CN101664215A - Compound beverage containing probiotics and collagen and preparation method thereof - Google Patents
Compound beverage containing probiotics and collagen and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of foods and particularly relates to a compound beverage containing probiotics and collagen and a preparation method thereof. In order to solve the problemthat the probiotics in the existing probiotics beverage is difficult to store for long period, the probiotics and the collagen are separately packaged in the invention and then combined to form the compound beverage containing probiotics and collagen. The compound beverage includes a solid part and a liquid part, wherein the solid part at least includes the probiotics, and the liquid part includes a fixed component and an unfixed component; the fixed component is the collagen; and the solid part also comprises an auxiliary carrier used for prolonging the survival time of probiotics. The technical proposal can prolong the survival time of probiotics by at least twice or more.
Description
Technical field
The invention belongs to food technology field, be specifically related to a kind of composite beverage that contains probio and collagen and preparation method thereof.
Background technology
The probio collagen oral liquid is a kind of composite beverage, and is main by additional human collagen albumen and probio, and makes human body more healthy.Collagen is by the synthetic a kind of boiomacromolecule of zooblast, is a kind of white, opaque, unbranched fibrous type protein, and it has the function that supports organ and protection human body, also is the most important functions albumen of forming cytoplasm.Collagen is with its natural hypotoxicity, low antigenicity, hypoimmunity, the migration that can suppress the epithelial tissue cell, energy cell guiding regeneration and good with human compatibility.The fibre structure of collagen uniqueness and excellent biological compatibility thereof and low antigenicity make it become the cosmetics of super quality, the popular research topic of functional health-care food and field of biomedical materials.Can replenish the required nutrient of skin layer, replenish 17 kinds of amino acid to the human body beneficial, the integrality that keeps cuticula moisture and fibre structure, improve the metabolism that the Skin Cell living environment promotes skin histology, strengthen circulation, reach skin care, delay senility, beauty treatment, wrinkle-chasing, the purpose of hair care.
The traditional beverage or the preparation method of oral liquid are by the utility in the raw material is extracted, join other raw materials again, add an amount of pure water, be made into liquid or liquid and solid Combination material, can is in vial, in plastic bottle, pottery bottle or the metal can, sealing, sterilization.Also there are preceding material of can and packing container to sterilize respectively.The collagen beverage major part also is a liquid, in the probiotic products of liquid the time-to-live of probio very short, through after a while preservation or transportation, active probiotic quantity sharply descends, often when the consumer drank probiotic beverage, the probio in the probiotic beverage was dead substantially.
Summary of the invention
Technical problem to be solved by this invention is that the probio in the existing probiotic beverage is difficult to the problem of long preservation, and a kind of composite beverage that contains probio and collagen and preparation method thereof is provided.
The present invention solves the problems of the technologies described above by following technical solution:
A kind of composite beverage that contains probio and collagen, comprise solid portion and liquid part, described solid portion comprises probio at least, described liquid partly comprises fixedly component and on-fixed component, described fixedly component is a collagen, described solid portion also comprises the assistant carrier that is used to prolong the probio survival period, and the probio that above-mentioned solid portion comprises at least can be the probiotics bacterial powder, also can be probiotic microcapsule particle etc.
The present invention uses the problem that two kinds of probios in the means solution probiotic beverage are difficult to long preservation: first, use the solid mode to preserve probio, the preservation of microorganism need be created an environmental condition that is fit to its long-term dormancy, such as low temperature, drying, anoxic, lucifuge, lack nutrition etc., make microorganism minimizing metabolic rate and breeding that is saved and the ratio that utilizes nutrition, the solid mode is preserved probio and be can be probio and create above-mentioned dormancy condition, thereby increases substantially the holding time of probio; Second, it is 50～99.5% the assistant carrier that is used to prolong the probio survival period that solid portion comprises mass ratio (W/W), adopt this assistant carrier can significantly improve holding time of probio, the assistant carrier that is used for prolonging the probio survival period of the present invention comprises the mixture of a kind of, two kinds or the multiple composition of powdered glucose, maltodextrin, cane sugar powder, lactose powder, water-soluble dietary fiber, converted starch, resistant starch, corn flour, rice meal, wheat flour etc.
