CN101642488A - Scutellaria composite immune reinforcing agent, preparation method thereof and application thereof - Google Patents
Scutellaria composite immune reinforcing agent, preparation method thereof and application thereof Download PDFInfo
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- CN101642488A CN101642488A CN200910101574A CN200910101574A CN101642488A CN 101642488 A CN101642488 A CN 101642488A CN 200910101574 A CN200910101574 A CN 200910101574A CN 200910101574 A CN200910101574 A CN 200910101574A CN 101642488 A CN101642488 A CN 101642488A
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Abstract
The technology belongs to the technical field of aquiculture disease prevention, in particular to the preparation method of the immune reinforcing agent for cultivating crustacean, and the applicationmethod thereof. The aquiculture composite immune reinforcing agent comprises 2-4 parts of aquiculture extractive or baicalin and 2.5-5 parts of levamisole. The composite immune reinforcing agent caneffectively enhance non-specific immunity for cultivating blue crab, improve the activity of the blood serum phenol oxidase, the superoxide dismutase and the alkalinephosphatase of the blue crab, andimproves the disease resistance of the blue crab to common pathogens such as white spot syndrome virus, vibrio, hematodinium, etc. The composite immune reinforcing agent can be applied to the cultivation of the blue crab by being injected or added into blue crab fresh fish bait and granulated feed for preventing and controlling the disease at the disease peak and reducing the disease rate and themortality rate of the blue crab. The immune reinforcing agent is added into the feed for preventing and controlling the disease of the penaeus vannamei boone and exopalaemon carinicauda.
Description
Technical field
Present technique belongs to technical field of aquiculture disease prevention, relates in particular to a kind of methods for making and using same of culturing the shell-fish immunostimulant.
Technical background
The sea water economic crab is the important component part of China's sea-farming, the sea crabs class principal item that China cultures is Scylla serrata (Forskal). (Scylla serrata Forskal) and swimming crab (Portunus sp), belong to Crustachia (Crustacea), Decapoda (Decapoda), Portumidae (Portunidae), be distributed widely in marine sites such as Deposits in Eastern Coastal China each province and Japan, India, Southeast Asia, Australia, South Africa, being the important marine economy Eriocheir sinensis class of China, also is the important export kind of earning foreign exchange.In recent years, China's sea crabs class aquaculture develops rapidly, and China Scylla serrata (Forskal). in 2006 and swimming crab cultured area reach 76,277 ten thousand hectares, account for more than 95% of sea crabs cultured area, and both total outputs reach about 220,000 tons.Wherein Scylla serrata (Forskal). output reaches 10.85 ten thousand tons, accounts for 88.14% of the total cultured output in the world, is the first in the world blue crab cultivation big country.In addition, Litopenaeus vannamei of China (Litopenaeus vannameie) (being commonly called as Penaeus vannamei) and dusky white prawn (Exopalamon carincauda Holthuis) (claiming little white shrimp again) are the important economic shrimps in culture of China, wherein the cultured output of Penaeus vannamei is that dusky white prawn occupied an important position in coastal breed first of whole economic shell-fish were cultured.
Along with the large-scale development that sea crabs is cultured, disease becomes the major obstacle of culturing development day by day.In recent years, comparatively serious disease has successively taken place in mud crab and swimming crab, and wherein culturing the mud crab average attack rate is about 13.77%, average mortality 5.43%, the economic loss that causes every year surplus in the of 200,000,000 yuan.Disease has caused great economic loss also for the breed swimming crab.In the disease control at present, mainly adopting medicine is main control device, and expense is higher, and a large amount of uses of antibacterials also cause a series of problems such as cultivated crabs drug residue, Resistant strain generation, have greatly influenced the quality of cultivated crabs shrimp.
Summary of the invention
In order to solve the technical problem that exists in the above-mentioned aquaculture, an object of the present invention is to provide a kind of scutellaria composite immune reinforcing agent, it not only has good insecticidal action, can also significantly improve the opposing level of mud crab to vibrio infection, strengthens the immunity of crustacean.
