CN112239726A

CN112239726A - Tool and method for quickly obtaining living probiotics with metabolism function and color change

Info

Publication number: CN112239726A
Application number: CN201910649250.0A
Authority: CN
Inventors: 杨继辉; 马仲俊
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-07-18
Filing date: 2019-07-18
Publication date: 2021-01-19

Abstract

The invention relates to a tool and a method for quickly obtaining living probiotics with metabolism function and discoloration. By the method, the living probiotics can be quickly and safely obtained in the required time, the defect that the living probiotics die quickly after being separated from a cold chain is overcome, and the risks of the living probiotics in the processes of stability, safety, short shelf life and transportation and storage are solved.

Description

Tool and method for quickly obtaining living probiotics with metabolism function and color change

Technical Field

The invention relates to a tool and a method for quickly obtaining living probiotics with metabolism function and discoloration, which are mainly used for preparing living probiotics such as medicines, foods, health products, animal feeds and the like.

Technical Field

According to the observation of the probiotic scale and the circulation field, the market scale of the probiotic products in China in 2010 is about 222 hundred million yuan, and the market scale of the probiotic products in the year 2017 is increased to 553 hundred million yuan, and the CAGR is equal to about 17%. However, the large market is limited to manufacturers who do living probiotics, and since the living probiotics are separated from the cold chain, do not supplement energy and are rapidly withered, the transportation, storage, safety, shelf life and stability cannot be guaranteed, and no manufacturers are willing to do the living probiotics. However, it is known from the relationship between probiotics and humans that only probiotics are colonized in the digestive tract of the human body in the form of living bodies to exert a therapeutic effect on diseases.

The strains written by commercial dry powder probiotic manufacturers can resist gastric acid and bile salt, but the strains are experiments under the precondition that the probiotic living form exists, but the commodities sold to consumers are in the form of dry powder, the probiotics in the form of dry powder belongs to deep dehydration dormant strains and does not have the capability of resisting gastric acid and bile salt, and although many manufacturers break through gastric acid and bile salt through special embedding technology or in the form of capsules. However, the probiotics take 5 hours to absorb water for survival, and the probiotics basically reach the large intestine to be discharged from the outside of the body after 5 hours from the time of oral administration of the probiotics, so that the dry powder probiotics can only solve diseases closely related to the large intestine, but the related diseases such as immunity, metabolism, digestion and the like are closely related to the stomach and the small intestine, so that the live probiotics have irreplaceable advantages.

Although it is possible that the freeze-dried powder probiotics can be colonized in the digestive tract in a small amount, population dominance is difficult to form, and thus the micro-ecological environment in the digestive tract cannot be modified. Only when the survival of the permanent planting is realized at different positions of the alimentary canal, the formation of population dominance can have good treatment effect on diseases. This can make people healthier. The living probiotics which can be planted in the alimentary canal in a large quantity are used by common people.

Disclosure of Invention

Freezing a probiotic solution into a solid state at (-1 to-50 ℃), directly subliming the solution into a gaseous state without a liquid state by water under vacuum (1.3 to 13Pa) to form probiotic powder, and then wrapping the powder by maltodextrin, sugar, vitamin c, lactose, glycerol, phytochrome (with an acid-base indication function) and the like to form dry powder. (the freeze-dried powder technology is very mature and is not specifically explained here).

The dry powder is placed at the bottom of the bottle cap, the customized resin grid and the glutinous rice starch film (the glutinous rice starch film is about 0.5mm thick and larger than the closed ring-shaped clamping groove of the bottle cap, and the glutinous rice starch film has good toughness and can not be damaged) are pressed and clamped in the ring-shaped clamping groove, and at the moment, the resin grid and the glutinous rice starch film can be clamped on the closed ring-shaped clamping groove, and the nitrogen aluminum film is added for sealing.

When in use, the aluminum film is torn, and the resin bottle cap is screwed on the resin bottle body.