On the basis of technique scheme, composite beverage of the present invention also replenishes the collagen of needed by human body by two kinds of approach, first kind of approach is external direct absorption collagen, the present invention has added collagen in the liquid part of composite beverage, the consumer can be directly by drinking composite beverage replenishing collagen of the present invention; Second kind of approach is synthetic collagen in the body, the present invention finds, hydroxylation step during collagen synthesizes in vitamin and the human body has certain relation, when hypovitaminosis or deficiency, can influence the synthetic of collagen, it is 0.01～10% functional component that a kind of composite beverage that contains probio and collagen of the present invention has increased mass ratio (W/W) at the solid portion of described composite beverage thus, and this functional component is vitamin A, B
1, B
2, B
6, B
12, C, D
3, among the E one or more, thereby can promote the synthetic of collagen in the body; Two kinds of approach can guarantee that beverage of the present invention is to the human body replenishing collagen thus.
Probio of the present invention can be selected for use in principle and anyly improve the human body microecological balance and bring into play beneficial effect, reaches the active bacteria formulation and the metabolite thereof that improve human health level and health status.For guaranteeing that probio can long preservation, probio of the present invention is a Bacillus acidi lactici, saccharomycete, Bifidobacterium, two or more any compatibility in the streptococcus thermophilus, wherein Bacillus acidi lactici is a lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, described Bifidobacterium is a bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, these bacterial classifications all are the good purebred of typical bacterial classification, survival rate height not only, and can be through keeping higher biologically active after the long preservation.
Should be noted that, the present invention does not make restriction to each bacterial classification input amount ratio, but as a design of the present invention, the present invention's suggestion is when those skilled in the art select Bacillus acidi lactici and streptococcus thermophilus as probio, preferably with streptococcus thermophilus and Bacillus acidi lactici Mixed culture, its reason is Bacillus acidi lactici energy decomposing protein, accumulation amino acid and organic acid, amino acid and organic acid can promote the growth of streptococcus thermophilus, and streptococcus thermophilus can produce Cobastab, promote the growth of Bacillus acidi lactici, if with both Mixed culture, the comparable difference of its effect is incubation growth good alone.The input ratio of streptococcus thermophilus and Bacillus acidi lactici mixed lactobacillus and streptococcus thermophilus is 1: 1.
The viable count of the probio of composite beverage of the present invention is generally 10
6～10
9The cfu/ gram.
For the nutrition that makes composite beverage of the present invention more complete, special taste, composite beverage of the present invention comprises that gross mass volume ratio (W/V) is 1.0～25% liquid part, and the on-fixed component of wherein said liquid part is two or more any compatibility in chondroitin, extract of soybean, kudzu root extract, condensed water fruit juice, fructose, HFCS, corn steep liquor, citric acid, malic acid, the essence.Wherein kudzu root extract or extract of soybean can increase bone density, replenish class estrogen, improve skin, and chondroitin can keep moisture content in the body, promote bone health, lubricated joint.
Can realize that for the composite beverage that the present invention is designed the present invention also provides a kind of method for preparing composite beverage of the present invention, this method comprises solid portion preparation method and liquid part preparation method, and described solid portion preparation method comprises:
1. strain fermentation:
Adopt following bacterial strain: lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, streptococcus thermophilus, saccharomyces cerevisiae, through single strain fermentation or mixing fermentation culture, obtain zymocyte liquid, fermentation is controlled at 4～8 with the initial pH of culture medium, cultivation temperature is controlled at 30～50 ℃, and incubation time is as the criterion with fermentation termination pH to 3～6;
2. bacterium powder or probiotic microcapsule preparation of granules:
After each strain fermentation finishes, get whole zymotic fluids and carry out centrifugal concentrating, get and concentrate back bacterium mud, add protective agent by 5～15% of bacterium mud mass ratio, carry out freeze drying or spray-drying after the emulsification evenly, make active bacterium powder of high concentration or probiotic microcapsule particle, wherein protective agent is a kind of or they mixed arbitrarily in powdered glucose, cane sugar powder, whey powder, skimmed milk power, lactose powder, serum powder, trehalose, sodium glutamate, glycerine, dusty yeast, the maltodextrin;
3. bacterium powder or probiotic microcapsule particle mix:
Two or more single strain powder or individual plant microcapsule granule are pressed living bacteria count and mass ratio is mixed, single strain powder or individual plant microcapsule granule addition are 0.05～50%, other adds mass ratio is that 0.01～10% effective component and mass ratio are 50～99.5% assistant carrier, and viable count is controlled at 10
6～10
9The cfu/ gram is prepared into the composite beverage solid portion.To add cryoprotector in freeze-drying as last, can make like this cell exempt the freezing initial stage because of forming the infringement that ice crystal causes.