Second purpose of the present invention provides the preparation method of above-mentioned scutellaria composite immune reinforcing agent.
The 3rd purpose of the present invention provides the novel form that above-mentioned scutellaria composite immune reinforcing agent is made.
In order to realize first above-mentioned purpose, the present invention has adopted following technical scheme:
Scutellaria composite immune reinforcing agent, this composite immune reinforcing agent comprise Radix Scutellariae extract or baicalin and levamisole, and the parts by weight of Radix Scutellariae extract or baicalin and levamisole are as follows:
2～4 parts of Radix Scutellariae extract or baicalins
2.5～5 parts of levamisoles.
As most preferably, the parts by weight of Radix Scutellariae extract or baicalin and levamisole are as follows:
2.5 parts of Radix Scutellariae extract or baicalins
3 parts of levamisoles.
Above-mentioned baicalin (Baicalin) another name baicalin, available from domestic manufacturer, the content of baicalin of product is not less than 95%, and all the other features meet the requirement of Chinese Pharmacopoeia 2005 editions (I portion).
Above-mentioned Radix Scutellariae extract can adopt following method: 1. get Radix Scutellariae (parched), and after fully pulverizing, the adding distil water soaked overnight; 2. decoct boiling 30min, moisturizing decocts boiling 30min again, with gauze elimination medicinal residues, gets supernatant; 3. 2. moisturizing decocts and the elimination medicinal residues with step, decocts two twice again; 4. with the further decocting and concentrating of medicinal liquid that cooks for 3 times, remove by filter granule, get the clarification filter liquor; 5. add NaOH, add 3 times of volume 95% ethanol again, thermal agitation; 6. after leaving standstill, the centrifugal 15min of 8000r/min gets the brown precipitation; 7. add entry dissolution precipitation again, the centrifugal insoluble matter of removing adds NaOH and 3 times of volume 95% ethanol again, with 5. centrifugation of step, repeats twice, the dry Radix Scutellariae extract goods that get.Certainly, the preparation method of Radix Scutellariae extract should be not limited to and such scheme, and for the method for existing Chinese medicine extraction, the present invention all can be suitable for.
In order to realize second above-mentioned purpose, the preparation method of scutellaria composite immune reinforcing agent of the present invention is got Radix Scutellariae extract or baicalin and levamisole and is fully mixed with blender, and shady and cool lucifuge place preserves.
In order to realize the 3rd above-mentioned purpose, the present invention has adopted following technical scheme:
One, the Radix Scutellariae complex immunity strengthens injection, and this injection adopts the described scutellaria composite immune reinforcing agent of above-mentioned any one technical scheme, adds dissolved in distilled water, adds Na again
2CO
3The solution hydrotropy stirs or vibration, and with the normal saline dilution, it is 0.5%～4.0% that described scutellaria composite immune reinforcing agent accounts for total weight percent concentration then, and preferred concentration is 1%～4.0%.
Two, the Radix Scutellariae complex immunity strengthens feedstuff, and this feedstuff comprises the described scutellaria composite immune reinforcing agent dough-making powder of above-mentioned any one technical scheme, and scutellaria composite immune reinforcing agent adopts Na
2CO
3Mix with flour or flour and forage fiss behind the solution hydrotropy, it is 4.5%～10.0% that described scutellaria composite immune reinforcing agent accounts for total weight percent; Perhaps, make pellet by the consumption of the described scutellaria composite immune reinforcing agent of feedstuff adding 0.2kg～1.0kg per ton.
Scutellaria composite immune reinforcing agent of the present invention is used for preparing prevention or the medicine of shell-fish infectious disease or the application of feedstuff are cultured in treatment; Perhaps, scutellaria composite immune reinforcing agent of the present invention is used for preparing the medicine of raising breed shell-fish immunity or the application of feedstuff.As preferably, described infectious disease is the pathogenicity infectious disease.Preferred pathogenicity disease is the pathogenicity disease that the infection of white spot syndrome virus infection, the whirlpool whipworm infection of blood ovum and other ciliate, vibrio causes.Preferred breed shell-fish is mud crab, swimming crab, Penaeus vannamei or dusky white prawn.