The bottle body is held by hand and shaken for about 1 minute, the lower part of the closed ring is provided with an annular sealing ring to avoid liquid seepage in the shaking process until the sticky rice starch film and the probiotics are uniformly and completely dissolved in the liquid culture medium for inoculation.

The temperature is required to be above 5 ℃ and below 40 ℃, and the time is generally recommended to be up to 72 hours after 24 hours or to be taken after the liquid in the bottle is observed to change color.

Mixing ratio of culture medium and freeze-dried powder probiotics: 48.5% of dietary fiber and water (dendrobium solution with the concentration of 15%), 15% of protein powder, 16% of oligosaccharide, 20% of freeze-dried powder probiotics and 0.5% of plant pigment (acid-base indicating function).

The bottle cap is screwed to form a closed cuboid, so that whether the bottle cap is screwed can be directly observed. A small amount of gas is generated in the process of fermentation and propagation of the probiotics, and the square bottle can deform slightly and can also play a role in buffering.

Only the bottom of the whole bottle body is transparent for observing whether the liquid in the bottle changes color. Finally determining whether the probiotics start to metabolize.

The method solves the technical problems of transportation and storage of living probiotics (normal-temperature transportation and storage in the presence of dry powder and no requirement on energy supply), safety (stable and difficult change of physiological characteristics of strains in the presence of dry powder), long shelf life (no metabolism and no energy supply in the presence of dry powder) and stability (no reproductive capacity of dormant strains in the presence of dry powder). The probiotic bacteria have dormant strains without metabolism function in a freeze-drying dehydration state, are very stable and convenient for transportation and storage, but do not belong to living probiotic bacteria, and the living probiotic bacteria are obtained from 5 hours to 72 hours after the technology is used. The technology inherits all the advantages of probiotics in the form of freeze-dried powder and can quickly obtain live probiotic strains for treating or intervening diseases. The bacterial strain has the advantages that (1) the bacterial strain can be planted in any position of the whole digestive tract in a highly efficient manner (because the living probiotics have strong capability of resisting gastric acid and bile salt) (2) the bacterial strain can be planted in the esophagus, the stomach, the small intestine and the large intestine accurately (5 hours are needed for the freeze-dried and dehydrated bacterial strain to absorb water and survive), the bacterial strain can only be planted in the large intestine and cannot treat or interfere other diseases of the digestive tract) (3) the accurate observation is carried out, the living probiotics can generate a large amount of biological enzymes, organic acids and polypeptide substances in a culture medium solution to regulate diseases of a body, wherein the organic acids can react with phytochrome in the culture medium solution, and whether the living probiotics exist can be directly observed through color change. (4) The working speed is high, the living probiotics can antagonize harmful bacteria and produce organic acid biological enzyme and other beneficial body products quickly after entering the body, thereby quickly regulating diseases. In conclusion, by such a method, live probiotics can be obtained quickly in the required time.

The prior art needs whole cold chain transportation and storage, and because the live bacterial strain possesses the function of metabolic death reproduction and needs continuous energy supply, the shelf life is short and uncontrollable, if factors such as power failure occur in cold chain storage, the live bacterial strain wakes up and is dead due to not having too much energy supply, and the risk is great.

In conclusion, several important factors affecting the microecology of the digestive tract are the type, the quantity and the living body, respectively, the type and the quantity can be solved in the dry powder bottle cap, and the living body needs the technical breakthrough applied at present.

Drawings

Fig. 1, fig. 3, fig. 4, fig. 5 and fig. 6 show the structure of the tool bottle cap for living probiotics.

Probiotic tool bottle and cross-sectional view as shown in figure 2

The method comprises the following specific implementation steps:

1. freezing a probiotic solution into a solid state at (-1 to-50 ℃), directly subliming the solution into a gaseous state without a liquid state by water under vacuum (1.3 to 13Pa) to form probiotic powder, and then wrapping the powder by maltodextrin, sugar, vitamin c, lactose, glycerol, phytochrome (with an acid-base indication function) and the like to form dry powder. (the freeze-dried powder technology is very mature and is not specifically explained here).

