CN101659952B - 锁核酸和小沟结合物共修饰核酸片段 - Google Patents

锁核酸和小沟结合物共修饰核酸片段 Download PDF

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Publication number
CN101659952B
CN101659952B CN2009100548053A CN200910054805A CN101659952B CN 101659952 B CN101659952 B CN 101659952B CN 2009100548053 A CN2009100548053 A CN 2009100548053A CN 200910054805 A CN200910054805 A CN 200910054805A CN 101659952 B CN101659952 B CN 101659952B
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nucleic acid
oligonucleotide
lna
mgb
probe
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Chinese (zh)
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CN101659952A (zh
Inventor
邵俊斌
沈步超
倪卫琴
赵红喜
朱勤玮
张彬彬
马丽丽
舒峰
朱旭平
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Shanghai ZJ Bio Tech Co Ltd
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SHANGHAI ZHIJIANG BIOTECHNOLOGY CO Ltd
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Priority to CN2009100548053A priority Critical patent/CN101659952B/zh
Priority to PCT/CN2009/072848 priority patent/WO2011006306A1/fr
Publication of CN101659952A publication Critical patent/CN101659952A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2009100548053A 2009-07-14 2009-07-14 锁核酸和小沟结合物共修饰核酸片段 Active CN101659952B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2009100548053A CN101659952B (zh) 2009-07-14 2009-07-14 锁核酸和小沟结合物共修饰核酸片段
PCT/CN2009/072848 WO2011006306A1 (fr) 2009-07-14 2009-07-21 Fragments d'acide nucléique co-modifiés par à la fois un acide nucléique bloqué et un ligand du petit sillon

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Application Number Priority Date Filing Date Title
CN2009100548053A CN101659952B (zh) 2009-07-14 2009-07-14 锁核酸和小沟结合物共修饰核酸片段

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CN101659952A CN101659952A (zh) 2010-03-03
CN101659952B true CN101659952B (zh) 2012-07-18

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CN (1) CN101659952B (fr)
WO (1) WO2011006306A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102433376B (zh) * 2011-10-19 2013-07-31 上海千友生物科技有限公司 基于荧光淬灭的基因变异检测方法及探针
WO2013075079A1 (fr) * 2011-11-17 2013-05-23 Rheonix, Inc. Système et procédés pour analyse moléculaire sélective
CN104293937B (zh) * 2014-09-28 2016-12-07 广东省妇幼保健院 一组基于锁核酸增敏的液相芯片检测地中海贫血基因点突变的探针、检测试剂盒及检测方法
CN104531852A (zh) * 2014-12-12 2015-04-22 广东省妇幼保健院 一种锁核酸增敏的检测mthfr基因c677t突变的探针及引物、试剂盒和检测方法
CN105671204B (zh) * 2016-03-15 2019-07-02 山东省动物疫病预防与控制中心 Ndv/h9-aiv/ibv三重荧光pcr检测试剂
CN109251977A (zh) * 2018-02-14 2019-01-22 重庆京因生物科技有限责任公司 基于poct模式的slco1b1基因型快速检测试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1833034A (zh) * 2003-06-20 2006-09-13 埃克斯魁恩公司 用于分析核酸混合物的探针、文库和试剂盒及其构建方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7205105B2 (en) * 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
US7348146B2 (en) * 2003-10-02 2008-03-25 Epoch Biosciences, Inc. Single nucleotide polymorphism analysis of highly polymorphic target sequences
SI1687609T1 (sl) * 2003-10-28 2015-03-31 Epoch Biosciences, Inc. Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem
GB2413796B (en) * 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
WO2007115590A1 (fr) * 2006-04-11 2007-10-18 Technische Universität Wien Détection et quantification d'une pollution fécale dans un échantillon environnemental

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1833034A (zh) * 2003-06-20 2006-09-13 埃克斯魁恩公司 用于分析核酸混合物的探针、文库和试剂盒及其构建方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Letertre C.et al..Evaluation of the performance of LNA and MGB probes in 5"-nuclease PCR assays.《Molecular and cellular probes》.2003,第17卷(第6期),307-311. *
郑夔等.一种无RNA纯化步骤TaqMan RT-PCR方法用于快速检测登革病毒.《华南预防医学》.2008,第34卷(第3期),6-9,14. *

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WO2011006306A1 (fr) 2011-01-20
CN101659952A (zh) 2010-03-03

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Address after: 201201 Shanghai City, Pudong New Area Zhangjiang hi tech Industrial District Ruiqinglu No. 528 building 20 B No. 1 building 21 layer, a layer of 1

Patentee after: Shanghai ZJ Bio-Tech Co., Ltd.

Address before: 201203 Shanghai Zhangjiang High Tech Park of Pudong New Area Cailun Road No. 720 Building 1 room 317

Patentee before: Shanghai Zhijiang Biotechnology Co., Ltd.