of the present invention uses interior outer bag composite packaging, and interior outer bag interlayer is filled with silica-gel desiccant, and product does not return Tide, no agglomeration, product is loose, good fluidity, can preferably keep exterior quality and inherent quality.
Operation to not having its technical process stated in detail in the present invention is in the common sense of those skilled in the art Operation.With regard to the purchase and storage of heretofore described sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant fresh fruit and fresh cactus, the present invention The construction of people's unit has 20,000 tons of freezer facility, has enough guarantees to storage fresh fruit material.
Specific embodiment
Embodiment 1
The preparation of sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder:
Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the cactus for taking fresh mature respectively remove carpopodium, the impurity such as rotten fruit, with containing heavy 40 DEG C of aqueous solutions of 0.1% citric acid are measured, 15min is rinsed under constant stirring and pulls out, be put into pure water and rinse 3 times, pull out It is drained;It will be moved into refrigerator after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively, be cooled to -45 DEG C rapidly Below;Respectively by the sea-buckthorn freezed, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus move into lyophilization machine in, sublimation temperature control- 22--32 DEG C, vacuum degree 120-150Pa makes dry materials to moisture < 6%(w), the time about needs 18 hours, is put into after taking-up In freezing crusher, control temperature of charge is no more than 0 DEG C, after being crushed to 100 mesh of fineness >, respectively obtains sea-buckthorn freeze-dried powder, thorn Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are fitted into polybag and seal, and are stored in 0 DEG C or less It is spare in freezer.
Embodiment 2
Using the culture medium accordingly optimized, candida utili, torrid zone vacation are individually cultivated using Liquid Culture Zymolysis Equipment Silk yeast, brewer's yeast, brewer's yeast and fodder yeast, are then separated the thallus in fermentation liquid with supercentrifuge, It is with vacuum freeze drying mode that bacterium mud is dry, respectively obtain candida utili dry powder that viable bacteria content is 200 hundred million/grams, Candida tropicalis dry powder that viable bacteria content is 200 hundred million/grams, the brewer's yeast dry powder that viable bacteria content is 200 hundred million/grams, work The feed yeast powder that the brewer's yeast dry powder and viable bacteria content that bacterial content is 200 hundred million/grams are 200 hundred million/grams.
Embodiment 3
The preparation of fermentation egg-shell meal:
One or more of mixing eggshells of egg shell or Ovum Anas domestica putamina, goose egg-shell, quail eggshell are cleaned up, moisture is dried, sets Enter in baking oven, under the conditions of 240 DEG C after heated-air drying 1.5 hours, taking-up is let cool, and is ground into 45 mesh powder;It is public to weigh egg-shell meal 80 Jin, suitable pure water, which is added, makes into material moisture 50%(W), add the feed yeast powder 20 that embodiment 2 obtains Kilogram, rear sabot is mixed well, fermenting cellar is placed in, is fermented 60-72 hours under the conditions of temperature is 31-33 DEG C, to fermentation ends Afterwards, fermentation materials are adjusted to temperature is 82 DEG C -92 DEG C, under the conditions of temperature is 80-90%, carries out carbonyl ammonia and reacts 22-28 days, so Material is dried to moisture content < 5% under the conditions of being not more than 100 DEG C using electric baking case afterwards(W), utilize omnipotent crushing Machine is crushed, and crosses 100 sieves to get fermentation egg-shell meal.
With the preparation method of the fermentation egg-shell meal of embodiment 3, it is dry that candida utili obtained in embodiment 2 can be respectively adopted Powder, candida tropicalis dry powder, brewer's yeast dry powder or brewer's yeast dry powder carry out preparation fermentation egg-shell meal, other process conditions Implemented according to eggshell powder preparation technique parameter of fermenting in the claim of this application 1-4.