The inventor finds that Radix Scutellariae extract has immunological enhancement preferably to mud crab on the extensive screening basis of Chinese herbal medicine immunostimulant, can strengthen the main immune indexes of mud crab serum comprehensively, improves the kill ability of mud crab serum to vibrio.Measure in the mud crab body and show: Radix Scutellariae extract has good potentiation to immune relevant enzymes such as mud crab serum phenol oxidase, superoxide dismutase, alkali phosphatases, can strengthen the anti-vibrio level of mud crab serum, strengthen mud crab hemocyte phagocytic rate, and can significantly improve the opposing level of mud crab vibrio infection.Industrial experimentation shows that Radix Scutellariae extract can improve the mud crab premunition, reduce the mud crab mortality rate of the autumn and the phase mud crab onset peak of surviving the winter, all can reach effect preferably by injecting and throwing something and feeding, wherein inject be suitable for surviving the winter stage mud crab enhancing immunity and disease prevention, and add the disease control that relatively is suitable for onset peak in the breeding process in the feedstuff to.Consider the fresh foods such as fish and shrimp that the geographic sea water crayfish culture of present major part adopts natural waters to catch more, so the method for adding immunostimulant in fresh food and the pellet has been set up in inventor's design respectively.
The inventor finds that in the research to Radix Scutellariae extract the Main Ingredients and Appearance of Radix Scutellariae extract is a baicalin, and laboratory and productivity application test show: commercially available baicalin has same immunological enhancement to mud crab.The main active ingredient of Radix Scutellariae extract has been illustrated in research, and makes the large-scale production of immunostimulant and application become possibility.In addition, shrimp white spot syndrome virus (WSSV) and blood ovum whirlpool whipworm are the main pathogen of mud crab, swimming crab disease, and still not a kind of important insecticidal materials of levamisole strengthens the immunity of crustacean in addition.Inventor's studies show that levamisole and baicalin are promoting to also have certain synergism aspect the phenoloxidase activity.
The inventor has determined that the main active ingredient of Radix Scutellariae extract is a baicalin, and has determined baicalin and the levamisole immune activation dosage to mud crab, has determined the best matched proportion density of Radix Scutellariae and levamisole.And set up three kinds of immunostimulants such as injection, spice method and pellet processing method according to the blue crab cultivation production status and used approach, be applicable to the different breeding mud crab in period respectively.Test shows that also this immunostimulant also can add in the feedstuff, is applied to the disease prevention that is used for Penaeus vannamei and dusky white prawn and the control of Penaeus vannamei and dusky white prawn.
Scutellaria composite immune reinforcing agent of the present invention can be used for the disease control of the following aspects:
1) disease prevention and the control of breed pool mud crab: cause that mud crab morbidity main causes of death have the secondary infection of white spot syndrome virus (WSSV) infection, the whirlpool whipworm infection of blood ovum and other ciliate, vibrio etc., disease season occurred frequently is the annual 9-10 month, water temperature is fit to the breeding of water body pathogen during this, add rainwater, temperature Change etc. causes the bigger stress of mud crab round the clock, self-resistance is relatively poor.Scutellaria composite immune reinforcing agent of the present invention to this in period the mud crab disease good preventing and control action are arranged.Owing to culture the fresh food of throwing something and feeding on the mud crab more during this, can adopt composite immune reinforcing agent and fresh food to mix the back feeding method thoroughly.Before onset peak, can throw something and feed and add the granular bait for fish of composite immune reinforcing agent, threw something and fed continuously 3-5 days, good protection effect is arranged.