The liquid part is the preparation method comprise:
(1) batching
A, title sample: take by weighing the fixedly component of liquid part, and two or more composition in the on-fixed component;
B, dissolving: the fixedly component that will take by weighing, and two or more composition in the on-fixed component adds appropriate amount of purified water respectively and carries out abundant dissolution process;
C, mixing: the single component that will dissolve respectively mixes by the priority program, stirs while adding during mixing, till fully mixing.
(2) sterilization
Carry out sterilization treatment with membrane filtering method.
In sum, the present invention has adopted plurality of proposals to solve the problem that the probio that has now in the probiotic beverage is difficult to long preservation.At first, composite beverage is divided into solid portion and liquid part, and uses the mode of solid to preserve probio, created good condition for the dormancy of probio; Secondly, on this basis, the excellent species of selecting typical bacterial classification has improved the survival rate of probio self as the probio in the composite beverage; At last, select suitable assistant carrier, the survival period of probio has been prolonged more than one times.In addition, the present invention also utilizes some bacterial classification Mixed culture mode to turn out the higher probio of survival rate, for example, and with Bacillus acidi lactici and streptococcus thermophilus ratio Mixed culture with 1: 1.
The specific embodiment
Understand concrete composition of the present invention for ease of those skilled in the art, the described composite beverage of present embodiment can be made up of the component of following mass ratio:
The liquid part (W/V, %): 1.0～25
Solid portion (W/W, %): the probiotics bacterial powder: 0.1～5 (containing protective agent)
Effective component: 0.01～10
Assistant carrier: 50～99.5
Wherein the collagen in the composite parts of liquid part is 1%～20% (W/V).
Wherein solid portion is prepared by following method:
1. strain fermentation:
Adopt following bacterial strain: lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, streptococcus thermophilus, saccharomyces cerevisiae, through single strain fermentation or mixing fermentation culture; The general technical process of slant strains that adopt to shake-flask seed to ferment tank; The media components that adopts generally includes carbon source, vitamin, amino acid and mineral matter and other components such as nitrogenous sources such as peptone, yeast extract, beef extract, sucrose, glucose; The fermentation liquid amount is generally the 70%-80% of volume, as two kinds of bacterium or the two or more bottle bacterial classification that shakes insert in the fermentation tank respectively when adopting Mixed culture, carry out fermented and cultured, obtain zymocyte liquid, fermentation is controlled at 4～8 (acid-base modifier is 1M NaOH or 1M HCl solution) with the initial pH of culture medium, cultivation temperature is controlled at 30～50 ℃, and incubation time is as the criterion with fermentation termination pH to 3～6;
2. bacterium powder or probiotic microcapsule preparation of granules:
After each strain fermentation finishes, get whole zymotic fluids and carry out centrifugal concentrating, get and concentrate back bacterium mud, add protective agent by 5～60% of bacterium mud mass ratio, carry out freeze drying or spray-drying after the emulsification evenly, make active bacterium powder of high concentration or probiotic microcapsule particle, wherein protective agent is a kind of or they mixed arbitrarily in powdered glucose, cane sugar powder, whey powder, skimmed milk power, lactose powder, serum powder, trehalose, sodium glutamate, glycerine, dusty yeast, the maltodextrin;
3. bacterium powder or probiotic microcapsule particle mix:
Two or more single strain powder or individual plant microcapsule granule are pressed living bacteria count and mass ratio is mixed, single strain powder or individual plant microcapsule granule addition are 0.05～50%, other adds mass ratio is that 0.01～10% effective component and mass ratio are 50～99.5% assistant carrier, and viable count is controlled at 10
6～10
9The cfu/ gram, preparation cost invention composite beverage solid portion.
For the preparation of finishing composite beverage solid portion of the present invention, and obtain the solid portion of composite beverage, can finish the preparation of this beverage by following making main points.
Make main points: take by weighing liquid fixedly component partly by formula rate (specifically seeing embodiment), and two or more composition in the on-fixed component; With the fixedly component that takes by weighing, and two or more composition in the on-fixed component adds appropriate amount of purified water respectively and carries out abundant dissolution process; The single component of dissolving is respectively mixed by the priority program, stir while adding during mixing, till fully mixing.Homogeneous transparent liquid with making carries out sterilization treatment with membrane filtering method, and can seals in the clean beverage bottle through sterilizing.Solid portion is after evenly mixing, and quantitative filling seals in the cavity of special plastic bottle cap.Bottle cap and beverage bottle are screwed in together, are the composite beverage (probio collagen oral liquid) that contains probio and collagen of the present invention.