2. The dry powder is placed at the bottom of the bottle cap, the customized resin grid and the glutinous rice starch film (the glutinous rice starch film is about 0.5mm thick and larger than the closed ring-shaped clamping groove of the bottle cap, and the glutinous rice starch film has good toughness and can not be damaged) are pressed and clamped in the ring-shaped clamping groove, and at the moment, the resin grid and the glutinous rice starch film can be clamped on the closed ring-shaped clamping groove, and the nitrogen aluminum film is added for sealing.

3. When in use, the aluminum film is torn, and the resin bottle cap is screwed on the resin bottle body.

4. The bottle body is held by hand and shaken for about 1 minute, the lower part of the closed ring is provided with an annular sealing ring to avoid liquid seepage in the shaking process until the sticky rice starch film and the probiotics are uniformly and completely dissolved in the liquid culture medium for inoculation.

5. The temperature is required to be above 5 ℃ and below 40 ℃, and the time is generally recommended to be up to 72 hours after 24 hours or to be taken after the liquid in the bottle is observed to change color.

6. Mixing ratio of culture medium and freeze-dried powder probiotics: 48.5% of dietary fiber and water (dendrobium solution with the concentration of 15%), 15% of protein powder, 16% of oligosaccharide, 20% of freeze-dried powder probiotics and 0.5% of plant pigment (acid-base indicating function).

7. The bottle cap is screwed to form a closed cuboid, so that whether the bottle cap is screwed can be directly observed. A small amount of gas is generated in the process of fermentation and propagation of the probiotics, and the square bottle can deform slightly and can also play a role in buffering.

8. Only the bottom of the whole bottle body is transparent and is used for observing whether the liquid in the bottle changes color or not, and finally determining whether the probiotics start to metabolize or not.

Claims

1. The method for quickly obtaining the living probiotics with metabolic function and discoloration comprises the following steps:

(1) freezing a probiotic solution into a solid state at (-1 to-50 ℃), directly subliming the liquid state into a gaseous state to form probiotic powder by water under vacuum (1.3 to 13Pa), and then wrapping the probiotic powder by maltodextrin, sugar, vitamin c, lactose, glycerol, phytochrome (with an acid-base indication function) and the like to form dry powder (the freeze-dried powder technology is mature and is not specifically explained);

(2) placing the dry powder at the bottom of a bottle cap, pressing and clamping a customized resin grid and a glutinous rice starch film (the glutinous rice starch film is about 0.5mm in thickness and larger than a closed annular clamping groove of the bottle cap, and the glutinous rice starch film has good toughness and cannot be damaged) in the annular clamping groove, clamping the resin grid and the glutinous rice starch film on the closed annular clamping groove, and adding a nitrogen aluminum film for sealing;

(3) when in use, the aluminum film is torn, and the resin bottle cap is tightly screwed on the resin bottle body;

(4) the bottle body is held by hand and shaken for about 1 minute, the lower part of the closed ring is provided with an annular sealing ring to avoid liquid seepage in the shaking process until the sticky rice starch film and the probiotics are uniformly and completely dissolved in the liquid culture medium for inoculation;

(5) the temperature is required to be above 5 ℃ and below 40 ℃, and the time generally suggests that the liquid can be taken within 24 hours or less than 72 hours at most or can be taken after the liquid in the bottle is observed to change color;

(6) and the mixing proportion of the culture medium and the freeze-dried powder probiotics is as follows: 48.5% of dietary fiber and water (dendrobium solution with the concentration of 15%), 15% of protein powder, 16% of oligosaccharide, 20% of freeze-dried powder probiotics and 0.5% of plant pigment (acid-base indicating function);

(7) the bottle cap is screwed tightly to form a closed cuboid, so that whether the bottle cap is screwed tightly can be directly observed, a small amount of gas is generated in the fermentation and propagation processes of probiotics, and the square bottle can deform slightly and can also play a buffering role;

(8) and only the bottom of the whole bottle body is transparent and is used for observing whether the liquid in the bottle changes color or not, and finally determining whether the probiotics start to metabolize or not.