2) the survive the winter prevention and the control of phase mud crab disease: the phase of surviving the winter is another peak of mud crab death, and this mud crab feed in period is less, and employing intramuscular injection composite immune reinforcing agent is an admissible method mud crab is put in a suitable place to breed before.Also can before mud crab advances wintering pond, composite immune reinforcing agent be added feedstuff and throw something and feed continuously mud crab 3-4 days, with the survive the winter premunition of mud crab of raising.
3) prevention and control of Penaeus vannamei and dusky white prawn disease: the principal disease of Penaeus vannamei and dusky white prawn disease is white spot syndrome virus (WSSV) and all kinds of vibrios etc., and water quality deterioration also can become the inducement that disease takes place.Studies show that: composite immune reinforcing agent has disease prevention preferably and control effect to Penaeus vannamei and dusky white prawn, and the pellet that adopts composite immune reinforcing agent the to add grow shrimp of throwing something and feeding can obtain good control effect.
The specific embodiment
Embodiment 1
One, Radix Scutellariae extract extracts from the Chinese crude drug Radix Scutellariae (parched), and concrete preparation method is as follows:
1. buy Radix Scutellariae (parched) from the shop of Chinese medicines, get Radix Scutellariae (parched) 100g, after fully pulverizing, 4 ℃ of soaked overnight of adding distil water 500mL;
2. the Radix Scutellariae soak is used and decocted boiling 30min, moisturizing 250mL decocts boiling 30min again, uses gauze elimination medicinal residues, gets supernatant;
3. leach medicinal residues and add water 250ml,, decoct again twice with 2. decocting and the elimination medicinal residues;
4. the medicinal liquid that cooks for 3 times is merged, further decocting and concentrating removes by filter granule to 100mL, gets clear filtrate;
5. in the clarification filter liquor, add 0.5M NaOH 2mL, add 3 times of volume 95% ethanol again, thermal agitation;
6. after leaving standstill 4h, the centrifugal 15min of 8000r/min gets the brown precipitation;
7. add 50mL water dissolution precipitation again, the centrifugal insoluble matter of removing adds 0.5M NaOH 1mL and 3 times of volume 95% ethanol again, with 5. centrifugation, repeats twice, the dry Radix Scutellariae extract goods that get.
Embodiment 2
The preparation method of scutellaria composite immune reinforcing agent: get among the embodiment 1 2.5 parts of the Radix Scutellariae extract dry products of preparation, 3 parts of levamisoles fully mix with blender, and shady and cool lucifuge place preserves.
Levamisole is levamisole hydrochloride for animals (levamisole hydrochloride), and available from domestic manufacturer, its feature meets the requirement of Chinese veterinary drug allusion quotation 2005 editions (I portion).
Embodiment 3
In view of the Radix Scutellariae extract Main Ingredients and Appearance is a baicalin, so also available Radix Scutellariae substitutes Radix Scutellariae extract.The preparation method of scutellaria composite immune reinforcing agent: get 2.5 parts of baicalins, 3 parts of levamisoles fully mix with blender, and shady and cool lucifuge place preserves.
Baicalin (Baicalin) another name baicalin, available from domestic manufacturer, the content of baicalin of product is not less than 95%, and all the other features meet the requirement of Chinese Pharmacopoeia 2005 editions (I portion).
Embodiment 4
The preparation method of scutellaria composite immune reinforcing agent: get among the embodiment 1 4.0 parts of the Radix Scutellariae extract dry products of preparation, 2 parts of levamisoles fully mix with blender, and shady and cool lucifuge place preserves.
Embodiment 5
The preparation method of scutellaria composite immune reinforcing agent: get 4.0 parts of baicalins, 2 parts of levamisoles fully mix with blender, and shady and cool lucifuge place preserves.