Some concrete prescriptions that following embodiment is a composite beverage of the present invention, the technical staff can fill a prescription according to these, and obtains composite beverage according to the solid portion preparation method and the above-mentioned making main points of composite beverage of the present invention.
Embodiment
Example one: (dietary fiber)
Solid for mulation
Probio: bifidobacterium infantis 0.2%, Lactobacillus rhamnosus 0.1%, protective agent (adding) by bacterium mud mass ratio 20%
Functional component: do not add (can selectivity add)
Assistant carrier: dietary fiber 70%
Liquid formulations
Fixing component: collagen 15%,
The on-fixed component: extract of soybean 0.05%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.5 * 10 in the solid
8Individual/gram, just the shelf-life can reach 90 days at normal temperatures.
Example two: (glucose)
Solid for mulation
Probio: lactobacillus bulgaricus 0.2%, bifidobacterium bifidum 0.3%, protective agent (adding) by bacterium mud mass ratio 30%
Functional component: vitamin C 0.5%
Assistant carrier: glucose 50%
Liquid formulations
Fixing component: collagen 6%
The on-fixed component: kudzu root extract 0.1%, citric acid 1%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 2.0 * 10 in the solid
6Individual/gram, just the shelf-life can reach 90 days at normal temperatures.
Example three: (lactose)
Solid for mulation
Probio: bifidobacterium adolescentis 0.005%, lactobacillus reuteri 0.005%, protective agent (adding) by bacterium mud mass ratio 10%
Functional component: vitamin B6 2%
Assistant carrier: lactose 99%
Liquid formulations
Fixing component: collagen 10%
The on-fixed component: kudzu root extract 0.20%, corn syrup 8%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.8 * 10 in the solid
7Individual/gram, just the shelf-life can reach 90 days at normal temperatures.
Example four: (skimmed milk power)
Solid for mulation
Probio: bifidobacterium longum 0.2%, lactobacillus acidophilus 0.05%, protective agent (adding) by bacterium mud mass ratio 40%
Functional component: vitamin E 0.030%, Cobastab
10.2%
Assistant carrier: skimmed milk power 75%
Liquid formulations
Fixing component: collagen 4%
The on-fixed component: HFCS 10%, citric acid 0.1%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 2.1 * 10 in the solid
8Individual/gram, just the shelf-life can reach 90 days at normal temperatures.
Example five: (maltodextrin)
Solid for mulation
Probio: bifidobacterium breve 0.02%, brewer's yeast 0.02%, protective agent (adding) by bacterium mud mass ratio 50%
Functional component: vitamin A 0.018%, Cobastab
20.5%
Assistant carrier: maltodextrin 96%
Liquid formulations
Fixing component: collagen 8%
The on-fixed component: chondroitin 0.05%, HFCS 2%, fructose 6%, malic acid 0.5%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
9Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.3 * 10 in the solid
8Individual/gram, just the shelf-life can reach 90 days at normal temperatures.
Example six: (cane sugar powder)
Solid for mulation
Probio: bifidobacterium lactis 0.2%, streptococcus thermophilus 0.3%, protective agent (adding) by bacterium mud mass ratio 60%
Functional component: vitamin B6 0.01%, vitamin D
30.018%, Cobastab
120.6%
Assistant carrier: cane sugar powder 50%
Liquid formulations
Fixing component: collagen 12%
The on-fixed component: mountain slag inspissated juice 6%, malic acid 0.5%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.3 * 10 in the solid
8Individual/gram, just the shelf-life can reach 90 days at normal temperatures.
Example seven: (converted starch powder)
Solid for mulation
Probio: bifidobacterium lactis 0.002% (microcapsule bacterial powder), brewer's yeast 0.003%, protective agent (adding) by bacterium mud mass ratio 20%
Functional component: Cobastab
60.01%, Cobastab
121%
Assistant carrier: cane sugar powder 99.5%,
Liquid formulations
Fixing component: collagen 18%
The on-fixed component: Hami melon inspissated juice 6%, malic acid 0.5%, essence is an amount of.
Through check, solid portion contains total amount of probiotics and reaches 2.5 * 10 in the finished product
8Individual/gram, through 90 days preservation of room temperature, liquid does not partly obviously change in the product, and the probiotics viable bacteria sum reaches 1.6 * 10 in the solid
8Individual/gram, just the shelf-life can reach 90 days at normal temperatures.