Embodiment 4
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 30000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5g, Rosa roxburghii freeze-dried powder 5g, acid Freeze-dried powder 3g, blackcurrant freeze-dried powder 3g, cactus freeze-dried powder 150g are starched, remaining is fermentation egg-shell meal;Specifically production method is:Claim The difference of Example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder It mixes, then lactobacillus plantarum dry powder is uniformly admixed, then add the surplus egg-shell meal that ferments as made from embodiment 3 and be sufficiently mixed After uniformly, composite packaging is carried out;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, between interior outer bag Add a few packet silica-gel desiccants, to prevent water suction.
Embodiment 5
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 50000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 10g, Wintercherry freeze-dried powder 5g, blackcurrant freeze-dried powder 5g, cactus freeze-dried powder 85g, remaining is fermentation egg-shell meal;Specific production method is the same as real Apply example 4.
Embodiment 6
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 70000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 15g, Rosa roxburghii freeze-dried powder 50g, Wintercherry freeze-dried powder 8g, blackcurrant freeze-dried powder 8g, cactus freeze-dried powder 90g, remaining is fermentation egg-shell meal;Specific production method is the same as real Apply example 4.
Embodiment 7
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 90000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, Wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 70g, remaining is fermentation egg-shell meal;Specific production method is same Embodiment 4.
Embodiment 8
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 10g, Rosa roxburghii freeze-dried powder 30g, Wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 75g, remaining is fermentation egg-shell meal;Specific production method is same Embodiment 4.
Embodiment 9
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 130000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 40g, Rosa roxburghii freeze-dried powder 25g, Wintercherry freeze-dried powder 20g, blackcurrant freeze-dried powder 20g, cactus freeze-dried powder 80g, remaining is fermentation egg-shell meal;Specific production method is same Embodiment 4.
Embodiment 10
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 100000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 40g, Wintercherry freeze-dried powder 30g, blackcurrant freeze-dried powder 30g, cactus freeze-dried powder 90g, remaining is fermentation egg-shell meal;Specific production method is same Embodiment 4.
Embodiment 11
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 110000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 40g, Wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 80g, remaining is fermentation egg-shell meal;Specific production method is same Embodiment 4.
Embodiment 12
A kind of lactobacillus plantarum feed addictive is frozen by lactobacillus plantarum dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus fermentation egg-shell meal be uniformly mixed, compound packet Dress is made:The lactobacillus plantarum 120000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, Wintercherry freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 100g, remaining is fermentation egg-shell meal;Specific production method With embodiment 4.

Claims (10)

1. a kind of lactobacillus plantarum feed addictive, it is characterized in that being lyophilized first by lactobacillus plantarum, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus Even, composite packaging is made:Lactobacillus plantarum 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-drying Powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50- 150g, remaining is fermentation egg-shell meal;The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being People slaps freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively The palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;It is described above Rinsing condition be to be rinsed 15min, and be stirred continuously, pulled out and be put into pure water with the citric acid of 40 DEG C of water addition weight 0.1% Middle rinsing 3 times;Above-mentioned freezing be will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively -45 DEG C with Under;The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, vacuum degree 120-150Pa, it is dry to moisture less than 6%/ Until w;The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours, then crushes in advance At 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed It is even, it ferments 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, it is 82- that fermentation materials, which are adjusted to temperature, 92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case be not more than It is dried to moisture content < 5%/w under the conditions of 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
2. a kind of lactobacillus plantarum feed addictive, it is characterized in that being lyophilized first by lactobacillus plantarum, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus Even, composite packaging is made:Lactobacillus plantarum 50000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-drying Powder 10-50g, Rosa roxburghii freeze-dried powder 10-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70- 150g, remaining is fermentation egg-shell meal;The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being People slaps freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively The palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;It is described above Rinsing condition be to be rinsed 15min, and be stirred continuously, pulled out and be put into pure water with the citric acid of 40 DEG C of water addition weight 0.1% Middle rinsing 3 times;Above-mentioned freezing be will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively -45 DEG C with Under;The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, vacuum degree 120-150Pa, it is dry to moisture less than 6%/ Until w;The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours, then crushes in advance At 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed It is even, it ferments 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, it is 82- that fermentation materials, which are adjusted to temperature, 92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case be not more than It is dried to moisture content < 5%/w under the conditions of 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