Embodiment 6
The intramuscular injection of composite immune reinforcing agent:
Intramuscular injection to mud crab and other Eriocheir sinensis classes is carried out as follows:
1) gets the scutellaria composite immune reinforcing agent 12g that embodiment 2～7 prepares, use the 20mL dissolved in distilled water, add the Na of 1mL 0.1M
2CO
3Solution stirs or vibration is dissolved to basic, and moisturizing is to 100mL;
2) with the lytic immunity reinforcing agent with 0.2 μ m sterilising filter filtration sterilization, aseptic be stored in 4 ℃ standby;
3) use before, should be with immunopotentiator solution with 25 ℃ of water-bath preheatings, with the normal saline of 25 ℃ of water-bath preheatings immunopotentiator solution is diluted 5 times; Injected dose gets the average weight of Eriocheir sinensis by mud crab.Press 100g mud crab 0.2mL dosage injection mud crab step base portion, injection portion is all sterilized with alcohol swab before and after the injection;
4) after injection finishes mud crab support is observed 0.5-1h temporarily, can put back to after no abnormal and culture in the pond.
Embodiment 7
Throw something and feed after composite immune reinforcing agent and fresh food are mixed thoroughly: get embodiment 2～7 and prepare immunostimulant 50g, water 100mL dissolving adds 0.5mL 0.1M Na
2CO
3The solution hydrotropy, 100kg mixes with forage fiss, adds the abundant mixing of flour 0.5～1kg, the mud crab of throwing something and feeding.
Embodiment 8
Composite immune reinforcing agent method of preparing pellet feed: make pellet by the consumption that feedstuff interpolation 0.5kg embodiment 2～7 per ton prepares immunostimulant, can adopt the normal feedstuff of mud crab, Penaeus vannamei or dusky white prawn according to the object of throwing something and feeding respectively, be respectively applied in the disease prevention of mud crab, Penaeus vannamei, dusky white prawn.
Test example 1: the prevention and control of diseases test of the phase Scylla serrata (Forskal). that survives the winter
The present embodiment and result take from inventor's winter test data, and test method and result are summarized as follows:
1) compound scutellaria composite immune reinforcing agent group and matched group are established in test, inject compound Radix Scutellariae reinforcing agent group (embodiment 2) and normal saline respectively, and dosage is undertaken by summary of the invention 3 intramuscular injections.According to the body weight difference of Eriocheir sinensis, each group is at step and back side intersection injecting immune polysaccharide 0.15-0.3mL, and 30 mud crabs of every group of injection are put mud crab in a suitable place to breed in 300cm * 200cm * 150cm net cage after the injection; The duration of test water temperature is 10 ± 2 ℃, and salinity is 19.5 ‰.The mud crab liquid of haemolymph is extracted in 3 of injection back 8,16,24,32d samplings, measures the related immune index of mud crab serum.
2) result shows: behind the mud crab injection scutellaria composite immune reinforcing agent, serum alkaline phosphatase (ALP) and superoxide dismutase (SOD) vigor all are significantly higher than matched group, wherein do not see obvious decline behind the horizontal 32d of ALP, the SOD vigor can be kept 16d, serum oxidase (POD) and phenol oxidase (PO) vigor also significantly raise, wherein the PO comparison shows that according to having improved 176% immunostimulant has significant immunological enhancement.Statistics is used the survival rate of immunostimulant group and matched group, the results are shown in Table 1.By table as seen, the survival rate of immunostimulant group is 82% behind the breed 45d, and matched group is 61%, and immune group is significantly increased than matched group.
Table 1 immunostimulant is to the influence of Scylla serrata (Forskal). survival rate
The survive the winter prevention and control of diseases industrial experimentation of phase Scylla serrata (Forskal). of test example 2
The present embodiment and result take from inventor's industrial experimentation data of surviving the winter, test method and result are summarized as follows: test is carried out in the Serpentis island magnificent breed company of shaking that coils, the said firm cultures for surviving the winter on a large scale and uses pilot, adopt indoor cement pit aquaculture model, the duration of test water temperature is 15 ± 2 ℃, and salinity is 19.5 ‰.Test group injection scutellaria composite immune reinforcing agent (embodiment 2), contrast is not injected, and measures the related immune index behind the breed injection 15d, and statistics is cultured the survival rate that 30d respectively organizes mud crab.The results are shown in Table 2.