Should be appreciated that those skilled in the art can make various changes or modifications the present invention after having read instruction foregoing of the present invention, these equivalent form of values fall within the application's appended claims equally and limit.
Claims (10)
1. composite beverage that contains probio and collagen, comprise solid portion and liquid part, described solid portion comprises probio at least, described liquid partly comprises fixedly component and on-fixed component, described fixedly component is a collagen, it is characterized in that described solid portion also comprises the assistant carrier that is used to prolong the probio survival period.
2. a kind of composite beverage that contains probio and collagen according to claim 1, it is characterized in that described assistant carrier is the mixture of a kind of, two kinds or multiple composition in powdered glucose, maltodextrin, cane sugar powder, lactose powder, dietary fiber, converted starch, resistant starch, corn flour, rice meal, the wheat flour etc., it is 50～99.5% the assistant carrier that is used to prolong the probio survival period that described solid portion comprises mass ratio (W/W).
3. a kind of composite beverage that contains probio and collagen according to claim 1 is characterized in that described solid portion comprises that also mass ratio (W/W) is 0.01～10% effective component, and described effective component is vitamin A, B
1, B
2, B
6, B
12, C, D
3, among the E one or more.
4. according to claim 1 or 2 or 3 described a kind of composite beverages that contain probio and collagen, it is characterized in that probio is two or more any compatibility in Bacillus acidi lactici, saccharomycete, Bifidobacterium, the streptococcus thermophilus.
5. a kind of composite beverage that contains probio and collagen according to claim 4, it is characterized in that Bacillus acidi lactici is lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, described Bifidobacterium is bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis.
6. according to claim 1 or 2 or 3 described a kind of composite beverages that contain probio and collagen, the viable count that it is characterized in that probio is 10
6～10
9The cfu/ gram.
7. according to claim 1 or 3 described a kind of composite beverages that contain probio and collagen, the gross mass volume ratio (W/V) that it is characterized in that described liquid part is 1.0～25%, and described on-fixed component is two or more any compatibility in chondroitin, extract of soybean, kudzu root extract, condensed water fruit juice, fructose, HFCS, corn steep liquor, citric acid, malic acid, the essence.
8. a method for preparing the described composite beverage of claim 1 comprises solid portion preparation method and liquid part preparation method, and described solid portion preparation method comprises:
1. strain fermentation
Adopt following probiotics strain: lactobacillus acidophilus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus bulgaricus, bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, streptococcus thermophilus, saccharomyces cerevisiae, through single strain fermentation or mixing fermentation culture, obtain zymocyte liquid, fermentation is controlled at 4～8 with the initial pH of culture medium, cultivation temperature is controlled at 30～50 ℃, and incubation time is as the criterion with fermentation termination pH 3～6;
2. bacterium powder or probiotic microcapsule preparation of granules
After each strain fermentation finishes, get whole zymotic fluids and carry out centrifugal concentrating, get and concentrate back bacterium mud, add protective agent, carry out freeze drying or spray-drying after the emulsification evenly, make active bacterium powder of high concentration or probiotic microcapsule particle by bacterium mud mass ratio 5～15%;
3. bacterium powder or probiotic microcapsule particle mix
Two or more single strain powder or probiotic microcapsule particle are pressed living bacteria count and mass ratio is mixed, single strain powder or individual plant microcapsule granule addition are 0.05～50%, other adds mass ratio is that 0.01～10% effective component and mass ratio are 50～99.5% assistant carrier, and viable count is controlled at 10
6～10
9The cfu/ gram is prepared into the composite beverage solid portion.
9. preparation method according to claim 8 is characterized in that described protective agent is a kind of or they are mixed arbitrarily in powdered glucose, cane sugar powder, whey powder, skimmed milk power, lactose powder, serum powder, trehalose, sodium glutamate, glycerine, dusty yeast, the maltodextrin.
10. the preparation method of described composite beverage according to Claim 8, described liquid part preparation method comprises:
(1) batching
A, title sample: take by weighing the fixedly component of liquid part, and two or more composition in the on-fixed component;
B, dissolving: the fixedly component that will take by weighing, and two or more composition in the on-fixed component adds appropriate amount of purified water respectively and carries out abundant dissolution process;
C, mixing: the single component that will dissolve respectively mixes by the priority program, stirs while adding during mixing, till fully mixing;
(2) sterilization
Carry out sterilization treatment with membrane filtering method.
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