3. a kind of lactobacillus plantarum feed addictive, it is characterized in that being lyophilized first by lactobacillus plantarum, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus Even, composite packaging is made:Lactobacillus plantarum 70000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-drying Powder 15-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85- 150g, remaining is fermentation egg-shell meal;The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being People slaps freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively The palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;It is described above Rinsing condition be to be rinsed 15min, and be stirred continuously, pulled out and be put into pure water with the citric acid of 40 DEG C of water addition weight 0.1% Middle rinsing 3 times;Above-mentioned freezing be will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively -45 DEG C with Under;The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, vacuum degree 120-150Pa, it is dry to moisture less than 6%/ Until w;The fermentation egg-shell meal, is to clean up eggshell, dries moisture, is dried 1-2 hours, then crushes in advance At 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, and the saccharomycete for adding egg-shell meal weight 20% is mixed It is even, it ferments 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, it is 82- that fermentation materials, which are adjusted to temperature, 92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case be not more than It is dried to moisture content < 5%/w under the conditions of 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
4. a kind of lactobacillus plantarum feed addictive, it is characterized in that being lyophilized first by lactobacillus plantarum, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation egg-shell meal mixing of surplus Even, composite packaging is made:Lactobacillus plantarum 90000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-drying Powder 20-50g, Rosa roxburghii freeze-dried powder 20-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 100-150g, remaining is fermentation egg-shell meal;The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-drying Powder, cactus freeze-dried powder, are made by following methods, technical process be take respectively fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, Cactus removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;It is above-mentioned The rinsing condition is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pull out be put into it is pure It is rinsed 3 times in water purification;Above-mentioned freezing is will to be cooled to rapidly -45 after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively DEG C or less;The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dried to moisture and is less than Until 6%/w;The fermentation egg-shell meal, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, then It is ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the yeast of egg-shell meal weight 20% Bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials, which are adjusted to temperature, is 82-92 DEG C, humidity be 80-90% under the conditions of carry out carbonyl ammonia react 22-28 days, then, by material use electric baking case not It is dried to moisture content < 5%/w under the conditions of greater than 100 DEG C, is crushed, sieved with 100 mesh sieve to obtain the final product.
5. a kind of lactobacillus plantarum feed addictive as described in claim 1-4, which is characterized in that the lactobacillus plantarum, 250 hundred million thalli dry powder products are no less than containing viable bacteria concentration for every gram.
6. a kind of lactobacillus plantarum feed addictive as described in claim 1-4, which is characterized in that the low-temperature grinding is object Material temperature degree is in 0 DEG C or less and is crushed.
7. a kind of lactobacillus plantarum feed addictive as described in claim 1-4, which is characterized in that the composite packaging uses Interior outer bag composite packaging, inner bag use polypropylene plastics pocket, and outer packing uses aluminium foil bag, is filled with silica dehydrator between interior outer bag Agent pouch.
8. a kind of lactobacillus plantarum feed addictive as described in claim 1-4, which is characterized in that the eggshell is egg Any one or a few mixing eggshell in shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell.
9. a kind of lactobacillus plantarum feed addictive as described in claim 1-4, which is characterized in that the drying uses hot wind Dry or far-infrared ray drying;The drying condition is 180 DEG C -300 DEG C.
10. a kind of lactobacillus plantarum feed addictive as described in claim 1-4, which is characterized in that the saccharomycete is to produce Any one in protein Candida, candida tropicalis, brewer's yeast, brewer's yeast and fodder yeast.
CN201810511866.7A 2018-05-25 2018-05-25 A kind of lactobacillus plantarum feed addictive Withdrawn CN108850465A (en)

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