The application result of table 2 immunostimulant in Scylla serrata (Forskal). productivity wintering pond
By table as seen, injection scutellaria composite immune reinforcing agent group, each immune indexes of serum is significantly improved than matched group behind the 15d, wherein obvious with phenol oxidase in the serum (PO), superoxide dismutase (SOD) and alkali phosphatase (ALP), immunostimulant group mud crab survival rate 88% behind the injection 30d, the survival rate of matched group only is 59%.Show that immunostimulant can significantly improve the survival rate that mud crab survives the winter.
The effect of test example 3 scutellaria composite immune reinforcing agents in mud crab and dusky white prawn prevention and control of diseases
This result of implementation is taken from the inventor and is prepared immunostimulant interpolation pellet result.Test method and result are summarized as follows: get 2.5 parts of baicalins, 3 parts of composite immune reinforcing agents that mix of levamisole, be added in the feedstuff by feedstuff 0.5kg addition per ton, normal feedstuff adopts the Penaeus vannamei material formula, is prepared as pellet.The production use amount is the feedstuff 20kg that throws something and feeds before per 20 mu of big or small Eriocheir sinensis pool onset peaks, throws something and feeds morning, and 3-5d continuously throws something and feeds.The effect of investigation user.The results are shown in Table 3.
The application result of table 3 immunostimulant agent feedstuff during onset peak mud crab or dusky white prawn are cultured in the fall
Annotate: feed intake afternoon.
By table as seen, behind the scutellaria composite immune reinforcing agent feedstuff of throwing something and feeding, the mud crab of 7 breeding-grounds or dusky white prawn death have obvious minimizing, and the vigor of catching the listing mud crab also obviously strengthens.Because test tank early stage except that a mud crab monoculture pond, all based on fresh foods such as the fish and shrimp of throwing something and feeding, is to increase ingesting of breed variety, require raiser's feedstuff of throwing something and feeding in the morning, the fresh food of throwing something and feeding afternoon.The raiser of one of them failure, possible cause is: early stage is morbidity obviously, and mistake feeds intake in the afternoon again, and the breed variety immunity enhanced feed of can not in time taking food causes residual bait polluted-water on the contrary, has aggravated disease.
Can think disease prevention and control when the pellet of interpolation immunostimulant can be used for mud crab and dusky white prawn onset peak by this test.
Claims (10)
1. scutellaria composite immune reinforcing agent is characterized in that this composite immune reinforcing agent comprises Radix Scutellariae extract or baicalin and levamisole, and the parts by weight of Radix Scutellariae extract or baicalin and levamisole are as follows:
2～4 parts of Radix Scutellariae extract or baicalins
2.5～5 parts of levamisoles.
2. scutellaria composite immune reinforcing agent according to claim 1 is characterized in that the parts by weight of Radix Scutellariae extract or baicalin and levamisole are as follows:
2.5 parts of Radix Scutellariae extract or baicalins
3 parts of levamisoles.
3. scutellaria composite immune reinforcing agent according to claim 1 and 2 is characterized in that the preparation method of Radix Scutellariae extract is as follows:
1. get Radix Scutellariae (parched), after fully pulverizing, the adding distil water soaked overnight;
2. decoct boiling 30min, moisturizing decocts boiling 30min again, with gauze elimination medicinal residues, gets supernatant;
3. 2. moisturizing decocts and the elimination medicinal residues with step, decocts two twice again;
4. with the further decocting and concentrating of medicinal liquid that cooks for 3 times, remove by filter granule, get the clarification filter liquor;
5. add NaOH, add 3 times of volume 95% ethanol again, thermal agitation;
6. after leaving standstill, the centrifugal 15min of 8000r/min gets the brown precipitation;
7. add entry dissolution precipitation again, the centrifugal insoluble matter of removing adds NaOH and 3 times of volume 95% ethanol again, with 5. centrifugation of step, repeats twice, the dry Radix Scutellariae extract goods that get.
4. the preparation method of scutellaria composite immune reinforcing agent according to claim 1 and 2 is characterized in that this method is as follows: get Radix Scutellariae extract or baicalin and levamisole and fully mix with blender, the preservation of shady and cool lucifuge place.
5. the Radix Scutellariae complex immunity strengthens injection, it is characterized in that: this injection adopts claim 1 or 2 described scutellaria composite immune reinforcing agents, adds dissolved in distilled water, adds Na again
2CO
3The solution hydrotropy stirs or vibration, and with the normal saline dilution, it is 0.5%～4.0% that described scutellaria composite immune reinforcing agent accounts for total weight percent concentration then.
6. the Radix Scutellariae complex immunity strengthens feedstuff, it is characterized in that: this feedstuff comprises claim 1 or 2 described scutellaria composite immune reinforcing agent dough-making powder or flour, forage fiss, and scutellaria composite immune reinforcing agent adopts Na
2CO
3Mix with flour or flour, forage fiss behind the solution hydrotropy, it is 4.5%～10.0% that described scutellaria composite immune reinforcing agent accounts for total weight percent; Perhaps, make pellet by the consumption of feedstuff adding 0.2kg～1.0kg claim 1 per ton or 2 or 3 described scutellaria composite immune reinforcing agents.
7. scutellaria composite immune reinforcing agent according to claim 1 and 2 is used for preparing prevention or the medicine of shell-fish infectious disease or the application of feedstuff are cultured in treatment.
8. scutellaria composite immune reinforcing agent according to claim 7 is used for preparing prevention or the medicine of shell-fish pathogenicity disease or the application of feedstuff are cultured in treatment, and it is characterized in that: described infectious disease is the pathogenicity infectious disease.
9. scutellaria composite immune reinforcing agent according to claim 7 is used for preparing prevention or the medicine of shell-fish pathogenicity disease or the application of feedstuff are cultured in treatment, it is characterized in that: described infectious disease is the pathogenicity disease that the infection of white spot syndrome virus infection, the whirlpool whipworm infection of blood ovum and other ciliate, vibrio causes.
10. scutellaria composite immune reinforcing agent according to claim 7 is used for preparing prevention or the medicine of shell-fish infectious disease or the application of feedstuff are cultured in treatment, and it is characterized in that: described breed shell-fish is mud crab, swimming crab, Penaeus vannamei or dusky white prawn.
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| CN104273345A (en) * | 2014-08-14 | 2015-01-14 | 浙江省淡水水产研究所 | Complex immunity enhancer for Macrobra'chiumrosenbergiideman and application of complex immunity enhancer |
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| CN112617056A (en) * | 2020-12-18 | 2021-04-09 | 马鞍山市徽农农业科技发展有限公司 | Crab feed for molting stage of crabs and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102687813A (en) * | 2012-04-23 | 2012-09-26 | 浙江省海洋开发研究院 | Nutrient and green feed capable of enhancing immune function of mud crab and preparing method thereof |
| CN104273345A (en) * | 2014-08-14 | 2015-01-14 | 浙江省淡水水产研究所 | Complex immunity enhancer for Macrobra'chiumrosenbergiideman and application of complex immunity enhancer |
| CN104273345B (en) * | 2014-08-14 | 2019-03-12 | 浙江省淡水水产研究所 | A kind of Macrobrachium rosenbergii composite immune reinforcing agent and its application |
| CN108478587A (en) * | 2018-04-08 | 2018-09-04 | 广州市汇鑫动物药业有限公司 | Application of the scutelloside in preparing anti-Cryptosporidium baileyi drug |
| CN108850704A (en) * | 2018-06-26 | 2018-11-23 | 广东海因特生物技术集团有限公司 | Chinese herbal feed additive and its preparation method and application for Penaeus Vannmei |
| CN112617056A (en) * | 2020-12-18 | 2021-04-09 | 马鞍山市徽农农业科技发展有限公司 | Crab feed for molting stage of crabs and preparation method thereof |
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